Publications by authors named "Lei Du"

573 Publications

Graph Convolutional Networks Reveal Network-Level Functional Dysconnectivity in Schizophrenia.

Schizophr Bull 2022 May 15. Epub 2022 May 15.

Department of Psychosis Studies, Institute of Psychiatry, Psychology & Neuroscience, King's College London, London, UK.

Background And Hypothesis: Schizophrenia is increasingly understood as a disorder of brain dysconnectivity. Recently, graph-based approaches such as graph convolutional network (GCN) have been leveraged to explore complex pairwise similarities in imaging features among brain regions, which can reveal abstract and complex relationships within brain networks.

Study Design: We used GCN to investigate topological abnormalities of functional brain networks in schizophrenia. Resting-state functional magnetic resonance imaging data were acquired from 505 individuals with schizophrenia and 907 controls across 6 sites. Whole-brain functional connectivity matrix was extracted for each individual. We examined the performance of GCN relative to support vector machine (SVM), extracted the most salient regions contributing to both classification models, investigated the topological profiles of identified salient regions, and explored correlation between nodal topological properties of each salient region and severity of symptom.

Study Results: GCN enabled nominally higher classification accuracy (85.8%) compared with SVM (80.9%). Based on the saliency map, the most discriminative brain regions were located in a distributed network including striatal areas (ie, putamen, pallidum, and caudate) and the amygdala. Significant differences in the nodal efficiency of bilateral putamen and pallidum between patients and controls and its correlations with negative symptoms were detected in post hoc analysis.

Conclusions: The present study demonstrates that GCN allows classification of schizophrenia at the individual level with high accuracy, indicating a promising direction for detection of individual patients with schizophrenia. Functional topological deficits of striatal areas may represent a focal neural deficit of negative symptomatology in schizophrenia.
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http://dx.doi.org/10.1093/schbul/sbac047DOI Listing
May 2022

LGR4 cooperates with PrPc to endow the stemness of colorectal cancer stem cells contributing to tumorigenesis and liver metastasis.

Cancer Lett 2022 May 10:215725. Epub 2022 May 10.

The State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences. Beijing, 100101, China; Beijing Institute for Stem Cell and Regenerative Medicine. Beijing, 100101, China. Electronic address:

Cancer stem cells (CSCs) are a subpopulation of cancer cells that drive tumour progression and metastasis. Anti-CSC strategies represent new targets for cancer therapies. However, CSCs are highly plastic and heterogeneous, making validation and targeting difficult without bona fide markers that define their identity, especially in a clinical setting. Here, we report that a leucine-rich repeat containing G protein-coupled receptor 4 (LGR4) cooperates with CD44 and PrPc; the latter contributes significantly to metastatic capacity and defines the stemness characteristics of colorectal CSCs. CD44PrPcLGR4 cells effectively developed into organoids and, when transplanted, generated orthotopic and metastatic tumours. Importantly, targeting LGR4 and PrPc with lentiviral shRNAs inhibited organoid development and the growth of orthotopic tumours by inhibiting Wnt/β-catenin signalling. Thus, our study offers a novel therapeutic strategy that simultaneously targets CSC stemness and metastatic properties.
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http://dx.doi.org/10.1016/j.canlet.2022.215725DOI Listing
May 2022

The complete chloroplast genome of , a medicinal plant.

Mitochondrial DNA B Resour 2022 9;7(5):796-797. Epub 2022 May 9.

Traditional Chinese Medicine Processing Technology Innovation Center of Hebei Province, Hebei University of Chinese Medicine, Shijiazhuang, China.

(Hooker) Sweet is a semi deciduous or deciduous shrub belonging to the genus Leguminosae. has been used as a folk medicinal herb in Nepal and was cultivated all over the world as an ornamental plant. In the present study, we sequenced the entire genome of the chloroplast of . The total length of the chloroplast genome in is 152,195 bp, including a large single-copy region of 82,048 bp, a small single-copy region of 17,675 bp, and a pair of inverted repeats regions of 26,236 bp. The overall guanine-cytosine (GC) content of the genome was 36.7%. There are 131 genes in the chloroplast genome of , including 85 protein-coding genes, 8 rRNA genes and 38 tRNA genes. Phylogenetic analysis showed that is closely related to .
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http://dx.doi.org/10.1080/23802359.2021.1994897DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9090406PMC
May 2022

Topologically Disrupted Gray Matter Networks in Drug-Naïve Essential Tremor Patients With Poor Sleep Quality.

Front Neurol 2022 26;13:834277. Epub 2022 Apr 26.

Department of Neurology, West China Hospital, Sichuan University, Chengdu, China.

Background: Sleep disturbances are widespread among patients with essential tremor (ET) and may have adverse effects on patients' quality of life. However, the pathophysiology underlying poor quality of sleep (QoS) in patients with ET remains unclear. Our study aimed to identify gray matter (GM) network alterations in the topological properties of structural MRI related to QoS in patients with ET.

Method: We enrolled 45 ET patients with poor QoS (SleET), 59 ET patients with normal QoS (NorET), and 66 healthy controls (HC), and they all underwent a three-dimensional T1-weighted MRI scan. We used a graph-theoretical approach to investigate the topological organization of GM morphological networks, and individual morphological brain networks were constructed according to the interregional similarity of GM volume distributions. Furthermore, we performed network-based statistics, and partial correlation analyses between topographic features and clinical characteristics were conducted.

Results: Global network organization was disrupted in patients with ET. Compared with the NorET group, the SleET group exhibited disrupted topological GM network organization with a shift toward randomization. Moreover, they showed altered nodal centralities in mainly the frontal, temporal, parietal, and cerebellar lobes. Morphological connection alterations within the default mode network (DMN), salience, and basal ganglia networks were observed in the SleET group and were generally more extensive than those in the NorET and HC groups. Alterations within the cerebello-thalamo-(cortical) network were only detected in the SleET group. The nodal degree of the left thalamus was negatively correlated with the Fahn-Tolosa-Marin Tremor Rating Scale score ( = -0.354, =0.027).

Conclusion: Our findings suggest that potential complex interactions underlie tremor and sleep disruptions in patients with ET. Disruptions within the DMN and the cerebello-thalamo-(cortical) network may have a broader impact on sleep quality in patients with ET. Our results offer valuable insight into the neural mechanisms underlying poor QoS in patients with ET.
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http://dx.doi.org/10.3389/fneur.2022.834277DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9086904PMC
April 2022

Perfusion of brain and viscera using modified retrograde cerebral perfusion for aortic arch surgery.

Perfusion 2022 May 11:2676591221092154. Epub 2022 May 11.

Department of Anesthesiology, West China Hospital, Sichuan University, Chengdu, China.

Anterograde or retrograde cerebral perfusion can protect the brain from ischemic injury during hypothermic circulatory arrest (HCA), but neither type of perfusion provides blood flow to the abdominal viscera. Here, we report a modified retrograde cerebral perfusion (RCP) technique in which we tethered both superior and inferior venae cavae with bands around the cannula and clamped the distal ends of the drainage tubes of both venae cavae. Modified RCP may provide greater blood flow to the brain and lower body than conventional RCP during HCA in hemiarch surgery.
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http://dx.doi.org/10.1177/02676591221092154DOI Listing
May 2022

Magnetization transfer imaging alterations and its diagnostic value in antipsychotic-naïve first-episode schizophrenia.

Transl Psychiatry 2022 May 6;12(1):189. Epub 2022 May 6.

Huaxi MR Research Center (HMRRC), Department of Radiology, West China Hospital of Sichuan University, Chengdu, 610041, Sichuan, China.

Magnetization transfer imaging (MTI) may provide more sensitivity and mechanistic understanding of neuropathological changes associated with schizophrenia than volumetric MRI. This study aims to identify brain magnetization transfer ratio (MTR) changes in antipsychotic-naïve first-episode schizophrenia (FES), and to correlate MTR findings with clinical symptom severity. A total of 143 individuals with antipsychotic-naïve FES and 147 healthy controls (HCs) were included and underwent 3.0 T brain MTI between August 2005 and July 2014. Voxelwise analysis was performed to test for MTR differences with family-wise error corrections. Relationships of these differences to symptom severity were assessed using partial correlations. Exploratory analyses using a support vector machine (SVM) classifier were conducted to discriminate FES from HCs using MTR maps. Model performance was examined using a 10-fold stratified cross-validation. Compared with HCs, individuals with FES exhibited higher MTR values in left thalamus, precuneus, cuneus, and paracentral lobule, that were positively correlated with schizophrenia symptom severity [precuneus (r = 0.34, P = 0.0004), cuneus (r = 0.33, P = 0.0006) and paracentral lobule (r = 0.37, P = 0.001)]. Whole-brain MTR maps identified individuals with FES with overall accuracy 75.5% (219 of 290 individuals) based on SVM approach. In antipsychotic-naïve FES, clinically relevant biophysical abnormalities detected by MTI mainly in the left parieto-occipital regions are informative about local brain pathology, and have potential as diagnostic markers.
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http://dx.doi.org/10.1038/s41398-022-01939-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9076920PMC
May 2022

Efficacy and safety of phacoemulsification cataract surgery with prophylactic vitreous injection of bevacizumab in the treatment of retinopathy: a systematic review and meta-analysis.

Ann Palliat Med 2022 Apr;11(4):1421-1430

Department of Ophthalmology, Renmin Hospital of Wuhan University, Wuhan, China.

Background: For patients with cataracts and retinopathy, phacoemulsification can improve the progress of the disease to a certain extent. However, the efficacy of phacoemulsification alone is often not ideal, and may even aggravate the prognostic effects, such as macular edema and slow visual recovery. Thus, an increasing number of clinicians have explored comprehensive therapy involving phacoemulsification combined with drug therapy, which has led to some research progress. However, there is still great controversy about the combined treatment. In this study, we performed a literature meta-analysis to systematically evaluate the clinical efficacy and safety of prophylactic intravitreal injection of bevacizumab combined with phacoemulsification for the treatment of cataracts complicated with retinopathy.

Methods: We searched the PubMed, Web of Science, Embase, and MEDLINE databases for English language papers related to these studies factors published between January 2001 and January 2021. The retrieved articles were screened and the quality of the included studies was evaluated. Meta-analysis was performed and RevMan5.3 was used to calculate the enhanced mean difference (MD).

Results: Six articles were finally included in this meta-analysis, involving a total of 325 cases with cataracts and retinopathy. The results showed that the best-corrected visual acuity at 1 month [MD =-0.06; 95% confidence interval (CI): (-0.09, -0.03); P=0.0002] and 3 months [MD =-0.09; 95% CI: (-0.11, -0.07); P<0.00001] in the preventive vitreous injection bevacizumab intervention group were significantly better than those of the control group (P<0.1). However, the best-corrected visual acuity at 6 months [MD =-0.02; 95% CI: (-0.07, 0.03); P=0.39] was not significantly different between these two groups (P>0.05). In addition, the central macular thickness of the preventive vitreous injection bevacizumab intervention group at 1 month after treatment [MD =-37.07; 95% CI: (-45.87, -28.27); P<0.00001], 3 months [MD =-15.26; 95% CI: (-23.87, -6.66); P=0.0005], and 6 months [MD =-26.77; 95% CI: (-37.51, -16.04); P<0.00001] were significantly different to the control group (P<0.05).

Discussion: The results of this study showed that prophylactic intravitreal injection of bevacizumab combined with phacoemulsification is a safe and effective treatment for patients with cataracts and retinopathy within 6 months after treatment.
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http://dx.doi.org/10.21037/apm-22-222DOI Listing
April 2022

Environmental regulation and green energy efficiency: an analysis of spatial Durbin model from 30 provinces in China.

Environ Sci Pollut Res Int 2022 May 5. Epub 2022 May 5.

School of Economics and Management, Yanshan University, Qinhuangdao, Hebei, 066004, People's Republic of China.

Improving green energy efficiency is crucial to promoting China's high-quality economic development and reducing the environmental pollution. In this paper, the Malmquist-Luenberger index measures the green energy efficiency of 30 provinces in China from 2004 to 2019. Based on the measurement results, the spatial Durbin model is used to empirically study the impact of environmental regulation on green energy efficiency and its spatial spillover effects. The results show that China's green energy efficiency is low, and its growth mainly comes from technological progress (TECH) rather than technological efficiency (EFFCH). The eastern region has the highest efficiency of green energy, followed by the West and the lowest in the Central region. The estimation results of the spatial Durbin model show that both environmental regulation and green energy efficiency have a significant spatial correlation. Environmental regulation can improve the green energy efficiency in the local province but inhibit green energy efficiency in the adjacent provinces. Finally, this paper puts forward relevant policy suggestions according to the research results.
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http://dx.doi.org/10.1007/s11356-022-20551-0DOI Listing
May 2022

Cortical remodeling before and after successful temporal lobe epilepsy surgery.

Acta Neurol Scand 2022 May 4. Epub 2022 May 4.

Department of Neurology, West China Hospital, Sichuan University, Chengdu, China.

Objectives: To explore dynamic alterations of cortical thickness before and after successful anterior temporal lobectomy (ATL) in patients with unilateral mesial temporal lobe epilepsy (mTLE).

Materials And Methods: High-resolution T1-weighted MRI was obtained in 28 mTLE patients who achieved seizure freedom for at least 24 months after ATL and 29 healthy controls. Patients were scanned at five timepoints, including before surgery, 3, 6, 12 and 24 months after surgery. Preoperative cortical thickness of mTLE patients were compared with healthy controls. Dynamic alterations of cortical thickness before and after surgery were compared among five scans using linear mixed models.

Results: Patients with mTLE showed cortical thinning pre-surgically in ipsilateral entorhinal cortex, parahippocampal gyrus, inferior parietal cortex, lateral occipital cortex; contralateral pericalcarine cortex (PCC); and bilateral caudal middle frontal gyrus (cMFG), paracentral lobule, precentral gyrus (PCG), superior parietal cortex. Cortical thickening was observed in contralateral rostral anterior cingulate cortex (rACC). Patients showed postsurgical cortical thinning in ipsilateral temporal lobe, fusiform gyrus, caudal anterior cingulate cortex, lingual gyrus, and insula. Ipsilateral cMFG, PCC, and contralateral PCG showed significant cortical thickening after surgery. In addition, contralateral rACC showed cortical thickening at 3 months follow-up, however, with obvious cortical thinning at 24 months follow-up.

Conclusions: Mesial temporal lobe epilepsy patients showed widespread cortical thinning before and after anterior temporal lobectomy. Progressive cortical thinning mainly existed in neighboring regions of resection. Postoperative cortical thickening may indicate cortical remodeling after successful surgery.
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http://dx.doi.org/10.1111/ane.13631DOI Listing
May 2022

Neuromyelitis Optica Spectrum Disorder With Anti-Aquaporin-4 Antibody: Outcome Prediction Models.

Front Immunol 2022 31;13:873576. Epub 2022 Mar 31.

Department of Neurology, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, China.

Background: Recognizing the predictors of disease relapses in patients with anti-aquaporin-4 antibody (AQP4-ab)-positive neuromyelitis optica spectrum disorder (NMOSD) is essential for individualized treatment strategy. We aimed to identify the factors that predicted relapses among patients with AQP4-ab-positive NMOSD, develop outcome prediction models, and validate them in a multicenter validation cohort.

Methods: Between January 2015 and December 2020, 820 patients with NMOSD were registered at Huashan Hospital. We retrospectively reviewed their medical records, and included 358 AQP4-ab-positive patients with 1135 treatment episodes. Univariate and multivariate analyses were used to explore the predictors of relapse, severe visual or motor disability during follow-up. A model predicting the 1- and 2-year relapse-free probability was developed and validated in an external validation cohort of 92 patients with 213 treatment episodes.

Results: Lower serum AQP4-ab titer (< 1:100), higher Expanded Disability Status Scale (EDSS) score at onset (≥ 2.5), and use of intravenous methylprednisolone (IVMP) at the first attack predicted an overall lower annualized relapse rate. Older age (> 48 years), optic neuritis at onset, and higher onset EDSS score (≥ 2.5) significantly increased the risk for blindness, while IVMP at the first attack and maintenance therapy reduced the risk for blindness. Myelitis at onset increased the possibility of motor disability (EDSS ≥ 6.0), severe motor disability or death (EDSS ≥ 8.0), while maintenance therapy reduced these possibilities. Anderson and Gill model identified that the risk factors predicting recurrent relapses under certain treatment status were female gender, high AQP4-ab titer (≥ 1:100), previous attack under same therapy, lower EDSS score at treatment initiation (< 2.5), and no maintenance therapy or oral prednisone lasting less than 6 months. A nomogram using the above factors showed good discrimination and calibration abilities. The concordance indexes in the primary and validation cohort were 0.66 and 0.65, respectively.

Conclusion: This study reports the demographic, clinical and therapeutic predictors of relapse, and severe visual or motor disability in NMOSD. Early identification of patients at risk of unfavorable outcomes is of paramount importance to inform treatment decisions.
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http://dx.doi.org/10.3389/fimmu.2022.873576DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9012141PMC
April 2022

Response of the aerobic denitrifying phosphorus accumulating bacteria Pseudomonas psychrophila HA-2 to low temperature and zinc oxide nanoparticles stress.

Bioresour Technol 2022 Jun 13;354:127162. Epub 2022 Apr 13.

College of Environmental Sciences and Engineering, Peking University, Key Laboratory of Water and Sediment Sciences, Ministry of Education, Beijing 100871, PR China; State Environmental Protection Key Laboratory of All Material Fluxes in River Ecosystems, Beijing 100871, China. Electronic address:

Performance and molecular changes of an aerobic denitrifying phosphorus accumulating bacteria Pseudomonas psychrophila HA-2 have been investigated under different temperatures and ZnO nanoparticles (NPs) exposures. Strain HA-2 removed 95.7% of total nitrogen (TN) and 24.6% of phosphorus at 10 °C, which was attributed to the joint up-regulation of intracellular energy metabolism and ribosome. Moreover, with the increase of ZnO NPs from 0 to 100 mg/L, TN and phosphurs removal efficiencies decreased from 95.7% to 44.5% and 24.6% to 6.8% at 10 °C, respectively, whereas phosphorus removal rate increased from 10.5% to 24.5% at 20 °C. Further transcriptomics and proteomics revealed that significant down-regulation of purine and amino acid metabolisms was the main reason for the inhibitory effect at 10 °C, while the up-regulation of antioxidant pathways and functional genes expressions was responsible for the promoted phosphorus accumulation at 20 °C. This study provides a potential solution for improving biological nutrients removal processes in winter months.
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http://dx.doi.org/10.1016/j.biortech.2022.127162DOI Listing
June 2022

The NDT80-like transcription factor CmNdt80a affects the conidial formation and germination, mycoparasitism, and cell wall integrity of Coniothyrium minitans.

J Appl Microbiol 2022 Apr 16. Epub 2022 Apr 16.

Institute of Plant Protection, Sichuan Academy of Agricultural Sciences, Chengdu, China.

Aims: NDT80, a known transcriptional factor, regulates various targets, including regulation of meiosis, stress responses, filamentous growth, sexual development, biofilm formation, drug resistance, and virulence. But the numbers and roles of Ndt80-like genes in different fungi are highly variable. This study aims to address the numbers and functions of Ndt80-like genes in Coniothyrium minitans, a well-known biocontrol agent against Sclerotinia diseases.

Methods And Results: Here, two genes (CmNdt80a and CmNdt80b) encoding NDT80-like proteins were obtained by searching the genomic sequence of C. minitans. RT-PCR analysis showed that both CmNdt80 genes were constitutively expressed in C. minitans from the hyphal growth stage (48 hpi) to the pycnidial maturation stage (120 hpi). The roles of CmNdt80a and CmNdt80b in C. minitans were verified through gene knockout and complementation experiments. The results showed that the ΔCmNdt80a mutants exhibited a lighter colour and normal growth rate on potato dextrose agar plates. The ability of the ΔCmNdt80a mutants to produce conidia and parasitize the colony and sclerotia of Sclerotinia sclerotiorum was markedly diminished compared with the wild-type strain and complemented strain. Interestingly, the ΔCmNdt80a mutant showed less sensitivity to cell wall stressors and cell wall-degrading enzymes, and had thicker cell walls than the wild-type strain and complemented strain. However, no phenotypic consequences were discovered for the deletion of CmNdt80b.

Conclusions: Two Ndt80-like genes, CmNdt80a and CmNdt80b, were present in C. minitans. CmNdt80a, but not CmNdt80b, plays a crucial role in conidiogenesis, mycoparasitism, and cell wall integrity.

Significance And Impact Of The Study: Understanding the mechanisms that regulate the conidiation and parasitism of C. minitans could help us to more efficiently utilize this biological control agent and advance our knowledge of fungal biology.
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http://dx.doi.org/10.1111/jam.15575DOI Listing
April 2022

Vitamin C status and its change in relation to glucose-lipid metabolism in overweight and obesity patients following laparoscopic sleeve gastrectomy.

Eur J Clin Nutr 2022 Apr 14. Epub 2022 Apr 14.

Department of Endocrinology and Metabolism, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, Shanghai, 200072, China.

Objectives: Aimed to demonstrate the association of VC and metabolism in the obesity or overweight and determine VC changes after laparoscopic sleeve gastrectomy (LSG).

Methods: A total of 253 overweight or people with obesity were recruited, including 61 with LSG. They were divided into group A (VC < 34 ug/ml) and group B (VC ≥ 34 ug/ml). Glucose-lipid metabolic parameters were compared, and VC status before and 6 and 12 months after LSG were measured.

Results: (1) Body weight, body mass index (BMI), neck circumference (NC), waist circumference (WC), hip circumference (HC), waist-to-hip ratio, heart rate (HR), diastolic systolic pressure (DBP), 2-hour postprandial glucose (2h-BG), fasting insulin (FINS), 2-hour postprandial insulin (2h-INS), glycosylated hemoglobin (HBG), homeostasis model of insulin resistance (HOMA-IR), total cholesterol (TCH), triglyceride (TG) and free fatty acid (FFA) were higher while high-density lipoprotein (HDL-C) was lower in group A than group B (p < 0.05). (2) VC was negatively correlated with body weight, BMI, NC, WC and HC, HR, SBP, DBP, and 2h-BG, FINS, 2h-INS, HGB, HOMA-IR, TG and FFA, while positively with HDL-C (p < 0.05). (3) Patients with obesity or hypertriglyceridemia or low HDL-C had lower VC than corresponding group. (p < 0.05). (4) Logistic regression analysis showed that VC was the independent risk factor of hypertriglyceridemia, obesity and low HDL-C 5) VC concentrations were slightly increased in 6 months after LSG, and unchanged in 12 months after LSG.

Conclusion: VC was closely associated with glucose-lipid metabolism, and may play a protective role in metabolic disorders. LSG would not worsen the VC status or deficiency.
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http://dx.doi.org/10.1038/s41430-022-01134-1DOI Listing
April 2022

Identification of multimodal brain imaging association via a parameter decomposition based sparse multi-view canonical correlation analysis method.

BMC Bioinformatics 2022 Apr 12;23(Suppl 3):128. Epub 2022 Apr 12.

School of Automation, Northwestern Polytechnical University, Xi'an, China.

Background: With the development of noninvasive imaging technology, collecting different imaging measurements of the same brain has become more and more easy. These multimodal imaging data carry complementary information of the same brain, with both specific and shared information being intertwined. Within these multimodal data, it is essential to discriminate the specific information from the shared information since it is of benefit to comprehensively characterize brain diseases. While most existing methods are unqualified, in this paper, we propose a parameter decomposition based sparse multi-view canonical correlation analysis (PDSMCCA) method. PDSMCCA could identify both modality-shared and -specific information of multimodal data, leading to an in-depth understanding of complex pathology of brain disease.

Results: Compared with the SMCCA method, our method obtains higher correlation coefficients and better canonical weights on both synthetic data and real neuroimaging data. This indicates that, coupled with modality-shared and -specific feature selection, PDSMCCA improves the multi-view association identification and shows meaningful feature selection capability with desirable interpretation.

Conclusions: The novel PDSMCCA confirms that the parameter decomposition is a suitable strategy to identify both modality-shared and -specific imaging features. The multimodal association and the diverse information of multimodal imaging data enable us to better understand the brain disease such as Alzheimer's disease.
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http://dx.doi.org/10.1186/s12859-022-04669-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9006414PMC
April 2022

Association Between Abdominal Adipose Tissue Distribution and Obstructive Sleep Apnea in Chinese Obese Patients.

Front Endocrinol (Lausanne) 2022 24;13:847324. Epub 2022 Mar 24.

Department of Endocrinology and Metabolism, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, Shanghai, China.

Purpose: Factors related to the occurrence of obstructive sleep apnea syndrome (OSAS) in obesity have not been fully clarified. The aim of this study was to identify the association between OSAS and abdominal fat distribution in a cohort of Chinese obese patients.

Methods: This cross-sectional study collected demographic data of 122 obese patients who were admitted into the in-patient unit of the Department of Endocrinology, Shanghai Tenth People's Hospital from July 2018 to January 2021. OSAS was diagnosed based on the results of overnight polysomnography, and the abdominal fat distribution was measured by bioelectrical impedance analysis (BIA). Univariate and multivariate logistic regression analyses were used to investigate the association between OSAS and the distribution of abdominal fat.

Results: (1) The mean age (SD) of the obese patients included was 32.44 (11.81) years old, and the overall incidence rate of OSAS was 51.06%. Twenty-four (25.53%) patients had mild OSAS, 10 (10.64%) had moderate OSAS, and 14 (14.89%) had severe OSAS. The apnea hypopnea index (AHI) of men was significantly higher than that of women (5.50, interquartile range (IQR) 3.80-30.6 vs. 4.2, IQR 1.4-12 events/h,  = 0.014). Meanwhile, men had a significantly higher visceral fat area when compared with women (180.29 ± 51.64 vs. 143.88 ± 53.42 cm,  = 0.002). (2) Patients with OSAS had a significantly higher waist circumference, fasting plasma glucose, 2 h postprandial plasma glucose, glycated hemoglobin, and visceral fat area than patients without OSAS (all  < 0.05). (3) AHI was significantly positively associated with BMI, neck circumference, waist circumference, and visceral fat area ( = 0.306,  = 0.003;  = 0.380,  < 0.001;  = 0.328,  = 0.002;  = 0.420,  < 0.001) but not with subcutaneous fat area ( = 0.094). Multivariate analysis demonstrated that abdominal fat area and fasting plasma glucose were independent risk factors for OSAS (odds ratio, 1.016; 95% confidence interval, 1.005-1,026,  = 0.005; odds ratio, 1.618; 95% confidence interval, 1.149-2.278,  = 0.006).

Conclusions: In obese patients, the abdominal visceral adipose deposit but not the subcutaneous fat area was associated with OSAS and was an independent risk factor for OSAS. Therefore, improving the distribution of abdominal fat may contribute to alleviating the severity of OSAS in obesity.
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http://dx.doi.org/10.3389/fendo.2022.847324DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8988152PMC
April 2022

Relationship of Vitamin A and Thyroid Function in Individuals With Obesity and After Laparoscopic Sleeve Gastrectomy.

Front Nutr 2022 15;9:824193. Epub 2022 Mar 15.

Department of Endocrinology and Metabolism, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, Shanghai, China.

Vitamin A deficiency (VAD) occurs in obesity and may be associated with thyroid dysfunction. We aimed to investigate the association of VA with thyroid function in obesity and after laparoscopic sleeve gastrectomy (LSG). Nine hundred and seventy-six obese subjects were enrolled for this study and were divided into VAD, marginal vitamin A deficiency (MVAD), and vitamin A normal (NVA) groups. VAD was defined as VA ≤ 200 ng/ml, MVAD was defined as VA > 200 but <300 ng/ml, and NVA was defined as VA ≥ 300 ng/ml. Thyroid function was compared among groups and the relationship of VA and thyroid function was analyzed. Two hundred and forty-four of the 976 obese subjects underwent LSG, and the change in thyroid function and VA at 3, 6, and 12 months after surgery was measured. Results showed that 37% of all the subjects had subclinical hypothyroidism (SH), and the SH group had lower VA levels than the non-SH group ( = 0.008). Forty-nine percent of all the subjects had MVAD, 9% had VAD, while the MVAD or VAD group had lower FT4 than the NVA group ( = 0.005 and = 0.001). The VAD group also had higher TSH than NVA group ( = 0.037). VA was significantly negatively associated with TSH ( = -0.151, = 0.006) and positively associated with FT4 ( = 0.228, < 0.001). TSH was significantly decreased at 3, 6, and 12 months (3M: from 4.43 ± 2.70 to 2.63 ± 1.46 mU/l, < 0.001; 6M: from 4.43 ± 2.70 to 3.84 ± 2.34 mU/l, = 0.041; 12M: from 4.43 ± 2.70 to 2.85 ± 1.68 mU/l, = 0.024). After LSG surgery, VA levels were slightly increased, when compared to pre-surgery levels, at 3, 6, and 12 months (3M: from 262.57 ± 68.19 to 410.33 ± 76.55 ng/ml, = 0.065; 6M: from 262.57 ± 68.19 to 281.36 ± 93.23 ng/ml, = 0.343; 12M: from 262.57 ± 68.19 to 300.37 ± 86.03 ng/ml, = 0.083). SH group also had lower TSH and higher VA than the non-SH group at 3 months post-surgery [TSH: -1.4(-2.3, -0.3) vs. -0.2(-0.8, -0.2) mU/l, < 0.001; VA: 163.99 ± 32.58 vs. 121.69 ± 27.59 ng/ml, = 0.044]. In conclusion VA, which is related to thyroid hormone production, protects against thyroid dysfunction in obese subjects. The improvement of thyroid function in subjects with SH after LSG may be related to the increased VA levels observed post-surgery.

Clinical Trial Registration: ClinicalTrial.gov ID: NCT04548232.
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http://dx.doi.org/10.3389/fnut.2022.824193DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8990331PMC
March 2022

Efficacy and safety of azathioprine, mycophenolate mofetil, and reduced dose of rituximab in neuromyelitis optica spectrum disorder.

Eur J Neurol 2022 Apr 10. Epub 2022 Apr 10.

Department of Neurology, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, China.

Background And Purpose: Data regarding the efficacy and safety of currently widely available preventive therapies in neuromyelitis optica spectrum disorder (NMOSD) are needed. We compared the efficacy and safety of azathioprine (AZA), mycophenolate mofetil (MMF), and reduced dose of rituximab (RTX) in NMOSD based on a large multicenter retrospective cohort.

Methods: Patients with aquaporin 4 (AQP4) antibody-positive NMOSD with AZA (n = 167), MMF (n = 131), or RTX (n = 55) as initial preventive treatment were included. The main outcome was the occurrence of relapse after the initiation of immunotherapy. Secondary outcomes were annual relapse rate, disability accumulation, drug persistence, and adverse events.

Results: The median follow-up time of the 353 patients was 30.3 months. The regimen of RTX was 100 mg on Day 1 and 500 mg on Day 2, followed by 500 mg every 6 months. The proportions of patients with concomitant steroid therapy at baseline were 96.4%, 95.4%, and 76.4% in the AZA, MMF, and RTX groups. Risk of relapse was significantly reduced in patients treated with RTX compared with those treated with AZA (hazard ratio [HR] = 4.40, 95% confidence interval [CI] = 1.41-13.80, p = 0.011) or MMF (HR = 5.20, 95% CI = 1.60-16.86, p = 0.006) after adjusting for potential confounding variables. Drug discontinuations were less likely on RTX than AZA (HR = 2.22, 95% CI = 1.34-3.66, p = 0.002). RTX exhibited lower incidence of adverse events (32.7%) than AZA (62.3%, p < 0.001).

Conclusions: We provide Class III evidence that reduced dose of RTX is superior to AZA and MMF as initial treatment to reduce the risk of relapse and is better tolerated than AZA in Chinese patients with AQP4 antibody-positive NMOSD.
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http://dx.doi.org/10.1111/ene.15355DOI Listing
April 2022

Brain morphometric features predict medication response in youth with bipolar disorder: a prospective randomized clinical trial.

Psychol Med 2022 Apr 8:1-11. Epub 2022 Apr 8.

Department of Psychiatry and Behavioral Neuroscience, University of Cincinnati College of Medicine, Cincinnati45219, OH, USA.

Background: Identification of treatment-specific predictors of drug therapies for bipolar disorder (BD) is important because only about half of individuals respond to any specific medication. However, medication response in pediatric BD is variable and not well predicted by clinical characteristics.

Methods: A total of 121 youth with early course BD (acute manic/mixed episode) were prospectively recruited and randomized to 6 weeks of double-blind treatment with quetiapine (n = 71) or lithium (n = 50). Participants completed structural magnetic resonance imaging (MRI) at baseline before treatment and 1 week after treatment initiation, and brain morphometric features were extracted for each individual based on MRI scans. Positive antimanic treatment response at week 6 was defined as an over 50% reduction of Young Mania Rating Scale scores from baseline. Two-stage deep learning prediction model was established to distinguish responders and non-responders based on different feature sets.

Results: Pre-treatment morphometry and morphometric changes occurring during the first week can both independently predict treatment outcome of quetiapine and lithium with balanced accuracy over 75% (all p < 0.05). Combining brain morphometry at baseline and week 1 allows prediction with the highest balanced accuracy (quetiapine: 83.2% and lithium: 83.5%). Predictions in the quetiapine and lithium group were found to be driven by different morphometric patterns.

Conclusions: These findings demonstrate that pre-treatment morphometric measures and acute brain morphometric changes can serve as medication response predictors in pediatric BD. Brain morphometric features may provide promising biomarkers for developing biologically-informed treatment outcome prediction and patient stratification tools for BD treatment development.
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http://dx.doi.org/10.1017/S0033291722000757DOI Listing
April 2022

Using graph convolutional network to characterize individuals with major depressive disorder across multiple imaging sites.

EBioMedicine 2022 Apr 1;78:103977. Epub 2022 Apr 1.

Huaxi MR Research Center (HMRRC), Department of Radiology, West China Hospital of Sichuan University, Chengdu, China; Department of Radiology, West China Xiamen Hospital of Sichuan University, Xiamen, Fujian, China. Electronic address:

Background: Establishing objective and quantitative neuroimaging biomarkers at individual level can assist in early and accurate diagnosis of major depressive disorder (MDD). However, most previous studies using machine learning to identify MDD were based on small sample size and did not account for the brain connectome that is associated with the pathophysiology of MDD. Here, we addressed these limitations by applying graph convolutional network (GCN) in a large multi-site MDD dataset.

Methods: Resting-state functional MRI scans of 1586 participants (821 MDD vs. 765 controls) across 16 sites of Rest-meta-MDD consortium were collected. GCN model was trained with individual whole-brain functional network to identify MDD patients from controls, characterize the most salient regions contributing to classification, and explore the relationship between topological characteristics of salient regions and clinical measures.

Findings: GCN achieved an accuracy of 81·5% (95%CI: 80·5-82·5%, AUC: 0·865), which was higher than other common machine learning classifiers. The most salient regions contributing to classification were primarily identified within the default mode, fronto-parietal, and cingulo-opercular networks. Nodal topologies of the left inferior parietal lobule and left dorsolateral prefrontal cortex were associated with depressive severity and illness duration, respectively.

Interpretation: These findings based on a large, multi-site dataset support the feasibility and effectiveness of GCN in characterizing MDD, and also illustrate the potential utility of GCN for enhancing understanding of the neurobiology of MDD by detecting clinically-relevant disruption in functional network topology.

Funding: This study was supported by the National Natural Science Foundation of China (Grant Nos. 81621003, 82027808, 81820108018).
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http://dx.doi.org/10.1016/j.ebiom.2022.103977DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8983334PMC
April 2022

Disrupted Brain Functional Network Topology in Essential Tremor Patients With Poor Sleep Quality.

Front Neurosci 2022 10;16:814745. Epub 2022 Mar 10.

Department of Neurology, West China Hospital, Sichuan University, Chengdu, China.

Sleep disturbances, especially poor quality of sleep (QoS), are common among essential tremor (ET) patients and may have adverse effects on their quality of life, but the etiology driving the poor QoS in these individuals remains inadequately understood. Few data are available on the neuroimaging alterations of ET with poor QoS. Thirty-eight ET patients with poor QoS (SleET), 48 ET patients with normal QoS (NorET), and 80 healthy controls (HCs) participated in this study. All subjects underwent a 3.0-T magnetic resonance imaging (MRI) scan for resting-state functional MRI data collection. Then, the whole-brain functional connectome was constructed by thresholding the partial correlation matrices of 116 brain regions. Graph theory and network-based statistical analyses were performed. We used a non-parametric permutation test for group comparisons of topological metrics. Partial correlation analyses between the topographical features and clinical characteristics were conducted. The SleET and NorET groups exhibited decreased clustering coefficients, global efficiency, and local efficiency and increased the characteristic path length. Both of these groups also showed reduced nodal degree and nodal efficiency in the left superior dorsolateral frontal gyrus, superior frontal medial gyrus (SFGmed), posterior cingulate gyrus (PCG), lingual gyrus, superior occipital gyrus, right middle occipital gyrus, and right fusiform gyrus. The SleET group additionally presented reduced nodal degrees and nodal efficiency in the right SFGmed relative to the NorET and HC groups, and nodal efficiency in the right SFGmed was negatively correlated with the Pittsburgh Sleep Quality Index score. The observed impaired topographical organizations of functional brain networks within the central executive network (CEN), default mode network (DMN), and visual network serve to further our knowledge of the complex interactions between tremor and sleep, adding to our understanding of the underlying neural mechanisms of ET with poor QoS.
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http://dx.doi.org/10.3389/fnins.2022.814745DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8960629PMC
March 2022

Moderate hypofractionated helical tomotherapy for older patients with localized prostate cancer: long-term outcomes of a phase I-II trial.

Radiol Oncol 2022 Mar 28;56(2):216-227. Epub 2022 Mar 28.

Medical School of Chinese PLA, Beijing, China.

Background: Our previous study showed that two different regimens of moderate hypofractionated radiotherapy (HFRT) delivered with helical tomotherapy (HT) are well tolerated in older prostate cancer patients. We provide a longterm efficacy and toxicity after > 7 years of follow-up.

Patients And Methods: The study recruited 33 patients from February 2009 to July 2011 (76 Gy/34F; Group-1); and 34 from July 2011 to February 2014 (71.6 Gy/28F; 50.4 Gy/25F for the risk of pelvic lymph nodes involvement (LNI) >15%; Group-2). The primary outcomes were biochemical failure (BF), biochemical failure and clinical disease failure (BCDF), progression-free survival (PFS), overall survival (OS), late genitourinary (GU) and gastrointestinal (GI) toxicity.

Results: The average ages of two groups were 80 and 77 years and the proportions of patients with LNI > 15% were 69.7% and 73.5%, respectively. At the final follow-up in February 2020, 27.3% and 20.6% cases experienced BF, with a median time until BF of 3.3 years. A total of 38.8% patients reached primary endpoints, in which 18 deaths were reported BCDF events (45.5% . 32.4%, p = 0.271). There was no significant difference in 7-year PFS (68.6% . 74.8%, p = 0.591), BCDF (45.5% . 32.4%, p = 0.271) and OS (71.9% . 87.5%, p = 0.376) for full set analysis and for subgroup analysis (all p > 0.05). The incidence of grade ≥ 2 late GU (6.2% . 6.3%, p = 0.127) and GI toxicities (9.4% . 15.6%, p = 0.554) was comparable.

Conclusions: In older patients with localized prostate cancer, two moderate hypofractionated regimens were all well tolerated with similar, mild late toxicities and satisfactory survival, without necessity of prophylactic pelvic node irradiation.
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http://dx.doi.org/10.2478/raon-2022-0011DOI Listing
March 2022

Pretreatment topological disruptions of whole-brain networks exist in childhood absence epilepsy: A resting-state EEG-fMRI study.

Epilepsy Res 2022 May 16;182:106909. Epub 2022 Mar 16.

Department of Neurology, West China Hospital, Sichuan University, Cheng du, Sichuan, PR China. Electronic address:

Purpose: Childhood absence epilepsy (CAE) is associated with functional changes in specific brain regions and connections. However, little is known about the topological properties of the functional brain connectome in drug naive CAE.

Methods: We adopted the resting-state EEG-fMRI and graph theoretic approach to investigate both local and global brain functional network properties of drug naive CAE during interictal resting state compared with healthy control. In addition, we computed the partial correlation coefficient to estimate the correlation between the functional network metrics and the measured disease duration or the age at seizure onset.

Results: The functional connectome in drug naive CAE showed decreased small-worldness and normalized clustering coefficient at the global level. At the nodal level, decreased nodal centralities were mainly in bilateral prefrontal-thalamocortical circuit and increased nodal centralities mainly in left hippocampus and right middle temporal gyrus (p < 0.05). In addition, the duration of the epilepsy was significantly correlated with the nodal efficiency in left middle frontal gyrus (r = -0.627, p = 0.012).

Conclusion: The pretreatment topological disruptions of whole-brain networks exist in drug naive patients with CAE and the functional impairment mainly involve the prefrontal-thalamocortical circuit. These findings in the homogeneous group of CAE indicate that the aberrant topological organization of functional brain network is an intrinsic feature of CAE and provide topologic insights into understanding the pathophysiological mechanisms of CAE.
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http://dx.doi.org/10.1016/j.eplepsyres.2022.106909DOI Listing
May 2022

Krill oil prevents lipopolysaccharide-evoked acute liver injury in mice through inhibition of oxidative stress and inflammation.

Food Funct 2022 Apr 4;13(7):3853-3864. Epub 2022 Apr 4.

Research Center of Translational Medicine, Jinan Central Hospital, Cheeloo College of Medicine, Shandong University, No.105 Jiefang Road, Jinan, Shandong, 250013, China.

Acute liver injury is a life-threatening syndrome that often results from the actions of viruses, drugs and toxins. Herein, the protective effect and potential mechanism of krill oil (KO), a novel natural product rich in long-chain n-3 polyunsaturated fatty acids bound to phospholipids and astaxanthin, on lipopolysaccharide (LPS)-evoked acute liver injury in mice were investigated. Male C57BL/6J mice were administered intragastrically with 400 mg kg KO or fish oil (FO) once per day for 28 consecutive days prior to LPS exposure (10 mg kg, intraperitoneally injected). The results revealed that KO pretreatment significantly ameliorated LPS-evoked hepatic dysfunction indicated by reduced serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities and attenuated hepatic histopathological damage. KO pretreatment also mitigated LPS-induced hepatic oxidative stress, as evidenced by decreased malondialdehyde (MDA) contents, elevated glutathione (GSH) levels, and elevated catalase (CAT) and superoxide dismutase (SOD) activities. Additionally, LPS-evoked overproduction of pro-inflammatory mediators in serum and the liver was inhibited by KO pretreatment. Furthermore, KO pretreatment suppressed LPS-induced activation of the hepatic toll-like receptor 4 (TLR4)/nuclear factor-kappa B (NF-κB)/NOD-like receptor family pyrin domain containing 3 (NLRP3) signaling pathway. Interestingly, the hepatoprotective effect of KO was superior to that of FO. Collectively, the current findings suggest that KO protects against LPS-evoked acute liver injury inhibition of oxidative stress and inflammation.
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http://dx.doi.org/10.1039/d1fo04136cDOI Listing
April 2022

Robotic adrenalectomy versus laparoscopic adrenalectomy for pheochromocytoma: a systematic review and meta-analysis.

Wideochir Inne Tech Maloinwazyjne 2022 Mar 13;17(1):1-8. Epub 2021 Jul 13.

Department of Urology, The Second Hospital of Hebei Medical University, Shijiazhuang, China.

Introduction: The application of robotic adrenalectomy (RA) has been increasing. However, there is still controversy about whether RA is more feasible than laparoscopic adrenalectomy (LA) for pheochromocytoma (PHEO).

Aim: To evaluate the efficacy and safety of RA vs. LA for PHEO.

Material And Methods: A literature search of the PubMed, Ovid, and Scopus databases was performed to identify eligible studies up to April 2021. All studies comparing RA versus LA for PHEO were included. Data were analysed using the Cochrane Collaboration's Review Manager (RevMan) 5.4 software.

Results: Overall, 4 studies including 386 patients (RA 155; LA 231) were included. RA might have larger tumour size (WMD = 0.72 cm, 95% CI: 0.31 to 1.13; p < 0.001). There were no statistically significant differences in operative time (WMD = -12.49 min, 95% CI: -29.50 to 4.52; p = 0.15), estimated blood loss (EBL) (WMD = -28.48 ml, 95% CI: -58.92, 1.95; p = 0.07), transfusion rate (OR = 0.70, 95% CI: 0.07 to 7.07; p = 0.77), or conversion rate (OR = 0.44, 95% CI: 0.07 to 2.88; p = 0.39). There were no significant differences between the 2 groups in terms of postoperative complications (OR = 1.06, 95% CI: 0.62 to 1.82; p = 0.84) and Clavien Dindo score ≥ 3 complications (OR = 1.15, 95% CI: 0.39 to 3.41; p = 0.80). Patients from the RA group could benefit from shorter length of hospital stay (WMD = -0.51 days, 95% CI -0.91 to -0.12; p = 0.01).

Conclusions: RA is a feasible, safe, and comparable treatment option for PHEO.
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http://dx.doi.org/10.5114/wiitm.2021.107764DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8886480PMC
March 2022

Anti-inflammatory and anti-apoptotic effects of Shaoyao decoction on X-ray radiation-induced enteritis of C57BL/6 mice.

J Ethnopharmacol 2022 Jun 2;292:115158. Epub 2022 Mar 2.

Oncology Department, Affiliated Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, 610072, China. Electronic address:

Ethnopharmacological Relevance: As a typical heat-clearing prescription, Shaoyao decoction (SYD) has a robust function of clearing viscera heat for the treatment of several intestinal discomfort symptoms. Clinical evidence indicated that it had the potential to cure radiation enteritis. However, its underlying mechanisms remain unclear.

Aim Of The Study: The present study was designed to probe the protective effects and the involved mechanisms of SYD on X-ray radiation-induced enteritis of C57BL/6 mice.

Materials And Methods: X-ray irradiation were used to establish the radiation enteritis model. Forty-eight male C57BL/6 mice (20 ± 2 g) were randomly divided into six groups: the control group, model group, dexamethasone group (DEX, 0.12 mg/kg) and SYD groups (0.12, 0.24 and 0.36 g/mL), respectively. All mice (except the control group) were intragastrically administrated for a continuous 7 days. H&E and Masson staining were employed to evaluate the morphological and collagen fibers changes of the colon. ELISA was performed to assess the levels of MDA, SOD, COX, LPS, IL-6, IL-1β and TNF-α in serum. Moreover, TUNEL fluorescence, western blot and qRT-PCR were used to detect the levels of apoptosis-related proteins and genes of Dclk-1, ATM, MRE-11, Bcl-2, Bax, Caspase-3, and Cyto-c. Furthermore, immunofluorescent staining was applied to detect the protein levels of p53 and Claudin-1 in colon.

Results: Treatment with SYD decreased the exfoliated and necrotic epithelial cells and prevent the proliferate from damaged fibrous tissue in the crypt layer of mucos. The levels of serum peroxidation and pro-inflammatory cytokines (MDA, COX, LPS, IL-6, IL-1β and TNF-α) were obviously inhibited, while SOD sharply increased in serum after administration. Moreover, SYD can significantly ameliorate the apoptosis of colon cells, evidenced by the reduced positive expression of TUNEL staining. Meanwhile, the results of qRT-PCR and western blot demonstrated that SYD can dramatically stimulate the expression of genes and proteins Dclk-1, ATM and MRE-11, thus promoting the expression of mitochondrial pro-apoptotic proteins Bax, Caspase-3 and Cyto-c, while increasing the level of anti-apoptotic protein Bcl-2. Furthermore, immunofluorescence revealed that SYD can notably decreased the protein level of p53 while reverse the reduction of Claudin-1.

Conclusions: These results indicated that radiation enteritis in C57BL/6 mice can be ameliorated by treatment with SYD. The potential protection mechanisms may be involved in ameliorating tissue fibrosis by decreasing inflammatory and apoptotic events.
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http://dx.doi.org/10.1016/j.jep.2022.115158DOI Listing
June 2022

Retraction notice to "G-5555 synergized miR-485-5p to alleviate cisplatin resistance in ovarian cancer cells via Pi3k/Akt signaling pathway" [J. Reprod. Immunol. 140C (2020) 103129].

J Reprod Immunol 2022 03 23;150:103495. Epub 2022 Feb 23.

Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Nantong University, Nantong, Jiangsu Province 226001, China.

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http://dx.doi.org/10.1016/j.jri.2022.103495DOI Listing
March 2022

Application of Platelet-Rich Fibrin Transplantation for Large Macular Hole.

Curr Eye Res 2022 Mar 22:1-7. Epub 2022 Mar 22.

Department of Ophthalmology, Renmin Hospital of Wuhan University, Wuhan, China.

Large idiopathic macular hole (MH) has a lower closure rate and poor visual prognosis. To increase the closure rate and the visual outcome in patients with large MH, we evaluate a technique of platelet-rich fibrin (PRF) membrane transplantation for the treatment of large MH. This was a prospective interventional study. Seventeen eyes of 17 patients (8 males and 9 females) with large MH (654.94 ± 188.08 μm) underwent pars plana vitrectomy and internal limiting membrane peeling followed by PRF transplantation between January 2019 and December 2020. The patients were followed up for at least 6 months. Preoperatively and postoperatively, all patients underwent comprehensive ophthalmic examinations, including best-corrected visual acuity (BCVA), intraocular pressure, slit-lamp biomicroscopy, indirect ophthalmoscopy, ultra-widefield retinal imaging, optical coherent tomography, and optical coherent tomography angiography. All the patients achieved complete and consistent hole closure after the surgery (100%). At 6 months follow-up, the external limiting membrane defects were observed in 3 eyes (17.64%), and the ellipsoid zone defects were observed in 10 eyes (58.82%). The preoperative BCVA was 1.21 ± 0.33 in logMAR. Postoperatively, the BCVA was significantly improved to 0.64 ± 0.22 in logMAR at 6 months ( < 0.001). The foveal avascular zone reduced significantly from 0.41 ± 0.08 mm to 0.26 ± 0.07 mm ( < 0.001). No complications were observed during or after the operation. All patients in this study have achieved good anatomic and functional results, which indicate that the application of PRF transplantation is an effective and safe technique for large MH.
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http://dx.doi.org/10.1080/02713683.2022.2029906DOI Listing
March 2022

DHA-Enriched Phospholipids and EPA-Enriched Phospholipids Alleviate Lipopolysaccharide-Induced Intestinal Barrier Injury in Mice a Sirtuin 1-Dependent Mechanism.

J Agric Food Chem 2022 Mar 17;70(9):2911-2922. Epub 2022 Feb 17.

Research Center of Translational Medicine, Jinan Central Hospital, Cheeloo College of Medicine, Shandong University, No. 105 Jiefang Road, Jinan, Shandong 250013, China.

Intestinal barrier dysfunction has emerged as a potential contributor to the development of several severe diseases. Herein, the effect and underlying mechanism of DHA-enriched phospholipids (DHA-PL) and EPA-enriched phospholipids (EPA-PL) on protecting against lipopolysaccharide (LPS)-induced intestinal barrier injury were elucidated. C57BL/6J male mice were fed an AIN-93G diet containing 1% DHA-PL or EPA-PL for 4 weeks and then were intraperitoneally injected with LPS (10 mg/kg) to cause intestinal barrier injury. The results manifested that DHA-PL and EPA-PL pretreatment balanced apoptosis and autophagy in intestinal epithelial cells and maintained intestinal tight junction integrity. Our findings also demonstrated that cotreatment with EX-527, a sirtuin 1 specific inhibitor, hindered the role of DHA-PL and EPA-PL against LPS-evoked intestinal barrier injury through reversing the inhibitory action of them on NF-κB and MAPKs activation as well as their potentiating actions on Nrf2 nuclear translocation. Overall, DHA-PL and EPA-PL alleviated LPS-mediated intestinal barrier injury inactivation of the NF-κB and MAPKs pathways as well as activating the Nrf2 antioxidant pathway up-regulating sirtuin 1.
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http://dx.doi.org/10.1021/acs.jafc.1c07761DOI Listing
March 2022

The effects of angiotensin I-converting enzyme inhibitory peptide VGINYW and the hydrolysate of α-lactalbumin on blood pressure, oxidative stress and gut microbiota of spontaneously hypertensive rats.

Food Funct 2022 Mar 7;13(5):2743-2755. Epub 2022 Mar 7.

State Key Laboratory of Bioreactor Engineering; Department of Food Science and Technology, School of Biotechnology, East China University of Science and Technology, Shanghai 200237, P. R. China.

VGINYW is a highly active angiotensin I-converting enzyme (ACE) inhibitory peptide discovered from α-lactalbumin by an - high throughput screening strategy. The aim of this study was to evaluate the antihypertensive effect of the peptide and the α-lactalbumin hydrolysates under 3 kDa (LH-3k), and illustrate the possible mechanism in spontaneously hypertensive rats (SHRs). SHRs were administered with VGINYW and LH-3k at doses of 5 mg per kg BW and 100 mg per kg BW, respectively. VGINYW and LH-3k could markedly decrease the systolic blood pressure (SBP) of the SHRs, and the maximal drops of 21 mmHg (2 h after administration) and 17 mmHg (4 h after administration) were achieved during the 8 hour test, respectively. When the agents were given once per day for 4 weeks, they caused a long-term decrease of 16 mmHg of SBP. VGINYW and LH-3k control the blood pressure through regulating the renin-angiotensin system by inhibiting the ACE activity and diminishing the angiotensin II level, and further upregulating the expression levels of the angiotensin-converting enzyme 2 and angiotensin type 2 receptor, and downregulating the expression of the angiotensin type 1 receptor. VGINYW and LH-3k could notably ameliorate the oxidative stress in the SHR as well. It is more important that the gavage of VGINYW and LH-3k could alleviate hypertension-associated intestinal microbiota dysbiosis by recovering the diversity of the gut microbiota and altering the key floras which are short chain fatty acid producers. In conclusion, VGINYW and LH-3k are effective functional ingredients for blood pressure control.
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http://dx.doi.org/10.1039/d1fo03570cDOI Listing
March 2022
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