Publications by authors named "Lan Wu"

408 Publications

Transcriptome analysis provides insights into the molecular mechanism of GhSAMDC involving in rapid vegetative growth and early flowering in tobacco.

Sci Rep 2022 Aug 10;12(1):13612. Epub 2022 Aug 10.

College of Biology and Agricultural Resources, Huanggang Normal University, Huanggang, 438000, Hubei, China.

In previous study, ectopic expression of GhSAMDC improved vegetative growth and early flowering in tobacco, which had been explained through changes of polyamine content, polyamines and flowering relate genes expression. To further disclose the transcript changes of ectopic expression of GhSAMDC in tobacco, the leaves from wild type and two transgenic lines at seedling (30 days old), bolting (60 days old) and flowering (90 days old) stages were performed for transcriptome analysis. Compared to wild type, a total of 938 differentially expressed genes (DEGs) were found to be up- or down-regulated in the two transgenic plants. GO and KEGG analysis revealed that tobacco of wild-type and transgenic lines were controlled by a complex gene network, which regulated multiple metabolic pathways. Phytohormone detection indicate GhSAMDC affect endogenous phytohormone content, ABA and JA content are remarkably increased in transgenic plants. Furthermore, transcript factor analysis indicated 18 transcript factor families, including stress response, development and flowering related transcript factor families, especially AP2-EREBP, WRKY, HSF and Tify are the most over-represented in those transcript factor families. In conclusion, transcriptome analysis provides insights into the molecular mechanism of GhSAMDC involving rapid vegetative growth and early flowering in tobacco.
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http://dx.doi.org/10.1038/s41598-022-18064-4DOI Listing
August 2022

Puerarin attenuates isoproterenol‑induced myocardial hypertrophy via inhibition of the Wnt/β‑catenin signaling pathway.

Mol Med Rep 2022 Oct 10;26(4). Epub 2022 Aug 10.

Graduate School, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, P.R. China.

Myocardial hypertrophy (MH) is an independent risk factor for cardiovascular disease, which in turn lead to arrhythmia or heart failure. Therefore, attention must be paid to formulation of therapeutic strategies for MH. Puerarin is a key bioactive ingredient isolated from genera of plants that is beneficial for the treatment of MH. However, its molecular mechanism of action has not been fully determined. In the present study, 40 µM puerarin was demonstrated to be a safe dose for human AC16 cells using Cell Counting Kit‑8 assay. The protective effects of puerarin against MH were demonstrated in AC16 cells stimulated with isoproterenol (ISO). These effects were characterized by a significant decrease in surface area of cells (assessed using fluorescence staining) and mRNA and protein expression levels of MH‑associated biomarkers, including atrial and brain natriuretic peptide, assessed using reverse transcription‑quantitative PCR and western blotting, as well as β‑myosin heavy chain mRNA expression levels. Mechanistically, western blotting demonstrated that puerarin inhibited activation of the Wnt signaling pathway. Puerarin also significantly decreased phosphorylation of p65; this was mediated via crosstalk between the Wnt and NF‑κB signaling pathways. An inhibitor (Dickkopf‑1) and activator (IM‑12) of the Wnt signaling pathway were used to demonstrate that puerarin‑mediated effects alleviated ISO‑induced MH via the Wnt signaling pathway. The results of the present study demonstrated that puerarin pre‑treatment may be a potential therapeutic strategy for preventing ISO‑induced MH and managing MH in the future.
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http://dx.doi.org/10.3892/mmr.2022.12822DOI Listing
October 2022

Multiple microplastics induced stress on anaerobic granular sludge and an effectively overcoming strategy using hydrochar.

Water Res 2022 Jul 25;222:118895. Epub 2022 Jul 25.

Centre for Technology in Water and Wastewater, School of Civil and Environmental Engineering, University of Technology Sydney, Sydney, NSW 2007, Australia. Electronic address:

Previous studies mostly focused on the responses of anaerobic granular sludge (AGS) to one kind of microplastics during wastewater treatment. However, a wide variety of microplastics has been detected in wastewater. The multiple microplastics induced stress on AGS and the effectively mitigating strategy still remain unavailable. Herein, this work comprehensively excavated the influences of multiple microplastics (i.e., polyethylene terephthalate (PET), polystyrene (PS), polyethylene (PE) and polypropylene (PP)) coexisting in the wastewater on AGS system from macroscopic to microcosmic aspects. Experimental results illustrated that microplastics decreased AGS granule size, increased cell inactivation and caused deteriorative methane recovery from wastewater. As such, this study then put great emphasis on proposing a mitigating strategy using hydrochar and disclosing the role of hydrochar in overcoming the stress induced by coexisting microplastics to AGS system. Physiological characterization and microbial community analysis demonstrated that hydrochar effectively mitigated the reductions in methane production by 50.6% and cell viability by 68.8% of microplastics-bearing AGS and reduced the toxicity of microplastics to microbial community in the AGS. Mechanisms investigation by fluorescence tagging and excitation emission matrix fluorescence spectroscopy with fluorescence regional integration (EEM-FRI) analysis revealed that hydrochar adsorbed/accumulated microplastics and enhanced microplastics-bearing AGS to secrete extracellular polymeric substance (EPS) with more humic acid generation, thus reducing the direct contact between microplastics and AGS. In addition, hydrochar weakened the AGS intracellular oxidative stress induced by microplastics, thereby completely eliminating the inhibition of microplastics on acidification efficiency of AGS, and partially mitigating the suppression on methanation.
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http://dx.doi.org/10.1016/j.watres.2022.118895DOI Listing
July 2022

Analysis of Ultrasonographic Characteristics of Early Hepatic Alveolar Echinococcosis.

Front Surg 2022 5;9:918138. Epub 2022 Jul 5.

Department of Ultrasound, The 969th Hospital of the PLA Joint Logistics Support Force, Hohhot, China.

Objective: This study aims to investigate the ultrasonographic characteristics of early hepatic alveolar echinococcosis (HAE) and improve the qualitative diagnostic ability of sonographers.

Methods: The data of 80 positive cases of HAE screened and diagnosed by ultrasonography and serum immunology (33 males and 44 females with a mean age of 44.12 ± 14.31 y) were used in the study. The imaging characteristics of the lesions (i.e., intrahepatic distribution, shape, size, echo, blood flow, and growth characteristics) were analyzed retrospectively, and the ultrasonographic characteristics of early lesions were discussed in combination with their basic pathological changes.

Results: Patients with lesions of the proliferative infiltration type accounted for 57.5% (46/80), the fibrous calcification type accounted for 26.25% (21/80), the necrotic liquefaction type accounted for 6.25% (5/80), and the mixed type accounted for 10% (8/80). Patients with lesions involving the right lobe and the left lobe accounted for 76.25% (61/80) and 11.25% (9/80), respectively, and with lesions involving both the left and right lobes accounted for 12.5% (10/80). There were statistically significant differences in diameter between all types of lesions ( = 88; F = 5.926 and = 0.004). Focal hyperechoic and diffuse heterogenous nodular changes were the main manifestations of early proliferative infiltration lesions.

Conclusion: Ultrasonography is extremely valuable in the diagnosis of early HAE. Capillary hemangioma-like changes and hailstorm sign are the main characteristics of early lesions of HAE, and calcification is a common concomitant sign.
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http://dx.doi.org/10.3389/fsurg.2022.918138DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9294286PMC
July 2022

Innate and Innate-like Effector Lymphocytes in Health and Disease.

J Immunol 2022 07;209(2):199-207

Department of Pathology, Microbiology and Immunology, Vanderbilt University Medical Center, Nashville, TN.

Lymphocytes can be functionally partitioned into subsets belonging to the innate or adaptive arms of the immune system. Subsets of innate and innate-like lymphocytes may or may not express Ag-specific receptors of the adaptive immune system, yet they are poised to respond with innate-like speed to pathogenic insults but lack the capacity to develop classical immunological memory. These lymphocyte subsets display a number of common properties that permit them to integrate danger and stress signals dispatched by innate sensor cells to facilitate the generation of specialized effector immune responses tailored toward specific pathogens or other insults. In this review, we discuss the functions of distinct subsets of innate and innate-like lymphocytes. A better understanding of the mechanisms by which these cells are activated in different contexts, their interactions with other immune cells, and their role in health and disease may inform the development of new or improved immunotherapies.
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http://dx.doi.org/10.4049/jimmunol.2200074DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9285656PMC
July 2022

Dielectric barrier discharge-accelerated one-pot synthesis of sulfur quantum dots for fluorescent sensing of lead ions and L-cysteine.

Chem Commun (Camb) 2022 Aug 2;58(62):8614-8617. Epub 2022 Aug 2.

Key Laboratory of Green Chemistry & Technology (MOE), College of Chemistry, Sichuan University, Chengdu, 610064, China.

Here, we report a novel method for the one-pot facile synthesis of sulfur quantum dots (SQDs) based on a dielectric barrier discharge (DBD)-accelerated HO etching strategy within merely 20 min. The formation mechanism of SQDs was investigated, with which an "ON-OFF-ON" fluorescence sensor was developed for the detection of Pb ions and L-cysteine.
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http://dx.doi.org/10.1039/d2cc02993fDOI Listing
August 2022

A 30,000-km journey by Apus apus pekinensis tracks arid lands between northern China and south-western Africa.

Mov Ecol 2022 Jun 29;10(1):29. Epub 2022 Jun 29.

State Key Laboratory of Biocontrol, School of Ecology, Sun Yat-Sen University, Guangzhou, 510275, Guangdong, China.

Background: As a widely distributed and aerial migratory bird, the Common Swift (Apus apus) flies over a wide geographic range in Eurasia and Africa during migration. Although some studies have revealed the migration routes and phenology of European populations, A. a. apus (from hereon the nominate apus), the route used by its East Asian counterpart A. a. pekinensis (from hereon pekinensis) remained a mystery.

Methods: Using light level geolocators, we studied the migration of adult pekinensis breeding in Beijing from 2014 to 2018, and analysed full annual tracks obtained from 25 individuals. In addition, we used the mean monthly precipitation to assess the seasonal variations in humidity for the distribution ranges of the nominate apus and pekinensis. This environmental variable is considered to be critically relevant to their migratory phenology and food resource abundance.

Results: Our results show that the swifts perform a round-trip journey of ca 30,000 km each year, representing a detour of 26% in autumn and 15% in spring compared to the shortest route between the breeding site in Beijing and wintering areas in semi-arid south-western Africa. Compared to the nominate apus, pekinensis experiences drier conditions for longer periods of time. Remarkably, individuals from our study population tracked arid habitat along the entire migration corridor leading from a breeding site in Beijing to at least central Africa. In Africa, they explored more arid habitats during non-breeding than the nominate apus.

Conclusions: The migration route followed by pekinensis breeding in Beijing might suggest an adaptation to semi-arid habitat and dry climatic zones during non-breeding periods, and provides a piece of correlative evidence indicating the historical range expansion of the subspecies. This study highlights that the Common Swift may prove invaluable as a model species for studies of migration route formation and population divergence.
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http://dx.doi.org/10.1186/s40462-022-00329-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9245314PMC
June 2022

Ultra-dense plasmonic nanogap arrays for reorientable molecular fluorescence enhancement and spectrum reshaping.

Nanoscale 2022 Jun 21. Epub 2022 Jun 21.

Institute for Integrative Nanosciences, Leibniz IFW Dresden, Helmholtzstraße 20, 01069 Dresden, Germany.

Understanding interactions between molecular transition and intense electromagnetic fields confined by plasmon nanostructures is of great significance due to their huge potential in fundamental cavity quantum electrodynamics and practical applications. Here, we report reorientable plasmon-enhanced fluorescence leveraging the flexibilities in densely-packed gold nanogap arrays by template-assisted depositions. By finely adjusting the symmetry of the unit structure, arrays of nanogaps along two nearly-orthogonal axes can be tailored collectively with spacing down to sub-10 nm on a single chip, facilitating distinct "inter-cell" and "intra-cell" plasmon couplings. Through engineering two sets of nanogaps, the varying hybridization-induced plasmonic bonding modes lead to adjustable splitting of the fluorescence emission peak with a width up to 81 nm and narrowing of linewidths up to a factor of 3. Besides, polarization anisotropy with a ratio up to 63% is obtained on the basis of spectrally separated local hotspots with discrepant oscillation directions. The developed plasmonic nanogap array is envisaged to provide a promising chip-scale, cost-effective platform for advancing fluorescence-based detection and emission technologies in both classical and quantum regimes.
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http://dx.doi.org/10.1039/d2nr01543aDOI Listing
June 2022

Effects of Altitude and Duration of Differing Levels of Hypoxic Exposure on Hypoxia-Inducible Factor-1α in Rat Tissues.

High Alt Med Biol 2022 06 3;23(2):173-184. Epub 2022 Jun 3.

Institute of Chemical Technology, Northwest Minzu University, Lanzhou, China.

Li, Xiao-lin, Wei-gang Wang, Mao-xing Li, Tian-long Liu, Xiu-yu Tian, and Lan Wu. Effects of altitude and duration of differing levels of hypoxic exposure on hypoxia-inducible factor-1α in rat tissues. . 23:173-184, 2022. This research aimed to evaluate the effects of hypoxia at different altitudes and durations on the expression of hypoxia-inducible factor-1α (HIF-1α) in rat tissues. A total of 72 Wistar rats were used to investigate the effect of hypoxia at different durations on rat tissues and 72 Wistar rats were used to investigate the effect of hypoxia at different altitudes. Hematoxylin and Eosin (HE) staining was performed to observe the pathological changes of hippocampus tissues, and the expression of HIF-1α of rats under conditions of hypoxia was detected by quantitative real-time polymerase chain reaction and western blotting. According to the pathological results, we found that the degree of the brain, lung, and heart damage and HIF-1α expression, showed an increasing trend as the altitude (1,500, 3,000, 4,500, 6,000, 7,500, and 8,000 m for 12 hours) and duration (0, 6, 12, 24, 36, and 72 hours at 7,500 m) of hypoxia increased. Although there is a significant difference at 8,000 m, considering model stability, animal ethics and cost, we chose 7,500 m as a fixed altitude during hypoxia at different durations. Compared with the normoxic group, the expression of HIF-1α mRNA in the 7,500 m significantly increased by 30.48%, 21.00%, and 12.62%, in brain, lung, and heart tissue ( < 0.01), and HIF-1α mRNA in the 72-hour hypoxic exposure group significantly increased by 52.58%, 20.39%, 27.88% in tissues ( < 0.05). Compared with the normoxic group, HIF-1α protein expressions in the 7,500 m significantly increased by 10.26%, 31.71%, and 13.33% in brain, lung, and heart tissue ( < 0.01,  < 0.01,  < 0.05), and HIF-1α protein expressions in the 72-hour hypoxic exposure group significantly increased by 18.89%, 22.89%, and 29.75% in tissues ( < 0.05). HIF-1α expression in the rat was correlated with altitude and duration of hypoxic exposure.
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http://dx.doi.org/10.1089/ham.2021.0100DOI Listing
June 2022

Curcumin nanoemulsions inhibit oral squamous cell carcinoma cell proliferation by PI3K/Akt/mTOR suppression and miR-199a upregulation: A preliminary study.

Oral Dis 2022 Jun 11. Epub 2022 Jun 11.

Shanghai Key Laboratory of Stomatology, College of Stomatology, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Jiao Tong University, Shanghai, China.

Background: Accumulating evidence indicates that curcumin (CUR) has anticancer properties in various cancers including oral squamous cell carcinoma (OSCC), but CUR is greatly restricted in clinical studies and applications due to its low bioavailability. Interestingly, the bioavailability of CUR was found to be significantly improved using loaded lipid nanoemulsions (NEs).

Objectives: To investigate the effect of CUR-NEs on cell proliferation of OSCC HSC-3 cells in vitro, and explore the potential mechanism of this effect in a preliminary study.

Results: CUR-NEs exhibited significantly cytotoxic effects on OSCC cells in a dose-dependent manner, compared with the control. The percentage of cells in proliferative phases (S + G2/M) was gradually decreased in a dose- or time-dependent manner caused by CUR-NEs. Moreover, CUR-NEs downregulated the protein expression of PI3K/Akt/mTOR and upregulated the expression of miR-199a that targeted PI3K in a dose- or time-dependent manner in OSCC cells. Importantly, CUR-NEs cloud effectively counteract the influence on cell proliferation of OSCC cells and the proliferative phases of cell cycle caused by miR-199a inhibitor a time-dependent manner.

Conclusions: This in vitro preliminary study indicated that CUR-NEs may be an effective therapeutic agent for OSCC. Such effects could be related to inhibition of OSCC cell proliferation by PI3K/Akt/mTOR suppression and miR-199a upregulation.
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http://dx.doi.org/10.1111/odi.14271DOI Listing
June 2022

CRISPR/Cas9-Mediated Targeted Mutagenesis of Promotes Flavonoid Biosynthesis in Tartary Buckwheat ().

Front Plant Sci 2022 12;13:879390. Epub 2022 May 12.

Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Artemisinin Research Center, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China.

The clustered regularly interspaced short palindromic repeat/CRISPR-associated protein 9 (CRISPR/Cas9) technology is an efficient genome editing tool used in multiple plant species. However, it has not been applied to Tartary buckwheat (), which is an important edible and medicinal crop rich in rutin and other flavonoids. is an R2R3-type MYB transcription factor that negatively regulates flavonoid biosynthesis in Tartary buckwheat. Here, the CRISPR/Cas9 system polycistronic tRNA-sgRNA (PTG)/Cas9 was employed to knock out the gene in Tartary buckwheat. Two single-guide RNAs (sgRNAs) were designed to target the second exon of the gene. Twelve transgenic hairy roots were obtained using mediated transformation. Sequencing data revealed that six lines containing six types of mutations at the predicted double-stranded break site were generated using sgRNA1. The mutation frequency reached 50%. A liquid chromatography coupled with triple quadrupole mass spectrometry (LC-QqQ-MS) based metabolomic analysis revealed that the content of rutin, catechin, and other flavonoids was increased in hairy root mutants compared with that of lines transformed with the empty vector. Thus, CRISPR/Cas9-mediated targeted mutagenesis of effectively increased the flavonoids content of Tartary buckwheat. This finding demonstrated that the CRISPR/Cas9 system is an efficient tool for precise genome editing in Tartary buckwheat and lays the foundation for gene function research and quality improvement in Tartary buckwheat.
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http://dx.doi.org/10.3389/fpls.2022.879390DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9133938PMC
May 2022

Identification of Andrographis Herba and its common products using mini-barcode.

Chin J Nat Med 2022 May;20(5):393-400

Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China. Electronic address:

Andrographis Herba, the aerial part of Andrographis paniculata (Burm. f.) Wall. ex Nees (Acanthaceae), has a wide geographic distribution and has been used for the treatment of fever, cold, inflammation, and other infectious diseases. In markets, sellers and buyers commonly inadvertently confuse with related species. In addition, most Chinese medicinal herbs are subjected to traditional processing procedures, such as steaming and boiling, before they are sold at dispensaries; therefore, it is very difficult to identify Andrographis Herba when it is processed into Chinese medicines. The identification of species and processed medicinal materials is a growing issue in the marketplace. However, conventional methods of identification have limitations, while DNA barcoding has received considerable attention as a new potential means to identify species and processed medicinal materials. In this study, 17 standard reference materials of A. paniculata, 2 standard decoctions, 27 commercial products and two adulterants were collected. Based on the ITS2 sequence, it could successfully identify A. paniculata and adulterants. Moreover, a nucleotide signature consisting of 71 bp was designed, this sequence is highly conserved and specific within A. paniculata while divergent among other species. Then, we used these new primers to amplify the nucleotide signature region from processed materials. In conclusion, the DNA barcoding method developed in the present study for authenticating A. paniculata is rapid and cost-effective. It can be used in the future to guarantee the quality of Andrographis Herba of each regulatory link for clinical use.
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http://dx.doi.org/10.1016/S1875-5364(22)60157-2DOI Listing
May 2022

Clinical Analysis of Color Doppler Ultrasound Diagnosis of Senile Cataract Based on Intelligent Processor.

Comput Math Methods Med 2022 27;2022:1037439. Epub 2022 Apr 27.

Daqing Oilfield General Hospital, Daqing 163001, China.

In order to improve the effect of on-the-spot diagnosis of senile cataract, this paper combines the intelligent processor to explore the application of color Doppler ultrasound in the clinical analysis of senile cataract. Moreover, this paper measures and calculates the diameter, perimeter, area, volume, or velocity of blood flow reflected by color Doppler ultrasound images. In addition, this paper adopts the measurement method provided by composite measurement to design the intelligent processor. Each measurement analysis package is defined as an independent class, and the data, properties, and methods are encapsulated in a class, which is beneficial to the modular design of the program and the overall management of the system. The experimental results verify that the intelligent processor proposed in this paper has a certain effect in the clinical analysis of color Doppler ultrasound diagnosis of senile cataract.
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http://dx.doi.org/10.1155/2022/1037439DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9068292PMC
May 2022

Efficacy and safety of phacoemulsification cataract surgery with prophylactic vitreous injection of bevacizumab in the treatment of retinopathy: a systematic review and meta-analysis.

Ann Palliat Med 2022 Apr;11(4):1421-1430

Department of Ophthalmology, Renmin Hospital of Wuhan University, Wuhan, China.

Background: For patients with cataracts and retinopathy, phacoemulsification can improve the progress of the disease to a certain extent. However, the efficacy of phacoemulsification alone is often not ideal, and may even aggravate the prognostic effects, such as macular edema and slow visual recovery. Thus, an increasing number of clinicians have explored comprehensive therapy involving phacoemulsification combined with drug therapy, which has led to some research progress. However, there is still great controversy about the combined treatment. In this study, we performed a literature meta-analysis to systematically evaluate the clinical efficacy and safety of prophylactic intravitreal injection of bevacizumab combined with phacoemulsification for the treatment of cataracts complicated with retinopathy.

Methods: We searched the PubMed, Web of Science, Embase, and MEDLINE databases for English language papers related to these studies factors published between January 2001 and January 2021. The retrieved articles were screened and the quality of the included studies was evaluated. Meta-analysis was performed and RevMan5.3 was used to calculate the enhanced mean difference (MD).

Results: Six articles were finally included in this meta-analysis, involving a total of 325 cases with cataracts and retinopathy. The results showed that the best-corrected visual acuity at 1 month [MD =-0.06; 95% confidence interval (CI): (-0.09, -0.03); P=0.0002] and 3 months [MD =-0.09; 95% CI: (-0.11, -0.07); P<0.00001] in the preventive vitreous injection bevacizumab intervention group were significantly better than those of the control group (P<0.1). However, the best-corrected visual acuity at 6 months [MD =-0.02; 95% CI: (-0.07, 0.03); P=0.39] was not significantly different between these two groups (P>0.05). In addition, the central macular thickness of the preventive vitreous injection bevacizumab intervention group at 1 month after treatment [MD =-37.07; 95% CI: (-45.87, -28.27); P<0.00001], 3 months [MD =-15.26; 95% CI: (-23.87, -6.66); P=0.0005], and 6 months [MD =-26.77; 95% CI: (-37.51, -16.04); P<0.00001] were significantly different to the control group (P<0.05).

Discussion: The results of this study showed that prophylactic intravitreal injection of bevacizumab combined with phacoemulsification is a safe and effective treatment for patients with cataracts and retinopathy within 6 months after treatment.
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http://dx.doi.org/10.21037/apm-22-222DOI Listing
April 2022

Alterations and Correlations of Gut Microbiota and Fecal Metabolome Characteristics in Experimental Periodontitis Rats.

Front Microbiol 2022 14;13:865191. Epub 2022 Apr 14.

Center for Evidence-Based and Translational Medicine, Zhongnan Hospital of Wuhan University, Wuhan, China.

Objectives: Periodontitis affects the progression of many diseases, while its detailed mechanism remains unclear. This study hopes to provide new ideas for exploring its mechanism by analyzing the gut microbiota and fecal metabolic characteristics of experimental periodontitis rats.

Methods: A total of 10 rats were randomly divided into ligature-induced experimental periodontitis (EP) group and healthy control group. After 4 weeks of the experiment, the feces of all rats were collected for sequencing through 16S ribosomal DNA (rDNA) sequencing technology and liquid chromatography-mass spectrometry (LC-MS).

Results: 16S rDNA sequencing results showed that the β-diversity of gut microbiota was significantly different between the EP and control group, and the levels of dominant genera were different. Compared with the control group, , , and were significantly enriched in EP, and , , were significantly decreased. Correlation analysis showed that exhibited the highest correlation within the genus. Of 3,488 qualitative metabolites, 164 metabolites were upregulated and 362 metabolites were downregulated in EP. Enrichment analysis showed that periodontitis significantly changed 45 positive/negative ion metabolic pathways. Five KEGG pathways, protein digestion and absorption, tyrosine metabolism, glycolysis/gluconeogenesis, niacin and nicotinamide metabolism, and oxidative phosphorylation, are enriched in both the microbiome and metabolome. Correlation analysis showed that the genera with significant differences in periodontitis were usually significantly correlated with more metabolites, such as , , , , and . The genera with the same changing trend tended to have a similar correlation with some certain metabolites. In addition, vitamin D2 and protoporphyrin IX have the most significant correlations with microorganisms.

Conclusion: Our study reveals that periodontitis alters gut microbiota and fecal metabolites. The correlation analysis of microbiota and metabolome provides a deeper understanding of periodontitis, and also provides a direction for the study of periodontitis affecting other diseases.
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http://dx.doi.org/10.3389/fmicb.2022.865191DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9048259PMC
April 2022

Jumps and stock market variance during the COVID-19 pandemic: Evidence from international stock markets.

Financ Res Lett 2022 Aug 21;48:102896. Epub 2022 Apr 21.

School of Economics and Management, Southwest Jiaotong University, Chengdu, China.

Based on the work of Buncic and Gisler (2017), this paper investigates whether the roles of jump components will change in forecasting the volatility of international equity markets during the COVID-19 pandemic. Interestingly, in contrast to the conclusions of Buncic and Gisler (2017), we find jump components of the international equity indices are useful to predict the international stock markets' volatility during the COVID-19 pandemic. Our study tries to provide new evidence of jump components in stock markets.
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http://dx.doi.org/10.1016/j.frl.2022.102896DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9021036PMC
August 2022

Evaluation of Four Commonly Used DNA Barcoding Loci for Species Identification.

Front Plant Sci 2022 7;13:860778. Epub 2022 Apr 7.

Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China.

plants have been used as medicinal plants for a long time in China. Traditional techniques such as morphological, microscopic, and chemical identification methods all have limitations in the species identification of . For the sake of drug safety, four DNA barcodes (, ITS, , and ) were assessed for Chinese plants using a total of 121 individuals from 33 species. Four criteria (The success rates of PCR amplification, DNA barcoding gap, DNA sequence similarity analysis and NJ tree clustering analysis) were used to evaluate the species identification ability of these four DNA barcodes. The results show that ITS had the highest efficiency in terms of PCR and sequencing and exhibited the most apparent inter- and intra-specific divergences and the highest species identification efficiency. There was no significant increase in species identification after combining the three cpDNA fragments with the ITS fragment. Considering the cost and experimental effectiveness, we recommend ITS as the core barcode for identifying Chinese plants.
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http://dx.doi.org/10.3389/fpls.2022.860778DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9021757PMC
April 2022

Isolation, Characterization, and Depolymerization of l-Cysteine Substituted Lignin.

Glob Chall 2022 Apr 3;6(4):2100130. Epub 2022 Mar 3.

State Key Laboratory of Pulp and Paper Engineering School of Light Industry and Engineering South China University of Technology Guangzhou 510640 China.

Lignin condensation reactions are hard to avoid or control during separation, which is a deterrent to lignin isolation and post-conversation, especially for the full utilization of lignocelluloses. Selective protection of β-aryl ether linkages in the isolation process is crucial to lignin valorization. Herein, a two-step acid/alkali separation method assisted with l-cysteine for lignin separation is developed, and the isolated l-cysteine lignins (LCLs) are comprehensively characterized by 2D NMR, P NMR, thioacidolysis, etc. Compared to the two-step control treatment, a much higher β-O-4 content is preserved without reducing the separation efficiency assisted by l-cysteine, which is also significantly higher than alkali lignin and kraft lignin. The results of hydrogenolysis show that LCLs generate a much higher monomer yield than that of control sample. Structural analysis of LCLs suggests that lignin condensation reaction, to some extent, is suppressed by adding l-cysteine during the two-step acid/alkali separation. Further, mechanistic studies using dimeric model compound reveals that l-cysteine may be the α-carbon protective agent in the two-step separation. The role of l-cysteine in the two-step lignin isolation method provides novel insights to the selective fractionation of lignin from biomass, especially for the full valorization of lignocellulosic biomass.
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http://dx.doi.org/10.1002/gch2.202100130DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8995711PMC
April 2022

Dual Inhibition of H3K9me2 and H3K27me3 Promotes Tumor Cell Senescence without Triggering the Secretion of SASP.

Int J Mol Sci 2022 Apr 1;23(7). Epub 2022 Apr 1.

The Key Laboratory of Molecular Epigenetics of Ministry of Education (MOE), Northeast Normal University, Changchun 130024, China.

Chemotherapy remains the most common cancer treatment. Although chemotherapeutic drugs induce tumor cell senescence, they are often associated with post-therapy tumor recurrence by inducing the senescence-associated secretory phenotype (SASP). Therefore, it is important to identify effective strategies to induce tumor cell senescence without triggering SASP. In this study, we used the small molecule inhibitors, UNC0642 (G9a inhibitor) and UNC1999 (EZH2 inhibitor) alone or in combination, to inhibit H3K9 and H3K27 methylation in different cancer cells. Dual inhibition of H3K9me2 and H3K27me3 in highly metastatic tumor cells had a stronger pro-senescence effect than either inhibitor alone and did not trigger SASP in tumor cells. Dual inhibition of H3K9me2 and H3K27me3 suppressed the formation of cytosolic chromatin fragments, which inhibited the cGAS-STING-SASP pathway. Collectively, these data suggested that dual inhibition of H3K9 and H3K27 methylation induced senescence of highly metastatic tumor cells without triggering SASP by inhibiting the cGAS-STING-SASP pathway, providing a new mechanism for the epigenetics-based therapy targeting H3K9 and H3K27 methylation.
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http://dx.doi.org/10.3390/ijms23073911DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8999616PMC
April 2022

Chemopreventive efficacy of salvianolic acid B phospholipid complex loaded nanoparticles against experimental oral carcinogenesis: implication of sustained drug release.

Ann Transl Med 2022 Mar;10(5):244

Department of Oral Mucosal Diseases, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai, China.

Background: Although we have previously demonstrated that phospholipid complex loaded nanoparticles (PLC-NPs) encapsulating salvianolic acid B (SAB) can enhance anticancer activity in head and neck cancer and precancerous cells , the chemopreventive efficacy of SAB-PLC-NPs (nano-SAB) remains unclear. Here, we aimed to investigate the efficacy of nano-SAB against experimental oral carcinogenesis.

Methods: Oral tongue carcinogenesis was induced in C57BL/6 mice through the administration of 4-nitroquinoline-N-oxide (4NQO, 100 µg/mL) in drinking water for 22 weeks. To preliminarily evaluate the effect of sustained drug release against oral carcinogenesis, free- or nano-SAB (16.6 mg/kg/d) was administered orally for 18 weeks, and the treatment was discontinued for the remaining 4 weeks.

Results: Histological evaluation revealed a significant (P<0.05) decrease in the incidence of carcinoma in free-SAB-treated (16.7%) and nano-SAB-treated (10.0%) mice compared to mice exposed to 4NQO alone (34.3%). A decrease in carcinoma growth rate was also observed in free-SAB-treated (12.2%) and nano-SAB-treated (5.5%) mice compared to the 4NQO-exposed group (18.3%), even after drug withdrawal for 4 weeks. Immunohistochemical analysis revealed that nano-SAB treatment effectively suppressed Ki-67, proliferative cell nuclear antigen (PCNA), and cyclin D1 expression in high-risk dysplastic lesions compared to free-SAB-treated and 4NQO-exposed groups (all P<0.05). Importantly, nano-SAB maintained low levels of Ki-67, PCNA, and cyclin D1 expression even after drug withdrawal for 4 weeks.

Conclusions: Together with our previous data, this study confirms that nano-SAB has superior chemopreventive efficacy by promoting more potent anti-proliferation and cell cycle arrest responses. These findings demonstrate the potential of SAB-PLC-NPs as promising chemopreventive agents for treating oral carcinogenesis.
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http://dx.doi.org/10.21037/atm-21-4457DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8987877PMC
March 2022

Sesquiterpene dimers from Chloranthus fortunei and their protection activity against acute lung injury.

Fitoterapia 2022 Jun 1;159:105191. Epub 2022 Apr 1.

School of pharmacy, Anhui Medical University, Hefei 230032, People's Republic of China. Electronic address:

Chloranthus fortunei (family Chloranthaceae), a perennial herb, widely distributed in south China with an altitude of 170-340 m. The whole plants were used as an anti-inflammatory agent for the treatment of cough, arthritis and tumor. Five previously unreported compounds fortulactones A-E were isolated from the aerial part of Chloranthus fortunei. Their structures were elucidated using 1D/2D NMR and HRESIMS and their absolute configuration were determined using the ECD excitron chirality method. All isolates were tested for inhibitory effects on the NO production of liposaccharide (LPS)-induced RAW 264.7 macropahges. The most potent compound 1 was further evaluated its protective activity against LPS stimulated A549 cells, the ELISA kits results showed the abnormal states of MDA and SOD were corrected to a certain extent. Meanwhile, the pro-inflammatory cytokine, such as TNF-α, IL-6 and IL-1β were also attenuated. In conclusion, these results showed that 1 exhibited therapeutic potential for ameliorating ALI.
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http://dx.doi.org/10.1016/j.fitote.2022.105191DOI Listing
June 2022

Alterations of gut microbiota diversity, composition and metabonomics in testosterone-induced benign prostatic hyperplasia rats.

Mil Med Res 2022 03 28;9(1):12. Epub 2022 Mar 28.

Center for Evidence-Based and Translational Medicine, Zhongnan Hospital of Wuhan University, Wuhan, 430071, China.

Background: Studies had shown many diseases affect the stability of human microbiota, but how this relates to benign prostatic hyperplasia (BPH) has not been well understood. Hence, this study aimed to investigate the regulation of BPH on gut microbiota composition and metabonomics.

Methods: We analyzed gut samples from rats with BPH and healthy control rats, the gut microbiota composition and metabonomics were detected by 16S rDNA sequencing and liquid chromatography tandem mass spectrometry (LC-MS/MS).

Results: High-throughput sequencing results showed that gut microbiota beta-diversity increased (P < 0.01) in the BPH group vs. control group. Muribaculaceae (P < 0.01), Turicibacteraceae (P < 0.05), Turicibacter (P < 0.01) and Coprococcus (P < 0.01) were significantly decreased in the BPH group, whereas that of Mollicutes (P < 0.05) and Prevotella (P < 0.05) were significantly increased compared with the control group. Despite profound interindividual variability, the levels of several predominant genera were different. In addition, there were no statistically significant differences in several bacteria. BPH group vs. control group: Firmicutes (52.30% vs. 57.29%, P > 0.05), Bacteroidetes (46.54% vs. 41.64%, P > 0.05), Clostridia (50.89% vs. 54.66%, P > 0.05), Ruminococcaceae (25.67% vs. 20.56%, P > 0.05). LC-MS/MS of intestinal contents revealed that differential metabolites were mainly involved in cellular processes, environmental information processing, metabolism and organismal systems. The most important pathways were global and overview maps, lipid metabolism, amino acid metabolism, digestive system and endocrine system. Through enrichment analysis, we found that the differential metabolites were significantly enriched in metabolic pathways, steroid hormone biosynthesis, ovarian steroidogenesis, biosynthesis of unsaturated fatty acids and bile secretion. Pearson correlation analysis (R = 0.94) showed that there was a strong correlation between Prevotellaceae, Corynebacteriaceae, Turicibacteraceae, Bifidobacteriaceae and differential metabolites.

Conclusion: Our findings suggested an association between the gut microbiota and BPH, but the causal relationship between the two groups is unclear. Thus, further studies are warranted to elucidate the potential mechanisms and causal relationships between BPH and gut microbiota.
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http://dx.doi.org/10.1186/s40779-022-00373-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8962033PMC
March 2022

Effects and Safety of Non-Pharmacological Therapies of Traditional Chinese Medicine for Coronary Heart Disease: An Overview of Systematic Reviews.

Evid Based Complement Alternat Med 2022 19;2022:8465269. Epub 2022 Mar 19.

Guangdong Provincial Hospital of Chinese Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China.

Objectives: Coronary heart disease (CHD) is currently the leading cause of human death. Non-pharmacological therapy of traditional Chinese medicine (NPTCM) is an important characteristic therapy of traditional Chinese medicine (TCM). Questions concerning the efficacy and safety of NPTCM-related interventions in patients with CHD led us to conduct this overview of systematic reviews (SRs).

Methods: The Cochrane Library, PubMed, Embase, EBSCO, Epistemonikos, PROSPERO, CNKI, Wanfang Database, and SinoMed were searched without language and publication status restriction from their inception to May 2021, along with grey literature sites and reference lists of included reviews. Systematic reviews comparing NPTCM/a combination of NPTCM and non-TCM interventions with non-TCM interventions/inactive controls for CHD were examined. Two reviewers independently screened titles, abstracts, and full-text articles, and completed data extraction and quality appraisal according to the predefined standards.

Results: In total, 1494 titles and abstracts and 66 full-text articles were screened, and a total of 12 SRs (11 with meta-analysis) were finally included. According to PRISMA 2020 checklist, more than 50% of reviews conformed to 80% of 54 items. Consistent evidence of effectiveness or harms across multiple outcomes based on more than one moderate quality review with meta-analysis was found for acupuncture and usual care plus acupuncture/Baduanjin/TCM exercise therapies/TCM emotional therapy. These interventions were mostly evaluated less than 6 months.

Conclusion: Acupuncture or acupuncture plus usual care could improve angina symptoms and ECG, and usual care plus Baduanjin/TCM exercise therapies could ameliorate health-related quality of life. Additionally, Baduanjin plus usual care could also improve psychological condition, and it as well as acupuncture could be safe due to no reports on adverse events related to these interventions. TCM emotional therapy plus usual care could benefit patients with CHD and depression.
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http://dx.doi.org/10.1155/2022/8465269DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8957469PMC
March 2022

Molecular Programming of NIR-IIb-Emissive Semiconducting Small Molecules for In Vivo High-Contrast Bioimaging Beyond 1500 nm.

Adv Mater 2022 May 8;34(19):e2201263. Epub 2022 Apr 8.

Center of Super-Diamond and Advanced Films (COSDAF), Department of Chemistry, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong, 000000, P. R. China.

Materials with long-wavelength second near-infrared (NIR-II) emission are highly desired for in vivo dynamic visualizating of microstructures in deep tissues. Herein, by employing an atom-programming strategy, a series of highly fluorescent semiconducting oligomers (SOMs) with tunable NIR-IIb emissions are developed for bioimaging applications. After self-assembly into nanoparticles (NPs), they show good brightness, high photostability, and satisfactory biocompatibility. The SOM NPs are applied as probes for high-resolution imaging of whole-body and hind-limb blood vessels, biliary tract, and bladder with their emissions over 1500 nm. This work demonstrates an atom-programming strategy for constructing semiconducting small molecules with enhanced NIR-II fluorescence for deep-tissue imaging, affording new insight for advancing molecular design of NIR-II fluorophores.
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http://dx.doi.org/10.1002/adma.202201263DOI Listing
May 2022

The spatial and temporal trends of severe periodontitis burden in Asia, 1990-2019: A population-based epidemiological study.

J Periodontol 2022 Mar 15. Epub 2022 Mar 15.

Center for Evidence-Based and Translational Medicine, Zhongnan Hospital of Wuhan University, Wuhan, Hubei Province, China.

Background: To investigate the long-term and spatial patterns of incidence, prevalence, and disability-adjusted life year (DALY) rates of severe periodontitis in Asia from 1990 to 2019, and to estimate the associations between disease burden and socioeconomic development using the Socio-Demographic Index (SDI).

Methods: Data were obtained from the global burden of disease study 2019. The average annual percent change (AAPC) was calculated to reflect temporal trends, spatial autocorrelation analysis was conducted to estimate the spatial characteristics, and spatial panel models were used to investigate the association between SDI and severe periodontitis burden.

Results: For Asia as a whole, the crude rates increased by 1.10% per year for incidence, 1.42% per year for prevalence, and 1.41% per year for DALY from 1990 to 2019. The age-standardized incidence, prevalence and DALY rates increased by 0.18%, 0.22%, and 0.23% per year, respectively. Spatially, the hot spots of age-standardized incidence, prevalence and DALY rates were located in Southern Asia, besides, these rates all showed increasing trends in most countries, and the increases were clustered in Southeastern Asia. Further, SDI showed a negative association with incidence (coef = -14.44; 95% CI: -24.63, -4.25) and prevalence (coef = -40.09; -51.81, -28.36), and a positive association with DALY rates (coef = 0.31; 0.23; 0.38).

Conclusions: Severe periodontitis poses a serious public health challenge in Asian countries with increasing temporal trends and substantial spatial inequalities. Effective geographically targeted public health interventions and strategies are needed to address the growing burden associated with severe periodontitis.
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http://dx.doi.org/10.1002/JPER.21-0625DOI Listing
March 2022

Application of Three-Dimensional Pseudocontinuous Arterial Spin Labeling Perfusion Imaging in the Brains of Children With Autism.

Front Neurol 2022 23;13:851430. Epub 2022 Feb 23.

Department of Radiology Children's Hospital of Chongqing Medical University, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Pediatrics, Chongqing, China.

Objective: To explore the application of three-dimensional pseudocontinuous arterial spin labeling (3D-PCASL) perfusion imaging in the brains of children with autism and to understand the characteristics of cerebral blood perfusion in children with autism.

Methods: A total of 320 children with autism (160 men and 160 women) aged between 2 and 18 years and 320 age- and sex-matched healthy children participated in the study. All children were scanned by 3.0 T magnetic resonance axial T1 fluid-attenuated inversion recovery (FLAIR), T2 FLAIR, 3D-T1, and 3D-PCASL sequences. After postprocessing, cerebral blood flow (CBF) values in each brain region of children with autism and healthy children at the same age were compared and analyzed. Furthermore, CBF characteristics in each brain region of autistic children at various ages were determined.

Results: The CBF values of the frontal lobe, hippocampus, temporal lobe, and caudate nucleus of children with autism are lower than those of healthy children ( < 0.05). Additionally, as the ages of children with autism increase, the number of brain regions with decreased CBF values gradually increases. A receiver operating characteristic (ROC) analysis results show that the CBF values of the frontal lobe, hippocampus, temporal lobe, and caudate nucleus can distinguish children with autism [area under the ROC curve (AUC) > 0.05, < 0.05].

Conclusion: The 3D-PCASL shows lower brain CBF values in children with autism.

Clinical Trial Registration: www.ClinicalTrials.gov, identifier: ChiCTR2000034356.
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http://dx.doi.org/10.3389/fneur.2022.851430DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8905523PMC
February 2022

Calcium peroxide pre-treatment improved the anaerobic digestion of primary sludge and its co-digestion with waste activated sludge.

Sci Total Environ 2022 Jul 7;828:154404. Epub 2022 Mar 7.

Centre for Technology in Water and Wastewater, University of Technology Sydney, School of Civil and Environmental Engineering, Sydney, NSW 2007, Australia. Electronic address:

Primary sludge (PS) and Waste activated sludge (WAS) as two main sludge streams in wastewater treatment plants are commonly anaerobically co-digested, which though may be differently affected by pretreatment. Previous work has found that calcium peroxide (CaO) pretreatment effectively enhanced anaerobic digestion of WAS. However, the feasibilities of this strategy on PS anaerobic digestion and co-digestion of WAS and PS are still unclear. Herein, this work provided new insights into these systems. Biomethane potential test demonstrated that CaO pretreatment at 0.02-0.26 g/g-volatile suspended solids (VSS) promoted anaerobic digestion of PS. Then the feasibility of CaO pretreatment for improving anaerobic co-digestion of PS and WAS mixture was confirmed, with the highest improvement in methane production, VSS destruction and sludge reduction being approximately 37.4%, 38.9% and 19.9%, achieved at 0.14 g/g-VSS of CaO. Process modelling analysis revealed that CaO pretreatment increased both degradable faction and actually degraded fraction in sludge mixture. The changes of sludge characteristics via pretreatment and key enzyme activity in sludge anaerobic co-digestion system demonstrated that increased CaO concentration resulted in increased soluble organics release from sludge mixture in the pretreatment stage and inhibited activity of coenzyme F responsible for methanogenesis. Further mechanism investigation disclosed that OH, O and OH were main contribution factors, and the order of their contributions were OH >O >OH. This work laid the theoretical foundation and provided guidance for the practical application of CaO pre-treatment technology.
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http://dx.doi.org/10.1016/j.scitotenv.2022.154404DOI Listing
July 2022

Management of oral leukoplakia by ablative fractional laser-assisted photodynamic therapy: A 3-year retrospective study of 48 patients.

Lasers Surg Med 2022 07 7;54(5):682-687. Epub 2022 Mar 7.

Department of Oral Mucosal Diseases, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine; College of Stomatology, Shanghai Jiao Tong University; National Center for Stomatology; National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai, China.

Objectives: This study aimed to review the results of oral leucoplakia (OL) using ablative fractional laser-assisted photodynamic therapy (AFL-PDT) and to further evaluate the risk factors for recurrence and malignant transformation.

Materials And Methods: Forty-eight patients diagnosed with OL using histopathology were enrolled in this study. All patients received one session of AFL-PDT. Therapeutic efficacy was evaluated 1 month posttreatment. Follow-up was scheduled every 3 months in the first year and every 6 months thereafter.

Results: An overall positive response rate of 87.5% (42/48) was achieved, including 62.5% (30/48) complete responses and 25.0% (12/48) partial responses. During the 3-year follow-up period, the recurrence and malignant transformation rates were 37.5% (18/48) and 8.3% (4/48), respectively. Lesions on gingiva/palate seemed to be associated with recurrence (p < 0.001; odds ratio [OR]: 1.64, 95% confidence interval [CI]: 1.13-2.37). The severity of epithelial dysplasia (p = 0.02; OR: 2.93, 95% CI: 1.96-4.42) and recurrence (p = 0.016; OR: 3.14, 95% CI: 2.04-4.84) were associated with a predisposition to malignant transformation.

Conclusions: AFL-PDT is an effective management of OL, but requires close follow-up. OL lesions on the gingiva/palate are predisposed to recurrence. OLs that recur with moderate/severe epithelial dysplasia have a higher risk of transforming into oral squamous cell carcinoma.
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http://dx.doi.org/10.1002/lsm.23534DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9314786PMC
July 2022

Visual Corneal Photomechanical Analysis Is Combined with the Pentacam Correlation Index for the Diagnosis of Cone Corneas at Different Stages of Progression.

J Healthc Eng 2022 21;2022:4682901. Epub 2022 Feb 21.

Department of Ophthalmology, Taikang Tongji (Wuhan) Hospital, Sixin Road North, Wuhan 430050, China.

Cone-shaped corneas are blinding eye diseases characterised by dilated and thinning corneal tissue and forward conical protrusions, most often in adolescents. Early detection and intervention can prevent further dilatation of the cornea. The prevailing examination methods and techniques are not difficult to diagnose clinical cone corneas, but there are limitations in the diagnosis of early cone corneas (static cone corneas and subclinical cone corneas). In this study, we investigated the diagnostic value of the combination of these two instruments in the diagnosis of clinical cone corneas and subclinical cone corneas by performing the Pentacam and Corvis ST examinations in healthy eyes and dystrophic cone corneas. This study provides a theoretical basis for early cone cornea screening and diagnosis. The analysis revealed that only TP, SPA1, ARTh, and bIOP were normally distributed among all included parameters, and only bIOP was normally distributed with equal variance. TBI and CBI indices have a certain sensitivity and specificity for the diagnosis of subclinical cone corneas, with a strong diagnostic ability, and can be used for screening and early diagnosis of cone corneas.
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http://dx.doi.org/10.1155/2022/4682901DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8885271PMC
May 2022
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