Publications by authors named "L V Smagliy"

9 Publications

Role of HS in Regulation of Vascular Tone in Metabolic Disorders.

Bull Exp Biol Med 2021 Aug 20;171(4):431-434. Epub 2021 Sep 20.

Siberian State Medical University, Ministry of Health of the Russian Federation, Tomsk, Russia.

We studied the effect of the HS donor (NaHS, 1-500 μM) on the contractile responses of isolated aortic smooth muscle segments from rats with metabolic syndrome induced by high-fat, high-carbohydrate diet. It was found that the vasorelaxing effect of NaHS (5-100 μM) decreased in under conditions of MS. The endothelial NO synthase inhibitor L-NAME (100 μM) suppressed the effect of NaHS, while cystathionine-gamma-lyase inhibitor PAG (100 μM) decreased the vasodilating effects of acetylcholine (0.1-100 μM). Application of endogenous NO precursor L-arginine (1 mM) potentiated in the effects of HS donor NaHS. Thus, the contractile activity of vascular smooth muscles in metabolic syndrome is determined by not only the effect of HS, but also the influence of NO.
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http://dx.doi.org/10.1007/s10517-021-05243-yDOI Listing
August 2021

Regulation of Vascular Smooth Muscle Contractions in the Model of Metabolic Syndrome.

Bull Exp Biol Med 2020 Dec 2;170(2):196-199. Epub 2020 Dec 2.

Siberian State Medical University, Ministry of Health of the Russian Federation, Tomsk, Russia.

Reduced glucose tolerance, hyperglycemia, and imbalance in lipid levels were found in rats with metabolic syndrome induced by a high-fat, high-carbohydrate diet. The contractile responses of intact and endothelium-denuded aortic smooth muscle segments from rats with metabolic syndrome to application of acetylcholine, phenylephrine, sodium nitroprusside, and forskolin were studied by mechanographic method. It was found that endothelial dysfunction develops against the background of metabolic and hemodynamic disorders in metabolic syndrome. It was shown that the regulation of vasoconstrictor reactions of vascular smooth muscles in metabolic syndrome is due to a decrease in Ca entry, mainly voltage-independent, as well as changes in the function of cGMP- and cAMP-activated K-channels.
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http://dx.doi.org/10.1007/s10517-020-05031-0DOI Listing
December 2020

HS-Mediated Changes in Erythrocyte Volume: Role of Gardos Channels, Na,K,2Cl Cotransport and Anion Exchanger.

Bull Exp Biol Med 2019 Aug 7;167(4):508-511. Epub 2019 Sep 7.

Siberian State Medical University, Ministry of Health of the Russian Federation, Tomsk, Russia.

The effect of HS on changes in erythrocyte volume was studied by spectrophotometrical and potentiometric methods. It was found that HS donor NaHS (2.5, 10, and 100 μM) induced an increase in erythrocyte volume in heterosmotic media. Activation of Gardos channels with A23187 or ascorbate-phenazine methosulfate system causes erythrocyte shrinkage and hyperpolarization of their membrane, while addition of NaHS restored erythrocyte volume. The decrease in erythrocyte volume upon blockade of Na,K,2Cl cotransporter (bumetanide) or anion exchanger (SITS) was abolished by HS donor NaHS, which attested to an important role of these transporters and chlorine conductivity of the membrane in the maintenance of the homeostasis of blood cells.
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http://dx.doi.org/10.1007/s10517-019-04561-6DOI Listing
August 2019

Role of Carbon Monoxide in the Mechanisms of Action of Extracellular ATP on Contractile Activity of Vascular Smooth Muscle Cells.

Bull Exp Biol Med 2019 Jul 26;167(3):363-366. Epub 2019 Jul 26.

Siberian State Medical University, Ministry of Health of the Russian Federation, Tomsk, Russia.

We studied the role of carbon monoxide (CO) in the effect of P2X and P2Y receptor agonist ATP on the tone of rat aorta segments with intact endothelium. ATP (1-1000 μM) and P2X receptor agonist α,β-MeATP (100 μM) relaxed segments precontracted with phenylephrine (10 μM), while UTP (100-1000 μM) increased the amplitude of phenylephrine-induced contraction. The relaxing effect of ATP was enhanced by CORM II (100 μM), NO synthase inhibitor L-NAME, and guanylate cyclase inhibitor ODQ and attenuated by ZnPP IX (100 μM). The constrictive effect of UTP was weakened by CORM II (100 μM), but was not changed by ZnPP IX (100 μM). ZnPP IX (100 μM) weakened the relaxation response to α,β-MeATP. Thus, ATP involves the CO-dependent signaling cascade through P2X receptors.
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http://dx.doi.org/10.1007/s10517-019-04527-8DOI Listing
July 2019

Molecular Mechanisms of Action of Gas Transmitters NO, CO and H2S in Smooth Muscle Cells and Effect of NO-generating Compounds (Nitrates and Nitrites) on Average Life Expectancy.

Usp Fiziol Nauk 2017 Jan-Mar;48(1):24-52

Gaseous signaling molecules (gas transmitters) take an especial position among the numerous signaling molecules involved in the regulation of both intracellular processes that occur in different types of cells and cell-cell interactions. At present time, gas transmitters include three molecules whose enzymatic systems of synthesis and degradation, physiological action and intracellular effectors, the change of which under the action of gas transmitters may result in physiological and/or pathophysiological effects are well- determined. These molecules include nitrogen oxide (NO), carbon monoxide (CO) and hydrogen sulfide (H2S). They are involved in the regulation of functions of various organs and systems of the human body, including the circulatory system. Interaction of NO, CO and H2S with various enzymatic and structural components of endothelial and, especially, smooth muscle cells has a significant impact on vascular tone and blood pressure. Furthermore, the crossing of NO-, CO- and H2S-mediated signaling pathways at common effectors and interaction with each other can determine the end, resulting functional response of the cell. The knowledge of the molecular targets of gas transmitters' action, the structure of the binding centers for gas transmitters and their interaction with each other may be essential in the development of methods of regulation of these signaling systems by targeted, directed action. This review summarizes the molecular mechanisms of the NO, CO and H2S interaction with the main targets, which carry out their regulatory effect on vascular smooth muscle cells. Also we describe here different ways of cross-regulation of NO-, CO- and H2S-dependent signaling pathways. We analyzed NO-synthase and nitrite reductase systems of nitric oxide cycle and discuss the nitrate-nitrite background of the existence of modern man, which can substantially modify the signaling system, the metabolism of virtually all cell ultrastructure of neurons, neuron-neuron and neuron-glial interactions and exerts its influence on socially significant diseases that can affect the quality and the average life expectancy.
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January 2018
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