Publications by authors named "Lanjun Liu"

2 Publications

  • Page 1 of 1

Efficient mutagenesis targeting the gene in mice using a combination of Cas9 protein and dual gRNAs.

Am J Transl Res 2021 15;13(10):12094-12106. Epub 2021 Oct 15.

Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University Nanjing 210046, Jiangsu, China.

We injected mouse zygotes with combinations of Cas9 protein, mRNA, and two gRNAs targeting a single exon of type I interferon receptor () to determine the gene targeting efficiencies. Cas9 protein produced on-target mutations more efficiently than mRNA when each was used with a single gRNA, regardless of which gRNA was used. When mRNA and Cas9 protein were co-injected, the on-target efficiency could reach 97.0% when both gRNAs were used, which was higher than when either gRNA was used alone (61.3% and 75.5%, respectively; P<0.05). Co-injection of Cas9 protein with both gRNAs produced the highest on-target mutation rate of any combination (100.0%). Most on-target mutations were deletions of 2 to 113 nucleotides, and there were few off-target mutations in mutant animals. The expression intensity of IFNAR1 was reduced in heterozygous mice (IF) and almost or completely absent in homozygous null mice compared with that in wild-type mice (IF and Western blot). When both gRNAs targeting were used simultaneously with two gRNAs targeting , the on-target editing efficiency on each gene was 96.8% and 85.5%, respectively. Co-injection of dual gRNAs and Cas9 protein is an efficient approach for knockout and multi-gene editing in mice and may be applied in other animal models and breeding livestock.
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October 2021

A Seabed Real-Time Sensing System for In-Situ Long-Term Multi-Parameter Observation Applications.

Sensors (Basel) 2019 Mar 12;19(5). Epub 2019 Mar 12.

Key Laboratory of Marine Environment and Geological Engineering Shandong Province, Ocean University of China, Qingdao 266100, China.

Aiming at the real-time observation requirements in marine science and ocean engineering, based on underwater acoustic communication and satellite communication technology, a seabed real-time sensing system for in-situ long-term multi-parameter observation applications (SRSS/ILMO) is proposed. It consists of a seabed observation system, a sea surface relay transmission buoy, and a remote monitoring system. The system communication link is implemented by underwater acoustic communication and satellite communication. The seabed observation system adopts the "ARM + FPGA" architecture to meet the low power consumption, scalability, and versatility design requirements. As a long-term unattended system, a two-stage anti-crash mechanism, an automatic system fault isolation design, dual-medium data storage, and improved Modbus protocol are adopted to meet the system reliability requirements. Through the remote monitoring system, users can configure the system working mode, sensor parameters and acquire observation data on demand. The seabed observation system can realize the observation of different fields by carrying different sensors such as those based on marine engineering geology, chemistry, biology, and environment. Carrying resistivity and pore pressure sensors, the SRSS/ILMO powered by seawater batteries was used for a seabed engineering geology observation. The preliminary test results based on harbor environment show the effectiveness of the developed system.
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March 2019