Publications by authors named "Lei Cui"

538 Publications

Risk Factors and Prognosis of New-Onset Chronic Kidney Disease Following Orthotopic Liver Transplantation: A Retrospective Case-Control Study.

Med Sci Monit 2021 Sep 19;27:e931834. Epub 2021 Sep 19.

Department of Anesthesiology, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua University, Beijing, China (mainland).

BACKGROUND We have undertaken this investigation to explore the perioperative risk factors of new-onset chronic kidney disease (NOCKD) after orthotopic liver transplantation (OLT), and to provide an early prediction model for the screening of NOCKD high-risk populations. MATERIAL AND METHODS A retrospective case-control study was performed in adult recipients who received OLT in our center between January 2018 and January 2020. Perioperative data were collected using the center's electronic medical record system. Logistics regression analysis was used to determine risk factors for NOCKD within 1 year following OLT. Kaplan-Meier and log-rank tests were used to evaluate the 1-year survival of recipients with NOCKD or without NOCKD. RESULTS A total of 174 patients were included in this study, and 29 patients developed NOCKD after OLT. Logistic multivariate regression analysis showed that preoperative diabetes, high model for end-stage liver disease (MELD) score, postoperative acute kidney injury (AKI), and postoperative renal replacement therapy (RRT) were independent risk factors for NOCKD 1 year after OLT. The 1-year survival rate of NOCKD recipients waas significantly lower than that of patients who did not receive NOCKD. CONCLUSIONS Diabetes mellitus, MELD score, postoperative AKI, and requirement for postoperative RRT are independent risk factors for NOCKD after OLT, which may have great potential for personalized decision making and predicting the 1-year postoperative mortality of the recipient.
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http://dx.doi.org/10.12659/MSM.931834DOI Listing
September 2021

EXPRESS: Predictability effects and parafoveal processing of compound words in natural Chinese reading.

Q J Exp Psychol (Hove) 2021 Sep 10:17470218211048193. Epub 2021 Sep 10.

University of Central Lancashire, UK 6723.

We report a boundary paradigm eye movement experiment to investigate whether the predictability of the second character of a two-character compound word affects how it is processed prior to direct fixation during reading. The boundary was positioned immediately prior to the second character of the target word, which itself was either predictable or unpredictable. The preview was either a pseudocharacter (nonsense preview), or an identity preview. We obtained clear preview effects in all conditions, but more importantly, skipping probability for the second character of the target word and the whole target word from pretarget was greater when it was predictable than when it was not predictable from the preceding context. Interactive effects for later measures on the whole target word (gaze duration and go-past time) were also obtained. These results demonstrate that predictability information from preceding sentential context and information regarding the likely identity of upcoming characters are used concurrently to constrain the nature of lexical processing during natural Chinese reading.
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http://dx.doi.org/10.1177/17470218211048193DOI Listing
September 2021

Efficacy and safety of autologous platelet-rich fibrin for the treatment of infertility with intrauterine adhesions.

J Obstet Gynaecol Res 2021 Sep 5. Epub 2021 Sep 5.

Department of Obstetrics and Gynecology, Beijing Shijitan Hospital, Capital Medical University, Beijing, China.

Aim: To evaluate the efficacy and safety of autologous platelet-rich fibrin (PRF) for the treatment of infertility with intrauterine adhesions.

Methods: Forty patients who suffered from infertility with intrauterine adhesions (IUAs) were enrolled in this prospective trial from October 2018 to December 2019. They were randomly divided into a PRF group and a control group. Both groups underwent transcervical resection of adhesions (TCRA) during the follicular phase. PRF group: PRF prepared from autologous venous blood was placed in the uterine cavity after TCRA and after the first menstrual re-fluid; control group: no PRF insertion. After the second menstrual re-fluid, both groups underwent hysteroscopy to reevaluate adhesion severity. Pregnancy rate, intrauterine adhesion score, menstrual volume and duration, and endometrial thickness were assessed and adverse events were recorded.

Results: (1) PRF group pregnancy rate was significantly higher than the control group (55.0% vs. 20.0%). Mean follow-up time was (17.63 ± 2.33) months. All patients fell pregnant naturally. Four (PRF) and one (control) patients delivered live births without placental complications. (2) Intrauterine adhesion scores decreased compared with treatment-before. (3) In the pictorial blood loss assessment chart, score and menstrual duration during the 3rd menstrual cycle showed significant improvements for both groups. (4) Endometrial thickness also showed an increasing trend, and subendometrial bloodflow improved. (5) No treatment-related serious adverse events were noted.

Conclusion: Placing PRF into the uterine cavity of infertility patients with IUAs following TCRA is a safe and effective therapeutic method.
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http://dx.doi.org/10.1111/jog.14964DOI Listing
September 2021

The use of free myocutaneous flap and implant reinsertion for staged cranial reconstruction in patients with titanium mesh exposure and large skull defects with soft tissue infection after cranioplasty: Report of 19 cases.

Microsurgery 2021 Sep 2. Epub 2021 Sep 2.

Department of Plastic and Reconstructive Surgery, First Medical Center of PLA General Hospital, Beijing, China.

Background: Management of cranial defects following failed cranioplasty due to titanium mesh exposure and infection is challenging. The purpose of this report is to describe a modified technique using a free myocutaneous flap transfer for primary soft tissue reconstruction, and titanium mesh reinsertion for cranioplasty revision.

Methods: Nineteen patients with titanium mesh exposure and infection following cranioplasty were treated from January 2012 to January 2019. The average patient age was 41.89 years and the average size of the cranial defect was 7.74 × 13.92 cm. The reasons for craniotomy were craniocerebral trauma (n = 17), cerebrovascular disease (n = 1), and brain tumor (n = 1). The mean duration between implant exposure and current procedure was 7.16 months. Implant was removed and a free myocutaneous flap was designed to cover both scalp and cranium defects. After a mean duration of 12.32 months, implants were re-inserted in a vascularized pocket at the second stage by elevating a plane between the previously transferred fascia layer and muscle layer.

Results: The average sizes of the muscle flaps and skin paddles were 7.74 × 13.92 cm and 4.97 × 8.97 cm. The average size of the implants was 8.24 × 14.42 cm. All flaps survived completely with no complication. After an average follow-up of 48.16 months there were no cranioplasty failures. Functional coverage of craniectomy defect sites with normalized head contour was achieved.

Conclusions: The use of free myocutaneous flap and implant reinsertion achieved durable cranial and scalp defect reconstruction and aesthetic outcomes. The myocutaneous flap increases blood supply to the scalp, which may reduce the chances of infection and implant re-exposure.
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http://dx.doi.org/10.1002/micr.30800DOI Listing
September 2021

Tough, Resilient, Adhesive, and Anti-Freezing Hydrogels Cross-Linked with a Macromolecular Cross-Linker for Wearable Strain Sensors.

ACS Appl Mater Interfaces 2021 Sep 26;13(35):42052-42062. Epub 2021 Aug 26.

Key Laboratory of Functional Polymer Materials and State Key Laboratory of Medicinal Chemical Biology, Institute of Polymer Chemistry, College of Chemistry, Nankai University, Tianjin 300071, China.

Ideal conductive hydrogels for flexible, wearable strain sensors should be tough, highly resilient, adhesive, and anti-freezing. However, such hydrogels are difficult to design. Herein, a multifunctional macromolecular cross-linker (MC) based on poly(hydroxyethyl-l-glutamine) was designed and used to synthesize the hydrogels. Cross-linking with the MC leads to a reduced inhomogeneity of the gel network. Therefore, the mechanical properties of the gels are significantly improved compared with the ordinary hydrogels cross-linked with the conventional cross-linker ,-methylenebisacrylamide (BIS). The MC-cross-linked gels also exhibit high resilience. At the same time, replacing BIS with MC significantly improves the adhesive properties of the gel, which is attributed to the introduction of a large amount of adhesive groups with the MC. The gels can stick to various substrates including skin. The good tissue adhesiveness of the gel allows it to stick to skin by itself without using any straps or adhesive tapes when used as a flexible wearable strain sensor. Both large and subtle human movements were successfully monitored using the sensor. The signals are highly stable and reliable, thanks to the high resilience of the gel. The introduction of the polar groups also improved dramatically the anti-freezing properties of the gels. Even at -20 °C, the gels still remained highly flexible and stretchable, therefore allowing the gel-based sensor to work at sub-zero temperatures. The excellent toughness, resilience, tissue-adhesiveness, and anti-freezing properties of the gel make it a good choice for a flexible wearable sensor.
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http://dx.doi.org/10.1021/acsami.1c12687DOI Listing
September 2021

Coordination Polymer-Derived FeN Nanoparticles for Efficient Electrocatalytic Oxygen Evolution.

Inorg Chem 2021 Aug 26;60(16):12136-12150. Epub 2021 Jul 26.

Department of Applied Chemistry, College of Chemistry and Chemical Engineering, Chongqing University, Chongqing 401331, P. R. China.

Based on a coordination polymer, FeCl(4,4'-bpy) (4,4'-bpy = 4,4'-bipyridine) and the carbon nanotube (CNT)/NaCl dual template, FeN nanoparticles (NPs) were synthesized via chemical thermolysis in the absence of an extra nitrogen source. The decomposition of 4,4'-bpy under high temperature produces thin carbon coating for FeN NPs. Also, the CNT template anchors the FeN NPs to avoid aggregation. The sample (denoted as FeN-C N) exhibits excellent electrocatalytic oxygen evolution reaction (OER) behavior even with a small molar ratio of FeN (Fe: 4.9 at. %), which can deliver a current density of 10 mA cm at an overpotential of 218 mV with a Tafel slope of 84 mV dec and long-term OER activity during 60 h electrolysis at 20 mA cm. Furthermore, the sample after 20 h electrolysis, denoted as Post-FeN-C N (20 h), displays enhanced OER activity with a smaller Tafel slope of 41 mV dec and overpotentials of 195 and 327 mV at 10 and 100 mA cm, respectively, which is mainly due to the partial transformation of FeN into FeOOH. The OER mechanism is investigated by density functional theory calculations, and it is found that the surface partial oxidation of FeN leads to the effective OER electrolysis, which changes the electron density of the superficial atoms and induces the moderate adsorption for the intermediates.
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http://dx.doi.org/10.1021/acs.inorgchem.1c01352DOI Listing
August 2021

Fixed-time ESO based fixed-time integral terminal sliding mode controller design for a missile.

ISA Trans 2021 Jul 13. Epub 2021 Jul 13.

College of Automation Engineering, Institute of Flight Control, Nanjing University of Aeronautics and Astronautics, Nanjing, 211106, China. Electronic address:

This paper studies a novel fixed-time extended state observer based fixed-time integral terminal sliding mode controller for partial integrated guidance and control design. Firstly, a class of arbitrary-order systems with fixed-time stability is proposed by utilizing homogeneous approach, whose upper bound of convergence time is given. Then, an arbitrary-order fixed-time integral terminal sliding mode control is designed based on the proposed arbitrary-order fixed-time stable system, which avoids the singular problem. Subsequently, this paper constructs a new fixed-time extended state observer to further actively compensate for the disturbance caused by unknown target acceleration. Finally, numerical simulations show the effectiveness of the proposed controller.
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http://dx.doi.org/10.1016/j.isatra.2021.06.039DOI Listing
July 2021

Second-line regimen for CNS-involved pediatric Langerhans cell histiocytosis.

Pituitary 2021 Jul 24. Epub 2021 Jul 24.

Beijing Key Laboratory of Pediatric Hematology Oncology, National Key Discipline of Pediatrics, Key Laboratory of Major Diseases in Children, Ministry of Education, Hematology Oncology Center, National Center for Children's Health, Beijing Children's Hospital, Capital Medical University, Beijing, 100045, China.

Purpose: Langerhans cell histiocytosis (LCH) can affect any organ. Central nervous system (CNS) involvement is rare, and its management is poorly understood. This study aimed to analyze the clinical response and prognosis of pediatric LCH with central diabetes insipidus (CDI) treated with second-line therapy with cytarabine (Ara-c), cladribine (2-cdA), dexamethasone, and vindesine.

Methods: This retrospective case series study included pediatric LCH with CDI treated at Beijing Children's Hospital affiliated with Capital Medical University (11/2012-01/2018). After the first-line 2009-LCH regimen, patients with active disease/worse response, relapse, or no significant improvement in risk organs, pituitary, or lung were given the second-line therapy. Baseline characteristics, clinical response and adverse reactions were observed.

Results: Twenty-six children with CDI and disappearance of hyperintensity in the posterior pituitary were included. They received "Regimen A" Ara-c + dexamethasone + vindesine (n = 7) or "Regimen B" Ara-c + dexamethasone + vindesine + 2-cdA (n = 19) as second-line therapy. There were 14 patients with CDI but without pituitary stalk thickening (PST) and 12 with CDI and PST. In patients with CDI alone, 4/4 patients receiving Regimen A and 3/10 receiving Regimen B improved. All patients with CDI and PST showed improvement for PST. The reappearance of hyperintensity at the posterior pituitary was observed in 10 patients with CDI. All 26 children were alive after a median follow-up of 40.5 months. There were no chemotherapy-related deaths.

Conclusion: A combined therapy with Ara-c, 2-cdA, dexamethasone, and vindesine could partially alleviate pituitary disease conditions in pediatric LCH with CNS involvement, with good tolerance.
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http://dx.doi.org/10.1007/s11102-021-01176-xDOI Listing
July 2021

Diels-Alder Cross-Linked, Washing-Free Hydrogel Films with Ordered Wrinkling Patterns for Multicellular Spheroid Generation.

Biomacromolecules 2021 08 22;22(8):3474-3485. Epub 2021 Jul 22.

Key Laboratory of Functional Polymer Materials and State Key Laboratory of Medicinal Chemical Biology, Institute of Polymer Chemistry, College of Chemistry, Nankai University, Tianjin 300071, China.

Three-dimensional (3D) multicellular spheroids are a new generation cell model, however, their applications are severely limited by difficulties in their generation. Here patterned poly(2-hydroxyethyl methacrylate) (PHEMA) hydrogel films were synthesized for their generation. Instead of polymerization of HEMA monomers in the presence of a cross-linker, here the PHEMA films were synthesized by cross-linking furan-functionalized linear PHEMA, PHEMA-furan, and maleimide-functionalized linear PHEMA, PHEMA-mal, via Diels-Alder (DA) reaction between furan and maleimide groups. A thermal treatment temperature of 75 °C was chosen for the cross-linking reaction. The occurrence of DA reaction was confirmed by IR spectra. Using this method, cross-linked PHEMA films with smooth surface were successfully synthesized in the well of cell culture plates. The films were then patterned by simply adding water to swell them. Highly ordered, honeycomb-like wrinkling patterns were successfully obtained by adjusting the furan and maleimide contents in the precursor linear polymers. The patterned hydrogel films were used to generate multicellular spheroids. Guided by the patterns, 3D spheroids with narrow size distribution, tunable size, and high cell viability were successfully obtained. The patterned PHEMA films reported here exhibited a lot of advantages. The patterning method was quite simple and required no template or special equipment. They were synthesized in commercial cell culture plates. Particularly, thanks to the clean nature of the DA reaction, no low molecular weight monomer, cross-linker, initiator, or catalyst, which were potentially cytotoxic, was involved in the film synthesis, and no byproduct was produced and left in the film. The resulting films presented a high biocompatibility, allowing the avoidance of the tedious washing step. The films synthesized here were expected to have high potential for massive production of well-defined multicellular spheroids.
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http://dx.doi.org/10.1021/acs.biomac.1c00570DOI Listing
August 2021

B -Corrected T1 Mapping in Lung Cancer: Repeatability, Reproducibility, and Identification of Histological Types.

J Magn Reson Imaging 2021 Jul 22. Epub 2021 Jul 22.

Siemens Healthineers Digital Technology Co., Ltd, Shanghai, China.

Background: T1 mapping can potentially quantitatively assess the intrinsic properties of tumors. B correction can reduce the magnetic field inhomogeneity.

Purpose: To assess the repeatability and reproducibility of B -corrected T1 mapping for lung cancer and the ability to identify pathological types.

Study Type: Prospective reproducibility study.

Population: Sixty lung cancer patients (22 with emphysema) with a total of 60 lesions (adenocarcinoma [n = 23], squamous cell carcinoma [n = 19], and small-cell lung cancer [SCLC] [n = 18]).

Field Strength/sequence: A 3 T/B -corrected 3D variable flip angle T1 mapping and free-breathing diffusion-weighted imaging.

Assessment: Intraobserver, interobserver, and test-retest reproducibility of minimum, maximum, mean, and SD of lung tumor T1 values were assessed. The correlation between mean T1 and apparent diffusion coefficient (ADC) and differences between different histological types of lung cancer were evaluated.

Statistical Tests: Intraclass correlation coefficients (ICCs), within-subject coefficients of variation (WCVs), Bland-Altman plots, Pearson's correlation coefficient (r), and analysis of variance (ANOVA). A P value <0.05 was considered to be statistically significant.

Results: No significant differences were found in minimum, maximum, mean, and SD T1 values for repeated measurements (intraobserver and interobserver) and repeated examinations (P = 0.103-0.979). All parameters showed good intraobserver, interobserver and test-retest reproducibility (ICC, 0.780-0.978), except the maximum T1 value (ICC, 0.645-0.922). The mean T1 exhibited the best reproducibility and repeatability, with an average difference <6% for repeated measurements, <8% for repeated scans in lung cancer patients, and<10% for repeated scans in those with emphysema. The mean T1 correlated moderately with ADC (r = -0.580, -0.516, and -0.511 for observers A, B, and C). Both mean T1 and mean ADC were significantly different in SCLC patients compared with those in adenocarcinoma and squamous cell carcinoma patients.

Data Conclusion: The mean T1 from B -corrected T1 mapping is a repeatable parameter with the potential to identify histological types of lung cancer and thus may be a promising imaging biomarker for characterizing lung cancer.

Evidence Level: 1 TECHNICAL EFFICACY: Stage 2.
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http://dx.doi.org/10.1002/jmri.27844DOI Listing
July 2021

Optimized cytotoxicity assay for co-suspended effector and target cells.

J Immunol Methods 2021 Oct 14;497:113100. Epub 2021 Jul 14.

Shanghai Cell Therapy Group Co., LTD, Shanghai 201805, China. Electronic address:

In recent years, adoptive cell therapy of immune effector cells, such as chimeric antigen receptor-T (CAR-T) cells, natural killer (NK) cells, and epitope-specific cytotoxic T lymphocyte (CTL) cells have been employed in clinical trials. In addition, CD19 CAR-T cells have been approved by the FDA for treatment of non-Hodgkin lymphoma and diffuse large B-cell lymphoma. In this context, it is vital to detect cellular cytotoxicity and monitor the quality of ex vivo expanded immune cells before product release and patient infusion. Target cells could proliferate in parallel with effector cells during the cytotoxicity assay, making it difficult to estimate the death ratio using conventional approaches. Meanwhile, non-specific dyes or non-homogeneous biomarkers for target cells may interfere with the final readout post addition of effector cells. Here, we modified a component of the coincubation medium to suppress the spontaneous release of bis(acetoxymethyl)2,2':6',2″-terpyridine-6,6″-dicarboxylate and sustained the window at a stable range (~70%). Further, the optimized Eu-TDA method presented reliable outcomes compared with lactate dehydrogenase detection and was compatible with cytotoxicity tests for NK cells and specific CTLs. Finally, the reported assay can accurately detect death of target cells depending on the amount of hydrophilic complex and can be reliably applied in quality control and cell activity evaluation tests on co-suspended effector and target cells. SUMMARY: A medium component for cellular coincubations (and associated protocols) have been optimized and validated for cytotoxicity assays, which can reliably evaluate the potency of engineered CD19 CAR-T cells, NK cells, and specific CTLs. In particular, the reported method can be applied widely in routine assays for bi-suspended effector and target cells with a stable window.
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http://dx.doi.org/10.1016/j.jim.2021.113100DOI Listing
October 2021

Tai Chi Chuan Alters Brain Functional Network Plasticity and Promotes Cognitive Flexibility.

Front Psychol 2021 29;12:665419. Epub 2021 Jun 29.

PE Department, Renmin University of China, Beijing, China.

This study used resting-state functional magnetic resonance imaging to investigate the effects of 8 weeks of Tai Chi Chuan and general aerobic exercise on the topological parameters of brain functional networks, explored the advantages of Tai Chi Chuan for improving functional network plasticity and cognitive flexibility, and examined how changes in topological attributes of brain functional networks relate to cognitive flexibility. Thirty-six healthy adults were grouped into Tai Chi Chuan (Bafa Wubu of Tai Chi), general aerobic exercise (brisk walking), and control groups. All of the subjects underwent fMRI and behavioral assessment before and after the exercise intervention. Tai Chi Chuan exercise significantly enhanced the clustering coefficient and local efficiency compared with general aerobic exercise. Regarding the nodal properties, Tai Chi Chuan significantly enhanced the nodal clustering coefficient of the bilateral olfactory cortex and left thalamus, significantly reduced the nodal clustering coefficient of the left inferior temporal gyrus, significantly improved the nodal efficiency of the right precuneus and bilateral posterior cingulate gyrus, and significantly improved the nodal local efficiency of the left thalamus and right olfactory cortex. Furthermore, the behavioral performance results demonstrated that cognitive flexibility was enhanced by Tai Chi Chuan. The change in the nodal clustering coefficient in the left thalamus induced by Tai Chi Chuan was a significant predictor of cognitive flexibility. These findings demonstrated that Tai Chi Chuan could promote brain functional specialization. Brain functional specialization enhanced by Tai Chi Chuan exercise was a predictor of greater cognitive flexibility.
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http://dx.doi.org/10.3389/fpsyg.2021.665419DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8275936PMC
June 2021

Mevalonate Blockade in Cancer Cells Triggers CLEC9A Dendritic Cell-Mediated Antitumor Immunity.

Cancer Res 2021 Sep 15;81(17):4514-4528. Epub 2021 Jul 15.

State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, China.

Hyperactive mevalonate (MVA) metabolic activity is often observed in cancer cells, and blockade of this pathway inhibits tumor cell lipid synthesis and cell growth and enhances tumor immunogenicity. How tumor cell MVA metabolic blockade promotes antitumor immune responses, however, remains unclear. Here we show that inhibition of the MVA metabolic pathway in tumor cells elicits type 1 classical dendritic cells (cDC1)-mediated tumor recognition and antigen cross-presentation for antitumor immunity. Mechanistically, MVA blockade disrupted prenylation of the small GTPase Rac1 and induced cancer cell actin filament exposure, which was recognized by CLEC9A, a C-lectin receptor specifically expressed on cDC1s, in turn activating antitumor T cells. MVA pathway blockade or Rac1 knockdown in tumor cells induced CD8 T-cell-mediated antitumor immunity in immunocompetent mice but not in mice lacking CLEC9A dendritic cells. These findings demonstrate tumor MVA metabolic blockade stimulates a cDC1 response through CLEC9A-mediated immune recognition of tumor cell cytoskeleton, illustrating a new immune surveillance mechanism by which dendritic cells monitor tumor metabolic dysregulation and providing insight into how MVA pathway inhibition may potentiate anticancer immunity. SIGNIFICANCE: These findings suggest that mevalonate blockade in cancer cells disrupts Rac1 prenylation to increase recognition and cross-presentation by conventional dendritic cells, suggesting this axis as a potential target for cancer immunotherapy.
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http://dx.doi.org/10.1158/0008-5472.CAN-20-3977DOI Listing
September 2021

Caecal microbiota could effectively increase chicken growth performance by regulating fat metabolism.

Microb Biotechnol 2021 Jul 15. Epub 2021 Jul 15.

Department of Basic Veterinary Medicine, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.

It has been established that gut microbiota influences chicken growth performance and fat metabolism. However, whether gut microbiota affects chicken growth performance by regulating fat metabolism remains unclear. Therefore, seven-week-old chickens with high or low body weight were used in the present study. There were significant differences in body weight, breast and leg muscle indices, and cross-sectional area of muscle cells, suggesting different growth performance. The relative abundance of gut microbiota in the caecal contents at the genus level was compared by 16S rRNA gene sequencing. The results of LEfSe indicated that high body weight chickens contained Microbacterium and Sphingomonas more abundantly (P < 0.05). In contrast, low body weight chickens contained Slackia more abundantly (P < 0.05). The results of H & E, qPCR, IHC, WB and blood analysis suggested significantly different fat metabolism level in serum, liver, abdominal adipose, breast and leg muscles between high and low body weight chickens. Spearman correlation analysis revealed that fat metabolism positively correlated with the relative abundance of Microbacterium and Sphingomonas while negatively correlated with the abundance of Slackia. Furthermore, faecal microbiota transplantation was performed, which verified that transferring faecal microbiota from adult chickens with high body weight into one-day-old chickens improved growth performance and fat metabolism in liver by remodelling the gut microbiota. Overall, these results suggested that gut microbiota could affect chicken growth performance by regulating fat metabolism.
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http://dx.doi.org/10.1111/1751-7915.13841DOI Listing
July 2021

Knowledge, attitude, and practice of medication among Haikou residents.

Ann Palliat Med 2021 Jun;10(6):6883-6891

Hainan General Hospital/Hainan Affiliated Hospital of Hainan Medical University, Haikou, China.

Background: Our study aims to explore the knowledge, attitude, and practice (KAP) and its influencing factors of medication among residents in Haikou, the capital city of Hainan Province, and inform the development of interventions to reduce residents' medication errors.

Methods: A cross-sectional questionnaire survey was conducted to investigate the KAP of medication among Haikou residents and its influencing factors from March to September 2019.

Results: A total of 471 valid questionnaires were collected (245 online and 226 offline), with an effective recovery rate of 94.2%. The average score of KAP of medication were 52.2±13.08, 27.34±8.14, and 51.54±9.22, respectively. The knowledge score reached "good" in the evaluation criteria of the questionnaire, and the attitude and practice scores were "fair". Multiple linear regression analysis revealed the medication knowledge increased with age; a lower education degree was associated with less knowledge and more medication errors, and a higher education level was associated with more access to medication knowledge.

Conclusions: Education on rational drug use should be performed via multiple ways to promote rational drug use and reduce risky medication behaviors, particularly among residents with low education degrees, e.g., drug counseling and guidance, regularly push medication science popularization, public welfare lecture on rational drug use, organize and compile popular science books.
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http://dx.doi.org/10.21037/apm-21-1295DOI Listing
June 2021

An Integrated Model-Based and Data-Driven Gap Metric Method for Fault Detection and Isolation.

IEEE Trans Cybern 2021 Jul 8;PP. Epub 2021 Jul 8.

This article proposes an integrated approach of model-based and data-driven gap metric fault detection and isolation in a stochastic framework. For actuator and sensor faults, an adaptive Kalman filter combining with the generalized likelihood ratio method is suggested. For component faults, especially incipient faults, the model-based scheme maybe not a good choice due to the existence of disturbances or noises. Hence, a novel data-driven gap metric strategy is presented. The design of the appropriate fault cluster center model and radius via the gap metric technique is put forward to enhance the isolability of the incipient faults. Numerical simulation results are given to demonstrate the effectiveness of the proposed fault detection and isolation algorithm.
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http://dx.doi.org/10.1109/TCYB.2021.3086193DOI Listing
July 2021

A commentary on "Radiofrequency ablation reduces pain for knee osteoarthritis: A meta-analysis of randomized controlled trials" (Int J Surg 2021:105951).

Int J Surg 2021 07 29;91:106008. Epub 2021 Jun 29.

Department of Medical Imaging, The 80th Army Hospital of PLA, Shandong Province, 261021, China. Electronic address:

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http://dx.doi.org/10.1016/j.ijsu.2021.106008DOI Listing
July 2021

Mixtures of rare earth elements show antagonistic interactions in Chlamydomonas reinhardtii.

Environ Pollut 2021 Oct 14;287:117594. Epub 2021 Jun 14.

Biophysical Environmental Chemistry Group, University of Montreal, P.O. Box 6128, Succ. Centre-Ville, Montreal, QC, Canada. Electronic address:

In order to better understand the environmental risks of the rare earth elements (REEs), it is necessary to determine their fate and biological effects under environmentally relevant conditions (e.g. at low concentrations, REE mixtures). Here, the unicellular freshwater microalga, Chlamydomonas reinhardtii, was exposed for 2 h to one of three soluble REEs (Ce, Tm, Y) salts at 0.5 μM or to an equimolar mixture of these REEs. RNA sequencing revealed common biological effects among the REEs. Known functions of the differentially expressed genes support effects of REEs on protein processing in the endoplasmic reticulum, phosphate transport and the homeostasis of Fe and Ca. The only stress response detected was related to protein misfolding in the endoplasmic reticulum. When the REEs were applied as a mixture, antagonistic effects were overwhelmingly observed with transcriptomic results suggesting that the REEs were initially competing with each other for bio-uptake. Metal biouptake results were consistent with this interpretation. These results suggest that the approach of government agencies to regulate the REEs using biological effects data from single metal exposures may be a largely conservative approach.
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http://dx.doi.org/10.1016/j.envpol.2021.117594DOI Listing
October 2021

Effects of temperature on the growth and carbohydrate production of three benthic dinoflagellate species from Hainan Island, South China Sea.

Ecotoxicology 2021 Jun 17. Epub 2021 Jun 17.

College of Life Science and Technology, Jinan University, Guangzhou, China.

Dinoflagellates in the genera Ostreopsis and Gambierdiscus are typical members of the marine benthic community particularly in tropical areas. Their geographic distribution has shown an increasing expansion towards temperate waters recently due to the global warming and climate changes; however, the knowledge is little of how the increasing temperatures might influence the physiological characteristics of Ostreopsis spp. and Gambierdiscus spp. Here, we carried out experiments to understand the effect of different temperatures on the growth, chlorophyll a content, and carbohydrate yield of Chinese strains of Ostreopsis cf. ovata, O. lenticularis, and Gambierdiscus caribaeus. Specifically, seven temperatures (15, 17.5, 20, 25, 30, 32.5, and 35 °C) were set for the two Ostreopsis species and five temperatures (15, 20, 25, 30, and 35 °C) were set for G. caribaeus. Our results suggested that both Ostreopsis (both species were 17.5-32.5 °C) and Gambierdiscus (20-35 °C) could survive a wide range of temperatures, consistent with the record worldwide. Cell density and chlorophyll a content were observed to be higher at high temperatures (30 and 32.5 °C) for both Ostreopsis species whereas G. caribaeus reached the maximum cell density and highest growth rate at 20 °C. Higher carbohydrate yield was detected in the suboptimal temperatures for all three dinoflagellates especially during the decaying phase. Our study reveals the optimal temperatures for the growth of three benthic harmful dinoflagellate species and provides insight into how the increasing temperature will affect their abundance as well as distribution.
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http://dx.doi.org/10.1007/s10646-021-02428-zDOI Listing
June 2021

Transcriptomic Landscape of Circulating Mononuclear Phagocytes in Langerhans Cell Histiocytosis at Single-cell Level.

Blood 2021 Jun 16. Epub 2021 Jun 16.

Affiliated Hospital of Academy of Military Medical Sciences, Beijing, China.

Langerhans cell histiocytosis (LCH) is an inflammatory myeloid neoplasm caused by aberrant activation of the mitogen-activated protein kinase (MAPK) pathway. Circulating myeloid cells from patients often carry disease-associated mutations and can be differentiated into langerinhigh LCH-like cells in vitro, but their detailed immune-phenotypic and molecular profiles are lacking and could shed key insights into disease biology. Here we recruited 217 pediatric LCH patients and took blood and tissue samples for BRAFV600E analysis. Immune-phenotyping of the circulating Lin-HLA-DR+ immune population in 49 of these patients revealed that decreased frequency of pDC was significantly linked to disease severity. By single-cell RNA sequencing of samples from 14 patients, we identified key changes in expression of RAS-MAPK-ERK signaling-related genes and transcription factors in distinct members of the mononuclear phagocyte system in the presence of BRAFV600E. Moreover, treatment of patients with the BRAF inhibitor Dabrafenib resulted in MAPK cascade inhibition, inflammation prevention, and regulation of cellular metabolism within mononuclear phagocytes. Finally, we also observed elevated expression of RAS-MAPK-ERK signaling-related genes in a CD207+CD1a+ cell subcluster in skin. Taken together, our data extends the molecular understanding of LCH biology at single-cell resolution, which might contribute to improvement of clinical diagnostics and therapeutics, and aid in the development of personalized medicine approaches.
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http://dx.doi.org/10.1182/blood.2020009064DOI Listing
June 2021

Automated tumor proportion scoring for PD-L1 expression based on multistage ensemble strategy in non-small cell lung cancer.

J Transl Med 2021 06 7;19(1):249. Epub 2021 Jun 7.

Department of Pathology, Molecular Pathology Research Center, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100730, China.

Introduction: Programmed cell death ligand-1 (PD-L1) expression is a promising biomarker for identifying treatment related to non-small cell lung cancer (NSCLC). Automated image analysis served as an aided PD-L1 scoring tool for pathologists to reduce inter- and intrareader variability. We developed a novel automated tumor proportion scoring (TPS) algorithm, and evaluated the concordance of this image analysis algorithm with pathologist scores.

Methods: We included 230 NSCLC samples prepared and stained using the PD-L1(SP263) and PD-L1(22C3) antibodies separately. The scoring algorithm was based on regional segmentation and cellular detection. We used 30 PD-L1(SP263) slides for algorithm training and validation.

Results: Overall, 192 SP263 samples and 117 22C3 samples were amenable to image analysis scoring. Automated image analysis and pathologist scores were highly concordant [intraclass correlation coefficient (ICC) = 0.873 and 0.737]. Concordances at moderate and high cutoff values were better than at low cutoff values significantly. For SP263 and 22C3, the concordances in squamous cell carcinomas were better than adenocarcinomas (SP263 ICC = 0.884 vs 0.783; 22C3 ICC = 0.782 vs 0.500). In addition, our automated immune cell proportion scoring (IPS) scores achieved high positive correlation with the pathologists TPS scores.

Conclusions: The novel automated image analysis scoring algorithm permitted quantitative comparison with existing PD-L1 diagnostic assays and demonstrated effectiveness by combining cellular and regional information for image algorithm training. Meanwhile, the fact that concordances vary in different subtypes of NSCLC samples, which should be considered in algorithm development.
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http://dx.doi.org/10.1186/s12967-021-02898-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8185941PMC
June 2021

Attention Enhancement for Exoskeleton-Assisted Hand Rehabilitation Using Fingertip Haptic Stimulation.

Front Robot AI 2021 21;8:602091. Epub 2021 May 21.

Department of Mechanical Engineering, University College London, London, United Kingdom.

Active enrollment in rehabilitation training yields better treatment outcomes. This paper introduces an exoskeleton-assisted hand rehabilitation system. It is the first attempt to combine fingertip cutaneous haptic stimulation with exoskeleton-assisted hand rehabilitation for training participation enhancement. For the first time, soft material 3D printing techniques are adopted to make soft pneumatic fingertip haptic feedback actuators to achieve cheaper and faster iterations of prototype designs with consistent quality. The fingertip haptic stimulation is synchronized with the motion of our hand exoskeleton. The contact force of the fingertips resulted from a virtual interaction with a glass of water was based on data collected from normal hand motions to grasp a glass of water. System characterization experiments were conducted and exoskeleton-assisted hand motion with and without the fingertip cutaneous haptic stimulation were compared in an experiment involving healthy human subjects. Users' attention levels were monitored in the motion control process using a Brainlink EEG-recording device and software. The results of characterization experiments show that our created haptic actuators are lightweight (6.8 ± 0.23 g each with a PLA fixture and Velcro) and their performance is consistent and stable with small hysteresis. The user study experimental results show that participants had significantly higher attention levels with additional haptic stimulations compared to when only the exoskeleton was deployed; heavier stimulated grasping weight (a 300 g glass) was associated with significantly higher attention levels of the participants compared to when lighter stimulated grasping weight (a 150 g glass) was applied. We conclude that haptic stimulations increase the involvement level of human subjects during exoskeleton-assisted hand exercises. Potentially, the proposed exoskeleton-assisted hand rehabilitation with fingertip stimulation may better attract user's attention during treatment.
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http://dx.doi.org/10.3389/frobt.2021.602091DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8176106PMC
May 2021

Phytoplankton community organization and succession by sea warming: A case study in thermal discharge area of the northern coastal seawater of China.

Mar Pollut Bull 2021 Aug 30;169:112538. Epub 2021 May 30.

Research Center of Harmful Algae and Marine Biology, Jinan University, Guangzhou 510632, PR China; Southern Marine Science and Engineering Guangdong Laboratory, Zhuhai, PR China. Electronic address:

Thermal discharges from power plants elevate temperatures in the surrounding waters, and this warming of the marine environment inevitably influences phytoplankton communities. In this study, we conducted seasonal sampling in coastal waters of the Bohai Sea off Qinhuangdao, China. The results revealed that the coastal waters are notably affected by thermal discharge, with unseasonably-high water temperatures, particularly during winter. The change of phytoplankton community structure has been detected in the warmer coastal seawater. Although diatoms were the dominant species during field investigations, there were no significant seasonal changes in diatom abundance. In contrast, the density of dinoflagellates was low in all seasons due to the effects of thermal discharge. Consequently, the general trend in phytoplankton succession was interrupted. In general, the findings of this study may provide further insights into the warmer environmental impacts on phytoplankton communities in temperate regions.
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http://dx.doi.org/10.1016/j.marpolbul.2021.112538DOI Listing
August 2021

Path planning for the Platonic solids on prescribed grids by edge-rolling.

PLoS One 2021 2;16(6):e0252613. Epub 2021 Jun 2.

School of Civil and Mechanical Engineering, Curtin University, Bentley, WA, Australia.

The five Platonic solids-tetrahedron, cube, octahedron, dodecahedron, and icosahedron-have found many applications in mathematics, science, and art. Path planning for the Platonic solids had been suggested, but not validated, except for solving the rolling-cube puzzles for a cubic dice. We developed a path-planning algorithm based on the breadth-first-search algorithm that generates a shortest path for each Platonic solid to reach a desired pose, including position and orientation, from an initial one on prescribed grids by edge-rolling. While it is straightforward to generate triangular and square grids, various methods exist for regular-pentagon tiling. We chose the Penrose tiling because it has five-fold symmetry. We discovered that a tetrahedron could achieve only one orientation for a particular position.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0252613PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8171926PMC
June 2021

Modulation of lactate-lysosome axis in dendritic cells by clotrimazole potentiates antitumor immunity.

J Immunother Cancer 2021 05;9(5)

State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, China

Background: Dendritic cells (DCs) play a critical role in antitumor immunity, but the therapeutic efficacy of DC-mediated cancer vaccine remains low, partly due to unsustainable DC function in tumor antigen presentation. Thus, identifying drugs that could enhance DC-based antitumor immunity and uncovering the underlying mechanism may provide new therapeutic options for cancer immunotherapy.

Methods: In vitro antigen presentation assay was used for DC-modulating drug screening. The function of DC and T cells was measured by flow cytometry, ELISA, or qPCR. B16, MC38, CT26 tumor models and C57BL/6, Balb/c, nude, and mice were used to analyze the in vivo therapy efficacy and impact on tumor immune microenvironment by clotrimazole treatment.

Results: By screening a group of small molecule inhibitors and the US Food and Drug Administration (FDA)-approved drugs, we identified that clotrimazole, an antifungal drug, could promote DC-mediated antigen presentation and enhance T cell response. Mechanistically, clotrimazole acted on hexokinase 2 to regulate lactate metabolic production and enhanced the lysosome pathway and expression in DCs subsequently induced DC maturation and T cell activation. Importantly, in vivo clotrimazole administration induced intratumor immune infiltration and inhibited tumor growth depending on both DCs and CD8+ T cells and potentiated the antitumor efficacy of anti-PD1 antibody.

Conclusions: Our findings showed that clotrimazole could trigger DC activation via the lactate-lysosome axis to promote antigen cross-presentation and could be used as a potential combination therapy approach to improving the therapeutic efficacy of anti-PD1 immunotherapy.
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http://dx.doi.org/10.1136/jitc-2020-002155DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8141455PMC
May 2021

Comparison of Prior Bridging Intravenous Thrombolysis With Direct Endovascular Thrombectomy for Anterior Circulation Large Vessel Occlusion: Systematic Review and Meta-Analysis.

Front Neurol 2021 30;12:602370. Epub 2021 Apr 30.

The Third ward of Neurology Department, Affiliated Hospital of Hebei University, Baoding, China.

Whether bridging treatment combining intravenous thrombolysis (IVT) and endovascular thrombectomy (EVT) is superior to direct EVT alone for emergent large vessel occlusion (LVO) in the anterior circulation is unknown. A systematic review and a meta-analysis were performed to investigate and assess the effect and safety of bridging treatment . direct EVT in patients with LVO in the anterior circulation. PubMed, EMBASE, and the Cochrane library were searched to assess the effect and safety of bridging treatment and direct EVT in LVO. Functional independence, mortality, asymptomatic and symptomatic intracranial hemorrhage (aICH and sICH, respectively), and successful recanalization were evaluated. The risk ratio and the 95% CI were analyzed. Among the eight studies included, there was no significant difference in the long-term functional independence (OR = 1.008, 95% CI = 0.845-1.204, = 0.926), mortality (OR = 1.060, 95% CI = 0.840-1.336, = 0.624), recanalization rate (OR = 1.015, 95% CI = 0.793-1.300, = 0.905), and the incidence of sICH (OR = 1.320, 95% CI = 0.931-1.870, = 0.119) between bridging therapy and direct EVT. After adjusting for confounding factors, bridging therapy showed a lower recanalization rate (effect size or ES = -0.377, 95% CI = -0.684 to -0.070, = 0.016), but there was no significant difference in the long-term functional independence (ES = 0.057, 95% CI = -0.177 to 0.291, = 0.634), mortality (ES = 0.693, 95% CI = -0.133 to 1.519, = 0.100), and incidence of sICH (ES = -0.051, 95% CI = -0.687 to 0.585, = 0.875) compared with direct EVT. Meanwhile, in the subgroup analysis of RCT, no significant difference was found in the long-term functional independence (OR = 0.927, 95% CI = 0.727-1.182, = 0.539), recanalization rate (OR = 1.331, 95% CI = 0.948-1.867, = 0.099), mortality (OR = 1.072, 95% CI = 0.776-1.481, = 0.673), and sICH incidence (OR = 1.383, 95% CI = 0.806-2.374, = 0.977) between patients receiving bridging therapy and those receiving direct DVT. For stroke patients with acute anterior circulation occlusion and who are eligible for intravenous thrombolysis, there is no significant difference in the clinical effect between direct EVT and bridging therapy, which needs to be verified by more randomized controlled trials.
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http://dx.doi.org/10.3389/fneur.2021.602370DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8120007PMC
April 2021

Diastereoselective Synthesis of Tetracyclic Tetrahydroquinoline Derivative Enabled by Multicomponent Reaction of Isocyanide, Allenoate, and 2-Aminochalcone.

Org Lett 2021 Jun 13;23(11):4094-4098. Epub 2021 May 13.

Department of Chemistry, College of Sciences & Institute for Sustainable Energy, Shanghai University, 99 Shangda Road, Shanghai 200444, People's Republic of China.

We report here a multicomponent protocol to assemble several polycyclic dihydropyran-fused tetrahydroquinoline structures with excellent diastereoselectivity. This procedure employs simple feedstocks to accomplish a series of diverse structures, which is difficult to attain by traditional sequences.
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http://dx.doi.org/10.1021/acs.orglett.1c00912DOI Listing
June 2021

Repair of Defects in the Scalp and Face With Expanded Superficial Temporal Artery Flaps: A Single-Center Retrospective Study.

J Craniofac Surg 2021 Sep;32(6):2155-2158

Department of Plastic Surgery, Jinling Hospital, Bengbu Medical College, Bengbu, Anhui.

Background And Objective: Defects resulted from the removal of large scars, benign tumors, severe pigmentation abnormalities, and vascular malformations, etc., in the scalp and face need to be repaired to restore the appearance. Here, the authors introduced the application of various expanded superficial temporal artery (STA) flaps in the repair of above defects.

Methods: From Jan. 2015 to Dec. 2018, 19 patients with craniofacial secondary defects received the repair with expanded STA flaps in our clinic. The defects were resulted from the removal of scalp scar (n = 6), neurofibroma (n = 4), sebaceous nevus (n = 3), arteriovenous malformation (n = 2), facial scar (n = 2), and port-wine stain (n = 2). The expanded STA flaps included 14 cases of flaps pedicled by parietal branch of STA, 2 cases of flaps pedicled by parietal branch of STA combined with laser hair removal, 1 case of flaps pedicled by frontal branch of STA, and 2 cases of prefabricated expanded skin flap with the superficial temporal fascia in the neck.

Results: The two-stage operation and water-filling expansion were accomplished in all patients. All flaps survived well, except one flap with venous congestion, which resolved after blood-letting and application of drugs promoting venous draining. In the three to six months follow-up, the flaps' color, texture, and thickness were satisfying.

Conclusions: Individual application of different types of expanded STA flaps could achieve ideal results in repairing craniofacial secondary defects.
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http://dx.doi.org/10.1097/SCS.0000000000007531DOI Listing
September 2021

[Effect of urease/nitrification inhibitor on urea nitrogen conversion in black soil and cinnamon soil.]

Ying Yong Sheng Tai Xue Bao 2021 Apr;32(4):1352-1360

Jinxi Natural Gas Chemical Co. Ltd., Huludao 125001, Liaoning, China.

We studied the effects of urease/nitrification inhibitor combinations on urea hydrolysis and nitrification, aiming to screen out the effective inhibitor combinations for black soil and cinnamon soil in Northeast China. Urease inhibitor, N-butyl thiophosphate-triamine (NBPT), and its combination with nitrification inhibitor dicyandiamide (DCD), 3, 4-dimethylpyrazole phosphate (DMPP), 2-chloro-6 (trichloromethyl)-pyridine (CP), 2-amino-4-chloro-6-methylpyrimidine (AM) and 3-methylpyrazole (MP) were added to urea separately. Samples were collected 15 times in each of all the treatments during 125 days. We examined the changes of urea nitrogen, ammo-nium, nitrate, and nitrification inhibition rate in the two soils. Our results showed the hydrolysis of urea in black soil and cinnamon soil was about 7 d, and the addition of NBPT with or without diffe-rent nitrification inhibitors slowed down the hydrolysis to 21 d at least. Compared with the treatment with common urea, inhibitor addition significantly increased soil NH-N, decreased soil NO-N, and maintained the high NH-N content in soil for a longer time. In black soil, application with nitrification inhibitor inhibited soil nitrification significantly and lasted for more than 125 d. DMPP and CP combined with NBPT increased the NH-N content in black soil by 1.6-1.8 times, while the nitrification inhibition rate was 47.9% and 24.1% at 125 d, respectively. In the cinnamon soil, the application of nitrification inhibitor could prolong the duration of ammonium oxidation from 80 d to 110 d. DCD and DMPP combined with NBPT increased the NH-N content in cinnamon soil by 2.1-3.4 times, while the nitrification inhibition rates at 125 d were 25.3% and 23.2%, respectively. Therefore, NBPT+DMPP combination with urea was recommended for utilization in black soil, followed by NBPT+CP. In cinnamon soil, NBPT+DCD combination with urea was recommended, followed by NBPT+DMPP.
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http://dx.doi.org/10.13287/j.1001-9332.202104.023DOI Listing
April 2021

Artemisinin derivatives inhibit adipogenic differentiation of 3T3-L1 preadipocytes through upregulation of CHOP.

Biochem Biophys Res Commun 2021 06 21;557:309-315. Epub 2021 Apr 21.

Department of Central Laboratory, Beijing Shijitan Hospital, Capital Medical University, Beijing, 100038, China; Department of Plastic and Cosmetic Surgery, Beijing Shijitan Hospital, Capital Medical University, Beijing, 100038, China. Electronic address:

Artemisinin derivatives could inhibit adipogenic differentiation of 3T3-L1 preadipocytes and prevent obesity in mice. However, the molecular mechanism remains largely unclear. Our research was designed to investigate the specific molecular target of artemisinin derivatives in adipogenic differentiation of 3T3-L1 preadipocytes. Here, we revealed that in response to dihydroartemisinin (DHA) or artesunate (ATS), intracellular lipid was decreased in a concentration dependent manner as shown by BODIPY staining. Quantitative PCR analysis showed that expression of Cebpa, Pparg, Fabp4 and Plin was significantly decreased by DHA treatment in a concentration and time dependent manner. Also, DHA treatment remarkably downregulated expression of CCAAT/enhancer-binding protein α (C/EBPα) and nuclear receptor peroxisome proliferation-activated receptor γ (PPARγ) of adipogenic induced 3T3-L1 cells as assayed by western blotting. RNA-seq analysis identified thousands of differential expression genes (DEGs), among which CHOP expression was significantly improved in DHA treated cells. Upregulation of CHOP was verified by quantitative PCR and western blotting, respectively. Knockdown of CHOP by the specific shRNA revealed that the inhibition of adipogenesis by DHA was strongly blocked, resulting in restored lipid accumulation and expression of adipogenic molecules. In conclusions, the inhibitory effect of DHA on adipogenic differentiation of 3T3-L1 preadipocytes was exerted in a concentration and time dependent manner, which was mediated by expression of CHOP.
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http://dx.doi.org/10.1016/j.bbrc.2021.04.013DOI Listing
June 2021
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