Publications by authors named "Lei Ge"

283 Publications

Combined protective effects of icariin and selenomethionine on novel chronic tubulointerstitial nephropathy models and .

Br J Nutr 2021 Mar 5:1-33. Epub 2021 Mar 5.

College of Veterinary Medicine, Nanjing Agricultural University, Nanjing210095, Jiangsu Province, China.

Chronic tubulointerstitial nephropathy (CTIN) is one of the most common kidney diseases. However, treatment for CTIN has multiple limits. Adjuvant therapy through nutritional regulation has become a hot research topic at present. Icariin (ICA), an extraction of Chinese herbal medicine epimedium, has many pharmacological functions including anti-inflammation and tonifying kidney. Selenomethionine (SeMet) possesses the effects of antioxidant and lightening nephrotoxicity. However, little is known about the combined nephroprotection of them. This study was investigated to evaluate the joint effects of ICA and SeMet on CTIN and explore the mechanism. Based on a novel CTIN model developed in our previous study, mice were randomly divided into five groups (a: control; b: model; c: model+ICA; d: model+SeMet; e: model+ICA+SeMet). Renal tubule epithelial cells were treated with cyclosporine A and ochratoxin A without/with ICA or/and SeMet. The results showed ICA or/and SeMet ameliorated CTIN by inhibiting the uptrends of blood urine nitrogen, serum creatinine, urine protein, urine gravity, histopathological damage degree and collagen Ⅰ deposition. ICA or/and SeMet also increased cell proliferation and decreased apoptosis and the expression of TGF-β1 and α-SMA. Emphatically, ICA and SeMet joint had better nephroprotection than alone in most indexes including fibrosis. Furthermore, ICA and SeMet joint decreased the activation of toll like receptor 4 (TLR4)/NFκB pathway induced by CTIN. TLR4 overexpression counteracted the joint protection of ICA and SeMet. Therefore, ICA and SeMet in combination could protect against CTIN through blocking TLR4/NFκB pathway. The study will provide novel insights to explore an adjuvant therapeutic orientation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1017/S0007114521000787DOI Listing
March 2021

Construction of Nanocrystalline Cellulose-Based Composite Fiber Films with Excellent Porosity Performances via an Electrospinning Strategy.

ACS Omega 2021 Feb 11;6(7):4958-4967. Epub 2021 Feb 11.

State Key Laboratory of Metastable Materials Science and Technology, Yanshan University, Qinhuangdao 066004, P. R. China.

Cellulose nanocrystals (CNCs) not only have environmental protection characteristics of being lightweight, degradable, green, and renewable but also have some nanocharacteristics of high strength, large specific surface area, and obvious small size effect, so they are often used as a reinforcing agent in various polymers. However, the hydrogen bonding between CNC molecules is relatively strong, and they can easily aggregate and get entangled with each other. In this work, several large-porosity composite nanofiber films, KH550-CNC/waterborne polyurethane (WPU)/poly(vinyl alcohol) (PVAL) with KH550-modified CNCs, are prepared using poly(vinyl alcohol) (PVAL) solution and electrospinning technology. A variety of characterization methods are used to discuss and analyze the nanofiber materials, and the effects of the added amount of CNCs modified with KH550, spinning voltage, curing distance, and advancing speed on the morphology and performance of composite fibers are discussed separately. The results show that when the content of KH550-CNC is 1%, the composite fiber film obtained has the most regular morphology and the best spinnability, which is convenient for the specific application of fiber materials in a later period. In addition, the porosity of the obtained composite fiber film is 62.61%. Therefore, this work provides a theoretical basis and research strategy for the preparation of higher-porosity composite films as well as the development of new textile materials.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsomega.0c06002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7905938PMC
February 2021

Nontoxic dose of Phenethyl isothiocyanate ameliorates deoxynivalenol-induced cytotoxicity and inflammation in IPEC-J2 cells.

Res Vet Sci 2021 Feb 6;136:66-73. Epub 2021 Feb 6.

College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, China; Institute of Nutritional and Metabolic Disorders in Domestic Animals and Fowls, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, China; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, China. Electronic address:

The intestinal tract is a target for the deoxynivalenol (DON), which has adverse effects in animals and humans' health by affecting intestinal functions. Phenethyl isothiocyanate (PEITC) is an important degradation product of glucosinolates (GSLs), belonging to an anti-nutritional factor that affects the digestion and absorption of nutrients in the animals' intestinal. However, little attention has been paid to the interaction and its mechanism between DON and PEITC. Therefore, the purpose of this study was to assess the effects of PEITC on DON-induced cytotoxicity and inflammation, and explore the potential mechanisms in IPEC-J2 cells. Our results showed that DON exposure could decrease the cell viability and pro-inflammatory cytokine expression in IPEC-J2 cells in a dose-dependent manner. PEITC treatment at the concentrations of 1.25-5 μM had no significant effect on IPEC-J2 cells viability, but above 10 μM of PEITC treatment significantly reduced the cell viability. Interestingly, 1.25-5 μM of PEITC treatment could suppress 4 μM of DON-induced decrease in cell viability and increase in pro-inflammatory cytokine expression. Meanwhile, the protein ratios of p-p65/p-65 and p-IκBα/IκBα were markedly decreased in the groups treated with 1.25-5 μM PEITC compared to DON exposure alone. However, the protective effects of PEITC treatment were significantly blocked after pre-treatment with LPS, NF-κB activator, in IPEC-J2 cells. In conclusion, these findings indicated that the nontoxic dose of PEITC could alleviate DON-induced cytotoxicity and inflammation responses via suppressing the NF-κB signaling pathway in IPEC-J2 cells. Our results provide a new theoretical basis for the rational addition of rapeseed meal in animal feedstuff.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.rvsc.2021.02.002DOI Listing
February 2021

Arabidopsis WXR1/3 regulate transitory starch metabolism in young seedlingscorresponding to circadian rhythm.

J Exp Bot 2021 Feb 11. Epub 2021 Feb 11.

State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an, China.

Transitory starch is the portion of starch synthesized during the day in the chloroplast and usually used up for plant growth during the night.Here we found altered metabolism of transitory starch in the wxr1/wxr3(weak auxinresponse)mutants of Arabidopsis.WXR1/WXR3 are previously reported to regulate root growth of young seedling and affect auxin response mediated by auxin polar transport in Arabidopsis. In this study thewxr1/wxr3 mutants accumulated transitory starch in the cotyledon, young leaf and hypocotyl at end of night (EON). The expression of WXR1/WXR3follows an obvious circadian rhythm. Grafting experiments proved that the WXRs in root were necessary for proper starch metabolism and plant growth.Other findings include that photosynthesis was inhibited, and the transcription level of DIN1 /DIN6 (Dark-Inducible 1/6) was reducedin wxr1/wxr3. Meanwhile, the mutants showed a defect in ionic equilibrium of Na+ and K+, consistent with our bioinformatics data that genes related to ionic equilibrium were mis-regulated in wxr1. The loss of WXR1 function also resulted in abnormal trafficking of the membrane lipids and proteins. In conclusion, this study reveals that the plastid protein WXR1/WXR3 play important roles in promoting transitory starch degradation for plant growth during the night, possibly through regulating ionic equilibrium in the root.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1093/jxb/erab056DOI Listing
February 2021

Three-year clinical outcomes of a sirolimus-eluting bioresorbable scaffold (XINSORB) and a metallic stent to treat coronary artery stenosis.

Ann Transl Med 2020 Nov;8(22):1489

Shanghai Institute of Cardiovascular Diseases, Department of Cardiology, Zhongshan Hospital, Fudan University, Shanghai, China.

Background: Recent studies have shown increased risks of late target lesion failure (TLF) and thrombosis using a bioresorbable scaffold (BRS). However, the results of the ABSORB China study offered a different means of understanding the long-term performance of BRSs. We tested the 3-year clinical outcome of the XINSORB BRS in a multicenter, randomized controlled clinical trial (ChiCTR1800014966).

Methods: Eligible patients with one or two coronary lesions were randomly assigned 1:1 to be treated with XINSORB scaffolds and metallic sirolimus-eluting stents (SESs). The clinical endpoints include TLF [cardiac death, target vessel-related myocardial infarction (TV-MI), or ischemia-driven target lesion revascularization (ID-TLR)], its components, and devised thrombosis.

Results: Three hundred ninety-five patients were enrolled and randomized to the XINSORB (N=200) and SES (N=195) arms. The clinical 3-year follow-up included 95.5% of the XINSORB-treated patients and 92.8% of the SES-treated patients. Dual antiplatelet therapy was at 59.0% of the XINSORB-treated and 52.8% of the SES-treated patients (P=0.34). There were no significant differences in the clinical outcomes between the XINSORB and SES arms, including in TLF (4.0% 6.2%, P=0.29), cardiac death (1.0% 0%, P=NA), TV-MI (1.0% 0%, P=NA), and ID-TLR (3.5% 6.2%, P=0.19). The rate of confirmed/probable device thrombosis in the XINSORB-treated patients was only 1.0% (2/200).

Conclusions: In this XINSORB randomized clinical trial, the XINSORB scaffolds and SESs showed similar efficacy and safety up to the 3-year follow-up. The rates of TLF and device thrombosis were low and comparable between the two arms.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.21037/atm-20-6739DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7729338PMC
November 2020

Regression analysis of mixed panel count data with informative indicator processes.

Stat Med 2021 Feb 3;40(5):1262-1271. Epub 2020 Dec 3.

Department of Statistics, University of Missouri, Columbia, Missouri, USA.

Panel count data occur often in event history studies and in these situations, one observes only incomplete information, the number of events rather than the occurrence times of each event, about the point processes of interest. Sometimes one may have to face a more complicated type of panel count data, mixed panel count data in which instead of the number of events, one only knows if there is an occurrence of an event. Furthermore, this may depend on the underlying point process of interest or in other words, the point process of interest and the observation type process may be related. To address this, a sieve maximum likelihood estimation approach is proposed with the use of Bernstein polynomials, and for the implementation, an EM algorithm is developed. To assess the finite sample performance of the proposed approach, a simulation study is conducted and suggests that it works well for practical situations. The method is then applied to a motivating example about cancer survivors.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/sim.8839DOI Listing
February 2021

Borneol inhibits CD4 + T cells proliferation by down-regulating miR-26a and miR-142-3p to attenuate asthma.

Int Immunopharmacol 2021 Jan 4;90:107223. Epub 2020 Dec 4.

Department of Pediatrics, The Second Affiliated Hospital of Nanjing Medical University, 262 Zhongshan North Road, Nanjing, Jiangsu 210003, China. Electronic address:

Background: Asthma is a chronic airway inflammatory disease caused by a variety of cytokines and signaling pathways closely related to immunoregulation. Corticosteroids are the most widely used drug in the asthma treatment. However, the use of corticosteroids could cause topical side effects. So, it's important to find new drugs for asthma treatment. Our study aims to explore the pharmacological effect of borneol on asthma and its underlying mechanism.

Methods: We constructed the OVA-induced asthma model to investigate the effect of borneol on asthma in mice. HE and PAS staining was used to detect the effect of borneol on pathological change of mice with asthma. Inflammatory cytokines were measured by ELISA. qRT-PCR was used to explore the effect of borneol on microRNAs expression. Cell proliferation of CD4 + T cells was detected by CCK-8 assay and flow cytometry. Western blot was used to detect pten expression and Akt activation.

Results: We found that borneol significantly alleviated asthma progression in mice. Borneol inhibited CD4 + T cells infiltration in vivo and proliferation in vitro by downregulating miR-26a and miR-142-3p. miR-26a and miR-142-3p promoted CD4 + T cells proliferation in vitro through targeting Pten. Overexpression of miR-26a and miR-142-3p abolished the effect of borneol in vivo.

Conclusion: In a word, these findings suggested that borneol attenuated asthma in mice by decreasing the CD4 + T cells infiltration. The molecular mechanism of borneol was dependent on the downregulation of miR-26a and miR-142-3p to upregulate the Pten expression.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.intimp.2020.107223DOI Listing
January 2021

Final report of the 5-year clinical outcomes of the XINSORB bioresorbable sirolimus-eluting scaffold in the treatment of single coronary lesions in a first-in-human study.

Ann Transl Med 2020 Sep;8(18):1162

Shanghai Institute of Cardiovascular Diseases, Department of Cardiology, Zhongshan Hospital, Fudan University, Shanghai, China.

Background: We aimed to report the 5-year outcomes of XINSORB bioresorbable sirolimus-eluting scaffolds in the treatment of single de novo coronary lesions in a first-in-human (FIM) study. This is the final report of the long-term clinical outcomes of the study. Recent studies have shown that bioresorbable scaffolds (BRSs) increase the risks of late target lesion failure (TLF) and thrombosis.

Methods: In this prospective, single-arm study, eligible patients with single de novo coronary lesions were enrolled and treated with XINSORB scaffolds. The scaffolds measured 3.0 mm in diameter and 12, 15, and 18 mm in length. The clinical endpoints included TLF [cardiac death, target vessel-related myocardial infarction (TV-MI), or ischaemia-driven target lesion revascularization (ID-TLR)], its components, major adverse cardiac events (MACE), and scaffold thrombosis.

Results: From September 2013 to January 2014, 30 patients were enrolled and treated with XINSORB scaffolds. The procedure had a 100% success rate. None of the patients died during the 5 years of follow-up. The primary endpoint of TLF occurred in 4 patients (13.3%). Six patients were recanalized by intervention, including 4 by ID-TLR. The rate of MACE was 16.7% (5/30). One very late case of scaffold thrombosis was recorded, which led to TV-MI. No more cases of thrombosis were recorded beyond 2 years of follow-up. The rates of clinical endpoints remained steady with no changes after 3 years of follow-up.

Conclusions: Considering that this FIM study was launched at an early stage of the BRS era and without optimal implantation techniques, the clinical outcomes of TLF during the 5-year follow-up were acceptable. The rate of thrombosis was relatively low.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.21037/atm-20-5668DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7576036PMC
September 2020

Transcriptome Analyses of β-Thalassemia -28(A>G) Mutation Using Isogenic Cell Models Generated by CRISPR/Cas9 and Asymmetric Single-Stranded Oligodeoxynucleotides (assODNs).

Front Genet 2020 8;11:577053. Epub 2020 Oct 8.

BGI-Shenzhen, Shenzhen, China.

β-thalassemia, caused by mutations in the human hemoglobin β () gene, is one of the most common genetic diseases in the world. The -28(A>G) mutation is one of the five most common mutations in Chinese patients with β-thalassemia. However, few studies have been conducted to understand how this mutation affects the expression of pathogenesis-related genes, including globin genes, due to limited homozygote clinical materials. Therefore, we developed an efficient technique using CRISPR/Cas9 combined with asymmetric single-stranded oligodeoxynucleotides (assODNs) to generate a K562 cell model with -28(A>G) named K562. Then, we systematically analyzed the differences between K562 and K562 at the transcriptome level by high-throughput RNA-seq before and after erythroid differentiation. We found that the -28(A>G) mutation not only disturbed the transcription of , but also decreased the expression of , which may further aggravate the thalassemia phenotype and partially explain the more severe clinical outcome of β-thalassemia patients with the -28(A>G) mutation. Moreover, we found that the K562 cell line is more sensitive to hypoxia and shows a defective erythrogenic program compared with K562 before differentiation. Importantly, all abovementioned abnormalities in K562 were reversed after correction of this mutation with CRISPR/Cas9 and assODNs, confirming the specificity of these phenotypes. Overall, this is the first time to analyze the effects of the -28(A>G) mutation at the whole-transcriptome level based on isogenic cell lines, providing a landscape for further investigation of the mechanism of β-thalassemia with the -28(A>G) mutation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fgene.2020.577053DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580707PMC
October 2020

Wood vinegar enhances humic acid-based remediation material to solidify Pb(II) for metal-contaminated soil.

Environ Sci Pollut Res Int 2021 Mar 21;28(10):12648-12658. Epub 2020 Oct 21.

College of Chemistry and Chemical Engineering, Shaanxi Key Research Laboratory of Chemical Additives, Shaanxi University of Science & Technology, Xi'an, 710021, China.

Heavy metal lead is a typical widespread potentially toxic element (PET) contamination due to their extensive and wide applications in industrial processes. The development of cost-effective methods for preventing potentially toxic element lead residues from soil into food is thus highly desirable. A new type of humic acid-based remediation material (HA/WV) incorporating humic acid salt (HA), biochar powder (BC), and wood vinegar (WV), which is a cheap and environmentally friendly industrial by-product from charcoal processing, was prepared and evaluated. The results showed that 0.10 g remediation material HA/WV with a mass ratio of 1:1 was added to 1 kg surface soil of 0-20 cm from agricultural land contaminated by 300 mg Pb, the reduction ratio of available Pb in soil can reach 61.4%. Especially, wood vinegar can enhance the reduction ratio of available Pb by at least 14.7% over without wood vinegar. Furthermore, according to the analysis of adsorption interaction and the electrostatic attraction between Pb(II) and oxygen-containing functional groups on HA/WV are the dominant mechanisms responsible for Pb(II) sorption. The wood vinegar liquid can improve the oxygen-containing group in HA/WV, which can enhance the complexation of remediation materials and Pb(II) ion.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11356-020-11202-3DOI Listing
March 2021

Laser-induced nano-bismuth decorated CdS-graphene hybrid for plasmon-enhanced photoelectrochemical analysis.

Chem Commun (Camb) 2020 Nov 19;56(89):13784-13787. Epub 2020 Oct 19.

College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University, Qingdao, 266109, People's Republic of China.

Direct-laser-writing of a plasmon-enhanced photoelectrode is successfully demonstrated via the in situ and straightforward formation of a laser-induced Bi-CdS-graphene nanohybrid, which shows a significantly amplified and stable photocurrent response and, thus, further provides a highly sensitive PEC sensing platform.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d0cc05907bDOI Listing
November 2020

Engineering artificial switchable nanochannels for selective monitoring of nitric oxide release from living cells.

Biosens Bioelectron 2020 Dec 10;169:112606. Epub 2020 Sep 10.

Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, College of Chemistry and Chemical Engineering, Hubei University, Wuhan, 430062, PR China. Electronic address:

Nitric oxide (NO) can delicately tune the cellular signaling pathway and plays crucial roles in physiological processes. It is of profound significance to engineer a smart and efficient artificial platform to detect NO, especially for the tracking of living cell released NO. Herein, a switchable nitric oxide responsive nanochannel analysis platform is constructed by introducing a reversible N-nitrosation reaction of rhodamine 6G (R6G) into the artificial nanochannels. By virtue of the distinctive design, ionic current signal can handily realize reversible switching between "on" and "off" state in the presence of NO and UV light, and the system featured high stability and reproducibility. The R6G-immobilized nanochannels exhibited high sensitivity and selectivity towards NO over other gas molecules and biomolecules by ion current rectification (ICR) test. More intriguingly, the system also showed good performances for in situ monitoring of NO released from human umbilical vein endothelial cells (HUVECs), suggesting the as-constructed nanochannels can act as a versatile NO gas valve for nanoelectronic logic devices. This work purposes a novel method for the rapid and noninvasive detection of bioactive gas and holds great promise for biomedical research, disease diagnosis and treatment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bios.2020.112606DOI Listing
December 2020

Nontoxic-dose deoxynivalenol aggravates lipopolysaccharides-induced inflammation and tight junction disorder in IPEC-J2 cells through activation of NF-κB and LC3B.

Food Chem Toxicol 2020 Nov 30;145:111712. Epub 2020 Aug 30.

College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, Jiangsu Province, China; Institute of Nutritional and Metabolic Disorders in Domestic Animals and Fowls, Nanjing Agricultural University, Nanjing, 210095, Jiangsu Province, China; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, Jiangsu Province, China. Electronic address:

Lipopolysaccharide (LPS) is the key factor in various intestinal inflammation which could disrupt the epithelial barrier function. Deoxynivalenol (DON), a well-known mycotoxin, can induce intestinal injury. However, the combined enterotoxicity of LPS and DON has rarely been studied. In this study, IPEC-J2 cell monolayers were exposed to LPS and nontoxic-dose DON for 12 and 24 h to investigate the effects of DON on LPS-induced inflammatory response and tight junction variation, and specific inhibitor and CRISPR-Cas9 were used to explore the underlying mechanisms. Our results showed that nontoxic-dose DON aggravated LPS-induced cellular inflammatory response, reflecting on more significant changes of inflammatory cytokines mRNA expression, higher protein expression of NOD-like receptor protein 3 (NLRP3) and procaspase-1. Moreover, nontoxic-dose DON aggravated LPS-induced mRNA and protein expression decreased, and distribution confused of tight junction proteins. We found that DON further enhanced LPS-induced phosphorylation and nucleus translocation of p65, and expression of LC3B-Ⅱ. NF-κB inhibitor and CRISPR-Cas9-mediated knockout of LC3B attenuated the effects of combination which indicated nontoxic-dose DON aggravated LPS-induced intestinal inflammation and tight junction disorder through activating NF-κB signaling pathway and autophagy-related protein LC3B. It further warns that ingesting low doses of mycotoxins may exacerbate the effects of intestinal pathogens on the body.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.fct.2020.111712DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7456579PMC
November 2020

Network meta-analysis of antiepileptic drugs in focal drug-resistant epilepsy.

Epilepsy Res 2020 11 25;167:106433. Epub 2020 Jul 25.

Department of Radiation Biology, Faculty of Preventive Medicine, The Fourth Military Medical University, Xi'an, China; Ministry of Education Key Lab of Hazard Assessment and Control in Special Operational Environment, Xi'an, China; Shaanxi Key Laboratory of Free Radical Biology and Medicine, China. Electronic address:

Objective: To compare and rank the efficacy and acceptability of new antiepileptic drugs (AEDs) for patients with focal drug-resistant epilepsy.

Methods: PubMed, EMBASE, Cochrane databases and Clinicaltrials.gov were systematically searched from their inception through January 1, 2020, to identify trials evaluating AEDs for focal drug-resistant epilepsy. We included randomized controlled clinical trials (RCTs) comparing new AEDs with placebo or with other AEDs as adjunctive therapy for focal drug-resistant epilepsy. A Bayesian network meta-analysis was performed to determine efficacy and acceptability, as reflected by odds ratios (ORs), 95 % credible intervals (CrIs) with random-effects and consistent models.

Results: Sixty-two RCTs were included, involving 12,739 patients with focal drug-resistant epilepsy. Regarding the seizure-free rate (40 RCTs involving 9,136 patients), 8 AEDs were more efficacious than placebo, with lnORs ranging between 1.69 for brivaracetam (95 % CrI, 0.56-2.81) and 0.72 for pregabalin (95 % CrI, 0.12-1.32). Regarding the responder rate, all AEDs except oxcarbazepine were more efficacious than placebo, with lnORs ranging between 1.31 for levetiracetam (95 % CrI, 0.92-1.71) and 0.66 for carisbamate (95 % CrI, 0.17-1.14). Regarding acceptability (60 RCTs comprising 12,139 patients), 9 AEDs were inferior to placebo. Estimated from seizure-free rate, brivaracetam was ranked as the most efficacious AED based on cumulative probability plots and SUCRAs, with fatigue as the main adverse event.

Conclusion: The results indicate that, based on seizure-free rate and all-cause discontinuation rate, brivaracetam is the most efficacious and acceptable AED, with mild adverse events and acknowledgement of potential publication bias.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.eplepsyres.2020.106433DOI Listing
November 2020

Circulating CD34+VEGFR-2+ endothelial progenitor cells correlate with revascularization-mediated long-term improvement of cardiac function in patients with coronary chronic total occlusions.

Int J Cardiol 2021 Jan 15;322:1-8. Epub 2020 Aug 15.

Department of Cardiology, Zhongshan Hospital, Fudan University, Shanghai Institute of Cardiovascular Diseases, Shanghai, China. Electronic address:

Background: Endothelial progenitor cells (EPCs) participate in angiogenesis and neocollateralization. This study assessed if circulating EPCs can predict long-term improvement of global left ventricular systolic function in patients with coronary chronic total occlusions (CTOs) underwent successful percutaneous coronary intervention (PCI).

Methods: In this single-center, prospective, observational study, 115 consecutive patients with CTOs were evaluated by standard transthoracic echocardiography (ECHO) before and 9-12 months after PCI. Numbers of circulating putative EPCs were determined by flow cytometry analysis of mononuclear cells isolated from peripheral blood samples drawn before and 72 h after PCI.

Results: At mean 11.3 ± 2.5 months post vs. before PCI (all P < .05): by SAQ-7 summary scores, angina frequency, physical limitation and quality of life scores were greater; by ECHO, LVEDd decreased and LVEF increased, which were more significant in patients with Rentrop grades 2/3 vs. 0/1. At 72 h post vs. before PCI, CD34VEGFR-2CD133 (0.82 ± 0.32 × 10/L vs. 1.00 ± 0.39 × 10/L, P = .003), CD34VEGFR-2CD133 (0.24 ± 0.12 × 10/L vs. 0.27 ± 0.14 × 10/L, P = .028), and CD14Tie2VEGFR-2 (6.60 ± 3.32 × 10/L vs. 7.82 ± 3.91 × 10/L, P = .006) cell numbers were lower. The baseline levels of CD34VEGFR-2cells (P = .001) and CD14Tie2VEGFR-2cells (P < .001) were association with the grade of collateralization. In addition, the baseline and peri-procedural decrease of circulating CD34VEGFR-2 cells correlated with the increase of LVEF (P < .001, P < .001, respectively) and the decrease of LVEDd (P = .022, P = .029, respectively) at follow-up.

Conclusions: In this small study, the baseline levels of circulating CD34VEGFR-2+ EPCs and its reduction after successful revascularization of CTOs correlated with long-term improvement in global LV systolic function.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ijcard.2020.08.031DOI Listing
January 2021

Procedural and In-hospital Outcomes of Rotational Atherectomy in Retrograde Coronary Chronic Total Occlusion Intervention.

Angiology 2021 01 17;72(1):44-49. Epub 2020 Aug 17.

Department of Cardiology, 92323Zhongshan Hospital, Fudan University, Shanghai, China.

Coronary chronic total occlusions (CTOs) are characterized by a high incidence of severe plaque calcifications, which are associated with a high use of the retrograde approach and a low success rate of percutaneous coronary intervention (PCI). However, the feasibility of rotational atherectomy (RA) in retrograde CTO-PCI remains unknown. The aim of the present study is to examine the safety and efficacy of RA in retrograde CTO-PCI. Consecutive patients (n = 129) who underwent RA during CTO-PCI were categorized into anterograde and retrograde groups according to the CTO crossing approach. The distributions of the baseline characteristics were similar in the 2 groups, but the lesion type was more complex ( = .001), and the starting burr size was smaller ( = .003) in the retrograde group than in the anterograde group. There was a trend of a higher incidence of procedural complications in the retrograde group than in the anterograde group ( = .054). Technical and procedural success and in-hospital outcomes were not significantly different between the 2 groups. In conclusion, RA was feasible in retrograde CTO PCI, but some specific precautions are required before and during the procedure. In addition, further investigation of the long-term outcomes of RA in retrograde CTO PCI is necessary.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1177/0003319720949312DOI Listing
January 2021

Bi-allelic Loss-of-function Variants in CFAP58 Cause Flagellar Axoneme and Mitochondrial Sheath Defects and Asthenoteratozoospermia in Humans and Mice.

Am J Hum Genet 2020 09 12;107(3):514-526. Epub 2020 Aug 12.

Reproductive Medicine Center, Department of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical University, Hefei 230022, China; NHC Key Laboratory of Study on Abnormal Gametes and Reproductive Tract (Anhui Medical University), Hefei 230032, China; Key Laboratory of Population Health Across Life Cycle (Anhui Medical University), Ministry of Education of the People's Republic of China, Hefei 230032, China. Electronic address:

Multiple morphological abnormalities of the sperm flagella (MMAF) is a severe form of asthenoteratozoospermia. Although recent studies have revealed several MMAF-associated genes and demonstrated MMAF to be a genetically heterogeneous disease, at least one-third of the cases are still not well understood for their etiology. Here, we identified bi-allelic loss-of-function variants in CFAP58 by using whole-exome sequencing in five (5.6%) unrelated individuals from a cohort of 90 MMAF-affected Chinese men. Each of the men harboring bi-allelic CFAP58 variants presented typical MMAF phenotypes. Transmission electron microscopy demonstrated striking flagellar defects with axonemal and mitochondrial sheath malformations. CFAP58 is predominantly expressed in the testis and encodes a cilia- and flagella-associated protein. Immunofluorescence assays showed that CFAP58 localized at the entire flagella of control sperm and predominantly concentrated in the mid-piece. Immunoblotting and immunofluorescence assays showed that the abundances of axoneme ultrastructure markers SPAG6 and SPEF2 and a mitochondrial sheath protein, HSP60, were significantly reduced in the spermatozoa from men harboring bi-allelic CFAP58 variants. We generated Cfap58-knockout mice via CRISPR/Cas9 technology. The male mice were infertile and presented with severe flagellar defects, consistent with the sperm phenotypes in MMAF-affected men. Overall, our findings in humans and mice strongly suggest that CFAP58 plays a vital role in sperm flagellogenesis and demonstrate that bi-allelic loss-of-function variants in CFAP58 can cause axoneme and peri-axoneme malformations leading to male infertility. This study provides crucial insights for understanding and counseling of MMAF-associated asthenoteratozoospermia.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ajhg.2020.07.010DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7477015PMC
September 2020

In situ growth of nano-gold on anodized aluminum oxide with tandem nanozyme activities towards sensitive electrochemical nanochannel sensing.

Analyst 2020 Oct 13;145(20):6617-6624. Epub 2020 Aug 13.

College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University, Qingdao, 266109, People's Republic of China.

Electrochemical nanochannel sensors have attracted extensive interest due to their potential applications in biosensing systems. In this work, porous anodized aluminum oxide (AAO) nanochannels are coupled with gold nanoparticles (AuNPs) through a polydopamine (PDA)-induced in situ growth process. It is found that the resulting hybrid nanochannel (denoted as Au-PDA-AAO) can act as both glucose oxidase- and peroxidase-like nanozymes to catalyze the cascade reaction involving glucose. To the best of our knowledge, this is the first report on the synthesis of nanozymes in an AAO nanochannel. Moreover, apart from the nanozyme-catalyzed colorimetric reaction, the Au-PDA-AAO nanochannel could simultaneously serve as a sensitive signal reporter for an electrochemical sensing platform. In such an approach, the glucose oxidation reaction boosts the resistance of the Au-PDA-AAO nanochannel towards ion transport based on the HO-mediated size enlargement of AuNPs, resulting in the varied transmembrane ionic current signal of the Au-PDA-AAO nanochannel. On the basis of the changed current-potential properties, the label-free detection of glucose can be achieved with a low detection limit, good reproducibility, and high stability. This work demonstrates the feasibility of the incorporation of versatile nanozymes into AAO nanochannels for mimicking multi-enzymatic catalysis reactions and detecting target analytes.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d0an01271hDOI Listing
October 2020

Identification of CD38 as a potential biomarker in skin cutaneous melanoma using bioinformatics analysis.

Oncol Lett 2020 Oct 15;20(4):12. Epub 2020 Jul 15.

Department of Sports Medicine, School of Education and Physical Education, Yangtze University, Jingzhou, Hubei 434023, P.R. China.

Skin cutaneous melanoma (SKCM) is the most aggressive type of skin cancer, with a high rate of metastasis and mortality; however, identification of biomarkers for the treatment of SKCM is required. Cluster of differentiation (CD)38 has emerged as an effective target for therapeutic drugs in several types of cancer, such as chronic lymphocytic leukemia and multiple myeloma. In the present study, to determine the contribution of CD38 to the diagnosis of SKCM, Gene Expression Profiling Interactive Analysis 2 and University of Alabama Cancer Database online tools were used to analyze The Cancer Genome Atlas-SKCM dataset. Moreover, Search Tool for the Retrieval of Interacting Genes/Proteins and GeneMANIA databases were used to determine protein-protein interaction networks and potential functions. To the best of our knowledge, the results of the present study indicated for the first time that high expression levels of CD38 were a favorable diagnostic factor for SKCM. Moreover, a correlation between CD38 expression levels and the survival probability of patients with SKCM was identified. Integrative analysis predicted that nine genes were correlated with CD38 in SKCM, and the similarity of these genes in SKCM expression and a survival heatmap was verified. Gene ontology enrichment analysis using the Metascape tool revealed that CD38 and its correlated genes were significantly enriched in lymphocyte activation and T cell differentiation regulation. Collectively, the bioinformatics analysis revealed that CD38 might serve as a potential diagnostic predictor for SKCM.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3892/ol.2020.11873DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7405635PMC
October 2020

Vitamin D Attenuates Hypoxia-Induced Injury in Rat Primary Neuron Cells through Downregulation of the Dual Oxidase 1 (DUOX1) Gene.

Med Sci Monit 2020 Jul 26;26:e925350. Epub 2020 Jul 26.

Department of Emergency, People's Hospital of Rizhao, Rizhao, Shandong, China (mainland).

BACKGROUND This study aimed to investigate the mechanisms underlying the neuroprotective effects of vitamin D. MATERIAL AND METHODS Rat primary neuron cells were incubated under a hypoxia condition [a hypoxic chamber mixed with anaerobic gas (90% N₂, 5% CO₂) and 5% O₂] to induce cell injury. Cell transfection was performed to overexpress or suppress the expression of dual oxidase 1 (DUOX1). The malondialdehyde (MDA) and superoxide dismutase (SOD) levels were detected using a MDA (A003-2) or SOD (A001-1) kit. DUOX1 mRNA levels were detected using RT-PCR. Hypoxia-inducible factor-1alpha (HIF-1alpha), DUOX1, vitamin D receptor (VDR), NF-kappaB protein expressions were determined by western blotting. Cell apoptosis and reactive oxygen species (ROS) were evaluated by flow cytometry. RESULTS ROS increased significantly after hypoxic treatment. The expressions of HIF-1alpha and DUOX1 were significantly increased after hypoxic treatment. Vitamin D could decrease ROS level, apoptotic neuron cells and DUOX1 expression, and increase VDR expression. Downregulation of DUOX1 significantly decreased MDA level and apoptotic percentages of neuron cells, increased SOD level, and counteracted the hypoxia-induced increase of NF-kappaB signal. Further study showed that overexpression of DUOX1 significantly increased MDA level, ROS level, apoptotic percentages of neuron cells, and NF-kappaB nuclear signaling, while decreased SOD level. Vitamin D significantly counteracted the effects of DUOX1 overexpression induced injury in rat primary neuron cells. CONCLUSIONS Our study indicated that vitamin D may protect neuron cells from hypoxia-induced injury by regulating DUOX1 via the NF-kappaB signaling pathway.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.12659/MSM.925350DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7405618PMC
July 2020

The relationship between bacterial diversity and organic carbon mineralization in soft rock and sand compound soil.

J Microbiol 2020 Sep 24;58(9):750-760. Epub 2020 Jul 24.

Shaanxi Provincial Land Engineering Construction Group Co., Ltd., Xi'an, 710075, P. R. China.

The soil organic carbon (SOC) mineralization rate in sandy soil plays an important role in improving soil quality, and a research is needed to determine management practices that optimize the mineralization rate. When sandy soil is improved by adding soft rock, the specific promotion process of bacterium to SOC mineralization remain unclear. To investigate these mechanisms, we selected four treatments with soft rock to sand volume ratios of 0:1 (CK), 1:5 (C1), 1:2 (C2) and 1:1 (C3) to study. The mineralization rate of organic carbon was measured using the lye absorption method. High-throughput sequencing and scanning electron microscopy were used to determine the bacterial community structure and soil microstructure, respectively. The results showed that the organic carbon content of the sandy soil increased significantly (182.22-276.43%) after using the soft rock treatments. The SOC mineralization rate could be divided into two stages: a rapid decline during days 1-8 and a slow decline during days 8-60. With increased incubation time, the intensity of the cumulative release of organic carbon gradually weakened. Compared with the CK treatment, the SOC mineralization accumulation (Ct) and the potential mineralizable organic carbon content (C) in the C1, C2, and C3 treatments increased significantly, by 106.98-225.94% and 112.22-254.08%, respectively. The cumulative mineralization rate (Cr) was 18.11% and 21.38% smaller with treatments C2 and C3, respectively. The SOC mineralization rate constant (k) decreased significantly after the addition of soft rock, while the half-turnover period (Th) changed inversely with k. Compared with the CK treatment, the number of gene copies of the soil bacteria increased by 15.38-272.53% after adding soft rock, with the most significant increase in treatment C3. The bacterial diversity index also increased significantly under treatment C3. The three dominant bacteria were Proteobacteria, Actinobacteria, and Chloroflexi. The correlation between C and one of the non-dominant bacteria, Firmicutes, was large, and the bacteria had a significant positive correlation with k. At the same time, the abundance of Firmicutes under treatments C2 and C3 was small. As the proportion of soft rock increased, the soil particles changed from point contact to surface contact, and the adhesion on the surface of the particles gradually increased. Results from this study show that the retention time of SOC can be increased and the carbon sequestration effect is better when the ratio of soft rock to sand is set to 1:2.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12275-020-0130-4DOI Listing
September 2020

Low-level contamination of deoxynivalenol: A threat from environmental toxins to porcine epidemic diarrhea virus infection.

Environ Int 2020 10 13;143:105949. Epub 2020 Jul 13.

College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, China; Institute of Nutritional and Metabolic Disorders in Domestic Animals and Fowls, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, China; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, China. Electronic address:

Mycotoxins are toxic metabolites produced by fungal species that commonly present in the global environment, especially in cereals and animal forages. The changing global environment may further increase the exposure to these toxins, posing a serious threat to humans and animals. Recently, coronavirus has become one of the most important pathogens threatening human and animal health. It is not clear whether environmental toxins, such as mycotoxins, will affect coronavirus infection. Given that pigs are among the animals most affected by coronavirus and highly homologous to humans, weaned piglets and IPEC-J2 cells were respectively chosen as in vivo and in vitro model to explore the impacts of deoxynivalenol (DON), the most abundant trichothecene mycotoxin in feed, on porcine epidemic diarrhea virus (PEDV) infection and the mechanisms involved. In vivo, twenty-seven piglets infected naturally with PEDV were randomly divided into three groups, receiving the basal diet containing 0, 750 and 1500 μg/kg DON, respectively. Significant increases in the diarrhea rates, gut barrier injury and PEDV proliferation of piglets' small intestine were observed in experimental groups compared with the control. Additionally, the autophagosome-like vesicles and the autophagy-related proteins expression were also increased in experimental groups. In vitro, we observed that 0.1, 0.5 and 1.0 μM DON significantly promoted the entry and replication of PEDV in IPEC-J2 cells, along with the induction of a complete autophagy. CRISPR-Cas9-mediated knockout of LC3B indicated a vital role of autophagy in the promotion. Pretreatment with p38 signaling inhibitor could significantly block the induction of autophagy, indicating that DON could promote the PEDV infection by triggering p38-mediated autophagy. Our findings suggest that mycotoxin could influence the prevalence of coronavirus and provide new ideas for the prevention and control of coronavirus.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.envint.2020.105949DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7357974PMC
October 2020

Ochratoxin A induces glomerular injury through activating the ERK/NF-κB signaling pathway.

Food Chem Toxicol 2020 Sep 30;143:111516. Epub 2020 Jun 30.

College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, Jiangsu Province, China; Institute of Nutritional and Metabolic Disorders in Domestic Animals and Fowls, Nanjing Agricultural University, Nanjing, 210095, Jiangsu Province, China; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, Jiangsu Province, China. Electronic address:

Ochratoxin A (OTA) was reported to induce proximal tubules nephrotoxicity in humans and animals. However, the toxicity of OTA on glomeruli has rarely been studied. We investigated OTA-induced glomerular injury and the underlying mechanisms. Mice were intraperitoneally treated with OTA (0, 0.5, 1.5 and 2.5 mg/kg b.w.) on alternate day for 3 weeks. OTA exposure decreased the weight gain ratio, the kidney index and increased the levels of serum creatinine and blood urea nitrogen. It induced also fragmentation and atrophy in glomeruli, and increased the expression of TNF-α, IL-6, COX-2, TGF-β, α-SMA and vimentin in a dose-dependent manner. Human mesangial cells (HMC) were treated with OTA (0-8 μM) for 48 h. Treatment of HMC cells with OTA increased cell inhibition rate, up-regulated the expression of IL-6, TGF-β, α-SMA and vimentin in a dose-dependent manner. Additionally, it enhanced the phosphorylation of ERK1/2 and p65, degradation of IκB-α and translocation of p65 into the nucleus. OTA-induced toxicity was attenuated by NF-κB and ERK1/2 inhibitors. In conclusion, these results suggest that OTA exposure induces glomerular injury via activation of the ERK/NF-κB signaling pathway, and provide novel insights into the research of OTA induced nephrotoxicity.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.fct.2020.111516DOI Listing
September 2020

Rational Prediction of Single Metal Atom Supported on Two-Dimensional Metal Diborides for Electrocatalytic N Reduction Reaction with Integrated Descriptor.

J Phys Chem Lett 2020 Jul 18;11(13):5241-5247. Epub 2020 Jun 18.

Department of Chemistry, Institute for Functional Nanomaterials, University of Puerto Rico, Rio Piedras Campus, San Juan, Puerto Rico 00931, United States.

Nitrogen reduction reaction (NRR) plays an important role in chemical industry, so it is significant to develop low-cost and efficient electrocatalysts for nitrogen fixation instead of the traditional Haber-Bosch process. In this paper, the electrocatalytic performance of various single atoms doped on two-dimensional metal diborides with a B vacancy for N reduction to ammonia is calculated and predicted. By screening numerous catalysts, we find that Ti@VB is the most active catalyst for NRR, and the limiting potential of Ti@VB for NRR is -0.61 V. Through high-throughput search and LASSO regression, an integrated descriptor combining the intrinsic properties of the single transition metal atom (TM) and the substrate (MB) is proposed, which can fit the relationship between intrinsic properties of catalysts and NRR activity well. Therefore, this study not only discovers a promising electrocatalyst for nitrogen fixation but also provides a strategy for predicting the activity of catalysts.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.jpclett.0c01582DOI Listing
July 2020

Oral administration of olaquindox negatively affects oocytes quality and reproductive ability in female mice.

Ecotoxicol Environ Saf 2020 Sep 7;201:110826. Epub 2020 Jun 7.

State Key Laboratory of Reproductive Regulation & Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot, People's Republic of China. Electronic address:

As an effective feed additive in the livestock industry, olaquindox (OLA) has been widely used in domestic animal production. However, it is unclear whether OLA has negative effects on mammalian oocyte quality and fetal development. In this study, toxic effects of OLA were tested by intragastric gavage ICR mice with water, low-dose OLA (5 mg/kg/day), or high-dose OLA (60 mg/kg/day) for continuous 45 days. Results showed that high-dose OLA gavage severely affected the offspring birth and growth. Significantly, high-dose OLA impaired oocyte maturation and early embryo development, indicated by the decreased percentage of germinal vesicle breakdown, first polar body extrusion and blastocyst formation. Meanwhile, oxidative stress levels were increased in oocytes or ovaries, indexed by the increased levels of ROS, MDA, HO, NO, and decreased levels of GSH, SOD, CAT, GSH-Px and GSH-Rd. Furthermore, aberrant mitochondria distribution, defective spindle assembly, abnormal H3K4me2/H3K9me3 levels, increased DNA double-strand breaks and early apoptosis rate, were observed after high-dose OLA gavage. Taken together, our results for the first time illustrated that high-dose OLA gavage led to sub-fertility of females, which means that restricted utilization of OLA as feed additive should be considered.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ecoenv.2020.110826DOI Listing
September 2020

Laser-induced graphene hybrid photoelectrode for enhanced photoelectrochemical detection of glucose.

Analyst 2020 Jun 5;145(11):4041-4049. Epub 2020 May 5.

College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University, Qingdao, 266109, People's Republic of China.

The combination of an electrocatalyst with a semiconductor light absorber is of great importance to increase the efficiency of photoelectrochemical (PEC) glucose detection. Here, in situ and synchronous fabrication of a Ni-based electrocatalyst (NiEC) and CdS semiconductor in laser-induced graphene (LIG) on indium-tin oxide glass is demonstrated via a one-step laser-induced solid phase transition. A series of component and structural characterization experiments suggest that the laser-induced NiEC uniformly disperses in the hybrid nanocomposite and exists mainly in the Ni and NiO states. Moreover, both electrochemical and PEC investigations confirm that the as-prepared hybrid photoelectrode exhibits excellent photoelectrocatalytic ability towards glucose, which is not only attributed to the strong synergistic interaction between CdS and NiEC, but also benefited from the high conductivity as well as 3D macroporous configuration of the simultaneously formed LIG, providing the key factor to achieve sensitive non-enzymatic PEC glucose sensors. Therefore, the laser-induced hybrid photoelectrode is then applied to the PEC detection of glucose, and a low detection limit of 0.4 μM is obtained with good stability, reproducibility, and selectivity. This study provides a promising paradigm for the facile and binder-free fabrication of an electrocatalyst-semiconductor-graphene hybrid photoelectrode, which will find potential applications in sensitive PEC biosensing for a broad range of analytes.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d0an00252fDOI Listing
June 2020

The preservation effect of coronary collateral circulation on left ventricular function in chronic total occlusion and its association with the expression of vascular endothelial growth factor A.

Adv Clin Exp Med 2020 Apr;29(4):493-497

Department of Cardiology, Fudan University Affiliated Zhongshan Hospital, Shanghai, China.

Background: Patients with coronary chronic total occlusion (CTO) typically have collateralization of the distal vessel, and these collaterals can contribute to the relief of ischemia and anginal symptoms and to the preservation of ventricular function.

Objectives: To investigate the preservation effect of coronary collateral circulation on left ventricular (LV) function in coronary CTO, and to explore the potential mechanism behind the development of coronary collateral circulation.

Material And Methods: A total of 102 consecutive patients with coronary CTO were divided into 2 groups: the left ventricular ejection fraction (LVEF)-preserved group (LVEF ≥ 50%; n = 46) and the LVEF-decreased group (LVEF < 50%; n = 56). Clinical, angiographic and laboratory data was collected for all patients. The association between LVEF and coronary collateral circulation in coronary CTO patients was analyzed with multivariate logistic regression analysis, and the serum levels of VEGF-A and the mRNA expression levels of the VEGF-A gene were compared between different grades of coronary collateral circulation.

Results: Multivariate analysis revealed that Rentrop grades 2-3 and coexisting collateral pathways were independent predictors of LVEF preservation in coronary CTO patients. Patients with Rentrop grades 2-3 had smaller left ventricular end diastolic diameters (LVDd) and left ventricular end systolic diameters (LVSd), and they had larger LVEFs than the patients with Rentrop grades 0-1. Patients with Rentrop grades 2-3 also had higher serum levels of VEGF-A and higher mRNA expression levels of the VEGF-A gene in their peripheral blood mononuclear cells (PBMCs) than patients with Rentrop grades 0-1. Patients with coexisting collateral pathways had higher serum levels of VEGF-A and higher mRNA expression levels of the VEGF-A gene in PBMCs than patients without coexisting collateral pathways.

Conclusions: Coronary collateral circulation is significantly associated with LVEF preservation, and VEGF-A might promote the formation of coronary collateral circulation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.17219/acem/104535DOI Listing
April 2020

Modulated Sn Oxidation States over a CuO-Derived Substrate for Selective Electrochemical CO Reduction.

ACS Appl Mater Interfaces 2020 May 7;12(20):22760-22770. Epub 2020 May 7.

School of Chemical Engineering, The University of Queensland, St Lucia, 4072 Brisbane, Queensland, Australia.

Pursuing high catalytic selectivity is challenging but paramount for an efficient and low-cost CO electrochemical reduction (COR). In this work, we demonstrate a significant correlation between the selectivity of COR to formate and the duration of tin (Sn) electrodeposition over a cuprous oxide (CuO)-derived substrate. A Sn electrodeposition time of 120 s led to a cathode with a formate Faradaic efficiency of around 81% at -1.1 V vs reversible hydrogen electrode (RHE), which was more than 37% higher than those of the Sn foil and the sample treated for 684 s. This result highlights the significant role of the interface between deposited Sn and the cuprous-derived substrate in determining the selectivity of COR. High-resolution X-ray photoelectron spectra revealed that the residual cuprous species at the Cu/Sn interfaces could stabilize Sn species in oxidation states of 2+ and 4+, a mixture of which is essential for a selective formate conversion. Such modulation effects likely arise from the moderate electronegativity of the cuprous species that is lower than that of Sn but higher than that of Sn. Our work highlights the significant role of the substrate in the selectivity of the deposited catalyst and provides a new avenue to advance selective electrodes for CO electrochemical reduction.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.0c00412DOI Listing
May 2020

Electrochemical Reduction of CO to Ethane through Stabilization of an Ethoxy Intermediate.

Angew Chem Int Ed Engl 2020 Oct 25;59(44):19649-19653. Epub 2020 May 25.

School of Chemical Engineering and Advanced Materials, The University of Adelaide, Adelaide, SA, 5005, Australia.

Electrochemical conversion of CO into ethane is seldom observed because of the generally higher selectivity towards methane, ethylene, and ethanol. Consequently, little experimental evidence for its reaction mechanism exists and thus remains largely unknown. Now, by combining electrochemistry with in situ X-ray absorption fine-structure and in situ Raman techniques, iodide-derived copper (ID-Cu) and oxide-derived copper (OD-Cu) systems were studied to obtain a deeper understanding of the CO to ethane mechanism. With trace iodine species on the surface and positively charged Cu species, production of ethane is significantly more favored on ID-Cu compared to OD-Cu, with higher selectivity and faster kinetics. For the first time, it is experimentally found that the formation of ethane follows the same pathway to ethylene and ethanol, and better stabilization of the late stage ethoxy intermediate can steer the reaction to ethane over ethanol.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/anie.202004846DOI Listing
October 2020

Direct evidence of boosted oxygen evolution over perovskite by enhanced lattice oxygen participation.

Nat Commun 2020 Apr 24;11(1):2002. Epub 2020 Apr 24.

WA School of Mines: Minerals, Energy and Chemical Engineering (WASM-MECE), Curtin University, Perth, WA, 6102, Australia.

The development of oxygen evolution reaction (OER) electrocatalysts remains a major challenge that requires significant advances in both mechanistic understanding and material design. Recent studies show that oxygen from the perovskite oxide lattice could participate in the OER via a lattice oxygen-mediated mechanism, providing possibilities for the development of alternative electrocatalysts that could overcome the scaling relations-induced limitations found in conventional catalysts utilizing the adsorbate evolution mechanism. Here we distinguish the extent to which the participation of lattice oxygen can contribute to the OER through the rational design of a model system of silicon-incorporated strontium cobaltite perovskite electrocatalysts with similar surface transition metal properties yet different oxygen diffusion rates. The as-derived silicon-incorporated perovskite exhibits a 12.8-fold increase in oxygen diffusivity, which matches well with the 10-fold improvement of intrinsic OER activity, suggesting that the observed activity increase is dominantly a result of the enhanced lattice oxygen participation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41467-020-15873-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7181763PMC
April 2020