Publications by authors named "Lei Dong"

986 Publications

Fetal dose from proton pencil beam scanning craniospinal irradiation during pregnancy: a Monte Carlo study.

Authors:
Yeon Soo Yeom Keith Tchadwick Griffin Matthew M Mille Choonik Lee Shannon O'Reilly Lei Dong Jae Won Jung Choonsik Lee

Phys Med Biol 2022 Jan 13. Epub 2022 Jan 13.

Radiation Epidemiology Branch, National Cancer Institute, 9609 Medical Center Dr, Bethesda, Maryland, 20850 , UNITED STATES.

Objective: We conducted a Monte Carlo study to comprehensively investigate the fetal dose resulting from proton pencil beam scanning (PBS) craniospinal irradiation (CSI) during pregnancy.

Approach: The gestational-age dependent pregnant phantom series developed at the University of Florida (UF) were converted into DICOM-RT format (CT images and structures) and imported into a treatment planning system (TPS) (Eclipse v15.6) commissioned to a IBA PBS nozzle. A proton PBS CSI plan (prescribed dose: 36 Gy) was created on the phantoms. The TOPAS MC code was used to simulate the proton PBS CSI on the phantoms, for which MC beam properties at the nozzle exit (spot size, spot divergence, mean energy, and energy spread) were matched to IBA PBS nozzle beam measurement data. We calculated mean absorbed doses for 28 organs and tissues and whole body of the fetus at eight gestational ages (8, 10, 15, 20, 25, 30, 35, and 38 weeks). For contextual purposes, the fetal organ/tissue doses from the treatment planning CT scan of the mother's head and torso were estimated using the National Cancer Institute dosimetry system for CT (NCICT, Version 3) considering a low-dose CT protocol (CTDIvol: 8.97 mGy).

Main Results: The majority of the fetal organ/tissue doses from the proton PBS CSI treatment fell within a range of 3 to 6 mGy. The fetal organ/tissue doses for the 38-week phantom showed the largest variation with the doses ranging from 2.9 mGy (adrenals) to 8.2 mGy (eye lenses) while the smallest variation ranging from 3.2 mGy (oesophagus) to 4.4 mGy (brain) was observed for the doses for the 20-week phantom. The fetal whole-body dose ranged from 3.7 mGy (25 weeks) to 5.8 mGy (8 weeks). Most of the fetal doses from the planning CT scan fell within a range of 7 to 13 mGy, approximately 2-to-9 times lower than the fetal dose equivalents of the proton PBS CSI treatment (assuming a quality factor of 7).

Significance: The fetal organ/tissue doses observed in the present work will be useful for one of the first clinically informative predictions on the magnitude of fetal dose during proton PBS CSI during pregnancy.
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http://dx.doi.org/10.1088/1361-6560/ac4b38DOI Listing
January 2022

E3 ligase Smurf1 protects against misfolded SOD1 in neuronal cells by promoting its K63 ubiquitylation and aggresome formation.

Authors:
Lei Dong Liqun Liu Yang Li Wenxuan Li Liying Zhou Qin Xia

Hum Mol Genet 2022 Jan 12. Epub 2022 Jan 12.

School of Life Science, Beijing Institute of Technology, Beijing, 100081, China.

K63-linked polyubiquitination of the neurodegenerative disease-associated misfolded protein copper-zinc superoxide dismutase (SOD1) is associated with the formation of inclusion bodies. Highly expressed E3 ligase Smurf1 promotes cellular homostasis through the enhanced capability of aggregate degradation. However, it is not well explored the role of Smurf1 in the dynamics of SOD1 aggresomes. In this study, we report that Smurf1 promotes the recruitment of SOD1 to form aggresomes. Mechanistically, Smurf1 interacts with mutant SOD1 to promote aggresome formation by modification of its K63-linked polyubiquitination. Moreover, overexpressed Smurf1 enhances mutant SOD1 aggresome formation and autophagic degradation to prevent cell death. Thus, our data suggest that Smurf1 plays an important role in attenuating protein misfolding-induced cell toxicity by both driving the sequestration of misfolded SOD1 into aggresomes and autophagic degradation.
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http://dx.doi.org/10.1093/hmg/ddac008DOI Listing
January 2022

Ulinastatin improves renal microcirculation by protecting endothelial cells and inhibiting autophagy in a septic rat model.

Authors:
Tian Li Xiaojun Ji Jingfeng Liu Xinjie Guo Ran Pang Haizhou Zhuang Lei Dong Meili Duan Ang Li

Kidney Blood Press Res 2022 Jan 11. Epub 2022 Jan 11.

Introduction: Increased permeability of the renal capillaries is a common consequence of sepsis-associated acute kidney injury. Vascular endothelial (VE)-cadherin is a strictly endothelial-specific adhesion molecule that can control the permeability of the blood vessel wall. Additionally, autophagy plays an important role in maintaining cell stability. Ulinastatin, a urinary trypsin inhibitor, attenuates the systemic inflammatory response and visceral vasopermeability. However, it is uncertain whether ulinastatin can improve renal microcirculation by acting on the endothelial adhesion junction.

Methods: We observed the effect of ulinastatin in a septic rat model using contrast-enhanced ultrasonography (CEUS) to evaluate the perfusion of the renal cortex and medulla. Male adult Sprague-Dawley rats were subjected to cecal ligation and puncture and divided into the sham, sepsis, and ulinastatin groups. Ulinastatin (50,000 U/kg) was injected into the tail vein immediately after the operation. The CEUS was performed to evaluate the renal microcirculation perfusion at 3, 6, 12, and 24 hours after the operation. Histological staining was used to evaluate kidney injury scores. Western blot (WB) was used to quantify the expression of VE-cadherin, LC3II, and inflammatory factors [interleukin -1β (IL-1β), interleukin -6 (IL-6), and tumor necrosis factor-α (TNF-α)] in kidney tissue, and enzyme-linked immunosorbent assay (ELISA) detected serum inflammatory factors and kidney function and early kidney injury biomarker levels.

Results: Compared with the sham group, ulinastatin reduced the inflammatory response, inhibited autophagy, maintained the expression of VE-cadherin, and meliorated cortical and medullary perfusion.

Conclusion: Ulinastatin effectively protects the adhesion junction and helps ameliorate the perfusion of kidney capillaries during sepsis by the inhibition of autophagy and the expression of inflammatory factors.
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http://dx.doi.org/10.1159/000521648DOI Listing
January 2022

Qualitative and quantitative analysis of Alpiniae Oxyphyllae fructus by HPLC coupled to Fourier transform-ion cyclotron resonance mass spectrometry.

Authors:
Yu Zhang Lei Dong Qingyu Zhang Jing Han Wenwen Sui Ziyun Wei Zan Zhang Yichen Tong Sijie Wang Fei Han

J Sep Sci 2022 Jan 11. Epub 2022 Jan 11.

School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenhe District, Shenyang, 110016, China.

Alpiniae Oxyphyllae Fructus, as a homology of medicine and food, has been widely used in China for thousands of years. However, the existing qualitative and quantitative methods are difficult to evaluate the quality of Alpiniae Oxyphyllae Fructus samples from multiple sources. In this paper, an HPLC fingerprint was established for assessing the quality of Alpiniae Oxyphyllae Fructus from different areas. Then, HPLC was coupled to Fourier transform-ion cyclotron resonance mass spectrometry for characterization of the chemical compositions in Alpiniae Oxyphyllae Fructus. In fingerprint analysis, 54 common peaks were confirmed and 6 chromatographic peaks of them were identified. The similarity of 14 samples from different areas was between 0.990 and 1.000. Moreover, a total of 30 chemical components were characterized by high-performance liquid chromatography coupled to fourier transform-ion cyclotron resonance mass spectrometer method and 6 compounds of them were decisively identified. Finally, the content of nootkatone was determined by HPLC. In conclusion, the methods used in this study are efficient for qualitative and quantitative analysis of Alpiniae Oxyphyllae Fructus. Also, these methods can be used to control the quality of other traditional Chinese medicines. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/jssc.202100481DOI Listing
January 2022

Fine mapping of powdery mildew resistance gene MlWE74 derived from wild emmer wheat (Triticum turgidum ssp. dicoccoides) in an NBS-LRR gene cluster.

Authors:
Keyu Zhu Miaomiao Li Haibin Wu Deyun Zhang Lingli Dong Qiuhong Wu Yongxing Chen Jingzhong Xie Ping Lu Guanghao Guo Huaizhi Zhang Panpan Zhang Beibei Li Wenling Li Lei Dong Qifei Wang Jinghuan Zhu Wenli Hu Liqiao Guo Rongge Wang Chengguo Yuan Hongjie Li Zhiyong Liu Wei Hua

Theor Appl Genet 2022 Jan 10. Epub 2022 Jan 10.

Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.

Key Message: Powdery mildew resistance gene MlWE74, originated from wild emmer wheat accession G-748-M, was mapped in an NBS-LRR gene cluster of chromosome 2BS. Wheat powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is a globally devastating disease. Wild emmer wheat (Triticum turgidum var. dicoccoides) is a valuable genetic resource for improving disease resistance in common wheat. A powdery mildew resistance gene was transferred to hexaploid wheat line WE74 from wild emmer accession G-748-M. Genetic analysis revealed that the powdery mildew resistance in WE74 is controlled by a single dominant gene, herein temporarily designated MlWE74. Bulked segregant analysis (BSA) and molecular mapping delimited MlWE74 to the terminal region of chromosome 2BS flanking by markers WGGBD412 and WGGBH346 within a genetic interval of 0.25 cM and corresponding to 799.9 kb genomic region in the Zavitan reference sequence. Sequence annotation revealed two phosphoglycerate mutase-like genes, an alpha/beta-hydrolases gene, and five NBS-LRR disease resistance genes that could serve as candidates for map-based cloning of MlWE74. The geographical location analysis indicated that MlWE74 is mainly distributed in Rosh Pinna and Amirim regions, in the northern part of Israel, where environmental conditions are favorable to the occurrence of powdery mildew. Moreover, the co-segregated marker WGGBD425 is helpful in marker-assisted transfer of MlWE74 into elite cultivars.
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http://dx.doi.org/10.1007/s00122-021-04027-2DOI Listing
January 2022

Reprogramming the spleen into a functioning 'liver' in vivo.

Authors:
Chunyan Liu Lintao Wang Mengzhen Xu Yajie Sun Zhen Xing Junfeng Zhang Chunming Wang Lei Dong

Gut 2022 Jan 7. Epub 2022 Jan 7.

State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, Jiangsu, China

Objective: Liver regeneration remains one of the biggest clinical challenges. Here, we aim to transform the spleen into a liver-like organ via directly reprogramming the splenic fibroblasts into hepatocytes in vivo.

Design: In the mouse spleen, the number of fibroblasts was through silica particles (SiO) stimulation, the expanded fibroblasts were converted to hepatocytes (iHeps) by lentiviral transfection of three key transcriptional factors (Foxa3, Gata4 and Hnf1a), and the iHeps were further expanded with tumour necrosis factor-α (TNF-α) and lentivirus-mediated expression of epidermal growth factor (EGF) and hepatocyte growth factor (HGF).

Results: SiO stimulation tripled the number of activated fibroblasts. Foxa3, Gata4 and Hnf1a converted SiO-remodelled spleen fibroblasts into 2×10 functional iHeps in one spleen. TNF-α protein and lentivirus-mediated expression of EGF and HGF further enabled the total hepatocytes to expand to 8×10 per spleen. iHeps possessed hepatic functions-such as glycogen storage, lipid accumulation and drug metabolism-and performed fundamental liver functions to improve the survival rate of mice with 90% hepatectomy.

Conclusion: Direct conversion of the spleen into a liver-like organ, without cell or tissue transplantation, establishes fundamental hepatic functions in mice, suggesting its potential value for the treatment of end-stage liver diseases.
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http://dx.doi.org/10.1136/gutjnl-2021-325018DOI Listing
January 2022

Destructing biofilms by cationic dextran through phase transition.

Authors:
Yurong Li Shaocong Wang Zhen Xing Yiming Niu Zhencheng Liao Yang Lu Junni Qiu Junfeng Zhang Chunming Wang Lei Dong

Carbohydr Polym 2022 Mar 23;279:118778. Epub 2021 Oct 23.

State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, 163 Xianlin Avenue, Nanjing 210093, China. Electronic address:

Eliminating biofilms from infected tissue presents one of the most challenging issues in clinical treatment of chronic wounds. In biofilms, the extracellular polymeric substances (EPS) form gel structures by electrostatic forces between macromolecules. We hypothesized that cationic polymers could induce the gel-to-sol phase transition of the network, leading to biofilms disruptions. We first validated this assumption by using polyethyleneimine (PEI) as a model molecule, and further synthesized two cationic dextrans with high biodegradability for in vitro and in vivo evaluation. All the cationic polymers could destruct Pseudomonas aeruginosa (P. aeruginosa) biofilms. Treating biofilm with cationic dextrans significantly enhanced the bacterial antibiotic sensitivity. When tested in a biofilm-presenting mouse wound healing model, the cationic dextrans efficiently controlled infection, and accelerated the healing process. Our findings suggest that devising cationic polymers to trigger phase transition of biofilm is an effective, straightforward, and perhaps generic strategy for anti-bacterial therapies.
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http://dx.doi.org/10.1016/j.carbpol.2021.118778DOI Listing
March 2022

Compact quartz-enhanced photoacoustic sensor for ppb-level ambient NO detection by use of a high-power laser diode and a grooved tuning fork.

Authors:
Shangzhi Li Juncheng Lu Zhijin Shang Xiangbao Zeng Yupeng Yuan Hongpeng Wu Yufeng Pan Angelo Sampaolo Pietro Patimisco Vincenzo Spagnolo Lei Dong

Photoacoustics 2022 Mar 16;25:100325. Epub 2021 Dec 16.

State Key Laboratory of Quantum Optics and Quantum Optics Devices, Institute of Laser Spectroscopy, Shanxi University, Taiyuan 030006, PR China.

A compact quartz-enhanced photoacoustic sensor for ppb-level ambient NO detection is demonstrated, in which a high-power blue laser diode module with a small divergence angle was employed to take advantages of the directly proportional relationship between sensitivity and power, hence improving the detection sensitivity. In order to extend the stability time, a custom grooved quartz tuning fork with 800-μm prong spacing is employed to avoid complex signal balance and/or optical spatial filter components. The sensor performance is optimized and assessed in terms of optical coupling, power, gas flow rate, pressure, signal linearity and stability. A minimum detectable concentration (1σ) of 7.3 ppb with an averaging time of 1 s is achieved, which can be further improved to be 0.31 ppb with an averaging time of 590 s. Continuous measurements covering a five-day period are performed to demonstrate the stability and robustness of the reported NO sensor system.
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http://dx.doi.org/10.1016/j.pacs.2021.100325DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8688703PMC
March 2022

Ppb-level gas detection using on-beam quartz-enhanced photoacoustic spectroscopy based on a 28 kHz tuning fork.

Authors:
Haoyang Lin Huadan Zheng Baiyang Antonio Zhou Montano Hongpeng Wu Marilena Giglio Angelo Sampaolo Pietro Patimisco Wenguo Zhu Yongchun Zhong Lei Dong Ruifeng Kan Jianhui Yu Vincenzo Spagnolo

Photoacoustics 2022 Mar 6;25:100321. Epub 2021 Dec 6.

PolySense Lab, Dipartimento Interateneo di Fisica, University and Politecnico of Bari, CNR-IFN, Via Amendola 173, Bari 70126, Italy.

In this paper, an on-beam quartz-enhanced photoacoustic spectroscopy (QEPAS) sensor based on a custom quartz tuning fork (QTF) acting as a photoacoustic transducer, was realized and tested. The QTF is characterized by a resonance frequency of 28 kHz, ~15% lower than that of a commercially available 32.7 kHz standard QTF. One-dimensional acoustic micro resonator (AmR) was designed and optimized by using stainless-steel capillaries. The 28 kHz QTF and AmRs are assembled in on-beam QEPAS configuration. The AmR geometrical parameters have been optimized in terms of length and internal diameter. The laser beam focus position and the AmR coupling distance were also adjusted to maximize the coupling efficiency. For comparison, QEPAS on-beam configurations based on a standard QTF and on the 28 kHz QTF were compared in terms of HO and CO detection sensitivity. In order to better characterize the performance of the system, HO, CH and CO were detected for a long time and the long-term stability was analyzed by an Allan variance analysis. With the integration time of 1 s, the detection limits for HO, CH and CO are 1.2 ppm, 28.8 ppb and 2.4 ppm, respectively. The detection limits for HO, CH and CO can be further improved to 325 ppb, 10.3 ppb and 318 ppb by increasing the integration time to 521 s, 183 s and 116 s.
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http://dx.doi.org/10.1016/j.pacs.2021.100321DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8683655PMC
March 2022

Compact QEPAS humidity sensor in SF buffer gas for high-voltage gas power systems.

Authors:
Xukun Yin Lei Dong Hongpeng Wu Miao Gao Le Zhang Xueshi Zhang Lixian Liu Xiaopeng Shao Frank K Tittel

Photoacoustics 2022 Mar 4;25:100319. Epub 2021 Dec 4.

Department of Electrical and Computer Engineering, Rice University, Houston, TX 77005, USA.

In SF insulated high-voltage gas power systems, HO is the most problematic impurity which not only decreases insulation performance but also creates an acidic atmosphere that promotes corrosion. Corrosion damages electrical equipment and leads to leaks, which pose serious safety hazards to people and the environment. A QEPAS-based sensor system for the sub-ppm level HO detection in SF buffer gas was developed by use of a near-infrared commercial DFB diode laser. Since the specific physical constants of SF are strongly different from that of N or air, the resonant frequency and -factor of the bare quartz tuning fork (QTF) had changed to 32,763 Hz and 4173, respectively. The optimal vertical detection position was 1.2 mm far from the QTF opening. After the experimental optimization of acoustic micro-resonator (AmR) parameters, gas pressures, and modulation depths, a detection limit of 0.49 ppm was achieved for an averaging time of 1 s, which provided a powerful prevention tool for the safety monitoring in power systems.
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http://dx.doi.org/10.1016/j.pacs.2021.100319DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8654977PMC
March 2022

Feasibility of salvage colonoscopy by water exchange for failed air-insufflated patients: a prospective, randomized, controlled trial.

Authors:
Mo Wang Hai-Tao Shi Xin-Xing Tantai Lei Dong Shi-Yang Ma

Scand J Gastroenterol 2021 Dec 21:1-6. Epub 2021 Dec 21.

Division of Gastroenterology, The Second Affiliated Hospital, Xi'an Jiaotong University, Xi'an, China.

Background: A complete colonoscopy is crucial for screening colorectal diseases and colorectal cancer. However, a failure rate of up to 43% still exists. Several studies have indicated that the water exchange method can enhance the cecal intubation rate while reducing discomfort of the patient. Water exchange colonoscopy (WEC) might be a salvage treatment for the patients who failed from air insufflation colonoscopy (AIC). We aimed to assess the feasibility of WEC as a salvage measure following the failure of conventional AIC.

Methods: Patients willing to undergo unsedated colonoscopy at a tertiary-care referral center in China were randomly assigned 1:1 to WEC or AIC group for salvage after the initial AIC attempt failed. Patients were blinded to group assignment. The primary outcome was cecal intubation rate, the secondary outcomes included time to the cecum, maximum pain scores, and technical difficulty level.

Results: Recruited 104 patients were randomized to the WEC ( = 52) or AIC ( = 52) group. WEC significantly increased the cecal intubation rate (92.3% vs 73.1%;  = .02). The maximum pain scores and technical difficulty level in the WEC group were significantly lower than the AIC group during salvage procedure ( < .001).

Conclusions: This randomized, controlled trial confirms that the WEC significantly enhanced cecal intubation rate in difficult colonoscopy in unsedated patients after the failure of standard AIC. The increased cecal intubation rate, lower pain scores and technical difficulty level suggest WEC is a good alternative for incomplete unsedated colonoscopy. ChiCTR2100051483.
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http://dx.doi.org/10.1080/00365521.2021.2018488DOI Listing
December 2021

The genotoxic potential of mixed nitrosamines in drinking water involves oxidative stress and Nrf2 activation.

Authors:
Lei Dong Zhiqiang Jiang Lili Yang Fen Hu Weiwei Zheng Peng Xue Songhui Jiang Melvin E Andersen Gengsheng He M James C Crabbe Weidong Qu

J Hazard Mater 2022 Mar 7;426:128010. Epub 2021 Dec 7.

Key Laboratory of the Public Health Safety, Ministry of Education, Department of Environmental Health, School of Public Health, Fudan University, Shanghai 200032, China; Center for Water and Health, School of Public Health, Fudan University, Shanghai 200032, China; Key Laboratory of Health Technology Assessment, Ministry of Health, Fudan University, Shanghai 200032, China. Electronic address:

Nitrosamine by-products in drinking water are designated as probable human carcinogens by the IARC, but the health effects of simultaneous exposure to multiple nitrosamines in drinking water remain unknown. Genotoxicity assays were used to assess the effects of both individual and mixed nitrosamines in finished drinking water produced by a large water treatment plant in Shanghai, China. Cytotoxicity and genotoxicity were measured at 1, 10-, 100- and 1000-fold actual concentrations by the Ames test, Comet assay, γ-H2AX assay, and the cytokinesis-block micronuclei assay; oxidative stress and the Nrf2 pathway were also assessed. Nitrosamines detected in drinking water included NDMA (36.45 ng/L), NDPA (44.68 ng/L), and NEMA (37.27 ng/L). Treatment with a mixture of the three nitrosamines at 1000-fold actual drinking-water concentration induced a doubling of revertants in Salmonella typhimurium strain TA100, DNA and chromosome damage in HepG2 cells, while 1-1000-fold concentrations of compounds applied singly lacked these effects. Treatment with 100- and 1000-fold concentrations increased ROS, GSH, and MDA and decreased SOD activity. Thus, nitrosamine mixtures showed greater genotoxic potential than that of the individual compounds. N-Acetylcysteine protected against the nitrosamine-induced chromosome damage, and Nrf2 pathway activation suggested that oxidative stress played pivotal roles in the genotoxic property of the nitrosamine mixtures.
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http://dx.doi.org/10.1016/j.jhazmat.2021.128010DOI Listing
March 2022

Clinical efficacy and tumour microenvironment influence of decitabine plus R-CHOP in patients with newly diagnosed diffuse large B-Cell lymphoma: Phase 1/2 and biomarker study.

Authors:
Mu-Chen Zhang Ying Fang Peng-Peng Xu Lei Dong Rong Shen Yao-Hui Huang Di Fu Zi-Xun Yan Shu Cheng Xu-Feng Jiang Qi Song Yang He Yan Zhao Min Lu Jing Ye Feng Liu Lin Cheng Chao-Fu Wang Li Wang Wei-Li Zhao

Clin Transl Med 2021 12;11(12):e584

Shanghai Institute of Hematology, State Key Laboratory of Medical Genomics, National Research Center for Translational Medicine at Shanghai, Ruijin Hospital affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China.

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http://dx.doi.org/10.1002/ctm2.584DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8684715PMC
December 2021

Smurf1 silencing restores PTEN expression that ameliorates progression of human glioblastoma and sensitizes tumor cells to mTORC1/C2 inhibitor Torin1.

Authors:
Qin Xia Wenxuan Li Sakhawat Ali Mengchuan Xu Yang Li Shengzhen Li Xinyi Meng Liqun Liu Lei Dong

iScience 2021 Dec 27;24(12):103528. Epub 2021 Nov 27.

School of Life Science, Beijing Institute of Technology, Beijing 100081, China.

Amplification of ubiquitin E3 ligase Smurf1 promotes degradation of PTEN leading to hyperactivation of the Akt/mTORC1 pathway. However, inhibitors of this pathway have not hitherto yielded promising results in clinical studies because of strong drug resistance. Here, we investigated Smurf1 expression in various glioblastoma (GB) cell lines and patient tissues. The therapeutic efficacy of Smurf1 silencing and Torin1 treatment was assessed in GB cells and orthotopic mouse model. We found Smurf1 loss elevates PTEN levels that interrupt the epidermal growth factor receptor pathway activity. Cotreatment with Smurf1 silencing and mTORC1/C2 inhibitor Torin1 remarkably decreased phosphorylation of Akt, and mTORC1 downstream targets 4EBP1 and S6K resulting in synergistic inhibitory effects. Smurf1 knockdown in orthotopic GB mouse model impaired tumor growth and enhanced cytotoxicity of Torin1. Together, these findings suggest a rational combination of Smurf1 inhibition and Torin1 as a promising new avenue to circumvent PI3K/Akt pathway-driven tumor progression and drug resistance.
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http://dx.doi.org/10.1016/j.isci.2021.103528DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8666673PMC
December 2021

miR-145-5p: A Potential Biomarker in Predicting Gleason Upgrading of Prostate Biopsy Samples Scored 3+3=6.

Authors:
Tao Wang Lei Dong Juanjuan Sun Jialiang Shao Jian Zhang Siteng Chen Chaofu Wang Gangfeng Wu Xiang Wang

Cancer Manag Res 2021 10;13:9095-9106. Epub 2021 Dec 10.

Department of Urology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.

Background: The Gleason grading system is a major tool used for prediction of prostate cancer (PCa) behavior. Because of heterogeneity and sampling errors, prognosis is variable even among patients with the same Gleason score (GS). Therefore, more accurate biomarkers that complement the Gleason system are needed to improve the clinical management of PCa.

Methods: Formalin-fixed, paraffin embedded tissue samples were obtained from radical prostatectomy (RP) (patient set 1, n=53) and needle biopsy (patient set 2, n=107; patient set 3, n=119). Cancer tissues from pure regions of each Gleason pattern (GP) were separately collected using laser-captured microdissection, followed by Real-time-PCR to determine the relative expression of miRNAs, including miR-1-5p, miR-21-5p, miR-30d-5p, miR-100-5p, miR-145-5p, miR-224-5p, and miR-708-5p. miRNA's association with Gleason upgrading (GU) was evaluated using receiver operator characteristics (ROC) curve and multivariate logistic regression analysis. The integrated miRNA targets prediction and enrichment analyses were performed to determine the potential functions of miRNA.

Results: It was found that miR-145-5p in GP3 from radical prostatectomy (RP) were overexpressed in patients with GS6 PCa compared with GS7 patients, which was further confirmed in a larger biopsy cohort. ROC curve analysis revealed that miR-145-5p in biopsy was significantly associated with GU upon RP. In multivariate analyses, miR-145-5p was an independent predictor of GU.

Conclusion: Our study indicated that differential expression of miRNAs existed in GP3 from pure GS6 and GS7 PCa, highlighting a path toward the clinical use of miRNAs in predicting GU and assisting in treatment modality selection.
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http://dx.doi.org/10.2147/CMAR.S336671DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8671722PMC
December 2021

Deep learning for automatic target volume segmentation in radiation therapy: a review.

Authors:
Hui Lin Haonan Xiao Lei Dong Kevin Boon-Keng Teo Wei Zou Jing Cai Taoran Li

Quant Imaging Med Surg 2021 Dec;11(12):4847-4858

Department of Radaition Oncology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.

Deep learning, a new branch of machine learning algorithm, has emerged as a fast growing trend in medical imaging and become the state-of-the-art method in various clinical applications such as Radiology, Histo-pathology and Radiation Oncology. Specifically in radiation oncology, deep learning has shown its power in performing automatic segmentation tasks in radiation therapy for Organs-At-Risks (OAR), given its potential in improving the efficiency of OAR contouring and reducing the inter- and intra-observer variabilities. The similar interests were shared for target volume segmentation, an essential step of radiation therapy treatment planning, where the gross tumor volume is defined and microscopic spread is encompassed. The deep learning-based automatic segmentation method has recently been expanded into target volume automatic segmentation. In this paper, the authors summarized the major deep learning architectures of supervised learning fashion related to target volume segmentation, reviewed the mechanism of each infrastructure, surveyed the use of these models in various imaging domains (including Computational Tomography with and without contrast, Magnetic Resonant Imaging and Positron Emission Tomography) and multiple clinical sites, and compared the performance of different models using standard geometric evaluation metrics. The paper concluded with a discussion of open challenges and potential paths of future research in target volume automatic segmentation and how it may benefit the clinical practice.
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http://dx.doi.org/10.21037/qims-21-168DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8611469PMC
December 2021

Rotational Speed Measurement Based on Wireless Sensors.

Authors:
Yi Zhou Lei Dong Chi Zhang Lifeng Wang Qingan Huang

Sensors (Basel) 2021 Dec 2;21(23). Epub 2021 Dec 2.

Key Laboratory of MEMS of the Ministry of Education, Southeast University, Nanjing 210096, China.

This article presents a method for detecting rotational speed by (inductor-capacitor) wireless sensors. The sensing system consists of two identical resonant tanks. One is mounted on the rotating part and the other, as a readout circuit, is placed right above the rotating part. When the inductor on the rotating part is coaxially aligned with the readout inductor during rotation, the mutual coupling between them reaches the maximum, resulting in a peak amplitude induced at the readout tank. The period of the readout signal corresponds to the rotation speed. ADS (Advanced Design System) software was used to simulate and optimize the sensing system. A synchronous detection circuit was designed. The rotational speed of an electric was measured to validate this method experimentally, and the results indicated that the maximum error of the rotation speed from 16 rps to 41 rps was 0.279 rps.
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http://dx.doi.org/10.3390/s21238055DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8659841PMC
December 2021

Sabulicella rubraurantiaca gen. nov., sp. nov., a new member of the family Acetobacteraceae, isolated from desert soil.

Authors:
Zhi-Liang Lin Shuai Li Qi-Chuang Wei Wen-Hui Lian Chun-Yan Lu Lu Xu Li-Nan Huang Lei Dong Wen-Jun Li

Arch Microbiol 2021 Dec 4;204(1). Epub 2021 Dec 4.

State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, People's Republic of China.

Strain SYSU D01096 was isolated from a sandy soil sample collected from Gurbantunggut Desert in Xinjiang, PR China. Phylogenetic analysis of the nearly full-length 16S rRNA gene sequence revealed that strain SYSU D01096 belonged to the family Acetobacteraceae and was closest to Rubritepida flocculans DSM 14296 (96.0% similarity). Cells of strain SYSU D01096 were observed to be non-motile, short rod-shaped and Gram-staining negative. The colonies were observed to be translucent, reddish orange, circular, convex and smooth. Growth occurred at 15-37 °C (optimum, 28-30 °C), pH 4.0-8.0 (optimum, pH 7.0) and 0-0.5% NaCl (w/v; optimum, 0%) on Reasoner's 2A medium. The predominant ubiquinone was identified as ubiquinone 9 and the major fatty acids were Summed Feature 8 (C ω7c and/or C ω6c) and C. The polar lipids consisted of diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), phosphatidylcholine (PC), phosphatidylglycerol (PG), one unidentified phospholipid (PL), three unidentified aminolipids (AL1-3) and one unidentified aminophospholipid (APL). The genomic DNA G + C content was 69.1%. Phylogenetic tree based on 16S rRNA gene sequences indicated strain SYSU D01096 represented an individual lineage in the family Acetobacteraceae, which was supported by 30 core gene-based phylogenomic tree. Based on the multi-analysis including physiological, chemotaxonomic and phylogenetic comparison, strain SYSU D01096 was proposed to represent a novel species of a novel genus, named Sabulicella rubraurantiaca gen. nov., sp. nov., within the family Acetobacteraceae. The type strain is SYSU D01096 (= CGMCC 1.8619 = KCTC 82268 = MCCC 1K04998).
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http://dx.doi.org/10.1007/s00203-021-02604-zDOI Listing
December 2021

Impact of Cumulative Fluid Balance During Continuous Renal Replacement Therapy on Mortality in Patients With Septic Acute Kidney Injury: A Retrospective Cohort Study.

Authors:
Jin Lin Hai Zhou Zhuang De Yuan Zhi Zhili Qi Jing Bai Lei Dong Shuai Liu Meili Duan

Front Med (Lausanne) 2021 15;8:762112. Epub 2021 Nov 15.

Department of Critical Care Medicine, Beijing Friendship Hospital, Capital Medical University, Beijing, China.

The clinicians often use continuous renal replacement therapy (CRRT) for the fluid management of patients with septic acute kidney injury (AKI). However, there is limited knowledge of the effects of changes in fluid balance (FB) on CRRT and its association with outcomes in patients with septic AKI. This study aimed to determine the association of cumulative FB (CFB) during treatment with 28-day all-cause mortality in the patients with septic AKI who require CRRT. This retrospective observational study examined patients who received CRRT due to septic AKI in a mixed intensive care unit (ICU) of a tertiary teaching hospital between January 2015 and December 2018. The patients were divided into three groups-negative FB, even FB, and positive FB-based on the CFB during CRRT. The primary outcome was 28-day all-cause mortality. We examined 227 eligible patients and the mean age was 62.4 ± 18.3 years. The even FB group had a significantly lower 28-day mortality (43.0%, = 0.007) than the positive FB group (72.7%) and the negative FB group (54.8%). The unadjusted and adjusted Cox regression models indicated that the positive FB group had an increased risk for 28-day all-cause mortality relative to the even FB group. A restricted cubic splines model indicated a J-shaped association between the CFB and 28-day all-cause mortality in the unadjusted model. Among the critically ill patients with septic AKI who require CRRT, those with positive FB had a higher mortality rate than those with even FB.
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http://dx.doi.org/10.3389/fmed.2021.762112DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8636134PMC
November 2021

Effects of SDF-1/CXCR7 on the Migration, Invasion and Epithelial-Mesenchymal Transition of Gastric Cancer Cells.

Authors:
Ameng Shi Ting Wang Miao Jia Lei Dong Haitao Shi

Front Genet 2021 9;12:760048. Epub 2021 Nov 9.

Department of Gastroenterology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.

We found that SDF-1/CXCR7 axis played an important role in the growth and proliferation of gastric cancer in the previous studies. The objectives of this study were to explore the effects of SDF-1/CXCR7 on the metastatic ability of gastric cancer cells and the possible mechanisms. CXCR7 expression in SGC-7901 gastric cancer cells was stably knocked down via lentiviral vectors. The cell migration and invasion abilities were detected by transwell migration and invasion assays. The expressions of matrix metalloproteinase 2 (MMP-2), MMP-9, vascular endothelial growth factor (VEGF), epithelial-mesenchymal transition (EMT) markers and Akt phosphorylation were detected with real-time PCR and/or western blot. We found that SDF-1 markedly enhanced the migration and invasion abilities of SGC-7901 gastric cancer cells; CXCR7 knockdown inhibited these effects. SDF-1/CXCR7 increased the expressions of MMP-2, MMP-9 and VEGF. SDF-1/CXCR7 also downregulated E-cadherin expression but upregulated N-cadherin, vimentin and Snail expressions, suggesting that SDF-1/CXCR7 could promote the development of EMT in gastric cancer cells. Furthermore, SDF-1/CXCR7 could promote Akt phosphorylation. Our results indicated that SDF-1/CXCR7 enhanced the migration, invasion and EMT of gastric cancer cells and thus CXCR7 supression may be a strategy for inhibiting gastric cancer metastasis.
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http://dx.doi.org/10.3389/fgene.2021.760048DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8630678PMC
November 2021

Hepatitis B virus-associated follicular lymphoma presents T-cell inflamed phenotype and response to lenalidomide.

Authors:
Nan Wang Wei Qin Zhong Zheng Ming Zhao Jie Xiong Hai Fang Rui Sun Yichao Wang Chen Li Lei Dong Xiaojian Sun Li Wang Pengpeng Xu Shu Cheng Haimin Xu Fan Zhang Weili Zhao

Cancer Commun (Lond) 2021 Dec 1. Epub 2021 Dec 1.

State Key Laboratory of Medical Genomics, National Research Center for Translational Medicine at Shanghai, Shanghai Institute of Hematology, Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, P. R. China.

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http://dx.doi.org/10.1002/cac2.12241DOI Listing
December 2021

C-X3-C motif chemokine ligand 1/receptor 1 regulates the M1 polarization and chemotaxis of macrophages after hypoxia/reoxygenation injury.

Authors:
Shuiming Guo Lei Dong Junhua Li Yuetao Chen Ying Yao Rui Zeng Nelli Shushakova Hermann Haller Gang Xu Song Rong

Chronic Dis Transl Med 2021 Dec 7;7(4):254-265. Epub 2021 Jun 7.

Department of Nephrology, Hannover Medical School, Hannover 30625, Germany.

Background: Macrophages play an important role in renal ischemia reperfusion injury, but the functional changes of macrophages under hypoxia/reoxygenation and the related mechanism are unclear and need to be further clarified.

Methods: The effects of hypoxia/reoxygenation on functional characteristics of RAW264.7 macrophages were analyzed through the protein expression detection of pro-inflammatory factors TNF-α and CD80, anti-inflammatory factors ARG-1 and CD206. The functional implications of C-X3-C motif chemokine receptor 1(CX3CR1) down-regulation in hypoxic macrophages were explored using small interfering RNA technology. Significance was assessed by the parametric -test or nonparametric Mann-Whitney test for two group comparisons, and a one-way ANOVA or the Kruskal-Wallis test for multiple group comparisons.

Results: Hypoxia/reoxygenation significantly increased the protein expression of M1-related pro-inflammatory factors TNF-α, CD80 and chemokine C-X3-C motif chemokine ligand 1 (CX3CL1)/CX3CR1 and inhibited the protein expression of M2-related anti-inflammatory factors ARG-1 and CD206 in a time-dependent manner in RAW264.7 cells. However, the silencing of CX3CR1 in RAW264.7 cells using specific CX3CR1-siRNA, significantly attenuated the increase in protein expression of TNF-α ( 0.05) and CD80 ( 0.01) and the inhibition of ARG-1 ( 0.01) and CD206 ( 0.01) induced by hypoxia/reoxygenation. In addition, we also found that hypoxia/reoxygenation could significantly enhance the migration (2.2-fold,  0.01) and adhesion capacity (1.5-fold,  0.01) of RAW264.7 macrophages compared with the control group, and CX3CR1-siRNA had an inhibitory role (40% and 20% reduction, respectively). For elucidating the mechanism, we showed that the phosphorylation levels of ERK ( 0.01) and the p65 subunit of NF-κB ( 0.01) of the RAW264.7 cells in the hypoxic/reoxygenation group were significantly increased, which could be attenuated by down-regulation of CX3CR1 expression ( 0.01, both). ERK inhibitors also significantly blocked the effects of hypoxic/reoxygenation on the protein expression of M1-related pro-inflammatory factors TNF-α, CD80 and M2-related anti-inflammatory factors ARG-1 and CD206. Moreover, we found that conditioned medium from polarized M1 macrophages induced by hypoxia/reoxygenation, notably increased the degree of apoptosis of hypoxia/reoxygenation-induced TCMK-1 cells, and promoted the protein expression of pro-apoptotic proteins bax ( 0.01) and cleaved-caspase 3 ( 0.01) and inhibited the expression of anti-apoptotic protein bcl-2 ( 0.01), but silencing CX3CR1 in macrophages had a protective role. Finally, we also found that the secretion of soluble CX3CL1 in RAW264.7 macrophages under hypoxia/reoxygenation was significantly increased.

Conclusions: The findings suggest that hypoxia/reoxygenation could promote M1 polarization, cell migration, and adhesion of macrophages, and that polarized macrophages induce further apoptosis of hypoxic renal tubular epithelial cells by regulating of CX3CL1/CX3CR1 signaling pathway.
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http://dx.doi.org/10.1016/j.cdtm.2021.05.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8579018PMC
December 2021

Deinococcus aestuarii sp. nov. and Deinococcus aquaedulcis sp. nov., two novel resistant bacteria isolated from pearl river estuary.

Authors:
Ling-Zi Yin Jia-Ling Li Ze-Tao Liu Bao-Zhu Fang Pandeng Wang Xiao-Qing Luo Lei Dong Li Duan Shan-Hui Li Wen-Jun Li

Antonie Van Leeuwenhoek 2022 Jan 10;115(1):59-68. Epub 2021 Nov 10.

State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, People's Republic of China.

Two novel species of the genus Deinococcus, designated SYSU M49105 and SYSU M42101, were isolated from freshwater samples of the Pearl River estuary in Guangdong, China. Phylogenetic analysis using 16S rRNA gene sequence indicated that strains SYSU M49105 and SYSU M42101 showed the highest sequence similarities to Deinococcus aetherius JCM 11751 (93.6%) and Deinococcus multiflagellatus NBRC 112888 (97.3%), respectively. Cells of both strains were Gram-staining positive, aerobic, coccus-shaped, oxidase-negative and non-motile. The cell wall contained meso-diaminopimelic acid as their diagnostic diamino acid. MK-8 was the predominant respiratory quinone for both strains. The polar lipid profile of SYSU M49105 contained two unidentified phosphoglycolipids, nine unidentified glycolipids, and five unidentified polar lipids. SYSU M42101 had one unidentified phosphoglycolipid, nine unidentified glycolipids, one unidentified aminophospholipid and four unidentified polar lipids. The major fatty acids of strains SYSU M49105 and SYSU M42101 were summed feature 3 (C ω7c and/ or C ω6c) and C The G + C contents of the novel isolates based on genomic DNAs were 69.6% and 67.4%, respectively. On the basis of phenotypic, genotypic and phylogenetic data, strains SYSU M49105 and SYSU M42101 should be considered to represent two novel species in the genus Deinococcus, for which the names Deinococcus aestuarii sp. nov. and Deinococcus aquaedulcis sp. nov. were proposed with the type strains SYSU M49105 (= KCTC 43258 = CGMCC 1.18609) and SYSU M42101 (= KCTC 43257 = CGMCC 1.18614), respectively.
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http://dx.doi.org/10.1007/s10482-021-01680-xDOI Listing
January 2022

A phase-transfer catalyst-based nanoreactor for accelerated hydrogen sulfide bio-imaging.

Authors:
Panfei Xing Yiming Niu Jiacheng Li Daping Xie Huiqun Zhou Jiaxi Chen Lei Dong Chunming Wang

Nanoscale 2021 Nov 25;13(45):19049-19055. Epub 2021 Nov 25.

State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Taipa, Macau SAR, China.

Hydrogen sulfide (HS) is an important signaling molecule in various biological processes; however, its real-time monitoring in living cells is hampered by long detection time for current fluorescent probes. To overcome this challenge, we designed a phase-transfer catalyst (PTC) approach to accelerate the reaction between the probe and the analyte by conjugating common fluorescent probes - mostly hydrophobic small molecules - with an amphiphilic PEG-PPG-PEG polymer, enabling the controllable assembly of HS nanoprobes in an aqueous solution. The PEG block helps to establish a PTC microenvironment that endows the assembled nanoprobes with a significantly reduced detection time (3-10 min; 20-60 min for small-molecule probes). Based on this approach, we synthesised two nanoprobes of different wavelengths, and , which can sensitively detect HS in living macrophage cells with bright fluorescence starting at as early as 7 min and reaching stability at 15 min. These data suggest PTC-based nanoprobes as a new and generic approach for constructing sensitive fluorescent probes for the real-time imaging of HS, and perhaps other molecules in future, under biological conditions.
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http://dx.doi.org/10.1039/d1nr04931cDOI Listing
November 2021

Simultaneous Multiple Liver Metastasis Treated with Pencil Beam Proton Stereotactic Body Radiotherapy (SBRT).

Authors:
Neil K Taunk Brendan Burgdorf Lei Dong Edgar Ben-Josef

Int J Part Ther 2021 22;8(2):89-94. Epub 2021 Apr 22.

Department of Radiation Oncology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.

Compared with photon stereotactic body radiotherapy (SBRT) plans that may have to use many more penetrating x-ray beams for each isocenter, proton SBRT with ultrahypofractionated doses use fewer beam angles and offer significantly reduced low-dose radiation bath to normal liver tissue. We demonstrate techniques to deliver safe and effective proton SBRT, where planning and organ motion complexity further increased with multiple liver lesions. For treatment planning, we recommend robust and logical beam angles, avoiding devices and encouraging entry perpendicular to the dominant motion, as well as volumetric repainting to mitigate the interplay effect to clinically acceptable levels. This report highlights the significant technical challenges with ultrahypofractionated proton pencil beam scanning liver therapy, how they are managed, and the effectiveness of this treatment.
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http://dx.doi.org/10.14338/IJPT-20-00085.1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8489493PMC
April 2021

Jolkinolide B attenuates laryngeal cancer cell growth and induces apoptosis via PTEN/PI3K/Akt signaling pathway.

Authors:
Lei Dong Feifei Liu Dawei Liu Shasha Kang Xin Yang Junxia Wang

In Vitro Cell Dev Biol Anim 2021 Sep 25;57(8):786-794. Epub 2021 Oct 25.

Department of Otorhinolaryngology, Yantai Haigang Hospital, No. 100 Xingfu Road, Zhifu District, Yantai, 264000, Shandong, China.

Jolkinolide B (JB) is a bioactive diterpenoid, isolated from the root of Euphorbia fischeriana Steud, and has been reported to have anti-tumor and anti-inflammation function by regulation of cell migration, invasion, apoptosis, and cell cycle. We aimed to evaluate the effect of JB on laryngeal cancer cells. Human normal larynx epithelial (HBE) cells and cancer cell lines TU212, TU177, and Hep-2 were cultured; MTT assay was used to assess cell proliferation. LY294002 (a PI3K/Akt inhibitor) and IGF-1 (a PI3K/Akt activator) were employed to investigate the expression of PI3K/Akt pathway. Cell migration and invasion activities were detected by scratch wound healing and transwell assay, respectively. Flow cytometry assay was used to assess cell apoptosis. The expression levels of proteins were assessed by immunofluorescence and Western blotting assay. JB inhibited TU212, TU177, and Hep-2 cell viability with an IC value of 54.57 ± 0.53 μg/mL, 44.82 ± 0.32 μg/mL, and 49.63 ± 0.47 μg/mL, respectively. Compared with control group, the proliferation, migration, and invasion of cells significantly decreased after JB and LY294002 treatment, while cell apoptosis increased. In IGF-1 group, the results were opposite compared to the JB and LY294002 groups. Western blotting results showed that JB and LY294002 treatment significantly inhibited the levels of Bcl-2, p-PI3K, and p-Akt while the levels of Bax, cleaved caspase-3, and PTEN protein significantly increased. Our study suggested that JB exhibits an inhibition effect on laryngeal cancer cell growth in vitro.
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http://dx.doi.org/10.1007/s11626-021-00612-3DOI Listing
September 2021

Corrigendum to "Effect of therapeutic administration of β-lactam antibiotics on the bacterial community and antibiotic resistance patterns in milk" (J. Dairy Sci. 104:7018-7025).

Authors:
Lei Dong Lu Meng Huimin Liu Haoming Wu Haiyan Hu Nan Zheng Jiaqi Wang Martine Schroyen

J Dairy Sci 2021 Nov;104(11):12185

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http://dx.doi.org/10.3168/jds.2021-104-11-12185DOI Listing
November 2021

Prevalence, Antimicrobial Susceptibility, and Molecular Characterization of Isolated From Raw Milk in Dairy Herds in Northern China.

Authors:
Huimin Liu Lu Meng Lei Dong Yangdong Zhang Jiaqi Wang Nan Zheng

Front Microbiol 2021 24;12:730656. Epub 2021 Sep 24.

Laboratory of Quality and Safety Risk Assessment for Dairy Products of Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.

is a common bacterium in the intestines of animals, and it is also the major important cause of toxic mastitis, which is an acute or peracute disease that causes a higher incidence of death and culling of cattle. The purpose of this study was to investigate strains isolated from the raw milk of dairy cattle in Northern China, and the antibacterial susceptibility of these strains and essential virulence genes. From May to September 2015, 195 raw milk samples were collected from 195 dairy farms located in Northern China. Among the samples, 67 (34.4%) samples were positive for . About 67 strains were isolated from these 67 samples. The prevalence of Shiga toxin-producing (STEC), enterotoxigenic (ETEC), enteropathogenic (EPEC), and enteroinvasive (EIEC) were 9, 6, 4.5, and 1.5%, respectively. Among the virulence genes detected, 1 was the most prevalent (6/67, 9%) gene, followed by (3/67, 4.5%), and B (2/67, 3%). Moreover, the strains exhibited different resistance levels to ampicillin (46.3%), amoxicillin-clavulanic acid (16.4%), trimethoprim-sulfamethoxazole (13.4%), tetracycline (13.4%), cefoxitin (11.9%), chloramphenicol (7.5%), kanamycin (7.5%), streptomycin (6.0%), tobramycin (4.5%), azithromycin (4.5%), and ciprofloxacin (1.5%). All of the isolates were susceptible to gentamicin. The prevalence of β-lactamase-encoding genes was 34.3% in 67 isolates and 45% in 40 β-lactam-resistance isolates. The overall prevalence of , , , and genes were 1.5, 20.9, 10.4, and 1.5%, respectively. Nine non-pathogenic isolates also carried β-lactamase resistance genes, which may transfer to other pathogenic and pose a threat to the farm's mastitis management projects. Our results showed that most of were multidrug resistant and possessed multiple virulence genes, which may have a huge potential hazard with public health, and antibiotic resistance of was prevalent in dairy herds in Northern China, and ampicillin should be used cautiously for mastitis caused by in Northern China.
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http://dx.doi.org/10.3389/fmicb.2021.730656DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8500479PMC
September 2021

Recurrence of Primary Aldosteronism 10 Years After Left Adrenalectomy for Aldosterone-Producing Adenoma: A Case Report.

Authors:
Linghui Kong Jin Zhang Lei Dong Jianzhong Xu Ping-Jin Gao Ji-Guang Wang Limin Zhu

Front Endocrinol (Lausanne) 2021 24;12:728595. Epub 2021 Sep 24.

Department of Cardiovascular Medicine, Ruijin Hospital, Shanghai Institute of Hypertension, Shanghai Jiaotong University School of Medicine, Shanghai, China.

A 29-year-old female patient diagnosed with primary aldosteronism (PA) in 2004 underwent complete adrenalectomy for left aldosterone-producing adenoma (APA) confirmed by hematoxylin and eosin (HE) and CYP11B2 staining. Her hypokalemia was corrected, and her blood pressure (BP) normalized and maintained without medication for 10 years. In 2014, her BP became elevated again, and a recurrence of PA with an adenoma on the right adrenal gland was discovered by computed tomography scan. She underwent partial right adrenalectomy in 2018 due to unsatisfactory BP control with medication and gradually enlarging adenoma. The resected adrenal tissue contained a CYP11B2 staining positive APA. Her BP was then controlled by two drugs. Sanger sequencing of DNA extracted from tissue slices revealed that both left and right adenomas carried the same aldosterone-driver gene mutation, but with different nucleotide changes. We suggest that patients who undergo adrenalectomy for APA should be followed up for life.
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http://dx.doi.org/10.3389/fendo.2021.728595DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8498213PMC
September 2021

sp. nov., a novel bacterium isolated from Pearl River estuary.

Authors:
Ling-Zi Yin Jia-Ling Li Bao-Zhu Fang Ze-Tao Liu Pandeng Wang Lei Dong Li Duan Xiao-Qing Luo Shan-Hui Li Wen-Jun Li

Int J Syst Evol Microbiol 2021 Oct;71(10)

State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, Guangdong, PR China.

A novel species of the genus , designated SYSU M41301, was isolated from water sample of the Pearl River estuary in Guangdong, China. Polyphasic, taxonomic and phylogenomic analyses were used to determine the taxonomy position of the strain. Phylogenetic analysis using 16S rRNA gene sequence indicated that strain SYSU M41301 showed the highest sequence similarity to KCTC 22213 (97.9 %) and KCTC 23339 (96.4 %). The novel species could be differentiated from other species of the genus by its distinct phenotypic and genotypic characteristics. The isolate was Gram-staining-negative, aerobic, short rod-shape, oxidase-positive and non-motile. The predominant respiratory quinone was ubiquinone 8 (Q-8). The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, and one unidentified polar lipid. The major fatty acids (>10 % of total) were 11-methyl C 7, summed feature 3 (C c / or C c) and summed feature 8 (C 7 and/or C 6). The G+C content of the novel isolate based on genomic DNA was 72.0 mol%. On the basis of phenotypic, genotypic and phylogenetic data, strain SYSU M41301 should be considered to represent a novel species in the genus for which the name sp. nov. is proposed with the type strain SYSU M41301 (=KCTC 72726=CGMCC 1.18613).
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http://dx.doi.org/10.1099/ijsem.0.004994DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8604164PMC
October 2021
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