Publications by authors named "Lang Wang"

93 Publications

MircroRNA Profiles of Early Rice Inflorescence Revealed a Specific miRNA5506 Regulating Development of Floral Organs and Female Megagametophyte in Rice.

Int J Mol Sci 2021 Jun 21;22(12). Epub 2021 Jun 21.

Center of Experimental Teaching for Common Basic Courses, South China Agricultural University, Guangzhou 510642, China.

The developmental process of inflorescence and gametophytes is vital for sexual reproduction in rice. Multiple genes and conserved miRNAs have been characterized to regulate the process. The changes of miRNAs expression during the early development of rice inflorescence remain unknown. In this study, the analysis of miRNAs profiles in the early stage of rice inflorescence development identified 671 miRNAs, including 67 known and 44 novel differentially expressed miRNAs (DEMs). Six distinct clusters of miRNAs expression patterns were detected, and Cluster 5 comprised 110 DEMs, including unconserved, rice-specific osa-miR5506. Overexpression of osa-miR5506 caused pleiotropic abnormalities, including over- or under-developed palea, various numbers of floral organs and spikelet indeterminacy. In addition, the defects of ovaries development were frequently characterized by multiple megasporocytes, ovule-free ovary, megasporocyte degenerated and embryo sac degenerated in the transgenic lines. osa-miR5506 targeted REM transcription factor . Summarily, these results demonstrated that rice-specific osa-miR5506 plays an essential role in the regulation of floral organ number, spikelet determinacy and female gametophyte development in rice.
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http://dx.doi.org/10.3390/ijms22126610DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8235126PMC
June 2021

CRISPR-Cas13a-Based Detection for Bovine Viral Diarrhea Virus.

Front Vet Sci 2021 9;8:603919. Epub 2021 Jun 9.

College of Life Sciences, Shihezi University, Shihezi, China.

Bovine Viral Diarrhea Virus (BVDV) is the main pathogen of bovine viral diarrhea disease (BVD), which leads to enormous economic losses in the cattle industry. A sensitive and specific detection for BVDV is advantageous to the control of BVDV. Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas systems have been used for detecting virus RNA. In this study, the expression and purification of LwCas13a protein was optimized and the RNase activity of LwCas13a was verified. CRISPR-LwCas13a system could detect BVDV virus and BVDV RNA with high specificity and simplicity. The detection limit of the LwCas13a system was 10 pM, and there were no cross-reactions with HEK293T and MDBK. In summary, a sensitive, specific, and simple nucleic acid detection method based on CRISPR-Cas13a was developed for BVDV. This method provides a new detection strategy for early diagnosis of BVDV.
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http://dx.doi.org/10.3389/fvets.2021.603919DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8219879PMC
June 2021

Microglia, synaptic dynamics and forgetting.

Brain Res Bull 2021 Jun 12;174:173-183. Epub 2021 Jun 12.

Center of Stem Cell and Regenerative Medicine, Department of Neurology of the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310058, China. Electronic address:

Microglia are the major immune cells in the brain parenchyma. Besides their immune functions, microglia are important in regulating the dynamics of synapses. It is believed that the stability of synapses is essential for long-term storage and retrieval of memories, whereas microglial regulation of synaptic dynamics could affect the stability of memories, thus providing a potential mechanism for forgetting. In this review, we focus on the regulation of synaptic dynamics by microglia, as well as the subsequent effects on memory and forgetting, under physiological and pathological conditions. Revealing microglial regulation of synaptic dynamics will not only illuminate the physiological functions of microglia in the brain, but also provide us a new perspective to study the molecular and cellular mechanisms underlying forgetting. In addition, this will also improve our understanding of the process of memory encoding, storage and retrieval in the brain. Furthermore, uncovering the mechanisms through which microglia act on synaptic dynamics in pathological conditions will provide new strategies for the prevention and treatment of memory impairment in diseases.
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http://dx.doi.org/10.1016/j.brainresbull.2021.06.005DOI Listing
June 2021

High-dose vitamin C intravenous infusion in the treatment of patients with COVID-19: A protocol for systematic review and meta-analysis.

Medicine (Baltimore) 2021 May;100(19):e25876

Integrated TCM & Western Medicine Department, Zhuzhou Central Hospital, Zhuzhou, Hunan province, China.

Background: Patients infected with a virus usually lack vitamin C. High-dose vitamin C has an antiviral effect, and has been used by several researchers to treat COVID-19 by intravenous infusion, achieving good results. However, the efficacy and safety of vitamin C in the treatment of patients with COVID-19 remain unclear. Thus, the aim of the present study was to investigate the efficacy of high-dose vitamin C infusion in the treatment of patients with COVID-19.

Methods: Electronic databases were searched, including PubMed, EMBASE, Cochrane Central Register of Controlled Trials, Web of Science, China National Knowledge Infrastructure database, Chinese Wanfang database, and Chinese Biomedical Literature database. The aim was to collect randomized controlled trials of high-dose vitamin C infusion in the treatment of patients with COVID-19, with the retrieval time being from the establishment of the database to March 2021. In accordance with the pre-designed inclusion/exclusion criteria, all data were extracted independently by 2 researchers. To assess the risk bias in the studies, the Cochrane collaboration's tool for assessing risk of bias was used to assess the risk bias in the studies, while meta-analysis was performed using Revman 5.3 software.

Results: In the present study, a high-quality comprehensive evaluation is provided of high-dose vitamin C infusion in the treatment of patients with COVID-19.

Conclusion: Further convincing evidence for the clinical treatment of COVID-19 is provided, in addition to evidence-based guidance for clinical practice.

Prospero Registration Number: CRD42021246342.
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http://dx.doi.org/10.1097/MD.0000000000025876DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8133047PMC
May 2021

Editorial: Mechanisms Underlying Experience-Dependent Plasticity of Cortical Circuits.

Front Cell Neurosci 2021 30;15:687297. Epub 2021 Apr 30.

Skirball Institute, Neuroscience Institute, Department of Otolaryngology, New York University Grossmna School of Medicine, New York, NY, United States.

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http://dx.doi.org/10.3389/fncel.2021.687297DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8121167PMC
April 2021

Risk factors for post-traumatic stress disorder in acute trauma patients: A protocol for systematic review and meta-analysis.

Medicine (Baltimore) 2021 Apr;100(17):e25616

Department of Metabolic Endocrinology, Zhuzhou Central Hospital, Zhuzhou, Hunan province, China.

Background: Post-traumatic stress disorder (PTSD) is one of the most commonly reported mental health consequences, followed by disasters and traumatic events, either natural or man-made. At present, there are no unified results for the prevalence rate of PTSD in patients suffering from acute trauma and related influencing factors. Therefore, the purpose of this study is to systematically evaluate the existing literatures, thus obtaining a comprehensive estimation of the combined prevalence rate of PTSD and related factors in trauma patients, so as to provide evidence support for clinical disease prediction models and intervention strategies.

Methods: Published articles will be retrieved from PubMed, Embase, Cochrane Library, Web of Science, China Biology Medicine Database, China National Knowledge Infrastructure, China Science and Technology Journal Database, and Wanfang Database. Research reports will be searched in March 2021. STATA 14.0 software will be applied for data analysis. Mantel-Haenszel fixed effect model or DerSimonian-Laird random effect model will be selected to estimate the pooled prevalence of PTSD in patients with acute trauma and associated factors.

Results: We will disseminate the findings of this systematic review and meta-analysis via publications in peer-reviewed journals.

Conclusions: The results of this analysis can be used to establish a risk prediction model of PTSD in patients experiencing acute trauma, so as to provide intervention strategies.

Osf Registration Number: DOI 10.17605/OSF.IO/Z275U.
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http://dx.doi.org/10.1097/MD.0000000000025616DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8084095PMC
April 2021

Effects of carotid baroreceptor stimulation on aortic remodeling in obese rats.

Nutr Metab Cardiovasc Dis 2021 05 9;31(5):1635-1644. Epub 2021 Feb 9.

Department of Cardiology, Renmin Hospital of Wuhan University, 238 Jiefang Road, Wuhan 430060, People's Republic of China; Cardiovascular Research Institute, Wuhan University, Wuhan 430060, People's Republic of China; Hubei Key Laboratory of Cardiology, Wuhan 430060, People's Republic of China. Electronic address:

Background And Aim: Our previous study found carotid baroreceptor stimulation (CBS) reduces body weight and white adipose tissue (WAT) weight, restores abnormal secretion of adipocytokines and inflammation factors, decreases systolic blood pressure (SBP) by inhibiting activation of sympathetic nervous system (SNS) and renin-angiotensin system (RAS) in obese rats. In this study, we explore effects of CBS on aortic remodeling in obese rats.

Methods And Results: Rats were fed high-fat diet (HFD) for 16 weeks to induce obesity and underwent either CBS device implantation and stimulation or sham operation at 8 weeks. BP and body weight were measured weekly. RAS activity of WAT, histological, biochemical and functional profiles of aortas were detected after 16 weeks. CBS effectively decreased BP in obese rats, downregulated mRNA expression of angiotensinogen (AGT) and renin in WAT, concentrations of AGT, renin, angiotensin II (Ang II), protein levels of Ang II receptor 1 (AT1R) and Ang II receptor 2 (AT2R) in WAT were declined. CBS inhibited reactive oxygen species (ROS) generation, inflammatory response and endoplasmic reticulum (ER) stress in aortas of obese rats, restrained vascular wall thickening and vascular smooth muscle cells (VSMCs) phenotypic switching, increased nitric oxide (NO) synthesis, promoted endothelium-dependent vasodilatation by decreasing protein expression of AT1R and leptin receptor (LepR), increasing protein expression of adiponectin receptor 1 (AdipoR1) in aortic VSMCs.

Conclusion: CBS reduced BP and reversed aortic remodeling in obese rats, the underlying mechanism might be related to the suppressed SNS activity, restored adipocytokine secretion and restrained RAS activity of WAT.
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http://dx.doi.org/10.1016/j.numecd.2021.01.021DOI Listing
May 2021

MicroRNA-665 Regulates Cell Proliferation and Apoptosis of Vascular Smooth Muscle Cells by Targeting TGFBR1.

Int Heart J 2021 Mar 17;62(2):371-380. Epub 2021 Mar 17.

Department of Cardiology, Luotian Wanmizhai Hospital.

Coronary artery disease (CAD) is one of the heavy health burdens worldwide. Aberrant proliferation of vascular smooth muscle cells (VSMCs) contributes to the occurrence and development of CAD. This study aimed at exploring differentially expressed microRNAs (miRNAs) and their regulatory mechanisms in the development of CAD.The miRNA expression profile of GSE28858 was obtained from the Gene Expression Omnibus database. Differentially expressed miRNAs (DEmiRNAs) between CAD and healthy control samples were analyzed using limma package in R. Target genes of DEmiRNAs were predicted, and a miRNA-target gene network was constructed. The relationship between miR-665 and transforming growth factor beta receptor 1 (TGFBR1) was selected for further analysis. The interaction between miR-665 and TGFBR1 was confirmed by dual luciferase reporter assay. Effects of miR-665 on cell viability and apoptosis of VSMCs were evaluated by cell counting kit-8 (CCK-8) assay and flow cytometry, respectively. Besides, western blot assays for BCL2L11 and caspase 3 were also conducted.A total of 38 upregulated miRNAs and 28 downregulated miRNAs were identified. The expression level of miR-665 was significantly downregulated in patients with CAD. TGFBR1 was proved to be a target gene of miR-665. Besides, ectopic expression of miR-665 obviously inhibited VSMC growth and promoted VSMC apoptosis. TGFBR1 overexpression in VSMCs transfected with miR-665 mimic could restore the effect of miR-665 on the proliferation and apoptosis of VSMCs.MiR-665 might participate in the proliferation and apoptosis of VSMCs by targeting TGFBR1.
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http://dx.doi.org/10.1536/ihj.20-016DOI Listing
March 2021

Oral high dose vitamin D for the treatment of diabetic patients with COVID-19: A protocol for systematic review and meta-analysis.

Medicine (Baltimore) 2021 Mar;100(9):e24517

Department of metabolic Endocrinology, Zhuzhou Central Hospital, Zhuzhou, Hunan, China.

Background: Type 2 diabetes mellitus patients complicated with infections experience severe vitamin D deficiency. High-dose vitamin D is applied to the treatment of corona virus disease 2019 (COVID-19) by some researchers, and good results have been achieved. However, the efficacy of vitamin D in the treatment of infections in COVID-19 patients with diabetes remains unclarified. This study aims to explore the effect of oral high-dose vitamin D in the treatment of diabetic patients with COVID-19.

Methods: Randomized controlled trials about the application of high-dose vitamin D in the treatment of diabetic patients with COVID-19 will be retrieved from such electronic databases as Embase, PubMed, Cochrane Central Register of Controlled Trials, China National Knowledge Infrastructure database, Chinese Wanfang database and Chinese Biomedical Literature database. The retrieval time is from their inception to December 2020. According to the pre-designed inclusion/exclusion criteria, the data will be extracted independently by two researchers. The risk of bias of the included studies will be assessed by the Cochrane collaboration's tool. Meta-analysis will be conducted by using Revman 5.3 software.

Results: A high-quality and comprehensive evaluation of oral high-dose vitamin D for the treatment of diabetic patients with COVID-19 will be made.

Conclusion: The article will provide more convincing evidence and evidence-based guidance for clinical practice.

Ethics And Dissemination: The private information of individuals will not be made public, and this systematic evaluation will also not infringe on the rights of participants. Ethical approval is not required. Research results may be published in a peer-reviewed journal or disseminated in relevant conferences.

Prospero Registration Number: CRD42020214284.
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http://dx.doi.org/10.1097/MD.0000000000024517DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7939193PMC
March 2021

JKAMP inhibits the osteogenic capacity of adipose-derived stem cells in diabetic osteoporosis by modulating the Wnt signaling pathway through intragenic DNA methylation.

Stem Cell Res Ther 2021 02 12;12(1):120. Epub 2021 Feb 12.

Department of Oral and Maxillofacial Surgery, The Affiliated Stomatology Hospital of Southwest Medical University, Luzhou, 646000, China.

Background: Diabetic osteoporosis (DOP) is a systemic metabolic bone disease caused by diabetes mellitus (DM). Adipose-derived stem cells (ASCs) play an important role in bone regeneration. Our previous study confirmed that ASCs from DOP mice (DOP-ASCs) have a lower osteogenesis potential compared with control ASCs (CON-ASCs). However, the cause of this poor osteogenesis has not been elucidated. Therefore, this study investigated the underlying mechanism of the decline in the osteogenic potential of DOP-ASCs from the perspective of epigenetics and explored methods to enhance their osteogenic capacity.

Methods: The expression level of JNK1-associated membrane protein (JKAMP) and degree of DNA methylation in CON-ASCs and DOP-ASCs were measured by mRNA expression profiling and MeDIP sequencing, respectively. JKAMP small interfering RNA (siRNA) and a Jkamp overexpression plasmid were used to assess the role of JKAMP in osteogenic differentiation of CON-ASCs and DOP-ASCs. Immunofluorescence, qPCR, and western blotting were used to measure changes in expression of Wnt signaling pathway-related genes and osteogenesis-related molecules after osteogenesis induction. Alizarin red and ALP staining was used to confirm the osteogenic potential of stem cells. Bisulfite-specific PCR (BSP) was used to detect JKAMP methylation degree.

Results: Expression of JKAMP and osteogenesis-related molecules (RUNX2 and OPN) in DOP-ASCs was decreased significantly in comparison with CON-ASCs. JKAMP silencing inhibited the Wnt signaling pathway and reduced the osteogenic ability of CON-ASCs. Overexpression of JKAMP in DOP-ASCs rescued the impaired osteogenic capacity caused by DOP. Moreover, JKAMP in DOP-ASCs contained intragenic DNA hypermethylated regions related to the downregulation of JKAMP expression.

Conclusions: Intragenic DNA methylation inhibits the osteogenic ability of DOP-ASCs by suppressing expression of JKAMP and the Wnt signaling pathway. This study shows an epigenetic explanation for the reduced osteogenic ability of DOP-ASCs and provides a potential therapeutic target to prevent and treat osteoporosis.
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http://dx.doi.org/10.1186/s13287-021-02163-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7881648PMC
February 2021

Premigratory neural crest stem cells generate enteric neurons populating the mouse colon and regulating peristalsis in tissue-engineered intestine.

Stem Cells Transl Med 2021 Jun 22;10(6):922-938. Epub 2021 Jan 22.

Institute of Translational Medicine, and Children's Hospital Affiliated and Key Laboratory of Diagnosis and Treatment of Neonatal Diseases of Zhejiang Province, the Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, Hangzhou, People's Republic of China.

Hirschsprung's disease (HSCR) is a common congenital defect. It occurs when bowel colonization by neural crest-derived enteric nervous system (ENS) precursors is incomplete during the first trimester of pregnancy. Several sources of candidate cells have been previously studied for their capacity to regenerate the ENS, including enteric neural crest stem cells (En-NCSCs) derived from native intestine or those simulated from human pluripotent stem cells (hPSCs). However, it is not yet known whether the native NCSCs other than En-NCSCs would have the potential of regenerating functional enteric neurons and producing neuron dependent motility under the intestinal environment. The present study was designed to determine whether premigratory NCSCs (pNCSCs), as a type of the nonenteric NCSCs, could form enteric neurons and mediate the motility. pNCSCs were firstly transplanted into the colon of adult mice, and were found to survive, migrate, differentiate into enteric neurons, and successfully integrate into the adult mouse colon. When the mixture of pNCSCs and human intestinal organoids was implanted into the subrenal capsule of nude mice and grown into the mature tissue-engineered intestine (TEI), the pNCSCs-derived neurons mediated neuron-dependent peristalsis of TEI. These results show that the pNCSCs that were previously assumed to not be induced by intestinal environment or cues can innervate the intestine and establish neuron-dependent motility. Future cell candidates for ENS regeneration may include nonenteric NCSCs.
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http://dx.doi.org/10.1002/sctm.20-0469DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8133337PMC
June 2021

Time-Restricted Salutary Effects of Blood Flow Restoration on Venous Thrombosis and Vein Wall Injury in Mouse and Human Subjects.

Circulation 2021 Mar 15;143(12):1224-1238. Epub 2021 Jan 15.

Cardiovascular Research Center, Cardiology Division, Massachusetts General Hospital, Harvard Medical School, Boston (W.L., C.W.K., M.O., L.W., A.T., F.A.J.).

Background: Up to 50% of patients with proximal deep vein thrombosis (DVT) will develop the postthrombotic syndrome characterized by limb swelling and discomfort, hyperpigmentation, skin ulcers, and impaired quality of life. Although catheter-based interventions enabling the restoration of blood flow (RBF) have demonstrated little benefit on postthrombotic syndrome, the impact on the acuity of the thrombus and mechanisms underlying this finding remain obscure. In experimental and clinical studies, we examined whether RBF has a restricted time window for improving DVT resolution.

Methods: First, experimental stasis DVT was generated in C57/BL6 mice (n=291) by inferior vena cava ligation. To promote RBF, mice underwent mechanical deligation with or without intravenous recombinant tissue plasminogen activator administered 2 days after deligation. RBF was assessed over time by ultrasonography and intravital microscopy. Resected thrombosed inferior vena cava specimens underwent thrombus and vein wall histological and gene expression assays. Next, in a clinical study, we conducted a post hoc analysis of the ATTRACT (Acute Venous Thrombosis: Thrombus Removal with Adjunctive Catheter-Directed Thrombolysis) pharmacomechanical catheter-directed thrombolysis (PCDT) trial (NCT00790335) to assess the effects of PCDT on Venous Insufficiency Epidemiological and Economic Study quality-of-life and Villalta scores for specific symptom-onset-to-randomization timeframes.

Results: Mice that developed RBF by day 4, but not later, exhibited reduced day 8 thrombus burden parameters and reduced day 8 vein wall fibrosis and inflammation, compared with controls. In mice without RBF, recombinant tissue plasminogen activator administered at day 4, but not later, reduced day 8 thrombus burden and vein wall fibrosis. It is notable that, in mice already exhibiting RBF by day 4, recombinant tissue plasminogen activator administration did not further reduce thrombus burden or vein wall fibrosis. In the ATTRACT trial, patients receiving PCDT in an intermediate symptom-onset-to-randomization timeframe of 4 to 8 days demonstrated maximal benefits in Venous Insufficiency Epidemiological and Economic Study quality-of-life and Villalta scores (between-group difference=8.41 and 1.68, respectively, <0.001 versus patients not receiving PCDT). PCDT did not improve postthrombotic syndrome scores for patients having a symptom-onset-to-randomization time of <4 days or >8 days.

Conclusions: Taken together, these data illustrate that, within a restricted therapeutic window, RBF improves DVT resolution, and PCDT may improve clinical outcomes. Further studies are warranted to examine the value of time-restricted RBF strategies to reduce postthrombotic syndrome in patients with DVT.
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http://dx.doi.org/10.1161/CIRCULATIONAHA.120.049096DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7988304PMC
March 2021

Association of Interleukin-6 Levels with Morbidity and Mortality in Patients with Coronavirus Disease 2019 (COVID-19).

Jpn J Infect Dis 2021 Jul 30;74(4):293-298. Epub 2020 Nov 30.

Department of Cardiology, Renmin Hospital of Wuhan University, People's Republic of China.

The prognostic value of interleukin-6 (IL-6) in coronavirus disease 2019 (COVID-19) needs to be clarified. In this retrospective study, COVID-19 patients treated at Renmin Hospital of Wuhan University from January 7 to February 8, 2020 with measurements of serum IL-6 levels within 1 week after admission were included. Data regarding demographics, clinical characteristics, laboratory tests, complications, and outcomes were collected and analyzed. Sixty-six patients diagnosed with COVID-19 were included in this study (31 patients were females). They were divided into a normal group (serum IL-6 <10 pg/mL, n = 35) and an abnormal group (serum IL-6 <10 pg/mL, n = 31). Compared with the normal group, the incidence of critical cases (P <0.001), acute respiratory distress syndrome (ARDS) (P = 0.001), acute cardiac injury (P = 0.002), cardiac insufficiency (P = 0.039), mechanical ventilation rate (P = 0.002), and mortality (P = 0.021) was significantly increased in the abnormal group. Serum IL-6 concentration was an independent predictor of fatal outcome (P = 0.04). The optimal cutoff value of serum IL-6 concentration for predicting fatal outcomes was 26.09 pg/mL (P <0.001). In COVID-19, elevated serum IL-6 levels were associated with critical illness, use of mechanical ventilation, and complications, including heart injury and ARDS, and could predict a fatal outcome. Early detection of serum IL-6 levels after admission should be necessary in COVID-19 patients.
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http://dx.doi.org/10.7883/yoken.JJID.2020.463DOI Listing
July 2021

Prolonged prothrombin time at admission predicts poor clinical outcome in COVID-19 patients.

World J Clin Cases 2020 Oct;8(19):4370-4379

Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China.

Background: The prognostic value of coagulation disorder in coronavirus disease 2019 (COVID-19) patients should be demonstrated.

Aim: To investigate the abnormalities of coagulation parameters in the patients with COVID-19 and their prognostic values.

Methods: Consecutive patients admitted in the isolation ward of Renmin Hospital of Wuhan University from January 31 to February 5, 2020 with confirmed COVID-19 were included. The primary outcomes were death and survival as of March 11. Demographics, vital signs, comorbidities and laboratory tests were collected and compared between those who died and survivors. Logistic regression analysis for prognostic factors was performed. Kaplan-Meier analysis was used to compare the estimated survival rate between patients with prolonged prothrombin time and normal prothrombin time.

Results: The total number of patients with confirmed COVID-19 who were enrolled was 213. The median age was 62 years, and 95 patients (44.6%) were men. Fifty-one patients were critical (23.9%), 79 patients were severe (37.1%) and 83 patients were moderate (39%). As of March 11, 2020, 99 patients were discharged (46.5%), 79 patients (37.1%) stayed in the hospital and 35 patients (16.2%) died. Median time to death was 6 (4-8) d, while median hospital stay was 32 (22-36) d in survivors ( < 0.001). More men ( = 0.002) and elderly patients ( < 0.001) were found in the group of those who died. The respiration rate at admission was higher in the group of those who died ( < 0.001). The incidences of hypertension ( = 0.028), cerebrovascular disease ( < 0.001), chronic kidney disease ( = 0.02) and chronic obstructive pulmonary disease ( < 0.001) were higher in the group of those who died. Platelet count was decreased in the group of those who died ( = 0.002) whereas prothrombin time ( < 0.001), activated partial thromboplastin time ( = 0.033), concentration of D-dimer ( < 0.001) and fibrin degradation products ( < 0.001) were increased in the group of those who died. Prothrombin time [odds ratio (OR): 2.19, = 0.004], respiration rate (OR: 1.223, < 0.001), age (OR: 1.074, < 0.001) and fibrin degradation products concentration (OR: 1.02, = 0.014) were predictors of death. The survival rate was significantly lower in patients with prolonged prothrombin time compare to those with normal prothrombin time ( < 0.001).

Conclusion: Prothrombin time, concentration of fibrin degradation products, respiration rate and age were predictive factors for clinical outcomes of COVID-19 patients.
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http://dx.doi.org/10.12998/wjcc.v8.i19.4370DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7559677PMC
October 2020

The Study on the Whole Stress-Strain Curves of Coral Fly Ash-Slag Alkali-Activated Concrete under Uniaxial Compression.

Materials (Basel) 2020 Sep 25;13(19). Epub 2020 Sep 25.

College of Civil Engineering, Anhui Jianzhu University, Hefei 230000, China.

This study aimed to research the whole stress-strain curves of coral Fly Ash-Slag Alkali-Activated Concrete (CAAC) in different strength grades. Fly ash-slag alkali-activated concrete was used as cementing materials to produce coral aggregate concrete. A monotonic loading test was conducted on the prismatic specimens to obtain elastic modulus (), Poisson's ratio () and the constitutive relationship of CAAC under uniaxial compression. When the strain of CAAC reached the maximum value, the specimen was split and damaged rapidly. As the strength grade increased, the ratio of residual stress () to peak stress () decreased in the range of 0.17-0.28. The of CAAC increased gradually, and increased to the peak value and then decreased. According to the test results, the constitutive equation of CAAC can be expressed by piecewise expression, which can better reflect all the experimental characteristics. It was also found that CAAC has many similar characteristics with coral concrete and lightweight aggregate concrete. To improve the strength and toughness of CAAC, some fibers, such as organic fiber, can be added to expand the application of CAAC in engineering projects.
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http://dx.doi.org/10.3390/ma13194291DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7579447PMC
September 2020

Effect of respiratory rehabilitation training on elderly patients with COVID-19: A protocol for systematic review and meta-analysis.

Medicine (Baltimore) 2020 Sep;99(37):e22109

Department of Pneumology, West China Hospital, Sichuan University, Chengdu, Sichun 610041, China.

Background: Patients with the Corona Virus Disease 2019 (COVID-19) often see their respiratory, physical, and psychological functions impaired to varying degrees, especially for the elderly patients. Timely respiratory rehabilitation intervention for such patients may improve their prognoses. However, its relative effectiveness has not been proved. Therefore, this study is purposed to determine the effect of respiratory rehabilitation on elderly patients with COVID-19.

Methods: This study will search the following electronic databases: Embase, MEDLINE, PubMed, Cochrane Library, China national knowledge infrastructure database, Wan Fang database, Chinese Science and Technology Periodical Database, and Chinese Biomedical Literature Database, with the retrieval period running from their inception to August 2020. All randomized controlled trials of respiratory rehabilitation training on elderly patients with COVID-19 are collected, and the data are selected and extracted independently according to the pre-designed inclusion/exclusion criteria. Cochrane bias risk assessment tool is used to evaluate the method quality and bias risk. All data analyses will be implemented by using Revman5.3 and Stata14 software.

Results: This study will make a high-quality and comprehensive evaluation of the efficacy of respiratory rehabilitation training on elderly patients with COVID-19.

Conclusion: The conclusions of this systematic review will deliver more convincing evidence.

Ethics And Dissemination: The private information collected from individuals will not be published. And this systematic review will also not involve impairing the participants' rights. Ethical approval is not required. The results may be published in a peer-reviewed journal or disseminated in relevant conferences.
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http://dx.doi.org/10.1097/MD.0000000000022109DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7489687PMC
September 2020

Hysteretic Behavior of Geopolymer Concrete with Active Confinement Subjected to Monotonic and Cyclic Axial Compression: An Experimental Study.

Materials (Basel) 2020 Sep 9;13(18). Epub 2020 Sep 9.

School of Civil Engineering, Central South University, Changsha 410075, China.

This paper investigated the performance of actively confined geopolymer concrete (GPC) through experiments. The mechanical properties of GPC under triaxial stress states were analyzed and discussed from the prospects of failure modes, axial peak stress and strain, monotonic and cyclic constitutive relationships. The experimental results demonstrated that the loading modes (monotonic loading and cyclic loading) had little effect on the failure mode and axial peak stress and strain. The improvement of the strength and ductility of GPC with the increase in confinement level was consistent with that of the conventional cement concrete while the strain enhancement of confined GPC was lower than that of confined conventional cement concrete at the same confinement level. The curves of the monotonic stress-strain and the envelop of cyclic compression were predicted through Mander's model with good accuracy. The unloading/reloading models proposed by Lokuge were modified and the predicted cyclic hysteresis curves for actively confined GPC were in good agreement with the cyclic compression results. Findings from this study provide references for the application of geopolymer concrete.
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http://dx.doi.org/10.3390/ma13183997DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7559876PMC
September 2020

Profiles of liver function abnormalities in elderly patients with Coronavirus Disease 2019.

Int J Clin Pract 2021 Mar 28;75(3):e13632. Epub 2020 Dec 28.

Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, People's Republic of China.

Background: The profiles of liver function abnormalities in COVID-19 patients need to be clarified.

Methods: In this retrospective study, consecutive COVID-19 patients over 60 years old in Renmin Hospital of Wuhan University from January 1 to February 6 were included. Data of demographics, clinical characteristics, comorbidities, laboratory tests, medications and outcomes were collected and analysed. Sequential alterations of serum alanine aminotransferase (ALT) were monitored.

Results: A total of 330 patients were included and classified into two groups with normal (n = 234) or elevated ALT (n = 96). There were fewer females (40.6% vs 54.7%, P = .020) and more critical cases (30.2% vs 19.2%, P = .026) in patients with elevated ALT compared with the normal group. Higher levels of bacterial infection indices (eg, white blood cell count, neutrophil count, C-reactive protein and procalcitonin) were observed in the elevated group. Spearman correlation showed that both ALT and AST levels were positively correlated with those indices of bacterial infection. No obvious effects of medications on ALT abnormalities were found. In patients with elevated ALT, most ALT elevations were mild and transient. 59.4% of the patients had ALT concentrations of 41-100 U/L, while only a few patients (5.2%) had high serum ALT concentrations above 300 U/L. ALT elevations occurred at 13 (10-17) days and recovered at 28 (18-35) days from disease onset. For most patients, the elevation of serum ALT levels occurred at 6-20 days after disease onset and reached their peak values within a similar time frame. The recovery of serum ALT levels to normal frequently occurred at 16-20 days or 31-35 days after disease onset.

Conclusions: Liver function abnormalities were observed in 29.1% of elderly people COVID-19 patients, which were slightly and transient in most cases. Liver function abnormalities in COVID-19 may be correlated with bacterial infection.
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http://dx.doi.org/10.1111/ijcp.13632DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7435518PMC
March 2021

Weighed Gene Coexpression Network Analysis Screens the Potential Long Noncoding RNAs and Genes Associated with Progression of Coronary Artery Disease.

Comput Math Methods Med 2020 6;2020:8183420. Epub 2020 Jul 6.

Department of Cardiology, Luotian Wanmizhai Hospital, Huanggang 438600, China.

Background: Coronary artery disease (CAD) is a type of heart disease with a high morbidity rate. This study is aimed at identifying potential biomarkers closely related to the progression of CAD.

Materials And Methods: A microarray dataset of GSE59867 was downloaded from a public database, Gene Expression Omnibus, which included 46 cases of stable CAD without a history of myocardial infarction (MI), 30 cases of MI without heart failure (HF), and 34 cases of MI with HF. Differentially expressed long noncoding RNAs (DElncRNAs) and mRNAs (DEmRNAs) were identified by the limma package, and functions of DEmRNAs were annotated by Gene Ontology and KEGG pathways. In addition, weighed gene coexpression network analysis (WGCNA) was used to construct a coexpression network of DEmRNAs, and a disease-related lncRNAs-mRNAs-pathway network was constructed. Finally, the datasets of GSE61145 and GSE57338 were used to verify the expression levels of the above highly correlated candidates.

Results: A total of 2362 upregulated mRNAs and 2816 downregulated mRNAs, as well as 235 upregulated lncRNAs and 113 downregulated lncRNAs were screened. These genes were significantly enriched in "cytokine-cytokine receptor interaction," "RIG-I-like receptor signaling pathway," and "natural killer cell-mediated cytotoxicity." Five modules including 1201 DEmRNAs were enriched in WGCNA. A coexpression network including 19 DElncRNAs and 413 DEmRNAs was constructed. These genes were significantly enriched in "phosphatidylinositol signaling system," "insulin signaling pathway," and "MAPK signaling pathway". Disease-related gene-pathway network suggested in "insulin signaling pathway," in "phosphatidylinositol signaling system," and in "MAPK signaling pathway" were involved in MI.

Conclusion: , , and were revealed to be CAD progression-associated genes by WGCNA coexpression network analysis.
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http://dx.doi.org/10.1155/2020/8183420DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7361886PMC
May 2021

Diffusion tensor imaging of the C1-C3 dorsal root ganglia and greater occipital nerve for cervicogenic headache.

Korean J Pain 2020 Jul;33(3):275-283

Department of Pain, Affiliated Hospital of North Sichuan Medical College, Nanchong, China.

Background: Previous studies showed neurography and tractography of the greater occipital nerve (GON). The purpose of this study was determining diffusion tensor imaging (DTI) parameters of bilateral GONs and dorsal root ganglia (DRG) in unilateral cervicogenic headache as well as the grading value of DTI for severe headache. The correlation between DTI parameters and clinical characteristics was evaluated.

Methods: The fractional anisotropy (FA) and apparent diffusion coefficient (ADC) values in bilateral GONs and cervical DRG (C2 and C3) were measured. Grading values for headache severity was calculated using a receiver operating characteristics curve. The correlation was analyzed with Pearson's coefficient.

Results: The FA values of the symptomatic side of GON and cervical DRG (C2 and C3) were significantly lower than that of the asymptomatic side (all the < 0.001), while the ADC values were significantly higher ( = 0.003, < 0.001, and = 0.003, respectively). The FA value of 0.205 in C2 DRG was considered the grading parameter for headache severity with sensitivity of 0.743 and specificity of 0.999 ( < 0.001). A negative correlation and a positive correlation between the FA and ADC value of the GON and headache index (HI; r = -0.420, = 0.037 and r = 0.531, = 0.006, respectively) was found.

Conclusions: DTI parameters in the symptomatic side of the C2 and C3 DRG and GON were significantly changed. The FA value of the C2 DRG can grade headache severity. DTI parameters of the GON significantly correlated with HI.
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http://dx.doi.org/10.3344/kjp.2020.33.3.275DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7336345PMC
July 2020

Advanced glycation end products inhibit the osteogenic differentiation potential of adipose-derived stem cells by modulating Wnt/β-catenin signalling pathway via DNA methylation.

Cell Prolif 2020 Jun 28;53(6):e12834. Epub 2020 May 28.

Department of Oral and Maxillofacial Surgery, The Affiliated Stomatology Hospital of Southwest Medical University, Luzhou, China.

Objectives: Advanced glycation end products (AGEs) are considered a cause of diabetic osteoporosis. Although adipose-derived stem cells (ASCs) are widely used in the research of bone regeneration, the mechanisms of the osteogenic differentiation of ASCs from diabetic osteoporosis model remain unclear. This work aimed to investigate the influence and the molecular mechanisms of AGEs on the osteogenic potential of ASCs.

Materials And Methods: Enzyme-linked immunosorbent assay was used to measure the change of AGEs in diabetic osteoporotic and control C57BL/6 mice. ASCs were obtained from the inguinal fat of C57BL/6 mice. AGEs, 5-aza2'-deoxycytidine (5-aza-dC) and DKK-1 were used to treat ASCs. Real-time cell analysis and cell counting kit-8 were used to monitor the proliferation of ASCs within and without AGEs. Real-time PCR, Western blot and Immunofluorescence were used to analyse the genes and proteins expression of osteogenic factors, DNA methylation factors and Wnt/β-catenin signalling pathway among the different groups.

Results: The AGEs and DNA methylation were increased in the adipose and bone tissue of the diabetic osteoporosis group. Untreated ASCs had higher cell proliferation activity than AGEs-treatment group. The expression levels of osteogenic genes, Opn and Runx2, were lower, and mineralized nodules were less in AGEs-treatment group. Meanwhile, DNA methylation was increased, and the Wnt signalling pathway markers, including β-Catenin, Lef1 and P-GSK-3β, were inhibited. After treatment with 5-aza-dC, the osteogenic differentiation capacity of ASCs in the AGEs environment was restored and the Wnt signalling pathway was activated during this process.

Conclusions: Advanced glycation end products inhibit the osteogenic differentiation ability of ASCs by activating DNA methylation and inhibiting Wnt/β-catenin pathway in vitro. Therefore, DNA methylation may be promising targets for the bone regeneration of ASCs with diabetic osteoporosis.
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http://dx.doi.org/10.1111/cpr.12834DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7309593PMC
June 2020

Modified Tragus Edge and Transmasseteric Anteroparotid Approach for Intracapsular and Condylar Neck Fractures.

J Craniofac Surg 2020 Sep;31(6):1822-1826

Department of Oral and Maxillofacial Surgery.

The aim of this study was to evaluate the effects of protecting the facial nerve and reducing the scar visibility using a modified tragus edge and transmasseteric anteroparotid approach compared to classic preauricular approach for intracapsular and condylar neck fractures. This retrospective study included 64 patients (78 sides) who underwent surgical treatment for intracapsular or condylar neck fractures from January 2014 to June 2018. Patients were divided into the experimental group (treated via a modified tragus edge and transmasseteric anteroparotid approach), and the control group (treated via the classical preauricular approach). Therapeutic outcome assessment parameters included facial nerve injury, salivary fistulae, wound infection, restricted mouth opening, postoperative occlusion disorders, and scar visibility. In the control group, there were 3 cases of facial nerve injuries and 2 cases of salivary fistulae. One case of temporary facial nerve injury occurred in the experimental group, with complete recovery within 1 month. The scars were less visible in the experimental group than in the control group. These results suggest that a modified tragus edge and transmasseteric anteroparotid approach reduced the incidences of facial nerve injuries, minimized the scar visibility, improved exposure of the operative site and fixation of titanium screws or plates, and did not increase the frequency of other complications.
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http://dx.doi.org/10.1097/SCS.0000000000006470DOI Listing
September 2020

Application of Nanomaterials in Regulating the Fate of Adipose-derived Stem Cells.

Curr Stem Cell Res Ther 2021 ;16(1):3-13

Department of Oral and Maxillofacial Surgery, The Affiliated Stomatology Hospital of Southwest Medical University, Luzhou 646000, China.

Adipose-derived stem cells are adult stem cells which are easy to obtain and multi-potent. Stem-cell therapy has become a promising new treatment for many diseases, and plays an increasingly important role in the field of tissue repair, regeneration and reconstruction. The physicochemical properties of the extracellular microenvironment contribute to the regulation of the fate of stem cells. Nanomaterials have stable particle size, large specific surface area and good biocompatibility, which has led them being recognized as having broad application prospects in the field of biomedicine. In this paper, we review recent developments of nanomaterials in adipose-derived stem cell research. Taken together, the current literature indicates that nanomaterials can regulate the proliferation and differentiation of adipose-derived stem cells. However, the properties and regulatory effects of nanomaterials can vary widely depending on their composition. This review aims to provide a comprehensive guide for future stem-cell research on the use of nanomaterials.
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http://dx.doi.org/10.2174/1574888X15666200502000343DOI Listing
January 2021

Coronavirus disease 2019 in elderly patients: Characteristics and prognostic factors based on 4-week follow-up.

J Infect 2020 06 30;80(6):639-645. Epub 2020 Mar 30.

Department of Cardiology, Renmin Hospital of Wuhan University, 238 Jiefang Road, Wuchang, Wuhan 430060, People's Republic of China; Cardiovascular Research Institute, Wuhan University, Wuhan, People's Republic of China; Hubei Key Laboratory of Cardiology, Wuhan, People's Republic of China. Electronic address:

Objective: To investigate the characteristics and prognostic factors in the elderly patients with COVID-19.

Methods: Consecutive cases over 60 years old with COVID-19 in Renmin Hospital of Wuhan University from Jan 1 to Feb 6, 2020 were included. The primary outcomes were death and survival till March 5. Data of demographics, clinical features, comorbidities, laboratory tests and complications were collected and compared for different outcomes. Cox regression was performed for prognostic factors.

Results: 339 patients with COVID-19 (aged 71±8 years,173 females (51%)) were enrolled, including 80 (23.6%) critical, 159 severe (46.9%) and 100 moderate (29.5%) cases. Common comorbidities were hypertension (40.8%), diabetes (16.0%) and cardiovascular disease (15.7%). Common symptoms included fever (92.0%), cough (53.0%), dyspnea (40.8%) and fatigue (39.9%). Lymphocytopenia was a common laboratory finding (63.2%). Common complications included bacterial infection (42.8%), liver enzyme abnormalities (28.7%) and acute respiratory distress syndrome (21.0%). Till Mar 5, 2020, 91 cases were discharged (26.8%), 183 cases stayed in hospital (54.0%) and 65 cases (19.2%) were dead. Shorter length of stay was found for the dead compared with the survivors (5 (3-8) vs. 28 (26-29), P < 0.001). Symptoms of dyspnea (HR 2.35, P = 0.001), comorbidities including cardiovascular disease (HR 1.86, P = 0.031) and chronic obstructive pulmonary disease (HR 2.24, P = 0.023), and acute respiratory distress syndrome (HR 29.33, P < 0.001) were strong predictors of death. And a high level of lymphocytes was predictive of better outcome (HR 0.10, P < 0.001).

Conclusions: High proportion of severe to critical cases and high fatality rate were observed in the elderly COVID-19 patients. Rapid disease progress was noted in the dead with a median survival time of 5 days after admission. Dyspnea, lymphocytopenia, comorbidities including cardiovascular disease and chronic obstructive pulmonary disease, and acute respiratory distress syndrome were predictive of poor outcome. Close monitoring and timely treatment should be performed for the elderly patients at high risk.
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http://dx.doi.org/10.1016/j.jinf.2020.03.019DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7118526PMC
June 2020

Antioxidative and Angiogenesis-Promoting Effects of Tetrahedral Framework Nucleic Acids in Diabetic Wound Healing with Activation of the Akt/Nrf2/HO-1 Pathway.

ACS Appl Mater Interfaces 2020 Mar 28;12(10):11397-11408. Epub 2020 Feb 28.

State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, P. R. China.

Currently available strategies show limited effects in preventing morbidity and disability from chronic diabetic wounds. Ideal vascularization is indispensable for better restoration and prognosis of diabetic wounds. This study aims to investigate the role of tetrahedral framework nucleic acids (tFNAs) in the process of angiogenesis during diabetic wound healing and the underlying mechanism. The in vitro results showed that tFNAs treatment enhanced the formation of a vessel-like structure that was inhibited by advanced glycation end products (AGEs). Positive variations were detected in aspects of cell viability, migratory ability, nitric oxide (NO) levels, and vascular endothelial growth factor-A (VEGF-A) expression. In addition, high reactive oxygen species (ROS) levels and gene expressions relevant to oxidative damage and inflammation in diabetic human umbilical vein endothelial cells (HUVECs) were attenuated by tFNAs. As for the underlying mechanism, the p-Akt/total Akt ratio, nuclear factor erythroid 2-related factor 2 (Nrf2) levels, and heme oxygenase-1 (HO-1) levels were higher in diabetic HUVECs treated with tFNAs. In vivo experiments showed that tFNAs facilitated diabetic wound healing by accelerating vascularization, epithelialization, collagen deposition, and collagen alignment. In conclusion, tFNAs could protect endothelial cell function, reduce inflammation, and impede oxidative damage through their antioxidant activity via the Akt/Nrf2/HO-1 signaling pathway. The application of tFNAs may pave the way for better healing of diabetic wounds.
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http://dx.doi.org/10.1021/acsami.0c00874DOI Listing
March 2020

Microglia mediate forgetting via complement-dependent synaptic elimination.

Science 2020 02;367(6478):688-694

Institute of Neuroscience and Department of Neurology of the Second Affiliated Hospital, NHC and CAMS Key Laboratory of Medical Neurobiology, Zhejiang University School of Medicine, Hangzhou 310058, China.

Synapses between engram cells are believed to be substrates for memory storage, and the weakening or loss of these synapses leads to the forgetting of related memories. We found engulfment of synaptic components by microglia in the hippocampi of healthy adult mice. Depletion of microglia or inhibition of microglial phagocytosis prevented forgetting and the dissociation of engram cells. By introducing CD55 to inhibit complement pathways, specifically in engram cells, we further demonstrated that microglia regulated forgetting in a complement- and activity-dependent manner. Additionally, microglia were involved in both neurogenesis-related and neurogenesis-unrelated memory degradation. Together, our findings revealed complement-dependent synapse elimination by microglia as a mechanism underlying the forgetting of remote memories.
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http://dx.doi.org/10.1126/science.aaz2288DOI Listing
February 2020

Evaluation of structurally different brominated flame retardants interacting with the transthyretin and their toxicity on HepG2 cells.

Chemosphere 2020 May 24;246:125749. Epub 2019 Dec 24.

Key Laboratory of Analytical Chemistry of the State Ethnic Affairs Commission, College of Chemistry and Materials Science, South-Central University for Nationalities, Wuhan, Hubei, 430074, China. Electronic address:

Brominated flame retardants (BFRs) are found at quantifiable levels in both humans and wildlife and may potentially cause a health risk. For BFRs and their derivatives, limited information regarding the relationship among the structure, binding affinity to the target protein and toxicity is currently available. In the present work, representative BFRs with different hydroxyl- or bromo-substituents, namely 2, 2', 4, 4'-tetrabromodiphenyl ether (BDE-47), 3-hydroxy-2, 2', 4, 4'-tetrabromodiphenyl ether (3-OH-BDE-47) and tetrabromobisphenol A (TBBPA), were selected to investigate the interactions with transthyretin (TTR) by electrospray ionization mass spectrometry (ESI-MS) and cytotoxicity on HepG2 cells. It was noted that BDE-47 had a weak binding affinity to TTR, while 3-OH-BDE-47 and TBBPA had a stronger binding affinity than BDE-47 and thyroxine (T4). Hence, 3-OH-BDE-47 and TBBPA could affect the binding of TTR with its native ligand T by competitive binding to TTR, even at equal concentrations, which might be associated with BFR toxicity of endocrine disruption. Negative cooperativity was found for 3-OH-BDE-47 and TBBPA binding to TTR, similar to T with a well-established negatively cooperative binding mechanism. The tendency of toxic effects on HepG2 cells for these three BFRs was, 3-OH-BDE-47 > TBBPA > BDE-47, and this order was in good agreement with the binding ability explored by ESI-MS experiments and molecular docking simulation. The observations obtained by this study demonstrate that the binding properties of these BFRs to TTR and their cytotoxicity are correlated with structure differentials and functional substituents.
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http://dx.doi.org/10.1016/j.chemosphere.2019.125749DOI Listing
May 2020

Insight into the effect of the continuous testing and aging on the SO sensing characteristics of a YSZ (Yttria-stabilized Zirconia)-based sensor utilizing ZnGaO and Pt electrodes.

J Hazard Mater 2020 04 27;388:121772. Epub 2019 Nov 27.

State Key Laboratory on Integrated Optoelectronics, Key Laboratory of gas sensors, Jilin Province, College of Electronic Science and Engineering, Jilin University, 2699 Qianjin Street, Changchun, 130012, China. Electronic address:

In this paper, YSZ-based mixed potential SO sensor with ZnGaO and Pt electrodes was developed and the effect of the continuous testing and aging process on the sensing characteristics was discussed. The results showed that with this process the response of the sensor to SO performed an opposite direction to that in the sensor's initial state. The reason might be that the PtS produced at the Pt electrode increased the electrochemical catalytic activity of the Pt electrode, leading to the mixed potential of the Pt electrode higher than that of the ZnGaO electrode. XPS and EDS results proved that a lot of Pt and S were produced at Pt electrode after this process. Moreover, vulcanized sensor also performed similar sensing properties to the above aging sensor, which indicated that the produced PtS should be the reason that the sensor performed reverse deflection on sensing properties. In addition, the sensor after sulfuration can detect 0.05-500 ppm SO with the sensitivity being 5 mV/decade to 0.05-1 ppm and 41 mV/decade to 1-500 ppm. The sensor also had a reliable stability during the continuous measurement.
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http://dx.doi.org/10.1016/j.jhazmat.2019.121772DOI Listing
April 2020

Luminescent Biofunctional Collagen Mimetic Nanofibers.

ACS Omega 2019 Oct 27;4(15):16270-16279. Epub 2019 Sep 27.

State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, P. R. China.

Collagen has long been one of the top targets for biomimetic design due to its superior structural and functional properties. Significant progress has been achieved to construct self-assembling peptides to mimic the fibrous nanostructure of native collagen, while it is still very demanding to fabricate peptide assemblies that can recapitulate both structural and biofunctional features of collagen. Herein, collagen-like peptides have been synthesized to contain negatively charged amino acids as the binding groups of lanthanide ions and the integrin-binding motif GFOGER. The simultaneous inclusion of negatively charged amino acids in the middle as well as at both terminals drives the peptides to self-assemble to form well-ordered nanofibers with distinct periodic banding patterns specifically mediated by lanthanide ions. The aggregation tendency and the morphology of the final assembled materials for the peptides are modulated in a pH-cooperative manner, which well mimics the pH-dependent fibrillogenesis of Type I collagen. The utilization of lanthanide ions in the system not only offers a convenient external stimulus but also functionalizes assembled materials with excellent luminescent features. Most notably, the lanthanide-triggered peptide assembled nanomaterials possess good cell adhesion properties, which resemble the biological function of collagen. This peptide-Ln system provides a facile and potent strategy to generate nanofibers that mimic both the structural and functional properties of natural collagen. These novel pH-responsive, luminescent, and biofunctional collagen mimetic nanofibers open fascinating opportunities in the development of improved functional biomaterials in tissue engineering, drug delivery, and medical diagnostics.
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http://dx.doi.org/10.1021/acsomega.9b00740DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6787889PMC
October 2019

Photodynamic therapy for prostate cancer: a systematic review and meta-analysis.

Prostate Int 2019 Sep 27;7(3):83-90. Epub 2018 Dec 27.

Department of Radiology, Affiliated Hospital of North Sichuan Medical College, Nanchong City, Sichuan Province 637000, China.

Purpose: Photodynamic therapy (PDT) is an emerging focal treatment modality for prostate cancer. However, the efficacy, safety, and functional outcomes of PDT are not clear. We performed a meta-analysis of available single-arm studies and control trials which used PDT for prostate cancer.

Materials And Methods: We searched Pubmed, Embase, Ovid and the Cochrane library (until March,2018) for studies about PDT in patients with prostate cancer. The negative biopsy rate after PDT, PSA decreasing rate, pooled rate of functional outcome (IPSS or IIEF-5), and adverse events were analyzed.

Results: 14 studies containing 654 patients were included. Nine of the 14 included studies had evaluated a negative biopsy rate after PDT. The pooled rate was 55.0% (95.0% CI: 0.44-0.66, I = 85.7%). Twelve of the 14 included studies which evaluated PSA decreasing rate with the pooled rate of 35.0% (95.0% CI: 0.24-0.47, I = 88.7%). Six of the included studies evaluated IPSS with decreasing rate of 29.1% (95.0 % CI: 2.7%-55.5%, I = 96.9%). Five of the included studies evaluated IIEF-5 with decreasing rate of 14.9% (95.0% CI: 6.8%-23.0%, I = 44.2%). The most common adverse events were haematuria (28.1%, 95.0% CI: 17.1%-39.2%, I = 79.8%), erectile dysfunction (23.1%, 95.0% CI: 9.7%-36.5%, I = 87.7%), and dysuria (18.6%, 95.0% CI: 12.1%-25.0 %, I = 53.4 %).

Conclusions: The meta-analysis results shows that PDT for patients with prostate cancer can be considered as effective based on single-arm clinical trials. Meanwhile, this study reveals that there are not only low levels of side effect rates but also insignificant effect on both urinary and erectile function. However, more high-quality RCTs are needed to evaluate the comparative efficacy, safety, and functional outcomes of PDT for patients with prostate cancer.
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http://dx.doi.org/10.1016/j.prnil.2018.12.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6713795PMC
September 2019
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