Publications by authors named "Laura D"

25 Publications

  • Page 1 of 1

Investigation of the Interactions of Cationic Guar with Human Hair by Electrokinetic Analysis.

Int J Cosmet Sci 2021 Apr 3. Epub 2021 Apr 3.

Objective: Cationic guar is an important polysaccharide used as a hair conditioning agent in personal care products. In this article, we report streaming potential data demonstrating its behavior as it interacts electrostatically with hair. Several cationic guar variants with different molecular weights (MWs) and charge densities (CDs) were examined.

Methods: All experiments were carried out with a custom-designed streaming potential instrument so that in situ, real-time data were monitored during the treatment of a hair plug with aqueous solutions of cationic guar and subsequent treatment with anionic surfactants-sodium laureth sulfate (SLES) and cocamidopropyl betaine (CAPB)-commonly found in contemporary shampoo formulations.

Results: The MW of the cationic guar variants plays an integral role in determining the thickness of the adsorbed polymer layer on the hair surface while CD influences the zeta potential. Data were also generated for the treatment of hair with a cationic flexible polymer (polyquaternium-28) and cationic conditioning surfactant (behentrimonium chloride) to provide a frame of reference. The deposition behavior on hair of high MW cationic guar variants is distinct from these conventional molecules in terms of its electrokinetic properties. We also examined the electrokinetic behavior of cationic guar on hair types from different racial backgrounds. While the cationic guar treatment yielded similar results for the different hair types, anionic surfactant treatment resulted in quicker sorption and desorption from African, European 65% gray, and Mulatto hair as compared to Chinese, European dark brown, and Indian hair.

Conclusion: We introduce an in-situ technique for measuring the dynamic sorption/desorption of charged molecules on the surface of human hair. Evaluation of a series of cationic guar species revealed varying behavior depending on the MW and CD of the polysaccharide. Our data also demonstrate differences in the desorption properties of typical shampoo surfactants for hair from diverse racial backgrounds.
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http://dx.doi.org/10.1111/ics.12704DOI Listing
April 2021

Cost-Effectiveness Analysis of Rivaroxaban Plus Aspirin Compared with Aspirin Alone in Patients with Coronary and Peripheral Artery Diseases in Italy.

Clin Drug Investig 2021 Mar 16. Epub 2021 Mar 16.

Center for Public Health Research, University of Milano-Bicocca, Via Cadore 48, 20900, Monza, Italy.

Background: Rivaroxaban is a selective inhibitor of coagulation factor Xa and its combination with aspirin showed better outcomes in the prevention of recurrent cardiovascular disease than aspirin alone.

Objective: This analysis aimed to economically compare the cost effectiveness of rivaroxaban (2.5 mg twice daily) plus aspirin (100 mg once daily) with aspirin alone in patients with coronary artery disease (CAD) or peripheral artery disease (PAD) and related subgroups.

Methods: The analysis simulates the perspective of the Italian National Healthcare Service and used a state-transition decision Markov model. Clinical efficacy data and health events risks were gathered from the COMPASS trial. Health outcomes and costs (in Euros) were evaluated over a lifetime horizon and were discounted at 3.5% per annum. Direct healthcare costs entered the analysis. Results were expressed in terms of incremental cost-effectiveness ratio (ICER), defined as cost per quality-adjusted life-year (QALY) gained. One-way deterministic and probabilistic sensitivity analyses were performed.

Results: For the CAD or PAD population, rivaroxaban plus aspirin was more effective and costly compared with aspirin alone. Incremental costs and efficacy produced an ICER of €16,522 per QALY gained. Analyses found similar trends for the PAD and CAD groups, with respective ICERs of €8003 and €18,599, while ICERs for the other groups were lower than €13,000 per QALY. Sensitivity analyses confirmed these findings.

Conclusion: Compared with aspirin alone, rivaroxaban plus aspirin is cost effective in preventing recurrent cardiovascular events in all patients with CAD or PAD, from the Italian perspective. These results could help clinicians and decision makers to develop improved strategies for cardiovascular disease prevention.
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http://dx.doi.org/10.1007/s40261-021-01023-8DOI Listing
March 2021

Stress granules in Ciona robusta: First evidences of TIA-1-related nucleolysin and tristetraprolin gene expression under metal exposure.

Comp Biochem Physiol C Toxicol Pharmacol 2021 May 17;243:108977. Epub 2021 Jan 17.

Department of Biology, University of Padova, Via Ugo Bassi 58/B, 35131 Padova, Italy.

Stress granules are non-membranous cytoplasmic foci, composed of non-translating messenger ribonucleoproteins, translational initiation factors and other additional proteins. They represent a primary mechanism to rapidly modulate gene expression when cells are subjected to adverse environmental conditions. Very few works have been devoted to study the presence of the molecular components of stress granules in invertebrates. In this work, we characterized the transcript sequences for two important protein components of stress granules, TIA-1-related nucleolysin (TIAR) and tristetraprolin (TTP), in the solitary ascidian Ciona robusta, an invertebrate chordate, and carried out the first studies on their gene expression under stress conditions induced by metals (Cu, Zn and Cd). Data on mRNA expression levels, provided by qRT-PCR analyses, show a generalized decrease at the second day of metal-exposure for both tiar and ttp, suggesting that metal accumulation induces acute stress and the inhibition of the transcription for the two studied proteins. In-situ hybridization analyses demonstrate that TIAR and TTP antisense riboprobes recognize circulating granular amoebocytes in the hemolymph, in both blood lacunae and tunic. The results obtained in this work increase our knowledge on the evolution of anti-stress proteins in metazoans and emphasize the importance of the transcription of tiar and ttp, which represents an efficient physiological response allowing organisms to survive in the environment under stress conditions.
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http://dx.doi.org/10.1016/j.cbpc.2021.108977DOI Listing
May 2021

Fundus Pigmentation in the Diagnosis and Treatment of Retinopathy of Prematurity.

Ophthalmology 2020 Dec 29. Epub 2020 Dec 29.

Department of Ophthalmology, Bascom Palmer Eye Institute, Miller School of Medicine, University of Miami, Miami, Florida. Electronic address:

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http://dx.doi.org/10.1016/j.ophtha.2020.12.024DOI Listing
December 2020

Ocular trauma secondary to exercise resistance bands during the COVID-19 pandemic.

Am J Emerg Med 2021 Apr 4;42:217-220. Epub 2020 Dec 4.

Bascom Palmer Eye Institute, University of Miami, Miami, FL, United States of America.

Objective: To characterize injuries caused by exercise resistance bands.

Method: Single-site retrospective case series of patients presenting to the Bascom Palmer Eye Institute emergency room with ocular injuries secondary to exercise resistance bands from March through September 2020.

Results: Eleven patients (9 males, 2 females, 14 eyes) were reviewed. Eight patients had a unilateral injury (3 right eyes, 5 left eyes) while 3 had bilateral injuries. Iritis was the most common presentation, seen in all 11 patients, followed by hyphema (9 patients, 82%), and vitreous hemorrhage (4 patients, 36%). Among affected eyes, the mean presenting visual acuity was approximately 20/100, improving to 20/40 on the last follow up (p = 0.06). However, 4 eyes (33%) had vision ≤20/60 at last follow up.

Conclusions: Exercise resistance bands can cause a wide spectrum of ocular injuries, some leading to long-term vision loss. As such, we recommend that patients strongly consider using eye protection goggles or glasses while using resistance bands for exercise.
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http://dx.doi.org/10.1016/j.ajem.2020.11.054DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7716731PMC
April 2021

Ocular Manifestations of Sarcoidosis in a South Florida Population.

Clin Ophthalmol 2020 2;14:3741-3746. Epub 2020 Nov 2.

Miami Veterans Administration Medical Center, Miami, FL 33125, USA.

Objective: To describe the ocular manifestations of sarcoidosis in a South Florida population and identify risk factors for the presence of ocular disease.

Design: Retrospective consecutive case series.

Methods: Medical charts of individuals with sarcoidosis seen in the University of Miami pulmonary department were reviewed for ocular disease. Odds ratios were used to identify risk factors for ocular sarcoidosis.

Results: Fourteen of 108 individuals with sarcoidosis had ocular involvement. The mean age of the 14 individuals was 56±15 years. Seventy-one percent were female, 50% were black, and 21% were Hispanic. Twelve had uveitis of which panuveitis was the most common subtype. Five had ≤20/70 vision in at least one eye due to uveitis. Neurosarcoidosis was a risk factor for ocular sarcoidosis (OR 6.14, p=0.03, 95% CI 1.21-31.09).

Conclusion: Ocular manifestations occurred in a minority of individuals in a pulmonary sarcoidosis clinic in South Florida. Uveitis was the most common ocular manifestation. Neurosarcoidosis was a risk factor for ocular involvement.
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http://dx.doi.org/10.2147/OPTH.S278373DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7648141PMC
November 2020

Intraocular Invasion of Ocular Surface Squamous Neoplasia Through a Corneal Wound.

Ophthalmol Ther 2020 Dec 31;9(4):1083-1088. Epub 2020 Aug 31.

Department of Ophthalmology, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL, USA.

Purpose: We report a case of intraocular invasion of ocular surface squamous neoplasia (OSSN) through a cataract surgery wound that manifested as an anterior chamber membrane, and perform a review of the literature.

Methods: An 87-year-old woman with history of an incompletely excised OSSN 10 days prior presented with decreased vision due to an anterior chamber membranous film that seemed connected to an old cataract wound. The membrane was biopsied, and histopathology revealed dysplastic squamous epithelial cells of conjunctival origin. Ten days later the tumor expanded to cover the entire iris surface, and a modified enucleation was performed.

Results: Histopathology revealed invasive OSSN extending deep to the ciliary body and laterally into the anterior chamber. The patient developed a submandibular node metastasis 7 months later. Literature review revealed six cases of invasive OSSN resulting from extension through a corneal wound, with varied presentations and outcomes.

Conclusion: Our case is the first to report invasive OSSN presenting as an anterior chamber membrane as a result of incomplete excision of an ocular surface malignancy adjacent to a corneal wound poses a risk for intraocular tumor extension and distant metastasis.
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http://dx.doi.org/10.1007/s40123-020-00294-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7708601PMC
December 2020

Widefield Fluorescein Angiography in the Fellow Eyes of Patients with Presumed Unilateral Persistent Fetal Vasculature.

Ophthalmol Retina 2021 03 26;5(3):301-307. Epub 2020 Jul 26.

Department of Ophthalmology, Bascom Palmer Eye Institute, University of Miami, Florida. Electronic address:

Purpose: To examine the retinal vascular findings on widefield fluorescein angiography (FA) in the fellow eyes of patients with unilateral persistent fetal vasculature (PFV).

Design: Retrospective case series.

Participants: Consecutive patients with unilateral PFV evaluated by a single physician at an academic medical center from February 1, 2011, to November 30, 2018.

Methods: Clinical and demographic information, including age, gender, race, ethnicity, affected eye, subtype, stalk origin, complications on presentation, length of follow-up, and examination findings, was reviewed using the electronic medical record. Fluorescein angiograms of the affected and fellow eyes were reviewed by 2 authors for characteristic retinal vascular abnormalities. Agreement between the authors' scores was analyzed using Cohen's Kappa.

Main Outcome Measures: Fluorescein angiography abnormalities, including peripheral vessel avascularity, aberrant circumferential vessels, terminal supernumerary branching, regional capillary dropout, terminal bulbing, abnormal choroidal flush, abnormal vessel straightening, and peripheral vessel leakage or dilation.

Results: Inclusion criteria were met by 41 patients. The average age at initial visit was 10.0 months. The average length of follow-up was 36.4 months. Abnormalities on FA were seen in 31 (75.6%) fellow eyes: peripheral vessel avascularity in 27.5 (67.1%), aberrant circumferential vessels in 20 (48.8%), terminal supernumerary branching in 17 (41.5%), regional capillary dropout in 9 (22.0%), terminal bulbing in 6 (14.6%), abnormal choroidal flush in 3.5 (8.5%), and abnormal vessel straightening and peripheral vessel leakage in 2.5 (6.1%). Statistical analysis disclosed an overall observed agreement of 93.4% among the raters (κ = 0.84, P < 0.0001).

Conclusions: Retinal vascular abnormalities seen in patients with unilateral PFV were present in the majority of fellow eyes. This suggests that unilateral PFV may in fact be a bilateral, asymmetric process, but the clinical significance of these subtle findings is not known.
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http://dx.doi.org/10.1016/j.oret.2020.07.020DOI Listing
March 2021

A unique case of coats plus syndrome and dyskeratosis congenita in a patient with CTC1 mutations.

Ophthalmic Genet 2020 08 16;41(4):363-367. Epub 2020 Jun 16.

Department of Ophthalmology, Bascom Palmer Eye Institute, Miller School of Medicine, University of Miami , Miami, FL, USA.

Coats plus syndrome (CP) is a rare condition characterized by bilateral exudative retinal telangiectasias with associated systemic disorders primarily affecting the brain, bone and gastrointestinal tract due to a mutation in the gene. mutations are also known to cause dyskeratosis congenita (DC), which is an inherited bone marrow failure syndrome characterized by skin pigmentation abnormalities, nail dystrophy, and oral leukoplakia. This is the first reported case of a patient diagnosed with both CP and DC caused by compound heterozygous gene mutations. Moreover, one of the variant mutations found in this patient has never been published before.
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http://dx.doi.org/10.1080/13816810.2020.1772315DOI Listing
August 2020

Genotypic and Phenotypic Antibiotic Resistance in Staphylococcus Epidermidis Endophthalmitis.

Ophthalmic Surg Lasers Imaging Retina 2020 05;51(5):S13-S16

Background And Objective: To evaluate antibiotic resistance patterns in Staphylococcus epidermis endophthalmitis isolates, identify antibiotic resistance genes, and compare this to their phenotypic resistance.

Materials And Methods: Retrospective case series of S. epidermidis isolates from January 2012 to December 2017. Phenotypic resistance was determined from minimum inhibitory concentration values. Genotypic resistance was determined from DNA microarray. The relationship between these was analyzed using Cohen's kappa and predictive value.

Results: Seventy-five isolates were included. More than 60% were resistant to methicillin and erythromycin. Approximately 60% possessed the resistance genes for methicillin and erythromycin, and 25% for clindamycin. There was near perfect agreement between genotype and phenotype for erythromycin and methicillin (kappa = 0.88 and 0.89, respectively). Approximately 98% (P < 0.0001) of isolates with erythromycin and methicillin resistance genes and 90% (P = 0.0006) with clindamycin resistance genes were phenotypically resistant to those antibiotics. None were resistant to vancomycin.

Conclusion: The relationship between genotypic and phenotypic antibiotic resistance highlights that resistance may be polygenic, and genes may be silent. [Ophthalmic Surg Lasers Imaging Retina. 2020;51:S13-S16.].
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http://dx.doi.org/10.3928/23258160-20200108-02DOI Listing
May 2020

Select pediatric vitreoretinal disease in the setting of Turner's syndrome.

Am J Ophthalmol Case Rep 2020 Jun 13;18:100662. Epub 2020 Mar 13.

Bascom Palmer Eye Institute, Department of Ophthalmology, University of Miami, 900 NW 17th Street, Miami, FL, 33136, USA.

Purpose: To report 2 cases of pediatric vitreoretinal disease in the setting of Turner's syndrome.

Observations: A 4-year-old girl with Turner's syndrome was referred for evaluation of a tractional retinal detachment in the right eye. Fundoscopic examination disclosed temporal dragging of the macula in the right eye, and vascular nonperfusion in the right and left eyes. Genetic testing revealed a novel frameshift mutation in the gene consistent with familial exudative vitreoretinopathy (FEVR). The patient was treated with laser. A 14-year-old girl with Turner's syndrome presented with nyctalopia. Dilated fundus exam disclosed peri-foveal pigmentary changes and peripheral bone spicules. Full-field electroretinography demonstrated decreased rod and cone responses, consistent with retinitis pigmentosa (RP).

Conclusions And Importance: Vitreoretinal disease, including RP and FEVR, is rarely observed in patients with Turner's syndrome.
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http://dx.doi.org/10.1016/j.ajoc.2020.100662DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7132148PMC
June 2020

Large genetic diversity of Arcobacter butzleri isolated from raw milk in Southern Italy.

Food Microbiol 2020 Aug 28;89:103403. Epub 2019 Dec 28.

Experimental Zooprophylactic Institute of Apulia and Basilicata, Foggia, Via Manfredonia 20, 71122, Foggia, Italy.

Arcobacter butzleri is a zoonotic foodborne pathogen able to cause enteric and extraintestinal diseases. Its occurrence in foodstuff is well recognized worldwide but data on its presence in foods from Southern Italy are scarce. In this study the results on the occurrence and genotyping of Arcobacter spp. in bulk milk samples collected in Southern Italy are reported. Out of 484 samples, 64 (13.2%) resulted positive for the presence of Arcobacter spp. Using Real Time PCR but as few as 31.2% of these samples turned out as positive by using the cultural method, showing an overall prevalence of 4.1%. All isolates were identified as A. cryaerophilus using the biochemical identification whilst the sequencing of the atpA gene revealed that all the isolates were A. butzleri. Among the confirmed isolates, 16 different Sequence Types (ST) were identified using the Multi Locus Sequence Typing (MLST), 14 (87.5%) of which were previously unreported. Our survey reveals the presence of A. butzleri in bulk tank milk from Southern Italy and highlights the discrepancy between the two approaches used both for the detection (i.e., real time PCR vs cultural method) and the identification (i.e., biochemical test vs aptA sequencing) of Arcobacter spp In addition, a large genetic diversity among the isolates was detected and this makes the identification of source of the infections very challenging in outbreaks investigation.
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http://dx.doi.org/10.1016/j.fm.2019.103403DOI Listing
August 2020

Retinal vasproliferative tumor in a case of X-linked retinoschisis detachment.

Am J Ophthalmol Case Rep 2018 Mar 4;9:48-50. Epub 2018 Jan 4.

Department of Ophthalmology, Bascom Palmer Eye Institute, 900 NW17th St., Miami, FL 33136, USA.

Purpose: To describe the first published case of X-linked retinoschisis (XLRS) detachment with retinal vasoproliferative tumor (RVPT) and provide a literature review of the subject.

Observations: The authors describe a case of a 17 year old male with X-linked retinoschisis who presented with a retinal detachment and a retinal vasoproliferative tumor. The patient was treated with pars plana vitrectomy, endolaser, subtenon's kenalog and anti-VEGF (vascular endothelial growth factor) intravitreal injections. He regained 20/60 vision with a flat macula and had significant resolution of the associated vasoproliferative leakage seen on fluorescein angiography.

Conclusions And Importance: This case adds XLRS to the conditions associated with RVPT and gives support for treatment with laser photocoagulation and anti-VEGF therapy with bevacizumab to control the exudative process.
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http://dx.doi.org/10.1016/j.ajoc.2018.01.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5786862PMC
March 2018

Reproductive Parameters of Female Long- finned Pilot Whales () from the Southwestern Atlantic.

Zool Stud 2017 21;56:e39. Epub 2017 Dec 21.

Laboratorio de Mamíferos Marinos, Centro para el Estudio de Sistemas Marinos (CESIMAR). CENPAT-CONICET. E-mail:

Mass strandings represent a valuable source of information, especially when it comes to difficult-to-access pelagic species, such as Globicephala melas edwardii. We collected reproductive organs from 27 female pilot whales stranded in Caleta Malaspina, Chubut, Argentina. Our main objective was to analyze reproductive parameters of female long- finned pilot whales in the southern hemisphere. To achieve this, we studied age and reproductive status in order to approximate age and body length at sexual maturity, breeding interval, and fertility of this subspecies. The maturity state was determined by recording different ovulation bodies obtained from histological sections and by assessing the presence of milk in the mammary glands and a fetus, and we concluded that 5 of the females were immature and 22 were mature females. Finding mature females in different reproductive states (resting, in lactation, pregnant, or pregnant and in lactation) along with the great variety of fetus sizes would indicate that copulation and birth are not governed by seasonality. Age at sexual maturity was 8 years old and length at sexual maturity would reach 365.50 cm. The annual pregnancy rate was constant with age for mature females at 0.41 ovul / year, with a breeding interval of 2.4 years. The average fertility varied between 0.19 and 0.21. All the parameters are within the estimated range for females of the North Atlantic subpopulation, except for the breeding interval, which was significantly lower than estimates for the northern subspecies. This study could be limited because all individuals come from a single mass stranding. While this study allowed us to reach some conclusions about the reproductive biology of female in the Southwestern Atlantic Ocean, further studies are needed to fully comprehend the behavior and biology of this species.
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http://dx.doi.org/10.6620/ZS.2017.56-39DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6517729PMC
December 2017

Biventricular thrombosis in biventricular stress(takotsubo)-cardiomyopathy.

J Thromb Thrombolysis 2017 Aug;44(2):234-237

Cardiology Departement, Ospedale San Paolo, Bari, Italy.

Endo-ventricular thrombosis represents a possible clinical complication of stress(takotsubo)-cardiomyopathy (SC). Depressed ventricular systolic ventricular function, localized left ventricular (LV) dyskinesis, but also an increased pro-thrombotic state induced by catecholamine surge may facilitate the occurrence of endovascular thrombosis in SC. SC, however, may also present as right ventricular (RV) dysfunction or even as biventricular ballooning. Ventricular thrombosis may therefore theoretically occur in either ventricles or both. We report the case of an 88-year old woman, with vascular dementia and depression, admitted for abdominal pain, diarrhea, and rectal bleeding. Unexpectedly, electrocardiogram showed induced QT-prolongation with diffuse negative T-waves, while echocardiogram severe LV dysfunction (ejection fraction 35%), but also RV dysfunction and biventricular thrombosis. The diagnosis was therefore biventricular SC complicated by biventricular thrombosis; LV recovered after 10 days. When SC presents with a biventricular involvement, a careful assessment of either ventricular cavities should be therefore recommended to exclude the presence of (bi)ventricular thrombosis. It remains unresolved whether biventricular SC may represent a condition at higher risk of ventricular thrombosis.
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http://dx.doi.org/10.1007/s11239-017-1510-4DOI Listing
August 2017

Lipomatous Atrial Septal Hypertrophy: A Review of Its Anatomy, Pathophysiology, Multimodality Imaging, and Relevance to Percutaneous Interventions.

J Am Soc Echocardiogr 2016 08 7;29(8):717-723. Epub 2016 Jun 7.

Leon H. Charney Division of Cardiology, New York University Langone Medical Center, New York, New York. Electronic address:

Lipomatous atrial septal hypertrophy (LASH) is a histologically benign cardiac lesion characterized by excessive fat deposition in the region of the interatrial septum that spares the fossa ovalis. The etiology of LASH remains unclear, though it may be associated with advanced age and obesity. Because of the sparing of the fossa ovalis, LASH has a pathognomonic dumbbell shape. LASH may be mistaken for various tumors of the interatrial septum. Histologically, LASH is composed of both mature and brown (fetal) adipose tissue, but the role of brown adipose tissue remains unclear. In interventional procedures requiring access to the left atrium, LASH may interfere with transseptal puncture, as traversing the thickened area can reduce the maneuverability of catheters and devices. This may cause the needle to enter the epicardial space, causing dangerous pericardial effusions. LASH was once considered a contraindication to percutaneous device closure of atrial septal defects because of an associated increased risk for incorrect device deployment. However, careful attention to preprocedural imaging and procedural intracardiac echocardiography enable interventional cardiologists to perform procedures in patients with LASH without serious complications. In this review article, the authors describe anatomic and functional aspects of LASH, with emphasis on their roles in percutaneous interventions.
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http://dx.doi.org/10.1016/j.echo.2016.04.014DOI Listing
August 2016

Anti-Mullerian hormone may be a useful adjunct in the diagnosis of polycystic ovary syndrome in nonobese adolescents.

J Pediatr Endocrinol Metab 2014 Nov;27(11-12):1175-9

Objectives: This study aimed to [1] confirm that nonobese adolescents with polycystic ovary syndrome (PCOS) have higher anti-Mullerian hormone (AMH) than controls; [2] examine the relationship of AMH with PCOS features and hormonal profile; and [3] approximate an AMH value that discriminates between adolescents with PCOS and controls.

Design: Case-control study.

Setting: Subspecialty ambulatory clinic.

Patients: Thirty-one nonobese adolescent girls (age 13-21 years), 15 with PCOS diagnosed using the National Institutes of Health (NIH) criteria and 16 healthy control subjects. Subjects and controls were comparable for body mass index z-score, age and ethnicity.

Main Outcome Measure(s): AMH in PCOS subjects and control groups, correlation of AMH with hormonal parameters.

Results: AMH was higher in PCOS subjects (4.4±3.4 ng/mL) than in controls (2.4±1.3 ng/mL), when adjusted for menstrual age. In the entire group (PCOS and controls), AMH correlated with androgens, ovarian size and the presence of polycystic ovary (PCO) appearance. There was no difference in average ovarian size between PCOS (7.1±2.6 cm³) and controls (6.7±1.8 cm³). PCOS subjects were 1.49 times more likely to have AMH >3.4 ng/mL (confidence interval 0.98-2.26 ng/mL).

Conclusions: Our data suggest that AMH may be a useful adjunct in the diagnosis of PCOS in adolescents.
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http://dx.doi.org/10.1515/jpem-2014-0128DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4415850PMC
November 2014

The role of multimodality imaging in percutaneous left atrial appendage suture ligation with the LARIAT device.

J Am Soc Echocardiogr 2014 Jul 27;27(7):699-708. Epub 2014 May 27.

Leon H. Charney Division of Cardiology, New York University Langone Medical Center, New York, New York. Electronic address:

Atrial fibrillation (AF), the most common cardiac arrhythmia, is a significant cause of embolic stroke. Although systemic anticoagulation is the primary strategy for preventing the thromboembolic complications of AF, anticoagulants carry major bleeding risks, and many patients have contraindications to their use. Because thromboembolism typically arises from a clot in the left atrial appendage (LAA), local therapeutic alternatives to systemic anticoagulation involving surgical or percutaneous exclusion of the LAA have been developed. Surgical exclusion of the LAA is typically performed only as an adjunct to other cardiac surgeries, thus limiting the number of eligible patients. Furthermore, surgical exclusion of the LAA is frequently incomplete, and thromboembolism may still occur. Percutaneous LAA exclusion includes two approaches: transseptal delivery of an occlusion device to the LAA and epicardial suture ligation of the LAA, the LARIAT procedure. In the LARIAT procedure, a pretied snare is placed around the epicardial surface of the LAA orifice via pericardial access. Proper snare placement is achieved with epicardial and endocardial magnet-tipped guidewires. The endocardial wire is advanced transvenously to the LAA apex after transseptal puncture. The epicardial wire, introduced into the pericardial space, achieves end-to-end union with the endocardial wire at the LAA apex. The snare is then placed over the LAA, tightened, and sutured. On the basis of early clinical experience, the LARIAT procedure has a high success rate of LAA exclusion with low risk for complications. The authors describe the indispensable role of real-time transesophageal echocardiography in the guidance of LAA epicardial suture ligation with the LARIAT device.
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http://dx.doi.org/10.1016/j.echo.2014.04.014DOI Listing
July 2014

Determination of physicochemical properties of delipidized hair.

J Cosmet Sci 2013 Sep-Oct;64(5):355-70

Materials Science Department, Corporate R&D, Ashland Specialty Ingredients, Wayne, NJ 07470.

Using various physicochemical methods of analysis, we examined human hair in its virgin and delipidized state. Free lipids were removed by a solvent extraction technique (covalently bound lipids were not removed) using a series of solvents with varying polarity. We analyzed the surface properties of hair by conducting mechanical combing and dynamic contact angle analysis. In addition, we used inverse gas chromatography surface energy analysis to explore the chemical composition of the hair surface based on interactions of various nonpolar and polar probes with biological molecules residing on the hair surface. Further, we investigated the importance that free lipids play in the internal structural properties of hair using dynamic scanning calorimetry and tensile strength measurements. The microstructure of the hair surface was probed by atomic force microscopy, whereas the lipid content of hair's morphological components was determined by infrared spectroscopic imaging. We also monitored the water management properties of virgin and delipidized hair by dynamic vapor sorption, which yielded unique water sorption isotherms for each hair type. Using all these techniques, differences were found in the chemical composition and physical behavior of virgin and delipidized hair. To better understand the influence of hair lipid composition on hair styling treatments, we conducted mechanical analyses of hair shaped into omega loops to determine the stiffness, elasticity, and flexibility of hair-polymer assemblies. Although there were no discernible differences between untreated virgin and delipidized hair, in terms of stiffness and elasticity, we found that treatment with hair styling agents produced different effects depending on the hair type used. Likewise, streaming potential measurements were carried out to monitor the binding capacity of rinse-off treatments on virgin and delipidized hair. Using this technique, we monitored the surface potential of hair and found significant differences in the binding behavior of cationic polymers and surfactants (polyquaternium-55 and quaternium-26) on both hair types.
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December 2013

Influence of various environmental parameters on sweat gland activity.

J Cosmet Sci 2013 Jul-Aug;64(4):243-60

Department of Materials Science, Corporate R&D, Ashland Specialty Ingredients, Wayne, NJ, USA.

The choice of environmental conditions when conducting antiperspirant studies greatly affects the quantity of sweat output. Our initial goal in this work was to develop an in-house procedure to test the efficacy of antiperspirant products using replica techniques in combination with image analysis. To ameliorate the skin replica method, we conducted rheological studies using dynamic mechanical analysis of the replica formulation. In terms of sweat output quantification, our preliminary results revealed a considerable amount of variation using the replica technique, leading us to conduct more fundamental studies of the factors that influence sweating behavior and how to best design the experimental strategy. In accordance with the FDA's protocol for antiperspirant testing, we carried out gravimetric analyses of axillae sweating under a variety of environmental conditions including temperature and humidity control. Subjects were first acclimatized in an environmentally controlled room for 30 min, and then placed in a sauna for an additional 30 or 45 min, depending on which test we administered. In Test 1 (30 min total in the sauna), the first 10 min in the sauna was another equilibration period, followed by a 20 min sweat production stage. We monitored axillae sweating during the last 20 min in the sauna by gravimetric analysis. At time (t) = 30 min in the sauna, skin replicas were taken and later analyzed using imaging and image analysis techniques. Test 1 was carried out on over 25 subjects, both male and female, from various racial backgrounds. In Test 2, subjects spent 45 min in the sauna after the initial 30-min period in the environmental room. During the 45 min, we obtained gravimetric readings of absorbent pads placed in the axillae. We conducted studies at various temperature and relative humidity settings. We also studied the influence of several external parameters on sudoriferous activity. Test 2 was a range-finding experiment on two subjects to determine the optimized environmental conditions for the hot room procedure. In addition to the replica and gravimetric techniques, we also measured flux density to determine the onset of firing of sweat glands to ensure that our environmental preconditioning step (30 min in the environmental room) brought subjects to the point that their sweat glands were activated. Although flux density measurements are usually carried out to determine transepidermal water loss (TEWL), we found that they can be equally useful for monitoring the onset of sweat production. Thermal infrared imaging experiments were also carried out allowing us to generate full-body images of subjects containing anatomical thermal distribution data with high accuracy. Overall, we conclude that our in-house hot room procedure offers much potential as an effective and cost-efficient screening tool for narrowing copious antiperspirant formulations to a select few for expensive clinical evaluation.
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September 2013

Infrared spectroscopy and differential scanning calorimetry studies of binary combinations of cis-6-octadecenoic acid and octadecanoic acid.

Chem Phys Lipids 2007 Nov 26;150(1):109-15. Epub 2007 Jun 26.

ISP, 1361 Alps Road, Wayne, NJ 07470, USA.

Fourier transform infrared (FTIR) spectroscopy and differential scanning calorimetry (DSC) studies are reported for combinations of cis-6-octadecenoic acid (also termed petroselinic acid, PSA) and octadecanoic acid (also termed stearic acid, SA) across a wide range of binary mole ratio combinations. The data are then used to plot the phase diagram which is found to be montotectic with the PSA reducing the melting temperature of SA at all compositions. The relevance of these experiments to stratum corneum (SC) biophysical behavior, particularly the influence and potential mechanisms of PSA on dermal permeation, is discussed. The potential role of cis-6-octadecenoic acid as a permeation enhancer is discussed in the context of these studies of its interaction with saturated fatty acids.
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http://dx.doi.org/10.1016/j.chemphyslip.2007.06.217DOI Listing
November 2007

Safety, efficacy, and immunogenicity of a live, quadrivalent human-bovine reassortant rotavirus vaccine in healthy infants.

J Pediatr 2004 Feb;144(2):184-90

University of Pennsylvania School of Medicine, Philadelphia, and Merck & Co, Inc, West Point, Pennsylvania 19486, USA.

Objectives: To investigate safety, efficacy, and immunogenicity of live quadrivalent rotavirus vaccine (QRV) containing human-bovine (WC3) reassortant rotavirus serotypes G1, G2, G3, and P1a.

Study Design: This was a randomized, double-blinded, placebo-controlled trial. During 1993 to 1994, at 10 US study sites, 439 healthy infants approximately 2 to 6 months of age, were enrolled to receive 3 doses of oral QRV or placebo at approximately 8-week intervals.

Results: The vaccine was generally well tolerated; no serious vaccine-related adverse experiences were reported. Risk differences and 95% confidence intervals suggested no differences between vaccine and placebo recipients in the incidences of fever, irritability, vomiting, or diarrhea during the 14 days after any dose. QRV was 74.6% efficacious (95% CI: 49.5%, 88.3%) in preventing rotavirus acute gastroenteritis (AGE), regardless of severity and 100% efficacious (95% CI: 43.5%, 100%) in preventing severe rotavirus AGE through one rotavirus season. Serotype G1 was identified in most infants with rotavirus AGE. A >or=3-fold rise in serum neutralizing antibody to G1 was observed in 57% (45/79) of vaccinees. A >or=3-fold rise in serum anti-rotavirus IgA and fecal anti-rotavirus IgA was observed in 88% (162/185) and 65% (104/159) of vaccinees, respectively.

Conclusions: QRV was generally well tolerated, immungenic, and highly effective against rotavirus gastroenteritis.
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http://dx.doi.org/10.1016/j.jpeds.2003.10.054DOI Listing
February 2004

Influence of hydrophilic and lipophilic beta-blockers on heart rate, ventricular repolarization and their interrelationship in normal subjects.

Ital Heart J 2000 May;1(5):331-5

Institute of Cardiology, University of Bari, Italy.

Background: It has been hypothesized that hydrophilic and lipophilic beta-blockers have different antiarrhythmic properties because only the latter seem to reduce the rate of sudden death in post-myocardial infarction patients as well as animal models which seem to be independent of their effect on autonomic nervous system modulation. The aim of this study was to evaluate the different effects of a hydrophilic (nadolol) and lipophilic (metoprolol) beta-blocker on ventricular repolarization in normal subjects.

Methods: Seventeen normal subjects entered this randomized, single-blind cross-over study designed to compare the effects of nadolol (80 mg/day) and slow-release metoprolol (200 mg/day) on dynamic ventricular repolarization. The RR intervals, the QT evaluated at the apex (QT apex) and at the end (QT end) of the T wave before and after correction for heart rate, the standard deviation of QT apex and QT end, and the slope of the QT/RR linear relationship (QTa-slope and QTe-slope) were studied using the ELATEC system (ELA Medical, Mountrouge, France), and an evaluation was made of their reproducibility and the effects of each beta-blocker.

Results: The most reproducible parameters were QT apex, corrected QT apex and the QTe-slope. Nadolol was associated with a greater adrenergic blockade than metoprolol (lengthening of RR interval +25 +/- 7 and +17 +/- 8% respectively, p = 0.0003) and a lower effect on ventricular repolarization (reduction of corrected QT apex -0.6 +/- 3 and -2.5 +/- 2.1% respectively, p < 0.01; reduction of QTe-slope -5 +/- 16 and -15 +/- 15% respectively, p = 0.03).

Conclusions: At the dosages used in the study, metoprolol showed lower adrenergic blockade but greater effect on ventricular repolarization than nadolol.
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May 2000

Effects of atrazine on Ochrobactrum anthropi membrane fatty acids.

Appl Environ Microbiol 1996 Jul;62(7):2644-6

Istituto di Ricerche Farmacologiche Mario Negri, Consorzio Mario Negri Sud, "Gennaro Paone" Environmental Health Center, Santa Maria Imbaro, Italy.

Ochrobactrum anthropi is a gram-negative bacillus recognized as a human opportunist pathogen isolated in clinical specimens and not of clinical significance. We report a new aspect of this bacterium, that it has been isolated from activated sludge. In fact, it is able to grow on atrazine (2-chloro-4-ethylamino-6-isopropyl-amine-s-triazine) by utilizing it as the only source of carbon. Our results show that atrazine (0.03 g/liter) causes a dramatical increase in the degree of saturation of membrane fatty acids. Analysis and identification of bacterial fatty acids were performed by gas chromatography and gas chromatography-mass spectrometry techniques.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC168045PMC
http://dx.doi.org/10.1128/AEM.62.7.2644-2646.1996DOI Listing
July 1996