Publications by authors named "Lei Qiao"

131 Publications

Biogenic selenium nanoparticles by ATCC 393 alleviate the intestinal permeability, mitochondrial dysfunction and mitophagy induced by oxidative stress.

Food Funct 2021 Jun 22. Epub 2021 Jun 22.

The Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an, Shaanxi 710072, China.

Selenium (Se) is an essential trace element. Nano-selenium has attracted great attention due to its various biological properties, especially strong antioxidant activity, high bioavailability, and low toxicity. Our previous studies demonstrated that the selenium nanoparticles (SeNPs) synthesized by Lactobacillus casei ATCC 393 (L. casei ATCC 393) alleviate hydrogen peroxide (H2O2)-induced intestinal epithelial barrier dysfunction via the mitochondrial pathway. However, the mechanism of SeNPs exerting antioxidant activity through the mitochondrial pathway remains unclear. This study was conducted to investigate the role of mitophagy in the protective effects of SeNPs on H2O2-induced porcine intestinal epithelial cells against oxidative damage. The results showed that the SeNPs synthesized by L. casei ATCC 393 had no cytotoxicity on IPEC-J2 cells and effectively antagonized the cytotoxicity of 500 μM H2O2 on IPEC-J2 cells. Moreover, SeNPs attenuated the H2O2-induced intestinal epithelial barrier dysfunction and ROS overproduction, as well as alleviated the adenosine triphosphate (ATP) level and the mitochondrial membrane potential (MMP) decrease. In addition, compared to the oxidative stress model group, pretreatment with biogenic SeNPs significantly up-regulated the expression levels of occludin and claudin-1. Moreover, when compared to the oxidative stress model group, SeNPs inhibited the phosphorylation level of the mammalian target of rapamycin (m-TOR), as well as the expression levels of Unc-51-like kinase 1(ULK1), light chain 3 (LC3)-II/LC3-I, PTEN-induced kinase 1 (PINK1) and Parkin proteins. The fluorescence colocalization images of mitochondria and lysosomes demonstrated that SeNPs significantly reduced the fusion of mitochondria and lysosomes when compared to the oxidative stress model group. These results demonstrate that the SeNPs synthesized by L. casei ATCC 393 can effectively alleviate the H2O2-induced intestinal epithelial barrier dysfunction through regulating mTOR/PINK1-mediated mitophagy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d0fo03141kDOI Listing
June 2021

The in-situ effect of HS on the decomposition of natural rubber and catalyst activity.

Chemosphere 2021 Jun 18;283:131252. Epub 2021 Jun 18.

State Key Laboratory of Coal Combustion, Huazhong University of Science and Technology, 430074, Wuhan, Hubei, China.

In this work, the pyrolysis of natural rubber (NR) under N and HS atmosphere was performed to illustrate the possible effect of HS on NR decomposition with and without catalysts. A molecular dynamics simulation based on reactive force field (ReaxFF) was also conducted to understand the interaction mechanism between HS and intermediates from NR decomposition. Furthermore, the catalytic decomposition of NR under HS atmosphere and the adsorption characteristics of HS by zeolites alone were also carried out to investigate the effect of catalysts on sulfur behavior and the reversed effect of HS on catalyst activity. This work revealed that the introduction of HS can influence the yields of pyrolytic oil and gas, as well as composition of the oil. Combining experimental and simulation studies, HS can interact with intermediates from NR decomposition forming sulfur-containing substances in pyrolytic oil. The HS adsorption experiments by various catalysts revealed that catalysts can chemically adsorb HS. The introduction of Zn can promote the adsorption ability by reacting with sulfur-containing substances to generate ZnS, with the desulfurization effect following the order of 3Zn/ZSM5 > ZSM5 > 3ZnO/ZSM5.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.chemosphere.2021.131252DOI Listing
June 2021

An Ultra-Simple Charge Supplementary Strategy for High Performance Rotary Triboelectric Nanogenerators.

Small 2021 Jul 18;17(29):e2101430. Epub 2021 Jun 18.

CAS Center for Excellence in Nanoscience, Beijing Key Laboratory of Micro-Nano Energy and Sensor, Beijing Institute of Nanoenergy and Nanosystems, Chinese Academy of Sciences, Beijing, 100083, China.

Free-standing rotary triboelectric nanogenerators (rTENG) can accomplish special tasks which require both high voltage and high frequency. However, the reported high performance rTENG all have complex structures for output enhancement. In this work, an ultra-simple strategy to build high performance rTENG is developed. With only one small paper strip added to the conventional structure, the output of the TENG is promoted hugely. The voltage is triplicated to 2.3 kV, and the current and charge are quintupled to 133 µA and 197 nC, respectively. The small paper strip, with the merits of ultra-simplicity, wide availability, easy accessibility and low cost, functions as a super-effective charge supplement. This simple and delicate structure enables ultra-high durability with the 2.3 kV voltage output 100% maintained after 1 000 000 cycles. This charge supplementary strategy is universally effective for many other materials, and decouples the output enhancement from any friction or contact on the metal electrodes, emphasizing a critical working principle for the rTENG. Atmospheric cold plasma is generated using the paper strip rTENG (ps-rTENG), which demonstrates strong ability to do bacteria sterilization. This simple and persistent charge supplementary strategy can be easily adopted by other designs to promote the output even further.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/smll.202101430DOI Listing
July 2021

Preparation, characterization, and evaluation of anti-inflammatory activities of selenium nanoparticles synthesized by GG799.

Food Funct 2021 Jul;12(14):6403-6415

The Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an, Shaanxi 710072, China.

Selenium (Se) is an essential micronutrient that has implications in human diseases, including inflammatory bowel disease (IBD), especially with respect to Se deficiencies. Recently, selenium nanoparticles (SeNPs) have attracted significant attention due to their diversity of biological activities and unique advantages including low toxicity and high biological availability. In this study, an eco-friendly, efficient and low-cost method for synthesis of SeNPs by Kluyveromyces lactis GG799 (K. lactis GG799) was established, and the SeNPs were investigated for their physicochemical properties and anti-inflammatory activities in vivo. K. lactis GG799 was able to successfully transform sodium selenite into bright-red SeNPs with particle sizes of 80 and 150 nm and the nanoparticles accumulated intracellularly. Upon isolation, the SeNPs were found to be mainly capped by proteins and polysaccharides by components analysis. Dietary supplementation with 0.6 mg kg-1 Se (in the form of biogenic SeNPs) effectively attenuated dextran sulphate sodium (DSS)-induced ulcerative colitis (UC) in mice by alleviating oxidative stress and intestinal inflammation. These findings suggested that SeNPs synthesized by K. lactis GG799 may be a promising and safe Se supplement for the prevention and treatment of IBD.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d1fo01019kDOI Listing
July 2021

A Randomized, Double-Blind, and Placebo-Controlled Trial of Chinese Herbal Medicine in the Treatment of Childhood Constipation.

Clin Transl Gastroenterol 2021 May 3;12(5):e00345. Epub 2021 May 3.

Departments of Pediatrics, Shengjing Hospital of China Medical University, Shenyang, P.R. China.

Objectives: Management of constipation is still challenging in childhood. The pharmacological effect of XiaojiDaozhi Decoction, a prescription of Chinese Herbal Medicine (CHM), has been well described for the treatment of food and Qi stagnation which account for childhood constipation. However, the efficacy and safety of XiaojiDaozhi Decoction in childhood constipation remains unclear.

Methods: A randomized, double-blind, and placebo-controlled trial was conducted to evaluate the efficacy and safety of XiaojiDaozhi Decoction in childhood constipation. Two hundred children were recruited and randomly allocated to the CHM or placebo group to receive their respective interventions. The duration of treatment was 8 weeks, with a 12-week follow-up. Main outcome measures were complete spontaneous bowel movements and satisfaction with bowel function. Safety and adverse effects were evaluated by blood laboratory measurements.

Results: At the end of follow-up, the response rates of CHM and placebo were 62% and 31%, respectively (χ2 = 19.315, P < 0.01). At the end of treatment, recurrence was found in 7 cases (10.14%) in CHM and 11 cases (26.19%) in placebo (χ2 = 4.947, P < 0.05). In the main outcome measures, 56 patients (56%) in the CHM group and 25 patients (25%) in the placebo group were satisfied with their bowel movements (χ2 = 19.940, P < 0.05). Increased complete spontaneous bowel movements ≥3 per week from baseline were found in 40 patients (40%) who received CHM and 19 patients (19%) who received placebo (χ2 = 10.602, P < 0.05). No serious adverse effects were found in any of the recruited cases.

Discussion: CHM XiaojiDaozhi Decoction is a safe and effective method for the treatment of childhood constipation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.14309/ctg.0000000000000345DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8096462PMC
May 2021

[BiMo(CO)]: a multiple local σ-aromatic cluster containing a distorted Bi triangular prism.

Chem Commun (Camb) 2021 Apr 16;57(30):3656-3659. Epub 2021 Mar 16.

State Key Laboratory of Elemento-Organic Chemistry, Tianjin Key Lab of Rare Earth Materials and Applications, School of Material Science and Engineering, Nankai University, Tianjin 300350, China.

The first Zintl cluster containing a distorted Bi triangular prism, [BiMo(CO)], has been synthesized and structurally characterized. Quantum chemical calculations indicated that the distorted cage cluster features multiple local σ-aromaticity.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d1cc00734cDOI Listing
April 2021

Diabetes onset at an earlier age and high HbA1c levels as risk factors of diabetic retinopathy.

Int J Ophthalmol 2021 18;14(2):269-276. Epub 2021 Feb 18.

Department of Ophthalmology, the Affiliated Xuzhou Municipal Hospital of Xuzhou Medical University, Xuzhou First People's Hospital, Xuzhou Eye Disease Prevention and Treatment Institute, Xuzhou 221116, Jiangsu Province, China.

Aim: To assess the effect of age at diabetes onset and uncontrollable high HbA1c levels on the development of diabetic retinopathy (DR) among Chinese type 2 diabetes mellitus (DM) patients.

Methods: This was a cross-sectional survey of diabetic patients in Subei district, China. Data covering physical measurements, fasting blood-glucose (FBG), glycosylated hemoglobin (HbA1c), blood lipid, urinary albumin/creatinine ratio (UACR), ocular fundus examination, and diabetes treatment records were collected. An independent sample -test were used to analyze differences. A Logistic regression analysis was applied to study the independent risk factors of DR.

Results: A total of 1282 patients with type 2 DM were enrolled, and 191 cases had DR (14.9%). The age at diabetes onset, education level, alcohol consumption, HbA1c level, UACR level, and hypoglycemic drugs were independent influencing factors for DR. The older the onset of diabetes, the less likely to develop DR (OR: 0.958, 95%CI: 0.942-0.975, =0.000). Patients were then divided in terms of age at diabetes onset as follows: <50y, 50-59y, 60-69y, and ≥70y. Compared with diabetes onset age <50y, 50-59y (OR: 0.463, 95%CI: 0.306-0.699, =0.000), 60-69y (OR: 0.329, 95%CI: 0.203-0.535, =0.000) and ≥70y (OR: 0.232, 95%CI: 0.094-0.577, =0.002) were at a lower risk of DR. The prevalence of DR was highest in patients with diabetes onset age <50y (29.5%, <0.05). The HbA1c level (8.67±1.97)% and proportion of insulin injection (52.5%) in patients with diabetes onset <40y were higher than in patients with older diabetes onset age (<0.05).

Conclusion: Diabetes onset at an earlier age and uncontrollable high HbA1c level could be independent risk factors for DR.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.18240/ijo.2021.02.14DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7840379PMC
February 2021

Personal relative deprivation impairs ability to filter out threat-related distractors from visual working memory.

Int J Psychophysiol 2021 Apr 6;162:86-94. Epub 2021 Feb 6.

Key Laboratory of Cognition and Personality, Ministry of Education, School of Psychology, Southwest University, Chongqing 400715, China. Electronic address:

The literature has indicated that personal relative deprivation (PRD) results in anxiety disorders. Given that some cognitive models propose that attention bias toward a threat causes and maintains anxiety, relatively deprived individuals may have difficulty gating threat from working memory. To test this hypothesis, this study investigated the influence of PRD on the filtering ability of happy, angry, and neutral facial distractors from visual working memory using electroencephalography (EEG). Participants were randomly assigned to a PRD (n = 24) or a non-PRD group (n = 24). Filtering ability was reflected by comparing the contralateral delay activity (CDA) amplitude for one-target, one-target-one-distractor, and two-targets conditions. The CDA was measured as the difference in mean amplitudes between activity in the hemispheres contralateral and ipsilateral to the to-be-remembered information. Results indicated that individuals in the PRD group showed a reduced ability to filter out neutral and angry facial distractors, as reflected by similar CDA amplitudes for one-target-one-distractor and two-targets conditions for both angry and neutral distractors in the PRD group. However, PRD did not impair the ability to filter out happy facial distractors, as reflected by similar CDA amplitudes for one-target-one-distractor and one-target conditions for happy distractors in the PRD group. As neutral faces might then be taken as potentially threatening information by relatively deprived individuals, these results support the hypothesis that relatively deprived individuals might have difficulty filtering out threat-related information.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ijpsycho.2021.02.008DOI Listing
April 2021

Dynamics of a multiplex neural network with delayed couplings.

Appl Math Mech 2021 Feb 2:1-16. Epub 2021 Feb 2.

Department of Engineering Mechanics, College of Mechanics and Materials, Hohai University, Nanjing, 211100 China.

Multiplex networks have drawn much attention since they have been observed in many systems, e.g., brain, transport, and social relationships. In this paper, the nonlinear dynamics of a multiplex network with three neural groups and delayed interactions is studied. The stability and bifurcation of the network equilibrium are discussed, and interesting neural activities of the network are explored. Based on the neuron circuit, transfer function circuit, and time delay circuit, a circuit platform of the network is constructed. It is shown that delayed couplings play crucial roles in the network dynamics, e.g., the enhancement and suppression of the stability, the patterns of the synchronization between networks, and the generation of complicated attractors and multi-stability coexistence.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10483-021-2709-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7852048PMC
February 2021

Unveiling the comparative efficacy and tolerability of comprehensive treatments for migraine: A protocol of systematic review and Bayesian network meta-analysis.

Medicine (Baltimore) 2021 Jan;100(4):e24083

Department of General Surgery, Shengjing Hospital of China Medical University, Shenyang, People's Republic of China.

Background: Migraine is a chronic paroxysmal incapacitating neurological disorder, which endangers the health of human worldwide ranking as the third most prevalent medical condition. There are no comprehensive estimates of treatments for migraine. We will conduct this systematic review and Bayesian network meta-analysis (NMA) to synthesis quantitative and comparative evidence on the efficacy and tolerability of all the known pharmacological and non-pharmacological interventions for migraine.

Method: We will perform the systematic electronic search of the literature utilizing MEDLINE, Embase, Cochrane Central Register of Controlled Trials (CENTRAL), Cumulative Index to Nursing & Allied Health (CINAHL), and PsycINFO. We will only include randomized controlled trials (RCTs) of high quality which appraise the efficacy or safety of any potential pharmacological or non-pharmacological interventions in the treatment of patients with migraine. The traditional pairwise meta-analyses will be performed to anticipate the heterogeneities and publication bias and the NMA will be conducted within a Bayesian hierarchical model framework to obtain estimates for all valuable treatments for migraine. The entire heterogeneity will be quantified by Q statistic and I2 index. Other analyses included sensitivity analyses, meta-regression, and subgroup analyses will also be conducted. The whole process will be conducted using in R-3.6.0 software.

Results: This study will obtain the efficacy and tolerability of all potential treatments for migraine, aiming at providing consolidated evidence to help make the best choice of interventions. The results will be published in a peer-reviewed journal.

Discussion: This Bayesian network meta-analysis may be the first attempt to quantitatively synthesize the efficacy and tolerability of all potential treatments for migraine. And this method can ensure us to fully utilize both the direct and indirect evidence as well as gain the comparative estimates displayed in the derived hierarchies. Besides, we have registered this protocol on the international prospective register of systematic review (PROSPERO) (CRD42020157278).
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/MD.0000000000024083DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7850759PMC
January 2021

Liuzijue qigong versus traditional breathing training for patients with post-stroke dysarthria complicated by abnormal respiratory control: Results of a single-center randomized controlled trial.

Clin Rehabil 2021 Jul 1;35(7):999-1010. Epub 2021 Feb 1.

Department of Rehabilitation, Shanghai Shenyuan Rehabilitation Hospital, Shanghai, China.

Objective: The aim of the study was to investigate whether liuzijue qigong could improve the ability of respiratory control and comprehensive speech in patients with stroke dysarthria.

Design: A randomized controlled trial.

Setting: The research was carried out in the department of rehabilitation.

Participants: Altogether, a total of 98 stroke patients with dysarthria participated in the study.

Interventions: Patients were randomly divided into two groups (the experimental group: basic articulation + liuzijue qigong, 48 patients or the control group: basic articulation + traditional breathing training, 50 patients). All therapies were conducted once a day, five times a week for three weeks.

Main Measures: : Speech breathing level of the modified Frenchay Dysarthria Assessment. : the modified Frenchay Dysarthria Assessment, maximum phonation time, maximal counting ability, /s/, /z/, s/z ratio, and the loudness level. All outcome measures were assessed twice (at baseline and after three weeks).

Results: At three weeks, There were significant difference between the two groups in the change of speech breathing level (81% vs 66%,  = 0.011), the modified Frenchay Dysarthria Assessment (5.54 (4.68-6.40) vs 3.66 (2.92-4.40),  = 0.001), maximum phonation time (5.55 (4.92-6.18) vs 3.01(2.31-3.71),  < 0.01), maximal counting ability (3.08(2.45-3.71) vs 2.10 (1.53-2.67),  = 0.018), and /s/ (3.08 (2.39-3.78) vs 1.87 (1.23-2.51),  = 0.004), while no significant differences were found in the change of /z/ (3.08 (2.31-3.86) vs 2.10 (1.5-2.64),  = 0.08), / ratio (1.26 (0.96-1.55) vs 1.03 (0.97-1.09),  = 0.714), and the change of loudness level (69% vs 60%,  = 0.562).

Conclusions: Liuzijue qigong, combined with basic articulation training, could improve the respiratory control ability, as well as the comprehensive speech ability of stroke patients with dysarthria.

Trial Registration: ChiCTR-INR-16010215.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1177/0269215521992473DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8193589PMC
July 2021

UBE2T promotes proliferation, invasion and glycolysis of breast cancer cells by regualting the PI3K/AKT signaling pathway.

J Recept Signal Transduct Res 2021 Jan 12:1-9. Epub 2021 Jan 12.

Department of Breast, Head and Neck Surgery, Xinjiang Medical University affiliated Tumor Hospital, Urumqi, China.

Purpose: Breast cancer (BCa) is one of the most common gynecological malignancies. Ubiquitin-coupled enzyme E2T (UBE2T) has been demonstrated to play crucial roles in various tumors.

Methods: UBE2T levels were detected using quantitative real time PCR and western blot. CCK-8 and colony formation assays were used to evaluate cell proliferation. A xenograft model was used to evaluate the effects of UBE2T on tumor growth in mice, and immunohistochemistry (IHC) assay was performed to detect the expression of UBE2T and Ki-67. Transwell assay was performed to determine cell migration and invasion. The ATP level, glucose consumption and lactate production were measured using the corresponding commercial kits. Western blot assay was used to detect the levels of epithelial-mesenchymal transformation (EMT), glycolytic and the PI3K/AKT pathway related proteins regulated by UBE2T.

Results: Upregulation of UBE2T expression in human BCa tissues was found in human clinical BCa tissues and The Cancer Genome Atlas (TCGA) dataset. The expression of UBE2T was confirmed to be up-regulated in BCa cells compared to normal breast epithelial cell line (MCF-10A). Overexpression of UBE2T promoted proliferation, migration, invasion and glycolysis in BCa cells, while UBE2T knockdown showed the opposite results. Moreover, UBE2T knockdown suppressed tumor growth in mice. Further mechanism analysis shows that UBE2T participated in the regulation of BCa progression through affecting the PI3K/AKT signaling pathway.

Conclusion: UBE2T promoted proliferation, invasion and glycolysis through modulating PI3K/AKT signaling pathway in BCa, implying that UBE2T may provide a promising therapeutic target for the therapy of BCa.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/10799893.2020.1870495DOI Listing
January 2021

Controlling single-photon scattering in a rectangular waveguide by a V-type three-level emitter.

Authors:
Ya-Ju Song Lei Qiao

Opt Express 2020 Dec;28(25):37639-37653

The single-photon scattering by a V-type three-level emitter in a rectangular waveguide is studied. Here the frequency value of input photons can be large beyond the single-transverse-mode region. By using Green's function formalism, the necessary and sufficient conditions of complete transmission as well as complete reflection are derived analytically. In the region of single transverse mode, the physical mechanisms of complete transmission and complete reflection are electromagnetically induced transparency (EIT) and Fano resonance, respectively. In the region of multiple transverse modes, which are induced by the finite cross section, the quantum interference between multiple scattering pathways with different transverse modes can be used to manipulate the single-photon transport. We find that the emitter becomes transparent when the superposition of waveguide modes has zero amplitude at the position of emitter. And the perfect reflection is absent even under Fano resonance unless the input-state is in a coherent superposition state. These results may promote the development of single-photon devices with wide applicable frequency region.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1364/OE.404467DOI Listing
December 2020

Familial Idiopathic Intracranial Hypertension in Two Non-obese Chinese Sisters.

Front Neurol 2020 26;11:569432. Epub 2020 Nov 26.

Department of Neurology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Familial idiopathic intracranial hypertension (FIIH) is a rare condition, the etiology of which is unclear. To describe two non-obese Chinese sisters who met the criteria of FIIH and to analyze the clinical features and prognosis of FIIH. The clinical course, treatment, and prognosis of these two patients were analyzed retrospectively. Meanwhile, all the literature of familial IIH (FIIH) was reviewed. These two sisters presented with headaches and visual impairment in their mid-thirties. Magnetic resonance imaging (MRI) of the brain was unremarkable except for partial empty sella. No comorbidities or defined causes were detected. Headaches were partially relieved by dehydrated medicine, whereas the visual impairment persisted. In cases where patients present with headaches, empty sella are found on an MRI, and there is visual impairment with or without papilla edema, intracranial hypertension should be excluded. Furthermore, we should pay more attention to the relatives of those patients with increased intracranial hypertension.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fneur.2020.569432DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7726185PMC
November 2020

Spontaneous brain state oscillation is associated with self-reported anxiety in a non-clinical sample.

Sci Rep 2020 11 12;10(1):19754. Epub 2020 Nov 12.

Key Laboratory of Cognition and Personality, Ministry of Education, Department of Psychology, Southwest University, Chongqing, 400715, China.

The anti-correlation relationship between the default-mode network (DMN) and task-positive network (TPN) may provide valuable information on cognitive functions and mental disorders. Moreover, maintaining a specific brain state and efficaciously switching between different states are considered important for self-regulation and adaptation to changing environments. However, it is currently unclear whether competitions between the DMN and TPN are associated with negative affect (here, anxiety and depression) in non-clinical samples. We measured the average dwell time of DMN dominance over the TPN (i.e., the average state duration before transition to another state, indicating persistent DMN dominance) with a sample of 302 non-clinical young adults. Subsequently, we explored individual differences in this persistent DMN dominance by examining its correlations with subjective depression and anxiety feelings. Moreover, we linked state transition between DMN/TPN dominance with right fronto-insular cortex (RFIC) blood oxygen-level dependent signal variability. We found that the average dwell time of DMN dominance was positively associated with self-reported anxiety. Furthermore, state transition between DMN or TPN dominance was positively linked to RFIC activity. These findings highlight the importance of investigating the complex and dynamic reciprocal inhibition patterns of the DMN and TPN and the important role of the RFIC in the association between these networks.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-020-76211-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7661527PMC
November 2020

A sandwich-type cluster containing [email protected] planar fragment flanked by aromatic nonagermanide caps.

Nat Commun 2020 10 20;11(1):5286. Epub 2020 Oct 20.

Tianjin Key Lab for Rare Earth Materials and Applications, State Key Laboratory of Elemento-Organic Chemistry, School of Materials Science and Engineering, Nankai University, 300350, Tianjin, China.

Sandwich-type clusters with the planar fragment containing a heterometallic sheet have remained elusive. In this work, we introduce the [K(2,2,2-crypt)]{(Ge)[η-Ge(PdPPh)]} complex that contains a heterometallic sandwich fragment. The title compound is structurally characterized by means of single-crystal X-ray diffraction, which reveals the presence of an unusual heteroatomic metal planar fragment [email protected] The planar fragment contains a rare formal zerovalent germanium core and a peculiar bonding mode of [email protected](PdPPh) trigonal planar structure, whereas the nonagermanide fragments act as capping ligands. The chemical bonding pattern of the planar fragment consists of three 2c-2e Pd-Ge σ-bonds attaching Pd atoms to the core Ge atom, while the binding between the planar fragment and the aromatic Ge ligands is provided by six 2c-2e Pd-Ge σ-bonds and two delocalized 4c-2e σ-bonds. The synthesized cluster represents a rare example of a sandwich compound with the heteroatomic metal planar fragment and inorganic aromatic capping ligands.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41467-020-19079-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7575547PMC
October 2020

IL-6-induced acetylation of E2F1 aggravates oxidative damage of retinal pigment epithelial cell line.

Exp Eye Res 2020 11 8;200:108219. Epub 2020 Sep 8.

Xuzhou Key Laboratory of Ophthalmology, The Affiliated Xuzhou Municipal Hospital of Xuzhou Medical University, Xuzhou First People's Hospital, Eye Institute of Xuzhou, Xuzhou, 221100, China; Department of Ophthalmology, The Affiliated Xuzhou Municipal Hospital of Xuzhou Medical University, Xuzhou First People's Hospital, Eye Institute of Xuzhou, Xuzhou, 221100, China. Electronic address:

Oxidative damage in retinal pigment epithelial cells (RPE) is considered to be a crucial pathogenesis of age-related macular degeneration (AMD). Although dysregulation of the DNA repair system has been found in RPE cells of AMD patients, the detailed molecular mechanisms of this dysregulation and their relationship with the intraocular microenvironment of AMD patients remain unclear. Here, we established an RPE model of HO-induced oxidative stress and found that Sirtuin 1 (Sirt1)-mediated deacetylation of E2F transcription factor 1 (E2F1) was required for oxidation resistance in RPE cells. Moreover, E2F1 induced the expression of the chromatin-binding protein, high mobility group AT-Hook 1 (HMGA1), which promoted the transcription of glucose 6-phosphate dehydrogenase (G6PD), the rate-limiting enzyme of the pentose phosphate pathway, to increase NADPH level for antioxidant defense. Interrupting the E2F1/HMGA1/G6PD regulatory axis increased reactive oxygen species (ROS) levels, DNA damage, and apoptosis in RPE cells under oxidative stress. Notably, interleukin 6 (IL-6), an inflammatory cytokine that is known to be upregulated in the intraocular fluid of AMD patients, induced phosphorylation (S47) of Sirt1 by activating PI3K/AKT/mTOR signaling, thereby inhibiting Sirt1 activity and increasing the acetylation of E2F1. Specific inhibitors of PI3K/AKT/mTOR signaling decreased DNA damage and ROS while increasing NADPH in RPE cells. Collectively, our findings demonstrate that IL-6-induced acetylation of E2F1 impairs the antioxidant capacity of RPE cells by disturbing the pentose phosphate pathway, which elucidates a relationship between the intraocular microenvironment and RPE oxidative damage in AMD and provides a possible therapeutic target for AMD.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.exer.2020.108219DOI Listing
November 2020

Comparative Effect of Liuzijue Qigong and Conventional Respiratory Training on Trunk Control Ability and Respiratory Muscle Function in Patients at an Early Recovery Stage From Stroke: A Randomized Controlled Trial.

Arch Phys Med Rehabil 2021 03 12;102(3):423-430. Epub 2020 Aug 12.

Department of Rehabilitation, Shanghai Xuhui Central Hospital, Shanghai, China.

Objectives: To compare the effects of Liuzijue Qigong and conventional respiratory training on trunk control ability and respiratory muscle functions in patients at an early recovery stage from stroke.

Design: A single-blind, randomized controlled trial.

Setting: A hospital.

Participants: Patients (N=60) within 2 months poststroke.

Interventions: The experimental group (n=30) received conventional rehabilitation training combined with Liuzijue exercise, and the control group (n=30) received conventional rehabilitation training combined with conventional respiration training. The training in the 2 groups was conducted 5 times per week for 3 weeks.

Main Outcome Measures: Trunk Impairment Scale (TIS), maximum inspiratory pressure (MIP), maximum expiratory pressure (MEP), forced expiratory volume in the first second (FEV1), forced vital capacity (FVC), peak expiratory flow (PEF), maximum expiratory mid-flow (MMEF), diaphragmatic movement, the change of intra-abdominal pressure (IAP), Berg Balance Scale (BBS), and Modified Barthel Index (MBI). All outcome measures were assessed twice (at baseline and 3 weeks).

Results: Both groups significantly improved in TIS, MIP, FVC, PEF, and the change of IAP, BBS, and MBI when pre- and postassessments (P<.05) were compared. Compared with the control group, there was a significant difference in the experimental group in the static sitting balance subscale (P=.014), dynamic balance subscale (P=.001), coordination subscale (P<.001), TIS total scores (P<.001; effect size [ES]=0.9), MIP (P=.012; 95% confidence interval [CI], 2.23-17.69; ES=0.67), MEP (P=.015; 95% CI, 1.85-16.57; ES=0.65), change of IAP (P=.001), and MBI (P=.016; 95% CI, 1.51-14.16; ES=0.64). No significant differences were found between the 2 groups in FEV1 (P=.24), FVC (P=.43), PEF (P=.202), MMEF (P=.277), the diaphragmatic movement of quiet breathing (P=.146), deep breathing (P=.102), and BBS (P=.124).

Conclusions: Liuzijue exercise showed more changes than conventional respiratory training in improving trunk control ability, respiratory muscle functions, and activities of daily living ability in patients at an early recovery stage from stroke.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.apmr.2020.07.007DOI Listing
March 2021

Effect of strengthening detail management on nursing quality and nursing satisfaction in disinfection supply room.

Panminerva Med 2020 Jul 22. Epub 2020 Jul 22.

Central Sterile Supply Department, Yantai Yuhuangding Hospital Affiliated to Qingdao University, Yantai, China -

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.23736/S0031-0808.20.03952-XDOI Listing
July 2020

[[email protected]] (E = Sn, Pb): Fused Derivatives of Endohedral Stannaspherene and Plumbaspherene.

J Am Chem Soc 2020 Aug 27;142(31):13288-13293. Epub 2020 Jul 27.

Tianjin Key Lab for Rare Earth Materials and Applications, State Key Laboratory of Elemento-Organic Chemistry, School of Materials Science and Engineering, Nankai University, Tianjin 300350, China.

We report the synthesis of two new cluster anions, [[email protected]] (E = Sn, Pb), in which a Cu subunit is incorporated into a continuous E tetrel cage. Both anions are characterized by X-ray crystallography and mass spectrometry, complemented by quantum-chemical calculations that highlight the relationships to known Zintl clusters, including the stannaspherenes and plumbaspherenes [[email protected]] and [[email protected]], the Matryoshka bronze [[email protected]@Sn], and also [[email protected]'] (E' = Ge, Sn).
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/jacs.0c04815DOI Listing
August 2020

Flexible adjustment of the effective connectivity between the fronto-parietal and visual regions supports cognitive flexibility.

Neuroimage 2020 10 11;220:117158. Epub 2020 Jul 11.

Key Laboratory of Cognition and Personality, Ministry of Education, Faculty of Psychology, Southwest University, Chongqing, China. Electronic address:

Evidence indicates the significance of the fronto-parietal regions and inertia sensory processing from previous trials in cognitive flexibility. However, how flexible cognitive performance is achieved by causal interactions between cortical regions, particularly those between the fronto-parietal and stimulus processing regions, remains unknown. In the current study, the effective connectivity between the fronto-parietal and visual regions was examined in the context of a cued task-switching paradigm. We found that the fronto-parietal and visual cortex were differently activated during task transition (task repeat and task switch). Importantly, dynamic causal modeling (DCM) analysis revealed that task transition could modulate the effective connectivity between the fronto-parietal and visual cortex: task repeat decreased, while task switch enhanced, the coupling between the posterior parietal cortex (PPC) and the visual cortex. Furthermore, Granger causality analysis (GCA) showed that the dominant direction of influence was from the fronto-parietal regions to the visual cortex. Finally, individual differences in the top-down influence from the PPC to the visual cortex and the corresponding neural adjustment (task switch‒task repeat) was negatively associated with the behavioral switch cost. Our findings suggest that the interaction between the fronto-parietal and stimulus processing regions, particularly the top-down influence from the PPC to the visual cortex, is of particular importance in flexible cognitive performance.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.neuroimage.2020.117158DOI Listing
October 2020

A family of lead clusters with precious metal cores.

Nat Commun 2020 07 10;11(1):3477. Epub 2020 Jul 10.

Tianjin Key Lab for Rare Earth Materials and Applications, State Key Laboratory of Elemento-Organic Chemistry, School of Materials Science and Engineering, Nankai University, Tianjin, 300350, China.

Gold nanoparticles have been used for centuries, both for decoration and in medical applications. More recently, many of the major advances in cluster chemistry have involved well-defined clusters containing tens or hundreds of atoms, either with or without a ligand shell. In this paper we report the synthesis of two gold/lead clusters, [AuPb] and [AuPb], both of which contain nido [[email protected]] icosahedra surrounding a core of Au atoms. Analogues of these large clusters are not found in the corresponding Ag chemistry: instead, the Ag-centered nido icosahedron, [[email protected]], is the only isolated product. The structural chemistry, along with the mass spectrometry which shows the existence of [AuPb] but not [AgPb], leads us to propose that the former species is the key intermediate in the growth of the larger clusters. Density functional theory indicates that secondary π-type interactions between the [[email protected]] ligands and the gold core play a significant part in stabilizing the larger clusters.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41467-020-17187-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7351731PMC
July 2020

LncWNT3-IT affects the proliferation of Sertoli cells by regulating the expression of the WNT3 gene in goat testis.

Reprod Domest Anim 2020 Sep 11;55(9):1061-1071. Epub 2020 Aug 11.

College of Animal Science and Technology, Southwest University, Chongqing, China.

The proliferation and differentiation ability of testicular Sertoli cells directly affects spermatogenesis and male reproductive development. WNT proteins are involved in the regulation of cell proliferation, differentiation and spermatogenesis. Therefore, to study whether lncRNAs, which regulate the expression of WNT proteins during cell proliferation and differentiation, are worthwhile. In this study, testicular tissue from the Dazu black goat (Capra, goat, Chongqing, China) at neonatal time (less than 7 days old), early puberty time (45 days old) and sexual maturity time (90 days old) at three ages was subjected to high-throughput sequencing to predict testicular growth and development associated with WNT lncRNA. The final screening of lncWNT3-IT may be targeted to regulate the expression of WNT3. At the same time, the expression of WNT3 was verified by lncWNT3-IT by paraffin sectioning, fluorescence in situ hybridization, interference, overexpression, cytotoxicity assay, Western blotting and qPCR. The following results were obtained: lncWNT3-IT was expressed in the testicular Sertoli cells and played a role in the Sertoli cell cytoplasm. Fluorescence in situ hybridization localization analysis showed that lncWNT3-IT positively regulated the expression of WNT3, and through cell viability and cell proliferation experiments, it was found that the expression of lncWNT3-IT assisted in Sertoli cell proliferation. In summary, lncWNT3-IT can influence the proliferation of Sertoli cells by positively regulating the expression of WNT3.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/rda.13738DOI Listing
September 2020

All-metal σ-antiaromaticity in dimeric cluster anion {[CuGeMes]}.

Chem Commun (Camb) 2020 Jun;56(48):6583-6586

School of Materials Science and Engineering, State Key Laboratory of Element-Organic Chemistry, Tianjin Key Lab for Rare Earth Materials and Applications, Nankai University, Tianjin 300350, China.

In this work, we report a dimeric cluster anion, {[CuGe9Mes]2}4-, which was isolated as the [K(2,2,2-crypt)]+ salt and characterized by using single-crystal X-ray diffraction and ESI mass spectroscopy. The title cluster represents the first locally σ-antiaromatic compound in the solid state, as well as the first heteroatomic antiaromatic compound.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d0cc02525aDOI Listing
June 2020

Phase-Controlled Synthesis of Monolayer W Re S Alloy with Improved Photoresponse Performance.

Small 2020 May 22;16(20):e2000852. Epub 2020 Apr 22.

School of Materials Science and Engineering, Shanghai University, Shanghai, 200444, China.

Tuning bandgap and phases in the ternary 2D transition metal dichalcogenides (TMDs) alloys has opened up unexpected opportunities to engineer optoelectronic properties and explore potential applications. In this work, a salt-assisted chemical deposition vapor (CVD) growth strategy is reported for the creation of high-quality monolayer W Re S alloys to fulfill a readily phase control from 1H to DT by changing the ratio of Re and W precursors. The structures and chemical compositions of doping alloys are confirmed by combining atomic resolution scanning transmission electron microscopy-annular dark field imaging with energy dispersive X-ray spectroscopy (EDS) and X-ray photoelectron spectroscopy, matching well with the calculated results. The field-effect transistors (FETs) devices fabricated based on 1H-W Re S monolayer exhibit a n-type semiconducting behavior with the mobility of 0.4 cm V s . More importantly, the FETs show high-performance responsivity with a value of 17 µA W in air, which is superior to that of monolayer CVD-grown WS . This work paves the way toward synthesizing monolayer ternary alloys with controlled phases for potential optoelectronic applications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/smll.202000852DOI Listing
May 2020

Non-motor Symptoms in Chinese Patients With Isolated Generalized Dystonia: A Case-Control Study.

Front Neurol 2020 8;11:209. Epub 2020 Apr 8.

Department of Neurology, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, China.

Previous studies have indicated that non-motor symptoms are primary problems in focal dystonia, but limited data are available about non-motor problems and their correlation with motor severity in generalized dystonia (GD). In the present study, we performed a case-control study and enrolled isolated inherited or idiopathic GD patients and age- and sex-matched healthy controls (HC). Clinical characteristics, motor symptoms, non-motor problems, including psychiatric co-morbidity, sleep problems, fatigue, and quality of life (QoL) were assessed in both groups using various rating scales and assessments. Thirty-three patients with GD and 33 controls were enrolled. Significant higher scores on depression and anxiety ( < 0.001) were shown in GD compared with HC, whereas the frequency of obsessive-compulsive disorders approached that of HC ( = 0.238). Patients with GD also had significantly higher Pittsburg Sleep Quality Index (PSQI) and fatigue scores than HC, whereas no difference was observed in excessive daytime somnolence. In GD, QoL was more impaired, with statistically lower scores in both physical and mental components. Psychiatric rating scales did not correlate to motor severity or disease duration but might influence quality of sleep. Subgroup analysis suggests non-motor manifestations differ with different etiologies in GD. This study suggests that non-motor symptoms in GD, such as psychiatric problems, are likely to be primary determinants not correlated to motor severity, which may also affect quality of sleep and fatigue.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fneur.2020.00209DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7156613PMC
April 2020

Deficient Chaperone-Mediated Autophagy Promotes Lipid Accumulation in Macrophage.

J Cardiovasc Transl Res 2020 Apr 13. Epub 2020 Apr 13.

The Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education, Chinese National Health Commission and Chinese Academy of Medical Sciences, The State and Shandong Province Joint Key Laboratory of Translational Cardiovascular Medicine, Department of Cardiology, Qilu Hospital of Shandong University, 107 Wenhuaxi Road, 250012, Jinan, China.

Chaperone-mediated autophagy (CMA) serves as a critical upstream regulator of lipophagy and lipid metabolism in hepatocyte. However, the role of CMA in lipid metabolism of macrophage, the typical component of atherosclerotic plaque, remains unclear. In our study, LAMP-2A (L2A, a CMA marker) was reduced in macrophages exposed to high dose of oleate, and lipophagy was impaired in advanced atherosclerosis in ApoE (-/-) mice. Primary peritoneal macrophages isolated from macrophage-specific L2A-deficient mice exhibited pronounced intracellular lipid accumulation. Lipid regulatory enzymes, including long-chain-fatty-acid-CoA ligase 1 (ACSL1) and lysosomal acid lipase (LAL), were increased and reduced in L2A-KO macrophage, respectively. Other lipid-related proteins, such as SR-A, SR-B (CD36), ABCA1, or PLIN2, were not associated with increased lipid content in L2A-KO macrophage. In conclusion, deficient CMA promotes lipid accumulation in macrophage probably by regulating enzymes involved in lipid metabolism. CMA may represent a novel therapeutic target to alleviate atherosclerosis by promoting lipid metabolism. Graphical abstract.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12265-020-09986-3DOI Listing
April 2020

Biogenic selenium nanoparticles synthesized by Lactobacillus casei ATCC 393 alleviate diquat-induced intestinal barrier dysfunction in C57BL/6 mice through their antioxidant activity.

Food Funct 2020 Apr;11(4):3020-3031

The Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an, Shaanxi 710072, China.

Selenium (Se) as an essential micronutrient plays a crucial role in human health. Biogenic selenium nanoparticles (SeNPs) possess attractive biological properties, biocompatibility, stability and low-toxicity. This study was aimed to investigate the protective effect of biogenic SeNPs of size 50-80 nm synthesized by Lactobacillus casei ATCC 393 (L. casei ATCC 393) on diquat-induced intestinal barrier dysfunction in C57BL/6 mice and the intrinsic mechanisms. Our results showed that oral administration of SeNPs significantly inhibited the increase of the serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), diamine oxidase (DAO) and d-lactic acid (d-LA) levels induced by diquat, and increased the total superoxide dismutase (T-SOD), thioredoxin reductase (TrxR) and glutathione peroxidase (GSH-Px) activities in serum and jejunum. Moreover, SeNPs increased the number of goblet cells, decreased the production of reactive oxygen species (ROS), maintained the mitochondrial functions, and improved the expression levels of occludin and claudin-1 in jejunum compared to the diquat-induced oxidative stress model group. In addition, SeNPs activated the nuclear factor (erythroid-derived-2)-like 2 (Nrf2), and improved the protein levels of heme oxygenase (HO)-1 and NADPH dehydrogenase (NQO)-1 compared to other treatment groups. These results suggested that biogenic SeNPs synthesized by L. casei ATCC 393 can protect the intestinal barrier function against oxidative damage via Nrf2-mediated signaling pathway.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d0fo00132eDOI Listing
April 2020

Characterization of thermostable Newcastle disease virus recombinants expressing the hemagglutinin of H5N1 avian influenza virus as bivalent vaccine candidates.

Vaccine 2020 02 11;38(7):1690-1699. Epub 2020 Jan 11.

State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China. Electronic address:

Newcastle disease virus (NDV) has been used as a vector in the development of vaccines and gene delivery. In the present study, we generated the thermostable recombinant NDV (rNDV) expressing the different forms of hemagglutinin (HA) of highly pathogenic avian influenza virus (HPAIV) H5N1 based on the full-length cDNA clone of thermostable TS09-C strain. The recombinant thermostable Newcastle disease viruses, rTS-HA, rTS-HA1 and rTS-tPAs/HA1, expressed the HA, HA1 or modified HA1 protein with the tissue plasminogen activator signal sequence (tPAs), respectively. The rNDVs displayed similar thermostability, growth kinetics and pathogenicity compared with the parental TS09-C virus. The tPAs facilitated the expression and secretion of HA1 protein in cells infected with rNDV. Animal studies demonstrated that immunization with rNDVs elicited effective H5N1- and NDV-specific antibody responses and conferred immune protection against lethal H5N1 and NDV challenges in chickens and mice. Importantly, vaccination of rTS-tPAs/HA1 resulted in enhanced protective immunity in chickens and mice. Our study thus provides a novel thermostable NDV-vectored vaccine candidate expressing a soluble form of a heterologous viral protein, which will greatly aid the poultry industry in developing countries.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.vaccine.2019.12.054DOI Listing
February 2020

Lactobacillus casei ATCC 393 alleviates Enterotoxigenic Escherichia coli K88-induced intestinal barrier dysfunction via TLRs/mast cells pathway.

Life Sci 2020 Mar 8;244:117281. Epub 2020 Jan 8.

The Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an, Shaanxi 710072, China.

Aims: Mast cells play a crucial role in gastrointestinal physiology and pathophysiology. This study was conducted to investigate the role of mast cells (MCs) in the protective effect of Lactobacillus casei ATCC 393 (L. casei ATCC 393) on intestinal barrier function.

Main Methods: The regulatory effect of L. casei ATCC 393 on intestinal barrier dysfunction and MCs activation induced by Enterotoxigenic Escherichia coli K88 (ETEC K88) were evaluated by porcine mucosal mast cells (PMMCs)-porcine jejunal epithelial cells (IPEC-J2)-L. casei ATCC 393 co-culture experiments in vitro and MCs stabilizer drug experiment in vivo.

Key Findings: Results showed that L. casei ATCC 393 pretreatment effectively alleviated the reduction of cell viability and increase of permeability in ETEC K88-infected IPEC-J2 cells. L. casei ATCC 393 pretreatment inhibited the increase of proinflammatory cytokines and some other MCs mediators, and decrease of anti-inflammatory cytokines in ETEC K88-infected PMMCs. Cromolyn sodium or L. casei ATCC 393 prevented ETEC K88-induced increase of intestinal epithelial cell permeability in IPEC-J2 cells when co-cultivation with PMMCs. Furthermore, cromolyn sodium or L. casei ATCC 393 pretreatment attenuated ETEC K88-induced increase of MCs mediators, mast cell proteases (MCPs) and carboxypeptidase A3 (CPA3) mRNA levels, and down-regulation of tight junction proteins, Toll-like receptor 2 and 4 (TLR2 and TLR4) expression levels in mice challenged by ETEC K88.

Significance: These results indicated that intestinal barrier dysfunction caused by ETEC K88 was mediated by intestinal mast cell activation which can be prevented by L. casei ATCC 393 via TLRs signaling pathway.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.lfs.2020.117281DOI Listing
March 2020