Publications by authors named "Lei Geng"

130 Publications

An Alternative Technique for Transumbilical Single-Port Laparoscopic Percutaneous Precise Closure of the Inguinal Hernia Sac in Children: A 3-Year Single-Centre Study.

Gastroenterol Res Pract 2021 25;2021:6679519. Epub 2021 Jun 25.

Department of Pediatric Surgery, Binzhou Medical University Hospital, Binzhou, Shandong Province, China.

Objective: To evaluate the safety and reliability of a novel technique of single-port laparoscopic-assisted percutaneous precise closure of the inguinal hernia sac in children.

Methods: From September 2016 through September 2019, children with inguinal hernia(s) treated with single-port laparoscopic-assisted percutaneous extraperitoneal closure using a guide wire were enrolled in this study. Operative time, surgical complications, recurrence rate, and cosmetic results were collected.

Results: A total of 917 cases with inguinal hernia(s) were collected. Among them, there were 886 (96.61%) boys and 31 girls. Their mean age was 5.2 ± 3.7 years. There were 693 (75.57%) cases with unilateral hernia. There were 224 cases with bilateral hernias or patent processus vaginalis, including 135 (14.72%) cases with an open contralateral ring which was confirmed intraoperatively. Twenty-three (2.51%) needed another port to complete the hernia sac separation. The operation time was 24.7 ± 5.2 min and 14.6 ± 3.8 min in bilateral and unilateral ones. Three cases complained of numbness in the thigh region or groin pain which subsided without medication in the 2 postoperative month. There was no recurrence, and the incision scars were nearly invisible.

Conclusion: Single-port laparoscopic-assisted percutaneous extraperitoneal closure using a guide wire is a safe, less cost, and reliable technique in the treatment of inguinal hernia in children.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2021/6679519DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8253622PMC
June 2021

Measurement of operative femoral anteversion during cementless total hip arthroplasty and influencing factors for using neck-adjustable femoral stem.

J Orthop Surg Res 2021 May 31;16(1):353. Epub 2021 May 31.

Medical School of Chinese PLA, Beijing, 100853, China.

Background: Placement of femoral stem in excessive anteversion or retroversion can cause reduced range of motion, prosthetic impingement, and dislocation. The aim of this study was to assess the operative femoral anteversion in patients treated with total hip arthroplasty (THA) and analyze the need of adjusting stem anteversion.

Methods: We retrospectively included 101 patients (126 hips) who underwent cementless THA with a manual goniometer to determine the femoral anteversion between October 2017 and December 2018. The operative femoral anteversion we measured was recorded during THA. We further divided those hips into three subgroups based on the range of operative femoral anteversion: group 1 (<10°), group 2 (10-30°), and group 3 (>30°) and compared the differences of their demographic data. Univariate and multivariate logistic regression were used to identify the influencing factors for the need of neck-adjustable femoral stem. The clinical and radiographic outcomes were also assessed. Perioperative complications were recorded.

Results: After THA, the Harris hip scores improved from 52.87 ± 15.30 preoperatively to 90.04 ± 3.31 at the last follow-up (p < 0.001). No implant loosening, stem subsidence, and radiolucent lines were observed on radiographs. No severe complications occurred and no components needed revision at the latest follow-up. The mean operative femoral anteversion was 14.21° ± 11.80° (range, -9 to 60°). Patients with femoral anteversion more than 30° were about 10 years younger than others. Femoral anteversion >30° was more common in patients with developmental dysplasia of the hip (DDH). There were totally 14 hips treated with the neck-adjustable femoral stem. From the univariate analysis, we can observe that female sex, diagnosis of DDH (compared with osteonecrosis), and higher operative femoral anteversion and its value >30° (compared with <10°) are associated with higher rates of using the neck-adjustable femoral stem. However, all these factors were no longer considered as independent influencing factors when mixed with other factors.

Conclusions: This study highlighted the significance of operative femoral anteversion. Identification of abnormal femoral anteversion could assist in adjusting stem anteversion and reduce the risk of dislocation after THA.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13018-021-02506-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8165773PMC
May 2021

Gut microbiome modulates Drosophila aggression through octopamine signaling.

Nat Commun 2021 05 11;12(1):2698. Epub 2021 May 11.

State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Province Key Laboratory of Biotechnology of Chinese Traditional Medicine, National & Local Joint Engineering Research Center of High-throughput Drug Screening Technology, School of life science, Hubei University, Wuhan, China.

Gut microbiome profoundly affects many aspects of host physiology and behaviors. Here we report that gut microbiome modulates aggressive behaviors in Drosophila. We found that germ-free males showed substantial decrease in inter-male aggression, which could be rescued by microbial re-colonization. These germ-free males are not as competitive as wild-type males for mating with females, although they displayed regular levels of locomotor and courtship behaviors. We further found that Drosophila microbiome interacted with diet during a critical developmental period for the proper expression of octopamine and manifestation of aggression in adult males. These findings provide insights into how gut microbiome modulates specific host behaviors through interaction with diet during development.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41467-021-23041-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8113466PMC
May 2021

Systematic Assessment of Safety Threshold for Donor Age in Cadaveric Liver Transplantation.

Front Med (Lausanne) 2021 4;8:596552. Epub 2021 Mar 4.

Division of Hepatobiliary and Pancreatic Surgery, Department of Surgery, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.

Donor age affects allograft quality and the prognosis of recipients after liver transplantation (LT). Clinicians have assessed the quality of grafts from older donors based on their appearance and texture, with no reliable quantitative evidence. Our study aimed to assess the quantitative impact of donor age on post-transplant outcomes and its safety threshold for LT, based on the published literature. Relevant studies were retrieved from the Embase, PubMed, and ISI Web of Science databases. Pooled dichotomous relative risks (RRs) were calculated using metan. Continuous RRs were calculated using a two-stage random-effects model. Eleven studies including 30,691 LT cases were included for further analysis. For categorical comparison, the RR of death within the first post-transplant year was significantly higher among patients who received grafts from older donors. Similarly, the RR of graft failure (GF) was increased within the 3 years after transplantation. For continuous comparison, advanced donor age affected transplant outcomes in a linear manner ( > 0.05). A 10-year increment in donor age was associated with RRs 1.10, 1.12, 1.15, 1.10, and 1.08 for 90-day, 180-day, 1-year, 3-year, and 5-year patient mortality and 1.08, 1.06, 1.10, 1.11, and 1.12, for 90-day, 180-day, 1-year, 2-year, and 3-year GF, respectively (all < 0.05). A spline model showed that transplants using grafts from donors <43 years old were not associated with age-related risks ( > 0.05). The risk of GF was increased in subgroups with fewer LT cases, longer cold ischemic time, fewer male donors, and recipients with viral hepatitis ( < 0.05). Donor age might affect post-LT outcomes in a dose-dependent manner. The safety threshold for donor age in terms of GF should be lowered to 43 years as an early warning for the guarantee of satisfactory outcomes. Clinicians should weigh the benefits against the risks carefully for patients receiving grafts from older donors. Further studies are warranted to investigate the mechanisms responsible for the relationship between donor age and graft quality.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fmed.2021.596552DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7969668PMC
March 2021

Internal hernia through hepatic falciform ligament iatrogenic defect in a neonate: A case report and review of the literature.

Afr J Paediatr Surg 2021 Apr-Jun;18(2):114-116

Department of Pediatric Surgery, Binzhou Medical University Hospital, Binzhou, Shandong, China.

Internal hernia through an iatrogenic defect in the hepatic falciform ligament and acquired jejunal atresia in a 8-day-old neonate was reported. The PubMed, MEDLINE, CNKI, Wanfang and Weipu databases were searched The literature about the hepatic falciform ligament iatrogenic defect causing internal hernia was analysed. Ten other cases were collected from the world literature. Herniated intestinal necrosis was found in four cases. All cases were recovered uneventfully after operation. Internal herniation through an iatrogenic defect in the hepatic falciform ligament is extremely rare. However, the case reports are increasing, especially in the era of laparoscopic surgery. Adequate closure or open the defect is essential to prevent internal hernia occurrence.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.4103/ajps.AJPS_14_20DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8232365PMC
June 2021

The Clinical Manifestation Variety and Management Choice of Meckel's Diverticulum with Complication: A Single Center Experience.

Gastroenterol Res Pract 2021 8;2021:6640660. Epub 2021 Feb 8.

Department of Pathology, Binzhou Medical University Hospital, Binzhou, Shandong 256603, China.

Background: The study was to analyze the clinical manifestation variety and management choices of symptomatic Meckel's diverticulum in children.

Methods: From July 2008 to October 2018, 28 cases of Meckel's diverticulum with a variety of complications were admitted to our hospital. The clinical data included age, gender, symptoms and signs, investigations, intraoperative and pathological findings, and outcome.

Results: The ratio of males to females was 2.5 : 1. The diagnoses were made by Tc-pertechnetate scan (in 5 cases) and by exploratory laparotomy (in 2 cases). The initial diagnosis in the other cases includes intussusception (in 4 cases), acute appendicitis (in 5 cases), intestinal obstruction (unknown origin), peritonitis, and even shock in 12 cases. Laparoscopic surgery was performed in 8 cases; 18 cases underwent open surgery. Excision of partial bowel segment with diverticulum and primary anastomosis was done in 22 cases and wedge resection of diverticulum in 4 cases. Two other cases received nonoperative therapy and went to other hospitals to receive surgical management. Ectopic gastric mucosa in the diverticulum was found in 9 cases, including 6 cases with lower gastrointestinal bleeding.

Conclusion: The clinical characteristics of Meckel's diverticulum varied. Children with hematochezia, peritonitis, and intestinal obstruction without history of prior abdominal operation should be suspected with this disease until proven otherwise. Hematochezia is often associated with ectopic gastric mucosa in the diverticulum. Laparoscopic surgery should be one of the choices for the diagnosis and treatment of Meckel's diverticulum with complications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2021/6640660DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7886519PMC
February 2021

Incomplete multi-modal representation learning for Alzheimer's disease diagnosis.

Med Image Anal 2021 04 1;69:101953. Epub 2021 Jan 1.

School of Biomedical Engineering, Shanghai Tech University, Shanghai, China; Shanghai United Imaging Intelligence Co., Ltd., Shanghai, China; Department of Artificial Intelligence, Korea University, Seoul 02841, Republic of Korea. Electronic address:

Alzheimers disease (AD) is a complex neurodegenerative disease. Its early diagnosis and treatment have been a major concern of researchers. Currently, the multi-modality data representation learning of this disease is gradually becoming an emerging research field, attracting widespread attention. However, in practice, data from multiple modalities are only partially available, and most of the existing multi-modal learning algorithms can not deal with the incomplete multi-modality data. In this paper, we propose an Auto-Encoder based Multi-View missing data Completion framework (AEMVC) to learn common representations for AD diagnosis. Specifically, we firstly map the original complete view to a latent space using an auto-encoder network framework. Then, the latent representations measuring statistical dependence learned from the complete view are used to complement the kernel matrix of the incomplete view in the kernel space. Meanwhile, the structural information of original data and the inherent association between views are maintained by graph regularization and Hilbert-Schmidt Independence Criterion (HSIC) constraints. Finally, a kernel based multi-view method is applied to the learned kernel matrix for the acquisition of common representations. Experimental results achieved on Alzheimers Disease Neuroimaging Initiative (ADNI) datasets validate the effectiveness of the proposed method.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.media.2020.101953DOI Listing
April 2021

Hydrogen-Bond-Assisted Alignment of [MCu(SeO)Cl(HO)] (M = Fe, Ga) Anionic Layers to Form Two Polar Oxychlorides: PbMCu(SeO)Cl(HO).

Inorg Chem 2021 Jan 30;60(2):831-839. Epub 2020 Dec 30.

College of Physics and Physical Engineering, Qufu Normal University, Qufu 273165, China.

Two novel selenite oxychlorides PbMCu(SeO)Cl(HO) (M = Fe, Ga) were hydrothermally synthesized and structurally characterized. They are isostructural and crystallize in the two-dimensional [MCu(SeO)Cl(HO)] anionic layer structure mediated with hydrogen bonds and aligned between neighboring layers which assist in building the three-dimensional framework with a polar space group. Optical properties measurements revealed that the optical band gaps are 2.61 and 3.22 eV for PbFeCu(SeO)Cl(HO) () and PbGaCu(SeO)Cl(HO) () and the SHG responses are about 0.12 and 0.18 times that of KDP, respectively. Furthermore, exhibits an interesting metamagnetic phenomenon under varied applied fields from around 1 to 4 T at 2 K, and behaves with potential ferromagnetic ordering at low temperature.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.inorgchem.0c02868DOI Listing
January 2021

Experience With Anti-PD-1 Antibody, Camrelizumab, Monotherapy for Biliary Tract Cancer Patients and Literature Review.

Technol Cancer Res Treat 2020 Jan-Dec;19:1533033820979703

Division of Hepatobiliary and Pancreatic Surgery, Department of Surgery, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Background: Novel immunotherapy is one of the options for advanced biliary tract cancer (BTC) patients who are traditionally intolerant to chemotherapy. However, clinical evidence for single immunotherapy with pembrolizumab or nivolumab is limited. The present study assessed the safety and efficiency of the anti-PD-1 antibody, camrelizumab, as monotherapy in patients with unresectable or recurrent BTC.

Methods: A retrospective evaluation was conducted among 4 patients with BTC, including 2 with intrahepatic cholangiocellular carcinoma (ICC), one with extrahepatic bile duct cancer, and one with gallbladder cancer. The patients with unresectable or recurrent BTC were refractory or intolerant to gemcitabine plus cisplatin treatment regimens and received at least one intravenous dose (3 mg/kg) of camrelizumab monotherapy every 3 weeks. Gene sequencing analysis was also performed for biomarker screening. Patient reaction was evaluated according to modified response evaluation criteria in solid tumor (RECIST) version 1.1, progression-free survival (PFS), and toxicity.

Results: In this cohort, 1 patient with recurrent ICC had a positive response to treatment, with a substantial tumor size reduction in liver and lung metastases verified using a radiological test after receiving 3 cycles of camrelizumab. The PFS was 4.9 months. The remaining 3 patients showed no response to treatment and experienced disease progression. RNA sequence analysis didn't found high expression on genes that related to PD-L1, microsatellite instability, tumor mutation burden, and DNA mismatch repair in these patients. Grade 3 treatment-related adverse event was observed in 1 patient.

Conclusions: Anti-PD-1 antibody camrelizumab had a manageable safety profile in patients with advanced BTC. This initial assessment of camrelizumab monotherapy provides effective evidence for patients with refractory BTC in biomarker-unselected patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1177/1533033820979703DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7739105PMC
December 2020

Metabonomic Profile of Macrosteatotic Allografts for Orthotopic Liver Transplantation in Patients With Initial Poor Function: Mechanistic Investigation and Prognostic Prediction.

Front Cell Dev Biol 2020 28;8:826. Epub 2020 Aug 28.

Division of Hepatobiliary and Pancreatic Surgery, Department of Surgery, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.

Background: Our previous study revealled amplified hazardous effects of macrosteatosis (MaS) on graft failure (GF) in recipients with severe liver damage in short post-operative days, with vague mechanism inside.

Aim: We aimed to uncover the molecular mechanism of donor MaS on GF, and construct the predictive model to monitor post-transplant prognosis based on "omics" perspective.

Methods: Ultra-performance liquid chromatography coupled to mass spectrometry metabolomic analysis was performed in allograft tissues from 82 patients with initial poor function (IPF) from multi-liver transplant (LT) centers. Pathway analysis was performed by on-line toolkit Metaboanalyst (v 3.0). Predictive model was constructed based on combinative metabonomic and clinical data extracted by stepwised cox proportional analysis.

Results: Principle component analysis (PCA) analysis revealled stratification on metabolic feature in organs classified by MaS status. Differential metabolits both associated with MaS and GF were significantly enriched on pathway of glycerophospholipid metabolism ( < 0.05). Phosphatidylcholine (PC) and phosphatidylethanolamine (PE) involved in glycerophospholipid metabolism was significantly decreased in cases with MaS donors and GF ( < 0.05). Better prediction was observed on graft survival by combinative model (area under the curve = 0.91) and confirmed by internal validation.

Conclusion: Metabonomic features of allografts can be clearly distinguished by MaS status in patients with IPF. Dysfunction on glycerophospholipid metabolism was culprit to link donor MaS and final GF. Decrement on PC and PE exerted the fatal effects of MaS on organ failure. Metabonomic data might help for monitoring long-term graft survival after LT.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fcell.2020.00826DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7484052PMC
August 2020

Association of serum osteocalcin levels with glucose metabolism in trauma patients.

Medicine (Baltimore) 2020 Sep;99(36):e21901

Department of Emergency Medicine, Shanghai JiaoTong University Affiliated Sixth People's Hospital, Shanghai, China.

Osteocalcin (OC) is an endocrine hormone that regulates glucose metabolism.The aim of this study was to investigate the relationship between serum OC levels and glucose metabolism after trauma.This was a retrospective study of trauma patients admitted to the Department of Emergency Medicine between October 2017 and April 2019. Age, height, weight, injury severity score, and previous medical history were recorded. Serum N-terminal mid-fragment of OC (N-MID OC), hemoglobin Alc (HbA1c), fasting plasma glucose (FPG), fasting insulin (FINS), C-peptide, and other biochemical indicators were measured. Differences between the HbA1c-L (HbA1c <6.5%) and HbA1c-H (HbA1c ≥6.5%) groups were compared. The association of N-MID OC with indicators of glucose metabolism was analyzed.Out of 394 trauma patients, leukocyte and FPG levels in the HbA1c-H group (n = 93) were higher (P < .05), while N-MID OC levels were lower (P = .011) than the HbA1c-L group (n = 301). N-MID OC was negatively correlated with HbA1c in the total population (r = -0.273, P < .001) as well as in the HbA1c-L (r = -0.289, P < .001) and HbA1c-H (r = -0.390, P < 0.001) groups, and was positively correlated with C-peptide in the HbA1c-H group (r = 0.395, P < .001). The different quartiles in the HbA1c-L showed that N-MID OC declined with increasing HbA1c, which was higher than N-MID OC levels in the HbA1c-H group. Multiple linear regression analysis revealed that serum HbA1c was independently associated with serum OC levels after trauma (β=-1.608, P < .001).This study strongly suggests the importance of serum OC on glucose metabolism in trauma patients. HbA1c is independently associated with serum OC levels.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/MD.0000000000021901DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7478502PMC
September 2020

Loss of control of the culturable bacteria in the hindgut of Bombyx mori after Cry1Ab ingestion.

Dev Comp Immunol 2020 10 26;111:103754. Epub 2020 May 26.

Key Laboratory of Insect Developmental and Evolutionary Biology, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, China; Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, 100093, China. Electronic address:

Bt protein, produced by Bacillus thuringiensis, can bind receptors to destroy the physiological functions of the insect midgut. It is unknown whether Bt can also target the hindgut and influence its defense against fecal bacteria. Here we show that Crystal protein 1Ab (Cry1Ab), a Bt protein, was detected in the larval hindgut contents of Bombyx mori after ingestion of this toxin protein. The number of fecal bacteria that can be inhibited by the hindgut prophenoloxidase-induced melanization was significantly enhanced after oral ingestion of Cry1Ab. Although the hindgut contents became brown, the activity of hindgut phenoloxidase was decreased. LC-MS/MS analysis of the hindgut lumen contents revealed that many new proteins including several proteases were newly secreted. The enhanced secretion of proteases cleaved prophenoloxidase to decrease its activity, including the corresponding activity to inhibit the fecal bacteria. In addition, after ingestion of Cry1Ab, the pylorus (between the midgut and hindgut) could not autonomously contract due to the physical detachment of the acellular cuticle-like membrane from the epidermal cells, which prevented the movement of food from the midgut to the hindgut. Some cells in the cryptonephry of the hindgut became swollen and degraded, possibly due to the presence of Cry1Ab in the hindgut. These findings demonstrate that the inhibition of feces bacteria by the hindgut prophenoloxidase-induced melanization is out of control after Cry1Ab ingestion.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.dci.2020.103754DOI Listing
October 2020

Identification of HO-1 as a novel biomarker for graft acute cellular rejection and prognosis prediction after liver transplantation.

Ann Transl Med 2020 Mar;8(5):221

Division of Hepatobiliary and Pancreatic Surgery, Department of Surgery, First Affiliated Hospital, School of Medicine, Zhejiang University, College of Medicine, Hangzhou 310003, China.

Background: Liver transplantation (LT) is the most effective treatment for patients with end-stage liver diseases, but acute rejection is still a major concern. However, the mechanisms underlying rejection remain unclear. Biomarkers are lacking for predicting rejection and long-term survival after LT.

Methods: Isobaric tags for relative and absolute quantitation (iTRAQ)-based proteomics was performed between acute cellular rejection (ACR) and non-rejection recipients. The molecular signature differences and potential biomarkers were identified by comprehensive bioinformatics. Heme oxygenase-1 (HO-1) expression and its association with clinical outcomes were investigated by tissue microarrays consisted of liver specimens from recipients with (n=80) and without ACR (n=57).

Results: A total of 287 differentially expressed proteins (DEPs) were identified. Pathway analysis revealed that T/B cell activation, integrin/inflammation signaling pathway, etc. were significantly correlated with ACR. Through comprehensive bioinformatics, HO-1 was identified as a candidate potential biomarker for ACR. In tissue microarray (TMA) analysis, HO-1 expression was significantly higher in ACR group than in non-rejection group (P<0.01). Preoperative Child-Pugh and Meld scores were significantly higher in recipients with high HO-1 expression (P<0.01). In a mean 5-year follow-up, recipients with high HO-1 expression were associated with a shorter overall survival (P<0.05). Further multivariate analyses indicated that HO-1 could be an independent adverse prognostic factor for post-transplant survival (P=0.005).

Conclusions: A total of 287 DEPs were identified, providing a set of targets for further research. Recipients with high preoperative HO-1 expression were associated with ACR. HO-1 may be used as a potential biomarker for predicting the development of post-transplant allograft ACR and recipient's survival.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.21037/atm.2020.01.59DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7154463PMC
March 2020

Hydrogen-rich saline protects intestinal epithelial tight junction barrier in rats with intestinal ischemia-reperfusion injury by inhibiting endoplasmic reticulum stress-induced apoptosis pathway.

J Pediatr Surg 2020 Dec 22;55(12):2811-2819. Epub 2020 Feb 22.

Department of Pediatric Surgery, Binzhou Medical University Hospital, Binzhou 256600, Shandong, China.

Aim: To investigate the effects of hydrogen-rich saline (HRS) on intestinal epithelial tight junction (TJ) barrier in rats with intestinal ischemia-reperfusion injury (IIRI).

Materials And Methods: Thirty-two healthy male Sprague-Dawley (SD) rats were randomly divided into four groups (n = 8 each): Sham group, I/R group, HRS group and 4-PBA group. After 45 min of ischemia and 6 h of reperfusion, the rats were sacrificed to collect serum and ileum for detection. Hematoxylin and eosin (H&E) staining was used to observe the morphology of small intestine. The serum expression levels of intestinal fatty acid binding protein (IFABP), tumor necrosis factor-α (TNF-α), and interleukin-1β (IL-1β) were determined by enzyme linked immunosorbent assay (ELISA). Imunohistochemistry, immunofluorescence and Western blot were used to detect key proteins in intestinal epithelial TJs, ERS, and ERS-induced apoptosis, including occludin, zonula occludens-1 (ZO-1), glucose-regulated protein 78 (GRP78), X-box binding protein-1 (XBP1), C/EBP-homologous protein (CHOP) and caspase-3. Data was presented as mean ± SEM and compared using one-way ANOVA. A p-value <0.05 was considered significant.

Results: Compared with rats in the I/R group, the Chiu score of ileum damage in the HRS group and 4-PBA group were lower. The levels of serum IFABP, TNF-α, and IL-1β were statistically significant among the groups. Increased expression of TJ proteins occludin and ZO-1 by reducing various parameters of ERS and ERS-induced apoptosis evidenced by down-regulation of the protein levels of GRP78, XBP1, CHOP and caspase-3 were shown in the HRS and 4-PBA groups.

Conclusion: HRS had potential protective effects on intestinal barrier in IIRI rats. This study suggested that inhibition of excessive ERS and ERS-induced apoptosis by HRS may reduce intestinal epithelial cells damage and maintain the integrity of intestinal epithelial TJ barrier in rats with IIRI.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jpedsurg.2020.01.061DOI Listing
December 2020

Pluripotency factor NANOG promotes germ cell maintenance in vitro without triggering dedifferentiation of spermatogonial stem cells.

Theriogenology 2020 May 21;148:68-75. Epub 2020 Feb 21.

State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, Jiangsu, 210029, China. Electronic address:

Spermatogonial stem cells (SSCs) are defined as the tissue-specific stem cells in the testes that produce sperm and support life-long spermatogenesis. A culture microenvironment could convert mouse SSCs from unipotent to pluripotent states; however, the underlying mechanism is unclear. NANOG has been considered the decisive transcriptional factor for pluripotency transition of stem cells, but NANOG is not expressed in SSCs. Here, we investigated whether NANOG overexpression could result in SSCs being converted into a pluripotent state. We found that rare NANOG-positive cells could be detected in spermatogonia, pachytene spermatocytes, and even round spermatids in mouse testes, and that the induction of NANOG could promote the proliferation of cultured SSCs in vitro and partially compensate for the role of the growth factor GDNF. In vivo allogeneic transplantation of NANOG-overexpressing germ cells did not yield any teratoma-like tissues, but regenerated normal colonies of spermatogenesis in the testes of recipient mice. Collectively, our data showed that overexpression of the pluripotency factor NANOG along did not dedifferentiate testis germline stem cells into a pluripotent state, suggesting that other genetic or epigenetic factors are involved in SSC reprogramming.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.theriogenology.2020.02.028DOI Listing
May 2020

Modern instant messaging platform for postoperative follow-up of patients after total joint arthroplasty may reduce re-admission rate.

J Orthop Surg Res 2019 Dec 27;14(1):464. Epub 2019 Dec 27.

Department of Orthopedics, Chinese PLA General Hospital, 28 Fuxing Road, Beijing, 100853, China.

Background: Follow-up after artificial joint replacement greatly helps achieve surgical outcomes. Mobile internet technology and mobile terminal equipment may increase the effectiveness of artificial joint replacement. However, only a few studies have evaluated the effectiveness of this technology. We aimed to analyze the reasons and outcomes of patients who used the instant messaging platform after undergoing artificial joint replacement.

Methods: Among the 548 cases of arthroplasty (250 hips, 298 knees) performed between December 2015 and June 2018 in the Department of Joint Surgery of our institution; 358 (164 hip joints, 194 knee joints) participated in instant messaging platform consultation, whereas the remaining 190 (86 hip joints, 104 knee joints) participated in traditional telephone consultation, as a control group. Follow-up time was from December 2015 to August 2018 (follow-up period was 2-32 months). Data on age, sex, type of surgery, date of surgery, date of discharge, and length of hospital stay were collected from electronic medical records.

Results: We analyzed the consultation contents of 358 patients who participated in instant messaging platform consultation. Counseling was mainly related to pain (13.6%), appointment review (12.4%), activity problems (10.5%), and incision problems (8.9%). Most problems were resolved through online guidance, with 8.4% of patients requiring only outpatient treatment and 2.5% of patients requiring rehospitalization. A total of 190 patients were followed up through traditional telephone consultation; 6.8% of patients required outpatient department treatment and 7.4% were eventually re-admitted.

Conclusion: The instant messaging platform consultation service effectively informs patients of potential postoperative problems and helps resolve them. It allows early detection and management of postoperative adverse events, including problems related to medication, wound, and activity, thereby effectively reducing readmission rate.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13018-019-1407-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6935083PMC
December 2019

Galectin-1 attenuates hepatic ischemia reperfusion injury in mice.

Int Immunopharmacol 2019 Dec 21;77:105997. Epub 2019 Nov 21.

Key Laboratory of Combined Multi-organ Transplantation, Ministry of Public Health, Department of Hepatobiliary and Pancreatic Surgery, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, China. Electronic address:

Background: Hepatic ischemia reperfusion injury (IRI) is a primary cause of organ dysfunction occurring during liver resection surgery and transplantation. Galectin-1, an endogenous lectin expressed on lymphoid organs, plays an important role in governing innate and adaptive immunity. This study was designed to determine the therapeutic role of galectin-1 and underlying mechanism in hepatic IRI.

Methods: Male C57BL/6 mice were subjected to 90 min of partial hepatic ischemia followed by reperfusion with or without treatment with recombinant galectin-1 (rGal-1) or neutralizing anti-IL-10 antibody. Mice were sacrificed at 6 and 24 h following reperfusion. Liver damage related enzymes were determined and cytokines/chemokines were measured by qPCR and ELISA.

Results: Administration of rGal-1 significantly attenuated hepatic IRI, including a remarkable reduction in serum ALT/AST levels and an improved liver histology score compared to controls. rGal-1 treatment reduced TUNEL positive apoptotic hepatocytes, attenuated proinflammatory cytokines (TNF-α, IL-6, IL-1β, IL-12, IFN-γ, IL-17) and chemokines (CXCL-1, CXCL-10) levels, but upregulated IL-10 expression, compared with controls. In addition, rGal-1 increased the production of IL-10 in hepatic macrophages in vivo and in vitro. Blockade of IL-10 using neutralizing anti-IL-10 antibody reversed the protection of galectin-1 in hepatic IRI in mice.

Conclusion: These data suggest that galectin-1 may attenuate hepatic IRI via an IL-10-dependent mechanism, which is a promising therapeutic target.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.intimp.2019.105997DOI Listing
December 2019

Alternative technique to save ischemic bowel segment in management of neonatal short bowel syndrome: A case report.

World J Clin Cases 2019 Oct;7(20):3353-3357

Department of Pediatric Surgery, Binzhou Medical University Hospital, Binzhou 256603, Shandong Province, China.

Background: Congenital short bowel syndrome (SBS) associated with malrotation, gut volvulus and jejuno-ileal atresia is a very rare condition. It is a severe challenge for surgeons to preserve residual ischemic bowel segment in the management of short bowel syndrome,especially in neonates.

Case Summary: We report a newborn baby with gut malrotation associated with jejuno-ileal atresia, congenital SBS and jejunal volvulus. Hematemesis and abdominal distention were noted. At laparotomy, malrotation associated with jejuno-ileal atresia, congenital SBS and jenunal volvulus was confirmed. The total length of the small bowel was 63 cm with proximal jejunal bowel segment measuring 38 cm, including 18 cm necrotic segment below the Treitz's ligament and 20 cm severe ischemic segment. The distal part of the small bowel was 25 cm in length and only about 0.8 cm in diameter. Ladd's procedure, necrotic segment resection and end-to-back duodeno-ileal anastomosis were performed. The residual severe ischemic jejunum was preserved with single proximal stoma and distal end closure. Three months later, to restore the continuity of the isolated gut segment, end-to-end duodeno-jejunal and jejuno-ileal anastomosis was performed. The entire functional small bowel length increased to 80 cm. Intravenous fluid therapy and parenteral nutrition were discontinued on the 10 day postoperatively. Twelve months later, her body weight was 9.5 kg.

Conclusion: Isolation of severe ischemic bowel segment and staged anastomosis to restore the gut continuity for infants with SBS are safe and feasible.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.12998/wjcc.v7.i20.3353DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6819291PMC
October 2019

[Corrigendum] Microarray analysis of the molecular mechanisms associated with age and body mass index in human meniscal injury.

Mol Med Rep 2019 10 30;20(4):3968. Epub 2019 Aug 30.

Department of Orthopedic Surgery, Shanghai Fifth People's Hospital Affiliated to Fudan University, Shanghai 200240, P.R. China.

Subsequently to the publication of the article, the authors have realized that the grant number in their paper in the Funding section was presented incorrectly: "2017MH77" should have been written as "2017MHZ77". Therefore, the funding information in the declarations section should have appeared as follows: "The present study was funded by the Minhang District Natural Science Project (grant no. 2017MHZ77)". The authors regret that this error was not noticed prior to the publication of their paper, and apologize for any inconvenience caused. [the original article was published in Molecular Medicine Reports 19: 93‑102, 2019; DOI: 10.3892/mmr.2018.9685].
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3892/mmr.2019.10629DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6755175PMC
October 2019

Encoder-decoder with dense dilated spatial pyramid pooling for prostate MR images segmentation.

Comput Assist Surg (Abingdon) 2019 10 19;24(sup2):13-19. Epub 2019 Aug 19.

Tianjin Key Laboratory of Optoelectronic Detection Technology and Systems , Tianjin , China.

Automatic segmentation of prostate magnetic resonance (MR) images has great significance for the diagnosis and clinical application of prostate diseases. It faces enormous challenges because of the low contrast of the tissue boundary and the small effective area of the prostate MR images. In order to solve these problems, we propose a novel end-to-end professional network which consists of an Encoder-Decoder structure with dense dilated spatial pyramid pooling (DDSPP) for prostate segmentation based on deep learning. First, the DDSPP module is used to extract the multi-scale convolution features in the prostate MR images, and then the decoder is used to capture the clear boundary of prostate. Competitive results are produced over state of the art on 130 MR images which key metrics Dice similarity coefficient (DSC) and Hausdorff distance (HD) are 0.954 and 1.752 mm respectively. Experimental results show that our method has high accuracy and robustness.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/24699322.2019.1649069DOI Listing
October 2019

Lung segmentation method with dilated convolution based on VGG-16 network.

Comput Assist Surg (Abingdon) 2019 10 12;24(sup2):27-33. Epub 2019 Aug 12.

Tianjin Key Laboratory of Optoelectronic Detection Technology and System , Tianjin , China.

Lung cancer has become one of the life-threatening killers. Lung disease need to be assisted by CT images taken doctor's diagnosis, and the segmented CT image of the lung parenchyma is the first step to help doctor diagnosis. For the problem of accurately segmenting the lung parenchyma, this paper proposes a segmentation method based on the combination of VGG-16 and dilated convolution. First of all, we use the first three parts of VGG-16 network structure to convolution and pooling the input image. Secondly, using multiple sets of dilated convolutions make the network has a large enough receptive field. Finally, the multi-scale convolution features are fused, and each pixel is predicted using MLP to segment the parenchymal region. Experimental results were produced over state of the art on 137 images which key metrics Dice similarity coefficient (DSC) is 0.9867. Experimental results show that this method can effectively segment the lung parenchymal area, and compared to other conventional methods better.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/24699322.2019.1649071DOI Listing
October 2019

YAP promotes multi-drug resistance and inhibits autophagy-related cell death in hepatocellular carcinoma via the RAC1-ROS-mTOR pathway.

Cancer Cell Int 2019 12;19:179. Epub 2019 Jul 12.

1Division of Hepatobiliary and Pancreatic Surgery, Department of Surgery, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.

Background: Multi-drug resistance is the major cause of chemotherapy failure in hepatocellular carcinoma (HCC). YAP, a critical effector of the Hippo pathway, has been shown to contribute to the progression, metastasis and invasion of cancers. However, the potential role of YAP in mediating drug resistance remains obscure.

Methods: RT-qPCR and western blot were used to assess YAP expression in HCC cell lines. CCK-8 assays, flow cytometry, a xenograft tumour model, immunochemistry and GFP-mRFP-LC3 fusion proteins were utilized to evaluate the effect of YAP on multi-drug resistance, intracellular ROS production and the autophagy of HCC cells in vitro and in vivo. Autophagy inhibitor and rescue experiments were carried out to elucidate the mechanism by which YAP promotes chemoresistance in HCC cells.

Results: We found that BEL/FU, a typical HCC cell line with chemoresistance, exhibited overexpression of YAP. Moreover, the inhibition of YAP by shRNA or verteporfin conferred the sensitivity of BEL/FU cells to chemotherapeutic agents through autophagy-related cell death in vitro and in vivo. Mechanistically, YAP silencing significantly enhanced autophagic flux by increasing RAC1-driven ROS, which contributed to the inactivation of mTOR in HCC cells. In addition, the antagonist of autophagy reversed the enhanced effect of YAP silencing on cell death under treatment with chemotherapeutic agents.

Conclusion: Our findings suggested that YAP upregulation endowed HCC cells with multi-drug resistance via the RAC1-ROS-mTOR pathway, resulting in the repression of autophagy-related cell death. The blockade of YAP may serve as a promising novel therapeutic strategy for overcoming chemoresistance in HCC.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12935-019-0898-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6626386PMC
July 2019

Acoustic Field of a Linear Phased Array: A Simulation Study of Ultrasonic Circular Tube Material.

Sensors (Basel) 2019 May 22;19(10). Epub 2019 May 22.

School of Electronics and Information Engineering, Tianjin Polytechnic University, Tianjin 300380, China.

As ultrasonic wave field radiated by an ultrasonic transducer influences the results of ultrasonic nondestructive testing, simulation and emulation are widely used in nondestructive testing. In this paper, a simulation study is proposed to detect defects in a circular tube material. Firstly, the ultrasonic propagation behavior was analyzed, and a formulation of the Multi-Gaussian beam model (MGB) based on a superposition of Gaussian beams is described. The expression of the acoustic field from a linear phased-array ultrasonic transducer in the condition of a convex interface on the circular tube material is proposed. Secondly, in order to make the tapered probe wedge better fit the curved circular tube material and carry out the ultrasonic inspection of the curved surface, it was necessary to pare the angle probe wedge. Finally, acoustic field simulations in a circular tube were carried out and analyzed. The simulation results indicated that the method of ultrasonic phased-array inspection is feasible in circular tube testing. Tube materials with different curvatures need different array element lengths and widths to get the desired focused beam.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/s19102352DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6567204PMC
May 2019

Design and Regulation of Novel [email protected] Nanowires as High Performance Electrode for Supercapacitor.

Nanomaterials (Basel) 2019 May 21;9(5). Epub 2019 May 21.

Tianjin Key Laboratory of Advanced Functional Porous Materials, Institute for New Energy Materials & Low-Carbon Technologies, School of Materials Science and Engineering, Tianjin University of Technology, No. 391 Binshui West Street Xiqing District, Tianjin 300384, China.

Bimetallic oxides have been considered as potential candidates for supercapacitors due to their relatively high electric conductivity, abundant redox reactions and cheapness. However, nanoparticle aggregation and huge volume variation during charging-discharging procedures make it hard for them to be applied widely. In this work, one-dimensional (1D) [email protected] nanowires were in-situ synthesized via a simply modified micro-emulsion technique, followed by thermal treatment. The novel 1D and core-shell architecture, and in-situ carbon coating promote its electric conductivity and porous feature. Due to these advantages, the [email protected] electrode exhibits a high specific capacitance of 824 F·g at 0.1 A·g and remains 476 F·g at 5 A·g. After 10,000 cycles, the capacitance retention of the [email protected] electrode is up to 93.9%, suggesting its excellent long-term cycling stability. After assembling with activated carbon (AC) to form a [email protected]//AC device, the energy density of this [email protected]//AC device is 27 W·h·kg at a power density of 290 W·kg, and remains at a 10 W·h·kg energy density at a high power density of 9300 W·kg.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/nano9050777DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6566516PMC
May 2019

Lower mean platelet volume is a risk indicator of hepatocellular carcinoma recurrence following liver transplantation.

Hepatobiliary Pancreat Dis Int 2019 Jun 24;18(3):223-227. Epub 2019 Apr 24.

Division of Hepatobiliary and Pancreatic Surgery, Department of Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China; Key Laboratory of Combined Multi-Organ Transplantation, Ministry of Public Health, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China. Electronic address:

Background: Lower mean platelet volume (MPV) is an indicator of platelet activity in the setting of tumor development. This study was to assess the relationship between preoperative MPV and survival outcomes of patients with hepatocellular carcinoma (HCC) following liver transplantation (LT).

Methods: The demographic and clinical characteristics of 304 HCC patients following LT were retrieved from an LT database. All the patients were divided into the normal and lower MPV groups according to the median MPV. The factors were first analyzed using a Kaplan-Meier survival analysis, then the factors with P < 0.10 were selected for multivariate Cox regression analysis and were used to define the independent risk factors for poor prognosis.

Results: The 1-, 3-, and 5-year tumor free survival was 95.34%, 74.67% and 69.29% in the normal MPV group, respectively, and 95.40%, 59.97% and 42.94% in the lower MPV group, respectively (P < 0.01). No significant difference was observed in post-LT complications between the normal and lower MPV groups. Portal vein tumor thrombosis (PVTT) [hazard ratio (HR = 2.24; 95% confidence interval: 1.46-3.43; P < 0.01) and lower MPV (HR = 1.58; 95% confidence interval: 1.05-2.36; P = 0.03) were identified as independent prognostic risk factors for recipient survival.

Conclusion: Preoperative lower MPV is a risk indicator of HCC patients survival outcomes after LT.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.hbpd.2019.04.009DOI Listing
June 2019

[Segmentation of retinal image vessels based on fully convolutional network with depthwise separable convolution and channel weighting].

Sheng Wu Yi Xue Gong Cheng Xue Za Zhi 2019 Feb;36(1):107-115

Tianjin Polytechnic University, School of Electronics and Information Engineering, Tianjin 300387, P.R.China;Tianjin Key Laboratory of Optoelectronic Detection and Systems, Tianjin 300387, P.R.China.

Diseases such as diabetes and hypertension can lead to change the shape of the retinal blood vessels. Segmentation of fundus images is a key step in the process of quantitative analysis of the disease, which is instructive in the analysis and diagnosis of clinical diseases. In this paper, a method for the segmentation of retinal image vessels based on fully convolutional network (FCN) with depthwise separable convolution and channel weighting is presented. Firstly, CLAHE and Gamma correction of the green channel of the fundus image are used to enhance the contrast. Then, in order to adapt to network training, the enhanced image is divided into patches to expand the data. Finally, the depthwise separable convolution instead of the standard convolution method is used to increase the network width. Meanwhile, the channel weighting module is introduced to explicitly model the relationship between the characteristic channels in order to improve the distinguishability of the features. The combination of them is applied to the FCN and the results of expert manual identification are used to supervise the experiment on the DRIVE database. The results show that the segmentation accuracy of the proposed method in DRIVE database reached 0.963 0 and AUC reached 0.983 1. The segmentation accuracy in STARE database reached 0.962 0 and AUC achieved 0.983 0. To some extent, the proposed method has better feature resolution and better segmentation performance.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7507/1001-5515.201801054DOI Listing
February 2019

Super resolution reconstruction for medical image based on adaptive multi-dictionary learning and structural self-similarity.

Comput Assist Surg (Abingdon) 2019 Mar 1:1-8. Epub 2019 Mar 1.

b School of Electronics and Information Engineering , Tianjin Polytechnic University , Tianjin , China.

To improve the quality of the super-resolution (SR) reconstructed medical images, an improved adaptive multi-dictionary learning method is proposed, which uses the combined information of medical image itself and the natural images database. In training dictionary section, it uses the upper layer images of pyramid which are generated by the self-similarity of low resolution images. In reconstruction section, the top layer image of pyramid is taken as the initial reconstruction image, and medical image's SR reconstruction is achieved by regularization term which is the non-local structure self-similarity of the image. This method can make full use of the same scale and different scale similar information of medical images. Simulation experiments are carried out on natural images and medical images, and the experimental results show the proposed method is effective for improving the effect of medical image SR reconstruction.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/24699322.2018.1557906DOI Listing
March 2019

Super resolution reconstruction for medical image based on adaptive multi-dictionary learning and structural self-similarity.

Comput Assist Surg (Abingdon) 2019 10 28;24(sup1):81-88. Epub 2019 Jan 28.

School of Electronics and Information Engineering, Tianjin Polytechnic University , Tianjin , China.

To improve the quality of the super-resolution (SR) reconstructed medical images, an improved adaptive multi-dictionary learning method is proposed, which uses the combined information of medical image itself and the natural images database. In training dictionary section, it uses the upper layer images of pyramid which are generated by the self-similarity of low resolution images. In reconstruction section, the top layer image of pyramid is taken as the initial reconstruction image, and medical image's SR reconstruction is achieved by regularization term which is the non-local structure self-similarity of the image. This method can make full use of the same scale and different scale similar information of medical images. Simulation experiments are carried out on natural images and medical images, and the experimental results show the proposed method is effective for improving the effect of medical image SR reconstruction.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/24699322.2018.1560092DOI Listing
October 2019

RIPK1 Inhibition Enhances Pirarubicin Cytotoxic Efficacy through AKT-P21-dependent Pathway in Hepatocellular Carcinoma.

Int J Med Sci 2018 5;15(14):1648-1657. Epub 2018 Nov 5.

Division of Hepatobiliary and Pancreatic Surgery, Department of Surgery, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China.

Pirarubicin (THP) is a new generation cell cycle nonspecific anthracycline anticancer drug. Pirarubicin and pirarubicin-based combination therapies have been demonstrated to be effective against HCC in TACE. However, the drug resistance limits its therapeutic efficacy. Receptor-interacting protein kinase 1 (RIPK1) displays a critical role in cell death. Here we found that RIPK1 and p21 may participate in the resistance to pirarubicin. In this study, we first found that inhibition of RIPK1 significantly decreased pAKT and increased p21, accompanied by G0/G1 phase cell cycle arrest and cell anti-proliferation in pirarubicin-treated hepatocellular carcinoma cells. Moreover, phosphorylation of AKT reversed the anti-proliferative effect of RIPK1 inhibitor in HCC, which proved that RIPK1-AKT-P21-dependent pathway played a key role in pirarubicin resistance. Using a mouse xenograft model, we further found that RIPK1 inhibitor combined with pirarubicin exerted synergistic anti-tumor effect . Upon exposure to pirarubicin treatment, xenografts under RIPK1 inhibition maintained higher levels of p21 than control xenografts. In conclusion, the results in our study demonstrated that RIPK1 inhibition enhances the anti-tumor effect of pirarubicin by overcoming drug resistance. RIPK1 inhibitor might be used as an adjuvant to potentiate the inhibitory effect of pirarubicin against primary hepatocellular carcinoma.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7150/ijms.28289DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6299408PMC
April 2019

Microarray analysis of the molecular mechanisms associated with age and body mass index in human meniscal injury.

Mol Med Rep 2019 Jan 21;19(1):93-102. Epub 2018 Nov 21.

Department of Orthopedic Surgery, Shanghai Fifth People's Hospital Affiliated to Fudan University, Shanghai 200240, P.R. China.

The aim of the present study was to identify genes and functional pathways associated with meniscal injuries affected by age or body mass index (BMI) using microarray analysis. The GSE45233 gene expression dataset with 12 injured meniscus samples associated with age and BMI and GSE66635 dataset with 12 injured and 12 normal meniscus samples were downloaded from the Gene Expression Omnibus database. Differentially expressed genes (DEGs) were identified based on age or BMI in GSE45233. DEGs between injured and normal meniscus samples in GSE66635 were also identified. Common DEGs between GSE45233 and GSE66635 were identified as feature genes associated with age or BMI, followed by protein‑protein interaction (PPI) network and functional pathway enrichment analyses for the feature genes. Finally, the GSE51588 genome‑wide expression profile was then downloaded from the GEO database to validate the results. A total of 1,328 DEGs were identified. Of these, 28 age‑associated and 20 BMI‑associated meniscal injury genes were obtained. B‑cell lymphoma‑2 (Bcl‑2) and matrix metalloproteinase‑14 were identified as hub genes in the PPI networks. Functional pathway enrichment analysis revealed that vascular endothelial growth factor A (VEGFA), transferrin (TF) and Bcl‑2 were involved in the hypoxia‑inducible factor 1 signaling pathway. TF was involved in the mineral absorption function pathway associated with BMI. Additionally, TF and VEGFA were identified to be overlapping candidate genes of GSE45233 and GSE66635, and DEGs in GSE51588. Therefore, VEGFA, TF, and Bcl‑2 may be important genes for human meniscal injuries. Additional evaluations of these results are required.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3892/mmr.2018.9685DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6297773PMC
January 2019
-->