Publications by authors named "Le Wang"

978 Publications

Nocardioides lacusdianchii sp. nov., an attached bacterium of Microcystis aeruginosa.

Antonie Van Leeuwenhoek 2021 Nov 30. Epub 2021 Nov 30.

College of Pharmacy and Life Science, Jiujiang University, Jiujiang, 332000, People's Republic of China.

Attached bacteria of Microcystis play important roles in the occurence, outbreak and decline of Microcystis water blooms. In this study, a novel actinobacterium, designated strain JXJ CY 38, was isolated from the culture mass of Microcystis aeruginosa FACHB-905 (MAF), collected from Lake Dianchi, south-west, China. The strain was found to be a Gram-stain positive, short rod, catalase positive and oxidase negative. The isolate was found to be able to grow at 5.0-38.0 °C (optimum, 28.0 °C), pH 4.0-11.0 (optimum, 7.0-8.0) and 0-3.0% (w/v, optimum, 0%) NaCl. Based on 16S rRNA gene sequences, strain JXJ CY 38 shows high similarities to Nocardioides furvisabuli JCM 13813 (99.0%) and Nocardioides alpinus JCM 18960 (98.7%), and less than 98.2% similarities to other members of the genus. The major cellular fatty acids (> 10.0%) were identified as iso-C (23.6%), Cω9c (18.2%) and Cω8c (16.4%), while the predominant menaquinone was found to be MK-8 (H). The diagnostic diamino acids in the cell wall peptidoglycan were identified as aspartic acid, glutamic acid, glycine and alanine, with mannose, ribose and arabinose as whole cell sugars. The polar lipids were found to be diphosphatidylglycerol, phosphatidylglycerol, a phospholipid, phosphatidylcholine and an unidentified lipid. The DNA G + C content was determined to be 71.3%. The digital DNA-DNA hybridization and average nucleotide identity values between strain JXJ CY 38 and the type strains N. furvisabuli JCM 13813 and N. alpinus JCM 18960 were 49.4% and 37.7%, and 92.0% and 83.4%, respectively. On the basis of the above taxonomic data and differences in physiological characteristics from the closely related type strains, strain JXJ CY 38 was determined to represent a novel species of genus Nocardioides, for which the name Nocardioides lacusdianchii sp. nov. is proposed. The type strain is JXJ CY 38 (= KCTC 49381 = CGMCC 4.7665). Strain JXJ CY 38 apparently exhibits complex effects on the interactions between MAF and other attached bacteria, including the promotion or inhibition of the growth of MAF and bacteria, and the synthesis and release of microcystins by MAF.
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http://dx.doi.org/10.1007/s10482-021-01690-9DOI Listing
November 2021

Identification of serum exosomal miR-98-5p, miR-183-5p, miR-323-3p and miR-19b-3p as potential biomarkers for glioblastoma patients and investigation of their mechanisms.

Curr Res Transl Med 2021 Nov 24;70(1):103315. Epub 2021 Nov 24.

Departments of VIP Unit, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130033, China. Electronic address:

Background: Exosomal miRNAs have attracted increasing interest as potential biomarkers and treatment targets for cancers, however, glioblastoma (GBM)-related exosomal miRNAs remain rarely reported. The study aimed to screen crucial serum exosomal miRNAs in GBM patients and explored their possible mechanisms.

Methods: Serum exosomal miRNA profile datasets of GBM patients and normal controls were downloaded from the Gene Expression Omnibus database (GSE112462 and GSE122488). The differentially expressed miRNAs (DEMs) were identified using the limma method. Their diagnostic values were assessed by receiver operating characteristic (ROC) curve analysis. The target genes of DEMs were predicted by the miRwalk 2.0 database. Function enrichment analysis was performed using the DAVID database. The expression and prognosis of target genes were validated using TCGA sequencing data and immunohistochemistry.

Results: Seven DEMs were shared in two datasets, among which hsa-miR-183-5p and hsa-miR-98-5p as well as has-miR-323-3p or has-miR-19b-3p constituted a diagnostic signature to distinguish GBM from controls, with the area under the ROC curve nearly approximate to 1. MAPK8IP1/FAM175B, OSMR/CASP3, PTPN2 and FBXO32 may be underlying targets for hsa-miR-183-5p, hsa-miR-98-5p, has-miR-323-3p and has-miR-19b-3p, respectively. Function analysis showed all of these target genes were involved in cell proliferation and related signaling pathways [positive regulation of cell proliferation (OSMR), negative regulation of transcription from RNA polymerase II promoter (PTPN2), cell division (FAM175B), regulation of transcription, DNA-templated (MAPK8IP1), hsa05200:Pathways in cancer (CASP3) and hsa04068:FoxO signaling pathway (FBXO32)]. The protein and (or mRNA) expression levels of OSMR, CASP3, PTPN2 and FBXO32 were validated to be upregulated, while MAPK8IP1 and FAM175B were downregulated in GBM tissues. Also, OSMR, CASP3, PTPN2 and FBXO32 were associated with patients' prognosis.

Conclusion: These findings suggest these four exosomal miRNAs may represent potential diagnostic biomarkers and therapeutic targets for GBM.
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http://dx.doi.org/10.1016/j.retram.2021.103315DOI Listing
November 2021

Sex Differences in Dopamine Receptor Signaling in Knockout Mice: A Pilot Study.

Brain Sci 2021 Oct 24;11(11). Epub 2021 Oct 24.

Campbell Family Mental Health Research Institute, Centre for Addiction and Mental Health, Toronto, ON M5T 1R8, Canada.

Fragile X syndrome (FXS) is an X-chromosome-linked dominant genetic disorder that causes a variable degree of cognitive dysfunction and developmental disability. Current treatment is symptomatic and no existing medications target the specific cause of FXS. As with other X-linked disorders, FXS manifests differently in males and females, including abnormalities in the dopamine system that are also seen in -knockout (KO) mice. We investigated sex differences in dopamine signaling in -KO mice in response to L-stepholidine, a dopamine D1 receptor agonist and D2 receptor antagonist. We found significant sex differences in basal levels of phosphorylated protein kinase A (p-PKA) and glycogen synthase kinase (GSK)-3β in wild type mice that were absent in -KO mice. In wild-type mice, L-stepholidine increased p-PKA in males but not female mice, decreased p-GSK-3 in female mice and increased p-GSK-3 in male mice. Conversely, in -KO mice, L-stepholidine increased p-PKA and p-GSK-3β in females, and decreased p-PKA and p-GSK-3β in males.
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http://dx.doi.org/10.3390/brainsci11111398DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8615700PMC
October 2021

Occurrence of total mercury and methylmercury in rice: Exposure and health implications in Nepal.

Ecotoxicol Environ Saf 2021 Nov 22;228:113019. Epub 2021 Nov 22.

State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang 550081, China.

Emerging studies have indicated that the consumption of rice could be the major methylmercury (MeHg) contributor to human mercury (Hg) exposure. Nonetheless, few studies are available on Hg in rice around the world, especially in countries with heavy rice diet. In this study, total Hg (THg) and MeHg levels in rice samples (n = 172) across Nepal were first investigated. The geometric mean THg was 7.05 ± 7.71 µg/kg with a range of 0.622 µg/kg to 158 µg/kg, and the maximum THg level was up to 791% of the Chinese National Standard Limit for THg in rice (20 µg/kg). The geometric mean MeHg was 0.820 ± 0.660 µg/kg with a range of 0.189 µg/kg to 8.59 µg/kg. Overall, the mean MeHg exposure (0.00445 ± 0.00477 µg/kg bw/day) and inorganic Hg (IHg) exposure (0.0360 ± 0.0739 µg/kg bw/day) were lower than the reference dose (RfD) of 0.1 µg/kg bw/day for MeHg and the provisional tolerable weekly intake (PTWI) of 0.571 µg/kg bw/day for IHg, respectively. Concerning different groups of vulnerable populations, the highest MeHg exposure (0.126 µg/kg bw/day) and IHg exposure (1.57 µg/kg bw/day) of preschoolers (37-50 months old) were approximately 126% of the RfD for MeHg and 275% of the PTWI for IHg. When the pregnant mothers eat the rice without awareness of the Hg content in rice, the mean and highest intelligence quotients (IQs) losses were 9554 and 118659 points, respectively, and the corresponding economic costs due to IQ loss could be 15.1 million USD and 188 million USD in Nepal. The results of rice THg and MeHg levels and corresponding exposure in populations highlighted the occurrence of rice THg and MeHg pollution issues in Nepal. More efforts should be made to protect younger groups in Nepal from high rice Hg exposure. CAPSULE: Owing to the high rice consumption rates relative to body mass, preschoolers (37-50 months) may meet the 126% reference dose (0.1 µg/kg bw/day) for MeHg and 275% provisional tolerable weekly intake (0.571 µg/kg bw/day) for IHg exposure in Nepal.
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http://dx.doi.org/10.1016/j.ecoenv.2021.113019DOI Listing
November 2021

Proton pump inhibitors may reduce the risk of high-grade dysplasia and/or esophageal adenocarcinoma in Barrett's esophagus: a systematic review and meta-analysis.

Expert Rev Clin Pharmacol 2021 Nov 27:1-10. Epub 2021 Nov 27.

Department of Gastroenterology, General Hospital of Northern Theater Command (formerly called General Hospital of Shenyang Military Area), Shenyang, China.

Background: Barrett's esophagus (BE) is an important risk factor for high-grade dysplasia (HGD) and/or esophageal adenocarcinoma (EAC). The effect of proton pump inhibitors (PPIs) on the chemoprevention of HGD and/or EAC arising from BE remains controversial.

Research Design And Methods: PubMed, EMBASE, and Cochrane Library databases were systematically searched. Risk ratios (RRs) or odds ratios (ORs) with 95% confidence intervals (CIs) were pooled by a random-effect model. Heterogeneity and its potential source were assessed.

Results: Fifteen studies with 26,291 BE patients were included. Meta-analysis of eight cohort studies showed that PPIs can significantly reduce the risk of HGD and/or EAC in BE patients (RR = 0.46; P < 0.001), but meta-analysis of six case-control studies showed no significant benefit of PPIs (OR = 0.64; P = 0.334). Heterogeneity was significant among both cohort and case-control studies, which might be attributed to the information sources of PPIs. There was no significant protective effect of high-dose PPIs on HGD and/or EAC in one RCT (RR = 0.84; P = 0.21), meta-analysis of two cohort studies (RR = 0.61; P = 0.28), or meta-analysis of two case-control studies (OR = 0.32; P = 0.08).

Conclusions: Chemoprevention of HGD and/or EAC by PPIs may be considered in BE patients. However, there might not be further preventive effect of high-dose PPIs.
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http://dx.doi.org/10.1080/17512433.2022.2008909DOI Listing
November 2021

A single intronic single nucleotide polymorphism in splicing site of steroidogenic enzyme is associated with phenotypic sex in oyster pompano, .

Proc Biol Sci 2021 Nov 17;288(1963):20212245. Epub 2021 Nov 17.

Molecular Population Genetics Group, Temasek Life Sciences Laboratory, Singapore 117604, Singapore.

Teleosts show varied master sex determining (MSD) genes and sex determination (SD) mechanisms, with frequent turnovers of sex chromosomes. Tracing the origins of MSD genes and turnovers of sex chromosomes in a taxonomic group is of particular interest in evolutionary biology. Oyster pompano (), a marine fish, belongs to the family Carangidae, in which () has repeatedly evolved to an MSD gene. Whole-genome resequencing identified a single nucleotide polymorphism (SNP) at chromosome 24 to be strictly associated with phenotypic sex, with females being the heterozygous sex. This SNP is located in a splicing site at the first exon/intron boundary of . The Z-linked SNP results in malfunction of all spliced isoforms, whereas the W-linked isoforms were predicted to have open reading frames that are conserved among vertebrates, suggesting that is a female-determining gene. The differential alternative splicing patterns of ZZ and ZW genotypes were consistently observed both in undifferentiated stages and differentiated gonads. We observed elevated recombination around the SD locus and no differentiation between Z and W chromosomes. The extreme diversity of mutational mechanisms that evolves to an MSD gene highlights frequent turnovers between sex chromosomes in the Carangidae.
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http://dx.doi.org/10.1098/rspb.2021.2245DOI Listing
November 2021

Eco-Friendly and Efficient Luminescent Solar Concentrators Based on a Copper(I)-Halide Composite.

ACS Appl Mater Interfaces 2021 Dec 16;13(47):56348-56357. Epub 2021 Nov 16.

State Key Laboratory of Reliability and Intelligence of Electrical Equipment, Hebei University of Technology, 5340 Xiping Road, Tianjin 300401, P. R. China.

Luminescent solar concentrators (LSCs) show great promise in reducing the cost of silicon solar cells due to their potential use for high-efficiency energy harvesting. Compared to narrow absorption organic dyes, quantum dots (QDs) are a favorable approach to acquire stable LSCs. However, the use of toxic heavy metals in QDs and the small Stokes shift largely restrict their development. Here, a toxic metal-free, highly luminescent ink based on a copper(I)-halide hybrid cluster is reported, whose quantum yield (QY) exceeds 68%. Under the interaction with halohydrocarbon, CuI and phenethylamine (PEA) can be easily dissolved and the ink can be facilely acquired. The obtained film exhibits strong orange light emission with a large Stokes shift. As a proof-of-concept experiment, (PEA)CuI has been used to fabricate LSCs. The as-prepared LSC (4 cm × 4 cm × 0.3 cm) exhibits an internal quantum efficiency () as high as 44.1%. After coupling to a solar cell, an optical conversion efficiency () of 6.85% is acquired from this LSC. In addition, the LSC possesses high stability such as air stability, water stability, and photostability. These results demonstrate that the (PEA)CuI film can be employed as a promising candidate for large-area and high-efficiency LSCs.
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http://dx.doi.org/10.1021/acsami.1c18361DOI Listing
December 2021

The first complete mitochondrial genome of the Grey-bellied Green Ratsnake (Gray, 1853) and its phylogenetic placement.

Mitochondrial DNA B Resour 2021 10;6(12):3348-3349. Epub 2021 Nov 10.

Ministry of Ecology and Environment, Nanjing Institute of Environmental Sciences, Nanjing, China.

We sequenced the complete 17,209 bp mitochondrial genome (mitogenome) of (Squamata: Colubridae) using next-generation sequencing. It consists of 13 PCGs, two ribosomal RNA genes, 22 transfer RNA genes, one non-coding region of an L-strand replication origin, and two control regions. The overall nucleotide composition was 34.7% of A, 24.8% of T, 12.3% of G, and 28.1% of C. The result of the phylogenetic analysis showed that , a member of Colubridae, is sister to other New World and Old World ratsnakes. The new data could help better understand the phylogenetic status of the genus and the evolutionary history of Colubridae species.
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http://dx.doi.org/10.1080/23802359.2021.1959436DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8583752PMC
November 2021

Corrigendum to 'High mobility group box 1 antibody represses autophagy and alleviates hippocampus damage in pilocarpine-induced mouse epilepsy model' [Acta Histochemica 122 (2020) 151485].

Acta Histochem 2021 Nov 11:151815. Epub 2021 Nov 11.

Department of Neurology, Qilu Hospital of Shandong University, Jinan, Shandong 250012, People's Republic of China. Electronic address:

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http://dx.doi.org/10.1016/j.acthis.2021.151815DOI Listing
November 2021

Clinical determinants of relapse following CAR-T therapy for hematologic malignancies: Coupling active strategies to overcome therapeutic limitations.

Authors:
Le Wang

Curr Res Transl Med 2021 Nov 8;70(1):103320. Epub 2021 Nov 8.

The advent of chimeric antigen receptor (CAR)-T cell therapy has been hailed as a major breakthrough in the treatment of B cell acute lymphoblastic leukemia (B-ALL) and diffuse large B-cell lymphoma (DLBCL). While multiple promising CAR-T cell clinical trials continue to receive approval from the FDA and the Chinese Clinical Trial Register (ChiCTR), many hematologic malignancies patients nonetheless experience disease relapse following treatment as a consequence of genetic mutations, antigen escape, lineage switching, poor CAR-T cell persistence, CAR T cell exhaustion, and immunogenicity against CAR T cells. In this article, we summarize the structural characteristics of CAR constructs and discuss clinical factors known to be related to relapse following CAR-T cell treatment. By better understanding the mechanistic basis for such disease recurrence, it will be possible to fully realize the potential of this potent therapeutic modality in the future. This review will focus on current activate strategies aimed at overcoming known limitations to CAR-T cell therapy in an effort to improve hematologic malignancies patient outcomes.
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http://dx.doi.org/10.1016/j.retram.2021.103320DOI Listing
November 2021

Pulmonary sarcomatoid carcinoma mimicking pleural mesothelioma: A case report.

Medicine (Baltimore) 2021 Nov;100(45):e27813

Department of Respiratory and Critical Care Medicine, Tianjin Medical University General Hospital, Tianjin, China.

Introduction: Pulmonary sarcomatoid carcinoma (PSC) is an extremely rare biphasic tumor characterized by a mixture of malignant epithelial and mesenchymal cells. Owing to the rarity, as well as the lack of typical manifestations and imaging signs, the rate of misdiagnosis is high. Herein, we present a case of PSC misdiagnosed as pleural mesothelioma in a 59-year-old man.

Patient Concerns: A 59-year-old man presented with recurrent coughing, fever, and chest pain.

Diagnosis: Chest computed tomography showed 2 large and dense masses involving the inferior lobes of right lung, along with slight irregular pleural thickening and a small amount of effusion.

Interventions: Chest computed tomography-guided tumor biopsy was performed. PSC was confirmed based on histopathology and immunohistochemistry. The patient refused treatment due to economic reasons.

Outcomes: The patient developed adrenal, multiple lung and brain metastasis. The overall survival time was 11 months.

Conclusions: PSC, despite its rarity, should be considered in the differential diagnosis of lung cancer. Besides, biopsy, histopathology, and specific immunohistochemical staining of larger tissue specimens can be contributing to the accurate diagnosis of PSC.
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http://dx.doi.org/10.1097/MD.0000000000027813DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8589231PMC
November 2021

Effect of different cooking water on flavor characteristics of mutton soup.

Food Sci Nutr 2021 Nov 7;9(11):6047-6059. Epub 2021 Sep 7.

China Meat Research Centre Beijing China.

The mutton flavor is affected by cooking water significantly, and the flavor of mutton is delicious and widely loved by consumers through an extremely simple processing in northwest China, such as Inner Mongolia, Ningxia, and Xinjiang. The flavor shows obvious changes if get out of these areas even use the same raw meat, which may be caused by different cooking water. To determine whether and how the cooking water affect the mutton soup flavor, the elements in water, the flavor was studied by inductively coupled plasma mass spectrometry (ICP-MS), amino acid analyzer, and thermal desorption (TDS)-gas chromatography-mass spectrometry (GC-MS). Specifically, three water samples from different sources, Ningxia (NXW), Beijing (BJW), and ultrapure water from the laboratory (PUW), were used for cooking with Tan sheep's ribs to get different mutton soups. The inductively coupled plasma mass spectrometry (ICP-MS) results showed that the elements and the concentration of solutes in different water sources were significantly different. The NXW batch had the highest Na, Mg, K, and Sr concentrations, and Na in NXW water reached to 50.60 mg/L, which existed as Na, significantly ( < .05) higher than BJW (8.63 mg/L) and PUW, which were important to the flavor of mutton soup. The PUW batch had the highest content of free amino acids, and the content of glutamic acid (Glu) reached to 17.89 μg/mL. The NXW batch had the highest content of taste nucleotides, and the content of 5´-IMP reached to 68.68 μg/ml. The volatiles of the three batches had significant differences, and only 40 volatiles were detected in all batches. Further flavor studies using electronic nose and electronic tongue showed significant differences in overall aroma and overall taste, especially bitterness, saltiness, and astringency. The results could provide a basis for improving the flavor quality for the mutton soup.
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http://dx.doi.org/10.1002/fsn3.2546DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8565249PMC
November 2021

Pretreatment of Sulfonylureas Reducing Perihematomal Edema in Diabetic Patients With Basal Ganglia Hemorrhage: A Retrospective Case-Control Study.

Front Neurol 2021 22;12:736383. Epub 2021 Oct 22.

Department of Neurology, Xijing Hospital, Fourth Military Medical University (Air Force Medical University), Xi'an, China.

The sulfonylurea receptor 1-transient receptor potential melastatin 4 (SUR1-TRPM4) channel is a target key mediator of brain edema. Sulfonylureas (SFUs) are blockers of the SUR1-TRPM4 channel. We made two assessments for the pretreatment of SFUs: (1) whether it associates with lower perihematomal edema (PHE) and (2) whether it associates with improved clinical outcomes in diabetic patients who have acute basal ganglia hemorrhage. This retrospective case-control study was conducted in diabetic adults receiving regular SFUs before the onset of intracerebral hemorrhage (ICH). All of the patients received the clinical diagnosis of spontaneous basal ganglia hemorrhage. The diagnosis was confirmed by a CT scan within 7 days after hemorrhage. For each case, we selected two matched controls with basal ganglia hemorrhage based on admission time (≤5 years) and age differences (≤5 years), with the same gender and similar hematoma volume. The primary outcome was PHE volume, and the secondary outcomes were relative PHE (rPHE), functional independence according to modified Rankin Scale score and Barthel Index at discharge, and death rate in the hospital. A total of 27 patients (nine cases and 18 matched controls), admitted between January 1, 2009 and October 31, 2018, were included in our study. There was no significant association between SFU patients and non-SFU patients on PHE volumes [15.4 (7.4-50.2 ml) vs. 8.0 (3.1-22.1) ml, = 0.100]. Compared to non-SFU patients, the SFU patients had significantly lower rPHE [0.8 (0.7-1.3) vs. 1.5 (1.2-1.9), = 0.006]. After we adjusted the confounding factors, we found that sulfonylureas can significantly reduce both PHE volume (regression coefficient: -13.607, 95% CI: -26.185 to -1.029, = 0.035) and rPHE (regression coefficient: -0.566, 95% CI: -0.971 to -0.161, = 0.009). However, we found no significant improvement in clinical outcomes at discharge, in the event of pretreatment of SFUs before the onset of ICH, even after we adjusted the confounding factors. For diabetic patients with acute basal ganglia hemorrhage, pretreatment of sulfonylureas may associate with lower PHE and relative PHE on admission. No significant effect was found on the clinical outcomes when the patients were discharged. Future studies are needed to assess the potential clinical benefits using sulfonylureas for ICH patients.
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http://dx.doi.org/10.3389/fneur.2021.736383DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8569795PMC
October 2021

Budget-constraint admissible output consensus tracking for intermittent-interaction singular multiagent networks.

ISA Trans 2021 Oct 25. Epub 2021 Oct 25.

Rocket Force University of Engineering, Xi'an, 710025, PR China.

Budget-constraint admissible output consensus tracking design and analysis for the intermittent-interaction singular multiagent networks with given cost budgets are addressed in this paper. Two critical characteristics of many practical multiagent networks are focused. The first one is that the dynamics of each agent contains dynamical modes, as well as impulsive modes and static modes, and interactions among agents are intermittent. The second one is that the network is constrained by the cost budget, and the weighted design between tracking performances and energy consumptions should be guaranteed under budget constraints. Firstly, in virtue of the decomposition of observability, a new singular consensus tracking protocol with the dynamic output feedback is proposed by intermittent protocol states. Meanwhile, a cost index is constructed by considering both the tracking performance and the energy consumption. Then, criteria of budget-constraint admissible output consensus tracking analysis and design are given. By designing the gain matrices of consensus tracking protocols, not only multiagent networks with dynamical modes and static modes reach output consensus tracking while eliminating impulsive modes, but also the upper bound of the cost index can be kept within the cost budget; that is, the weighted design with the budget constraint is achieved. Finally, theoretical results are demonstrated by numerical simulations.
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http://dx.doi.org/10.1016/j.isatra.2021.10.021DOI Listing
October 2021

Single-molecule real-time sequencing of the full-length transcriptome of purple garlic (Allium sativum L. cv. Leduzipi) and identification of serine O-acetyltransferase family proteins involved in cysteine biosynthesis.

J Sci Food Agric 2021 Nov 5. Epub 2021 Nov 5.

State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining, China.

Background: Garlic (Allium sativum L.), whose bioactive components are mainly organosulfur compounds (OSCs), is a herbaceous perennial widely consumed as a green vegetable and a condiment. Yet, the metabolic enzymes involved in the biosynthesis of OSCs are not identified in garlic.

Results: Here, a full-length transcriptome of purple garlic was generated via PacBio and Illumina sequencing, to characterize the garlic transcriptome and identify key proteins mediating the biosynthesis of OSCs. Overall, 22.56 Gb of clean data were generated, resulting in 454 698 circular consensus sequence (CCS) reads, of which 83.4% (379 206) were identified as being full-length non-chimeric reads - their further transcript clustering facilitated identification of 36 571 high-quality consensus reads. Once corrected, their genome-wide mapping revealed that 6140 reads were novel isoforms of known genes, and 2186 reads were novel isoforms from novel genes. We detected 1677 alternative splicing events, finding 2902 genes possessing either two or more poly(A) sites. Given the importance of serine O-acetyltransferase (SERAT) in cysteine biosynthesis, we investigated the five SERAT homologs in garlic. Phylogenetic analysis revealed a three-tier classification of SERAT proteins, each featuring a serine acetyltransferase domain (N-terminal) and one or two hexapeptide transferase motifs. Template-based modeling showed that garlic SERATs shared a common homo-trimeric structure with homologs from bacteria and other plants. The residues responsible for substrate recognition and catalysis were highly conserved, implying a similar reaction mechanism. In profiling the five SERAT genes' transcript levels, their expression pattern varied significantly among different tissues.

Conclusion: This study's findings deepen our knowledge of SERAT proteins, and provide timely genetic resources that could advance future exploration into garlic's genetic improvement and breeding. © 2021 Society of Chemical Industry.
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http://dx.doi.org/10.1002/jsfa.11627DOI Listing
November 2021

Fluorescent and Antibacterial Aminobenzeneboronic Acid (ABA)-Modified Gold Nanoclusters for Self-Monitoring Residual Dosage and Smart Wound Care.

ACS Nano 2021 Nov 1. Epub 2021 Nov 1.

Shenzhen Key Laboratory of Smart Healthcare Engineering, Department of Biomedical Engineering, Southern University of Science and Technology, No. 1088 Xueyuan Road, Nanshan District, Shenzhen, Guangdong 518055, China.

The replacement of dressings may cause secondary damage to the wounds; thus, the real-time monitoring of the state of wound dressings is crucial for evaluating wound care processes. Herein, we report a smart dressing to self-monitor residue nanomedicine on it during the application. We load aminobenzeneboronic acid (ABA)-modified gold nanoclusters (A-GNCs) on bacterial cellulose (BC) membranes as an antibacterial wound dressing to display the amount of residual nanomedicine (A-GNCs) by colorimetry during the application in remedying multi-drug-resistant (MDR) bacteria-infected wounds. A-GNCs emit bright orange fluorescence under UV light, whereas the BC membrane is transparent at a humidified state on the wounds. Thus, the BC-A-GNCs nanocomposite (BGN) shows decreasing intensity of orange fluorescence with the release of the A-GNCs, indicating the appropriate time points for the replacement of the dressing. The BGN, which can realize accurate self-monitoring in a simple, low-cost, and efficient way, thus holds great promise for broad clinical applications.
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http://dx.doi.org/10.1021/acsnano.1c06139DOI Listing
November 2021

Unique functional responses of fungal communities to various environments in the mangroves of the Maowei Sea in Guangxi, China.

Mar Pollut Bull 2021 Oct 26;173(Pt B):113091. Epub 2021 Oct 26.

Key Laboratory of Marine Chemistry Theory and Technology (Ocean University of China), Ministry of Education, Qingdao 266100, Shandong, PR China; College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 266100, Shandong, PR China. Electronic address:

Fungi are important compartments of microbial communities of mangroves. Their diversity might be influenced by their habitat environment. This study analyzed the distribution and function of fungal communities in the sediments and plant samples from mangrove ecosystem of the Maowei Sea area in Guangxi, China. The results showed that phytopathogenic fungi Cladosporium (17.00%) was mainly observed in the sediments from the protected zone, while endophytic fungi Alternaria (9.22%) and Acremonium (6.09%) were only observed in the sediments from wharf. The fungi in the sediments from village and park were mainly consisted of high-activity endophytes and fungi related to lignin-degrading, respectively. Acaulospora and Aspergillus with higher relative abundance discovered in plant tissues could help plant growth. Cirrenalia (37.66%) and Lignincola (26.73%) with high-activity for lignin-degrading were discovered in decayed leaves. The distribution and function of fungi were highly dependent on the environment settings, thus the fungi can be used as indicators for monitoring the environmental change of mangrove ecosystems.
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http://dx.doi.org/10.1016/j.marpolbul.2021.113091DOI Listing
October 2021

CBX2 Induces Glioma Cell Proliferation and Invasion Through the Akt/PI3K Pathway.

Technol Cancer Res Treat 2021 Jan-Dec;20:15330338211045831

117865Tianjin Medical University General Hospital, Tianjin, China.

Glioma is the most common primary intracranial tumor. Abnormal expression of CBX2 (ChromoBox2) is associated with tumorigenesis and tumor development. TCGA data in UALCAN showed that CBX2 was overexpressed in glioma tissue. To confirm the role of CBX2 in glioma, we regulated the level of CBX2 and conducted colony formation, Transwell, and CCK-8 assays to verify the effect of CBX2. The results showed that CBX2 knockdown reduced glioma cell proliferation and invasion and that the cells were less tumorigenic. CBX2 overexpression induced glioma cell proliferation and invasion and glioma stem cell self-renewal. The animal experiments showed that CBX2 knockdown inhibited glioma growth and improved survival time. CBX2 knockdown inhibited activation of the Akt/PI3K pathway. epidermal growth factor rescued the effects of CBX2. CBX2 could induce the growth and invasion of glioma cells via the Akt/PI3K pathway.
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http://dx.doi.org/10.1177/15330338211045831DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8558802PMC
October 2021

Analyses of serum and urinary metabolites in individuals with peripheral artery disease (PAD) consuming a bean-rich diet: Relationships with drug metabolites.

Appl Physiol Nutr Metab 2021 Oct 26. Epub 2021 Oct 26.

University of Manitoba, 8664, Winnipeg, Manitoba, Canada, R3T 2N2;

Peripheral artery disease (PAD) has high morbidity and mortality rates. A metabolomics approach was employed to determine whether consumption of bean-rich diets for 8 weeks would impact the metabolomic profile of PAD individuals. Serum and urine, collected from 54 participants with clinical PAD at baseline and after 8 weeks on 0.3 cups beans/d (n=19), 0.6 cups beans/d (n= 20), or control (n=23) diet, and the beans were extracted and analyzed using LC-QTOF-MS. As a result, PGE2 p-acetamidophenyl ester, PGF2α diethyl amide and 5-L-glutamyl-L-alanine were significantly changed in the serum or urine of bean groups compared to control. Significant changes (P<0.05) in the profile and/or levels of 22 flavonoids present in bean extracts showed the potential importance of the mixture of beans used in this study. In a subset of participants taking metoprolol, after 8 weeks the bean-rich diets significantly elevated metoprolol in the serum while reducing it in urine compared to baseline. In addition, the diets significantly enhanced the urinary excretion of metformin. In conclusion, several biochemical pathways including prostaglandins and glutathione were affected by bean consumption. Significant changes in the metabolism of metoprolol and metformin with bean consumption suggested the presence of diet-drug interactions that may require adjustment of the prescribed dose. ClinicalTrials.gov Identifier: NCT01382056 Novelty: • Bean consumption by people with PAD alters the levels of certain metabolites in serum and urine • Different bean types (black, red kidney, pinto, navy) have unique flavonoid profiles • Metabolomics revealed potential diet-dug interactions as serum and/or urinary levels of metoprolol and metformin are modified by bean consumption.
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http://dx.doi.org/10.1139/apnm-2021-0495DOI Listing
October 2021

Organocatalytic Direct Asymmetric Indolization from Anilines by Enantioselective [3 + 2] Annulation.

Org Lett 2021 Nov 26;23(21):8434-8438. Epub 2021 Oct 26.

We report the efficient syntheses of chiral tetrahydroindole pyrazolinones by the asymmetric [3 + 2] cascade cyclizations (indolizations) of simple aniline derivatives with pyrazolinone ketimines as 2C synthons. The chiral phosphoric-acid-catalyzed system uses a concerted π-π interaction/dual H-bond control strategy to catalytically direct the asymmetric aniline, which undergoes a highly chemo-, regio-, and enantioselective [3 + 2] cascade annulation, furnishing a series of optically active tetra-hydroindole pyrazolinones with two contiguous chiral aza-quaternary carbon centers in excellent yields with excellent enantioselectivities. This method features a relatively broad substrate scope for amines and 2-naphthylamines and highlights the emerging value of direct chiral indolizations from simple amine sources in organic synthesis.
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http://dx.doi.org/10.1021/acs.orglett.1c03162DOI Listing
November 2021

Prognostic Significance of Preprocedural N-Terminal Pro-B-Type Natriuretic Peptide Assessment in Diabetic Patients With Multivessel Coronary Disease Undergoing Revascularization.

Front Cardiovasc Med 2021 6;8:721260. Epub 2021 Oct 6.

Department of Cardiology, Tianjin Chest Hospital, Tianjin, China.

The N-terminal pro-B-type natriuretic peptide (NT-proBNP) may predict adverse cardiovascular outcomes in patients with diabetes. However, its prognostic value in patients with multivessel disease (MVD) undergoing coronary revascularization remains unclear. This study aimed to evaluate the prognostic significance of preprocedural NT-proBNP levels in diabetic patients with MVD undergoing coronary revascularization. A total of 886 consecutive diabetic patients with MVD who underwent coronary revascularization were enrolled in this study. Patients were divided into quartiles according to their pre-procedural NT-proBNP levels. Kaplan-Meier curves and Cox regression analyses were performed to evaluate the risk of cardiovascular events, including all-cause death, cardiovascular death, myocardial infarction (MI), stroke, and major adverse cardiovascular events (MACE), according to the NT-proBNP quartiles. During a median follow-up period of 4.2 years, 111 patients died (with 82 being caused by cardiovascular disease), 133 had MI, 55 suffered from stroke, and 250 experienced MACE. Kaplan-Meier curves demonstrated that NT-proBNP levels were significantly associated with higher incidences of all-cause death, cardiovascular death, MI, and MACE (log-rank test, < 0.001, respectively). Multivariate Cox regression analysis revealed that NT-proBNP level was an independent predictor of adverse outcomes, including all-cause death (HR, 1.968; 95% CI, 1.377-2.812; < 0.001), cardiovascular death (HR, 1.940; 95% CI, 1.278-2.945; = 0.002), MI (HR, 1.722; 95% CI, 1.247-2.380; = 0.001), and MACE (HR, 1.356; 95% CI, 1.066-1.725; = 0.013). The role of NT-proBNP in predicting adverse outcomes was similar in patients with stable angina pectoris and acute coronary syndrome. Moreover, preprocedural NT-proBNP alone discriminated against the SYNTAX II score for predicting all-cause death [area under the curve (AUC), 0.662 vs. 0.626, = 0.269], cardiovascular death (AUC, 0.680 vs. 0.622, = 0.130), MI (AUC, 0.641 vs. 0.579, = 0.050), and MACE (AUC, 0.593 vs. 0.559, = 0.171). The addition of NT-proBNP to the SYNTAX II score showed a significant net reclassification improvement, integrated discrimination improvement, and improved C-statistic (all < 0.05). NT-proBNP levels were an independent prognostic marker for adverse outcomes in diabetic patients with MVD undergoing coronary revascularization, suggesting that preprocedural NT-proBNP measurement might help in the risk stratification of high-risk patients.
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http://dx.doi.org/10.3389/fcvm.2021.721260DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8526556PMC
October 2021

Contributions and characteristics of two bifunctional GH43 β-xylosidase /α-L-arabinofuranosidases with different structures on the xylan degradation of Paenibacillus physcomitrellae strain XB.

Microbiol Res 2021 Dec 4;253:126886. Epub 2021 Oct 4.

College of Life Sciences, Capital Normal University, Beijing, 100048, China. Electronic address:

Xylan is one of the major polymeric hemicellulosic constituents of lignocellulosic biomass, and its effective utilization by microorganisms is crucial for the economical production of biofuels. In this study, Paenibacillus physcomitrellae XB exhibited different xylan degradation ability on different substrates of corncob xylan (CCX), oat spelt xylan (OSX), wheat flour arabinoxylan (AX) and beech wood xylan (BWX). The RT-QPCR result showed that two genes (Pph_0602 and Pph_2344) belonging to the glycoside hydrolase family 43 were up-regulated more than 5-fold on CCX and xylose. Substrate-specific assays with purified proteins Ppxyl43A (Pph_0602) and Ppxyl43B (Pph_2344) revealed that both exhibited β-xylosidase activity toward the chromogenic substrate p-nitrophenyl-β-D-xylopyranoside, and α-L-arabinofuranosidase activity toward p-nitrophenyl-α-L-arabinofuranoside, indicating their bifunctionality. By testing their degradation characteristics on different natural substrates, it was found that both Ppxyl43A and Ppxyl43B showed similar degradation ability on CCX and OSX. Both enzymes could hydrolyze xylohexaose and xylobiose completely to xylose, but could not hydrolyze BWX and AX, suggesting they mainly hydrolyze xylo-oligosaccharides by β-xylosidase activity. Further analysis showed that both of them displayed very high pH stability and thermostability on the β-xylosidase activity, but Ppxy143B exhibited wider pH and temperature ranges, higher pH and temperature stability, was less influenced by metal ions, and had a slower start-up response than Ppxyl43A. Given their predicted structure, it is likely that the enzymatic differences between Ppxyl43A and Ppxyl43B might be related to the extra C-terminus domain (GH43_C2) in Ppxyl43B, which could enhance the enzymatic stability while restricting the substrates' or metal ions' access to the active sites of Ppxyl43B. In conclusion, both Ppxyl43A and Ppxyl43B were β-xylosidase/α-L-arabinofuranosidase bifunctional enzymes and might be useful in xylan biomass conversion, especially in the hydrolysis of xylo-oligosaccharides into xylose.
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http://dx.doi.org/10.1016/j.micres.2021.126886DOI Listing
December 2021

Enhancing Dye-Triplet-Sensitized Upconversion Emission Through the Heavy-Atom Effect in CsLu F :Yb/Er Nanoprobes.

Angew Chem Int Ed Engl 2021 Oct 22. Epub 2021 Oct 22.

CAS Key Laboratory of Design and Assembly of Functional Nanostructures, Fujian Key Laboratory of Nanomaterials, State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian, 350002, China.

Lanthanide (Ln )-doped upconversion (UC) nanoprobes, which have drawn extensive attention for various bioapplications, usually suffer from small absorption cross-sections and weak luminescence intensity of Ln ions. Herein, we report the controlled synthesis of a new class of Ln -doped UC nanoprobes based on CsLu F :Yb/Er nanocrystals (NCs), which can effectively increase the intersystem crossing (ISC) efficiency from singlet excited state to triplet excited state of IR808 up to 99.3 % through the heavy atom effect. By virtue of the efficient triplet sensitization of IR808, the optimal UC luminescence (UCL) intensity of IR808-modified CsLu F :Yb/Er NCs is enhanced by 1309 times upon excitation at 808 nm. Benefiting from the intense dye-triplet-sensitized UCL, the nanoprobes are demonstrated for sensitive assay of extracellular and intracellular hypochlorite with an 808-nm/980-nm dual excited ratiometric strategy.
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http://dx.doi.org/10.1002/anie.202112125DOI Listing
October 2021

Therapeutic time windows of compounds against NMDA receptors signaling pathways for ischemic stroke.

J Neurosci Res 2021 Oct 21. Epub 2021 Oct 21.

Department of Pharmacy, Hebei University of Science and Technology, Shijiazhuang, China.

Much evidence has proved that excitotoxicity induced by excessive release of glutamate contributes largely to damage caused by ischemia. In view of the key role played by NMDA receptors in mediating excitotoxicity, compounds against NMDA receptors signaling pathways have become the most promising type of anti-stroke candidate compounds. However, the limited therapeutic time window for neuroprotection is a key factor preventing NMDA receptor-related compounds from showing efficacy in all clinical trials for ischemic stroke. In this perspective, the determination of therapeutic time windows of these kinds of compounds is useful in ensuring a therapeutic effect and accelerating clinical application. This mini-review discussed the therapeutic time windows of compounds against NMDA receptors signaling pathways, described related influence factors and the status of clinical studies. The purpose of this review is to look for compounds with wide therapeutic time windows and better clinical application prospect.
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http://dx.doi.org/10.1002/jnr.24937DOI Listing
October 2021

Isolation of Novel Mycobacterium Species from Skin Infection in an Immunocompromised Person.

Emerg Infect Dis 2021 11;27(11):2944-2947

We investigated a case of cutaneous infection in an immunocompromised patient in China that was caused by a novel species within the Mycobacterium gordonae complex. Results of whole-genome sequencing indicated that some strains considered to be M. gordonae complex are actually polyphyletic and should be designated as closely related species.
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http://dx.doi.org/10.3201//eid2711.210426DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8544998PMC
November 2021

Prediction of Readmission in Geriatric Patients From Clinical Notes: Retrospective Text Mining Study.

J Med Internet Res 2021 10 19;23(10):e26486. Epub 2021 Oct 19.

Ng Teng Fong General Hospital, Singapore, Singapore.

Background: Prior literature suggests that psychosocial factors adversely impact health and health care utilization outcomes. However, psychosocial factors are typically not captured by the structured data in electronic medical records (EMRs) but are rather recorded as free text in different types of clinical notes.

Objective: We here propose a text-mining approach to analyze EMRs to identify older adults with key psychosocial factors that predict adverse health care utilization outcomes, measured by 30-day readmission. The psychological factors were appended to the LACE (Length of stay, Acuity of the admission, Comorbidity of the patient, and Emergency department use) Index for Readmission to improve the prediction of readmission risk.

Methods: We performed a retrospective analysis using EMR notes of 43,216 hospitalization encounters in a hospital from January 1, 2017 to February 28, 2019. The mean age of the cohort was 67.51 years (SD 15.87), the mean length of stay was 5.57 days (SD 10.41), and the mean intensive care unit stay was 5% (SD 22%). We employed text-mining techniques to extract psychosocial topics that are representative of these patients and tested the utility of these topics in predicting 30-day hospital readmission beyond the predictive value of the LACE Index for Readmission.

Results: The added text-mined factors improved the area under the receiver operating characteristic curve of the readmission prediction by 8.46% for geriatric patients, 6.99% for the general hospital population, and 6.64% for frequent admitters. Medical social workers and case managers captured more of the psychosocial text topics than physicians.

Conclusions: The results of this study demonstrate the feasibility of extracting psychosocial factors from EMR clinical notes and the value of these notes in improving readmission risk prediction. Psychosocial profiles of patients can be curated and quantified from text mining clinical notes and these profiles can be successfully applied to artificial intelligence models to improve readmission risk prediction.
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http://dx.doi.org/10.2196/26486DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8564665PMC
October 2021

Natural diterpenoid eriocalyxin B covalently modifies glutathione and selectively inhibits thioredoxin reductase inducing potent oxidative stress-mediated apoptosis in colorectal carcinoma RKO cells.

Free Radic Biol Med 2021 Dec 14;177:15-23. Epub 2021 Oct 14.

Shaanxi Key Laboratory of Phytochemistry and College of Chemistry and Chemical Engineering, Baoji University of Arts and Sciences, Baoji, 721013, China. Electronic address:

Increasing evidence suggests the significant contribution of high levels of thioredoxin reductase (TrxR) in various stages of tumorigenesis and resistance to tumor chemotherapy. Thus, inhibition of TrxR with small molecules is an attractive strategy for cancer therapy. Eriocalyxin B (EriB), a naturally occurring diterpenoid extracted from Isodon eriocalyx, has reflected potential anticancer activities through numerous pathways. Here, we describe that EriB covalently modifies GSH and selectively inhibits TrxR activity by targeting the Sec residue of the enzyme. Pharmacological inhibition of TrxR by EriB results in elevated ROS levels, reduced total GSH and thiols content, which ultimately induced potent RKO cell apoptosis mediated by oxidative stress. Importantly, EriB indicates potent synthetic lethality with GSH inhibitors, BSO, in RKO cells. In summary, our results highlight that targeting TrxR by EriB explores a novel mechanism for the biological action of EriB. This opened up a new therapeutic indication for using EriB to combat cancers.
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http://dx.doi.org/10.1016/j.freeradbiomed.2021.10.013DOI Listing
December 2021

Impact of persistent subclinical hypothyroidism on clinical outcomes in non-ST-segment elevation acute coronary syndrome undergoing percutaneous coronary intervention.

Clin Endocrinol (Oxf) 2021 Oct 12. Epub 2021 Oct 12.

The Eighth Department of Cardiology, Tianjin Chest Hospital, Tianjin, China.

Background: Data on the association of subclinical hypothyroidism (SCH) with the severity of coronary artery disease and major adverse cardiovascular and cerebral events (MACCE) in patients with non-ST-segment elevation acute coronary syndrome (NSTE-ACS) after percutaneous coronary intervention (PCI) are limited and conflicting.

Objective: We established the baseline rate of SCH and followed the trajectory of thyroid-stimulating hormone (TSH) values during and after hospitalisation for PCI for up to six months and determined whether persistent SCH was associated with the severity of coronary artery disease and MACCE in patients with NSTE-ACS after PCI.

Design: Population-based prospective cohort study.

Patients: We included patients with NSTE-ACS who underwent PCI with simple balloon angioplasty or stent implantation for coronary heart disease.

Measurements: Thyroid function tests of patients before PCI and 1 day, 1 week, 1 and 6 months after PCI were performed. Cases showing transient SCH were excluded. Patients were divided into two groups based on the results of four TSH tests: 0.27-4.2 mIU/L (n = 1472, 89.7%) and >4.2 mIU/L (n = 170, 10.4%). The risk factors for the severity of coronary artery lesions were estimated using multinomial logistic regression analysis. Univariate and multivariate Cox regression analyses were used to study the relationship between TSH and MACCE.

Results: Among 1642 patients, there were 1070 males (65.2%) and 572 females (34.8%), with an average age of 62.5 ± 9.6 years. SCH patients had a wider range of diseased vessels and a higher number of diseased vessels (p < .05). TSH level was an independent risk factor for moderate [odds ratio (OR) = 1.144, 95% confidence interval (95% CI): 1.057-1.237, p = .001] and severe (OR = 1.131, 95% CI: 1.043-1.226, p = .003) coronary artery lesions. After adjusting for covariates, the risk of MACCE [hazard ratio (HR): 4.067, p < .001], nonfatal myocardial infarction (HR: 14.724, p = .003), and unplanned PCI (HR: 5.028, p < .001) were higher in the SCH group than in the euthyroidism group. There were no significant differences in the incidence of heart failure (HR: 6.012, p = .175), nonfatal stroke (HR: 2.039, p = .302), unplanned coronary artery bypass grafting (CABG) (HR: 1.541, p = .57), or cardiac death (HR: 2.704, p = .375) between the two groups.

Conclusions: Preoperative TSH levels and changes in thyroid hormone levels several months post-PCI in NSTE-ACS patients are highly significant in practice. Persistent SCH is associated with severe coronary artery lesions and MACCE, and may be a predictor for evaluating the prognosis of PCI-treated NSTE-ACS patients.
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http://dx.doi.org/10.1111/cen.14613DOI Listing
October 2021

Polymorphonuclear neutrophils promote endothelial apoptosis by enhancing adhesion upon stimulation by intermittent hypoxia.

Sleep Breath 2021 Oct 11. Epub 2021 Oct 11.

Department of Respiratory and Critical Care Medicine, Tianjin Medical University General Hospital, 154 Anshan Road, Heping District, Tianjin, 300052, China.

Purpose: This study explored the interactive effects between polymorphonuclear neutrophils (PMNs) and vascular endothelial cells under intermittent hypoxia (IH) and investigated the mechanisms underlying these effects.

Methods: Endothelial cells were co-cultured with PMNs isolated from rats exposed to normoxia or IH. The PMN apoptotic rate was determined using flow cytometry. Expression of apoptosis-related proteins in the endothelial cells were evaluated using Western blotting, and the levels of intercellular adhesion molecules in the co-culture supernatants were measured using enzyme-linked immunosorbent assay.

Results: The PMN apoptotic rate in the IH-exposed rat group was significantly lower than that of the normoxia control group. There was a positive relationship between the PMN apoptotic rate and IH exposure time. In endothelial cells co-cultured with PMNs isolated from IH-exposed rats, a significant increase in the protein expression levels of Bax, Bcl-2, and caspase-3 and a significant decrease in the Bcl-2/Bax ratio were observed. Furthermore, the intercellular cell adhesion molecule-1(ICAM-1) and E-select element (E-S) levels were elevated significantly in the co-cultured supernatants of endothelial cells and PMNs from IH-exposed rats compared to that from controls. The above IH-induced alterations were partially restored by tempol pretreatment.

Conclusions: The apoptotic rate was low in PMNs from IH-exposed rats, which consequently increased the apoptotic signals in endothelial cells in vitro. This may be associated with the increased levels of intercellular adhesion molecules. Further, tempol partially attenuates the PMN-mediated pro-apoptotic effects on endothelial cells under IH.
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http://dx.doi.org/10.1007/s11325-021-02503-zDOI Listing
October 2021

Overexpression of DTL enhances cell motility and promotes tumor metastasis in cervical adenocarcinoma by inducing RAC1-JNK-FOXO1 axis.

Cell Death Dis 2021 10 11;12(10):929. Epub 2021 Oct 11.

Key laboratory of preservation of human genetic resources and disease control in China (Harbin Medical University), Ministry of Education, Harbin, Heilongjiang, 150081, China.

Cervical adenocarcinoma is an important disease that affects young women and it has a high mortality and poor prognosis. Denticleless E3 ubiquitin protein ligase homolog (DTL) gene with oncogenic function has been evaluated in several cancers. Through this study, we aimed to clarify the clinical and molecular characteristics of cervical adenocarcinoma involving overexpression of DTL and elucidate its molecular mechanism. Bioinformatics analysis was performed through multiple databases. RNA sequencing was used to obtain differentially expressed genes after DTL was overexpressed in cells. The role of DTL in cervical adenocarcinoma was explored through in vitro and in vivo experiments. We found that DTL has an unfavorable prognostic implication for patients with cervical adenocarcinoma. Overexpression of DTL induced the migration and invasion of tumor cells in vitro and promoted intra-pulmonary metastasis in vivo. In addition, DTL activated JNK through RAC1 and upregulated FOXO1 to induce epithelial-mesenchymal transition, and the migration and invasion of tumor cells. Therefore, we conclude that overexpression of DTL enhanced cell motility and promoted tumor metastasis of cervical adenocarcinoma by regulating the RAC1-JNK-FOXO1 axis. These results suggest that DTL may become a potential therapeutic target for antitumor metastasis of cervical adenocarcinoma.
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http://dx.doi.org/10.1038/s41419-021-04179-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8505428PMC
October 2021
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