Publications by authors named "Lei Huang"

1,806 Publications

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Discovery of the First Selective IDO2 Inhibitor As Novel Immunotherapeutic Avenues for Rheumatoid Arthritis.

J Med Chem 2022 Aug 11. Epub 2022 Aug 11.

State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China.

Indoleamine 2,3-dioxygenase 2 (IDO2), a closely related homologue of well-studied immunomodulatory enzyme IDO1, has been identified as a pathogenic mediator of inflammatory autoimmunity in preclinical models. Therapeutic targeting IDO2 in autoimmune diseases has been challenging due to the lack of small-molecule IDO2 inhibitors. Here, based on our previously developed IDO1/IDO2 dual inhibitor, guided by the homology model of the IDO2 structure, we discovered compound , the most potent inhibitor targeting IDO2 with good inhibitory activity (IDO2 IC = 112 nM). Notably, treatment with alleviated disease severity and reduced inflammatory cytokines in both the collagen-induced arthritis (CIA) mice model and adjuvant arthritis (AA) rat model. Our study offered for the first time a selective small-molecule IDO2 inhibitor with IC at the nanomolar level, which may be used not only as a candidate compound for the treatment of autoimmune diseases but also as a tool compound for further IDO2-related mechanistic study.
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http://dx.doi.org/10.1021/acs.jmedchem.2c00263DOI Listing
August 2022

A co-doped oxygen reduction catalyst with FeCu promotes the stability of microbial fuel cells.

J Colloid Interface Sci 2022 Jul 14;628(Pt A):652-662. Epub 2022 Jul 14.

Laboratory of Organic Electronics, Department of Science and Technology, Linköping University, Norrköping 60174, Sweden.

Air cathode microbial fuel cell (AC-MFC) cannot be used on a large scale because of its low oxygen reduction reaction (ORR) efficiency. Despite the fact that bimetallic catalysts can greatly enhance the oxygen reduction rate by regulating the electronic structure of the active site, the flaws of insufficient exposure of the active site and easy metal agglomeration limit its catalytic activity. Herein, we report on the preparation of a stable heteroatomic substrate using a copper material organic framework as a precursor, covered by Fe-based active sites. As a result of dipole-dipole interactions, the reduced product Fe forms a weak Fe-O surface that is conducive to the adsorption of active substances. The presence of Fe enhances the electrical conductivity of the catalytic, thus promoting ORR efficiency. Through redox coupling, the D-band center of Fe at [email protected] is optimized and brought close to the Fermi level to facilitate electron transfer. Notably, [email protected] demonstrates a superior power density of 2796.23 ± 278.58 mW m, far exceeding that of Pt/C (1363.93 ± 102.56 mW m), in the application of microbial fuel cells (MFCs). Meanwhile, the MFC-loaded [email protected] maintains excellent stability and outstanding output voltage after 1000 h, which provides feasibility for large-scale application.
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http://dx.doi.org/10.1016/j.jcis.2022.07.068DOI Listing
July 2022

Editorial: Implications of Ferroptosis-Related Genes to the Genetics of Cancer Development.

Front Genet 2022 22;13:952340. Epub 2022 Jul 22.

The Marine Biomedical Research Institute, Guangdong Medical University, Zhanjiang, China.

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http://dx.doi.org/10.3389/fgene.2022.952340DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355317PMC
July 2022

Magnitude and determinants of plant root hydraulic redistribution: A global synthesis analysis.

Front Plant Sci 2022 22;13:918585. Epub 2022 Jul 22.

Shapotou Desert Research and Experiment Station, Northwest Institute of Eco-Environmental Resources, Chinese Academy of Sciences, Lanzhou, China.

Plant root hydraulic redistribution (HR) has been widely recognized as a phenomenon that helps alleviate vegetation drought stress. However, a systematic assessment of the magnitude of HR and its drivers at the global scale are lacking. We collected 37 peer-reviewed papers (comprising 47 research sites) published in 1900-2018 and comprehensively analyzed the magnitude of HR and its underlying factors. We used a weighting method to analyze HR magnitude and its effect on plant transpiration. Machine learning algorithms (boosted regression trees) and structural equation modeling were used to determine the influence of each factor on HR magnitude. We found that the magnitude of HR was 0.249 mm HO d (95% CI, 0.113-0.384) and its contribution to plant transpiration was 27.4% (3-79%). HR varied significantly among different terrestrial biomes and mainly occurred in forests with drier conditions, such as temperate forest ecosystems (HR = 0.502 mm HO d), where HR was significantly higher than in other ecosystems ( < 0.01). The magnitude of HR in angiosperms was significantly higher than that in gymnosperms ( < 0.05). The mean magnitude of HR first increased and then decreased with an increase in humidity index; conversely, the mean magnitude of HR decreased with an increase in water table depth. HR was significantly positively correlated with root length and transpiration. Plant characteristics and environmental factors jointly accounted for 61.0% of the variation in HR, and plant transpiration was the major factor that directly influenced HR (43.1% relative importance; < 0.001), and soil texture was an important indirect driver of HR. Our synthesis offers a comprehensive perspective of how plant characteristics and environmental factors influence HR magnitude.
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http://dx.doi.org/10.3389/fpls.2022.918585DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355616PMC
July 2022

Onyx embolization of a spinal epidural hemorrhage caused by thoracic spinal epidural arteriovenous fistula: A case report and literature review.

J Interv Med 2022 May 21;5(2):111-115. Epub 2022 May 21.

Department of Radiology, Huashan Hospital, Fudan University, Shanghai, 200040, China.

Spinal epidural hemorrhages (SEDH) caused by spinal epidural arteriovenous fistulas (SEAVFs) are rare; thus, their specific pathogenesis has not been explained. Furthermore, the standard treatment for SEAVFs has not yet been defined. Here we report the case of a 36-year-old Chinese man who experienced acute onset chest pain and tightness. His symptoms rapidly aggravated until the lower limbs were unable to support him. Spinal magnetic resonance angiography (MRA) revealed a localized SEAVF and a secondary spinal cord lesion at the T4 level. Digital subtraction angiography (DSA) confirmed the presence of the SEDH/SEAVF at the T3-4 level with the left radicular artery feeding the fistula. Based on DSA and MRA findings, SEDH, local spinal cord infarction, and spinal venous reflux disorder were conditionally diagnosed. Using the arterial route, Onyx-34 was injected into the fistula to embolize the feeding arteries and the venous system. Angiography was performed after the microcatheter was withdrawn, and no residual fistula or anterior spinal artery was observed. The six-week follow-up MRI showed acceptable healing of the SEAVF, and the patient improved neurologically. This case suggests that endovascular treatment with Onyx-34 embolization should be considered a promising treatment strategy for this type of complicated SEAVF.
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http://dx.doi.org/10.1016/j.jimed.2022.03.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9349020PMC
May 2022

Structure, mechanism, and toxicity in antibiotics metal complexation: Recent advances and perspectives.

Sci Total Environ 2022 Aug 1:157778. Epub 2022 Aug 1.

Key Laboratory of the Three Gorges Reservoir Region's Eco-Environment (Ministry of Education), College of Resources and Environment, Southwest University, Chongqing 400715, PR China; Chongqing Key Laboratory of Agricultural Resources and Environment, Chongqing 400716, PR China. Electronic address:

Antibiotic-metal complexes (AMCs) formed by antibiotics and metal ions have attracted considerable attentions in recent years. Although different removal methods for AMCs have been reported in the literature, very few investigations have focused on the mechanisms and toxic effects of antibiotic-metal coordination. This review briefly describes the structural characteristics of various commonly used antibiotics and the coordination mechanisms with metal ions. Considering the complexity of the real environment, various environmental factors affecting AMC formation are highlighted. The effects of AMCs on microbial community structure and the role of metal ions in influencing resistant genes from the molecular perspective are of interest within this work. The toxicities and mechanisms of AMCs on different species of biota are also discussed. These findings underline the need for more targeted detection and analysis methods and more suitable toxicity markers to verify the combination of antibiotics with metal ions and reveal environmental toxicities in future. This review presents an innovative idea that antibiotics combined with metal ions will change the toxicity and environmental behavior of antibiotics.
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http://dx.doi.org/10.1016/j.scitotenv.2022.157778DOI Listing
August 2022

Hypersampones A-C, Three Nor-Polycyclic Polyprenylated Acylphloroglucinols with Lipid-Lowering Activity from .

Org Lett 2022 Aug 4. Epub 2022 Aug 4.

State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang 550014, China.

Hypersampones A-C (), three unprecedented nor-polycyclic polyprenylated acylphloroglucinols (PPAPs), were isolated from . These compounds represent the first nor-PPAPs with an unexpected tetracyclic 6/5/5/6 ring system. Their structures were assigned through the analysis of detailed spectroscopic data, X-ray crystallography, and electronic circular dichroism calculations. Compound significantly inhibited the accumulation of lipid in an oleic acid-treated HepG2 cell model by suppressing the protein expression of FAS and ACACA at 5 μM.
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http://dx.doi.org/10.1021/acs.orglett.2c02240DOI Listing
August 2022

Callerya atropurpurea suppresses inflammation in vitro and ameliorates gastric injury as well as septic shock in vivo via TLR4/MyD88-dependent cascade.

Phytomedicine 2022 Jul 19;105:154338. Epub 2022 Jul 19.

Department of Integrative Biotechnology and Biomedical Institute for Convergence at SKKU (BICS), Sungkyunkwan University, 2066 Seobu-ro, Jangan-gu, Suwon 16419, Republic of Korea; Department of Biocosmetics, Sungkyunkwan University, Suwon 16419, Republic of Korea. Electronic address:

Background: Callerya atropurpurea is a traditional plant in a tropical zone discovered to have anti-inflammatory functions.

Purpose: we want to investigate the mechanism related to anti-inflammation of C. atropurpurea ethanol extract (Ca-EE) both in vitro and in vivo.

Study Design: Murine macrophage cells and mouse models for gastritis and septic shock were conducted to evaluate the abilities of Ca-EE in anti-inflammation.

Methods: Ca-EE was tested by HPLC and LC-MS/MS. NO outcome was checked by Griess reagent test. Cell viabilities were evaluated using MTT assay. Inflammatory cytokines were determined via RT-PCR and ELISA. The mechanism of Ca-EE in anti-inflammation was investigated by luciferase reporter gene assay and immunoblot in transcription level and protein level respectively. Gastric injury and septic shock administrated with Ca-EE were studied by H&E, PCR, and immunoblot.

Results: Ca-EE significantly decreased LPS-induced NO production, but hardly stimulated the expression of NO itself. It not only showed no cytotoxicity, but also protected cells from LPS damage. Moreover, Ca-EE decreased TLR4 expression, altered MyD88 recruitment and TRAF6, and suppressed the phospho-Src/PI3K/AKT. Ca-EE inhibited downstream signaling P38, JNK and NF-κB. Finally, Ca-EE alleviated HCl/EtOH-induced gastritis and LPS/poly (I:C)-induced septic shock through the previously mentioned signaling cascades.

Conclusion: Ca-EE exhibited an integrated and promising mechanism against TLR4-related inflammation, which shows potential for treating gastritis, septic shock, and other inflammatory diseases.
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http://dx.doi.org/10.1016/j.phymed.2022.154338DOI Listing
July 2022

Using a two-step method of surface mechanical attrition treatment and calcium ion implantation to promote the osteogenic activity of mesenchymal stem cells as well as biomineralization on a β-titanium surface.

RSC Adv 2022 Jul 13;12(31):20037-20053. Epub 2022 Jul 13.

Department of Gastrointestinal Surgery, Hubei Cancer Hospital Wuhan 430060 China.

Surface treatment is known as a very efficient measure by which to modulate the surface properties of biomaterials in terms of grain structure, topography, roughness and chemistry to determine the osseointegration of implants. In this work, a two-step method of surface modification was employed to impart high osteogenic activity and biomineralization capacity on a Ti-25Nb-3Mo-2Sn-3Zr alloy (a type of β-titanium named TLM). The preliminary surface mechanical attrition treatment (SMAT) refined the average grain size from 170 ± 19 μm to 74 ± 8 nm in the TLM surface layer and promoted the surface to be much rougher and more hydrophilic. The subsequent Ca-ion implantation did not change the surface roughness and topography obviously, but enhanced the surface wettability of the SMAT-treated TLM alloy. The evaluations of the adhesion, proliferation, osteogenic genes (RUNX2, ALP, BMP-2, OPN, OCN and COL-I) and protein (ALP, OPN, OCN and COL-I) expressions, as well as extracellular matrix (ECM) mineralization of mesenchymal stem cells (MSCs) revealed that the initial SMAT-treated sample significantly enhanced the adhesion and osteogenic functions of MSCs compared to an untreated TLM sample, and the subsequent introduction of Ca ions onto the SMAT-derived nanograined sample further promotes the MSC adhesion, proliferation, osteo-differentiation and ECM mineralization due to the adsorption of more proteins such as laminin (Ln), fibronectin (Fn) and vitronectin (Vn) on the surface, as well as the increase in extracellular Ca concentrations. In addition, the biomineralization capacity of the samples was also evaluated by soaking them in simulated bodily fluid (SBF) at 37 °C for 28 days, and the results showed that the Ca-ion implanted sample significantly boosted the deposition of Ca and P containing minerals on its surface, which was associated with the generation of more Ti-OH groups on the surface after ion implantation. The combination of the SMAT technique and Ca-ion implantation thus endowed the TLM alloy with outstanding osteogenic and biomineralization properties, providing a potential means for its future use in the orthopedic field.
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http://dx.doi.org/10.1039/d2ra00032fDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9277716PMC
July 2022

Cyclic hexadepsipeptides from the fermentation of sp. DCJ-A and their cytotoxic activities.

J Asian Nat Prod Res 2022 Jul 31:1-7. Epub 2022 Jul 31.

Key Laboratory of Chemistry in Ethnic Medicinal Resources, State Ethnic Affairs Commission & Ministry of Education, Yunnan Minzu University, Kunming 650031, China.

Beauvercin H (), a new cyclic hexadepsipeptide, and two known ones ( and ) were isolated from the EtOH extract of the solid culture of sp. Their structures were elucidated by spectroscopic analysis, including extensive 1D and 2D NMR techniques, as well as comparison with literature values. Additionally, compounds - were tested for their cytotoxic activities. The results showed that all isolated compounds exhibited cytotoxic activities against five human cancer cell lines with IC values ranging from 1.379 to 13.12 μM.
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http://dx.doi.org/10.1080/10286020.2022.2098471DOI Listing
July 2022

Influence of Workload, Personality, and Psychological Flexibility on Occupational Stress Among Medical Staff: A Fuzzy-Set Qualitative Comparative Analysis.

Front Public Health 2022 15;10:929683. Epub 2022 Jul 15.

Department of Environmental Health and Occupational Medicine, West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, China.

Objective: During the COVID-19 pandemic, the occupational stress of medical staff has been a major issue. This study aimed to suggest a new strategy to identify high-risk factor sets of occupational stress in medical staff using fuzzy-set qualitative comparative analysis (fs-QCA) and provide ideas for the prevention and intervention of occupational stress.

Methods: A total of 1,928 medical staff members were surveyed and tested using the Acceptance and Action Questionnaire-II (AAQ-II), Occupational Stress Inventory-Revised edition (OSI-R), and Eysenck Personality Questionnaire-Revised Short Scale (EPQ-RSC). The fs-QCA was used to explore the high-risk factors for occupational stress among medical staff.

Results: The psychological strain (PSY) score of the medical staff was 26.8 ± 7.13, and the physical strain (PHS) score was 24.3 ± 6.50. Low psychological flexibility score-introversion-high role overload, introversion-neuroticism-high role overload, and low psychological flexibility score-neuroticism were high-risk factor sets for PSY. Low psychological flexibility score-introversion-high role overload, low psychological flexibility score-introversion-neuroticism, low psychological flexibility score-neuroticism-high role overload, low psychological flexibility score-psychoticism-neuroticism, and psychoticism-neuroticism-high role overload were high-risk factor sets for PHS.

Conclusion: There are different combinations of high-risk factors for occupational stress among the medical staff. For occupational stress intervention and psychological counseling, targeted and individualized health intervention measures should be implemented according to specific characteristic combinations of different individuals.
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http://dx.doi.org/10.3389/fpubh.2022.929683DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9334916PMC
August 2022

Selecting Representative Samples From Complex Biological Datasets Using K-Medoids Clustering.

Front Genet 2022 18;13:954024. Epub 2022 Jul 18.

University of Chicago Department of Medicine, Section of Rheumatology, University of Chicago, Chicago, IL, United States.

Rapid growth of single-cell sequencing techniques enables researchers to investigate almost millions of cells with diverse properties in a single experiment. Meanwhile, it also presents great challenges for selecting representative samples from massive single-cell populations for further experimental characterization, which requires a robust and compact sampling with balancing diverse properties of different priority levels. The conventional sampling methods fail to generate representative and generalizable subsets from a massive single-cell population or more complicated ensembles. Here, we present a toolkit called Cookie which can efficiently select out the most representative samples from a massive single-cell population with diverse properties. This method quantifies the relationships/similarities among samples using their Manhattan distances by vectorizing all given properties and then determines an appropriate sample size by evaluating the coverage of key properties from multiple candidate sizes, following by a k-medoids clustering to group samples into several clusters and selects centers from each cluster as the most representatives. Comparison of Cookie with conventional sampling methods using a single-cell atlas dataset, epidemiology surveillance data, and a simulated dataset shows the high efficacy, efficiency, and flexibly of Cookie. The Cookie toolkit is implemented in R and is freely available at https://wilsonimmunologylab.github.io/Cookie/.
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http://dx.doi.org/10.3389/fgene.2022.954024DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9335369PMC
July 2022

Systematic review of efficacy of direct oral anticoagulants and vitamin K antagonists in left ventricular thrombus.

ESC Heart Fail 2022 Jul 27. Epub 2022 Jul 27.

Department of Cardiology, Shengjing Hospital of China Medical University, Shenyang, China.

Aims: Left ventricular thrombus (LVT) increases the risk of thrombotic events and mortality. Vitamin K antagonists (VKAs) used to treat LVT have several known risks, as a result of which direct oral anticoagulant (DOAC) use has recently increased. We aimed to evaluate the safety and efficacy of DOACs and VKAs in treating LVT.

Methods And Results: We searched PubMed, Embase, Cochrane Library trials, and Web of Science databases for studies published before 19 April 2022, involving DOAC versus VKA treatment for patients with LVT. This meta-analysis comprised 21 studies (total patients, n = 3172; DOAC group, n = 888; VKA group, n = 2284). A statistically significant reduction in bleeding events was observed in patients on DOACs vs. those on VKAs (risk ratio (RR) = 0.73, P = 0.004). Patients on DOACs residing in North American and European regions and those with ischaemic heart disease (IHD) had a significantly lower risk of bleeding events than patients residing in other regions or those with a different LVT aetiology, respectively (RR = 0.78, P = 0.04; RR = 0.38, P = 0.02; and RR = 0.63, P = 0.009). A statistically significant reduction in stroke in patients on DOACs versus VKAs (RR = 0.72, P = 0.03) was observed, and patients on DOACs residing in North America and those with IHD had a significantly lower risk of stroke (RR = 0.73, P = 0.04, and RR = 0.61, P = 0.03, respectively). Compared with VKAs, DOACs are statistically associated with an increase in LVT resolution at 1 month (RR = 1.96, P = 0.008). No statistical between-group difference in all-cause mortality (RR = 0.72, P = 0.05), systemic embolism (RR = 0.87, P = 0.74), stroke or systemic embolism (RR = 0.90, P = 0.50), and LVT resolution at the end of follow-up (RR = 1.06, P = 0.13) was observed.

Conclusions: Compared with VKAs, DOACs significantly reduce the risk of bleeding events and stroke in LVT patients, but mortality was similar in both groups. The advantages are apparent not only in patients belonging to the predominantly white residential areas such as North American and European regions but also in patients with LVT due to IHD. DOACs show promising effects in treating LVT compared with VKAs.
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http://dx.doi.org/10.1002/ehf2.14084DOI Listing
July 2022

Natural Halloysite-Templated Synthesis of Highly Graphitic Boron-Doped Hollow Carbon Nanocapsule Webs.

Nanomaterials (Basel) 2022 Jul 9;12(14). Epub 2022 Jul 9.

School of Environmental Science and Engineering, Guangzhou University, Guangzhou 510006, China.

Hollow carbon nanocapsules have been attracting growing interest due to their fascinating characteristics and extensive potential applications. In this work, a novel natural halloysite-templated synthesis approach for highly graphitic boron-doped hollow carbon nanocapsule webs (B-HCNCWs) using glucose as the carbon source and boric acid as the heteroatom dopant was first reported. The formation process and physicochemical properties of B-HCNCWs were revealed by SEM, TEM, XRD, Raman, Brunauer-Emmett-Teller (BET), and XPS characterization techniques. The outcomes showed that the as-obtained B-HCNCWs with hollow nanocapsule network architecture had a specific surface area of 263 m g, a pore volume of 0.8 cm g, a high degree of graphitization (81.4%), graphite-like interplanar spacing (0.3370 nm), and B-containing functional groups (0.77 at%). The density function theory (DFT) calculation demonstrated that the adsorption energies of Li on B-HCNCWs were much higher than that of HCNCWs, which proved that B-doping in a carbon matrix could increase the lithium intercalation capacity.
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http://dx.doi.org/10.3390/nano12142352DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9316536PMC
July 2022

The Effects of Heat Treatment on Microstructure and Mechanical Properties of Selective Laser Melting 6061 Aluminum Alloy.

Micromachines (Basel) 2022 Jun 30;13(7). Epub 2022 Jun 30.

School of Mechanical and Electrical Engineering, Anhui Jianzhu University, Hefei 230601, China.

Selective laser melting technology can be used for forming curved panels of 6061 aluminum alloy thermal shield devices for the International Thermonuclear Experimental Reactor (ITER), in order to make the formed parts with better performance. This study proposes different heat treatment processes, including annealed treatment at 300 °C for 2 h, solution treatment at 535 °C and then aging at 175 °C over 2 h, to control the mechanical behavior of the 6061 aluminum alloy samples prepared by selective laser melting (SLM). The mechanical properties such as ductility, tensile strength, and hardness of SLM 6061 aluminum alloy were investigated, and the microstructure of the samples was analyzed. The eutectic silicon skeleton shape disappeared after annealing treatment at 300 °C for 2 h. The tensile strength decreased by 22.86% (from 315 MPa to 243 MPa of the deposited state samples), and the elongation increased from 2.01% to 6.89%. Moreover, the hardness reduced from 120.07 HV to 89.6 HV. After solution aging, the unique microstructure of SLM disappeared. Furthermore, the precipitation of massive Si particles on the α-Al matrix increased, and a trace amount of the MgSi(β) phase was generated. Compared with the deposited samples, the tensile strength decreased by 12.06%, while the hardness of specimens was 118.8 HV. However, the elongation showed a remarkable increase of 297% (from 2.01% to 7.97%). Therefore, solution aging can critically improve the plasticity without losing significant tensile stress in the SLM 6061 aluminum alloy. This study proposes the use of SLM 6061 aluminum alloy for the thermal shields on the ITER and provides a reference for choosing a reasonable heat-treatment method for the optimal performance of the SLM 6061 aluminum alloy.
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http://dx.doi.org/10.3390/mi13071059DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9324477PMC
June 2022

Puerarin Attenuates Oxidative Stress and Ferroptosis via AMPK/PGC1α/Nrf2 Pathway after Subarachnoid Hemorrhage in Rats.

Antioxidants (Basel) 2022 Jun 27;11(7). Epub 2022 Jun 27.

Department of Neurosurgery, Ningbo First Hospital, Ningbo Hospital, Zhejiang University School of Medicine, Ningbo 315010, China.

Puerarin was shown to exert anti-oxidative and anti-ferroptosis effects in multiple diseases. The goal of this study was to explore the neuroprotective effect of puerarin on early brain injury (EBI) after subarachnoid hemorrhage (SAH) in rats. A total of 177 adult male Sprague Dawley rats were used. SAH was included via endovascular perforation. Intranasal puerarin or intracerebroventricular dorsomorphin (AMPK inhibitor) and SR18292 (PGC1α inhibitor) were administered. The protein levels of pAMPK, PGC1α, Nrf2, 4HNE, HO1, MDA, ACSL4, GSSG, and iron concentration in the ipsilateral hemisphere were significantly increased, whereas SOD, GPX4, and GSH were decreased at 24 h after SAH. Moreover, puerarin treatment significantly increased the protein levels of pAMPK, PGC1α, Nrf2, HO1, SOD, GPX4, and GSH, but decreased the levels of 4HNE, MDA, ACSL4, GSSG, and iron concentration in the ipsilateral hemisphere at 24 h after SAH. Dorsomorphin or SR18292 partially abolished the beneficial effects of puerarin exerted on neurological dysfunction, oxidative stress injury, and ferroptosis. In conclusion, puerarin improved neurobehavioral impairments and attenuated oxidative-stress-induced brain ferroptosis after SAH in rats. The neuroprotection acted through the activation of the AMPK/PGC1α/Nrf2-signaling pathway. Thus, puerarin may serve as new therapeutics against EBI in SAH patients.
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http://dx.doi.org/10.3390/antiox11071259DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9312059PMC
June 2022

Soluble Receptor for Advanced Glycation End Product Is Involved in the Inflammatory Response of Human Adenovirus-Infected Patients.

Front Microbiol 2022 7;13:923215. Epub 2022 Jul 7.

Senior Department of Infectious Diseases, The Fifth Medical Center of PLA General Hospital, National Clinical Research Center for Infectious Diseases, Beijing, China.

Human adenovirus (HAdV) infection causes excessive inflammation associated with severe tissue injury, such as pneumonia. The molecules involved in the underlying inflammatory mechanisms remain to be elucidated. Receptor for advanced glycation end product (RAGE) is mainly expressed on immune cells and lung tissues, and it is a key factor in the initiation and development of inflammation. RAGE can be cleaved by metalloprotease 9 (MMP9) to release the extracellular segment, which is named soluble RAGE (sRAGE), into the intercellular space, where it can bind to RAGE ligands and block RAGE activation and subsequent inflammation. In our study, we enrolled HAdV-infected patients and their contacts to examine the relationship between sRAGE and inflammation induced by HAdV infection. The results showed that HAdV infection stimulated inflammatory cytokine secretion, increased such as high mobility group box 1 (HMGB1) levels, and suppressed sRAGE expression. sRAGE levels were significantly different between patients with or without pneumonia. We also found that MMP9 was significantly lower in patients with pneumonia, and it was positively correlated with sRAGE levels over 7 days after disease onset. The mitogen-activated protein kinase (MAPK) pathway is an important immune activation signaling pathway that is regulated by RAGE. We observed the activation of the MAPK pathway in the peripheral blood mononuclear cells (PBMCs) of patients. Negative correlations between sRAGE and phosphorylated JNK and p38 were observed. These results suggest that sRAGE is involved in HAdV-induced inflammatory responses, and might be a potential therapeutic target to alleviate the HAdV-induced excessive inflammation.
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http://dx.doi.org/10.3389/fmicb.2022.923215DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9301492PMC
July 2022

Circ_0004676 exacerbates triple-negative breast cancer progression through regulation of the miR-377-3p/E2F6/PNO1 axis.

Cell Biol Toxicol 2022 Jul 23. Epub 2022 Jul 23.

Special Medical Service Center, Zhujiang Hospital of Southern Medical University, No. 253, Middle Gongye Road, Haizhu District, Guangzhou, 510282, People's Republic of China.

Background: The significant roles of circular RNAs (circRNAs) in different cancers and diseases have been reported. We now focused on the possible role of a newly recognized circRNA, circ_0004674 in triple-negative breast cancer (TNBC), and the related downstream mechanism.

Methods: The expression of circ_0004674 in TNBC tissues and cells was determined followed by analysis of the correlation between circ_0004674 and TNBC patients' prognosis. The interaction between circ_0004674, miR-377-3p, E2F6, and PNO1 was then identified using bioinformatics analysis combined with FISH, RIP, RNA pull-down, RT-qPCR, and Western blot analysis. Using gain-of-function and loss-of-function methods, we analyzed the effect of circ_0004674, miR-377-3p, E2F6, and PNO1 on TNBC in vivo and in vitro.

Results: Increased circ_0004674 and E2F6 but decreased miR-377-3p were observed in TNBC tissues and MDA-MB-231 TNBC cells, all of which findings were associated with poor prognosis in patients with TNBC. Silencing of circ_0004676 remarkably suppressed the proliferation, cell cycle progression, and migration of TNBC cells in vitro, as well as inhibiting tumorigenesis and metastasis in vivo. Additionally, circ_0004676 served as a sponge of miR-377-3p which bound to the transcription factor E2F6. In the presence of overexpression of circ_0004676, E2F6 expression and its target PNO1 expression were elevated, while miR-377-3p expression was decreased. Interestingly, overexpression of E2F6 could reverse the inhibitory effect on tumor growth caused by downregulation of circ_0004676.

Conclusion: Our study highlighted the carcinogenic effect of circ_0004676 on TNBC through regulation of the miR-377-3p/E2F6/PNO1 axis. 1. Circ_0004674 is highly expressed in TNBC tissues and cells. 2. Circ_0004674 upregulates the expression of E2F6 by sponging miR-377-3p. 3. E2F6 upregulates PNO1 by binding to the PNO1 promoter. 4. Circ_0004674 favors TNBC progression by regulating the miR-377-3p/E2F6/PNO1 axis. 5. This study provides a new target for the treatment of TNBC.
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http://dx.doi.org/10.1007/s10565-022-09704-6DOI Listing
July 2022

Three Days Delayed Recanalization Improved Neurological Function in pMCAO Rats by Increasing M2 Microglia-Possible Involvement of the IL-4R/STAT6/PPARγ Pathway.

Transl Stroke Res 2022 Jul 22. Epub 2022 Jul 22.

Department of Physiology and Pharmacology, School of Medicine, Loma Linda University, 11041 Campus Street, Loma Linda, CA, 92354, USA.

Current approved therapies for acute ischemic stroke have a restricted therapeutic time window. Delayed recanalization, which has been utilized clinically in patients who have missed the time window for administration, may be a promising alternative for stroke patients. However, the underlying molecular mechanisms remain undiscovered. Herein, we hypothesized that delayed recanalization would increase M2 microglial polarization through the IL-4R (interleukin-4 receptor)/STAT6 (signal transducer and activators of transcription 6)/PPARγ (peroxisome proliferator-activated receptor γ) pathway, subsequently promoting stroke recovery in rats. The permanent middle cerebral artery occlusion (pMCAO) model was induced via intravascular filament insertion. Recanalization was induced by withdrawing the filament at 3 days after MCAO (rMCAO). Interleukin (IL)-4 was administered intranasally at 3 days after pMCAO. AS1517499, a specific STAT6 inhibitor, was administered intranasally at 3 days after MCAO induction. Immunofluorescence staining, enzyme-linked immunosorbent assay (ELISA), western blot analysis, volumetric measurements of brain infarct, and neurological behavior tests were conducted. Delayed recanalization at 3 days after MCAO increased the polarization of M2 microglia, decreased inflammation, and improved neurological behavior. IL-4 treatment administered on the 3rd day after pMCAO increased M2 microglial polarization, improved neurological behavior, and reduced infarction volume of pMCAO rats. The inhibition of STAT6 decreased the level of p-STAT6 and PPARγ in rats treated with delayed recanalization. Delayed recanalization improved neurological function by increasing microglial M2 polarization, possibly involved with the IL-4R/STAT6/PPARγ pathway after MCAO in rats.
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http://dx.doi.org/10.1007/s12975-022-01032-5DOI Listing
July 2022

Changes in the Mucosa-Associated Microbiome and Transcriptome across Gut Segments Are Associated with Obesity in a Metabolic Syndrome Porcine Model.

Microbiol Spectr 2022 Jul 7:e0071722. Epub 2022 Jul 7.

Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China.

Several studies have suggested a role for gut mucosa-associated microbiota in the development of obesity, but the mechanisms involved are poorly defined. Here, the impact of the gut mucosa-associated microbiota on obesity and related metabolic disorders was evaluated in a metabolic syndrome (MetS) porcine model. Body composition was determined among male Wuzhishan minipigs consuming a high-energy diet (HED) and compared to that of those consuming a normal diet (ND), and gut segments (duodenum, jejunum, ileum, cecum, colon, and rectum) were sampled for paired analysis of mucosa-associated microbiota and transcriptome signatures with 16S rRNA gene and RNA sequencing, respectively. Our data indicated that long-term HED feeding significantly increased body weight and visceral fat deposition and aggravated metabolic disorders. Specially, HED feeding induced mucosa-associated microbiota dysbiosis and selectively increased the abundance of the families , , and in the upper intestine. The association analysis indicated that specific bacteria play key roles in adiposity, e.g., Lactobacillus johnsonii in the duodenum, Actinobacillus indolicus in the jejunum, Acinetobacter johnsonii in the ileum, Clostridium butyricum in the cecum, Haemophilus parasuis in the colon, and bacterium NLAEzlP808, , and sp. JNUH029 in the rectum. Transcriptome data further revealed intestinal lipid metabolism and immune dysfunction in the MetS individuals, which may be associated with obesity and related metabolic disorders. Our results indicated that gut mucosa-associated microbiota dysbiosis has the potential to exacerbate obesity, partially through modulating systemic inflammatory responses. Obesity is a major risk factor for metabolic syndrome, which is the most common cause of death worldwide, especially in developed countries. The link between obesity and gut mucosa-associated microbiota is unclear due to challenges associated with the collection of intestinal samples from humans. The current report provides the first insight into obesity-microbiome-gut immunity connections in a metabolic syndrome (MetS) porcine model. The present results show that dysbiosis of mucosal microbiota along the entire digestive tract play a critical role in the proinflammatory response in the host-microbial metabolism axis, resulting in obesity and related metabolic disorders in the MetS model.
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http://dx.doi.org/10.1128/spectrum.00717-22DOI Listing
July 2022

Meningioma Related Epilepsy- Pathophysiology, Pre/postoperative Seizures Predicators and Treatment.

Front Oncol 2022 4;12:905976. Epub 2022 Jul 4.

Department of Neurology, Loma Linda University Medical Center, Loma Linda, CA, United States.

Meningiomas are the most common primary brain tumors accounting for about 30% of all brain tumors. The vast majority of meningiomas are slow-growing and of benign histopathology rendering them curable by surgery alone. Symptomatic lesions depend on the location with signs of mass effect or neurological deficits. Seizures are the presenting symptoms in approximately 30% of cases, which negatively affect quality of life, limit independence, impair cognitive functioning, as well as increase the risk for psychiatric comorbidities including depression. Although surgical resection may offer seizure freedom in 60-90% of meningiomas, seizures persist after surgical resection in approximately 12-19% of patients. Anti-seizure medications (ASMs) are employed in management, however, are limited by adverse neurocognitive side-effects and inefficacy in some patients. The potential predictors of pre- and post-operative seizures in meningioma patients have been identified in the literature. Understanding various factors associated with seizure likelihood in meningioma patients can help guide more effective seizure control and allow for better determination of risk before and after surgery.
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http://dx.doi.org/10.3389/fonc.2022.905976DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9289540PMC
July 2022

Mini-review: Recent advances in post-translational modification site prediction based on deep learning.

Comput Struct Biotechnol J 2022 30;20:3522-3532. Epub 2022 Jun 30.

Department of Computer Science, City University of Hong Kong, Hong Kong Special Administrative Region.

Post-translational modifications (PTMs) are closely linked to numerous diseases, playing a significant role in regulating protein structures, activities, and functions. Therefore, the identification of PTMs is crucial for understanding the mechanisms of cell biology and diseases therapy. Compared to traditional machine learning methods, the deep learning approaches for PTM prediction provide accurate and rapid screening, guiding the downstream wet experiments to leverage the screened information for focused studies. In this paper, we reviewed the recent works in deep learning to identify phosphorylation, acetylation, ubiquitination, and other PTM types. In addition, we summarized PTM databases and discussed future directions with critical insights.
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http://dx.doi.org/10.1016/j.csbj.2022.06.045DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9284371PMC
June 2022

Substantial transition to clean household energy mix in rural China.

Natl Sci Rev 2022 Jul 14;9(7):nwac050. Epub 2022 Mar 14.

College of Urban and Environmental Sciences, Peking University, Beijing 100871, China.

The household energy mix has significant impacts on human health and climate, as it contributes greatly to many health- and climate-relevant air pollutants. Compared to the well-established urban energy statistical system, the rural household energy statistical system is incomplete and is often associated with high biases. Via a nationwide investigation, this study revealed high contributions to energy supply from coal and biomass fuels in the rural household energy sector, while electricity comprised ∼20%. Stacking (the use of multiple sources of energy) is significant, and the average number of energy types was 2.8 per household. Compared to 2012, the consumption of biomass and coals in 2017 decreased by 45% and 12%, respectively, while the gas consumption amount increased by 204%. Increased gas and decreased coal consumptions were mainly in cooking, while decreased biomass was in both cooking (41%) and heating (59%). The time-sharing fraction of electricity and gases (E&G) for daily cooking grew, reaching 69% in 2017, but for space heating, traditional solid fuels were still dominant, with the national average shared fraction of E&G being only 20%. The non-uniform spatial distribution and the non-linear increase in the fraction of E&G indicated challenges to achieving universal access to modern cooking energy by 2030, particularly in less-developed rural and mountainous areas. In some non-typical heating zones, the increased share of E&G for heating was significant and largely driven by income growth, but in typical heating zones, the time-sharing fraction was <5% and was not significantly increased, except in areas with policy intervention. The intervention policy not only led to dramatic increases in the clean energy fraction for heating but also accelerated the clean cooking transition. Higher income, higher education, younger age, less energy/stove stacking and smaller family size positively impacted the clean energy transition.
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http://dx.doi.org/10.1093/nsr/nwac050DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9283105PMC
July 2022

Efficacy of salmeterol and magnesium isoglycyrrhizinate combination treatment in rats with chronic obstructive pulmonary disease.

Sci Rep 2022 Jul 19;12(1):12334. Epub 2022 Jul 19.

Department of Pharmacology and Medicinal Chemistry, Jiangsu Vocational College of Medicine, Yancheng, 224005, Jiangsu, People's Republic of China.

The most classic treatment recommended in the current chronic obstructive pulmonary disease (COPD) guidelines is glucocorticoid and β2 receptor agonist combination, such as salmeterol xinafoate and fluticasone propionate (Sal/Flu), causing many adverse reactions due to hormones. Magnesium isoglycyrrhizinate (MgIG) is an anti-inflammatory glycyrrhizic acid preparation for treating chronic inflammation, contributing to its structure is similar to steroidal anti-inflammatory drugs. In this study, we successfully established COPD rat model by endotracheal-atomized lipopolysaccharide exposure and cigarette smoke induction, as characterized by lung function decline. We discovered that salmeterol xinafoate/MgIG combination could alleviated lung inflammation infiltration, airway wall thickness (AWT) and the secretion of bronchial mucin MUC5AC of COPD rats more than salmeterol xinafoate, MgIG, or salmeterol xinafoate and fluticasone propionate treatment did, as well as reduced inflammatory cells (white blood cells, neutrophils and lymphocytes) accumulation in bronchoalveolar lavage fluid and decreased TNF-α, IL-6 and IL-1β production in the serum of COPD rats. Finally, we found that Moreover, the mechanism involved might be related to the suppression of JAK/STAT signaling pathway. Overall, our studies suggested that MgIG might be a potential alternative adjuvant drug for fluticasone propionate for the clinical treatment of patients with COPD.
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http://dx.doi.org/10.1038/s41598-022-16775-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9296643PMC
July 2022

Parkinson's Disease rs117896735 Variant Regulates INPP5F Expression in Brain Tissues and Increases Risk of Alzheimer's Disease.

J Alzheimers Dis 2022 Jul 13. Epub 2022 Jul 13.

Department of Pathology, The Affiliated Hospital of Weifang Medical University, Weifang, China.

Background: Both INPP5D and INPP5F are members of INPP5 family. INPP5F rs117896735 variant was associated with Parkinson's disease (PD) risk, and INPP5D was an Alzheimer's disease (AD) risk gene. However, it remains unclear about the roles of INPP5F rs117896735 variant in AD.

Objective: We aim to investigate the roles of rs117896735 in AD.

Methods: First, we conducted a candidate variant study to evaluate the association of rs117896735 variant with AD risk using the large-scale AD GWAS dataset. Second, we conducted a gene expression analysis of INPP5F to investigate the expression difference of INPP5F in different human tissues using two large-scale gene expression datasets. Third, we conducted an expression quantitative trait loci analysis to evaluate whether rs117896735 variant regulate the expression of INPP5F. Fourth, we explore the potentially differential expression of INPP5F in AD and control using multiple AD-control gene expression datasets in human brain tissues and whole blood.

Results: We found that 1) rs117896735 A allele was associated with the increased risk of AD with OR = 1.15, 95% CI 1.005-1.315, p = 0.042; 2) rs117896735 A allele could increase INPP5F expression in multiple human tissues; 3) INPP5F showed different expression in different human tissues, especially in brain tissues; 4) INPP5F showed significant expression dysregulation in AD compared with controls in human brain tissues.

Conclusion: Conclusion: We demonstrate that PD rs117896735 variant could regulate INPP5F expression in brain tissues and increase the risk of AD. These finding may provide important information about the role of rs117896735 in AD.
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http://dx.doi.org/10.3233/JAD-220086DOI Listing
July 2022

Association of Maternal Mild Hypothyroidism With Offspring Neurodevelopment in TPOAb-Negative Women: A Prospective Cohort Study.

Front Endocrinol (Lausanne) 2022 29;13:884851. Epub 2022 Jun 29.

State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, China.

Objectives: Adequate maternal thyroid hormone availability is crucial for fetal neurodevelopment, but the role of maternal mild hypothyroidism is not clear. We aim to investigate the association of maternal mild hypothyroidism with neurodevelopment in infants at 1 year of age among TPOAb-negative women.

Methods: The present study was conducted within the Jiangsu Birth Cohort. A total of 793 mother-infant pairs were eligible for the present study. Maternal thyroid function was assessed by measuring serum thyroid-stimulating hormone, free thyroxine, and thyroid peroxidase antibodies. Neurodevelopment of infants was assessed by using the Bayley Scales of Infant and Toddler Development third edition screening test (Bayley-III screening test).

Results: In the multivariate adjusted linear regression analyses, infants of women with subclinical hypothyroidism and isolated hypothyroxinemia were associated with decreased receptive communication scores ( = -0.68, = 0.034) and decreased gross motor scores ( = -0.83, = 0.008), respectively. Moreover, infants of women with high-normal TSH concentrations (3.0-4.0 mIU/L) and low FT4 concentrations were significantly associated with lower gross motor scores ( = -1.19, = 0.032), while no differences were observed in infants when the mothers had a high-normal TSH concentration and normal FT4 levels.

Conclusions: Maternal subclinical hypothyroidism is associated with decreased receptive communication scores in infants at 1 year of age. In addition, maternal TSH concentration greater than 4.0 mIU/L and maternal isolated hypothyroxinemia are associated with impaired gross motor ability of infants, especially in infants of women with high-normal TSH concentrations (3.0-4.0 mIU/L).
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http://dx.doi.org/10.3389/fendo.2022.884851DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9278520PMC
June 2022

Adjusted control rate closely associated with the epidemiologic evolution of the recent COVID-19 wave in Shanghai, with 94.3% of all new cases being asymptomatic on first diagnosis.

Authors:
Lei Huang

J Infect 2022 Jul 16. Epub 2022 Jul 16.

Department of Oncology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China; Medical Center on Aging of Ruijin Hospital, MCARJH, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China. Electronic address:

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http://dx.doi.org/10.1016/j.jinf.2022.07.010DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9288239PMC
July 2022

Direct Synthesis of Biphenyl-2-carbonitriles by Rh(III)-Catalyzed C-H Hiyama Cross-Coupling in Water.

Org Lett 2022 07 13;24(28):5029-5033. Epub 2022 Jul 13.

Department of Chemistry & Chemical Engineering, Gannan Normal University, Ganzhou 341000, China.

This method represents an efficient rhodium(III)-catalyzed -C-H arylation of readily available benzimidate derivatives with diverse arylsilanes in water as a sustainable solvent, enabling the straightforward synthesis of potentially useful biphenyl-2-carbonitrile derivatives. This silicon-based protocol employs benzimidates as both an efficacious directing group and the source of a nitrile group.
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http://dx.doi.org/10.1021/acs.orglett.2c01754DOI Listing
July 2022

Post-Treatment of TiO Film Enables High-Quality SbSe Film Deposition for Solar Cell Applications.

ACS Appl Mater Interfaces 2022 Jul 12. Epub 2022 Jul 12.

CAS Key Laboratory of Materials for Energy Conversion, Department of Materials Science and Engineering, School of Chemistry and Materials Science, University of Science and Technology of China, Hefei 230026, Anhui Province, China.

The TiO thin film is considered as a promising wide band gap electron-transporting material. However, due to the strong Ti-O bond, it displays an inert surface characteristic causing difficulty in the adsorption and deposition of metal chalcogenide films such as SbSe. In this study, a simple CdCl post-treatment is conducted to functionalize the TiO thin film, enabling the induction of nucleation sites and growth of high-quality SbSe. The interfacial treatment optimizes the conduction band offset of TiO/SbSe and leads to an essentially improved TiO/SbSe heterojunction. With this convenient interface functionalization, the power conversion efficiency of the SbSe solar cell is remarkably improved from 2.02 to 6.06%. This study opens up a new avenue for the application of TiO as a wide band gap electron-transporting material in antimony chalcogenide solar cells.
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http://dx.doi.org/10.1021/acsami.2c07157DOI Listing
July 2022

Characteristics of Soil Fungal Communities in Soybean Rotations.

Front Plant Sci 2022 23;13:926731. Epub 2022 Jun 23.

School of Geographical Sciences, Lingnan Normal University, Zhanjiang, China.

Soybean continuous cropping (SC) leads to continuous cropping obstacles, and soil-borne fungal diseases occur frequently. Rotation can alleviate continuous cropping obstacles. However, the long-term effects of continuous cropping and rotation on the structure and function of the fungal community in soil are not clear. In this study, five cropping systems, SC, fallow (CK), fallow-soybean (FS), corn-soybean (CS), and wheat-soybean (WS), were implemented in the long-term continuous cropping area of soybean. After 13 years of planting, high-throughput sequencing was used to evaluate the structure and diversity of soil fungal communities and to study the relationship between fungal communities and soil environmental factors. The results showed that the abundance and diversity of fungal flora in SC soil were the highest. There were significant differences in the formation of soil fungal communities between soybean continuous cropping and the other treatments. There were 355 species of endemic fungi in SC soil. There were 231 and 120 endemic species in WS and CS, respectively. The relative abundance of the potential pathogens , , and in the SC treatment soil was significantly high, and the abundance of all potential pathogens in CK was significantly the lowest. The abundance of and in CS was significantly the lowest. There was a positive correlation between potential pathogens in the soil. The relative abundance of potential pathogens in the soil was significantly positively correlated with the relative abundance of Ascomycetes and negatively correlated with the relative abundance of Basidiomycetes. Potential pathogenic genera had a significant negative correlation with soil OM, available Mn, K and soil pH and a significant positive correlation with the contents of soil available Cu, Fe, and Zn. In general, the fungal communities of SC, FS, WS, and CS were divided into one group, which was significantly different from CK. WS and CS were more similar in fungal community structure. The CK and CS treatments reduced the relative abundance of soil fungi and potential pathogens. Our study shows that SC and FS lead to selective stress on fungi and pathogenic fungi and lead to the development of fungal community abundance and diversity, while CK and CS can reduce this development, which is conducive to plant health.
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http://dx.doi.org/10.3389/fpls.2022.926731DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9260669PMC
June 2022
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