Publications by authors named "Le Yang"

436 Publications

Magnetic Resonance Imaging Investigation of Neuroplasticity After Ischemic Stroke in Tetramethylpyrazine-Treated Rats.

Front Pharmacol 2022 26;13:851746. Epub 2022 Apr 26.

School of Traditional Chinese Medicine, Capital Medical University, Beijing, China.

Ischemic stroke elicits white matter injury typically signed by axonal disintegration and demyelination; thus, the development of white matter reorganization is needed. 2,3,5,6-Tetramethylpyrazine (TMP) is widely used to treat ischemic stroke. This study was aimed to investigate whether TMP could protect the white matter and promote axonal repair after cerebral ischemia. Male Sprague-Dawley rats were subjected to permanent middle cerebral artery occlusion (MCAO) and treated with TMP (10, 20, 40 mg/kg) intraperitoneally for 14 days. The motor function related to gait was evaluated by the gait analysis system. Multiparametric magnetic resonance imaging (MRI) was conducted to noninvasively identify gray-white matter structural integrity, axonal reorganization, and cerebral blood flow (CBF), followed by histological analysis. The expressions of axonal growth-associated protein 43 (GAP-43), synaptophysin (SYN), axonal growth-inhibitory signals, and guidance factors were measured by Western blot. Our results showed TMP reduced infarct volume, relieved gray-white matter damage, promoted axonal remodeling, and restored CBF along the peri-infarct cortex, external capsule, and internal capsule. These MRI findings were confirmed by histopathological data. Moreover, motor function, especially gait impairment, was improved by TMP treatment. Notably, TMP upregulated GAP-43 and SYN and enhanced axonal guidance cues such as Netrin-1/DCC and Slit-2/Robo-1 but downregulated intrinsic growth-inhibitory signals NogoA/NgR/RhoA/ROCK-2. Taken together, our data indicated that TMP facilitated poststroke axonal remodeling and motor functional recovery. Moreover, our findings suggested that TMP restored local CBF, augmented guidance cues, and restrained intrinsic growth-inhibitory signals, all of which might improve the intracerebral microenvironment of ischemic areas and then benefit white matter remodeling.
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http://dx.doi.org/10.3389/fphar.2022.851746DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9086494PMC
April 2022

Author Correction: Floating solid-state thin films with dynamic structural colour.

Nat Nanotechnol 2022 May;17(5):560

Department of Engineering Product Design, Singapore University of Technology and Design, Singapore, Singapore.

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http://dx.doi.org/10.1038/s41565-022-01128-xDOI Listing
May 2022

Regulatory Mechanism of Trap Formation in the Nematode-Trapping Fungi.

J Fungi (Basel) 2022 Apr 16;8(4). Epub 2022 Apr 16.

State Key Laboratory for Conservation and Utilization of Bio-Resources, Key Laboratory for Microbial Resources of the Ministry of Education, School of Life Sciences, Yunnan University, Kunming 650091, China.

Nematode-trapping (NT) fungi play a significant role in the biological control of plant- parasitic nematodes. NT fungi, as a predator, can differentiate into specialized structures called "traps" to capture, kill, and consume nematodes at a nutrient-deprived condition. Therefore, trap formation is also an important indicator that NT fungi transition from a saprophytic to a predacious lifestyle. With the development of gene knockout and multiple omics such as genomics, transcriptomics, and metabolomics, increasing studies have tried to investigate the regulation mechanism of trap formation in NT fungi. This review summarizes the potential regulatory mechanism of trap formation in NT fungi based on the latest findings in this field. Signaling pathways have been confirmed to play an especially vital role in trap formation based on phenotypes of various mutants and multi-omics analysis, and the involvement of small molecule compounds, woronin body, peroxisome, autophagy, and pH-sensing receptors in the formation of traps are also discussed. In addition, we also highlight the research focus for elucidating the mechanism underlying trap formation of NT fungi in the future.
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http://dx.doi.org/10.3390/jof8040406DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9031305PMC
April 2022

Effects of mokF gene deletion and overexpression on the Monacolin K metabolism yields of Monascus purpureus.

Appl Microbiol Biotechnol 2022 Apr 18;106(8):3069-3080. Epub 2022 Apr 18.

Beijing Technology & Business University (BTBU), Beijing, 100048, China.

Monascus purpureus is a fungus known for producing various physiologically active secondary metabolites. Of these, Monacolin K, a compound with hypocholesterolemic effects, is controlled by the biosynthetic gene mokF. Here, mokF deletion and overexpression strains (F2 and C3, respectively) were constructed using genetic engineering and compared with the M. purpureus wild strain (M1). The results showed that Monacolin K production was reduced by 50.86% in F2 and increased by 74.19% in C3. Of the three strains, C3 showed the highest production of Monacolin K and the most abnormal morphology. In addition, mokF influenced the expression level of mokA-mokI and might play an important role in regulating the biosynthesis of secondary metabolites in M. purpureus. Overall, our study verified the function of mokF in M. purpureus using gene deletion and overexpression technology. KEY POINTS: • The deletion and overexpression strains of mokF gene were successfully constructed. • The deletion or overexpression of mokF gene directly affected Monacolin K production. •The mokF gene had little effect on Monascus pigments and cell biomass.
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http://dx.doi.org/10.1007/s00253-022-11913-2DOI Listing
April 2022

A Brief Review of circRNA Biogenesis, Detection, and Function.

Curr Genomics 2021 Dec;22(7):485-495

Department of Basic teaching, Jiangxi Business School, Nanchang, China.

Circular RNA (circRNA) is a non-coding molecule produced through alternative splicing of one or more exons of a gene in the presence of an RNA-induced silencing complex (RISC). Its formation depends on complementary intron sequences on both sides of the circularized sequence. CircRNA functions as a sponge for miRNA, playing the role of the transcriptional regulator or potential biomarker. It has an impact on fetal growth and on synaptic facilitation in the brain. In this review, we illustrate biogenesis mechanisms, characteristics, and functions of cirRNAs. We also summarize methods using sequence feature and RNA next-generation sequencing data for circRNA prediction. Finally, we discuss the state of the research on circRNA in diseases, which will bring new contributions to future disease treatments.
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http://dx.doi.org/10.2174/1389202922666210331130722DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8905635PMC
December 2021

Simultaneous determination of almonertinib and its active metabolite HAS-719 in human plasma by LC-MS/MS: Evaluation of pharmacokinetic interactions.

J Chromatogr B Analyt Technol Biomed Life Sci 2022 May 24;1197:123231. Epub 2022 Mar 24.

Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 501 Haike Road, Shanghai 201203, China; University of Chinese Academy of Sciences, No.19A Yuquan Road, Beijing 100049, China. Electronic address:

The combination of two or more drugs in a clinical setting has an impact in pharmacokinetics, drug efficacy and safety, and the study of these interactions has attracted considerable attention over the last years. In the present study, we have developed a LC-MS/MS method for the sensitive and reliable quantification of almonertinib and its active metabolite HAS-719. Further, we investigated the effects of their pharmacokinetics in humans by using modulators of CYP3A, an almonertinib-metabolizing enzyme. Analytes were extracted from plasma samples via acetonitrile-induced protein precipitation and separated on a BEH C18 column using ammonium acetate with formic acid and acetonitrile as the mobile phase. Electrospray ionization in positive ion mode and multiple reaction monitoring were used to monitor the ion transitions at m/z 526 → 411 and 512 → 423. Validation was performed in the range 0.500 to 500 ng/mL for both the analytes of interest according to the guidelines of the U.S. Food and Drug Administration and European Medicines Agency, sufficient to account for variations in plasma concentrations caused by the presence of CYP3A modulators. The selectivity, precision, accuracy, recovery and matrix effect of this method were all within acceptable limits of bioanalytics. The interference of CYP3A modulators itraconazole and rifampicin with the analytes, and the mutual interference between the analytes were also investigated producing acceptable results. The method herein described was successfully applied for the pharmacokinetics evaluation of almonertinib in healthy subjects exposed to a single dose of almonertinib (110 mg), with or without itraconazole or rifampicin.
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http://dx.doi.org/10.1016/j.jchromb.2022.123231DOI Listing
May 2022

Photon-upconverters for blue organic light-emitting diodes: a low-cost, sky-blue example.

Nanoscale Adv 2022 Mar 24;4(5):1318-1323. Epub 2022 Jan 24.

State Key Laboratory of Modern Optical Instrumentation, College of Optical Science and Engineering, International Research Center for Advanced Photonics, Zhejiang University Hangzhou 310027 China.

In the research ecosystem's quest towards having deployable organic light-emitting diodes with higher-energy emission (, blue light), we advocate focusing on fluorescent emitters, due to their relative stability and colour purity, and developing design strategies to significantly improve their efficiencies. We propose that all triplet-triplet annihilation upconversion (TTA-UC) emitters would make good candidates for triplet fusion-enhanced OLEDs ("FuLEDs"), due to the energetically uphill nature of the photophysical process, and their common requirements. We demonstrate this with the low-cost sky-blue 1,3-diphenylisobenzofuran (DPBF). Having satisfied the criteria for TTA-UC, we show DPBF as a photon upconverter ( 92 mW cm), and henceforth demonstrate it as a bright emitter for FuLEDs. Notably, the devices achieved 6.5% external quantum efficiency (above the ∼5% threshold without triplet contribution), and triplet-exciton-fusion-generated fluorescence contributes up to 44% of the electroluminescence, as shown by transient measurements. Here, triplet fusion translates to a quantum yield ( ) of 19%, at an electrical excitation of ∼0.01 mW cm. The enhancement is meaningful for commercial blue OLED displays. We also found DPBF to have decent hole mobilities of ∼0.08 cm V s. This additional finding can lead to DPBF being used in other capacities in various printable electronics.
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http://dx.doi.org/10.1039/d1na00803jDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8886671PMC
March 2022

The Impact of Ischemic Stroke on Gray and White Matter Injury Correlated With Motor and Cognitive Impairments in Permanent MCAO Rats: A Multimodal MRI-Based Study.

Front Neurol 2022 2;13:834329. Epub 2022 Mar 2.

School of Traditional Chinese Medicine, Capital Medical University, Beijing, China.

Background: Identifying the alterations of the cerebral gray and white matter is an important prerequisite for developing potential pharmacological therapy for stroke. This study aimed to assess the changes of gray and white matter after permanent middle cerebral artery occlusion (pMCAO) in rats using magnetic resonance imaging (MRI), and to correlate them with the behavior performance.

Methods: Rats were subjected to pMCAO or sham surgery and reared for 30 days. Motor and cognitive function of the rats were examined by gait and Morris water maze (MWM) tests, respectively. Multimodal MRI was conducted to examine the functional and structural changes of the gray and white matter followed with luxol fast blue (LFB) staining.

Results: The gait and MWM tests revealed significant motor and cognitive dysfunction in pMCAO rats, respectively. Magnetic resonance angiography presented abnormal intracranial arteries in pMCAO rats with reduced signal intensity of the anterior cerebral artery, anterior communicating cerebral artery, internal carotid artery, and increased basilar artery vessel signal compared with sham rats. Arterial spin labeling confirmed the decreased cerebral blood flow in the infarcted sensorimotor cortex and striatum. Structural T2-weighted imaging and T2 mapping showed brain atrophy and elevation of T2 value in the gray (sensorimotor cortex, striatum) and white (external capsule, internal capsule) matter of pMCAO rats. The results from diffusion tensor imaging (DTI) corresponded well with LFB staining showing reduced relative FA accompanied with increased relative AD and RD in the gray and white matter of pMCAO rats compared with sham rats. Fiber tracking derived from DTI further observed significantly reduced fiber density and length in the corresponding brain regions of pMCAO rats compared with sham rats. Specially, the DTI parameters (especially FA) in the relevant gray matter and white matter significantly correlated with the behavior performance in the gait and MWM tests.

Conclusion: Collectively, the gray and white matter damages could be non-invasively monitored in pMCAO rats by multimodal MRI. DTI-derived parameters, particularly the FA, might be a good imaging index to stage gray and white matter damages associated with post-stroke motor and cognitive impairments.
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http://dx.doi.org/10.3389/fneur.2022.834329DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8924049PMC
March 2022

Phytoremediation of heavy metal pollution: Hotspots and future prospects.

Ecotoxicol Environ Saf 2022 Mar 11;234:113403. Epub 2022 Mar 11.

Department of Chemistry, Faculty of Science, University of Hradec Kralove, Hradec Kralove 500 03, Czech Republic. Electronic address:

To clarify the global status and research hotspots of heavy metal pollution phytoremediation, we used Web of Science, Cite Space software, and VOS viewer to analyse 1123 publications from the period of 2000-2020. Literature categories, research hotpots, and the most prolific publications by country, institution, and author were analysed separately. Around 34% of the articles are contributed from five countries: China (29.37%), India (11.00%), Spain (6.29%), Italy (6.20%), and Pakistan (5.67%). The hot research topic keywords were "diversity", "translocation", and "enhanced phytoremediation". Cadmium was the most highly concerned heavy metal in the phytoremediation. Twenty-three articles were highly cited, and they mainly focused on 1) enhancing the remediation ability of plants in heavy metal contaminated soil by microbial and chemical additives; 2) the molecular effect and mechanism of heavy metals on plant growth and development; 3) discovering novel heavy metal hyper-enriched plants which can remediate mixed heavy metal pollution. From the above analysis, we concluded that the future research directions should be 1) strengthening the plant remediation ability by biochemical means; 2) studying the molecular mechanism underlying heavy metal damage to plants; 3) studying the enrichment principle of plants for heavy metals. The present study provides a further understanding of the trends in phytoremediation of heavy metal pollution, and the data analysed can be used as a guide for future research directions.
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http://dx.doi.org/10.1016/j.ecoenv.2022.113403DOI Listing
March 2022

Modification of STIM2 by mA RNA methylation inhibits metastasis of cholangiocarcinoma.

Ann Transl Med 2022 Jan;10(2):40

Department of General Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Background: N6-methyladenosine (mA) is the most frequent internal methylation of eukaryotic RNA (ribonucleic acid) transcripts and plays an important function in RNA processing. The current research aimed to investigate the role of mA-STIM2 axis in cholangiocarcinoma (CCA) progression.

Methods: The expression of STIM2 (Stromal Interaction Molecule 2) in CCA was measured using quantitative polymerase chain reaction (PCR) and immunohistochemistry (IHC). STIM2 was examined for its effects on the malignant phenotypes of CCA cells. The mA modification of STIM2 was assessed through MeRIP (methylated RNA Immunoprecipitation)-PCR.

Results: Based on the GEPIA (Gene Expression Profiling Interactive Analysis) 2 database findings, a low STIM2 mRNA (messenger RNA) level was related to a poor prognosis in individuals with CCA. Quantitative PCR and IHC assays indicated decreased protein satin in CCA tissues and were associated with extrahepatic metastasis. Vianude mice tail vein injection model indicated that increased STIM2 levels suppressed CCA cell metastasis , while KRT8 (keratin 8) was detected as the direct downstream target of STIM2-mediated CCA cell metastasis . Meanwhile, based on SRAMP database and MeRIP assays indicated that mA alteration resulted in abnormal STIM2 expression in CCA via METTL14 and YTHDC2.

Conclusions: Our findings revealed the epi-transcriptomic dysregulation in CCA and metastasis by proposing a complicated STIM2-KRT8 regulatory paradigm based on mA alteration.
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http://dx.doi.org/10.21037/atm-21-6485DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8848379PMC
January 2022

Upcycling Silicon Photovoltaic Waste into Thermoelectrics.

Adv Mater 2022 May 28;34(19):e2110518. Epub 2022 Mar 28.

Agency for Science, Technology and Research, Institute of Materials Research and Engineering, #08-03, 2 Fusionopolis Way, Singapore, 138634, Singapore.

Two decades after the rapid expansion of photovoltaics, the number of solar panels reaching end-of-life is increasing. While precious metals such as silver and copper are usually recycled, silicon, which makes up the bulk of a solar cells, goes to landfills. This is due to the defect- and impurity-sensitive nature in most silicon-based technologies, rendering it uneconomical to purify waste silicon. Thermoelectrics represents a rare class of material in which defects and impurities can be engineered to enhance the performance. This is because of the majority-carrier nature, making it defect- and impurity-tolerant. Here, the upcycling of silicon from photovoltaic (PV) waste into thermoelectrics is enabled. This is done by doping 1% Ge and 4% P, which results in a figure of merit (zT) of 0.45 at 873 K, the highest among silicon-based thermoelectrics. The work represents an important piece of the puzzle in realizing a circular economy for photovoltaics and electronic waste.
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http://dx.doi.org/10.1002/adma.202110518DOI Listing
May 2022

Electrochemical Aerobic Oxidative Cleavage of (sp)C-C(sp)/H Bonds in Alkylarenes.

J Org Chem 2022 03 21;87(5):3286-3295. Epub 2022 Feb 21.

Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China.

An electrochemistry-promoted oxidative cleavage of (sp)C-C(sp)/H bonds in alkylarenes was developed. Various aryl alkanes can be smoothly converted into ketones/aldehydes under aerobic conditions using a user-friendly undivided cell setup. The features of air as oxidant, scalability, and mild conditions make them attractive in synthetic organic chemistry.
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http://dx.doi.org/10.1021/acs.joc.1c02947DOI Listing
March 2022

High-Performance Thermal Interface Materials with Magnetic Aligned Carbon Fibers.

Materials (Basel) 2022 Jan 19;15(3). Epub 2022 Jan 19.

Beijing Key Laboratory on Space Thermal Control Technology, Beijing Institute of Spacecraft System Engineering, Beijing 100094, China.

Thermal interface materials with high thermal conductivity and low hardness are crucial to the heat dissipation of high-power electronics. In this study, a high magnetic field was used to align the milled carbon fibers (CFs, 150 μm) in silicone rubber matrix to fabricate thermal interface materials with an ordered and discontinuous structure. The relationship among the magnetic field density, the alignment degree of CFs, and the properties of the resulting composites was explored by experimental study and theoretical analysis. The results showed higher alignment degree and enhanced thermal conductivity of composites under increased magnetic flux density within a certain curing time. When the magnetic flux density increased to 9 T, the CFs showed perfect alignment and the composite showed a high thermal conductivity of 11.76 W/(m·K) with only 20 vol% CF loading, owing to the ordered structure. Meanwhile, due to the low filler loading and discontinuous structure, a low hardness of 60~70 (shore 00) was also realized. Their thermal management performance was further confirmed in a test system, revealing promising applications for magnetic aligned CF-rubber composites in thermal interface materials.
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http://dx.doi.org/10.3390/ma15030735DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8836848PMC
January 2022

Decreased Expression of Programmed Death Ligand-L1 by Seven in Absentia Homolog 2 in Cholangiocarcinoma Enhances T-Cell-Mediated Antitumor Activity.

Front Immunol 2022 27;13:845193. Epub 2022 Jan 27.

Department of General Surgery, The Second Affiliated Hospital of Nanchang University, Nanchang, China.

N6-methyladenosine (m6A) has been reported as an important mechanism of post-transcriptional regulation. Programmed death ligand 1 (PD-L1) is a primary immune inhibitory molecule expressed on tumor cells that promotes immune evasion. In addition, seven in absentia homolog 2 (Siah2), a RING E3 ubiquitin ligase, has been involved in tumorigenesis and cancer progression. However, the role of m6A-METTL14-Siah2-PD-L1 axis in immunotherapy remains to be elucidated. In this study, we showed that METTL14, a component of the mA methyltransferase complex, induced Siah2 expression in cholangiocarcinoma (CCA). METTL14 was shown to enrich mA modifications in the 3'UTR region of the Siah2 mRNA, thereby promoting its degradation in an YTHDF2-dependent manner. Furthermore, co-immunoprecipitation experiments demonstrated that Siah2 interacted with PD-L1 by promoting its K63-linked ubiquitination. We also observed that and Siah2 knockdown inhibited T cells expansion and cytotoxicity by sustaining tumor cell PD-L1 expression. The METTL14-Siah2-PD-L1-regulating axis was further confirmed in human CCA specimens. Analysis of specimens from patients receiving anti-PD1 immunotherapy suggested that tumors with low Siah2 levels were more sensitive to anti-PD1 immunotherapy. Taken together, our results evidenced a new regulatory mechanism of Siah2 by METTL14-induced mRNA epigenetic modification and the potential role of Siah2 in cancer immunotherapy.
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http://dx.doi.org/10.3389/fimmu.2022.845193DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8828655PMC
March 2022

Contribution of recycled moisture to precipitation and its influencing factors in the subalpine zone of Qilian Mountains.

Environ Sci Pollut Res Int 2022 Feb 13. Epub 2022 Feb 13.

College of Geography and Environment Science, Northwest Normal University, Lanzhou, 730070, China.

Recycled moisture, the part of precipitating vapor that comes from surface evapotranspiration, plays an important role in the hydrological process and the feedback of the land surface-atmosphere system. In this study, we use the δH and δO data of precipitation, plant water, and surface water systematically collected in the subalpine area of the Qilian Mountains from May to October, 2019, and assess the contribution of recycled moisture to precipitation based on the three-component isotopic mixing model. The results indicated that the main source of precipitating vapor comes from the advected vapor, and its contribution reaches up to 87.8 ± 1.6%. The contribution of local recycled moisture is 12.2 ± 1.6%, in which the proportion of transpiration and evaporation are 8.9 ± 0.6% and 3.3 ± 1.0%, respectively. In terms of the correlation between recycled moisture and its influencing factors, the temperature has a positive effect on evapotranspiration and promotes moisture recycling, while precipitation and humidity have a certain inhibition effect on evapotranspiration and reduce the contribution of recycled moisture.
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http://dx.doi.org/10.1007/s11356-022-19107-zDOI Listing
February 2022

Association Between Autoimmune Diseases and Spontaneous Cervicocranial Arterial Dissection.

Front Immunol 2021 21;12:820039. Epub 2022 Jan 21.

Departments of Neurology, The First Affiliated Hospital of Soochow University, Suzhou, China.

A series of biopsies and reports showed autoimmune diseases might be involved in the process of local inflammation related to spontaneous cervicocranial arterial dissection (SCCAD) occurrence. This retrospective case-control study examined the association between SCCADs and autoimmune diseases in patients and control subjects from 2014 to 2020. SCCAD patients and age/sex-matched control subjects were recruited, and clinical data were collected. SCCAD was confirmed by digital subtraction angiography or high-resolution magnetic resonance imaging. The study included 215 SCCAD patients and 430 control subjects. Totally, 135 (62.8%) of the 215 cases were found SCCAD in the anterior circulation, 26 (12.0%) patients involved multiple vessels. Autoimmune disease occurred in 27 (12.6%) cases with SCCAD and 4 (0.9%) control subjects (p<0.001). A conditional multivariable logistic regression model was used to calculate the odds ratio for SCCAD among patients with a history of autoimmune disease, adjusting for hypertension, diabetes, hyperlipidemia, and smoking. After adjustment, autoimmune diseases were associated with SCCAD (p<0.001). After sub-analysis by a similar modeling strategy, significant associations were still observed in different subgroups, such as female group and male group as well as intramural hematoma (IMH) group and Non-IHM group. The association of SCCAD with autoimmune disease suggested that autoimmune mechanisms may be involved in some etiologies of SCCAD.
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http://dx.doi.org/10.3389/fimmu.2021.820039DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8814437PMC
March 2022

Effect of collagen casing on the quality characteristics of fermented sausage.

PLoS One 2022 3;17(2):e0263389. Epub 2022 Feb 3.

College of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China.

Objective: Fermented sausage is popular all over the world for its rich nutrition and unique flavor. Sausage casing is one of the key factors affecting the quality of fermented sausage. However, there is little information involved in this field.

Methods: In this study, collagen casings were used as a wrapping material, and natural casings (pig casings) were used as a control. The effects of the two types of casings on biogenic amine content and other quality characteristics of fermented sausage were analyzed with increasing the storage time.

Results: The results showed that with storage time increasing, the hardness and gumminess of fermented sausage in collagen casing (CC) group were higher than those in pig casing (PC) group (P<0.05), while the elasticity in CC group was lower than that in PC group (P<0.05). In the processing and storage period, there was no significant difference in the type and content of flavor substances between the two groups. More importantly, the contents of tryptamine, putrescine, cadaverine, histamine, tyramine and phenyethylamine in fermented sausage of CC group were lower than those in PC group (P<0.05).

Conclusion: In conclusion, this study revealed that CC could improve the quality characteristics of fermented sausage and reduce the content of biogenic amines in fermented sausage, which provides a theoretical basis for the choice of casings in industrial production in the future.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0263389PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8812867PMC
March 2022

A New Hierarchical Temporal Memory Algorithm Based on Activation Intensity.

Comput Intell Neurosci 2022 24;2022:6072316. Epub 2022 Jan 24.

Department of Computer Science and Telecommunication Engineer, Jiangsu University, Zhenjiang 212013, China.

As a human-cortex-inspired computing model, hierarchical temporal memory (HTM) has shown great promise in sequence learning and has been applied to various time-series applications. HTM uses the combination of columns and neurons to learn the temporal patterns within the sequence. However, the conventional HTM model compacts the input into two naive column states-active and nonactive, and uses a fixed learning strategy. This simplicity limits the representation capability of HTM and ignores the impacts of active columns on learning the temporal context. To address these issues, we propose a new HTM algorithm based on activation intensity. By introducing the column activation intensity, more useful and fine-grained information from the input is retained for sequence learning. Furthermore, a self-adaptive nonlinear learning strategy is proposed where the synaptic connections are dynamically adjusted according to the activation intensity of columns. Extensive experiments are carried out on two real-world time-series datasets. Compared to the conventional HTM and LSTM model, our method achieved higher accuracy and less time overhead.
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http://dx.doi.org/10.1155/2022/6072316DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8803450PMC
February 2022

LncRNA POT1-AS1 accelerates the progression of gastric cancer by serving as a competing endogenous RNA of microRNA-497-5p to increase PDK3 expression.

J Gastrointest Oncol 2021 Dec;12(6):2728-2742

Department of Gastroenterology, Shidong Hospital, Yangpu District, Shidong Hospital Affiliated to University of Shanghai for Science and Technology, Shanghai, China.

Background: Gastric cancer (GC) is the most common malignant tumor of the digestive system. Although progress has been reported in terms of treatment, it is still the second leading cause of cancer-related death. Long non-coding RNAs have been shown to play a key role in human cancers in recent investigations. However, the role of POT1-AS1 in GC is still unclear.

Methods: The relative POT1-AS1 level in GC tissues and paracancerous tissues was detected by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). The biological function of POT1-AS1 was studied by CCK8 and Transwell assays in vitro experiments. Moreover, the downstream target genes of POT1-AS1 were predicted by bioinformatics.

Results: In this study, high POT1-AS1 expression in GC cells was confirmed, and its elevated expression was linked to patients' negative clinicopathological characteristics, as well as shorter disease-free survival (DFS) and overall survival (OS). POT1-AS1 was shown to serve as a competing endogenous RNA (ceRNA) by sponging miR-497-5p to increase PDK3 expression. The impact of POT1-AS1 silencing on GC malignant phenotypes could be reversed by suppressing miR-497-5p or restoring PDK3, according to rescue experiments.

Conclusions: In brief, POT1-AS1 acted as an oncogenic lncRNA in GC, facilitating GC development by affecting the miR-497-5p/PDK3 axis, implying that the POT1-AS1/miR-497-5p/PDK3 axis is a useful target in anticancer therapy.
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http://dx.doi.org/10.21037/jgo-21-709DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8748042PMC
December 2021

Analyzing Quantitative Trait Loci for Fiber Quality and Yield-Related Traits From a Recombinant Inbred Line Population With Race as One Parent.

Front Plant Sci 2021 3;12:817748. Epub 2022 Jan 3.

College of Agronomy and Biotechnology, Southwest University, Chongqing, China.

Fiber quality and yield-related traits are important agronomic traits in cotton breeding. To detect the genetic basis of fiber quality and yield related traits, a recombinant inbred line (RIL) population consisting of 182 lines was established from a cross between cultivar CCRI35 and race accession TX-832. The RIL population was deeply genotyped using SLAF-seq and was phenotyped in six environments. A high-density genetic linkage map with 15,765 SNP markers and 153 SSR markers was constructed, with an average distance of 0.30 cM between adjacent markers. A total of 210 fiber quality quantitative trait loci (QTLs) and 73 yield-related QTLs were identified. Of the detected QTLs, 62 fiber quality QTLs and 10 yield-related QTLs were stable across multiple environments. Twelve and twenty QTL clusters were detected on the At and Dt subgenome, respectively. Twenty-three major QTL clusters were further validated through associated analysis and five candidate genes of four stable fiber quality QTLs were identified. This study revealed elite loci influencing fiber quality and yield and significant phenotypic selection regions during domestication, and set a stage for future utilization of molecular marker assisted breeding in cotton breeding programs.
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http://dx.doi.org/10.3389/fpls.2021.817748DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8763314PMC
January 2022

Association between greenness and dyslipidemia in patients with coronary heart disease: A proteomic approach.

Ecotoxicol Environ Saf 2022 Feb 15;231:113199. Epub 2022 Jan 15.

Department of Epidemiology and Health Statistics, School of Public Health, Fujian Medical University, Fuzhou 350122, China. Electronic address:

Background: Residential surrounding greenness may be protective of dyslipidemia are often theorized but remain poorly quantified. In particular, the underlying biological mechanisms of blood lipid changes with green spaces remain unclear.

Methods: Our observational epidemiology study included a residentially stable sample of 1035 coronary heart disease patients, and proteomics study included 16 participants. Normalized Difference Vegetation Index (NDVI) was used to evaluate residential greenness exposures. Proteomics technology was used to identify plasma greenness-related proteome disturbance, and the pathway analysis was employed to evaluate the potential biological mechanisms of greenness decreasing dyslipidemia risk.

Result: Higher residential surrounding greenness in the 500-m area was associated with lower risks of dyslipidemia (odds ratio (OR) = 0.871, 95% confidence interval (CI): 0.763, 0.994 for per one-quartile NDVI increase). Lymphocytes mediated 18.7% of the association between greenness and dyslipidemia. Greenness related proteins (including PLXDC1, IGFBP2 and LY6D) may regulate the biological functions of lipid metabolism and transport-related proteins (including ADIPOQ and CES1) through a series of biological processes.

Conclusion: People in greener surroundings have a lower risk of dyslipidemia, which may be due to their lower inflammation, stronger lipid transporter activity, and normal cholesterol metabolism.
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http://dx.doi.org/10.1016/j.ecoenv.2022.113199DOI Listing
February 2022

Value of M2BP in predicting in-stent restenosis in patients after coronary drug-eluting stent implantation.

Clin Cardiol 2022 Mar 15;45(3):308-314. Epub 2022 Jan 15.

Department of Cardiology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, China.

Objective: We evaluated the association between plasma levels of mac-2 binding protein (M2BP) with the risk of in-stent restenosis (ISR) after percutaneous coronary intervention (PCI).

Methods: Plasma M2BP levels were compared between 258 patients who experienced ISR at 12-months post-PCI and 258 patients, matched for age and sex, without angiographic evidence of ISR.

Results: The plasma M2BP level was significantly higher in the ISR than in the non-ISR group. On multivariate analysis, adjusted for potential clinical, biochemical, and angiography characteristics, M2BP remained as an independent significant predictor of ISR.

Conclusions: M2BP may be an important predictive biomarker of ISR and may be useful in identifying at-risk patients.
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http://dx.doi.org/10.1002/clc.23775DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8922530PMC
March 2022

Two-dimensional correlation spectroscopy indicates the infrared spectral markers of the optimum scorching degree of rhubarb (Rhei Radix et Rhizoma) to enhance the anti-inflammatory activity.

Spectrochim Acta A Mol Biomol Spectrosc 2022 Apr 5;270:120853. Epub 2022 Jan 5.

School of Life Sciences, Beijing University of Chinese Medicine, Beijing 102488, China. Electronic address:

Rhubarb has been used as herbal purgative with a long and worldwide history. But purgation is a side effect of rhubarb in many cases. In traditional Chinese medicine, rhubarb can be stir-baked to scorch to attenuate the purgative function while enhance other bioactivities such as the anti-inflammatory effect. However, the over-scorched rhubarb will lose necessary active ingredients and even generate toxic ingredients. Nowadays, the scorching degree of rhubarb is still determined by the intuitive observation of color changes in production. Therefore, this research was designed to develop more reasonable and objective criteria to evaluate the scorching degree to ensure the efficacy, safety and consistency of the scorched rhubarb. Taking the example of the rhubarb baked at 200 °C for different times, the combination of trait indicator (color), chemical indicator (combined and free AQs, sennosides, gallic acid, 5-HMF) and biological indicator (anti-inflammatory) showed that the rhubarb baked for 30 min can be treated as the right scorched. Two-dimensional correlation spectroscopy helped to reveal the infrared spectral markers of the water extract near 1694 cm, 1442 cm and 825 cm, as well as the relative strength of the absorption bands of the powder near 1610 cm and 1020 cm, to discriminate the right-scorched and over-scorched rhubarb. In conclusion, the combination of trait, chemical and biological indicators can provide reasonable and objective criteria for the optimum scorching degree of rhubarb, while FTIR spectroscopy can assess the right endpoint of the scorching process in a rapid, cheap and green way.
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http://dx.doi.org/10.1016/j.saa.2022.120853DOI Listing
April 2022

Construction of a novel filamentous fungal protein expression system based on redesigning of regulatory elements.

Appl Microbiol Biotechnol 2022 Jan 12;106(2):647-661. Epub 2022 Jan 12.

State Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Provincial Key Laboratory for Microbial Molecular Biology, College of Life Science, Hunan Normal University, Changsha, China.

Filamentous fungi are extensively used as an important expression host for the production of a variety of essential industrial proteins. They have significant promise as an expression system for protein synthesis due to their inherent superior secretory capabilities. The purpose of this study was to develop a novel expression system by utilizing a Penicillium oxalicum strain that possesses a high capacity for protein secretion. The expression of glycoside hydrolases in P. oxalicum was evaluated in a cleaner extracellular background where the formation of two major amylases was inhibited. Four glycoside hydrolases (CBHI, Amy15B, BGL1, and Cel12A) were expressed under the highly constitutive promoter PubiD. It was found that the proteins exhibited high purity in the culture supernatant after cultivation with starch. Two inducible promoters, Pamy15A and PempA, under the activation of the transcription factor AmyR were used as elements in the construction of versatile vectors. When using the cellobiohydrolase CBHI as the extracellular quantitative reporter, the empA promoter screened from the AmyR-overexpressing strain was shown to be superior to the amy15A promoter based on RNA-sequencing data. Therefore, we designed an expression system consisting of a cleaner background host strain and an adjustable promoter. This system enables rapid and high-throughput evaluation of glycoside hydrolases from filamentous fungi.Key points• A new protein expression system derived from Penicillium oxalicum has been developed.• The expression platform is capable of secreting recombinant proteins with high purity.• The adjustable promoter may allow for further optimization of recombinant protein synthesis.
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http://dx.doi.org/10.1007/s00253-022-11761-0DOI Listing
January 2022

Transcriptomic Analysis Reveals That Rho GTPases Regulate Trap Development and Lifestyle Transition of the Nematode-Trapping Fungus .

Microbiol Spectr 2022 02 12;10(1):e0175921. Epub 2022 Jan 12.

State Key Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Universitygrid.440773.3, Kunming, People's Republic of China.

Nematode-trapping (NT) fungi can form unique infection structures (traps) to capture and kill free-living nematodes and, thus, can play a potential role in the biocontrol of nematodes. Arthrobotrys oligospora is a representative species of NT fungi. Here, we performed a time course transcriptome sequencing (RNA-seq) analysis of transcriptomes to understand the global gene expression levels of A. oligospora during trap formation and predation. We identified 5,752 unique differentially expressed genes, among which the gene was significantly upregulated. Alternative splicing events occurred in 2,012 genes, including the and gene. Furthermore, we characterized three Rho GTPases (Rho2, Rac, and Cdc42) in A. oligospora using gene disruption and multiphenotypic analysis. Our analyses showed that AoRac and AoCdc42 play an important role in mycelium growth, lipid accumulation, DNA damage, sporulation, trap formation, pathogenicity, and stress response in A. oligospora. AoCdc42 and AoRac specifically interacted with components of the Nox complex, thus regulating the production of reactive oxygen species. Moreover, the transcript levels of several genes associated with protein kinase A, mitogen-activated protein kinase, and p21-activated kinase were also altered in the mutants, suggesting that Rho GTPases might function upstream from these kinases. This study highlights the important role of Rho GTPases in A. oligospora and provides insights into the regulatory mechanisms of signaling pathways in the trap morphogenesis and lifestyle transition of NT fungi. Nematode-trapping (NT) fungi are widely distributed in terrestrial and aquatic ecosystems. Their broad adaptability and flexible lifestyles make them ideal agents for controlling pathogenic nematodes. Arthrobotrys oligospora is a model species employed for understanding the interaction between fungi and nematodes. Here, we revealed that alternative splicing events play a crucial role in the trap development and lifestyle transition in A. oligospora. Furthermore, Rho GTPases exert differential effects on the growth, development, and pathogenicity of A. oligospora. In particular, AoRac is required for sporulation and trap morphogenesis. In addition, our analysis showed that Rho GTPases regulate the production of reactive oxygen species and function upstream from several kinases. Collectively, these results expand our understanding of gene expression and alternative splicing events in A. oligospora and the important roles of Rho GTPases in NT fungi, thereby providing a foundation for exploring their potential application in the biocontrol of pathogenic nematodes.
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http://dx.doi.org/10.1128/spectrum.01759-21DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8754127PMC
February 2022

Dual Targeting of Angipoietin-1 and von Willebrand Factor by microRNA-671-5p Attenuates Liver Angiogenesis and Fibrosis.

Hepatol Commun 2022 Jan 11. Epub 2022 Jan 11.

Department of Cell Biology, Municipal Laboratory for Liver Protection and Regulation of Regeneration, Capital Medical University, Beijing, China.

Angipoietin-1 (Angpt1) and von Willebrand factor (VWF) are two important angiogenic molecules that can drive pathologic angiogenesis and progression of liver fibrosis in our previous study. MicroRNAs (miRs) participate in a variety of physiological and pathological processes, including angiogenesis. However, the critical miRs targeting Angpt1 or VWF and potential molecular mechanism underlying liver fibrosis-associated angiogenesis is not clear yet. Human liver tissues were obtained from patients with different chronic liver diseases. Mouse models of liver fibrosis were induced by injection of CCl or bile duct ligation (BDL) operation. MiR-671-5p was predicted to target Angpt1 and VWF from three databases (miRanda, RNA22v2, and miRwalk). MiR-671-5p expression was decreased in the fibrotic liver of human and mice, with a negative correlation with the levels of Angpt1, VWF, sphingosine kinase-1 (SphK1, the rate-limiting enzyme for sphingosine 1-phosphate [S1P] formation), transforming growth factor β1 (TGFβ1), hypoxia inducible factor (Hif)1α, Hif2α, and fibrosis markers. Importantly, miR-671-5p expression was down-regulated in fluorescence-activated cell sorted liver sinusoidal endothelial cells and hepatic stellate cells (HSCs) in CCl mice compared with control mice. In vitro miR-671-5p expression was also decreased in S1P-stimulated HSCs and TGFβ1-activated liver sinusoidal endothelial cells, negatively correlated with Angpt1 and VWF expression. MiR-671-5p directly targeted Angpt1 and VWF by luciferase reporter assays. In vivo administration of miR-671-5p agomir decreased the messenger RNA and protein levels of Anpgt1 and VWF, and attenuated CCl -induced or BDL-induced liver angiogenesis and fibrosis. Conclusion: We identify the negative regulation of miR-671-5p on Angpt1 and VWF and liver fibrosis-associated angiogenesis, which may provide promising targets for the prevention and treatment of liver disease.
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http://dx.doi.org/10.1002/hep4.1888DOI Listing
January 2022

Place of Residence and Cognitive Function in Older Adults in China: The Mediating Role of Social Participation.

Int J Environ Res Public Health 2021 12 21;19(1). Epub 2021 Dec 21.

School of Health Humanities, Peking University, 38 Xueyuan Road, Beijing 100191, China.

Cognitive impairment is a severe health problem faced by older adults and their families, as well as the countries in which they live. Differences in place of residence may contribute to differences in the cognitive function of older adults, and the mediating effect of social participation has rarely been studied in China. A total of 10,014 older adult participants were included, using data from the Chinese Longitudinal Healthy Longevity Survey (CLHLS). Place of residence was described as either a city, town, or rural area. The frequency of participation in organized social activities and visits and interactions with friends was used to assess both formal and informal social participation. The Chinese version of a Mini-Mental State Examination (MMSE) was used as a measure of cognitive function. The mediation analysis was conducted using Hayes' process version 3.4 on SPSS (IBM, Armonk, NY, USA). Place of residence had a negative effect on cognitive function in older adults. The mediating functions of both informal (a1b1 = 0.199) and formal (a2b2 = -0.056) social participation indicate a suppression effect on the part of informal social participation and a partial mediation effect on the part of formal social participation in terms of the association between place of residence and cognitive function in older adults. Promoting both informal and formal social participation seems to be an important strategy for preventing a decline in the cognitive function of older adults, especially for those living in rural areas.
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http://dx.doi.org/10.3390/ijerph19010013DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8750759PMC
December 2021

Effects of sheep slaughter age on myogenic characteristics in skeletal muscle satellite cells.

Anim Biosci 2022 Apr 3;35(4):614-623. Epub 2022 Jan 3.

College of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot 010018, China.

Objective: The objective of this study was to investigate the effects of sheep slaughter age on myogenic characteristics in skeletal muscle satellite cells (SMSCs).

Methods: Primary SMSCs were isolated from hind leg biceps femoris muscles of Wurank lambs (slaughtered at three months, Mth-3) and adults (slaughtered at fifteen months, Mth-15). SMSCs were selected by morphological observation and fluorescence staining. Myogenic regulatory factors (MRF) and myosin heavy chain (MyHC) expressions of SMSCs were analyzed on days 1, 3, 4, and 5.

Results: The expressions of myogenic factor 5 (Myf5), myogenic differentiation (MyoD), Myf6, and myogenin (MyoG) in Mth-15 were significantly higher in Mth-15 than in Mth-3 on days 1, 3, and 4 (p<0.05). However, MyoG expression in Mth-15 was significantly lower than in Mth-3 on day 5 (p<0.05). The expressions of MyHC I, MyHC IIa, and MyHC IIx in Mth-15 were significantly higher than in Mth-3 on days 1 and 3 (p<0.05), and MyHC IIb were significantly lower than in Mth-3 on days 3 and 4 (p<0.05). In contrast, the expression of MyHC IIx in Mth-15 was significantly lower and MyHC IIb was significantly higher than in Mth-3 on days 5 (p<0.05).

Conclusion: The slaughter age altered the expression of MRFs and MyHCs in SMSCs while differentiation, which caused the variation of myogenic characteristics, and thus may affect the meat quality of Wurank sheep.
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http://dx.doi.org/10.5713/ab.21.0193DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8902214PMC
April 2022

Association between social capital and sleep duration among rural older adults in China.

BMC Public Health 2022 01 5;22(1):12. Epub 2022 Jan 5.

School of Management, Shanxi Medical University, 56 Xinjian South Road, Taiyuan, Shanxi Province, China.

Objective: Sleep disturbances are great challenges to older adults' health promotion. The study tested gender differences in the association between different dimensions of social capital and self-reported sleep duration of Chinese rural older adults.

Design: The data of rural older adults were extracted from a national cross-sectional survey of the Chinese Longitudinal Healthy Longevity Survey (CLHLS) and analyzed in this study.

Setting: CLHLS covered 23 provinces in China.

Participants: The 6552 rural respondents aged ≥65 years old were involved.

Main Outcome Measures: Generalized trust, informal social participation, formal social participation and social support were used to assess social capital. Self-reported sleep duration was measured as health outcome.

Results: Low level of generalized trust had harmful effect on insufficient sleep (AOR 1.110, 95% CI 1.018-1.324), and having no formal or informal social participation was significantly positively associated with long sleep (AOR 1.424, 95% CI 1.007-2.013; AOR 1.241, 95% CI 1.016-1.516). Rural older female adults with no emotional social support had higher odds of insufficient sleep (AOR 1.502, 95% CI 1.258-1.978). Meanwhile, both informal and formal social participation showed inverse association with long sleep for females.

Conclusions: This study found the relationship between social capital, sleep duration and the gender differences in Chinese rural older adults. More targeted sleep disturbance interventions could be taken in social capital of rural older adults, and gender differences should be considered when making social capital-embedded health promotion policies and interventions.
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http://dx.doi.org/10.1186/s12889-021-12441-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8734274PMC
January 2022

Effect of Neurorepair for Motor Functional Recovery Enhanced by Total Saponins From Maxim. Treatment in a Rat Model of Focal Ischemia.

Front Pharmacol 2021 10;12:763181. Epub 2021 Dec 10.

School of Traditional Chinese Medicine, Capital Medical University, Beijing, China.

Maxim. (TTM), is a perennial herb from Liliaceae, that has been widely used as a traditional Chinese medicine treating cephalgia and traumatic hemorrhage. The present work was designed to investigate whether the total saponins from Maxim. (TSTT) would promote brain remodeling and improve gait impairment in the chronic phase of ischemic stroke. A focal ischemic model of male Sprague-Dawley (SD) rats was established by permanent middle cerebral artery occlusion (MCAO). Six hours later, rats were intragastrically treated with TSTT (120, 60, and 30 mg/kg) and once daily up to day 30. The gait changes were assessed by the CatWalk-automated gait analysis system. The brain tissues injuries, cerebral perfusion and changes of axonal microstructures were detected by multimodal magnetic resonance imaging (MRI), followed by histological examinations. The axonal regeneration related signaling pathways including phosphatidylinositol 3-kinases (PI3K)/protein kinase B (AKT)/glycogen synthase kinase-3 (GSK-3)/collapsin response mediator protein-2 (CRMP-2) were measured by western blotting. TSTT treatment significantly improved gait impairment of rats. MRI analysis revealed that TSTT alleviated tissues injuries, significantly improved cerebral blood flow (CBF), enhanced microstructural integrity of axon and myelin sheath in the ipsilesional sensorimotor cortex and internal capsule. In parallel to MRI findings, TSTT preserved myelinated axons and promoted oligodendrogenesis. Specifically, TSTT interventions markedly up-regulated expression of phosphorylated GSK-3, accompanied by increased expression of phosphorylated PI3K, AKT, but reduced phosphorylated CRMP-2 expression. Taken together, our results suggested that TSTT facilitated brain remodeling. This correlated with improving CBF, encouraging reorganization of axonal microstructure, promoting oligodendrogenesis and activating PI3K/AKT/GSK-3/CRMP-2 signaling, thereby improving poststroke gait impairments.
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http://dx.doi.org/10.3389/fphar.2021.763181DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8703076PMC
December 2021
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