Publications by authors named "Lei Sun"

1,666 Publications

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Rapidly detecting fennel origin of the near-infrared spectroscopy based on extreme learning machine.

Sci Rep 2022 Aug 10;12(1):13593. Epub 2022 Aug 10.

College of Information Science and Engineering, Xinjiang University, Urumqi, 830046, China.

Fennel contains many antioxidant and antibacterial substances, and it has very important applications in food flavoring and other fields. The kinds and contents of chemical substances in fennel vary from region to region, which can affect the taste and efficacy of the fennel and its derivatives. Therefore, it is of great significance to accurately classify the origin of the fennel. Recently, origin detection methods based on deep networks have shown promising results. However, the existing methods spend a relatively large time cost, a drawback that is fatal for large amounts of data in practical application scenarios. To overcome this limitation, we explore an origin detection method that guarantees faster detection with classification accuracy. This research is the first to use the machine learning algorithm combined with the Fourier transform-near infrared (FT-NIR) spectroscopy to realize the classification and identification of the origin of the fennel. In this experiment, we used Rubberband baseline correction on the FT-NIR spectral data of fennel (Yumen, Gansu and Turpan, Xinjiang), using principal component analysis (PCA) for data dimensionality reduction, and selecting extreme learning machine (ELM), Convolutional Neural Network (CNN), recurrent neural network (RNN), Transformer, generative adversarial networks (GAN) and back propagation neural network (BPNN) classification model of the company realizes the classification of the sample origin. The experimental results show that the classification accuracy of ELM, RNN, Transformer, GAN and BPNN models are above 96%, and the ELM model using the hardlim as the activation function has the best classification effect, with an average accuracy of 100% and a fast classification speed. The average time of 30 experiments is 0.05 s. This research shows the potential of the machine learning algorithm combined with the FT-NIR spectra in the field of food production area classification, and provides an effective means for realizing rapid detection of the food production area, so as to merchants from selling shoddy products as good ones and seeking illegal profits.
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http://dx.doi.org/10.1038/s41598-022-17810-yDOI Listing
August 2022

Associations between isoflavone exposure and reproductive damage in adult males: evidence from human and model system studies.

Biol Reprod 2022 Aug 10. Epub 2022 Aug 10.

Key Lab of Medical Protection for Electromagnetic Radiation, Ministry of Education of China, Institute of Toxicology, College of Preventive Medicine, Third Military Medical University, Gaotanyan St 30, Chongqing 400038, China.

It's controversial whether exposure to isoflavones, constituents of certain plants such as soy bean, exerts male reproductive toxicity. This study was designed to investigate whether isoflavone exposure during adulthood could have deleterious impacts on male reproductive health by the cross-sectional study, animal experiments, and in vitro tests. In the cross-sectional study, we observed that urinary isoflavones were not significantly associated with semen quality including sperm concentrations, sperm count, progressive motility, and total motility, respectively (All P-value for trend>0.05). However, negative associations were found between plasma testosterone and urinary Σisoflavones, genistein, glycitein, and dihydrodaidzein (all P-value for trend <0.05). In the animal experiments, serum and intratesticular testosterone levels were decreased in mice exposed to several dosages of genistein. Genistein administration caused up-regulation of estrogen receptor alpha (ERα) and down-regulation of cytochrome P45017A1 (CYP17A1) protein levels in testes of mice. However, genistein treatment during adulthood did not induce appreciable structural damages of reproductive system in mice. In vitro tests, we observed that genistein of different dosages (0.01, 2.5, 10 μM) caused a concentration dependent inhibition of testosterone production by TM3 Leydig cells (half-maximal inhibitory concentration = 3.796 nM, P < 0.05). Elevated protein expression of ERα and decreased mRNA/protein level of CYP17A1 were also observed in genistein-treated cells. Protein level of CYP17A1 and testosterone concentration were significantly restored in the ERα siRNA-transfected cells, compared to cells that treated with genistein alone (P < 0.05). The results demonstrate that exposure to isoflavones during adulthood may be associated with alterations of reproductive hormones. Particularly for genistein, which inhibits testosterone biosynthesis through up-regulation of ERα in Leydig cells of mice, might induce the disruption of testosterone production in human. The present study provides novel perspective into potential targets for male reproductive compromise induced by isoflavone exposure.
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http://dx.doi.org/10.1093/biolre/ioac157DOI Listing
August 2022

Phytochemical and pharmacological review of diterpenoids from the genus Euphorbia Linn (2012-2021).

J Ethnopharmacol 2022 Aug 6;298:115574. Epub 2022 Aug 6.

Key Laboratory of Research and Development of Natural Product from Li Folk Medicine of Hainan Province, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China; Hainan Institute for Tropical Agricultural Resources, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, PR China. Electronic address:

Ethnopharmacological Relevance: Euphorbia is one of the major genera in angiosperms, which is widely distributed all over the world, including Asia, Africa and Central and South America. The roots or tubers of Euphorbia are famous for medicinal purposes, especially in China. Many of them, such as Euphorbia pekinensis Rupr, Euphorbia fischeriana Steud and Euphorbia Kansui S.L.Liou ex S.B.Ho. . are used as Chinese herbal medicines.

Aim Of The Study: This paper reviews the diterpenoids isolated from the genus Euphorbia species and the pharmacological activities of these compounds to evaluate its traditional use and potential future development.

Materials And Methods: Information on the studies of the genus Euphorbia Linn was collected from scientific journals, books and reports via library and electronic data search (Scifinder, Web of Science, PubMed, Elsevier, Scopus, Google Scholar, Springer, Science Direct, Wiley, ACS, CNKI and Kew Plants of the Word Online). Meanwhile, it was also obtained from published works of material medica, folk records, ethnophmacological literatures, Ph.D. and Masters dissertations.

Results: Known as the main constituents of the genus Euphorbia Linn, Diterpenoids possess many pharmacological properties such as anti-inflammation, antiviral activities and cytotoxicity. To date, various types of diterpenoids were identified from this genus, including isopimarane, rosane, abietane, ent-kaurane, ent-atisane. cembrane, casbane, lathyrane, myrsinane, jatropholane, tigliane, ingenane, jatrophane, paraliane, pepluane, and euphoractin.

Conclusions: This review describes 14 types of diterpenoid isolated from 45 Euphorbia species from 2012 to 2021, a total of 615 compounds. Among them, mainly include jatrophane (171), lathyrane (92), myrsinane (62), abietane (70), ent-atisane (36), ent-kaurane (7), tigliane (26) and ingenane (19). The possible biological pathways of these compounds were presumed. At the same time, more than 10 biological activities of these compounds were summarized, such as anti-inflammation, antiviral activities and cytotoxicity.
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http://dx.doi.org/10.1016/j.jep.2022.115574DOI Listing
August 2022

A 33-residue peptide tag increases solubility and stability of Escherichia coli produced single-chain antibody fragments.

Nat Commun 2022 Aug 8;13(1):4614. Epub 2022 Aug 8.

Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai, China.

Single-chain variable fragments (scFvs), composed of variable domains of heavy and light chains of an antibody joined by a linker, share antigen binding capacity with their parental antibody. Due to intrinsically low solubility and stability, only two Escherichia coli-produced scFvs have been approved for therapy. Here we report that a 33-residue peptide, termed P17 tag, increases the solubility of multiple scFvs produced in Escherichia coli SHuffle strain by up to 11.6 fold. Hydrophilic sequence, especially charged residues, but not the predicted α-helical secondary structure of P17 tag, contribute to the solubility enhancement. Notably, the P17 tag elevates the thermostability of scFv as efficiently as intra-domain disulfide bonds. Moreover, a P17-tagged scFv targeting hepatitis B virus surface proteins shows over two-fold higher antigen-binding affinity and virus-neutralizing activity than the untagged version. These data strongly suggest a type I intramolecular chaperone-like activity of the P17 tag. Hence, the P17 tag could benefit the research, production, and application of scFv.
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http://dx.doi.org/10.1038/s41467-022-32423-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9359998PMC
August 2022

Robust secret image sharing scheme resistance to maliciously tampered shadows by AMBTC and quantization.

Gene Expr Patterns 2022 Aug 5:119267. Epub 2022 Aug 5.

National University of Defense Technology, Hefei, 230037, China; Anhui Key Laboratory of Cyberspace Security Situation Awareness and Evaluation, Hefei, 230037, China.

For (k, n)-threshold secret image sharing (SIS) scheme, only k or more than k complete parts can recover the secret information, and the correct image cannot be obtained if the count of shadow images is not enough or the shadow images are damaged. The existing schemes are weak in resisting large-area shadow image tampering. In this paper, we propose a robust secret image sharing scheme resisting to maliciously tampered shadow images by Absolute Moment Block Truncation Coding (AMBTC) and quantization (RSIS-AQ). The secret image is successively compressed in two ways: AMBTC and quantization. The sharing shadow images contain the sharing results of both compressed image from different parts, so that even the shadow images are faced with large-scale area of malicious tampering, the secret image can be recovered with acceptable visual quality. Compared with related works, our scheme can resist larger area of tampering and yield better recovered image visual quality. The experimental results prove the effectiveness of our scheme.
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http://dx.doi.org/10.1016/j.gep.2022.119267DOI Listing
August 2022

Association of Metabolic Dysfunction-Associated Fatty Liver Disease With Left Ventricular Diastolic Function and Cardiac Morphology.

Front Endocrinol (Lausanne) 2022 19;13:935390. Epub 2022 Jul 19.

Hangzhou Institute of Cardiovascular Diseases, Affiliated Hospital of Hangzhou Normal University, Hangzhou Normal University, Hangzhou, China.

Background And Aim: Non-alcoholic fatty liver disease (NAFLD) is closely related to cardiovascular diseases (CVD). A newly proposed definition is metabolic dysfunction-associated fatty liver disease (MAFLD), which was changed from NAFLD. The clinical effect of this change on abnormalities of cardiac structure and function is yet unknown. We aimed to examine whether MAFLD is associated with left ventricular (LV) diastolic dysfunction (LVDD) and cardiac remolding and further identify the impact of different subgroups and severity of MAFLD.

Method: We evaluated 228 participants without known CVDs. Participants were categorized by the presence of MAFLD and the normal group. Then, patients with MAFLD were subclassified into three subgroups: MAFLD patients with diabetes (diabetes subgroup), overweight/obesity patients (overweight/obesity subgroup), and lean/normal-weight patients who had two metabolic risk abnormalities (lean metabolic dysfunction subgroup). Furthermore, the severity of hepatic steatosis was assessed by transient elastography (FibroScan) with a controlled attenuation parameter (CAP), and patients with MAFLD were divided into normal, mild, moderate, and severe hepatic steatosis groups based on CAP value. Cardiac structure and function were examined by echocardiography.

Results: LVDD was significantly more prevalent in the MAFLD group (24.6% vs. 60.8%,  < 0.001) compared to the normal group. The overweight subgroup and diabetes subgroup were significantly associated with signs of cardiac remolding, including interventricular septum thickness, LV posterior wall thickness, left atrial diameter (all  < 0.05), relative wall thickness, and LV mass index (all < 0.05). Additionally, moderate-to-to severe steatosis patients had higher risks for LVDD and cardiac remolding (all -values < 0.05).

Conclusion: MAFLD was associated with LVDD and cardiac remolding, especially in patients with diabetes, overweight patients, and moderate-to-to severe steatosis patients. This study provides theoretical support for the precise prevention of cardiovascular dysfunction in patients with MAFLD.
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http://dx.doi.org/10.3389/fendo.2022.935390DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9345495PMC
August 2022

Rational Design of Synergistic Structure Between Single-Atoms and Nanoparticles for CO Hydrogenation to Formate Under Ambient Conditions.

Front Chem 2022 19;10:957412. Epub 2022 Jul 19.

Institute of Molecular Sciences and Engineering, Institute of Frontier and Interdisciplinary Science, Shandong University, Qingdao, China.

Single-atom catalysts (SACs) as the new frontier in heterogeneous catalysis have attracted increasing attention. However, the rational design of SACs with high catalytic activities for specified reactions still remains challenging. Herein, we report the rational design of a Pd-Pd synergistic structure on -pyridinedicarbonitrile-derived covalent triazine framework () as an efficient active site for CO hydrogenation to formate under ambient conditions. Compared with the catalysts mainly comprising Pd and Pd, this hybrid catalyst presented significantly improved catalytic activity. By regulating the ratio of Pd to Pd, we obtained the optimal catalytic activity with a formate formation rate of 3.66 mol·mol ·h under ambient conditions (30°C, 0.1 MPa). Moreover, as a heterogeneous catalyst, this hybrid catalyst is easily recovered and exhibits about a 20% decrease in the catalytic activity after five cycles. These findings are significant in elucidating new rational design principles for CO hydrogenation catalysts with superior activity and may open up the possibilities of converting CO under ambient conditions.
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http://dx.doi.org/10.3389/fchem.2022.957412DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9343707PMC
July 2022

Structural Study of SARS-CoV-2 Antibodies Identifies a Broad-Spectrum Antibody That Neutralizes the Omicron Variant by Disassembling the Spike Trimer.

J Virol 2022 Aug 4:e0048022. Epub 2022 Aug 4.

Shanghai Institute of Infectious Disease and Biosecurity, Shanghai Key Laboratory of Medical Epigenetics and Institutes of Biomedical Sciences, Fudan University, Shanghai, China.

The continuous emergence of novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants poses new challenges in the fight against the coronavirus disease 2019 (COVID-19) pandemic. The newly emerging Omicron strain caused serious immune escape and raised unprecedented concern all over the world. The development of an antibody targeting a conserved and universal epitope is urgently needed. A subset of neutralizing antibodies (NAbs) against COVID-19 from convalescent patients were isolated in our previous study. In this study, we investigated the accommodation of these NAbs to SARS-CoV-2 variants of concern (VOCs), revealing that IgG 553-49 neutralizes pseudovirus of the SARS-CoV-2 Omicron variant. In addition, we determined the cryo-electron microscopy (cryo-EM) structure of the SARS-CoV-2 spike (S) protein complexed with three monoclonal antibodies targeting different epitopes, including 553-49, 553-15, and 553-60. Notably, 553-49 targets a novel conserved epitope and neutralizes the virus by disassembling S trimers. IgG 553-15, an antibody that neutralizes all of the VOCs except Omicron, cross-links two S trimers to form a trimer dimer, demonstrating that 553-15 neutralizes the virus by steric hindrance and virion aggregation. These findings suggest the potential to develop 553-49 and other antibodies targeting this highly conserved epitope as promising therapeutic reagents for COVID-19. The emergence of the Omicron strain of SARS-CoV-2 caused higher immune escape, raising unprecedented concerns about the effectiveness of antibody therapies and vaccines. In this study, we identified a SARS-CoV-2 neutralizing antibody, 553-49, which neutralizes all variants by targeting a completely conserved novel epitope. In addition, we revealed that IgG 553-15 neutralizes SARS-CoV-2 by cross-linking virions and that 553-60 functions by blocking receptor binding. Comparison of different receptor binding domain (RBD) epitopes revealed that the 553-49 epitope is hidden in the S trimer and keeps a high degree of conservation during SARS-CoV-2 evolution, making 553-49 a promising therapeutic reagent against the emerging Omicron and future variants of SARS-CoV-2.
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http://dx.doi.org/10.1128/jvi.00480-22DOI Listing
August 2022

The Predictive Value of Pretreatment Lactate Dehydrogenase and Derived Neutrophil-to-Lymphocyte Ratio in Advanced Non-Small Cell Lung Cancer Patients Treated With PD-1/PD-L1 Inhibitors: A Meta-Analysis.

Front Oncol 2022 18;12:791496. Epub 2022 Jul 18.

Department of Respiratory and Critical Care Medicine, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, China.

Background: The Lung Immune Prognostic Index (LIPI) combines the lactate dehydrogenase (LDH) level and the derived neutrophil-to-lymphocyte ratio (dNLR). A lot of studies have shown that LDH and dNLR are associated with the prognosis of advanced non-small cell lung cancer (NSCLC) in patients treated with programmed cell death protein 1 (PD-1) or programmed death-ligand 1 (PD-L1) inhibitors. However, previous results were inconsistent, and the conclusions remain unclear. This meta-analysis aimed to investigate the predictive value of pretreatment LDH and dNLR for NSCLC progression in patients treated with PD-1/PD-L1 inhibitors.

Methods: PubMed, Embase, and the Cochrane Library were searched by two researchers independently for related literature before March 2020. Hazard ratios (HRs) with 95% confidence intervals (CIs) for progression-free survival (PFS) and overall survival (OS) were extracted to assess the predictive value of LDH and dNLR. STATA 15. 0 was used to perform the meta-analysis.

Results: A total of 3,429 patients from 26 studies were included in this meta-analysis. The results revealed that high pretreatment LDH was related to poor OS (HR = 1.19, 95%CI = 1.11-1.24,  < 0.001), but not closely related to poor PFS (HR = 1.02, 95%CI = 1.00-1.04,  = 0.023 < 0.05). The pooled results for dNLR suggested that high pretreatment dNLR was related to poor OS (HR = 1.55, 95%CI = 1.33-1.80,  < 0.001) and PFS (HR = 1.33, 95%CI = 1.16-1.54,  < 0.001).

Conclusion: Both pretreatment LDH and dNLR have the potential to serve as peripheral blood biomarkers for patients with advanced NSCLC treated with PD-1/PD-L1 inhibitors. However, more studies on LDH are needed to evaluate its predictive value for PFS in patients with NSCLC.
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http://dx.doi.org/10.3389/fonc.2022.791496DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9340347PMC
July 2022

Krill Oil Turns Off TGF-β1 Profibrotic Signaling in the Prevention of Diabetic Nephropathy.

J Agric Food Chem 2022 Aug 2. Epub 2022 Aug 2.

Research Center of Translational Medicine, Jinan Central Hospital, Shandong University, 105 Jiefang Rd., Jinan, Shandong 250013, China.

Diabetic nephropathy (DN), a severe microvascular complication of diabetes mellitus (DM), results in high mortality due to the lack of effective interventions. The current study investigated the preventive effect of krill oil (KO) on DN using a type 2 DM mouse model induced by streptozotocin and high-fat diet for 24 weeks. The diabetic mice developed albuminuria, mesangial matrix accumulation, glomerular hypertrophy, and fibrosis formation, with an increase in renal proinflammatory, oxidative and profibrotic gene expression. KO significantly prevented these effects but did not improve hyperglycemia and glucose intolerance. In high-glucose-treated mesangial cells (MCs), KO preferably modulated TGF-β1 signaling as revealed by RNA-sequencing. In TGF-β1-treated MCs, KO abolished SMAD2/3 phosphorylation and nuclear translocation and activated 7 gene expression. The action of KO on the SMADs was confirmed in the diabetic kidneys. Therefore, KO may prevent DN predominantly by suppressing the TGF-β1 signaling pathway.
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http://dx.doi.org/10.1021/acs.jafc.2c02850DOI Listing
August 2022

Identification and Quantification of Chlorogenic Acids from the Root Bark of by UHPLC-Q-Exactive Orbitrap Mass Spectrometry.

ACS Omega 2022 Jul 11;7(29):25675-25685. Epub 2022 Jul 11.

School of Pharmaceutical Sciences & Hunan University of Medicine, Huaihua 418000, P.R. China.

The purpose of this study is to identify and quantify the chlorogenic acids (CGAs) from the root bark of , which is conventionally regarded as a tonic in folk Chinese Traditional medicine. The effective methods for identification and quantification analysis of CGAs were developed based on ultra high performance liquid chromatography-Q-exactive orbitrap mass spectrometry (UHPLC-Q-Orbitrap MS) in parallel reaction monitoring (PRM) and selected reaction monitoring (SIM), which showed high sensitivity and resolution for screening and quantifying compounds. The root bark of was extracted under ultrasonication with 70% methanol. Ultimately, a for total of 70 CGAs, 64 of these were tentatively identified for the first time. Moreover, a methodological study of seven kinds of CGAs was carried out. The proposed procedure was optimized and validated in terms of selectivity, linearity of analytical curves ( > 0.990), accuracy (recovery range from 96.7 to 105%), and repeatability (relative standard deviation <5%). Then it was applied to determine the content of the CGAs in roots from 66 of different batches. The total CGAs was quantified in a range between 2.150 and 33.51 mg/g, which could be considered as excellent source of natural bioactive compound. The result was extremely useful for understanding the bioactive substance and quality control of in depth.
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http://dx.doi.org/10.1021/acsomega.2c02899DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9330223PMC
July 2022

Transgressive Potential Prediction and Optimal Cross Design of Seed Protein Content in the Northeast China Soybean Population Based on Full Exploration of the QTL-Allele System.

Front Plant Sci 2022 12;13:896549. Epub 2022 Jul 12.

Soybean Research Institute/MARA National Center for Soybean Improvement/MARA Key Laboratory of Biology and Genetic Improvement of Soybean (General), Nanjing Agricultural University, Nanjing, China.

Northeast China is a major soybean production region in China. A representative sample of the Northeast China soybean germplasm population (NECSGP) composed of 361 accessions was evaluated for their seed protein content (SPC) in Tieling, Northeast China. This SPC varied greatly, with a mean SPC of 40.77%, ranging from 36.60 to 46.07%, but it was lower than that of the Chinese soybean landrace population (43.10%, ranging from 37.51 to 50.46%). The SPC increased slightly from 40.32-40.97% in the old maturity groups (MG, MGIII + II + I) to 40.93-41.58% in the new MGs (MG0 + 00 + 000). The restricted two-stage multi-locus genome-wide association study (RTM-GWAS) with 15,501 SNP linkage-disequilibrium block (SNPLDB) markers identified 73 SPC quantitative trait loci (QTLs) with 273 alleles, explaining 71.70% of the phenotypic variation, wherein 28 QTLs were new ones. The evolutionary changes of QTL-allele structures from old MGs to new MGs were analyzed, and 97.79% of the alleles in new MGs were inherited from the old MGs and 2.21% were new. The small amount of new positive allele emergence and possible recombination between alleles might explain the slight SPC increase in the new MGs. The prediction of recombination potentials in the SPC of all the possible crosses indicated that the mean of SPC overall crosses was 43.29% (+2.52%) and the maximum was 50.00% (+9.23%) in the SPC, and the maximum transgressive potential was 3.93%, suggesting that SPC breeding potentials do exist in the NECSGP. A total of 120 candidate genes were annotated and functionally classified into 13 categories, indicating that SPC is a complex trait conferred by a gene network.
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http://dx.doi.org/10.3389/fpls.2022.896549DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9317943PMC
July 2022

Biomechanical evaluation of the reconstruction of the calcar femorale in femoral neck fractures: a comparative finite element analysis.

J Int Med Res 2022 Jul;50(7):3000605221112043

Tianjin Hospital, Tianjin University, No. 406 Jiefang South Road, Tianjin, People's Republic of China.

Objective: Femoral neck fractures are common. We evaluated the biomechanical performance of an internal fixation method based on traditional three cannulated screws (3CS) inserted from below the fracture in the direction of the calcar femorale in the treatment of Pauwels III femoral neck fracture.

Methods: We constructed and evaluated a three-dimensional model of a Pauwels III femoral neck fracture with four models of internal fixation (3CS, and 150°, 155°, and 160° nailing angles) for reconstruction of the calcar femorale, by finite element analysis (FEA).

Results: The peak stress values at the fracture ends in the 3CS, 150°, 155°, and 160° nailing angle models were 30.052 MPa, 33.382 MPa, 34.012 MPa, and 29.858 MPa; peak stress values for internal fixed stress were 315.121 MPa, 228.819 MPa, 198.173 MPa, and 208.798 MPa; and the maximum displacement of the femoral head was 13.190 mm, 13.183 mm, 12.443 mm, and 12.896 mm, respectively.

Conclusion: FEA showed that the new nailing methods and the 160° nailing angle for reconstruction of the calcar femorale showed better performance in resisting shearing force for Pauwels III femoral neck fracture, with better mechanical properties, than those with the other three models. These findings can provide a clinical reference.
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http://dx.doi.org/10.1177/03000605221112043DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9340950PMC
July 2022

Role of Glycolysis/Gluconeogenesis and HIF-1 Signaling Pathways in Rats with Dental Fluorosis Integrated Proteomics and Metabolomics Analysis.

Int J Mol Sci 2022 Jul 27;23(15). Epub 2022 Jul 27.

Department of Environmental Health, School of Public Health, Zhengzhou University, 100 Kexue Avenue, Zhengzhou 450001, China.

Fluoride is widely distributed, and excessive intake will lead to dental fluorosis. In this study, six offspring rats administrated 100 mg/L sodium fluoride were defined as the dental fluorosis group, and eight offspring rats who received pure water were defined as the control group. Differentially expressed proteins and metabolites extracted from peripheral blood were identified using the liquid chromatography tandem mass spectrometry and gas chromatography mass spectrometry, with the judgment criteria of fold change >1.2 or <0.83 and < 0.05. A coexpression enrichment analysis using OmicsBean was conducted on the identified proteins and metabolites, and a false discovery rate (FDR) < 0.05 was considered significant. Human Protein Atlas was used to determine the subcellular distribution of hub proteins. The Gene Cards was used to verify results. A total of 123 up-regulated and 46 down-regulated proteins, and 12 up-regulated and 2 down-regulated metabolites were identified. The significant coexpression pathways were the HIF-1 (FDR = 1.86 × 10) and glycolysis/gluconeogenesis (FDR = 1.14 × 10). The results of validation analysis showed the proteins related to fluorine were mainly enriched in the cytoplasm and extrinsic component of the cytoplasmic side of the plasma membrane. The HIF-1 pathway (FDR = 1.01 × 10) was also identified. Therefore, the HIF-1 and glycolysis/gluconeogenesis pathways were significantly correlated with dental fluorosis.
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http://dx.doi.org/10.3390/ijms23158266DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9332816PMC
July 2022

Brown adipose tissue influences adiponectin and thyroid hormone changes during Graves' disease therapy.

Adipocyte 2022 Dec;11(1):389-400

Lee Kong Chian School of Medicine, Nanyang Technological University (NTU), Singapore, Singapore.

Thyroid hormones (TH), adiponectin and brown adipose tissue (BAT) are regulators of energy homoeostasis. Influence of BAT activity on the relationship between TH and adiponectin remains unexplored. The aim of the study was to identify the relationship between TH and adiponectin and to clarify the impact of active BAT on the metabolic effects of adiponectin before and after the correction of thyrotoxicosis. Twenty-one patients with newly diagnosed hyperthyroidism from Graves' disease were recruited. A titration dosing regimen of thionamide anti-thyroid drug (ATD) was used to establish euthyroidism over 12-24 weeks. Anthropometric, biochemical and adipocytokine parameters were measured before and after control of hyperthyroidism. BAT activity was quantified by fusion 18 F-fluorodeoxyglucose (18 F-FDG) PET/MR imaging, and patients were grouped based on BAT status. Plasma adiponectin level was significantly increased following correction of hyperthyroidism in the overall sample. Free thyroxine (FT4) was also identified as a predictor of adiponectin level in thyroid dysfunction. However, significant changes in adiponectin level and correlations involving adiponectin were absent in BAT-positive patients but maintained in BAT-negative patients. BAT activity diminishes the correlative relationship with body composition and abolishes TH and adiponectin relationships when transitioning from a hyperthyroid to euthyroid state.
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http://dx.doi.org/10.1080/21623945.2022.2104509DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9336474PMC
December 2022

Effects of Fermented Green Tea Waste Extract Gels on Oxidative Damage in Short-Term Passive Smoking Mice.

Gels 2022 Jul 22;8(8). Epub 2022 Jul 22.

School of Food and Bioengineering, Hefei University of Technology, Hefei 230009, China.

Passive smoking is extensively studied because of its harmfulness to human health. In this study, the effects of fermented green tea waste extract gels (GTEG) on oxidative damage in mice exposed to short-term cigarette smoke (CS) were investigated. The GTEG is prepared from green tea waste extract and microbial transglutaminase (MTGase). The lung injury model of mice was established through passive smoking for 5 days. The experimental results revealed the following findings. (1) The GTEG induced by MTGase has obvious gel properties; (2) GTEG has strong biological activity and antioxidant properties in vitro; (3) The passive smoking model was established successfully; specifically, the lung tissue of the model mice exhibited inflammatory symptoms, oxidative stress response appeared in their bodies, and their inflammatory indicators increased; (4) Compared with the passive smoking model group, the mice, which were exposed to CS and received GTEG treatment, exhibited increased food intake and body weight; increased total superoxide dismutase and glutathione peroxidase activity in serum; significant decreases ( < 0.05) in the content levels of the inflammatory factors malondialdehyde, interleukin (IL)-6, and tumor necrosis factor α (TNF-α); and inhibited expression of IL-6, IL-33, TNF-α, and IL-1β inflammatory genes. The results indicated that taking GTEG can relieve the oxidative stress injury of mice caused by short-term CS and has antioxidant properties.
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http://dx.doi.org/10.3390/gels8080461DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9332167PMC
July 2022

The Application of Hollow Fiber Cartridge in Biomedicine.

Pharmaceutics 2022 Jul 18;14(7). Epub 2022 Jul 18.

National Reference Laboratory of Veterinary Drug Residues, Huazhong Agricultural University, Wuhan 430070, China.

The hollow fiber cartridge has the advantages of good semi-permeability, high surface area to volume ratio, convenient operation, and so on. Its application in chemical analysis, drug in vitro experiment, hemodialysis, and other fields has been deeply studied. This paper introduces the basic structure of hollow fiber cartridge, compares the advantages and disadvantages of a hollow fiber infection model constructed by a hollow fiber cartridge with traditional static model and animal infection model and introduces its application in drug effects, mechanism of drug resistance, and evaluation of combined drug regimen. The principle and application of hollow fiber bioreactors for cell culture and hollow fiber dialyzer for dialysis and filtration were discussed. The hollow fiber cartridge, whether used in drug experiments, artificial liver, artificial kidney, etc., has achieved controllable experimental operation and efficient and accurate experimental results, and will provide more convenience and support for drug development and clinical research in the future.
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http://dx.doi.org/10.3390/pharmaceutics14071485DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9316653PMC
July 2022

Understanding Chinese international students' perception of flu vaccination on U.S. college campuses.

J Am Coll Health 2022 Jul 26:1-9. Epub 2022 Jul 26.

Department of Educational and Psychological Studies, University of Miami, Coral Gables, Florida, USA.

: This study examines Chinese international students' underlying beliefs and overall perception of the flu vaccination to inform effective health promotion efforts on U.S. college campuses. : Data were collected in March 2020. Participants ( = 189) were recruited via email at a southeastern university in the U.S. : Incorporating theory recommendations and recent vaccine-related research findings, this study designs a Four-Factor Measurement Model through confirmatory factor analysis (CFA) to examine Chinese international students' perception of flu vaccination. : Chinese international students' health beliefs, particularly their perceived susceptibility of the influenza virus, was the most important factor to explain their perception of flu vaccination. While normative beliefs were the least influential factor to account for Chinese international students' perception of flu vaccination, these students valued healthcare providers' opinions immensely. : This study suggests the Four-Factor Measurement Model will help measure Chinese international students' perception of flu vaccination and can be applied to future vaccine-related research.
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http://dx.doi.org/10.1080/07448481.2022.2103381DOI Listing
July 2022

Accuracy benchmark of the GeneMind GenoLab M sequencing platform for WGS and WES analysis.

BMC Genomics 2022 Jul 22;23(1):533. Epub 2022 Jul 22.

GeneMind Biosciences Company Limited, Shenzhen, China.

Background: GenoLab M is a recently developed next-generation sequencing (NGS) platform from GeneMind Biosciences. To establish the performance of GenoLab M, we present the first report to benchmark and compare the WGS and WES sequencing data of the GenoLab M sequencer to NovaSeq 6000 and NextSeq 550 platform in various types of analysis. For WGS, thirty-fold sequencing from Illumina NovaSeq platform and processed by GATK pipeline is currently considered as the golden standard. Thus this dataset is generated as a benchmark reference in this study.

Results: GenoLab M showed an average of 94.62% of Q20 percentage for base quality, while the NovaSeq was slightly higher at 96.97%. However, GenoLab M outperformed NovaSeq or NextSeq at a duplication rate, suggesting more usable data after deduplication. For WGS short variant calling, GenoLab M showed significant accuracy improvement over the same depth dataset from NovaSeq, and reached similar accuracy to NovaSeq 33X dataset with 22x depth. For 100X WES, the F-score and Precision in GenoLab M were higher than NovaSeq or NextSeq, especially for InDel calling.

Conclusions: GenoLab M is a promising NGS platform for high-performance WGS and WES applications. For WGS, 22X depth in the GenoLab M sequencing platform offers a cost-effective alternative to the current mainstream 33X depth on Illumina.
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http://dx.doi.org/10.1186/s12864-022-08775-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9308344PMC
July 2022

Progress and prospect of technical and regulatory challenges on tissue-engineered cartilage as therapeutic combination product.

Bioact Mater 2023 Feb 27;20:501-518. Epub 2022 Jun 27.

State Key Laboratory of Tribology, Tsinghua University, Beijing, PR China.

Hyaline cartilage plays a critical role in maintaining joint function and pain. However, the lack of blood supply, nerves, and lymphatic vessels greatly limited the self-repair and regeneration of damaged cartilage, giving rise to various tricky issues in medicine. In the past 30 years, numerous treatment techniques and commercial products have been developed and practiced in the clinic for promoting defected cartilage repair and regeneration. Here, the current therapies and their relevant advantages and disadvantages will be summarized, particularly the tissue engineering strategies. Furthermore, the fabrication of tissue-engineered cartilage under research or in the clinic was discussed based on the traid of tissue engineering, that is the materials, seed cells, and bioactive factors. Finally, the commercialized cartilage repair products were listed and the regulatory issues and challenges of tissue-engineered cartilage repair products and clinical application would be reviewed.
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http://dx.doi.org/10.1016/j.bioactmat.2022.06.015DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9253051PMC
February 2023

Human umbilical cord blood-derived MSCs trans-differentiate into endometrial cells and regulate Th17/Treg balance through NF-κB signaling in rabbit intrauterine adhesions endometrium.

Stem Cell Res Ther 2022 07 15;13(1):301. Epub 2022 Jul 15.

Department of Obstetrics and Gynecology, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou, Henan, People's Republic of China.

Purpose: The fundamental cause of intrauterine adhesions (IUAs) is the destruction and reduction in stem cells in endometrial basal layer, resulting in endometrial reconstruction very difficult. The purpose of this study was to investigate the effects and underlying mechanism of human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) on the endometrial reconstruction after transplantation.

Methods: hUCB-MSCs were isolated and identified by flow cytometry, osteogenic, adipogenic and chondrogenic differentiation assays. The rabbit IUA models were established and set five groups (control, 14/28th day after surgery, estrogen and hUCB-MSCs treatment). The number of endometrial glands and the fibrosis rate were evaluated using HE and Masson staining, respectively. Endometrial proliferation, angiogenesis and inflammation were evaluated by immunohistochemical staining of ER, Ki-67and TGF-β1, respectively. Single-cell RNA sequencing (scRNA-seq) was applied to explore the cell differentiation trajectory after hUCB-MSCs transplanted into IUA endometrium. Finally, molecular mechanism of hUCB-MSCs repairing damaged endometrium was investigated by RNA sequencing, qRT-PCR and Western blot assays.

Results: After transplantation of the hUCB-MSCs, the increase in endometrial gland number, estrogen receptor (ER) and Ki-67 expression, and the decrease in fibrosis rate and TGF-β expression (P < 0.05), suggested the endometrial repair, angiogenesis and inflammatory suppression. The therapeutic effect of hUCB-MSCs was significantly improved compared with 28th day after surgery and estrogen group. ScRNA-seq demonstrated that the transplanted hUCB-MSCs can trans-differentiate into endometrial cells: epithelial, fibroblast and macrophage. RNA sequencing of six IUA samples combined with qRT-PCR and Western blot assays further revealed that hUCB-MSCs may regulate Th17/Treg balance through NF-κB signaling, thus inhibiting the immune response of damaged endometrium.

Conclusions: Our study demonstrated that hUCB-MSCs can repair damaged endometrium through trans-differentiation, immunomodulatory capacities and NF-κB signaling, suggesting the treatment value of hUCB-MSCs in IUA.
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http://dx.doi.org/10.1186/s13287-022-02990-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9284747PMC
July 2022

Risk factors for Drug-resistant Epilepsy (DRE) and a nomogram model to predict DRE development in post-traumatic epilepsy patients.

CNS Neurosci Ther 2022 Jul 12. Epub 2022 Jul 12.

Department of Neurology, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.

Objectives: To identify factors affecting the development of drug-resistant epilepsy (DRE), and establish a reliable nomogram to predict DRE development in post-traumatic epilepsy (PTE) patients.

Methods: This study conducted a retrospective clinical analysis in patients with PTE who visited the Epilepsy Center, Beijing Tiantan Hospital from January 2013 to December 2018. All participants were followed up for at least 3 years, and the development of DRE was assessed. Data from January 2013 to December 2017 were used as development dataset for model building. Those independent predictors of DRE were included in the final multivariable logistic regression, and a derived nomogram was built. Data from January 2018 to December 2018 were used as validation dataset for internal validation.

Results: Complete clinical information was available for 2830 PTE patients (development dataset: 2023; validation dataset: 807), of which 21.06% (n = 596) developed DRE. Among all parameters of interest including gender, age at PTE, family history, severity of traumatic brain injury (TBI), single or multiple injuries, lesion location, post-TBI treatments, acute seizures, PTE latency, seizure type, status epilepticus (SE), and electroencephalogram (EEG) findings, four predictors showed independent effect on DRE, they were age at PTE, seizure type, SE, and EEG findings. A model incorporating these four variables was created, and a nomogram to calculate the probability of DRE using the coefficients of the model was developed. The C-index of the predictive model and the validation was 0.662 and 0.690, respectively. The goodness-of-fit test indicated good calibration for model development and validation (p = 0.272, 0.572).

Conclusions: The proposed nomogram achieved significant potential for clinical utility in the prediction of DRE among PTE patients. The risk of DRE for individual PTE patients can be estimated by using this nomogram, and identified high-risk patients might benefit from non-pharmacological therapies at an early stage.
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http://dx.doi.org/10.1111/cns.13897DOI Listing
July 2022

Transcriptome and Metabolomics Integrated Analysis Reveals Terpene Synthesis Genes Controlling Linalool Synthesis in Grape Berries.

J Agric Food Chem 2022 Jul 12;70(29):9084-9094. Epub 2022 Jul 12.

Institute of Forestry and Pomology, Beijing Academy of Agricultural and Forestry Sciences, Rui-Wang-Fen, Minzhuang Road, Beijing 100093, China.

In this study, four hybrids from the cross between "Italia" and "Tamina" grapes were chosen to investigate their distinct monoterpenoids patterns and candidate genes involved. Monoterpenoid profiles and transcriptome data were generated at four berry developmental stages. Trans-rose oxide, cis-rose oxide, citronellol, neral, nerol, nerol oxide, geraniol, geranial, geranic acid, and cis-isogeraniol were the dominant compounds in R250 hybrid, while linalool, hotrienol, linalool oxide pyranoside, and cis-furan linalool oxide were the main compounds in R77 hybrid. Six TPS-g subfamily genes were found related with the contents of linalool and its related monoterpenoids by weighted gene coexpression network analysis (WGCNA) and phylogenetic analysis. Among them, TPS59 was cloned and functionally verified by transient overexpression in the leaves of . Meanwhile, NAC (), C2C2-GATA (), and bHLH () were selected as candidate transcription factors (TFs) that could regulate the expression of the six TPS-b genes. These data enhanced our understanding on the regulation of monoterpenoid biosynthesis in grapes.
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http://dx.doi.org/10.1021/acs.jafc.2c00368DOI Listing
July 2022

MiR-143-3p Increases the Radiosensitivity of Breast Cancer Cells Through FGF1.

Cell Mol Biol (Noisy-le-grand) 2022 Feb 4;67(5):256-262. Epub 2022 Feb 4.

Department of Pathology, Jingjiang People's Hospital, Jingjiang, 214500, China.

Breast cancer is a common malignant tumor in women. At present, the main treatment for breast cancer is radiotherapy. Due to the difference in radiosensitivity between individuals or tumor cells, the effect of radiotherapy is not good. Therefore, in radiotherapy, how to use various auxiliary means to reduce the radiation resistance of tumor, Therefore, it has become an important research topic to improve the radiosensitivity of the tumor. Fibroblast growth factor-1 (FGF1) plays an important role in tumor migration. Therefore, the study of miR-143-3p increasing the radiosensitivity of breast cancer cells through FGF1 is proposed in this paper. In this study, a control group experiment was set up to study. During the experiment, the relative expression of miR-143-3p was detected by fluorescent quantitative PCR of miRNA, and the cell irradiation experiment was created to analyze the radiosensitivity of breast cancer cells by comparing their survival fraction. The results of this study showed that when the radiation dose was 0, the survival scores of the three groups were all 1. The survival fraction of the experimental group decreased from 0.26 ± 0.045 to 0.068 ± 0.008 when the dose was added to 4Gy. The survival fraction of the experimental group was always greater than that of the two control groups. The results of this study show that miR-143-3p can increase the radiosensitivity of breast cancer cells through FGF1.
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http://dx.doi.org/10.14715/cmb/2021.67.5.35DOI Listing
February 2022

Dietary Effects on Chronic Venous Disease.

Ann Vasc Surg 2022 Jul 8. Epub 2022 Jul 8.

Department of Vascular Surgery, Shanxi Bethune Hospital, Taiyuan, Shanxi, China. Electronic address:

Background: Diet is fundamental to maintaining and improving human health. There is ample evidence identifying the beneficial and/or harmful effects of diet on noncommunicable diseases such as obesity, diabetes mellitus, and cardiovascular disease. However, the associations of the diet to chronic venous disease has not been fully described.

Methods: Data were collected through a cross-sectional survey conducted on 1,571 community-dwelling adults in 2018. Diet intake frequency was assessed using valid food group consumption frequency questionnaires. Multivariable logistic regression models were used to evaluate the association of diet with chronic venous disease.

Results: In total, 857 participants were diagnosed with chronic venous disease. Those who ate soybean products daily and 4-6 days/week had a 51-31% lower risk of chronic venous disease compared with those who only occasionally consumed soybean food, respectively. Participants who consumed eggs and egg products 1-3 days/week versus those who only occasionally ate eggs showed a lower risk of chronic venous disease [odds ratio (OR) 0.542, 95% confidence interval (CI) 0.375-0.782]. Eating fried food 4-6 days each week was associated with an increased risk of chronic venous disease (OR 3.872, 95% CI 1.263-11.599) compared with those who only occasionally ate fried foods. There is a decreasing tendency of the adjusted OR for eating soybean products daily with the severity of disease [chronic venous disease (C0-C2): OR 0.575, 95% CI 0.408-0.812; chronic venous insufficiency (C3-C6): OR 0.222, 95% CI 0.114-0.435].

Conclusions: A higher frequency in the consumption of soybean products and eggs were associated with a lower risk of chronic venous disease. High level of fried food consumption was positively associated with risk of chronic venous disease. There are certain specific trends in relation to dietary consumption and severity of disease, although these trends were less strong. These associations are largely independent of other dietary and nondietary factors.
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http://dx.doi.org/10.1016/j.avsg.2022.06.015DOI Listing
July 2022

Functional Characterization and Structural Basis of the β-1,3-Glucan Synthase CMGLS from Mushroom .

J Agric Food Chem 2022 Jul 11;70(28):8725-8737. Epub 2022 Jul 11.

School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, P.R. China.

β-1,3-Glucan synthases play key roles in glucan synthesis, cell wall assembly, and growth of fungi. However, their multi-transmembrane domains (over 14 TMHs) and large molecular masses (over 100 kDa) significantly hamper understanding of their catalytic characteristics and mechanisms. In the present study, the 5841-bp gene encoding the 221.7 kDa membrane-bound β-1,3-glucan synthase CMGLS in was cloned, identified, and structurally analyzed. CMGLS was partially purified with a specific activity of 87.72 pmol/min/μg, a purification fold of 121, and a yield of 10.16% using a product-entrapment purification method. CMGLS showed a strict specificity to UDP-glucose with a value of 84.28 μM at pH 7.0 and synthesized β-1,3-glucan with a maximum degree of polymerization (DP) of 70. With the assistance of AlphaFold and molecular docking, the 3D structure of CMGLS and its binding features with substrate UDP-glucose were proposed for the first time to our knowledge. UDP-glucose potentially bound to at least 11 residues hydrogen bonds, π-stacking ,and salt bridges, and Arg 1436 was predicted as a key residue directly interacting with the moieties of glucose, phosphate, and the ribose ring on UDP-glucose. These findings would open an avenue to recognize and understand the glucan synthesis process and catalytic mechanism of β-1,3-glucan synthases in mushrooms.
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http://dx.doi.org/10.1021/acs.jafc.2c03410DOI Listing
July 2022

Rapid Identification of Constituents in (Roxb.) Ridsd. et Badh. F. Using UHPLC-Q-Exactive Orbitrap Mass Spectrometry.

Molecules 2022 Jun 23;27(13). Epub 2022 Jun 23.

School of Pharmaceutical Sciences, Hunan University of Medicine, Huaihua 418000, China.

(Roxb.) Ridsd. et Badh. F. (CT) belongs to the Rubiaceae family. Its dried leaves are widely used in traditional Chinese medicine to treat enteritis, dysentery, toothache, furuncles, swelling, traumatic injury, fracture, bleeding, and scalding. In order to further clarify the unknown chemical composition of CT, a rapid strategy based on UHPLC-Q-exactive orbitrap was established for this analysis using a Thermo Scientific Hypersil GOLD aQ (100 mm × 2.1 mm, 1.9 µm) chromatographic column. The mobile phase was 0.1% formic acid water-acetonitrile, with a flow rate of 0.3 mL/min and injection volume of 2 µL; for mass spectrometry, an ESI ion source in positive and negative ion monitoring modes was adopted. A total of 135 chemicals comprising 67 chlorogenic acid derivatives, 48 flavonoids, and 20 anthocyanin derivatives were identified by comparing the mass spectrum information with standard substances, public databases, and the literature, which were all discovered for the first time in this plant. This result broadly expands the chemical composition of CT, which will contribute to understanding of its effectiveness and enable quality control.
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http://dx.doi.org/10.3390/molecules27134038DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9268514PMC
June 2022

Co-assembly Behaviors of Flavonol Derivatives Induced by a Pyridine Derivative on HOPG via Hydrogen Bonding and Van der Waals Forces.

Langmuir 2022 Jul 7;38(28):8651-8656. Epub 2022 Jul 7.

Jiangsu Co-innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, P. R. China.

In this paper, two new flavonol derivatives, 2-(4-(dodecyloxy)phenyl)-3-hydroxyflavone () and 2-(3,5-bis(dodecyloxy)phenyl)-3-hydroxyflavone (), were synthesized to investigate the respective self-assembly behaviors at the liquid/solid interface by scanning tunneling microscopy. In addition, a linear pyridine derivative with acetylene groups called was added to regulate the assembly of and , individually. However, only molecules successfully co-assembled into grid structures with molecules. Furthermore, the assembly and co-assembly behavior mechanism of flavonol derivatives and molecules were further studied by density functional theory calculations. This work will lay a foundation for investigating the self-assembly of flavonol derivatives and the co-assembly regulated by pyridine derivatives at the liquid-solid interface.
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http://dx.doi.org/10.1021/acs.langmuir.2c01076DOI Listing
July 2022

Coherent interfaces govern direct transformation from graphite to diamond.

Nature 2022 07 6;607(7919):486-491. Epub 2022 Jul 6.

Center for High Pressure Science (CHiPS), State Key Laboratory of Metastable Materials Science and Technology, Yanshan University, Qinhuangdao, China.

Understanding the direct transformation from graphite to diamond has been a long-standing challenge with great scientific and practical importance. Previously proposed transformation mechanisms, based on traditional experimental observations that lacked atomistic resolution, cannot account for the complex nanostructures occurring at graphite-diamond interfaces during the transformation. Here we report the identification of coherent graphite-diamond interfaces, which consist of four basic structural motifs, in partially transformed graphite samples recovered from static compression, using high-angle annular dark-field scanning transmission electron microscopy. These observations provide insight into possible pathways of the transformation. Theoretical calculations confirm that transformation through these coherent interfaces is energetically favoured compared with those through other paths previously proposed. The graphite-to-diamond transformation is governed by the formation of nanoscale coherent interfaces (diamond nucleation), which, under static compression, advance to consume the remaining graphite (diamond growth). These results may also shed light on transformation mechanisms of other carbon materials and boron nitride under different synthetic conditions.
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http://dx.doi.org/10.1038/s41586-022-04863-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9300464PMC
July 2022

The role of [email protected] as electron shuttle in enhancing the biodegradation of gaseous para-xylene by aerobic surfactant secreted strains.

J Hazard Mater 2022 Jun 30;438:129475. Epub 2022 Jun 30.

School of Chemical Engineering and Technology, Hainan University, Haikou 570228, China; Key Laboratory of Advanced Materials of Tropical Island Resources, Ministry of Education, Haikou 570228, China; Key Laboratory of Solid Waste Resource Utilization and Environmental Protection of Haikou City, Haikou 570228, China. Electronic address:

To study the role of electron shuttles in accelerating the biodegradation of volatile organic compounds (VOCs) and provide theoretical support for purification of waste gas containing PX, two self-producing biosurfactant strains were used to improve solubility, and the magnetic [email protected] composites were prepared as electron shuttles to accelerate extracellular electron transfer during the process of para-xylene (PX) biodegradation. The composites were characterized by scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, and X-ray photoelectron spectroscopy. The biodegradation time of PX by Enterobacter sp. HN01 and Klebsiella sp. HN02 decreased from 192 h to 12 h and 120 to 12 h, and approximately 93.75% and 90.00% of the removal times were saved after the addition of the composites. Furthermore, the effects of [email protected] on the bacterial biosurfactant secretion, self-enzyme activity, and bacterial growth inhibition by PX were explored. The electron transport capacity of [email protected] was 4.583 mmol·e/g detected by mediated electrochemical reduction and mediated electrochemical oxidation, and possible electron transport pathways were revealed. The possible products of PX biodegradation by HN01 and HN02 were determined through gas chromatography-mass spectrometry. The molecular structure of PX was deduced through density functional theory calculation to validate the key product. Results indicated that [email protected] can be used as an electronic shuttle to accelerate extracellular electron transfer and significantly improve VOCs removal rate.
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http://dx.doi.org/10.1016/j.jhazmat.2022.129475DOI Listing
June 2022
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