Publications by authors named "Lei Guo"

1,318 Publications

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Insight into the anti-corrosion performance of two food flavors as eco-friendly and ultra-high performance inhibitors for copper in sulfuric acid medium.

J Colloid Interface Sci 2021 Nov 18. Epub 2021 Nov 18.

School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400044, China. Electronic address:

2,5-dihydroxy-1,4-dithiane (DDD) and 2,5-dimethy- [1.4] dithiane-2,5-diol (DTDD) two food flavors as environmentally-friendly inhibitors for Cu in 0.5 mol/L HSO media were researched via theoretical calculation and experimental ways. Electrochemical measurement data showed that DDD and DTDD can exhibit high level anti-corrosion feature. The anti-corrosion efficiency of DDD and DTDD were as high as 99.6% and 98.9%, respectively. The atomic force microscope (AFM) and scanning electron microscope (SEM) tests showed that the Cu specimens were immersed in the HSO with 5 mM DDD and DTDD for 30 h at the 298 K, and the Cu specimen surface was still smooth. Besides, the adsorption of DDD and DTDD at the interface of Cu/solution was comply with Langmuir adsorption. Theoretical calculation data showed that DDD exhibit more ascendant anti-corrosion feature than DTDD.
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http://dx.doi.org/10.1016/j.jcis.2021.11.085DOI Listing
November 2021

Ending tuberculosis in China: health system challenges.

Lancet Public Health 2021 Dec;6(12):e948-e953

Global Health Research Center, Duke Kunshan University, Jiangsu, China; Duke Global Health Institute, Duke University, Durham, NC, USA.

China has made remarkable progress in reducing tuberculosis cases and deaths during the past three decades; however, it is still far from achieving the targets set out in the WHO End TB Strategy. Since the 2000s, China has tried to transform its vertical tuberculosis control programme led by the Chinese Center for Disease Control and Prevention (CDC) into an integrated system under the collaboration of CDC, tuberculosis-designated hospitals, and primary health centres. Such a transition has faced many challenges. Profit-driven practices in hospitals designated to tuberculosis and an absence of adequate tuberculosis-related training for health professionals are partially jeopardising the quality of tuberculosis care. In addition, primary health-care providers are not incentivised to make referrals and manage cases effectively. The CDC does not have the administrative power to influence hospital practices or deploy resources to support community-based tuberculosis control activities. Furthermore, an absence of policy coherence and effective coordination causes challenges for quality tuberculosis care that is affordable and accessible. Improving policy dialogues and multi-level coordination within the government is fundamental to successfully ending tuberculosis in China and other countries facing similar challenges.
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http://dx.doi.org/10.1016/S2468-2667(21)00203-6DOI Listing
December 2021

Interaction of B-type starch with gluten skeleton improves wheat dough mixing properties by stabilizing gluten micro-structure.

Food Chem 2022 Mar 13;371:131390. Epub 2021 Oct 13.

State Key Laboratory of Crop Stress Biology in Arid Areas and College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, China. Electronic address:

Some recent studies have revealed individual and the combined interactions of gluten and starch affecting dough mixing properties. However, the combined influence of high-molecular-weight glutenin subunits (HMW-GS) and starch on dough mixing and rheological properties requires elucidation. Thus four recombinant inbred lines, SS 1, SS 2, ZZ 1 and ZZ 2, were selected based on their HMW-GSs compositions. Compared to ZZ 1 and ZZ 2, both SS 1 and SS 2 carried superior HMW-GS alleles, and exhibited extended dough development and stability time, indicating their significant dough mixing characteristics. The gluten skeleton of the wheat lines SS 2 and ZZ 2 with higher B-type starch proportions exhibited fewer breakages along with the rise of dough temperature during mixing. Higher content of B-type starch strengthens interaction between starch and gluten skeleton at the dough heating stage, suggesting a specific range of B-type starch proportion can improve dough mixing characteristics.
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http://dx.doi.org/10.1016/j.foodchem.2021.131390DOI Listing
March 2022

Evaluation of an EZH2 inhibitor in patient-derived orthotopic xenograft models of pediatric brain tumors alone and in combination with chemo- and radiation therapies.

Lab Invest 2021 Nov 20. Epub 2021 Nov 20.

Texas Children's Cancer Center, Texas Children's Hospital, Baylor College of Medicine, Houston, TX, USA.

Brain tumors are the leading cause of cancer-related death in children. Tazemetostat is an FDA-approved enhancer of zeste homolog (EZH2) inhibitor. To determine its role in difficult-to-treat pediatric brain tumors, we examined EZH2 levels in a panel of 22 PDOX models and confirmed EZH2 mRNA over-expression in 9 GBM (34.6 ± 12.7-fold) and 11 medulloblastoma models (6.2 ± 1.7 in group 3, 6.0 ± 2.4 in group 4) accompanied by elevated H3K27me3 expression. Therapeutic efficacy was evaluated in 4 models (1 GBM, 2 medulloblastomas and 1 ATRT) via systematically administered tazemetostat (250 and 400 mg/kg, gavaged, twice daily) alone and in combination with cisplatin (5 mg/kg, i.p., twice) and/or radiation (2 Gy/day × 5 days). Compared with the untreated controls, tazemetostat significantly (P < 0.05) prolonged survival times in IC-L1115ATRT (101% at 400 mg/kg) and IC-2305GBM (32% at 250 mg/kg, 45% at 400 mg/kg) in a dose-dependent manner. The addition of tazemetostat with radiation was evaluated in 3 models, with only one [IC-1078MB (group 4)] showing a substantial, though not statistically significant, prolongation in survival compared to radiation treatment alone. Combining tazemetostat (250 mg/kg) with cisplatin was not superior to cisplatin alone in any model. Analysis of in vivo drug resistance detected predominance of EZH2-negative cells in the remnant PDOX tumors accompanied by decreased H3K27me2 and H3K27me3 expressions. These data supported the use of tazemetostat in a subset of pediatric brain tumors and suggests that EZH2-negative tumor cells may have caused therapy resistance and should be prioritized for the search of new therapeutic targets.
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http://dx.doi.org/10.1038/s41374-021-00700-8DOI Listing
November 2021

The Use of Imaging Equipment During Cosmetic Laser Surgery to Reduce Injury to the Surgeon's Eyes.

Aesthetic Plast Surg 2021 Nov 17. Epub 2021 Nov 17.

Department of Plastic Surgery, Affiliated Hospital of Jiujiang University, No. 57 Xun Yang East Road, Jiujiang, 332000, Jiangxi, China.

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http://dx.doi.org/10.1007/s00266-021-02667-9DOI Listing
November 2021

Epigenetic glycosylation of SARS-CoV-2 impact viral infection through DC&L-SIGN receptors.

iScience 2021 Dec 11;24(12):103426. Epub 2021 Nov 11.

Institute of Medical Biology, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, China.

Glycosylation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike glycoprotein mediates viral entry and immune escape. While glycan site is determined by viral genetic code, glycosylation is completely dependent on host cell post-translational modification. Here, by producing SARS-CoV-2 virions from various host cell lines, viruses of different origins with diverse spike protein glycan patterns were revealed. Binding affinities to C-type lectin receptors (CLRs) DC&L-SIGN differed in the different glycan pattern virions. Although none of the CLRs supported viral productive infection, viral &cis-infection mediated by the CLRs were substantially changed among the different virions. Specifically, &cis-infection of virions with a high-mannose structure (ManGlcNAc) at the N1098 glycan site of the spike postfusion trimer were markedly enhanced. Considering L-SIGN co-expression with ACE2 on respiratory tract cells, our work underlines viral epigenetic glycosylation in authentic viral infection and highlights the attachment co-receptor role of DC&L-SIGN in SARS-CoV-2 infection and prevention.
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http://dx.doi.org/10.1016/j.isci.2021.103426DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8582233PMC
December 2021

Metabolism of a selective serotonin and norepinephrine reuptake inhibitor duloxetine in liver microsomes and mice.

Drug Metab Dispos 2021 Nov 16. Epub 2021 Nov 16.

Department of Pathology, Baylor College of Medicine, United States

Duloxetine (DLX) is a dual serotonin and norepinephrine reuptake inhibitor, widely used for the treatment of major depressive disorder. Although DLX has shown good efficacy and safety, serious adverse effects (e.g., liver injury) have been reported. The mechanisms associated with DLX-induced toxicity remain elusive. Drug metabolism plays critical roles in drug safety and efficacy. However, the metabolic profile of DLX in mice is not available although mice serve as commonly used animal models for mechanistic studies of drug-induced adverse effects. Our study revealed 39 DLX metabolites in human/mouse liver microsomes and mice. Of note, thirteen metabolites are novel, including five -acetyl cysteine adducts and one GSH adduct associated with DLX. Additionally, the species differences of certain metabolites were observed between human and mouse liver microsomes. CYP1A2 and CYP2D6 are primary enzymes responsible for the formation of DLX metabolites in liver microsomes, including DLX-GSH adducts. In summary, a total of 39 DLX metabolites were identified and specie differences were noticed The roles of CYP450s in DLX metabolite formation were also verified using human recombinant P450 enzymes and corresponding chemical inhibitors. Further studies are warranted to address the exact role of DLX metabolism in its adverse effects (, human primary hepatocytes) and ( Cyp1a2-null mice). Current study systematically investigated DLX metabolism and bioactivation in liver microsomes and mice. This study provided a global view of DLX metabolism and bioactivation in liver microsomes and mice, which are very valuable to further elucidate the mechanistic study of DLX-related adverse effects and drug-drug interaction from metabolic aspects.
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http://dx.doi.org/10.1124/dmd.121.000633DOI Listing
November 2021

Case Report: Preliminary Images From an Electromagnetic Portable Brain Scanner for Diagnosis and Monitoring of Acute Stroke.

Front Neurol 2021 29;12:765412. Epub 2021 Oct 29.

School of Information Technology and Electrical Engineering, University of Queensland, Brisbane, QLD, Australia.

Electromagnetic imaging is an emerging technology which promises to provide a mobile, and rapid neuroimaging modality for pre-hospital and bedside evaluation of stroke patients based on the dielectric properties of the tissue. It is now possible due to technological advancements in materials, antennae design and manufacture, rapid portable computing power and network analyses and development of processing algorithms for image reconstruction. The purpose of this report is to introduce images from a novel, portable electromagnetic scanner being trialed for bedside and mobile imaging of ischaemic and haemorrhagic stroke. A prospective convenience study enrolled patients (January 2020 to August 2020) with known stroke to have brain electromagnetic imaging, in addition to usual imaging and medical care. The images are obtained by processing signals from encircling transceiver antennae which emit and detect low energy signals in the microwave frequency spectrum between 0.5 and 2.0 GHz. The purpose of the study was to refine the imaging algorithms. Examples are presented of haemorrhagic and ischaemic stroke and comparison is made with CT, perfusion and MRI T2 FAIR sequence images. Due to speed of imaging, size and mobility of the device and negligible environmental risks, development of electromagnetic scanning scanner provides a promising additional modality for mobile and bedside neuroimaging.
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http://dx.doi.org/10.3389/fneur.2021.765412DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8589013PMC
October 2021

Insights into the newly synthesized N-doped carbon dots for Q235 steel corrosion retardation in acidizing media: A detailed multidimensional study.

J Colloid Interface Sci 2021 Oct 30;608(Pt 2):2039-2049. Epub 2021 Oct 30.

Faculty of Natural Sciences, Karshi State University, Karshi 180100, Uzbekistan.

N-doped carbon quantum dots (NCQDs) were synthesized by a hydrothermal method using folic acid and o-phenylenediamine as precursors. The inhibition behaviour of the NCQDs on Q235 steel in 1 M HCl solution was appraised through electrochemical impedance spectroscopy (EIS), potentiodynamic polarization curves (PDP), and surface analysis. The results demonstrated that the synthesized NCQDs had an effective anticorrosion effect on Q235 steel, and the corrosion inhibition efficiency of 150 mg/L NCQDs reached 95.4%. Additionally, the analysis of the PDP corrosion potential changes indicated that the NCQDs acted as a mixed corrosion inhibitor. Moreover, the NCQDs adsorbed onto the surface of steel by coordinating its electron-rich atoms with the iron metal to form a protective film, which slowed the dissolution reaction of the anodic metal to achieve corrosion inhibition. The adsorption mechanism of the NCQDs was consistent with Langmuir adsorption, including physical and chemical adsorption. Therefore, this work can inspire and facilitate, to a certain extent, the future application of doped carbon quantum dots as efficient corrosion inhibitors in pickling solutions.
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http://dx.doi.org/10.1016/j.jcis.2021.10.160DOI Listing
October 2021

Gut microbes enlarged the protective effect of transplanted regulatory B cells on rejection of cardiac allografts.

J Heart Lung Transplant 2021 Dec 25;40(12):1502-1516. Epub 2021 Aug 25.

Department of Cardiothoracic Surgery, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China. Electronic address:

Background: Regulatory B cells (Bregs) play an important role in maintaining immune homeostasis and have the potential to induce tolerance. Previous work has found that Breg cells are involved in heart transplantation tolerance. However, the effect of Breg on the transplantation tolerance and the underlying mechanisms remain to be clarified.

Methods: Using a within-species heart transplantation model, we aimed to investigate the role of CD19CD5CD1d Bregs isolated from transplanted mice in preventing transplant rejection in vivo. We also explored the effects of CD40 and tumor necrosis factor receptor-associated factor 6 (TRAF6) ubiquitin ligase on Breg-mediated prolongation of survival in heart transplant (HT) mice, and the regulatory effects of downstream Cdk4 and Cdk6 proteins on dendritic cells (DCs), which clarified the function and molecular mechanism of Breg cells in HT mice.

Results: Our data suggest that adoptive transfer of the transplanted Bregs served as an effective tolerance-inducing mechanism in HT mice and was involved in the CD40-TRAF6 signaling pathway in DCs. Moreover, DCs collected from the Breg treated HT mice also prolonged the survival of HT mice. Furthermore, DC-specific knockout of TRAF6 diminished Breg-mediated prolongation of survival in HT mice. Interestingly, gut microbes from donors increased the survival of cardiac allografts both in both the absence and presence of Bregs but were not implicated in CD40-TRAF6 signaling.

Conclusions: These findings reveal a role of Breg cells in the induction of transplantation tolerance through the blockade of the CD40-TRAF6 signaling pathway, which might be used in the treatment of HT in the clinic.
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http://dx.doi.org/10.1016/j.healun.2021.08.008DOI Listing
December 2021

γ-Glutamyl transpeptidase-activated indole-quinolinium based cyanine as a fluorescence turn-on nucleolus-targeting probe for cancer cell detection and inhibition.

Talanta 2022 Jan 28;237:122898. Epub 2021 Sep 28.

School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou, 510006, China. Electronic address:

A nucleolus as a prominent sub-nuclear, membraneless organelle plays a crucial role in ribosome biogenesis, which is in the major metabolic demand in a proliferating cell, especially in aggressive malignancies. We develop a γ-glutamyltranspeptidase (GGT)-activatable indole-quinolinium (QI) based cyanine consisting of a novel tripeptide fragment (Pro-Gly-Glu), namely QI-PG-Glu as a turn-on red fluorescent probe for the rapid detection of GGT-overexpressed A549 cancer cells in vivo. QI-PG-Glu can be triggered by GGT to rapidly release an activated fluorophore, namely HQI, in two steps including the cleavage of the γ-glutamyl group recognized by GGT and the rapid self-driven cyclization of the Pro-Gly linker. HQI exhibits dramatically red fluorescence upon binding to rRNA for imaging of nucleolus in live A549 cells. HQI also intervenes in rRNA biogenesis by declining the RNA Polymerase I transcription, thus resulting in cell apoptosis via a p53 dependent signaling pathway. Our findings may provide an alternative avenue to develop multifunctional cancer cell-specific nucleolus-targeting fluorescent probes with potential anti-cancer effects.
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http://dx.doi.org/10.1016/j.talanta.2021.122898DOI Listing
January 2022

YAP1/MMP7/CXCL16 axis affects efficacy of neoadjuvant chemotherapy via tumor environment immunosuppression in triple-negative breast cancer.

Gland Surg 2021 Sep;10(9):2799-2814

Clinical Research Center for Breast Cancer Control and Prevention in Hunan Province, Department of General Surgery, Xiangya Hospital, Central South University, Changsha, China.

Background: To evaluate the association of potential YAP1/MMP7/CXCL16 axis and tumor infiltrating lymphocytes (TILs) related chemo-response in triple-negative breast cancer (TNBC) patients.

Methods: We estimated the messenger RNA (mRNA) expression levels of Yes-associated protein 1 (YAP1), MMP7, and CXCL16 in paired TNBC tumor/para-tumor tissues by quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR), and performed statistical analysis according to neoadjuvant chemotherapy (NAC) response. Based on The Cancer Genome Atlas (TCGA) data, we noticed outstanding expression of MMP7/CXCL16 in TNBC cases, as well as associations between MMP7/CXCL16 and HIPPO-YAP1-relevant kinases. We also performed gene set enrichment analysis (GSEA) between MMP7/CXCL16 and YAP1-associated pathways. Western blotting assay was employed to evaluate YAP1/MMP7/CXCL16 expression and their modulation sequence. Logistic model stepwise regression analysis was used to assess YAP1, MMP7, CXCL16, and TILs as therapeutic predictors. Residual cancer burden (RCB) score was calculated and statistically analyzed according to intensity of these variables, and receiver operating characteristic (ROC) curve also showed their predictive value in NAC response. Recruitment efficacy for CD4+/CD8+ TIL cells (TCGA data) as well as quantified TIL cells density were both explored according to YAP1, MMP7, and CXCL16 expression level.

Results: Up-regulation of YAP1/MMP7 and down-regulation of CXCL16 were both significant in TNBC cases with poor NAC response. Inhibition of YAP1 induced down-regulation of MMP7 and up-regulation of CXCL16, whereas inhibition of MMP7 also induced up-regulation of CXCL16. It was also shown that MMP7/CXCL16 was enriched in the YAP1-related pathway. Activation of the YAP1/MMP7/CXCL16 axis obviously affected RCB of TNBC cases. The ROC curve also supported the predictive value of YAP1/MMP7/CXCL16 axis and TILs density in NAC response prospect. The density of TILs, meanwhile, demonstrated a strong link with the YAP1/MMP7/CXCL16 axis. Over expression of YAP1/MMP7 significantly suppressed recruitment of CD4+/CD8+ TILs, while CXCL16 over expression had a beneficial impact on anti-tumor immune.

Conclusions: Over expression of causes up-regulation of MMP7 and down-regulation of CXCL16, which suppressed CD4+/CD8+ TILs recruitment and indirectly affected NAC response of TNBC patients.
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http://dx.doi.org/10.21037/gs-21-612DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8514296PMC
September 2021

Evaluation of the effectiveness and safety of cupping therapy in the treatment of asthma: A protocol for systematic review and meta-analysis.

Medicine (Baltimore) 2021 Oct;100(41):e27518

Changchun University of Chinese Medicine, 1035 Boshuo Road, Changchun City, Jilin Province, China.

Background: Asthma is one of the most common chronic airway diseases and is characterized by wheezing, dyspnea, chest tightness, and coughing. These symptoms reduce the patient's quality of life and limit physical activity in daily life. However, there is no systematic review of the efficacy of cupping therapy in the treatment of asthma. To evaluate the efficacy and safety of cupping in the treatment of asthma, we conducted a systematic review and meta-analysis of published randomized clinical trials of cupping in the treatment of asthma.

Methods: We will search the following Chinese and English databases: China National Knowledge Infrastructure, China Science and Periodical Database, Wanfang Database, China Biomedical Literature Database, PubMed, Embase, Cochrane Library. All of the above electronic databases will be searched from inception to August 22, 2021. In addition, we will manually search for conference papers, ongoing experiments, and internal reports to supplement the studies retrieved via electronic search. We will use the Review Manager 5.4 provided by Cochrane Collaboration Network for statistical analysis.

Results: The study will prove the effectiveness and safety of cupping in the treatment of asthma.

Conclusion: We plan to submit this systematic review to a peer-reviewed journal.

Inplasy Registration Number: INPLASY202180104.
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http://dx.doi.org/10.1097/MD.0000000000027518DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8519211PMC
October 2021

Chromosomal variants accumulate in genomes of the spontaneous aborted fetuses revealed by chromosomal microarray analysis.

PLoS One 2021 2;16(11):e0259518. Epub 2021 Nov 2.

Guangdong-Hong Kong Metabolism & Reproduction Joint Laboratory, Guangdong Second Provincial General Hospital, Guangzhou, China.

Spontaneous abortion is an impeding factor for the success rates of human assistant reproductive technology (ART). Causes of spontaneous abortion include not only the pregnant mothers' health conditions and lifestyle habits, but also the fetal development potential. Evidences had shown that fetal chromosome aneuploidy is associated with fetal spontaneous abortion, however, it is still not definite that whether other genome variants, like copy number variations (CNVs) or loss of heterozygosity (LOHs) is associated with the spontaneous abortion. To assess the relationship between the fetal genome variants and abortion during ART, a chromosomal microarray data including chromosomal information of 184 spontaneous aborted fetuses, 147 adult female patients and 78 adult male patients during ART were collected. We firstly analyzed the relationship of fetal aneuploidy with maternal ages and then compared the numbers and lengths of CNVs (< 4Mbp) and LOHs among adults and aborted fetuses. In addition to the already known association between chromosomal aneuploidy and maternal ages, from the chromosomal microarray data we found that the numbers and the accumulated lengths of short CNVs and LOHs in the aborted fetuses were significantly larger or longer than those in adults. Our findings indicated that the increased numbers and accumulated lengths of CNVs or LOHs might be associated with the spontaneous abortion during ART.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0259518PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8562782PMC
November 2021

[A review of brain-like spiking neural network and its neuromorphic chip research].

Sheng Wu Yi Xue Gong Cheng Xue Za Zhi 2021 Oct;38(5):986-994

State Key Laboratory of Reliability and Intelligence of Electrical Equipment, Hebei University of Technology, Tianjin 300130, P.R.China.

Under the current situation of the rapid development of brain-like artificial intelligence and the increasingly complex electromagnetic environment, the most bionic and anti-interference spiking neural network has shown great potential in computing speed, real-time information processing, and spatiotemporal data processing. Spiking neural network is the core part of brain-like artificial intelligence, which realizes brain-like computing by simulating the structure of biological neural network and the way of information transmission. This article first summarizes the advantages and disadvantages of the five models, and analyzes the characteristics of several network topologies. Then, it summarizes the spiking neural network algorithms. The unsupervised learning based on spike timing dependent plasticity (STDP) rules and four types of supervised learning algorithms are analyzed. Finally, the research on brain-like neuromorphic chips at home and abroad are reviewed. This paper aims to provide learning ideas and research directions for new colleagues in the field of spiking neural network.
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http://dx.doi.org/10.7507/1001-5515.202011005DOI Listing
October 2021

Up-regulation of SIRT1 induced by 17beta-estradiol promotes autophagy and inhibits apoptosis in osteoblasts.

Aging (Albany NY) 2021 10 28;13(20):23652-23671. Epub 2021 Oct 28.

Department of Orthopedics, The First Hospital of China Medical University, Shenyang 110001, Liaoning, China.

Osteoporosis is a common systemic skeletal metabolism disorder resulting in bone fragility and increased fracture risk. Silent information regulator factor 2 homolog 1 (SIRT1) is crucial in the regulation of several biological processes, including bone metabolism, autophagy, apoptosis, and aging. This study aimed to assess whether the up-regulation of SIRT1 induced by 17beta-estradiol (17β-E2) could promote autophagy and inhibit apoptosis in osteoblasts via the AMPK-mTOR and FOXO3a pathways, respectively. The study found that 17β-E2 (10 M) administration induced the up-regulation of SIRT1 in osteoblasts. Up-regulation of SIRT1 induced by 17β-E2 increased the expression level of LC3, Beclin-1, Bcl-2, p-AMPK, FOXO3a but decreased caspase-3 and p-mTOR expression, and then promoted autophagy and inhibited apoptosis. More autophagosomes were observed under a transmission electron microscope (TEM) in 17β-E2 and SRT1720 (a selective SIRT1 activator) co-treated group. When Ex527 (a SIRT1-specific inhibitor) was pretreated, the reversed changes were observed. Taken together, our findings demonstrated that the up-regulation of SIRT1 induced by 17β-E2 could promote autophagy via the AMPK-mTOR pathway and inhibit apoptosis via the FOXO3a activation in osteoblasts, and SIRT1 might become a more significant target in osteoporosis treatment.
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http://dx.doi.org/10.18632/aging.203639DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8580331PMC
October 2021

Increased Expression of Integrin Alpha 6 in Nucleus Pulposus Cells in Response to High Oxygen Tension Protects against Intervertebral Disc Degeneration.

Oxid Med Cell Longev 2021 18;2021:8632823. Epub 2021 Oct 18.

Department of Orthopedics, Changzheng Hospital, The Naval Medical University, Shanghai, China.

The destruction of the low oxygen microenvironment in nucleus pulposus (NP) cells played a critical role in the pathogenesis of intervertebral disc degeneration (IVDD). The purpose of this study was to determine the potential role of integrin alpha 6 (ITG 6) in NP cells in response to high oxygen tension (HOT) in IVDD. Immunofluorescence staining and western blot analysis showed that the levels of ITG 6 expression were increased in the NP tissue from IVDD patients and the IVDD rat model with mild degeneration, which were reduced as the degree of degeneration increases in severity. In NP cells, the treatment of HOT resulted in upregulation of ITG 6 expression, which could be alleviated by blocking the PI3K/AKT signaling pathway. Further studies found that ITG 6 could protect NP cells against HOT-induced apoptosis and oxidative stress and protect NP cells from HOT-inhibited ECM protein synthesis. Upregulation of ITG 6 expression by HOT contributed to maintaining NP tissue homeostasis through the interaction with hypoxia-inducible factor-1 (HIF-1). Furthermore, silencing of ITG 6 could obviously accelerate puncture-induced IVDD. Taken together, these results revealed that the increase of ITG 6 expression by HOT in NP cells might be a protective factor in IVD degeneration as well as restore NP cell function.
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http://dx.doi.org/10.1155/2021/8632823DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8545551PMC
October 2021

Predictors of Adverse Events Among Chronic Total Occlusion Patients Undergoing Successful Percutaneous Coronary Intervention and Medical Therapy.

Clin Interv Aging 2021 14;16:1847-1855. Epub 2021 Oct 14.

Department of Cardiology, The First Affiliated Hospital of Dalian Medical University, Dalian City, People's Republic of China.

Objective: Limited data are available on the predictors of major adverse cardiac events (MACE) after a successful coronary chronic total occlusion (CTO) percutaneous coronary intervention (PCI) and medical therapy. This study aimed to identify predictors of MACE in CTO patients undergoing successful recanalization and medical therapy.

Methods: A total of 2015 patients with CTOs were enrolled. About 718 patients underwent successful CTO recanalization, and 1297 patients received medical therapy. The primary outcome was the frequency of MACE, defined as a composite of cardiac death, myocardial infarction, and target-vessel revascularization. Multivariate models were used to determine predictors of MACE.

Results: In successful CTO recanalization group, MACE occurred in 123 (17.1%) patients. In multivariate analysis, heart failure (hazard ratio [HR] 1.77, 95% confidence interval [CI]: 1.04-3.04, = 0.036) was identified as independent predictors for MACE in successful CTO recanalization. Additionally, in medical therapy group, the significant predictors of MACE were male gender (HR 1.53, 95% CI: 1.13-2.05, = 0.005), diabetes mellitus (HR 1.39, 95% CI: 1.11-1.74, = 0.003), heart failure (HR 1.44, 95% CI: 1.10-1.87, = 0.007), J-CTO score (HR 1.17, 95% CI: 1.07-1.28, = 0.001) and multivessel disease (HR 2.20, 95% CI: 1.42-3.39, < 0.001).

Conclusion: Heart failure was predictor for composite cardiovascular events in patients with CTO after successful recanalization. Male gender, diabetes mellitus, heart failure, J-CTO score and multivessel disease were predictors of MACE in CTO patients with medical therapy.
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http://dx.doi.org/10.2147/CIA.S337069DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8523319PMC
November 2021

Efficient and portable cellulose-based colorimetric test paper for metal ion detection.

Carbohydr Polym 2021 Nov 6;274:118635. Epub 2021 Sep 6.

State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510641, China. Electronic address:

Fabrication of metal ion detection materials generally involved problems such as high cost and complicated processes of pretreatment and operation. Herein, a novel colorimetric test paper for metal ions detection was developed based on functionalized cellulose fibers. Acetoacetyl groups were introduced on cellulose fibers by a surface esterification process. The obtained cellulose acetoacetate (CAA) fibers were made into CAA paper via a paper-making process. The CAA paper possessed robust mechanical property, thermal stability selectivity and rapid response to Fe and Cu ions, with an obvious naked-eye color change within 5 s. The mechanism of this visual recognition for metal ions due to that the acetoacetyl groups coordination chelated with metal ion to form six-membered ring structure, further leading to the color change of the materials. It provided a facile and universal method to prepare efficient and portable cellulose-based test paper, which has great potential in metal ion detection field.
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http://dx.doi.org/10.1016/j.carbpol.2021.118635DOI Listing
November 2021

Adaptive Clustering Distorted Born Iterative Method for Microwave Brain Tomography with Stroke Detection and Classification.

IEEE Trans Biomed Eng 2021 Oct 22;PP. Epub 2021 Oct 22.

A modified distorted Born iterative method (DBIM), which includes clustering of reconstructed electrical properties (EPs) after certain iterations, is presented for brain imaging aiming at stroke detection and classification. For this approach to work, a rough estimation of number of different materials (or bio-tissues) in the imaged domain and their corresponding rough dielectric properties (permittivity and conductivity) are needed as a prior information. The proposed adaptive clustering DBIM (AC-DBIM) is compared with three conventional methods (DBIM, multiplicative regularized contrast source inversion (MR-CSI), and CSI for shape and location reconstruction (SL-CSI)) in two-dimensional scenario on a head phantom and numerical head model with different strokes. Three-dimensional simulations are also conducted to indicate the suitability of AC-DBIM in real-life brain imaging. Lastly, the proposed algorithm is assessed using a clinical electromagnetic head scanner developed on phantoms. The simulation and experimental results show superiority of AC-DBIM compared to conventional methods. AC-DBIM achieves significant improvement in the size and shape reconstruction and reduction in errors and standard deviation of the reconstructed _r and at clinical scenarios compared with conventional DBIM.
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http://dx.doi.org/10.1109/TBME.2021.3122113DOI Listing
October 2021

Immune-Related LncRNAs Affect the Prognosis of Osteosarcoma, Which Are Related to the Tumor Immune Microenvironment.

Front Cell Dev Biol 2021 7;9:731311. Epub 2021 Oct 7.

Musculoskeletal Tumor Center, Peking University People's Hospital, Beijing, China.

Abnormal expression of lncRNA is closely related to the occurrence and metastasis of osteosarcoma. The tumor immune microenvironment (TIM) is considered to be an important factor affecting the prognosis and treatment of osteosarcoma. This study aims to explore the effect of immune-related lncRNAs (IRLs) on the prognosis of osteosarcoma and its relationship with the TIM. Ninety-five osteosarcoma samples from the TARGET database were included. Iterative LASSO regression and multivariate Cox regression analysis were used to screen the IRLs signature with the optimal AUC. The predict function was used to calculate the risk score and divide osteosarcoma into a high-risk group and low-risk group based on the optimal cut-off value of the risk score. The lncRNAs in IRLs signature that affect metastasis were screened for validation. Single sample gene set enrichment analysis (ssGSEA) and ESTIMATE algorithms were used to evaluate the role of TIM in the influence of IRLs on osteosarcoma prognosis. Ten IRLs constituted the IRLs signature, with an AUC of 0.96. The recurrence and metastasis rates of osteosarcoma in the high-risk group were higher than those in the low-risk group. experiments showed that knockdown of lncRNA (AC006033.2) could increase the proliferation, migration, and invasion of osteosarcoma. ssGSEA and ESTIMATE results showed that the immune cell content and immune score in the low-risk group were generally higher than those in the high-risk group. In addition, the expression levels of immune escape-related genes were higher in the high-risk group. The IRLs signature is a reliable biomarker for the prognosis of osteosarcoma, and they alter the prognosis of osteosarcoma. In addition, IRLs signature and patient prognosis may be related to TIM in osteosarcoma. The higher the content of immune cells in the TIM of osteosarcoma, the lower the risk score of patients and the better the prognosis. The higher the expression of immune escape-related genes, the lower the risk score of patients and the better the prognosis.
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http://dx.doi.org/10.3389/fcell.2021.731311DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8529014PMC
October 2021

Ultrasensitive and Self-Powered Terahertz Detection Driven by Nodal-Line Dirac Fermions and Van der Waals Architecture.

Adv Sci (Weinh) 2021 Oct 19:e2102088. Epub 2021 Oct 19.

State Key Laboratory for Infrared Physics, Shanghai Institute of Technical Physics, Chinese Academy of Sciences, 500 Yu-tian Road, Shanghai, 200083, China.

Terahertz detection has been highly sought to open a range of cutting-edge applications in biomedical, high-speed communications, astronomy, security screening, and military surveillance. Nonetheless, these ideal prospects are hindered by the difficulties in photodetection featuring self-powered operation at room temperature. Here, this challenge is addressed for the first time by synthesizing the high-quality ZrGeSe with extraordinary quantum properties of Dirac nodal-line semimetal. Benefiting from its high mobility and gapless nature, a metal-ZrGeSe-metal photodetector with broken mirror symmetry allows for a high-efficiency photoelectric conversion assisted by the photo-thermoelectric effect. The designed architecture features ultrahigh sensitivity, excellent ambient stability, and an efficient rectified signal even above 0.26 THz. Maximum responsivity larger than 0.11 A W , response time of 8.3 µs, noise equivalent power (NEP) less than 0.15 nW Hz , and demonstrative imaging application are all achieved. The superb performances with a lower dark current and NEP less than 15 pW Hz are validated through integrating the van der Waals heterostructure. These results open up an appealing perspective to explore the nontrivial topology of Dirac nodal-line semimetal by devising the peculiar device geometry that allows for a novel roadmap to address targeted terahertz application requirements.
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http://dx.doi.org/10.1002/advs.202102088DOI Listing
October 2021

Asymmetric constellation transmission for a coherent free-space optical system with spatial diversity.

Opt Lett 2021 Oct;46(20):5157-5160

Spatial diversity is widely recognized as an effective technique for free-space optical (FSO) communication to combat channel fading. Most existing works on spatial diversity in FSO systems focus on intensity modulation and direct detection, while fewer mention coherent optical detection, which excels in optical receiver responsivity. Spatial diversity enables the FSO coherent system to demodulate the signal components of in-phase () and quadrature () separately and thus simplify the coherent detector. More importantly, due to the differential channel conditions between and components, we are offered a chance to realize an asymmetric quadrature amplitude modulation (QAM) constellation that can utilize the channel capacity of both and components more sufficiently. Performance results demonstrate that the simplified structure and improved spectrum efficiency can be realized in the asymmetric QAM transmission system with an acceptable bit-error-rate loss.
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http://dx.doi.org/10.1364/OL.439314DOI Listing
October 2021

Channel prediction for intelligent FSO transmission system.

Opt Express 2021 Aug;29(17):27882-27899

Free space optical (FSO) communication has attracted significant attention due to its high transmission rate and information security. Nevertheless, the FSO link is sensitive to various weather conditions which limit its application range. Thus, research on the FSO channel plays an important role for combatting channel fading. In the paper, we first establish a FSO transmission testbed to obtain received signal strength indication (RSSI) information of optical signal. Then we utilize an environmental chamber for indoor experiments to simulate weather changes in the real world. Finally, based on the true meteorological dataset from the official department and the RSSI dataset from the experiments, we employ expanded gated recurrent unit (GRU) neural networks to implement the FSO channel prediction. The results demonstrate that the proposed scheme can achieve the prediction of FSO channel fading with a high precision, where the absolute percentage error (APE) values lower than 6.9% account for up to ninety% of results.
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http://dx.doi.org/10.1364/OE.433493DOI Listing
August 2021

Metastatic NSCLCs With Limited Tissues: How to Effectively Identify Driver Alterations to Guide Targeted Therapy in Chinese Patients.

JTO Clin Res Rep 2021 May 24;2(5):100167. Epub 2021 Mar 24.

Department of Pathology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People's Republic of China.

Introduction: Molecular diagnostics of newly diagnosed patients with metastatic NSCLC (mNSCLC) with limited tissue samples often face several obstacles in routine practice using next-generation sequencing (NGS), mainly owing to insufficient tissue or DNA; thus, how to effectively identify the molecular profiling of these cases to accurately guide targeted therapy remains elusive. We evaluated whether an optimized workflow with the combined use of multiple technologies could be helpful.

Methods: Tissue NGS was used as the frontline method. Amplification refractory mutation system polymerase chain reaction, immunohistochemistry, fluorescence in situ hybridization, and plasma NGS were used as supplements.

Results: Among 208 mNSCLC cases with limited tissue (cohort 1), molecular genotyping using single-tissue NGS failed in 42 (20.2%) and actionable alterations were identified in only 112 of 208 cases (53.8%). In comparison, the optimized workflow in 1184 additional mNSCLC cases with limited tissue (cohort 2) increased the discovery rate of actionable alterations from 59.7% detected by tissue NGS to 70.4%. It was because that driver alterations were identified using amplification refractory mutation system polymerase chain reaction plus immunohistochemistry or fluorescence in situ hybridization in 53 of 78 (67.9%) tissue NGS-failed cases, and using plasma NGS in 73 of 143 (51.0%) tissue NGS-failed cases, which led to matched targeted therapies in 57 cases with clinical response. Moreover, the median turnaround time of the optimized workflow was significantly shorter than that of repeated biopsy for tissue NGS ( < 0.001).

Conclusions: The optimized workflow can improve mutation detection and may avoid repeated biopsy, thus allowing the timely initiation of targeted therapies for patients with newly diagnosed mNSCLC.
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http://dx.doi.org/10.1016/j.jtocrr.2021.100167DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8474491PMC
May 2021

The Reproducibility of Histopathologic Assessments of Programmed Cell Death-Ligand 1 Using Companion Diagnostics in NSCLC.

JTO Clin Res Rep 2021 Feb 5;2(2):100102. Epub 2020 Oct 5.

Department of Pathology, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People's Republic of China.

Introduction: Accurate results on the status of programmed cell death-ligand 1 (PD-L1) rely on not only the quality of immunohistochemistry testing but also the accuracy of the pathologic assessments. We explored the intraobserver and interobserver reproducibility of the interpretations for the companion diagnostics, the Dako PD-L1 22C3 pharmDx kit (Dako North America, Inc, Carpinteria, CA) and the VENTANA PD-L1 (SP263, Ventana Medical Systems, Inc, Tucson, AZ) assay, and the consistency between microscopic and digital interpretations of PD-L1.

Methods: A total of 150 surgical specimens diagnosed as NSCLC from December 2013 to July 2017 were included in this study. Twenty pathologists from different medical centers were enrolled to interpret the results of PD-L1 on the same day. A total of 100 sections were stained with the 22C3 clone and scored for the interobserver reproducibility, 20 cases of which were interpreted twice to assess the intraobserver reproducibility, and 50 cases of which were scanned into digital images to measure the consistency between microscopic and digital interpretations. A total of 44 sections were stained with the SP263 clone and scored for the interobserver reproducibility.

Results: For the intraobserver reproducibility of 22C3, the overall percent agreements were 92.0% and 89.0% for binary tumor evaluation at the cutoffs of 1% and 50%, respectively. The reliability among the pathologists revealed a substantial agreement for 22C3, whereas it revealed a substantial agreement at the cutoff of 1% and moderate agreement at the cutoffs of 25% and 50% for SP263. Microscopic and digital interpretations of PD-L1 revealed good consistency.

Conclusions: Intraobserver and interobserver reproducibility of the interpretations for PD-L1 was high using the 22C3 clone but lower for the SP263 clone. Corresponding training on such assessments, especially on the cases around the specific cutoffs, is essential for markedly improving such reproducibility. Digital imaging could improve the reproducibility of interpretation for PD-L1 among pathologists.
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http://dx.doi.org/10.1016/j.jtocrr.2020.100102DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8474465PMC
February 2021

Safety Flight Control Design of a Quadrotor UAV With Capability Analysis.

IEEE Trans Cybern 2021 Sep 29;PP. Epub 2021 Sep 29.

This article considers the safety control problem of a quadrotor unmanned aerial vehicle (UAV) subject to actuator faults and external disturbances, based on the quantization of system capability and safety margin. First, a trajectory function is constructed online with backpropagation of system dynamics. Therefore, a degraded trajectory is gracefully regenerated, via the tradeoff between the remaining system capability and the expected derivatives (velocity, jerk, and snap) of the trajectory. Second, a control-oriented model is established into a form of strict feedback, integrating actuator malfunctions and disturbances. Therefore, a retrofit dynamic surface control (DSC) scheme based on the control-oriented model is developed to improve the tracking performance. When comparing to the existing control methods, the compensation ability is analyzed to determine whether the faults and disturbances can be handled or not. Finally, simulation and experimental studies are conducted to highlight the efficiency of the proposed safety control scheme.
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http://dx.doi.org/10.1109/TCYB.2021.3113168DOI Listing
September 2021

Recent Advances in the Fabrication and Environmental Science Applications of Cellulose Nanofibril-Based Functional Materials.

Materials (Basel) 2021 Sep 17;14(18). Epub 2021 Sep 17.

College of Chemistry and Chemical Engineering, Qingdao University, Qingdao 266071, China.

Cellulose is one of the important biomass materials in nature and has shown wide applications in various fields from materials science, biomedicine, tissue engineering, wearable devices, energy, and environmental science, as well as many others. Due to their one-dimensional nanostructure, high specific surface area, excellent biodegradability, low cost, and high sustainability, cellulose nanofibrils/nanofibers (CNFs) have been widely used for environmental science applications in the last years. In this review, we summarize the advance in the design, synthesis, and water purification applications of CNF-based functional nanomaterials. To achieve this aim, we firstly introduce the synthesis and functionalization of CNFs, which are further extended for the formation of CNF hybrid materials by combining with other functional nanoscale building blocks, such as polymers, biomolecules, nanoparticles, carbon nanotubes, and two-dimensional (2D) materials. Then, the fabrication methods of CNF-based 2D membranes/films, three-dimensional (3D) hydrogels, and 3D aerogels are presented. Regarding the environmental science applications, CNF-based nanomaterials for the removal of metal ions, anions, organic dyes, oils, and bio-contents are demonstrated and discussed in detail. Finally, the challenges and outlooks in this promising research field are discussed. It is expected that this topical review will guide and inspire the design and fabrication of CNF-based novel nanomaterials with high sustainability for practical applications.
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http://dx.doi.org/10.3390/ma14185390DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8469206PMC
September 2021

Synthesis and Structure of Water-Soluble Sb Quantum Dots and Enhanced Corrosion Inhibition Performance and Mechanisms.

Inorg Chem 2021 Nov 27;60(21):16346-16356. Epub 2021 Sep 27.

Research Center of Material and Chemical Engineering, School of Material and Chemical Engineering, Tongren University, Tongren 554300, People's Republic of China.

Generally, single organic or inorganic inhibitors could effectively inhibit corrosion for metallic materials; however, there are rarely reports about the hybrid corrosion inhibitors consisting of organic and inorganic inhibitors. Thus, in this work, we synthesize a hybrid environment-friendly water-soluble corrosion inhibitor (Sb quantum dots) containing Sb, SbO, SbO, and carbon using the electrochemical exfoliation method. The inhibition effectiveness in short- and long-term immersion tests is measured using electrochemical methods, weight loss, and surface analysis. The results exhibit that the corrosion inhibition efficiency sensitively relates to the concentration of Sb quantum dots (SQDs), which achieves the largest value as the concentration of SQDs increases to 200 mg/L. Atomic force microscopy, scanning electron microscopy, and contact angle analysis reveal that the SQDs well-disperse on and cover the Q235 steel surface at 200 mg/L. According to the Langmuir adsorption data, the physicochemical adsorption and effective antioxidation of SQDs on the passivated Q235 steel surface are in charge of the effective corrosion inhibition efficiency in 0.5 M HSO solution.
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http://dx.doi.org/10.1021/acs.inorgchem.1c02172DOI Listing
November 2021
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