Publications by authors named "Lan Rong"

38 Publications

Brain functional changes in patients with Crohn's disease: A resting-state fMRI study.

Brain Behav 2021 08 14;11(8):e2243. Epub 2021 Jun 14.

Department of Radiology, Jing'an District Centre Hospital of Shanghai, Fudan University, Shanghai, China.

Background: Crohn's disease (CD) is a chronic recurrent intestinal inflammatory disease, often accompanied by poor adaptation and excessive stress response. However, the potential neurological mechanisms of these symptoms have not yet been studied in-depth.

Objective: To investigate alterations in brain activity in patients with Crohn's disease and study the relationship between altered regions and clinical indices.

Methods: A total of 15 CD patients and 26 matched healthy controls were recruited. All participants underwent fMRI scans. The amplitude of low-frequency fluctuations (ALFF) and regional homogeneity (ReHo) assessed differences in spontaneous regional brain activity. Differences between the groups were selected as seeds for functional connectivity (FC) analyses. Correlations between disease duration and ALFF/ReHo/FC values in abnormal regions were analyzed.

Results: Patients with CD had significantly higher ALFF values in the left superior frontal gyrus, anterior cingulate cortex, and supplementary motor area, and lower values in the left hippocampus. They also had higher ReHo values in the left anterior cingulate cortex, supplementary motor area, putamen, and the bilateral superior frontal gyri. FC strength in the left precentral and middle temporal gyri was found to be increased when the left superior frontal gyrus was used as the seed point. FC strength was also observed to be increased in the left postcentral, middle frontal gyri, inferior frontal orbital cortex, and right rolandic operculum when the left anterior cingulate cortex was used as the seed point.

Conclusion: CD demonstrated abnormal neural activity and FC in various regions primarily associated with emotional, pain and cognitive-related functions, which provides more information to further understand the neural mechanisms of the disease.
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http://dx.doi.org/10.1002/brb3.2243DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8413760PMC
August 2021

Biological functions of NLRP3 inflammasome: A therapeutic target in inflammatory bowel disease.

Cytokine Growth Factor Rev 2021 08 14;60:61-75. Epub 2021 Mar 14.

Experiment Center for Science and Technology, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China. Electronic address:

Cases of inflammatory bowel disease (IBD), a debilitating intestinal disorder with complex pathological mechanisms, have been increasing in recent years, straining the capacity of healthcare systems. Thus, novel therapeutic targets and innovative agents must be developed. Notably, the NLRP3 inflammasome is upregulated in patients with IBD and/or in animal experimental models. As an innate immune supramolecular assembly, the NLRP3 inflammasome is persistently activated during the pathogenesis of IBD by multiple stimuli. Moreover, this protein complex regulates pro-inflammatory cytokines. Thus, targeting this multiprotein oligomer may offer a feasible way to relieve IBD symptoms and improve clinical outcomes. The mechanisms by which the NLRP3 inflammasome is activated, its role in IBD pathogenesis, and the drugs administered to target this protein complex are reviewed herein. This review establishes that the use of inflammasome-targeting drugs are effective for IBD treatment. Moreover, this review suggests that the value and potential of naturally sourced or derived medicines for IBD treatment must be recognized and appreciated.
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http://dx.doi.org/10.1016/j.cytogfr.2021.03.003DOI Listing
August 2021

Macrophage polarization: an effective approach to targeted therapy of inflammatory bowel disease.

Expert Opin Ther Targets 2021 Mar 17;25(3):191-209. Epub 2021 Mar 17.

School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai, China.

: Inflammatory bowel disease (IBD) is a systemic disease with immune abnormalities that can affect the entire digestive tract. A high percentage of patients with IBD are unresponsive to current pharmacological agents, hence the need exists for novel therapeutic approaches. There is compelling evidence that macrophage polarization plays a key role in the remission of IBD patients and that it could open up future treatment options for patients.: This paper highlights the crucial role of macrophage polarization in IBD. The authors shed light on the phenotype and function of macrophages and potential drug targets for polarization regulation. Existing approaches for regulating macrophage polarization are discussed and potential solutions for safety concerns are considered. We performed a literature search on the IBD and macrophage polarization mainly published in PubMed January 2010-July 2020.: Evidence indicates that there are fewer M2 macrophages and a high proportion of M1 macrophages in the intestinal tissues of individuals who are non- responsive to treatment. Regulating macrophage polarization is a potential novel targeted option for IBD treatment. Improved mechanistic insights are required to uncover more precise and effective targets for skewing macrophages into a proper phenotype.
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http://dx.doi.org/10.1080/14728222.2021.1901079DOI Listing
March 2021

Chronic active Epstein-Barr virus infection involving gastrointestinal tract mimicking inflammatory bowel disease.

BMC Gastroenterol 2020 Aug 5;20(1):257. Epub 2020 Aug 5.

Department of Gastroenterology, Huashan Hospital, Fudan University, Shanghai, China.

Background: Chronic active Epstein-Barr virus infection (CAEBV) is a rare disease, which is difficult to be differentiated from inflammatory bowel disease (IBD). To cause the attention, we present twelve cases of CAEBV in immunocompetent patients with gastrointestinal tract involvement.

Methods: Twelve patients who fulfilled the diagnostic criteria of CAEBV were enrolled in this retrospective study. The control group was consisted of twenty-four IBD patients with EBV-DNA value increased in peripheral blood. The clinicopathologic and endoscopic characteristics were reviewed and analyzed.

Results: The major clinical presentations of CAEBV patients were intermittent fever (100%), hepatomegaly/splenomegaly (58%), lymphadenopathy (50%), diarrhea (50%) and hematochezia (50%). Compared with IBD patients, the incidence of intermittent fever and increased level of ferritin were significantly higher among CAEBV patients. The median values for EBV detected in peripheral blood were significantly higher in CAEBV group (1.42*10^6 copies/μg) than in IBD group (3.2*10^3 copies/μg, p<0.05). The main endoscopic findings of CAEBV included multifocal or isolated, irregular, multiform ulcers and diffuse inflammation, lacking of typical cobblestone appearance. Ten patients died within 5 years of disease onset. The average survival time is 21 months.

Conclusions: Symptoms such as intermittent fever, increased level of ferritin and atypical endoscopic findings could be a sign for CAEBV. Early detections of EBV-DNA in serum and EBV-encoded small nuclear RNA (EBER) by in situ hybridization in intestinal tissue are essential for differential diagnosis between CAEBV and IBD.
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http://dx.doi.org/10.1186/s12876-020-01395-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7410156PMC
August 2020

Preparation of low-molecular-weight chondroitin sulfates by complex enzyme hydrolysis and their antioxidant activities.

Carbohydr Polym 2020 Aug 28;241:116302. Epub 2020 Apr 28.

College of Life Science, Northwestern Polytechnical University, Xian 710072, PR China; China Astronaut Research and Training Center, Beijing 100094, PR China. Electronic address:

Chondroitin sulfate (CS) has attracted widespread attention because of its numerous pharmacological activities. Low-molecular-weight chondroitin sulfates (LMWCSs) derived from the degradation of CS are reported to have better biological properties than whole CS. In this study, to obtain LMWCSs with high antioxidant activity, we depolymerized CS using complex enzymes, namely, chondroitinase ABC I (ChSase ABC I) and ChSase ABC II. The conditions of the complex enzyme hydrolysis (CEH) were optimized, and the structures and antioxidant activities of CS and LMWCSs were investigated. The results showed that the CEH conditions enhanced the antioxidant activities of the products as compared to CS. The basic structures of the LMWCSs and sulfate groups were well preserved after hydrolysis. Therefore, CEH provides an efficient and safe strategy to obtain LMWCSs, which can be used in antioxidant drugs, healthy foods, and cosmetics.
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http://dx.doi.org/10.1016/j.carbpol.2020.116302DOI Listing
August 2020

Computational screening of antagonists against the SARS-CoV-2 (COVID-19) coronavirus by molecular docking.

Int J Antimicrob Agents 2020 Aug 8;56(2):106012. Epub 2020 May 8.

SDIC Xinkai Water Environment Investment Co., Ltd, Tongzhou District, Beijing, 101101, China.

In the current spread of novel coronavirus (SARS-CoV-2), antiviral drug discovery is of great importance. AutoDock Vina was used to screen potential drugs by molecular docking with the structural protein and non-structural protein sites of new coronavirus. Ribavirin, a common antiviral drug, remdesivir, chloroquine and luteolin were studied. Honeysuckle is generally believed to have antiviral effects in traditional Chinese medicine. In this study, luteolin (the main flavonoid in honeysuckle) was found to bind with a high affinity to the same sites of the main protease of SARS-CoV-2 as the control molecule. Chloroquine has been proved clinically effective and can bind to the main protease; this may be the antiviral mechanism of this drug. The study was restricted to molecular docking without validation by molecular dynamics simulations. Interactions with the main protease may play a key role in fighting against viruses. Luteolin is a potential antiviral molecule worthy of attention.
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http://dx.doi.org/10.1016/j.ijantimicag.2020.106012DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7205718PMC
August 2020

Changing Trends of HIV, Syphilis, and Hepatitis C among Male Migrant Workers in Chongqing, China: Nine Consecutive Cross-Sectional Surveys, 2010-2018.

Int J Environ Res Public Health 2020 01 30;17(3). Epub 2020 Jan 30.

Department of Epidemiology and Health Statistics, School of Public Health and Management, Chongqing Medical University, Chongqing 400016, China.

Background: Male migrant workers (MMWs) have been reported to be vulnerable to human immunodeficiency virus (HIV) and other sexually transmitted infections (STIs). Chongqing, China is one of the major migration destinations and hotspots of HIV. This study aims to explore the prevalence of HIV, syphilis, and hepatitis C virus (HCV), as well as HIV-related knowledge and behaviors, among MMWs in Chongqing.

Methods: Questionnaire surveys were conducted, and blood samples were collected and examined among MMWs selected by two-stage stratified sampling in Chongqing from 2010 to 2018. The Cochran-Armitage trend test was conducted to observe the trends in the prevalence of HIV, syphilis, and HCV, as well as HIV-related knowledge and behaviors. The Chi-square test and Binary Logistic Regression were conducted to observe the distinctions between different groups.

Results: The overall HIV prevalence was 0.6% with an increasing trend (0.2% to 0.9%, < 0.001), whereas the overall HCV prevalence was 0.5% with a decreasing trend (0.5% to 0.4%, < 0.001). The overall syphilis prevalence was 1.3% in the ≥50 age group, 1.0% in the 30-49 age group, and higher than 0.3% in the 16-29 group (X = 19.527, P < 0.001). An uptrend (80.2%-80.6%, < 0.001) was observed in correct HIV-related knowledge. The 16-29 ((Odds Ratio) OR: 1.575; 95%CI (Confidence Interval): 1.380-1.798; P < 0.001) and 30-49 (OR: 1.697; 95%CI: 1.495-1.926; P < 0.001) age groups had 1.575 and 1.697 times correct HIV-related knowledge more than the ≥50 age group. The proportion of subjects engaged in commercial sex in the past year (7.7%-13.3%, < 0.001), consistent condom use during this activity (20.5%-54.0%, < 0.001), and condom use in the last commercial sex (48.6%-72.1%, = 0.020) were increasing. The risk of engaging in commercial sex in the past year in the 16-29 age group was 0.768 times (OR: 0.768; 95%CI: 0.643-0.917; P = 0.003) less than that in the ≥50 age group. The risk of engaging in non-regular sex in the past year in the 16-29 (OR: 2.819; 95%CI: 2.317-3.431; P < 0.001) and 30-49 (OR: 1.432; 95%CI: 1.184-1.733); P < 0.001) age groups were 2.819 and 1.432 times more than that in the ≥50 age group. The risk of engaging in anal sex in the past year in the 16-29 age group was 6.333 times (OR: 6.333; 95%CI: 1.468-27.327); P < 0.013] more than that in the ≥50 age group. The proportion of consistent condom use during non-regular sex in the past year (10.9%-47.3%, < 0.001) and condom use in the last non-regular sex (40.8%-71.1%, < 0.001) increased remarkably. The possibilities of consistent condom use during commercial sex in the past year in the 16-29 (OR: 2.606; 95%CI: 1.847-3.677); P < 0.001) and 30-49 (OR: 1.632; 95%CI: 1.214-2.195; P = 0.001) age groups were 2.606 and 1.632 times more than that in the ≥50 age group. The possibilities of condom use in the last commercial sex in the 16-29 (OR: 1.805; 95%CI: 1.258-2.589; P = 0.001) and 30-49 (OR: 1.360; 95%CI: 1.016-1.821; P = 0.039) age groups were 1.805 and 1.360 times more than that in the ≥50 age group. The possibilities of consistent condom use during non-regular sex in the past year (OR: 1.628; 95%CI: 1.066-2.484; P = 0.024) and condom use in the last non-regular sex (OR: 1.671; 95%CI: 1.148-2.433; P = 0.007) in the 16-29 age group were 1.628 and 1.671 times more than those in the ≥50 age group, respectively.

Conclusion: An upward trend of HIV and a downward trend of HCV were observed among MMWs in Chongqing from 2010 to 2018. We also found an increase in commercial sex and inadequate condom use during high-risk behaviors among this population. The overall syphilis prevalence in the middle-aged and elderly groups was higher than in the young group, and elderly MMWs were more likely to engage in unprotected high-risk behaviors. Thus, targeted STI prevention for MMWs in Chongqing, especially those aged 50 years and above, is urgently needed.
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http://dx.doi.org/10.3390/ijerph17030875DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7036957PMC
January 2020

Evaluation of Intravoxel Incoherent Motion Diffusion-Weighted Magnetic Resonance Imaging for Detection of Bowel Inflammation in Patients With Crohn Disease.

J Comput Assist Tomogr 2019 Sep/Oct;43(5):755-761

Department of Radiology, Jian'an District Centre Hospital (Fudan University, Huashan Hospital Jing'an Branch).

Objectives: This study aimed to evaluate the feasibility of intravoxel incoherent motion diffusion-weighted magnetic resonance imaging (DW-MRI) in detecting bowel inflammation in patients with Crohn disease (CD).

Methods: Sixteen patients who underwent intravoxel incoherent motion DW-MRI for CD and colonoscopy were recruited. Seventy-nine bowel segments were selected, and their mean D, D*, f, and apparent diffusion coefficient (ADC) values were measured. The receiver operating characteristic curve was performed to distinguish inflamed from normal bowel.

Results: The mean D, D*, f, and ADC values of inflamed bowel were significantly lower than those of normal bowel (P < 0.05). The area under the receiver operating characteristic curve for f (0.906) and ADC values (0.924) was greater than that for D (0.709) or D* values (0.686) for differentiating inflamed bowel from normal bowel (P < 0.05).

Conclusions: Intravoxel incoherent motion DW-MRI is a feasible technique for detecting inflammation in CD patients. The ADC and f values have more potential than the D and D* values.
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http://dx.doi.org/10.1097/RCT.0000000000000926DOI Listing
October 2019

The cardiovascular toxicity induced by high doses of gatifloxacin and ciprofloxacin in zebrafish.

Environ Pollut 2019 Nov 9;254(Pt B):112861. Epub 2019 Jul 9.

College of Bioengineering, Chongqing University, Chongqing 400044, PR China. Electronic address:

As a new type of pollutant, fluoroquinolones (FQs) antibiotics are ubiquitous in environment and have some threat to human health and ecological environment. Their ecological toxicity to the environment urgently need to be assessed. Therefore, we firstly explored the toxic effects and possible mechanism of cardiovascular toxicity induced by gatifloxacin (GTFX) and ciprofloxacin (CPFX) using zebrafish model. After 24 h exposure, the zebrafish treated with GTFX showed pericardial edema which was further investigated by histopathological examination, while CPFX exposure did not induce morphological abnormalities. However, both of them induced cardiac dysfunction, such as decreased heart rate and cardiac output which was showed a positive correlation with the concentration. To better understand the possible molecular mechanisms underlying cardiovascular toxicity in zebrafish, we investigated the transcriptional level of genes related to calcium signaling pathway and cardiac muscle contraction. The results indicated that the expression of ATPase (atp2a1l) and cardiac troponin C (tnnc1a) genes were significantly inhibited, the expression of calcium channel (cacna1ab) gene showed slight promoted trend after CPFX exposure. For zebrafish treated with GTFX, the expression of atp2a1l genes was also significantly inhibited, while the expression of tnnc1a genes was slightly inhibited and cacna1ab genes expression had no obvious effect. The present study firstly revealed that GTFX exposure can induce morphological and functional abnormalities on the cardiovascular system of zebrafish. Though CPFX exposure did not induce morphological abnormalities, the function of cardiovascular system was still damaged. Mechanistically, this toxicity might result from the pressure of down-regulation of genes associated with calcium signaling pathway and cardiac muscle contraction. The results of this study can provide a valuable theoretical basis for the establishment of FQs environmental quality standards in water environment, environmental drug regulation and risk management.
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http://dx.doi.org/10.1016/j.envpol.2019.07.029DOI Listing
November 2019

Residual flow may increase the risk of adverse events in patients received combined catheter ablation and transcatheter left atrial appendage closure for nonvalvular atrial fibrillation: a meta-analysis.

BMC Cardiovasc Disord 2019 06 10;19(1):138. Epub 2019 Jun 10.

Department of Cardiology, Nanjing University Medical School Affiliated Nanjing Drum Tower Hospital, Zhongshan Road No.321, Nanjing, 210000, China.

Background: Catheter ablation (CA) and left atrial appendage closure (LAAC) have been combined into a novel one-stop procedure for patients with atrial fibrillation (AF). However, postoperative complications are relatively common in patients undergoing LAAC; the complications, including residual flow, increase in the risk of bleeding, or other adverse events, are unknown in patients receiving one-stop therapy. Therefore, we tried to evaluate the adverse events of CA and LAAC hybrid therapy in patients with nonvalvular AF.

Methods: We performed a meta-analysis and computer-based literature search to identify publications listed in the PubMed, Embase, and Cochrane library databases. Studies were included if patients received CA and LAAC hybrid therapy and reported adverse events.

Results: Overall 13 studies involving 952 patients were eligible based on the inclusion criteria. In the periprocedural period, the pooled incidence of pericardial effusion was 3.15%. The rates of bleeding events and residual flow were 5.02 and 9.11%, respectively. During follow-up, the rates of all-cause mortality, embolism events, bleeding events, AF recurrence, and residual flow were 2.15, 5.24, 6.95, 32.89, and 15.35%, respectively. The maximum occurrence probability of residual flow events was 21.87%. Bleeding events were more common in patients with a higher procedural residual flow event rate (P = 0.03). A higher AF recurrence rate indicated higher rates of embolism events (P = 0.04) and residual flow (P = 0.03) during follow-up.

Conclusions: Bleeding events were more common in patients with a higher procedural residual flow event rate. However, combined CA and LAAC therapy is reasonably safe and efficacious in patients with nonvalvular AF. Further studies on the safety and efficacy of CA or LAAC alone are necessary in future.
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http://dx.doi.org/10.1186/s12872-019-1123-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6558863PMC
June 2019

Investigation of Perovskite Oxide SrCo Cu Nb O as a Cathode Material for Room Temperature Direct Ammonia Fuel Cells.

ChemSusChem 2019 Jun 22;12(12):2788-2794. Epub 2019 May 22.

School of Engineering, University of Warwick, Coventry, CV4 7AL, UK.

Single-phase perovskite oxide SrCo Cu Nb O was synthesized using a Pechini method. X-ray diffraction (XRD) analysis indicated a cubic structure with a=3.8806(7) Å. The oxide material was combined with active carbon, forming a composite electrode to be used as the cathode in a room temperature ammonia fuel cell based on an anion membrane electrolyte and NiCu/C anode. An open circuit voltage (OCV) of 0.19 V was observed with dilute 0.02 m (340 ppm) ammonia solution as the fuel. The power density and OCV were improved upon the addition of 1 m NaOH to the fuel, suggesting that the addition of NaOH, which could be achieved through the introduction of alkaline waste to the fuel stream, could improve performance when wastewater is used as the fuel. It was found that the SrCo Cu Nb O cathode was converted from irregular shape into shuttle-shape during the fuel cell measurements. As the key catalysts for electrode materials for this fuel cell are all inexpensive, after further development, this could be a promising technology for removal of ammonia from wastewater.
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http://dx.doi.org/10.1002/cssc.201900451DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6617732PMC
June 2019

The clinicopathologic features of chronic active Epstein-Barr virus infective enteritis.

Mod Pathol 2019 03 8;32(3):387-395. Epub 2018 Oct 8.

Center of Inflammatory Bowel Disease, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Zhejiang, China.

Chronic active Epstein-Barr virus infective enteritis (CAEBV enteritis) is rare and has not been well described yet. Therefore, we reported the clinicopathologic features of 11 patients with chronic active Epstein-Barr virus infective enteritis and their differences from inflammatory bowel disease. The major clinical presentations of chronic active Epstein-Barr virus infective enteritis were intermittent fever over 39 °C (100%), diarrhea (73%), abdominal pain (64%), lymphadenopathy (64%), splenomegaly (64%), and hepatomegaly (27%). The main endoscopic findings included numerous shallow, small, and irregular ulcers, mainly involving colon and small intestine together, no cobble-like appearance, and longitudinal ulcers. Compared to inflammatory bowel disease patients, the frequency of intermittent fever, hepatomegaly, splenomegaly, lymphadenopathy, the value of C-reactive protein, and serum Epstein-Barr virus DNA (EBV DNA) were significantly higher in chronic active Epstein-Barr virus infective enteritis patients (p < 0.01). The histologic findings show transmural inflammation with extended lymphoid infiltration, fissuring ulcers, and intraepithelial lymphocytosis. But chronic active Epstein-Barr virus infective enteritis lacked granulomas and connective tissue changes such as neural hypertrophy and thickened muscularis mucosae. Three chronic active Epstein-Barr virus infective enteritis patients died within 5 years of disease onset, and those three patients have received total colectomy, two of them died within 1 month after surgery. In this study, clinicopathologic features have been summarized to better recognize chronic active Epstein-Barr virus infective enteritis. There are resemblances between chronic active Epstein-Barr virus infective enteritis and inflammatory bowel disease, but some symptoms, signs, and indexes like intermittent fever, hepatomegaly, splenomegaly, lymphadenopathy, and elevated C-reactive protein, Epstein-Barr virus DNA are characteristics to differentiate chronic active Epstein-Barr virus infective enteritis and inflammation. Histopathological features also help the discrimination. Serum Epstein-Barr virus DNA and intestinal tissue in situ hybridization for Epstein-Barr virus-encoded RNA are recommended to exclude chronic active Epstein-Barr virus infective enteritis.
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http://dx.doi.org/10.1038/s41379-018-0144-1DOI Listing
March 2019

The impact of liposomal linolenic acid on gastrointestinal microbiota in mice.

Int J Nanomedicine 2018 9;13:1399-1409. Epub 2018 Mar 9.

Department of Digestive Diseases, Huashan Hospital Affiliated to Fudan University, Shanghai, China.

Background: The prevalence of has long been a global health issue. Triple therapy, being the first-line treatment, has caused dysbiosis of the gastrointestinal tract that led to various complications. A novel nanomedicine - liposomal linolenic acid (LipoLLA) - has been proven to have great potential in eradicating . However, the possible side effects of LipoLLA due to alteration of the gastrointestinal microbiota remain unknown.

Aim: This study focused on the impact of LipoLLA on gastrointestinal microbiota in mice in comparison with triple therapy in order to assess the safety profile.

Methods: Mice were divided into five groups: blank control group; control group; triple therapy group; low-dose LipoLLA group (25 mg/kg); and high-dose LipoLLA group (50 mg/kg). Fecal samples were collected before and after the intake of corresponding formulas. Gastric tissues were obtained after mice dissection. These samples were analyzed with high-throughput sequencing.

Results: The analysis revealed that LipoLLA resulted in minor gut microbiota alteration at different levels. The altered proportions in the high-dose group were higher than that of the low-dose group. On the other hand, the triple therapy group showed dramatic shifts in the major community composition. It displayed a notable boost in the relative abundance of and along with a decrease in that of and . All of them belonged to the major phyla in the microbiome. Triple therapy also led to the growth of the family , , and that may be associated with clinical illnesses. Gastric microbiota analysis reached similar conclusions.

Conclusion: Our findings indicated that LipoLLA causes minor gastrointestinal microbiota alterations while the triple therapy triggered dramatic changes. Thus, LipoLLA is not only promising but also a safe therapeutic medication to eradicate infection.
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http://dx.doi.org/10.2147/IJN.S151825DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5849918PMC
May 2018

Targeted Metagenome Based Analyses Show Gut Microbial Diversity of Inflammatory Bowel Disease patients.

Indian J Microbiol 2017 Sep 12;57(3):307-315. Epub 2017 May 12.

Department of Digestive Diseases, Huashan Hospital, Fudan University, 12 Middle Wulumuqi Rd., Shanghai, 200040 China.

Inflammatory bowel disease (IBD) is a multifactorial disease including both genetic and environmental factors. We compared the diversity of intestinal microbesamong a cohort of IBD patients to study the microbial ecological effects on IBD. Fecal samples from patients were sequenced with next generation sequence technology at 16S rDNA region. With statistical tools, microbial community was investigated at different level. The gut microbial diversity of Crohn's disease (CD) patients and colonic polyp (CP) patients significantly different from each other. However, the character of ulcerative colitis (UC) patients has of both CD and CP features. The microbial community from IBD patients can be very different (CD patient) or somewhat similar (UC patients) to non-IBD patients. Microbial diversity can be an important etiological factor for IBD clinical phenotype.
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http://dx.doi.org/10.1007/s12088-017-0652-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5574770PMC
September 2017

Expression of Glut-1 and HK-II in Pancreatic Cancer and Their Impact on Prognosis and FDG Accumulation.

Transl Oncol 2016 Dec;9(6):583-591

Department of Gastroenterology, Huashan Hospital, Fudan University, 12 Wulumuqi Middle Road, Shanghai 200040, China. Electronic address:

Objective: The purpose of this article is to analyze the expression of Glut-1 and HK-II, the association between their expression and F-FDG accumulation in pancreatic cancer.

Methods: Fifty patients with histologically proven pancreatic cancer were included in this preliminary study, all of whom received F-FDG PET/CT performance before surgery. Immunohistochemical staining of tumor tissue and adjacent normal tissue was performed for Glut-1 and HK-II. By combining proportions and intensity of immunochemical staining, we obtained the modified immunohistological scores for Glut-1 and HK-II respectively. The relationship between expression of Glut-1, HK-II and series of parameters was analyzed, i.e. clinicopathological characteristics, prognosis of patients and SUV of PET-CT.

Results: Compared with normal tissue, the Glut-1 and HK-II expression in pancreatic cancer tissue was significantly increased (P<.001). There was no correlation between expression of Glut-1, HK-II and age, gender, tumor size, tumor location, tumor histological type, tumor differentiation, the nerve infiltration, vascular invasion, local infiltration, lymph node metastasis or tumor staging in pancreatic cancer (P>.05). During the follow-up period, the survival curves of low Glut-1 group and high Glut-1 group were statistically different (P=.049). Multivariate analysis (Cox regression) revealed that Glut-1 expression was not associated with mortality (P>.05). No statistical difference was found in the survival curves of negative HK-II group and positive HK-II group (P=.545). There was no correlation between F-FDG uptake and expression of Glut-1 and HK-II(P>.05).

Conclusion: The Glut-1 and HK-II expression in pancreatic cancer tissue was significantly increased. There was no correlation between expression of Glut-1, HK-II and clinicopathological characteristics, prognosis and F-FDG uptake.
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http://dx.doi.org/10.1016/j.tranon.2016.08.004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5143352PMC
December 2016

A perovskite oxide with high conductivities in both air and reducing atmosphere for use as electrode for solid oxide fuel cells.

Sci Rep 2016 08 22;6:31839. Epub 2016 Aug 22.

School of Engineering, University of Warwick, Coventry CV4 7AL, UK.

Electrode materials which exhibit high conductivities in both oxidising and reducing atmospheres are in high demand for solid oxide fuel cells (SOFCs) and solid oxide electrolytic cells (SOECs). In this paper, we investigated Cu-doped SrFe0.9Nb0.1O3-δ finding that the primitive perovskite oxide SrFe0.8Cu0.1Nb0.1O3-δ (SFCN) exhibits a conductivity of 63 Scm(-1)and 60 Scm(-1) at 415 °C in air and 5%H2/Ar respectively. It is believed that the high conductivity in 5%H2/Ar is related to the exsolved Fe (or FeCu alloy) on exposure to a reducing atmosphere. To the best of our knowledge, the conductivity of SrFe0.8Cu0.1Nb0.1O3-δ in a reducing atmosphere is the highest of all reported oxides which also exhibit a high conductivity in air. Fuel cell performance using SrFe0.8Cu0.1Nb0.1O3-δ as the anode, (Y2O3)0.08(ZrO2)0.92 as the electrolyte and La0.8Sr0.2FeO3-δ as the cathode achieved a power density of 423 mWcm(-2) at 700 °C indicating that SFCN is a promising anode for SOFCs.
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http://dx.doi.org/10.1038/srep31839DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4992832PMC
August 2016

A simple high-performance matrix-free biomass molten carbonate fuel cell without CO2 recirculation.

Sci Adv 2016 08 17;2(8):e1600772. Epub 2016 Aug 17.

School of Engineering, University of Warwick, Coventry CV4 7AL, UK.; Department of Chemical Engineering, Monash University, Clayton, Melbourne, Victoria 3800, Australia.

In previous reports, flowing CO2 at the cathode is essential for either conventional molten carbonate fuel cells (MCFCs) based on molten carbonate/LiAlO2 electrolytes or matrix-free MCFCs. For the first time, we demonstrate a high-performance matrix-free MCFC without CO2 recirculation. At 800°C, power densities of 430 and 410 mW/cm(2) are achieved when biomass-bamboo charcoal and wood, respectively-is used as fuel. At 600°C, a stable performance is observed during the measured 90 hours after the initial degradation. In this MCFC, CO2 is produced at the anode when carbon-containing fuels are used. The produced CO2 then dissolves and diffuses to the cathode to react with oxygen in open air, forming the required [Formula: see text] or [Formula: see text] ions for continuous operation. The dissolved [Formula: see text] ions may also take part in the cell reactions. This provides a simple new fuel cell technology to directly convert carbon-containing fuels such as carbon and biomass into electricity with high efficiency.
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http://dx.doi.org/10.1126/sciadv.1600772DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4988772PMC
August 2016

Long-term ketogenic diet contributes to glycemic control but promotes lipid accumulation and hepatic steatosis in type 2 diabetic mice.

Nutr Res 2016 Apr 7;36(4):349-358. Epub 2015 Dec 7.

Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, Shanghai, China. Electronic address:

The ketogenic diet (KD) has been widely used in weight and glycemic control, although potential side effects of long-term KD treatment have caused persistent concern. In this study, we hypothesized that the KD would ameliorate the progression of diabetes but lead to disruptions in lipid metabolism and hepatic steatosis in a mouse model of diabetes. In type 2 diabetic mouse model, mice were fed a high-fat diet and administered streptozotocin treatment before given the test diets for 8 weeks. Subsequently, ameliorated glucose and insulin tolerance in KD-fed diabetic mice was found, although the body weight of high-fat diet- and KD-fed mice was similar. Interestingly, the weight of adipose tissue in KD mice was greater than in the other groups. The KD diet resulted in higher serum triacylglycerol and cholesterol levels in diabetic mice. Moreover, the KD-fed mice showed greater hepatic lipid accumulation. Mice fed the KD showed significant changes in several key genes such as sterol regulatory element-binding protein, fibroblast growth factor 21, and peroxisome proliferator-activated receptor α, which are all important in metabolism. In summary, KD ameliorates glucose and insulin tolerance in a mouse model of diabetes, but severe hepatic lipid accumulation and hepatic steatosis were observed, which should be considered carefully in the long-term application of KD.
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http://dx.doi.org/10.1016/j.nutres.2015.12.002DOI Listing
April 2016

Magnesium lithospermate B acts against dextran sodiumsulfate-induced ulcerative colitis by inhibiting activation of the NRLP3/ASC/Caspase-1 pathway.

Environ Toxicol Pharmacol 2016 Jan 10;41:72-7. Epub 2015 Nov 10.

Department of Gastroenterology, Huashan Hospital, Fudan University, Shanghai, China. Electronic address:

This study aimed to observe the therapeutic effects of magnesium lithospermate B on acute and chronic colitis induced by dextran sodiumsulfate (DSS) and the role of inflammasome complex (NOD-like receptor protein, NLRP; apoptosis-associated speck-like protein containing, ASC; caspase-1). Establishment of acute and chronic colitis models were by using 5% DSS oral administration in BALB/C male mice. Magnesium lithospermate B (240 mg/kg body weight) was given by subcutaneous injection. Samples were collected for biomarker assay, histological examination, immunohistochemical evaluation and western blot. There was obvious increase in TNF-α level and NLPR3, ASC, and caspase-1 expressions in acute and chronic colitis groups compared with the normal control. Significant decrease of the tumor necrosis factor-α level and the expressions of NLPR3, ASC, and caspase-1 were observed after treatment with magnesium lithospermate B. This study showed that magnesium lithospermate B could be used to treat acute and chronic colitis by inhibiting the activation of the NLRP3/ASC/Caspase-1 pathway.
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http://dx.doi.org/10.1016/j.etap.2015.10.009DOI Listing
January 2016

Identification of the hydroxylated derivatives of bufalin: phase I metabolites in rats.

J Asian Nat Prod Res 2016 17;18(3):239-47. Epub 2015 Nov 17.

d The Second Affiliated Hospital of Dalian Medical University , Dalian , 116044 , China.

Bufalin was a typical bioactive bufadienolide, existed in the traditional Chinese medicine Chan Su with the high content of 1-5%. The in vivo metabolites (1-5) of bufalin were prepared by various chromatographic techniques from the bile samples of SD rats, which were administrated with bufalin orally. Their structures were determined on the basis of the widely spectroscopic data, including HRESIMS, 1D-, and 2D NMR. And 1-3, 5 were new compounds. In the in vitro cytotoxicity assay, metabolites (1-5) showed weaker cytotoxic effects than bufalin against human cancer cell lines A549 and H1299, which indicated that the metabolism was a significant pathway for the detoxification of bufalin. Structures analyses indicated that metabolites 1-5 were hydroxylated derivatives of bufalin. This study suggested that Phase I metabolism catalyzed by CYP450 enzymes was one of the metabolic ways of bufalin, which may promote the excretion of bufalin.
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http://dx.doi.org/10.1080/10286020.2015.1071358DOI Listing
September 2016

Synthesis of ammonia directly from wet air using Sm(0.6)Ba(0.4)Fe(0.8)Cu(0.2)O(3-δ) as the catalyst.

Faraday Discuss 2015 ;182:353-63

Department of Chemical & Process Engineering, University of Strathclyde, Glasgow G1 1XJ, UK and School of Engineering, University of Warwick, Coventry, CV4 7AL, UK.

Ammonia was directly synthesised from wet air at 400 °C at atmospheric pressure. A new perovskite Sm(0.6)Ba(0.4)Fe(0.8)Cu(0.2)O(3-δ) was used as the electrocatalyst for electrochemical synthesis of ammonia. Ammonia formation rates of 9.19 × 10(-7) mol s(-1) m(-2) and 1.53 × 10(-6) mol s(-1) m(-2) were obtained at 400 °C when wet air and wet N2 were introduced into a simple single chamber reactor, respectively. The perovskite catalyst is low cost compared to the previously reported Ru/MgO and Pt/C catalysts. This experiment indicates that ammonia can be directly synthesised from wet air, a very promising simple technology for sustainable synthesis of ammonia in the future.
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http://dx.doi.org/10.1039/c5fd00033eDOI Listing
February 2016

A Light-Driven Therapy of Pancreatic Adenocarcinoma Using Gold Nanorods-Based Nanocarriers for Co-Delivery of Doxorubicin and siRNA.

Theranostics 2015 20;5(8):818-33. Epub 2015 Apr 20.

1. School of Electrical and Electronic Engineering, Nanyang Technological University, Singapore 639798, Singapore.

In this work, we report the engineering of polyelectrolyte polymers coated Gold nanorods (AuNRs)-based nanocarriers that are capable of co-delivering small interfering RNA (siRNA) and an anticancer drug doxorubicin (DOX) to Panc-1 cancer cells for combination of both chemo- and siRNA-mediated mutant K-Ras gene silencing therapy. Superior anticancer efficacy was observed through synergistic combination of promoted siRNA and DOX release upon irradiating the nanoplex formulation with 665 nm light. Our antitumor study shows that the synergistic effect of AuNRs nanoplex formulation with 665 nm light treatment is able to inhibit the in vivo tumor volume growth rate by 90%. The antitumor effect is contributed from the inactivation of K-Ras gene and thereby causing a profound synthesis (S) phase arrest in treated Panc-1 cells. Our study shows that the percentage of Panc-1 cells treated by nanoplex formulation with S phase is determined to be 35% and it is 17% much higher than that of Panc-1 cells without any treatments. The developed nanotherapy formulation here, that combines chemotherapy, RNA silencing and NIR window light-mediated therapy, will be seen to be the next natural step to be taken in the clinical research for improving the therapeutic outcomes of the pancreatic adenocarcinoma treatment.
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http://dx.doi.org/10.7150/thno.11335DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4440440PMC
February 2016

High ionic conductivity in a LiFeO2-LiAlO2 composite under H2/air fuel cell conditions.

Chemistry 2015 Jan 13;21(3):1350-8. Epub 2014 Nov 13.

Department of Chemical and Process Engineering, University of Strathclyde, Glasgow G1 1XJ (UK).

New ionic conducting materials for electrolytes for electrochemical devices have been attracting the interest of researchers in energy materials. Here, for the first time, we report a conductive composite with high ionic conductivity derived from an electronic conductor α-LiFeO2 and an insulator γ-LiAlO2. High conductivity was observed in the α-LiFeO2-γ-LiAlO2 composite when prepared by a solid state reaction method. However, the conductivity enhancement in α-LiFeO2-γ-LiAlO2 composite was not observed when the two oxides were mechanically mixed. The α-LiFeO2-γ-LiAlO2 composite also exhibits O(2-) or/and H(+) ionic conduction which was confirmed through H2/air fuel cell measurements. An exceptionally high conductivity of 0.50 S cm(-1) at 650 °C was observed under H2/air fuel cell conditions. This provides a new approach to discover novel ionic conductors from composite materials derived from electronic conductors.
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http://dx.doi.org/10.1002/chem.201404476DOI Listing
January 2015

Effectiveness of humor intervention for patients with schizophrenia: a randomized controlled trial.

J Psychiatr Res 2014 Dec 19;59:174-8. Epub 2014 Sep 19.

Wuhan City Mental Health Center, Wuhan, China.

Objective: The primary aim of this pilot study was to evaluate the possible therapeutic effects of a 10-session humor intervention program in improving rehabilitative outcomes and the effects of the intervention on patients' sense of humor among patients with schizophrenia.

Method: Thirty subjects were randomly assigned into either the intervention (humor skill training) group (n = 15) or the control (doing handwork) group (n = 15). The results were analyzed using descriptive statistics, t-tests and ANOVA.

Findings: Repeated measures analysis of variance (ANOVA) tests were conducted to examine the differences across conditions and time. A group by time interaction effect was observed on all of the outcomes, except positive symptoms of PANSS. The time main effect was also significant on the total score (p < 0.005) and the negative symptoms score (p < 0.001) of the PANSS.

Conclusions: The implementation of humor skill training in a mental health service can improve rehabilitative outcomes and sense of humor for schizophrenia patients who were in the rehabilitation stage.
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http://dx.doi.org/10.1016/j.jpsychires.2014.09.010DOI Listing
December 2014

Biotransformation of resibufogenin by Actinomucor elegans.

J Asian Nat Prod Res 2014 9;16(6):623-8. Epub 2014 Jun 9.

a School of Traditional Chinese Medicines, Shenyang Pharmaceutical University , Shenyang 110016 , China.

Resibufogenin (RB), a major bioactive bufadienolide, has the potential anticancer activity. In the present work, biotransformation of RB by Actinomucor elegans AS 3.2778 yielded five products, namely 3-oxo-resibufogenin (1), 3-epi-resibufogenin (2), 3-epi-12-oxo-hydroxylresibufogenin (3), 3α-acetoxy-15α-hydroxylbufalin (4), and 3-epi-12α-hydroxylresibufogenin (5), respectively. Among them, metabolites 3 and 4 are previously unreported. The chemical structures of metabolites 1-5 were fully elucidated on the basis of 2D NMR and HR-MS. The highly stereo- and regio-specific isomerization, hydroxylation, and esterification reactions were observed in the biotransformation process of RB by A. elegans. Their cytotoxicities against A549 and H1299 cells were evaluated.
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http://dx.doi.org/10.1080/10286020.2014.921911DOI Listing
September 2014

Microbial transformation of resibufogenin by Curvularia lunata AS 3.4381.

J Asian Nat Prod Res 2014 ;16(3):290-5

a College of Bioengineering, Beijing Polytechnic , Beijing , 100029 , China.

In this paper, the microbial transformation of resibufogenin by Curvularia lunata AS 3.4381 was investigated, and four transformed products were isolated and characterized as 3-epi-resibufogenin (2), 12α-hydroxy-3-epi-resibufogenin (3), 12-oxo-16β-hydroxy-3-epi-resibufogenin (4), and 12β,15-epoxy-3-epi-bufalin-14,15-ene (5). Among them, 4 and 5 are new compounds, and isomerization, hydroxylation, and oxidation reactions in microbial transformation process were observed. Additionally, the cytotoxicities of transformed products (2-5) were also investigated.
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http://dx.doi.org/10.1080/10286020.2013.878332DOI Listing
March 2014

Isolation of cyanidin 3-glucoside from blue honeysuckle fruits by high-speed counter-current chromatography.

Food Chem 2014 22;152:386-90. Epub 2013 Nov 22.

College of Bioengineering, Beijing Polytechnic, No.1 Shaoyaoju, Beijing 100029, China.

Blue honeysuckle fruits are rich in anthocyanins with many beneficial effects such as reduction of the risk of cardiovascular diseases, diabetes and cancers. High-speed counter-current chromatography (HSCCC) was used for the separation of anthocyanin on a preparative scale from blue honeysuckle fruit crude extract with a biphasic solvent system composed of tert-butyl methyl ether/n-butanol/acetonitrile/water/trifluoroacetic acid (2:2:1:5:0.01, v/v) for the first time in this paper. Each injection of 100 mg crude extract yielded 22.8 mg of cyanidin 3-glucoside (C3G) at 98.1% purity. The compound was identified by means of electro-spray ionisation mass (ESI/MS) and (1)H and (13)C nuclear magnetic resonance (NMR) spectra.
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http://dx.doi.org/10.1016/j.foodchem.2013.11.080DOI Listing
September 2014

Preparation of silver nanoparticles by a non-aqueous sol-gel process.

J Nanosci Nanotechnol 2013 Aug;13(8):5445-51

Department of Chemical and Process Engineering, University of Strathclyde, Glasgow G1 1XJ, UK.

Using a non-aqueous sol-gel process with a direct calcination step in air after prior drying, silver nanoparticles with average size distribution ranging from 20 to 100 nm were synthesised. Studies in reduced atmosphere were also performed with mixed results, both in phase and particle size, as the samples were found to be mixed with an amorphous phase. In oxidising atmosphere, the temperature and dwelling time were found to be critical factors with the former playing a larger role than the latter. Optimally nanoparticles of silver are best prepared by direct calcination in air of the precursor gel at 250 degrees C for 1 hour. Compared to silver particles prepared by microemulsions, the particle size is larger due to the thermal treatment, which causes a growth of the silver particles.
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http://dx.doi.org/10.1166/jnn.2013.7446DOI Listing
August 2013

Biotransformation of osthole by Alternaria longipes.

J Asian Nat Prod Res 2013 Jul 16;15(7):717-22. Epub 2013 May 16.

College of Bioengineering, Beijing Polytechnic, China.

The biotransformation of osthole (1) by Alternaria longipes was carried out, and five transformed products were obtained in the present research work. Based on their extensive spectral data, the structures of these metabolites were characterized as 4'-hydroxyl-osthole (2), 4'-hydroxyl-2',3'-dihydroosthole (3), 2',3'-dihydroxylosthole (4), osthole-4'-oic acid methyl ester (5), and osthole-4'-oic acid glucuron-1-yl ester (6), respectively. Among them, products 5 and 6 were new compounds.
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http://dx.doi.org/10.1080/10286020.2013.795951DOI Listing
July 2013

Protective effect of magnesium lithospermate B against dextran sodiumsulfate induced ulcerative colitis in mice.

Environ Toxicol Pharmacol 2013 Jul 22;36(1):97-102. Epub 2013 Mar 22.

Department of Gastroenterology, Huashan Hospital, Fudan University, China.

Anti-platelet drugs have been used to treat inflammatory bowel disease. In this study, we observed the therapeutic effects of magnesium lithospermate B, a main component of salvianolate, on colitis induced by dextran sodiumsulfate (DSS). Colitis was induced by 5% DSS oral administration in BALB/C male mice. Magnesium lithospermate B (60-240mg/kg) was given by subcutaneous injection for 2 weeks. Then, mice were sacrificed; serum and colon tissues were collected for biomarker assay, histological examination, immunohistochemical study and real-time quantitative polymerase chain reaction. DSS induced gross bleeding, inflammation, crypt damage and mucosal damage in colon. Treatment with magnesium lithospermate B could reduce colon inflammation induced by DSS. Magnesium lithospermate B could reverse the high CD40/CD40L expression and hypercoagulable state induced by DSS in colon. This study showed that magnesium lithospermate B could be used to treat colitis. The protective effects of magnesium lithospermate B may be due to its effects on CD40/CD40L expression and blood clotting status.
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http://dx.doi.org/10.1016/j.etap.2013.03.010DOI Listing
July 2013
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