Publications by authors named "L Cetina-Montejo"

3 Publications

  • Page 1 of 1

evaluation of anthraquinones from ( Miller) roots and several derivatives against strains of influenza virus.

Ind Crops Prod 2019 Jun 7;132:468-475. Epub 2019 Mar 7.

Unidad de Biotecnología, Centro de Investigación Científica de Yucatán, Calle 43 Número 130 × 32 y 34, CP 97205, Mérida, Yucatán, Mexico.

is a crop of wide economic value of worldwide distribution, and a rich source of quinone components. Recently, antiviral aloe anthraquinones had been reported against human influenza virus. In the present work two anthraquinones, aloesaponarin-I () and aloesaponarin-II () were isolated from roots, and six derivatives were obtained by methylation (), acetylation () and -glycosyl () reactions starting from (). Additionally, a new Tetra--acetyl-β-d-glucopyranosyl derivative from was also prepared. All compounds were evaluated against two strains of influenza virus AH1N1 by cytopathic effect reduction assay (CPE). The antiviral activity was determined by the ability of compounds to inhibit virus replication on Madin Darby Canine Kidney cells (MDCK). New derivatives 3-(2´,3´,4´,6´-Tetra-O-acetyl-β-d-glucopyranosyl-aloesaponarin-I () and -(2´,3´,4´,6´-Tetra-O-acetyl-β-d-glucopyranosyl- aloesaponarin-II () showed a cytopathic reduction effect against influenza strain A/Yucatán/2370/09 with IC of 30.77 and 13.70 μM, and against the virus A/Mexico/InDRE797/10 with IC of 62.28 and 19.47 μM, respectively. To assess the effect of derivatives and during one cycle of replication (0-10 h), a time-of-addition experiment was performed. As a result it was found that both compounds were most effective when added 6-10 h post-infection and significantly inhibited viral titre (> 70%) at the concentrations of 50 and 100 μM. Based on the structural analysis of the compounds, it was suggested that the Tetra-O-acetyl-β-d-glucopyranosyl substituent at the C3 position of the anthraquinone might have an effect against the influenza AH1N1 virus.
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http://dx.doi.org/10.1016/j.indcrop.2019.02.056DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7138392PMC
June 2019

Zeylanone epoxide isolated from Diospyros anisandra stem bark inhibits influenza virus in vitro.

Arch Virol 2019 Jun 23;164(6):1543-1552. Epub 2019 Mar 23.

Unidad de Biotecnología, Centro de Investigación Científica de Yucatán, Calle 43 Número 130 x 32 y 34, CP 97205, Mérida, Yucatán, México.

Influenza virus infection is a public health problem, causing significant morbidity and mortality. Currently, zanamivir and oseltamivir are in common use, and there are already reports of antiviral resistance. Several studies have shown the antiviral potential of a wide variety of plant-based natural compounds, among them those of the quinone type. In this study, we evaluated the antiviral activity of naphthoquinones isolated from the stem bark of Diospyros anisandra, and we selected zeylanone epoxide (ZEP) to study its effects on influenza A and B viruses. Our results indicated that ZEP inhibits the replication of influenza A and B viruses, at early and middle stages of the replication cycle. Confined nuclear localization of the viral NP indicated that ZEP affects its intracellular distribution and reduces viral yield. This is the first report on the antiviral properties and possible mechanism of action of ZEP in vitro, showing its broad-spectrum activity against influenza A and B viruses.
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http://dx.doi.org/10.1007/s00705-019-04223-yDOI Listing
June 2019

Hemagglutinin variants of influenza A(H1N1)pdm09 virus with reduced affinity for sialic acid receptors.

Arch Virol 2014 May 3;159(5):1207-11. Epub 2013 Dec 3.

Centro de Investigaciones Regionales Dr. Hideyo Noguchi, Universidad Autonoma de Yucatan, Av. Itzaes #490 x 59, Centro, C. P. 97000, Merida, Yucatan, Mexico,

In influenza A H1 virus, amino acids at position 190 and 225 of HA affect replication and transmission. In this study, we show that the mutation D190Y in the HA of influenza AH1N1pdm09 virus reduces the affinity of the virus for sialic acid receptors expressed at the surface of red blood cells from different species without affecting virus replication in MDCK cells.
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http://dx.doi.org/10.1007/s00705-013-1934-xDOI Listing
May 2014