Publications by authors named "Lei Shao"

225 Publications

Pre-shear bioprinting of highly oriented porous hydrogel microfibers to construct anisotropic tissues.

Biomater Sci 2021 Jul 21. Epub 2021 Jul 21.

Research Institute for Medical and Biological Engineering, Ningbo University, Ningbo, 315211, China.

Anisotropic tissues in vivo have special structural characteristics and biological functions. Nowadays, bioprinting is widely used in tissue engineering and an effective way to process cell-laden hydrogels. However, the direct bioprinting of oriented cell-laden hydrogel structures to engineer anisotropic tissues is still difficult. Meanwhile, the inherent dense micropore network after the gelation of hydrogel-based bioinks usually limits the normal growth of encapsulated cells due to the inadequate supply of nutrient/oxygen. Herein, we proposed a pre-shear bioprinting strategy of highly oriented porous hydrogel microfibers to construct anisotropic tissues. Firstly, based on the phase separation of viscous high-molecular compound mixtures, we utilized a general viscous porous bioink paradigm, e.g., mixing a polymer thickener (PEO) with a hydrogel precursor (GelMA) with excellent biological properties. Secondly, based on the shear-oriented property of the viscous porous bioink, we designed the pre-shear in situ coaxial bioprinting of highly oriented porous hydrogel microfibers. The viscous porous bioink (GelMA/PEO) was shear-oriented through an injection tube and pumped into the inner needle of a coaxial nozzle. When GelMA/PEO passed through a transparent glass tube connected to the coaxial nozzle, GelMA can be in situ photo-crosslinked to form highly oriented porous microfibers. In addition, we showed the manufacturing of heterogeneous oriented microfibers and the manual assembly of microfibers, and within oriented microfibers, different cells or co-cultured cells exhibited highly oriented growth behaviors similar to that in vivo. As far as we know, the direct bioprinting of anisotropic tissues through high orientation induced by pre-shearing is firstly reported in our study. We believe that the pre-shear bioprinting strategy of anisotropic tissues will open more avenues for further biomedical research.
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http://dx.doi.org/10.1039/d1bm00695aDOI Listing
July 2021

The effects of a multi-disciplinary team on sleep quality assessment in mild-to-moderate Alzheimer's disease patients with sleep disorders.

Scott Med J 2021 Aug 5;66(3):134-141. Epub 2021 Jul 5.

Chief Physician, Department of Psychosomatic Disorders in Huzhou Third Municipal Hospital, The Affiliated Hospital of Huzhou University, China.

Background And Aims: Sleep disturbances are a severe problem among patients with Alzheimer's disease (AD). By evaluating sleep quality in mild-to-moderate AD patients, this study aimed to assess the effects of multi-disciplinary team (MDT) in reducing the incidence of adverse reactions of AD patients. The reduction in the incidence of adverse reactions to predict multi-disciplinary team (MDT) treatment effects.

Methods And Results: This study included 60 mild-to-moderate AD patients with sleep problems when hospitalized in Huzhou Third Municipal Hospital. The patients were randomly distributed into two groups, routine and MDT treatments. The cognitive functions, sleep conditions, and psycho-behavioral symptoms were compared between both the groups. Cognitive function declined significantly between pretherapy and follow-up in the routine treatment group (MMSE: t = -7.961,  < 0.001; MoCA: t = -4.672,  < 0.001). There was a significant decline in drowsiness in the MDT group compared to that in the routine treatment group (χ = 4.320,  = 0.038). Sleep quality improved significantly during the follow-up in the MDT treatment group (t = 6.098,  < 0.001). The results of the Hamilton Depression Scale (HAMD) and Hamilton Anxiety Scale (HAMA) among family caregivers (FCGs) demonstrated that MDT treatment could alleviate caregivers' depression (t = -2.867,  = 0.042), and routine treatment can worsen their anxiety (t = 3.258,  = 0.003).

Conclusion: The MDT treatment method as an effective and meaningful therapy can help mitigate the suffering of patients with AD and FCGs.
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http://dx.doi.org/10.1177/00369330211027450DOI Listing
August 2021

How to Utilize Excited Plasmon Energy Efficiently.

ACS Nano 2021 Jun 17. Epub 2021 Jun 17.

Department of Physics, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, China.

Plasmonic nanoparticles can concentrate electromagnetic fields at the nanoscale and function as a powerful intermediary to enhance light-matter interactions. They have been widely employed for solar energy harvesting, photocatalysis, medicine, sensing, imaging, spectroscopy, optics, and optoelectronics. In this Perspective, we provide a brief overview of research progress in the utilization of excited plasmon energy, with emphasis on the charge- and energy-transfer processes. We discuss important factors that affect the charge- and energy-transfer efficiencies and present open questions and major challenges in the efficient utilization of excited plasmon energy.
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http://dx.doi.org/10.1021/acsnano.1c02627DOI Listing
June 2021

Facet- and Gas-Dependent Reshaping of Au Nanoplates by Plasma Treatment.

ACS Nano 2021 Jun 11;15(6):9860-9870. Epub 2021 Jun 11.

Department of Physics, The Chinese University of Hong Kong, Shatin, Hong Kong SAR China.

The reshaping of metal nanocrystals on substrates is usually realized by pulsed laser irradiation or ion-beam milling with complex procedures. In this work, we demonstrate a simple method for reshaping immobilized Au nanoplates through plasma treatment. Au nanoplates can be reshaped gradually with nearly periodic right pyramid arrays formed on the surface of the nanoplates. The gaseous environment in the plasma-treatment system plays a significant role in the reshaping process with only nitrogen-containing environments leading to reshaping. The reshaping phenomenon is facet-dependent, with right pyramids formed only on the exposed {111} facets of the Au nanoplates. The morphological change of the Au nanoplates induced by the plasma treatment leads to large plasmon peak redshifts. The reshaped Au nanoplates possess slightly higher refractive index sensitivities and largely increased surface-enhanced Raman scattering intensities compared to the flat, untreated nanoplates. Our results offer insights for studying the interaction mechanism between plasma and the different facets of noble metal nanocrystals and an approach for reshaping light-interacting noble metal nanocrystals.
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http://dx.doi.org/10.1021/acsnano.1c00861DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8223482PMC
June 2021

The seborrheic dermatitis-distributed dermatomyositis: A facial cutaneous lesions sign associated with positive anti-MDA5 antibody.

J Cosmet Dermatol 2021 May 8. Epub 2021 May 8.

Department of Allergy, Zibo Central Hospital, Zibo, China.

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http://dx.doi.org/10.1111/jocd.14204DOI Listing
May 2021

Selective Deposition of Catalytic Metals on Plasmonic Au Nanocups for Room-Light-Active Photooxidation of -Phenylenediamine.

ACS Appl Mater Interfaces 2021 Apr 28. Epub 2021 Apr 28.

Department of Physics, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, China.

Plasmonic hotspots can enhance hot charge carrier generation, offering new opportunities for improving the photocatalytic activity. In this work, eight types of heteronanostructures are synthesized by selectively depositing catalytic metals at the different sites of highly asymmetric Au nanocups for the photocatalytic oxidation of -phenylenediamine. The oxidation of this molecule has so far mainly relied on the use of HO as an oxidizing agent in the presence of an appropriate catalyst. The photocatalytic oxidation under visible light has not been reported before. The Au nanocups with AgPt nanoparticles grown at the opening edge and bottom exhibit the highest photocatalytic activity. The generated hot electrons and holes both participate in the reaction. The hot carriers from the interband and intraband transitions are both utilized. The optimal catalyst shows a favorable activity even under room light. Simulations reveal that the profound electric field enhancement at the hotspots boosts the hot-carrier density in the catalytic nanoparticles, explaining the overwhelming photocatalytic activity of the optimal catalyst.
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http://dx.doi.org/10.1021/acsami.1c03806DOI Listing
April 2021

Deep Learning-Based Estimation of Axial Length and Subfoveal Choroidal Thickness From Color Fundus Photographs.

Front Cell Dev Biol 2021 9;9:653692. Epub 2021 Apr 9.

Beijing Key Laboratory of Intraocular Tumor Diagnosis and Treatment, Beijing Ophthalmology and Visual Sciences Key Laboratory, Medical Artificial Intelligence Research and Verification Key Laboratory of the Ministry of Industry and Information Technology, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing, China.

This study aimed to develop an automated computer-based algorithm to estimate axial length and subfoveal choroidal thickness (SFCT) based on color fundus photographs. In the population-based Beijing Eye Study 2011, we took fundus photographs and measured SFCT by optical coherence tomography (OCT) and axial length by optical low-coherence reflectometry. Using 6394 color fundus images taken from 3468 participants, we trained and evaluated a deep-learning-based algorithm for estimation of axial length and SFCT. The algorithm had a mean absolute error (MAE) for estimating axial length and SFCT of 0.56 mm [95% confidence interval (CI): 0.53,0.61] and 49.20 μm (95% CI: 45.83,52.54), respectively. Estimated values and measured data showed coefficients of determination of = 0.59 (95% CI: 0.50,0.65) for axial length and = 0.62 (95% CI: 0.57,0.67) for SFCT. Bland-Altman plots revealed a mean difference in axial length and SFCT of -0.16 mm (95% CI: -1.60,1.27 mm) and of -4.40 μm (95% CI, -131.8,122.9 μm), respectively. For the estimation of axial length, heat map analysis showed that signals predominantly from overall of the macular region, the foveal region, and the extrafoveal region were used in the eyes with an axial length of < 22 mm, 22-26 mm, and > 26 mm, respectively. For the estimation of SFCT, the convolutional neural network (CNN) used mostly the central part of the macular region, the fovea or perifovea, independently of the SFCT. Our study shows that deep-learning-based algorithms may be helpful in estimating axial length and SFCT based on conventional color fundus images. They may be a further step in the semiautomatic assessment of the eye.
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http://dx.doi.org/10.3389/fcell.2021.653692DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8063031PMC
April 2021

Control of light-valley interactions in 2D transition metal dichalcogenides with nanophotonic structures.

Nanoscale 2021 Apr 25;13(13):6357-6372. Epub 2021 Mar 25.

Beijing Computational Science Research Center, Beijing 100193, China.

Electronic valley in two-dimensional transition-metal dichalcogenides (2D TMDCs) offers a new degree of freedom for information storage and processing. The valley pseudospin can be optically encoded by photons with specific helicity, enabling the construction of electronic information devices with both high performance and low power consumption. Robust detection, manipulation and transport of the valley pseudospins at room temperature are still challenging because of the short lifetime of valley-polarized carriers and excitons. Integrating 2D TMDCs with nanophotonic objects such as plasmonic nanostructures provides a competitive solution to address the challenge. The research in this field is of practical interest and can also present rich physics of light-matter interactions. In this minireview, recent progress on using nanophotonic strategies to enhance the valley polarization degree, especially at room temperature, is highlighted. Open questions, major challenges, and interesting future developments in manipulating the valley information in 2D semiconductors with the help of nanophotonic structures will also be discussed.
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http://dx.doi.org/10.1039/d0nr08000dDOI Listing
April 2021

RNA-Seq Identifies Marked Th17 Cell Activation and Altered CFTR Expression in Different Atopic Dermatitis Subtypes in Chinese Han Populations.

Front Immunol 2021 1;12:628512. Epub 2021 Apr 1.

Department of Dermatology, Guangzhou Institute of Dermatology, Guangzhou, China.

Background: Patients with atopic dermatitis (AD) exhibit phenotypic variability in ethnicity and IgE status. In addition, some patients develop other allergic conditions, such as allergic rhinitis (AR), in subsequent life. Understanding the heterogeneity of AD would be beneficial to phenotype-specific therapies.

Methods: Twenty-eight Chinese AD patients and 8 healthy volunteers were enrolled in the study. High-throughput transcriptome sequencing was conducted on lesional and nonlesional skin samples from 10 AD patients and matched normal skin samples from 5 healthy volunteers. Identification of differentially expressed genes (DEGs), KEGG pathway analyses, and sample cluster analyses were conducted in the R software environment using the DEseq2, ClusterProfiler, and pheatmap R packages, respectively. qRT-PCR, Western blotting, and ELISA were used to detect gene expression levels among subtypes. Correlation analysis was performed to further investigate their correlation with disease severity.

Results: A total of 25,798 genes were detected per sample. Subgroup differential expression analysis and functional enrichment analysis revealed significant changes in the IL17 signaling pathway in Chinese EAD patients but not in IAD patients. DEGs enriched in cytokine-cytokine receptor interactions and gland secretion were considered to be associated with atopic march. Further investigations confirmed a marked IL17A upregulation in Chinese EAD with a positive relationship with total IgE level and AD severity. In addition, increased IL17A in AD patients with AR demonstrated a closer association with AR severity than IL4R. Moreover, AQP5 and CFTR were decreased in the lesions of AD patients with AR. The CFTR mRNA expression level was negatively associated with the skin IL17A level and AR severity.

Conclusion: Our research characterized marked Th17 activation in Chinese EAD patients, and altered expression of IL17A, IL4R, AQP5, and CFTR in AD patients with AR was associated with AR severity. It partially explained the phenotypic differences of AD subtypes and provided potential references for endotype-targeted therapy.
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http://dx.doi.org/10.3389/fimmu.2021.628512DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8047326PMC
April 2021

The relationship between Subfoveal Choroidal Thickness and Hypertensive Retinopathy.

Sci Rep 2021 Mar 9;11(1):5460. Epub 2021 Mar 9.

Beijing Tongren Eye Center, Beijing Key Laboratory of Intraocular Tumor Diagnosis and Treatment, Beijing Tongren Hospital, Capital Medical University, 1 Dong Jiao Min Xiang, Dong Cheng District, Beijing, 100730, China.

The Beijing Eye Study 2011 is a population-based cross-sectional study in Northern China, which enrolled 3468 participants whose age were more than 50 years. A detailed ophthalmic examination was performed including spectral-domain optical coherence tomography with enhanced depth imaging for measurement of SFCT and fundus photography. Blood pressure, fundus photographs and choroidal OCT-images were available for 3237 (93.3%) subjects, with 1953 (56.3 ± 0.8%) of the study population fulfilled the diagnosis of hypertension and 1089 subjects having hypertensive retinopathy. For the hypertensive cases, the SFCT in patients with hypertensive retinopathy (286.48 ± 105.23 µm) was significantly thicker than subjects without hypertensive retinopathy (187.04 ± 78.80 µm, P < 0.001). SFCT was significantly associated with the stage of hypertensive retinopathy (P < 0.001), but not significantly associated with diastolic blood pressure (P = 0.94), history (P = 0.95) and years (P = 0.91) of hypertension. In conclusion, hypertension as systemic disease was not significantly affect the subfoveal choroidal thickness, but as ocular disease, hypertensive retinopathy was significantly related to changes of choroidal thickness. Lesions of choroid during chronic hypertension may play an important role in development of hypertensive retinopathy.
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http://dx.doi.org/10.1038/s41598-021-84947-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7943765PMC
March 2021

Deletion at an 1q24 locus reveals a critical role of long noncoding RNA DNM3OS in skeletal development.

Cell Biosci 2021 Mar 2;11(1):47. Epub 2021 Mar 2.

Department of Medical Genetics, School of Basic Medical Sciences, Nanjing Medical University, Jiangsu, 211166, Nanjing, P. R. China.

Background: Skeletal development and maintenance are complex processes known to be coordinated by multiple genetic and epigenetic signaling pathways. However, the role of long non-coding RNAs (lncRNAs), a class of crucial epigenetic regulatory molecules, has been under explored in skeletal biology.

Results: Here we report a young patient with short stature, hypothalamic dysfunction and mild macrocephaly, who carries a maternally inherited 690 kb deletion at Chr.1q24.2 encompassing a noncoding RNA gene, DNM3OS, embedded on the opposite strand in an intron of the DYNAMIN 3 (DNM3) gene. We show that lncRNA DNM3OS sustains the proliferation of chondrocytes independent of two co-cistronic microRNAs miR-199a and miR-214. We further show that nerve growth factor (NGF), a known factor of chondrocyte growth, is a key target of DNM3OS-mediated control of chondrocyte proliferation.

Conclusions: This work demonstrates that DNM3OS is essential for preventing premature differentiation of chondrocytes required for bone growth through endochondral ossification.
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http://dx.doi.org/10.1186/s13578-021-00559-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7923828PMC
March 2021

Construction of Zn/Ni Bimetallic Organic Framework Derived ZnO/NiO Heterostructure with Superior -Propanol Sensing Performance.

ACS Appl Mater Interfaces 2021 Feb 8;13(7):9206-9215. Epub 2021 Feb 8.

Department of Chemistry, College of Sciences, Northeastern University, Shenyang 110819, China.

Bimetallic organic frameworks (Bi-MOFs) have been recognized as one of the most ideal precursors to construct metal oxide semiconductor (MOS) composites, owing to their high surface area, various chemical structures, and easy removal of the sacrificial MOF scaffolds through calcination. Herein, we synthesized Zn/Ni Bi-MOF for the first time via a facile ion exchange postsynthetic strategy, formed a three-dimensional framework consisting of infinite one-dimensional chains that is unattainable through the direct solvothermal approach, and then transformed the Zn/Ni Bi-MOF into a unique ZnO/NiO heterostructure through calcination. Notably, the obtained sensor based on a ZnO/NiO heterostructure exhibits an ultrahigh response of 280.2 toward 500 ppm -propanol at 275 °C (17.2-fold enhancement compared with that of ZnO), remarkable selectivity, and a limit of detection of 200 ppb with a notable response (2.51), which outperforms state-of-the-art -propanol sensors. The enhanced -propanol sensing properties may be attributed to the synergistic effects of several points including the heterojunction at the interface between the NiO and ZnO nanoparticles, especially a one-dimensional chain MOF template structure as well as the chemical sensitization effect of NiO. This work provides a promising strategy for the development of a novel Bi-MOF-derived MOS heterostructure or homostructure with well-defined morphology and composition that can be applied to the fields of gas sensing, energy storage, and catalysis.
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http://dx.doi.org/10.1021/acsami.0c21583DOI Listing
February 2021

Degradation of Bisphenol A by ozonation in rotating packed bed: Effects of operational parameters and co-existing chemicals.

Chemosphere 2021 Jul 26;274:129769. Epub 2021 Jan 26.

Research Center of the Ministry of Education for High Gravity Engineering and Technology, Beijing University of Chemical Technology, Beijing, 100029, China. Electronic address:

Bisphenol A (BPA), a typical endocrine disrupting chemical, widely exists in water and threatens human health. The degradation of BPA by ozone in water is limited by the gas-mass transfer due to the low solubility of ozone. In this study, a rotating packed bed (RPB) was employed to create a high gravity environment to intensify the ozone mass transfer and BPA degradation. The effects of operational parameters (rotation speed of RPB, pH of the solution, ozone concentration, BPA concentration, gas volumetric flow rate and liquid volumetric flow rate) on BPA degradation efficiency and overall volumetric mass transfer coefficient of ozone were investigated. The results show that RPB effectively promoted the ozone mass transfer and BPA degradation and can be used for the ozonation of micropollutants that have fast reaction rates with ozone. Quenching experiments suggest that both ozone and HO∙ participated in BPA degradation from acidic to alkaline environments. In addition, the effects of co-existing chemicals on BPA degradation efficiency were studied. The addition of HO or Cl had no obvious impact on BPA degradation; the addition of HCO is beneficial for BPA degradation while the addition of fulvic acid suppressed the degradation. These results indicate that the pH value, which affects the reaction rate between ozone and BPA, is a major factor to be considered during the ozonation of BPA in RPB.
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http://dx.doi.org/10.1016/j.chemosphere.2021.129769DOI Listing
July 2021

The Low-Alkalinity Polymyxin Derivative, AL-6, Shows High Activity Against Multidrug-Resistant Clinical Isolates and ATCC 19606 : Preliminary Analysis of the Antibacterial Mechanism.

Microb Drug Resist 2021 Jul 5;27(7):933-941. Epub 2021 Feb 5.

Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Polymyxin B and colistin (polymyxin E) are increasingly used as the last line of therapy for infections caused by multidrug-resistant (MDR) gram-negative pathogens. However, nephrotoxicity is still a limiting factor for the use of polymyxin. Therefore, better tolerated and more effective polymyxin derivatives are urgently needed. In this study, we aimed to evaluate the activity of the low-alkalinity polymyxin derivative, AL-6, against MDR (Ab) clinical isolates and ATCC 19606 . Additionally, we performed a preliminarily study of the antibacterial mechanism. AL-6 showed much higher activity (0.125-0.25 μg/mL) against MDR clinical isolates than polymyxin E (PE, 0.5-1 μg/mL). AL-6 also showed much higher activity (0.5-256 μg/mL) against polymyxin-resistant strains than PE (16-1024 μg/mL). Additionally, AL-6 showed slow resistance against . AL-6 also increased the survival rates of mice by 10% at 48 h compared with PE (5 mg/kg). AL-6 could be used at a dose of up to 10 mg/kg, increasing the survival rate to 30% at 72 h after infection. A preliminary study of the antibacterial mechanism showed that AL-6 permeabilized the outer membrane and destroyed cell membrane integrity. Moreover, there was a substantial increase in zeta potential (., less negative) upon AL-6 exposure for . Overall, AL-6 carrying only four positive charges showed high activity against by disrupting cell membrane integrity. Higher doses of AL-6 could increase survival rates of mice. Thus, AL-6 may have potential applications as a bactericidal agent.
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http://dx.doi.org/10.1089/mdr.2019.0474DOI Listing
July 2021

The complete chloroplast genome of (Orchidaceae) and phylogenetic analysis.

Mitochondrial DNA B Resour 2020 Jul 23;5(3):2930-2931. Epub 2020 Jul 23.

College of Landscape Architecture, Beijing Forestry University, Beijing, China.

is a common cultivated species in the genus due to its elegant appearance, rich flower colors and strong fragrance, but its classification is quite controversial. In this study, the complete chloroplast genome of was obtained by Illumina sequencing. The chloroplast genome of is 150,070 bp in length with an overall GC content of 37.12%, which contains a large single-copy (LSC;84,949 bp) region, a small single-copy (SSC;13,745bp) region, and a pair of inverted repeats (IRs; 25,688 bp) regions. The genome contains 130 genes, namely 84 protein-coding genes, 38 tRNA genes and 8 rRNA genes. The maximum-likelihood phylogenetic tree has proved that should exist as an independent species in the genus , and it is most closely related to . This study provides valuable sequence resources for further study of .
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http://dx.doi.org/10.1080/23802359.2020.1780968DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7782654PMC
July 2020

The complete chloroplast genome of (Orchidaceae): a rare and Endangered species endemic to Southwest China.

Mitochondrial DNA B Resour 2020 Jun 11;5(3):2429-2431. Epub 2020 Jun 11.

Zhejiang Provincial Key Laboratory of Germplasm Innovation and Utilization for Garden Plants, Key Laboratory of National Forestry and Grassland Administration on Germplasm Innovation and Utilization for Southern Garden Plants, School of Landscape Architecture, Zhejiang Agriculture and Forestry University, Hangzhou, China.

is a dominant species in the large orchid family with beautiful flowers, thick petals, and long flowering periods, and has a long history of cultivation in Southwest China. However, its wild resources have been threatened with extinction due to environmental degradation and artificial exploitation. In this study, the complete chloroplast genome of was obtained through Illumina sequencing. The size of chloroplast genome of is 149,998 bp, including large single-copy (LSC) and small single-copy (SSC) regions over 84,854 bp and 13,926 bp, respectively, and two inverted repeats (IRs) of 25,609 bp. The total GC content was 37.11%. The chloroplast genome contains 129 genes, including 83 protein-coding genes, 8 rRNA genes, and 38 tRNA genes. The maximum-likelihood phylogenetic tree indicated that is a sister species with the clade composed of and . The complete chloroplast genome of will contribute to protecting this highly endangered species, and provide genetic information about genetic diversity and sustainable use.
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http://dx.doi.org/10.1080/23802359.2020.1775514DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7782322PMC
June 2020

Effects of security on social trust among Chinese adults: Roles of life satisfaction and ostracism.

J Soc Psychol 2021 Jan 7:1-10. Epub 2021 Jan 7.

Renmin University of China.

The study aims to explore the effect of security on social trust and the roles of life satisfaction and ostracism among Chinese adults. Toward this end, the study recruited 1,206 Chinese adults to complete the Security Questionnaire, Social Trust Questionnaire, Satisfaction with Life Scale, and Ostracism Experiences Scale. Results show that (a) security is positively correlated with social trust among Chinese adults, (b) life satisfaction partially mediates the effect of security on social trust, and (c) ostracism moderates the relationship between security and social trust. Furthermore, findings indicate that security can directly predict social trust as well as indirectly predict social trust through life satisfaction for adults in China. Security has a strong predictive effect on social trust - especially for individuals with low levels of ostracism.
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http://dx.doi.org/10.1080/00224545.2020.1871312DOI Listing
January 2021

Corrigendum to "Special Staining of the Liquid-Based Cytopathology Test in Bronchoalveolar Lavage Fluid for Diagnosis of Invasive Pulmonary Aspergillosis with Nonneutropenic Patients".

Can Respir J 2020 6;2020:7893513. Epub 2020 Nov 6.

Department of Pulmonary and Critical Care Medicine, The Affiliated Hospital of Medical School of Ningbo University, 247 Renmin Road, Ningbo, Zhejiang 315020, China.

[This corrects the article DOI: 10.1155/2020/8243473.].
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http://dx.doi.org/10.1155/2020/7893513DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7669353PMC
November 2020

Gut Microbiota Approach-A New Strategy to Treat Parkinson's Disease.

Front Cell Infect Microbiol 2020 22;10:570658. Epub 2020 Oct 22.

Microbial Pharmacology Laboratory, Shanghai University of Medicine & Health Sciences, Shanghai, China.

Parkinson's disease (PD) is a progressive neurodegenerative disorder characterized by neuronal loss and dysfunction of dopaminergic neurons located in the substantia nigra, which contain a variety of misfolded α-synuclein (α-syn). Medications that increase or substitute for dopamine can be used for the treatment of PD. Recently, numerous studies have shown gut microbiota plays a crucial role in regulating and maintaining multiple aspects of host physiology including host metabolism and neurodevelopment. In this review article, the role of gut microbiota in the etiological mechanism of PD will be reviewed. Furthermore, we discussed current pharmaceutical medicine-based methods to prevent and treat PD, followed by describing specific strains that affect the host brain function through the gut-brain axis. We explained in detail how gut microbiota directly produces neurotransmitters or regulate the host biosynthesis of neurotransmitters. The neurotransmitters secreted by the intestinal lumen bacteria may induce epithelial cells to release molecules that, in turn, can regulate neural signaling in the enteric nervous system and subsequently control brain function and behavior through the brain-gut axis. Finally, we proved that the microbial regulation of the host neuronal system. Endogenous α-syn can be transmitted long distance and bidirectional between ENS and brain through the circulatory system which gives us a new option that the possibility of altering the community of gut microbiota in completely new medication option for treating PD.
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http://dx.doi.org/10.3389/fcimb.2020.570658DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7643014PMC
June 2021

Optimal quantum phase estimation in an atomic gyroscope based on a Bose-Hubbard model.

Opt Express 2020 Oct;28(22):32556-32571

We investigate the optimal quantum state for an atomic gyroscope based on a three-site Bose-Hubbard model. In previous studies, various states such as the uncorrelated state, the BAT state and the NOON state are employed as the probe states to estimate the phase uncertainty. In this article, we present a Hermitian operator and an equivalent unitary parametrization transformation to calculate the quantum Fisher information for any initial states. Exploiting this equivalent unitary parametrization transformation, we can seek the optimal state that gives the maximal quantum Fisher information on both lossless and lossy conditions. As a result, we find that the squeezed entangled state (SES) and the entangled even squeezed state (EESS) can significantly enhance the precision for moderate loss rates compared with previous proposals.
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http://dx.doi.org/10.1364/OE.403156DOI Listing
October 2020

(Gold nanorod core)/(poly(3,4-ethylene-dioxythiophene) shell) nanostructures and their monolayer arrays for plasmonic switching.

Nanoscale 2020 Oct;12(40):20684-20692

College of Chemical Engineering, Huaqiao University, Xiamen 361021, China. and Fujian Provincial Key Laboratory of Biochemical Technology, Huaqiao University, Xiamen 361021, China.

(Gold nanorod core)/(poly(3,4-ethylene-dioxythiophene) (PEDOT) shell) nanostructures are prepared by the surfactant-assisted oxidative polymerization of 3,4-ethylene-dioxythiophene on the surface of gold nanorods (NRs). The PEDOT shell exhibits distinct dielectric properties at doped and undoped states, which allows the manipulation of plasmonic responses of the Au nanorod core. The shift in plasmon resonance induced by the dedoping of PEDOT is found to be associated with the overlap between the plasmon resonance band of the core/shell nanostructure and the spectral region where the largest refractive index variation of PEDOT occurs, as well as with the type of the dedopant. Macroscopic two-dimensional (2D) monolayer arrays of core/shell nanostructures with controlled particle number densities are fabricated on indium tin oxide (ITO)-coated glass substrates by electrophoretic deposition. A reversible plasmonic shift of about 70 nm is obtained on the core/shell nanostructure monolayer array with a number density of around 18 particles per μm2. Our design of colloidal (Au nanorod core)/(PEDOT shell) nanostructures and their 2D monolayer arrays paves the way for the fabrication of high-performance plasmonic switches in large-scale practical usages as well as for the preparation of advanced, programmable chromic materials for a broad range of applications, such as smart windows, anti-counterfeiting tags, and medical and environmental sensors.
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http://dx.doi.org/10.1039/d0nr05502fDOI Listing
October 2020

CD248 as a novel therapeutic target in pulmonary arterial hypertension.

Clin Transl Med 2020 Sep;10(5):e175

Department of Physiology, Jinzhou Medical University, Jinzhou, P. R. China.

Pulmonary vascular remodeling is the most important pathological characteristic of pulmonary arterial hypertension (PAH). No effective treatment for PAH is currently available because the mechanism underlying vascular remodeling is not completely clear. CD248, also known as endosialin, is a transmembrane protein that is highly expressed in pericytes and fibroblasts. Here, we evaluated the role of CD248 in pulmonary vascular remodeling and the processes of PAH pathogenesis. Activation of CD248 in pulmonary artery smooth muscle cells (PASMCs) was found to be proportional to the severity of PAH. CD248 contributed to platelet-derived growth factor-BB (PDGF-BB)-induced PASMC proliferation and migration along with the shift to more synthetic phenotypes. In contrast, treatment with Cd248 siRNA or the anti-CD248 therapeutic antibody (ontuxizumab) significantly inhibited the PDGF signaling pathway, obstructed NF-κB p65-mediated transcription of Nox4, and decreased reactive oxygen species production induced by PDGF-BB in PAMSCs. In addition, knockdown of CD248 alleviated pulmonary vascular remodeling in rat PAH models. This study provides novel insights into the dysfunction of PASMCs leading to pulmonary vascular remodeling, and provides evidence for anti-remodeling treatment for PAH via the immediate targeting of CD248.
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http://dx.doi.org/10.1002/ctm2.175DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7507048PMC
September 2020

Preparation of MOF Film/Aerogel Composite Catalysts via Substrate-Seeding Secondary-Growth for the Oxygen Evolution Reaction and CO Cycloaddition.

Angew Chem Int Ed Engl 2021 Jan 9;60(2):701-705. Epub 2020 Nov 9.

Institute of Metal Research, Shenyang National Laboratory for Materials Science, Chinese Academy of Sciences, Shenyang, 110016, P. R. China.

Substrate-supported metal-organic frameworks (MOFs) films are desired to realize their potential in practical applications. Herein, a novel substrate-seeding secondary-growth strategy is developed to prepare composites of uniform MOFs films on aerogel walls. Briefly, the organic ligand is "pre-seeded" onto the aerogel walls, and then a small amount of metal-ion solution is sprayed onto the prepared aerogel. The sprayed solution diffuses along the aerogel walls to form a continuous thin layer, which confines the nucleation reaction, promoting the formation of uniform MOFs films on the aerogel walls. The whole process is simple in operation, highly efficient, and eco-friendly. The resulting hierarchical MOFs/aerogel composites have abundant accessible active sites and enable excellent mass transfer, which endows the composite with outstanding catalytic activity and stability in both liquid-phase CO cycloaddition and electrochemical oxygen evolution reaction (OER) process.
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http://dx.doi.org/10.1002/anie.202012354DOI Listing
January 2021

Room-temperature valleytronic transistor.

Nat Nanotechnol 2020 Sep 20;15(9):743-749. Epub 2020 Jul 20.

School of Electronic Science and Engineering, Nanjing University, Nanjing, China.

Valleytronics, based on the valley degree of freedom rather than charge, is a promising candidate for next-generation information devices beyond complementary metal-oxide-semiconductor (CMOS) technology. Although many intriguing valleytronic properties have been explored based on excitonic injection or the non-local response of transverse current schemes at low temperature, demonstrations of valleytronic building blocks similar to transistors in electronics, especially at room temperature, remain elusive. Here, we report a solid-state device that enables a full sequence of generating, propagating, detecting and manipulating valley information at room temperature. Chiral nanocrescent plasmonic antennae are used to selectively generate valley-polarized carriers in MoS through hot-electron injection under linearly polarized infrared excitation. These long-lived valley-polarized free carriers can be detected in a valley Hall configuration even without charge current, and can propagate over 18 μm by means of drift. In addition, electrostatic gating allows us to modulate the magnitude of the valley Hall voltage. The electrical valley Hall output could drive the valley manipulation of a cascaded stage, rendering the device able to serve as a transistor free of charge current with pure valleytronic input/output. Our results demonstrate the possibility of encoding and processing information by valley degree of freedom, and provide a universal strategy to study the Berry curvature dipole in quantum materials.
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http://dx.doi.org/10.1038/s41565-020-0727-0DOI Listing
September 2020

Pathogenic characteristics of a QX-like infectious bronchitis virus strain SD in chickens exposed at different ages and protective efficacy of combining live homologous and heterologous vaccination.

Vet Res 2020 Jul 8;51(1):86. Epub 2020 Jul 8.

Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, No. 2 Yuanmingyuan West Road, Haidian, Beijing, 100193, China.

Continued reports of infections with infectious bronchitis virus (IBV) variants have occurred since its first isolation in the 1930s. Currently, QX-like IBVs are the predominant circulating genotype around the world. Here, the pathogenicity of QX-like IBV strain SD was characterized in chickens at different ages of exposure to the virus, and the protection efficacy of available vaccine combinations against IBV was evaluated. The results revealed that QX-like IBV strain SD was severely pathogenic in chickens, causing respiratory, urinary and reproductive infections, irrespective of age, based on clinical observations, viral distribution in tissues and a ciliostasis study. Severe respiratory signs, tracheal cilia injury, nephritis and abnormal development of the oviduct and ovarian follicles were evident throughout the experiment. A challenge experiment demonstrated that the homologous QX vaccine showed superior protection efficacy compared with other available vaccines, confirming the importance of IBV vaccine seed homology against the circulating IBV strains. Our findings aid an understanding of the pathogenicity of QX-like IBVs that may help to further control the infection.
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http://dx.doi.org/10.1186/s13567-020-00811-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7341716PMC
July 2020

EEG-Controlled Wall-Crawling Cleaning Robot Using SSVEP-Based Brain-Computer Interface.

J Healthc Eng 2020 11;2020:6968713. Epub 2020 Jan 11.

Key Laboratory for Control Theory & Applications in Complicated Systems, Tianjin University of Technology, Tianjin 300384, China.

The assistive, adaptive, and rehabilitative applications of EEG-based robot control and navigation are undergoing a major transformation in dimension as well as scope. Under the background of artificial intelligence, medical and nonmedical robots have rapidly developed and have gradually been applied to enhance the quality of people's lives. We focus on connecting the brain with a mobile home robot by translating brain signals to computer commands to build a brain-computer interface that may offer the promise of greatly enhancing the quality of life of disabled and able-bodied people by considerably improving their autonomy, mobility, and abilities. Several types of robots have been controlled using BCI systems to complete real-time simple and/or complicated tasks with high performances. In this paper, a new EEG-based intelligent teleoperation system was designed for a mobile wall-crawling cleaning robot. This robot uses crawler type instead of the traditional wheel type to be used for window or floor cleaning. For EEG-based system controlling the robot position to climb the wall and complete the tasks of cleaning, we extracted steady state visually evoked potential (SSVEP) from the collected electroencephalography (EEG) signal. The visual stimulation interface in the proposed SSVEP-based BCI was composed of four flicker pieces with different frequencies (e.g., 6 Hz, 7.5 Hz, 8.57 Hz, and 10 Hz). Seven subjects were able to smoothly control the movement directions of the cleaning robot by looking at the corresponding flicker using their brain activity. To solve the multiclass problem, thereby achieving the purpose of cleaning the wall within a short period, the canonical correlation analysis (CCA) classification algorithm had been used. Offline and online experiments were held to analyze/classify EEG signals and use them as real-time commands. The proposed system was efficient in the classification and control phases with an obtained accuracy of 89.92% and had an efficient response speed and timing with a bit rate of 22.23 bits/min. These results suggested that the proposed EEG-based clean robot system is promising for smart home control in terms of completing the tasks of cleaning the walls with efficiency, safety, and robustness.
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http://dx.doi.org/10.1155/2020/6968713DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7201509PMC
May 2021

Special Staining of the Liquid-Based Cytopathology Test in Bronchoalveolar Lavage Fluid for Diagnosis of Invasive Pulmonary Aspergillosis with Nonneutropenic Patients.

Can Respir J 2020 31;2020:8243473. Epub 2020 Mar 31.

Department of Pulmonary and Critical Care Medicine, The Affiliated Hospital of Medical School of Ningbo University, 247 Renmin Road, Ningbo, Zhejiang 315020, China.

In recent years, various biomarkers have been gradually applied on bronchoalveolar lavage (BAL) fluid for the diagnosis of invasive pulmonary aspergillosis (IPA). The objective of this study is to assess the value of the liquid-based cytopathology test (LCT) for improving the identification of IPA in BAL fluid from possible IPA patients, following special staining with periodic acid-Schiff staining (PAS) or Grocott's methenamine silver (GMS). A total of 47 consecutive possible IPA patients who underwent bronchoscopy with BAL fluid from January 2017 to December 2018 were included. 45 people had a pair of BAL fluid specimens and 2 patients had two BAL fluid specimens. The 49 pairs of BAL fluid specimens were processed for culture, tuberculosis acid fast staining smear, direct microbial smear, and LCT with special staining (PAS and GMS), respectively. Then, we compared the sensitivity and specificity of PAS and GMS in BAL fluid in high-risk patients. Among 47 possible IPA patients, 25 patients had proven/probable IPA, and 11 patients had other invasive fungal diseases. The sensitivity of GMS was higher than that of PAS (92.11% versus 81.58%; = 0.175). The specificity of GMS was 81.82%, which was higher than that of PAS (81.82% versus 72.73%; = 0.611). The negative predictive value (NPV) for PAS and GMS were 53.33% and 75.00%, respectively. The positive predictive value (PPV) for PAS and GMS were 91.18% and 94.59%, respectively. This study showed that special staining of LCT in BAL fluid may be a novel method for the diagnosis of IPA, and the GMS of LCT had higher sensitivity and specificity, which was superior to PAS.
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http://dx.doi.org/10.1155/2020/8243473DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7150679PMC
June 2021

New Mutations Involved in Colistin Resistance in Acinetobacter baumannii.

mSphere 2020 04 1;5(2). Epub 2020 Apr 1.

School of Pharmacy, Shanghai Jiao Tong University, Shanghai, China

Colistin is used as the "last resort" to treat infections caused by multidrug-resistant , which is at the top of the World Health Organization's list of the most dangerous bacterial species that threaten human health. Unfortunately, colistin resistance has emerged in To broaden the study of the resistance mechanism of colistin in , we obtained colistin-resistant mutants via two methods: (i) screening and isolation from a -based ATCC 19606 transposon mutant library; (ii) selection from challenge of ATCC 19606 with successively increasing concentrations of colistin. A total of 41 mutants with colistin MIC of 4 μg/ml to 64 μg/ml were obtained by transposon mutant library screening. Five highly resistant mutants with colistin MICs ranging from 256 μg/ml to 512 μg/ml were selected from successive colistin challenges. Genotypic complementation and remodeling of the transposon mutants revealed that the genes inactivated by the transposon insertion were not responsible for resistance. Whole-genome sequence analysis of the colistin-resistant strains revealed that the main causes of the resistance to colistin were mutations in the genes, including , , and and the novel alleles and Interestingly, we found that mutation of strain ATCC 19606 ( ) resulted in 4-fold increases in the colistin MIC, which rose from 32 μg/ml to 128 μg/ml. But itself had little effect on the colistin susceptibility of ATCC 19606. These data broaden knowledge of the scope of chromosomally encoded mechanisms of resistance to colistin. is an important Gram-negative opportunistic pathogen commonly infecting critically ill patients. It possesses a remarkable ability to survive in the hospital environment and acquires resistance determinants corresponding to a wide range of antibacterial agents. Given that the current treatment options for multidrug resistant are extremely limited, colistin administration has become the treatment of last resort. However, colistin-resistant strains have recently been reported. The mechanism of resistance to colistin in has rarely been reported. Here, we found two novel mutations in (I13M) and (Q270P) that caused colistin resistance. It is also first reported here that the presence of with a I221V mutation enhanced the colistin resistance of .
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http://dx.doi.org/10.1128/mSphere.00895-19DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7113586PMC
April 2020

New azaphilones, phomopsones A-C with biological activities from an endophytic fungus Phomopsis sp. CGMCC No.5416.

Fitoterapia 2020 Sep 25;145:104573. Epub 2020 Mar 25.

Microbial Pharmacology Laboratory, Shanghai University of Medine&Health Sciences, Pudong, Shanghai 201318, China. Electronic address:

Three undescribed azaphilones, phomopsones A-C (1-3) and two known azaphilones (4-5) were isolated from the culture of endophytic fungus Phomopsis sp. CGMCC No.5416 from the stems of Achyranthes bidentata. Their structures were determined by spectroscopic analysis (HRESIMS, 1D and 2D NMR), and the absolute configurations were determined by CD spectroscopy. Compounds 2 and 3 showed significant inhibitory activities against HIV-1 with against HIV-1 with IC values of 7.6 and 0.5 μmol/L, respectively. Compounds 2 and 3 also displayed moderate cytotoxicity with CC values of 3.2-303 μmol/L against A549, MDA-MB-231 and PANC-1 cell lines. Moreover, compound 3 can induce the early apoptosis of PANC-1 cancer cells with the apoptosis rate of 28.54%.
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http://dx.doi.org/10.1016/j.fitote.2020.104573DOI Listing
September 2020

Controlling the emission frequency of graphene nanoribbon emitters based on spatially excited topological boundary states.

Phys Chem Chem Phys 2020 Apr 17;22(16):8277-8283. Epub 2020 Mar 17.

Beijing Computational Science Research Center, ZPark II, Beijing 100193, China.

Graphene nanoribbons (GNRs) with atomically precise heterojunction interfaces are exploited as nanoscale light emitting devices with modulable emission frequencies. By connecting GNRs with different widths and lengths, topological boundary states can be formed and manipulated. Using first-principles-based atomistic simulations, we studied the luminescence properties of a STM GNR junction and explored the applications of these topological states as nanoscale light sources. Taking advantage of the ultrahigh resolution of the STM tip, direct injection of high energy carriers at selected boundary states can be achieved. In this way, the emission color can be controlled by precisely changing the tip position. The GNR heterojunction can therefore represent a robust and controllable light-emitting device that takes a step forward towards the fabrication of nanoscale graphene-based optoelectronic devices.
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http://dx.doi.org/10.1039/c9cp06732aDOI Listing
April 2020
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