Publications by authors named "Lei Pan"

412 Publications

Membranous Nephropathy Complicated with Disseminated Infection: A Case Report and Literature Review.

Infect Drug Resist 2021 8;14:4157-4166. Epub 2021 Oct 8.

Department of Respiratory and Critical Care Medicine, The First Hospital of China Medical University, Shenyang, People's Republic of China.

Disseminated infection caused by with primary nephrotic syndrome is exceedingly rare. A 66-year-old female visited the outpatient department due to fever and fatigue who had been diagnosed as membranous nephropathy and with a long-term prednisone and immunosuppressive therapy. After lung biopsy for many times, culture from space-occupying lesion of the right lung and species identification by mass spectrometry-based methods (MALDI-TOF) revealed . By imaging examination, space-occupying lesions from the lungs, brain, abdominal cavity and kidney were found. After 2 weeks of meropenem intravenous and up to 6 months of trimethoprim-sulfamethoxazole (TMP-SMX) therapy, our patient has remained relapse-free at that time of writing. Disseminated infection caused by is usually subacute with complex clinical manifestations. In addition, it can be easily confused with diseases such as tumor and mycobacterial infection, and lead to fatal consequences. Therefore, we hope that we can remind clinicians considering by discussing common features of disseminated infection.
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http://dx.doi.org/10.2147/IDR.S331737DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8517639PMC
October 2021

Extracorporeal Membrane Oxygenation for Acute Toxic Inhalations: Case Reports and Literature Review.

Front Med (Lausanne) 2021 29;8:745555. Epub 2021 Sep 29.

Department of Emergency and Critical Care Medicine, Jinshan Hospital, Fudan University, Shanghai, China.

Previous studies have shown that poisoning is a major threat to human health. Inhalation of acute toxic gas has been linked to serious health consequences. Among the antidotes for poisoning currently used, supportive care is the most common intervention in clinical practice. Severe acute respiratory distress syndrome (ARDS) and/or refractory cardiogenic shock or cardiac arrest caused by toxins are associated with high mortality and are difficult to treat. Extracorporeal membrane oxygenation (ECMO) is an aggressive supportive measure used to manage severely poisoned patients. This study presents two cases of acute toxic gases inhalation, severe ARDS and circulatory instability induced by bromine inhalation, and ARDS induced by nitric acid inhalation which were successfully treated with ECMO. The ECMO techniques used in the animal models and in human cases to treat severe poisoning are described as well as the indications, contraindications, complications, and weaning of ECMO.
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http://dx.doi.org/10.3389/fmed.2021.745555DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8511675PMC
September 2021

Chylothorax caused by left atrial myxoma: A rare case report.

J Card Surg 2021 Oct 13. Epub 2021 Oct 13.

Department of Cardiothoracic Surgery, Xiaogan Hospital Affiliated to Wuhan University of Science and Technology, Xiaogan Central Hospital, Xiaogan, Hubei, PR China.

Background: Myxoma is the most common tumor of the heart that can cause embolism, obstruction, and cardiac failure, but rarely causes chylothorax. We herein report a case of chylothorax caused by left atrial myxoma, which responded to diuretic therapy and was subsequently cured by resecting cardiac myxoma.

Case Presentation: This is a case of a 63-year-old male with symptoms of cardiac insufficiency who was diagnosed with a left atrial myxoma. The patient also had a massive pleural effusion on the right side, which was diagnosed as chylothorax by Sudan III staining and the content of triglyceride. The pleural effusion disappeared and the symptoms of heart failure were relieved after draining the effusion with a fine thoracic drainage tube, but pleural effusion and cardiac insufficiency symptoms relapsed soon. Although diuretic treatment can improve the symptoms and reduced the amount of chylous fluid preoperatively, the chylous pleural effusion was eventually cured by surgical removal of the tumor, and no recurrence of the tumor and chylothorax was found in the 6-month follow-up.

Conclusions: Chylothorax is rare comorbidity of cardiac myxoma. Large myxoma can cause congestive heart failure and lead to disfunction of chylous reflux, resulting in chylothorax, which can be cured by resection of the primary tumor.
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http://dx.doi.org/10.1111/jocs.16074DOI Listing
October 2021

Spectral fitting method for obtaining the refractive index and thickness of chalcogenide films.

Opt Express 2021 Aug;29(18):29329-29340

Spectral fitting method (SFM) was proposed to obtain the refractive index (RI) and thickness of chalcogenide films based on transmission spectra. It extended the Swanepoel method to the films on the order of hundreds of nanometers in thickness. The RI and thickness of the films can be obtained quickly and accurately by using the SFM based on the transmission spectrum with only one peak and valley. The method's reliability theoretically was evaluated by simulation analysis. The results showed that the accuracy of the RI and thickness was better than 0.2% by using the SFM regardless of thin or thick film. Finally, the RI and thickness of the new ultralow loss reversible phase-change material SbSe films were obtained experimentally by the SFM. This work should provide a useful guideline for obtaining the RI and thickness of the transparent optical films.
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http://dx.doi.org/10.1364/OE.438391DOI Listing
August 2021

Portable Smartphone Platform Based on a Single Dual-Emissive Ratiometric Fluorescent Probe for Visual Detection of Isopropanol in Exhaled Breath.

Anal Chem 2021 Oct 5. Epub 2021 Oct 5.

Institute of Solid State Physics, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui 230031, China.

The components in the exhaled breath have been confirmed to be related to certain diseases, especially studies have shown that isopropanol (IPA) might be closely associated with illnesses such as lung cancer, and are considered as a biomarker. Herein, we designed a portable smartphone platform based on a chemically synthesized ratiometric fluorescent probe for real-time/on-site, sensitive, and quantitative visual detection of IPA in exhaled breath. The fluorescent probe was fabricated by a nicotinamide adenine dinucleotide (NAD) functional modified onto fluorescent internal standard red carbon dots (RCDs). Whereas, IPA can convert NAD into reduced nicotinamide adenine dinucleotide (NADH) through an enzymatic reaction of secondary alcohol dehydrogenase (S-ADH). The electron transfer from IPA to NAD emitted a blue emission of NADH, which displayed consecutive color changes from red to light blue. Under optimum conditions, the fluorescent probe shows sensitive responses to IPA with a detection limit as low as 4.45 nM. Moreover, combined with the smartphone color recognizer application (APP), the ratio of fluorescence intensity response was recorded on a blue channel (B)/red channel (R), which has been employed for the visual quantitative determination of IPA with a detection limit of 8.34 nM and a recovery rate of 90.65-110.09% (RSD ≤ 4.83). The method reported here provides a convenient pathway for real-time/on-site and visual detection of IPA in exhaled air and is expected to extend the application of investigation of potential volatile biomarkers for preliminary monitoring and clinical diagnosis.
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http://dx.doi.org/10.1021/acs.analchem.1c03280DOI Listing
October 2021

Prediction of response after cardiac resynchronization therapy with machine learning.

Int J Cardiol 2021 Sep 28. Epub 2021 Sep 28.

Department of Cardiology, Zhongshan Hospital of Fudan University, Shanghai Institute of Cardiovascular Diseases, National Clinical Research Center for Interventional Medicine, Shanghai, China.

Aims: Nearly one third of patients receiving cardiac resynchronization therapy (CRT) suffer non-response. We intend to develop predictive models using machine learning (ML) approaches and easily attainable features before CRT implantation.

Methods And Results: The baseline characteristics of 752 CRT recipients from two hospitals were retrospectively collected. Nine ML predictive models were established, including logistic regression (LR), elastic network (EN), lasso regression (Lasso), ridge regression (Ridge), neural network (NN), support vector machine (SVM), random forest (RF), XGBoost and k-nearest neighbour (k-NN). Sensitivity, specificity, precision, accuracy, F1, log-loss, area under the receiver operating characteristic (AU-ROC), and average precision (AP) of each model were evaluated. AU-ROC was compared between models and the latest guidelines. Six models had an AU-ROC value above 0.75. The LR, EN and Ridge models showed the highest overall predictive power compared with other models with AU-ROC at 0.77. The XGBoost model reached the highest sensitivity at 0.72, while the highest specificity was achieved by Ridge model at 0.92. All ML models achieved higher AU-ROCs that those derived from the latest guidelines (all P < 0.05). The effect size analysis identified left bundle branch block, left ventricular end-systolic diameter, and history of percutaneous coronary intervention as the most crucial predictors of CRT response. An online tool to facilitate the prediction of CRT response is freely available at http://www.crt-response.com/.

Conclusions: ML algorithms produced efficient predictive models for evaluation of CRT response with features before implantation. Tools developed accordingly could improve the selection of CRT candidates and reduce the incidence of non-response.
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http://dx.doi.org/10.1016/j.ijcard.2021.09.049DOI Listing
September 2021

Downregulation of Perilipin1 by the Immune Deficiency Pathway Leads to Lipid Droplet Reconfiguration and Adaptation to Bacterial Infection in .

J Immunol 2021 Nov 29;207(9):2347-2358. Epub 2021 Sep 29.

The Center for Microbes, Development, and Health, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China;

Lipid droplets (LDs), the highly dynamic intracellular organelles, are critical for lipid metabolism. Dynamic alterations in the configurations and functions of LDs during innate immune responses to bacterial infections and the underlying mechanisms, however, remain largely unknown. In this study, we trace the time-course morphology of LDs in fat bodies of after transient bacterial infection. Detailed analysis shows that (), a core gene involved in the regulation of LDs, is suppressed by the immune deficiency signaling, one major innate immune pathway in During immune activation, downregulated promotes the enlargement of LDs, which in turn alleviates immune reaction-associated reactive oxygen species stress. Thus, the growth of LDs is likely an active adaptation to maintain redox homeostasis in response to immune deficiency activation. Therefore, our study provides evidence that serves as a modulator on LDs' reconfiguration in regulating infection-induced pathogenesis, and plin1 might be a potential therapeutic target for coordinating inflammation resolution and lipid metabolism.
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http://dx.doi.org/10.4049/jimmunol.2100343DOI Listing
November 2021

Skin necrosis due to post-treatment care failure after photodynamic therapy of facial port-wine stain in Sturge-Weber Syndrome - A case report.

Photodiagnosis Photodyn Ther 2021 Sep 22:102546. Epub 2021 Sep 22.

Department of Plastic and Reconstructive Surgery, Zhejiang Provincial People's Hospital, affiliated of Hangzhou Medical College, Hangzhou China.

We report on a case of photodynamic therapy (PDT) of large facial port-wine stain (PWS) in 5-year-old patient with Sturge-Weber Syndrome (SWS). Improvement was achieved after the first session without severe adverse reactions, however, prolonged swelling, infection and skin necrosis occurred after the second session, mainly due to failure of post-treatment care. The case demonstrates that post-treatment care is critical for PDT of large facial PWS, particularly for SWS patients.
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http://dx.doi.org/10.1016/j.pdpdt.2021.102546DOI Listing
September 2021

Deep Learning-Based CT Radiomics for Feature Representation and Analysis of Aging Characteristics of Asian Bony Orbit.

J Craniofac Surg 2021 Sep 24. Epub 2021 Sep 24.

School of Electronics and Information, Hangzhou Dianzi University Department of Plastic and Reconstructive Surgery Department of Radiology, People's Hospital of Hangzhou Medical College, Zhejiang Provincial People's Hospital, Hangzhou, China.

Objective: This paper puts forward a new method for automatic segmentation of bony orbit as well as automatic extraction and classification of aging features of segmented orbit contour based on depth learning, with which the aging mode of bony orbit contour is preliminarily validated.

Method: Three-dimensional reconstruction was carried out by using the craniofacial Computed Tomography scanning data of 595 adult Mongolians at different ages (119 young males, 78 young females, 109 middle-aged males, 89 middle-aged females, 95 elderly males, and 105 elderly females), the craniofacial images were exported, orbit contour images were obtained with U-Net segmentation network, and then the orbit contour features of young group, the middle-aged group and the elderly group were classified with the classification network. Next, contour area, height, and other features put forward in existing research were automatically calculated by using the connected component shape description method; and it was validated whether the aging features of the bony orbit only occur to partial or the whole orbit.

Results: With the method put forward in this paper, high-precision identification (97.94% and 99.18%) of 3 categories in the male and female group experiments. In the meanwhile, it was found in the comparison experiment with other features that bony orbit contour definitely has features relating to aging, but these features only occur to partial areas of the orbit, which enables the convolutional neural network to achieve good identification effects. And, bone resorption of the superior orbital rim of males is more obvious than that of the inferior orbital rim, but the overall shape features like the bony orbit area and height do not change significantly along with the increase of the age.

Conclusions: U-Net can realize high-precision segmentation of the orbit contour, and with the Convolutional Neural Network-based orbit contour sorting algorithm, the aging degree of the bony orbit can be identified precisely. It is preliminarily validated that the aging mode of Mongolian bony orbit contour is that the bone resorption of the superior orbital rim is more obvious than that of the inferior orbital rim, and the change of the orbit area, perimeter, height and circularity is not obvious in the aging process.
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http://dx.doi.org/10.1097/SCS.0000000000008198DOI Listing
September 2021

Preparation of core-shell microporous organic polymer-coated silica microspheres for chromatographic separation and N-glycopeptides enrichment.

J Sep Sci 2021 Sep 24. Epub 2021 Sep 24.

Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Dalian, P. R. China.

Through a "one-pot" strategy, a layer of microporous organic polymer was coated onto the surface of monodisperse amino-functionalized silica microsphere via amino-aldehyde condensation reaction with core-shell structure. The change in chemical structure of material before and after modification was determined by Fourier-transform infrared spectroscopy and X-ray photoelectron spectroscopy. Due to existence of a large number of amino and aldehyde groups in microporous organic polymer shell, the water contact angle decreased from 56.8° (silica microspheres) to 34.7° (microporous organic polymer-coated silica microspheres). Based on these properties, microporous organic polymer-coated silica microspheres were employed as the stationary phase for capillary liquid chromatography and successfully offered baseline separation of polar small molecules. Additionally, the material could also be served as the sorbent of hydrophilic interaction chromatography to enrich glycopeptides from human serum digest. A total of 470 unique N-glycopeptides and 342 N-glycosylation sites mapped to 112 N-glycosylated proteins were unambiguously identified from 2 μL of human serum, exhibiting a promising application prospect of microporous organic polymer-coated silica microspheres in the pretreatment of proteomics samples.
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http://dx.doi.org/10.1002/jssc.202100466DOI Listing
September 2021

Long-term drench of exopolysaccharide from Leuconostoc pseudomesenteroides XG5 protects against type 1 diabetes of NOD mice via stimulating GLP-1 secretion.

J Sci Food Agric 2021 Sep 23. Epub 2021 Sep 23.

School of Chemical Engineering and Technology, Tianjin University, 135 Yaguan Road, Tianjin, 300350, China.

Background: Type 1 diabetes is an autoimmune disease that results in the specific destruction of insulin-producing beta cells in the pancreas. The aim of this study was to investigate the mechanism of exopolysaccharide from Leuconostoc pseudomesenteroides XG5 (XG5 EPS) against type 1 diabetes.

Results: Long-term drench of XG5 EPS delayed the onset of autoimmune diabetes and had fewer islets with high-grade infiltration (an insulitis score of 3 or 4) than untreated NOD mice. Oral administration of 50 mg kg  d XG5 EPS increased the insulin and glucagon-like peptide-1 (GLP-1) levels of serum, stimulated GLP-1 secretion and upregulated gcg mRNA expression of colon in NOD mice. Moreover, oral administration of 50 mg kg  d XG5 EPS significantly increased total short-chain fatty acids levels in the colon contents, especially those of acetic acid and butyric acid. In NCI-H716 cells, 500 and 1000 μmol L sodium butyrate promoted the secretion of GLP-1 and upregulated the mRNA expression of gcg and PC3, while XG5 EPS and sodium acetate did not stimulate the GLP-1 secretion. Therefore, long-term drench of XG5 EPS delayed the onset of autoimmune diabetes, which may be directly correlated with the increase of butyrate in the colon of NOD mice.

Conclusion: Long-term drench of 50 mg kg  d XG5 EPS promoted the expression and secretion of GLP-1 by increasing the production of butyric acid, thereby delaying T1D onset in NOD mice. © 2021 Society of Chemical Industry.
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http://dx.doi.org/10.1002/jsfa.11541DOI Listing
September 2021

Fabrication of hydrophilic zwitterionic microspheres via inverse suspension polymerization for the enrichment of N-glycopeptides.

Mikrochim Acta 2021 09 20;188(10):348. Epub 2021 Sep 20.

CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China.

A kind of zwitterionic microsphere was prepared via one-step inverse suspension polymerization employing 3-[N,N-dimethyl-[2-(2-methylpropyl-2-enyloxy) ethyl] ammonium] propane-1-sulfonate (MSA) and N,N-methylene bisacrylamide (BIS) as the precursors. The preparation conditions were carefully investigated and optimized by regulating the content of total monomers, ratio of MSA to BIS, ratio of water to oil, and content of stabilizer. The properties of microspheres were characterized by helium ion microscopy (HIM), Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), N adsorption/desorption measurement, and water contact angle measurement. The particle size of resulting polydisperse microspheres ranged from 15-25 μm, exhibiting high specific surface area of 138 m g. Owing to great hydrophilicity, the resulting zwitterionic microspheres could be directly used as hydrophilic interaction chromatography (HILIC) sorbent to enrich glycopeptides from biosamples without any chemical modification. A total of 19 N-glycopeptides was enriched from 10 μg of IgG digest. Besides, up to 383 N-glycopeptides and 224 N-glycosylation sites were unambiguously identified from 2 μL of human serum digest by cLC-MS/MS after enrichment with zwitterionic microspheres, indicating their great enrichment performance to N-glycopeptides. The approach of preparing hydrophilic zwitterionic microspheres contains only one synthesis reaction and is suitable for large-scale preparation.
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http://dx.doi.org/10.1007/s00604-021-05010-wDOI Listing
September 2021

Progressive disfiguring facial masses with pupillary axis obstruction from Morbihan syndrome: A case report.

World J Clin Cases 2021 Aug;9(24):7163-7168

Department of Plastic and Reconstructive Surgery, Plastic and Reconstructive Surgery Center, Zhejiang Provincial People's Hospital, Affiliated Hospital of Hangzhou Medical College, Hangzhou 310014, Zhejiang Province, China.

Background: Morbihan syndrome is a rare illness, which presents with recurrent pitting edema on the upper two thirds of the face causing facial contour deformities, and Morbihan syndrome obstructing the pupillary axis with ptosis was seldomly reported.

Case Summary: A 59-year-old woman presented with a 15-year history of facial swelling that progressively invaded bilaterally from the inner canthus and eyelids outwards. Imaging examination indicated that the bilateral periorbital and the left temporal soft tissues had swelling without cranium invasion. Histopathological analysis showed a large amount of lymphocyte infiltration, and immunohistochemistry showed positive expression of CD68 in clear-cut granulomas and D2-40 in the lymphatic endothelium. Finally, the clinical diagnosis of Morbihan syndrome was confirmed.

Conclusion: Morbihan syndrome is an infrequent and refractory disease, which is characteristic with recurrent woody facial edema on the upper two thirds of the face. Solid facial edema is persistent and non-pitting, causing facial contour deformities and even vision field impairment. The diagnosis of Morbihan syndrome depends on clinical features, imaging information, and pathology. Blepharoplasty is optional to improve the visual field for these patients with severe pupillary axis obstruction.
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http://dx.doi.org/10.12998/wjcc.v9.i24.7163DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8409198PMC
August 2021

Molecular dynamics investigation of structure evolution and thermodynamics of Ni-Fe nanoparticles during inert gas condensation.

J Mol Model 2021 Sep 18;27(10):288. Epub 2021 Sep 18.

School of Materials Science and Engineering, Research Center for Materials Genome Engineering, Wuhan University of Technology, Wuhan, 430070, China.

Synthesis of magnetic nanoparticles is relevant to many applications in the fields of catalysis, energy storage, and biomedicine. Understanding the growth mechanisms and morphology of nanoparticles during inert gas condensation is crucial to rationally improve the performance of the final nanoparticles. In this work, molecular dynamics simulations are carried out to study the structural and thermodynamic behavior of Ni-Fe nanoparticles from homogenous vapor phase in Ar atmosphere. It is revealed that the final morphology of the resulting nanoparticles presents a spherical shape by cluster coalescence at high temperatures where the small clusters are liquid droplets prior to their collisions. However, probabilistic nucleation and cluster growth indicate that the occurrence of spherical shape is more controlled by the probability limits for different Fe concentrations. Meanwhile, a larger inert gas density induces a more efficient cooling effect leading to a larger probability control of the cluster formation with non-spherical shape by agglomeration. Furthermore, the solidification of the as-formed Ni-Fe clusters is examined by evaluating the evolution of crystalline and amorphous structure. The linear scaling-down dependence of the solidification temperature on the reciprocal of the nanoparticle size clearly signifies a linear size-depression effect for the liquid-to-solid phase change of Ni-Fe nanoparticles. Our findings thus extend the current understanding of inert gas condensation behavior and mechanisms of Ni-Fe nanoparticles from an atomic/molecular perspective.
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http://dx.doi.org/10.1007/s00894-021-04908-9DOI Listing
September 2021

Molecular dynamics investigation of structural and mechanical properties of silica nanorod reinforced dental resin composites.

J Mech Behav Biomed Mater 2021 Dec 9;124:104830. Epub 2021 Sep 9.

State Key Laboratory of Materials Processing and Die & Mould Technology, Huazhong University of Science and Technology, Wuhan, 430074, China.

In this work, molecular dynamics simulations are conducted to investigate the structural and mechanical properties of dental materials, i.e., the silica nanorod reinforced Bis-GMA/TEGDMA resin composite. The effects of loading content and size of the silica nanorods on the composite stiffness were performed by examining resin chain conformation, hydrogen bonds and matrix/filler binding energy. It is revealed that the presence of the silica nanorod causes polymer chain expansion, endowing the resins with higher stiffness. Moreover, the volumetric hydrogen bonds and binding energy increase considerably with the loading content, but decrease gradually with the diameter or show almost independence of the length. Furthermore, the composite moduli were quantified by the micromechanics models and the transverse moduli were well predicted by the Counto model, signifying a perfect bonding between the matrix and nanorod. The chain expansion and energetic matrix/filler interactions are believed to contribute to the significant mechanical reinforcement of the composites with the loading content. However, the length of the nanorod has a little effect on the composite moduli due to the unaltered interfacial interaction. In contrast, a smaller diameter is supposed to give a larger modulus, and this is not observed in this work due to the synergic effects of improved matrix/filler interaction and actual reduced filler volume fraction. The mechanical enhancement by the rod-like structures is more influenced by the loading content, but less so by the size of the nanorod, and it also exhibits superior mechanical performance as compared to nanoparticles. The findings thus extend the current understanding of the nanostructure and mechanical properties of silica nanorod reinforced dental resin composites from an atomic/molecular perspective.
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http://dx.doi.org/10.1016/j.jmbbm.2021.104830DOI Listing
December 2021

Determination of X-ray detection limit and applications in perovskite X-ray detectors.

Nat Commun 2021 Sep 6;12(1):5258. Epub 2021 Sep 6.

Nuclear Engineering Program, Department of Mechanical and Aerospace Engineering, The Ohio State University, Columbus, OH, USA.

X-ray detection limit and sensitivity are important figure of merits for perovskite X-ray detectors, but literatures lack a valid mathematic expression for determining the lower limit of detection for a perovskite X-ray detector. In this work, we present a thorough analysis and new method for X-ray detection limit determination based on a statistical model that correlates the dark current and the X-ray induced photocurrent with the detection limit. The detection limit can be calculated through the measurement of dark current and sensitivity with an easy-to-follow practice. Alternatively, the detection limit may also be obtained by the measurement of dark current and photocurrent when repeatedly lowering the X-ray dose rate. While the material quality is critical, we show that the device architecture and working mode also have a significant influence on the sensitivity and the detection limit. Our work establishes a fair comparison metrics for material and detector development.
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http://dx.doi.org/10.1038/s41467-021-25648-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8421435PMC
September 2021

Stability of Time-Reversal Symmetry Protected Topological Phases.

Phys Rev Lett 2021 Aug;127(8):086801

Institute for Advanced Study, Tsinghua University, Beijing 100084, China.

In a closed system, it is well known that the time-reversal symmetry can lead to Kramers degeneracy and protect nontrivial topological states such as the quantum spin Hall insulator. In this Letter, we address the issue of whether these effects are stable against coupling to the environment, provided that both the environment and the coupling to the environment also respect time-reversal symmetry. By employing a non-Hermitian Hamiltonian with the Langevin noise term and utilizing the non-Hermitian linear response theory, we show that the spectral functions for Kramers degenerate states can be split by dissipation, and the backscattering between counterpropagating edge states can be induced by dissipation. The latter leads to the absence of accurate quantization of conductance in the case of the quantum spin Hall effect. As an example, we demonstrate this concretely with the Kane-Mele model. Our study can also include interacting topological phases protected by time-reversal symmetry.
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http://dx.doi.org/10.1103/PhysRevLett.127.086801DOI Listing
August 2021

Salidroside Suppresses the Proliferation and Migration of Human Lung Cancer Cells through AMPK-Dependent NLRP3 Inflammasome Regulation.

Oxid Med Cell Longev 2021 19;2021:6614574. Epub 2021 Aug 19.

Institute of Wudang Traditional Chinese Medicine, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, China.

Inflammatory reactions mediated by the NACHT, LRR, and PYD domain-containing protein 3 (NLRP3) inflammasome contributes to non-small-cell lung cancer (NSCLC) progression, particularly in patients with bacterial infections. Salidroside (SAL) has recently been shown to suppress lipopolysaccharide- (LPS-) induced NSCLC proliferation and migration, but its mechanism of action remains unclear. It has been shown that SAL improves metabolic inflammation in diabetic rodents through AMP-activated protein kinase- (AMPK-) dependent inhibition of the NLRP3 inflammasome. However, whether the NLRP3 inflammasome is regulated by SAL in NSCLC cells and how its underlying mechanism(s) can be determined require clarification. In this study, human lung alveolar basal carcinoma epithelial (A549) cells were treated with LPS, and the effects of SAL on cell proliferation, migration, AMPK activity, reactive oxygen species (ROS) production, and NLRP3 inflammasome activation were investigated. We found that LPS induction increases the proliferation and migration of A549 cells which was suppressed by SAL. Moreover, SAL protected A549 cells against LPS-induced AMPK inhibition, ROS production, and NLRP3 inflammasome activation. Blocking AMPK using Compound C almost completely suppressed the beneficial effects of SAL. In summary, these results indicate that SAL suppresses the proliferation and migration of human lung cancer cells through AMPK-dependent NLRP3 inflammasome regulation.
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http://dx.doi.org/10.1155/2021/6614574DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8390167PMC
September 2021

3D-printed smartphone-based device for fluorimetric diagnosis of ketosis by acetone-responsive dye marker and red emissive carbon dots.

Mikrochim Acta 2021 Aug 27;188(9):306. Epub 2021 Aug 27.

Institute of Solid State Physics, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, 230031, Anhui, China.

A portable smartphone device is reported that uses 3D printing technology for the primary diagnosis of diseases by detecting acetone. The key part of the device consists of red carbon dots (RCDs), which are used as internal standards, and a sensing reagent (3-N,N-(diacethydrazide)-9-ethylcarbazole (2-HCA)) for acetone. With an excitation wavelength of 360 nm, the emission wavelengths of 2-HCA and RCDs are 443 nm and 619 nm, respectively. 2-HCA effectively captures acetone to form a nonfluorescent acylhydrazone via a condensation reaction occurring in aqueous solution, resulting in obvious color changes from blue-violet to dark red. The detection limit for acetone is 2.62 μM (~ 0.24 ppm). This is far lower than the ketone content in normal human blood (≤ 0.50 mM) and the acetone content in human respiratory gas (≤ 1.80 ppm). The device has good recovery rates for acetone detection in blood and exhaled breath, which are 90.56-109.98% (RSD ≤ 5.48) and 92.80-108.00% (RSD ≤ 5.07), respectively. The method designed here provides a reliable way to provide health warnings by visually detecting markers of ketosis/diabetes in blood or exhaled breath. The portable smart phone device visually detects ketosis/diabetes markers in the blood or exhaled breath through the nucleophilic addition reaction, which effectively captures acetone to form nonfluorescent acyl groups. This will be a reliable tool to warn human health.
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http://dx.doi.org/10.1007/s00604-021-04965-0DOI Listing
August 2021

Ammonia exposure causes the imbalance of the gut-brain axis by altering gene networks associated with oxidative metabolism, inflammation and apoptosis.

Ecotoxicol Environ Saf 2021 Aug 25;224:112668. Epub 2021 Aug 25.

College of Life Science, Northeast Agricultural University, Harbin 150030, People's Republic ofChina; College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, People's Republic of China; Key Laboratory of Swine Facilities Engineering, Ministry of Agriculture and Rural Affairs, Harbin, People's Republic of China. Electronic address:

Ammonia is an acknowledged environment pollutant in atmosphere with irritating smell. Previous studies have shown that excessive ammonia has toxic effects on farm animals and humans. However, the detail toxicity mechanism of ammonia to pigs is still unknown so far. In order to clarify the mechanism of ammonia toxicity, we established a porcine exogenous ammonia poisoning model and assessed the effects of ammonia on the gut-brain axis by transcriptome sequencing, histological observation and chemical analysis. Our results showed that after 30 d of ammonia exposure, 578 differentially expressed genes (DEGs) and 407 DEGs were obtained in the hypothalamus and jejunum, respectively. These DEGs were enriched into Gene Ontology terms associated with inflammation, oxidative metabolism, apoptosis, and the highly expressed genes among these DEGs were verified by real-time quantitative PCR. The content of glutathione and the activities of glutathione peroxidase and superoxide dismutase were significantly decreased, while malondialdehyde content was increased after ammonia exposure. Corticotropin releasing factor, substance P, 5-hydroxytryptamine and ghrelin contents in serum elevated significantly. Furthermore, pathologic observation in the ammonia group revealed infiltration of lymphocytes in the hypothalamus and significant decrease of jejunal epithelial cells. Our results indicated that ammonia exposure mediated changes in transcriptional profiles, pathological damage, oxidative stress and brain-gut peptide of the pig jejunum and hypothalamus, and induced the imbalance of the brain-gut axis through the "oxidative stress-inflammation-apoptosis" interaction network. Our study not only provides a new perspective for the toxicity assessment of ammonia, but also enriches the toxicology mechanism of ammonia.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112668DOI Listing
August 2021

PrbP modulates biofilm formation in Liberibacter crescens.

Environ Microbiol 2021 Aug 25. Epub 2021 Aug 25.

Microbiology and Cell Science Department, Genetics Institute, Institute of Food and Agricultural Science, University of Florida, Gainesville, FL, USA.

In Liberibacter asiaticus, PrbP is a transcriptional regulatory protein involved in survival and persistence during host infection. Tolfenamic acid was previously found to inhibit interactions between PrbP and the promotor region of rplK, resulting in reduced survival of L. asiaticus in the citrus host. In this study, we performed transcriptome analyses to elucidate the PrbP regulon in L. crescens, as it is phylogenetically the closest related species to L. asiaticus that can be grown in laboratory conditions. Chemical inhibition of PrbP with tolfenamic acid revealed that PrbP is involved in the regulation of diverse cellular processes, including stress response, cell motility, cell cycle and biofilm formation. In vitro DNA binding and bacterial two-hybrid assays also suggested that PrbP is a global regulator of multiple transcription factors (RpoH, VisN, PleD, MucR, MocR and CtrA) at both transcriptional and/or post-transcriptional levels. Sub-lethal concentrations of tolfenamic acid significantly reduced the attachment of L. crescens during biofilm formation and decreased long-term persistence in biofilm structures. Overall, our findings show the importance of PrbP in regulating diverse biological processes through direct and indirect interactions with other transcriptional regulators in L. crescens.
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http://dx.doi.org/10.1111/1462-2920.15740DOI Listing
August 2021

Berberine Reshapes the Balance of the Local Renin-Angiotensin System by Modulating Autophagy under Metabolic Stress in Pancreatic Islets.

J Renin Angiotensin Aldosterone Syst 2021 3;2021:9928986. Epub 2021 Aug 3.

Department of Endocrinology, Taihe Hospital, Hubei University of Medical, Shiyan, 442000 Hubei, China.

Results: Prolonged exposure to palmitate increased the expression of ACE and AngII type 1 receptor (ATR1) and decreased the ACE2 expression, which was partly offset by berberine. In ob/ob mice, berberine increased in tolerance to glucose, improved abnormal -cell and -cell distributions, upregulated ACE2 expression, and decreased autophagosomes and the expression of LC3 and SQSTM1/p62. Autophagosomes and expression of LC3 and SQSTM1/p62 were increased in ACE2KO mice.

Conclusions: We demonstrated that berberine may improve the pancreatic islet function by regulating local RAS-mediated autophagy under metabolic stress.
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http://dx.doi.org/10.1155/2021/9928986DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8356011PMC
August 2021

Fine Mapping of the Gene Controlling the Fruit Skin Hairiness of and Its Uses for MAS in Progenies.

Plants (Basel) 2021 Jul 14;10(7). Epub 2021 Jul 14.

National Peach and Grape Improvement Center, Key Laboratory of Fruit Breeding Technology of Ministry of Agriculture, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, China.

The fruit skin pubescence of is an economically important characteristic and comprises the classification criteria. The mapping and identification of a complete linkage marker to the fruit skin trichome trait locus of peach fruit are critical for the molecular marker-assisted selection for peach/nectarine. In this study, the BC population was constructed from the parents "Zhongyou No. 4", the recurrent parent, and "Baihuashanbitao", the non-recurrent parent. Based on the 38 BC individuals' phenotypes and their genotyping using next-generation sequencing, the (Glabrous skin) locus of the gene was first identified between 14.099 and 16.721 Mb on chromosome 5. Using other individuals of this population, the gene was fine-mapped in the range of 481 kb with SNP markers. Based on the resequencing data of other cultivars (lines), the candidate SNP in the gene Prupe.5G196400 was obtained. Subsequently, the SNP marker was designed and applied to natural and hybrid peach populations. Via genotyping analysis, we confirmed co-segregation between the peach/nectarine phenotype, which was used in the identification of peach or nectarine with 100% accuracy.
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http://dx.doi.org/10.3390/plants10071433DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8309289PMC
July 2021

[Effect of Petroleum Ether Extract of Rhizoma Amorphophalli on Biological Characteristics of K562 Cells and Its Mechanism].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2021 Aug;29(4):1028-1033

Key Laboratory of Bone Arthromyodynia, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou 310006,Zhejiang Province, China,Email:

Objective: To investigate the role of petroleum ether extract of Rhizoma Amorphophalli (SLG) in inhibiting proliferation and promoting apoptosis and differentiation of leukemia K562 cells.

Methods: K562 cells were processed by SLG and PD98059 which was the ERK signaling pathway blocker. Then cell vitality was tested by MTT. Cell apoptosis rate and positive percentage of antigen expression related with differentiation were detected by flow cytometry. The protein expression levels of ERK1/2 and pERK1/2 were detected by Western blot.

Results: The proliferation activity of K562 was reduced by 50, 100, 200 mg/L SLG in a concentration dependent manner (r=0.9997). The apoptosis rate and positive expression rate of CD11b, CD14 and CD42b which were related with differentiation were raised by SLG, as well as the expression of pERK1/2, while PD98059 could reverse the promoting effect of SLG on apoptosis and differentiation partially.

Conclusion: SLG can inhibit the proliferation and promote apoptosis and differentiation of K562 cells through ERK signaling pathway.
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http://dx.doi.org/10.19746/j.cnki.issn.1009-2137.2021.04.003DOI Listing
August 2021

Misdiagnosed tuberculosis being corrected as Nocardia farcinica infection by metagenomic sequencing: a case report.

BMC Infect Dis 2021 Aug 4;21(1):754. Epub 2021 Aug 4.

Department of Tuberculosis Intensive Care Unit, Affiliated Hangzhou Chest Hospital, Zhejiang University School of Medicine, No. 208 Huancheng Dong Road, Hangzhou, 310003, China.

Background: Disseminated nocardiosis is liable to be misdiagnosed owing to the non-specific clinical manifestations and laboratory/imaging findings. Metagenomic next-generation sequencing (mNGS) is a culture-independent and rapid method for direct identification of all microorganisms in clinical specimens.

Case Presentation: A 72-year-old man was admitted to our hospital on February 20, 2019 with a history of recurrent cough, expectoration, fever, and diarrhea since 1 month, and unconsciousness since 1 week. Contrast-enhanced magnetic resonance imaging of head showed multiple lesions in the bilateral cerebral hemispheres, brainstem, and cerebellar hemispheres. The presumptive diagnosis was disseminated tuberculosis, although all tests for mycobacterium were negative. However, the patient did not benefit from antituberculosis treatment. Repeat MRI showed multiple abnormal signals in the brain and progression of meningeal thickening. Cerebrospinal fluid and bronchoalveolar lavage fluid specimens were subsequently sent for PMSeq metagenomics sequencing; the results indicated Nocardia. farcinica as the predominant pathogen. The anti-TB treatment was stopped and the patient was prescribed sulphamethoxazole in combination with linezolid and meropenem for nocardiosis. He showed gradual neurological improvement and was transferred to Huashan Hospital. He was discharged from the hospital on April 19, 2019, but died of persistent diarrhea on May 26, 2019.

Conclusions: Patients with suspected nocardiosis do not always respond to conventional treatment; therefore, mNGS can facilitate diagnosis and timely treatment decision-making.
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http://dx.doi.org/10.1186/s12879-021-06436-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8335456PMC
August 2021

Hydroxyl Radical Generation Through the Fenton-Like Reaction of Hematin- and Catechol-Functionalized Microgels.

Chem Mater 2020 Oct 4;32(19):8182-8194. Epub 2020 Sep 4.

Department of Biomedical Engineering, Michigan Technological University, Houghton, MI 49931, USA.

Hydroxyl radical (OH) is a potent reactive oxygen species with the ability to degrade hazardous organic compounds, kill bacteria, and inactivate viruses. However, an off-the-shelf, portable, and easily activated biomaterial for generating OH does not exist. Here, microgels were functionalized with catechol, an adhesive moiety found in mussel adhesive proteins, and hematin (HEM), a hydroxylated Fe ion-containing porphyrin derivative. When the microgel was hydrated in an aqueous solution with physiological pH, molecular oxygen in the solution oxidized catechol to generate HO, which was further converted to OH by HEM. The generated OH was able to degrade organic dyes, including orange II and malachite green. Additionally, the generated OH was antimicrobial against both gram-negative () and gram-positive () bacteria with the initial concentration of 10-10 CFU/mL. These microgels also reduced the infectivity of a non-enveloped porcine parvovirus and an enveloped bovine viral diarrhea virus by 3.5 and 4.5 log reduction values, respectively (99.97-99.997% reduction in infectivity). These microgels were also functionalized with positively charged [2-(methacryloyloxy)ethyl] trimethylammonium chloride (METAC), which significantly enhanced the antibacterial and antiviral activities through electrostatic interaction between the negatively charged pathogens and the microgel. These microgels can potentially serve as a lightweight and portable source of disinfectant, for an on-demand generation of OH with a wide range of applications.
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http://dx.doi.org/10.1021/acs.chemmater.0c01551DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8323869PMC
October 2020

Premolar enamel thickness and distribution of a Miocene hominid Lufengpithecus hudienensis compared with Pleistocene and extant hominids.

J Hum Evol 2021 08 15;157:103030. Epub 2021 Jul 15.

Key Laboratory of Vertebrate Evolution and Human Origins, Institute of Vertebrate Paleontology and Paleoanthropology, CAS, Beijing, China; CAS Center for Excellence in Life and Paleoenvironment, Beijing, China; Centro Nacional de Investigación sobre la Evolución Humana, Paseo de la Sierra de Atapuerca s/n, Burgos, Spain.

Lufengpithecus hudienensis is a Late-Miocene hominid from the Yuanmou basin of southwestern China. Previous studies link Lufengpithecus to either the Sivapithecus-orangutan clade or a derived branch from the basal stem of the Hominidae. Despite a rich fossil assemblage, the taxonomy of L. hudienensis and its phylogenetic relationship with other hominids is still unclear. Enamel thickness in fossil and modern hominids can provide insights into taxonomy, phylogeny, and dietary reconstructions. In this study, 24 upper and lower L. hudienensis premolars were imaged using high-resolution microcomputed tomography. Three-dimensional average enamel thickness and relative enamel thickness (RET) indices, as well as whole-crown enamel distribution patterns were recorded for the L. hudienensis specimens and compared with a total of 113 specimens of fossil and extant apes and recent modern humans (RMH): fossil Pongo (n = 36), Gigantopithecus blacki (n = 21), Pan troglodytes (n = 11), Gorilla gorilla (n = 7), and RMH (n = 38). RET of the premolars of L. hudienensis was found to be similar to that of fossil Pongo, P. troglodytes, and G. gorilla, but less than Gi. blacki and RMH values. The distribution of enamel thickness shows a taxon-specific pattern for L. hudienensis, generally characterized by thicker enamel on the lateral wall than on the cusp apex, on the lingual cusp of the upper and buccal cusp of the lower premolars, with some differences with respect to fossil Pongo, Gi. blacki, P. troglodytes, and G. gorilla patterns. Additional characterizations of enamel thickness distribution patterns of the molars and other dental endostructural morphologies are needed to further explore the phylogenetic relationships of L. hudienensis with other hominids.
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http://dx.doi.org/10.1016/j.jhevol.2021.103030DOI Listing
August 2021

The Relationship Between Preoperative Systemic Immune Inflammation Index and Prognostic Nutritional Index and the Prognosis of Patients With Alveolar Hydatid Disease.

Front Immunol 2021 24;12:691364. Epub 2021 Jun 24.

Department of Hepatopancreatobiliary Surgery, Affiliated Hospital of Qinghai University, Xining, China.

Background: To explore the relationship between the preoperative immune inflammation index (SII) and the prognostic nutritional index (PNI) and the overall survival rate (OS) of patients with alveolar hydatid disease.

Methods: The clinical data of patients with hepatic alveolar echinococcosis treated by surgery in the Department of Hepatobiliary and Pancreatic Surgery, Affiliated Hospital of Qinghai University from January 2015 to January 2019 were analyzed retrospectively, and the SII, PNI, PLR and NLR were calculated. Spearman correlation analysis was utilized to analyze the correlation among SII, PNI, PLR and NLR. Receiver operating characteristic curve (ROC) was utilized to determine the best intercept values of SII, PNI, PLR and NLR, and Chi-square test was used to evaluate the relationship between SII, PNI and various clinicopathological features in patients with hepatic alveolar echinococcosis. The kaplan-Meier method was used to draw survival curves and analyze the relationship between them and the total survival time of patients. A cox regression model was used to analyze the relationship between SII, PNI and the prognosis of patients with hepatic alveolar echinococcosis. Finally, ROC curve was used to estimate the predictive efficacy of SII, PNI and COSII-PNI for the prognosis of patients with hepatic alveolar echinococcosis.

Results: A total of 242 patients were included, including 96 males and 146 females, aged 11.0-67.0 (36.6 ± 11.7) years. The values of SII, PNI, PLR and NLR are calculated, and the best truncation values of SII, PNI, PLR and NLR are given in ROC curve. The kaplan-Meier survival curve was used to analyze the relationship between SII, PNI, PLR, NLR and the overall survival time of patients with hepatic alveolar echinococcosis. The results showed that the median follow-up time was 45 months (95%CI: 39.484-50.516), and the average survival time was 49 months (95%CI: 47.300-51.931), which was low p<0.001); The 5-year OS rate of low PNI was significantly lower than that of high PNI group (37.7% 71.6%; p<0.001); The 5-year OS rate in low PLR group was significantly higher than that in high PLR group (70.4% 24.3%; p<0.001); The 5-year OS rate in low NLR group was significantly higher than that in high NLR group (67.2% 28.8%; p<0.001). Cox unifoliate analysis showed that SII, PNI, PLR and NLR were important prognostic factors related to OS. Cox multivariate analysis showed that SII(HR=4.678, 95% CI: 2.581-8.480, P<0.001) and PNI(HR=0.530, 95%CI: 0.305-0.920, P<0.05) were identified as independent risk indicators of OS, while NL was identified as independent risk indicators of OS ROC curve analysis showed that AUC of SII, PNI, PLR, NLR and COSII-PNI were 0.670(95%CI: 0.601-0.738), 0.638(95%CI: 0.561-0.716) and 0.618(95% CI: 0.541-0.694), respectively COSII-PNI is superior to SII and PNI in evaluating prognosis (P < 0.05).

Conclusions: SII and PNI can be regarded as independent risk factors reflecting the prognosis of patients with hepatic alveolar echinococcosis. The lower SII and the higher PNI before operation, the better the prognosis of patients, and the combined application of SII and PNI before operation can improve the accuracy of prediction.
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http://dx.doi.org/10.3389/fimmu.2021.691364DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8264590PMC
June 2021

Expression and prognostic analyses of HDACs in human gastric cancer based on bioinformatic analysis.

Medicine (Baltimore) 2021 Jul;100(27):e26554

Department of First Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou.

Abstract: Gastric cancer (GC) is a common cancerous tumor, and is the third leading cause of cancer mortality worldwide. Although comprehensive therapies of GC have been widely used in clinical set ups, advanced gastric cancer carries is characterized by poor prognosis, probably due to lack of effective prognostic biomarkers. Mammalian histone deacetylase family, histone deacetylases (HDACs), play significant roles in initiation and progression of tumors. Aberrant expression of HDACs is reported in many cancer types including gastric cancer, and may serve as candidate biomarkers or therapeutic targets for GC patients.Gene Expression Profiling Interactive Analysis was used to explore mRNA levels of HDACs in GC. Kaplan-Meier plotter was used to determine the prognostic value of HDACs mRNA expression in GC. Genomic profiles including mutations of HDACs were retrieved from cBioPortal webserver. A protein-protein interaction network was constructed using STRING database. GeneMANIA was used to retrieve additional genes or proteins related to HDACs. R software was used for functional enrichment analyses.Analysis of mRNA levels of HDAC1/2/4/8/9 showed that they were upregulated in GC tissues, whereas HDAC6/10 was downregulated in GC tissues. Aberrant expression of HDAC1/3/4/5/6/7/8/10/11 was all correlated with prognosis in GC. In addition, expression levels of HDACs were correlated with different Lauren classifications, and clinical stages, lymph node status, treatment, and human epidermal growth factor receptor 2 status in GC.The findings of this study showed that HDAC members are potential biomarkers for diagnosis or prognosis of gastric cancer. However, further studies should be conducted to validate these findings.
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http://dx.doi.org/10.1097/MD.0000000000026554DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8270587PMC
July 2021

Insights into Temperature and Hypoxia Tolerance in Cowpea Weevil via HIF-1.

Pathogens 2021 Jun 5;10(6). Epub 2021 Jun 5.

School of Life Sciences, Jianghan University, Wuhan 430056, China.

Cowpea weevil () is a major pest that leads to severe damage of the stored leguminous grains. Several management approaches, including physical barriers, biological or chemical methods, are used for controlling bruchid in cowpea. These methods usually target the metabolically active state of weevil. However, it becomes less effective at early stages as egg, larva, or pupa under low temperature and oxygen conditions. Since hypoxia-inducible factor-1 (HIF-1) is known to coordinate multiple gene responses to low oxygen or low temperature signals, we examined the gene expression under low temperature and hypoxic treatments. At -20 °C, it took 4 h to reduce the survival rate for eggs, larvae, and pupae down to 10%, while at 4 °C and 15 °C, the survival rate remained higher than 50% even after 128 h as gene expression peaked at 15 °C. Moreover, HIF-1 protein offers a valuable target for early stage pest control complementary to traditional methods. In particular, HIF-1 inhibitor camptothecin (CPT), one of the five HIF-1 inhibitors examined, achieved a very significant reduction of 96.2% and 95.5% relative to the control in weevil survival rate into adult at 4 °C and 30 °C, respectively. Our study can be used as one model system for drug development in virus infections and human cancer.
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http://dx.doi.org/10.3390/pathogens10060704DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8228136PMC
June 2021
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