Dr. Kyunggon Kim, PhD - Asan Medical Center, University of Ulsan, College of Medicine - Assistant Professor

Dr. Kyunggon Kim

PhD

Asan Medical Center, University of Ulsan, College of Medicine

Assistant Professor

Seoul, Songpa | South Korea

Main Specialties: Other

Additional Specialties: Clinical proteomics

ORCID logohttps://orcid.org/0000-0002-3072-5331

Dr. Kyunggon Kim, PhD - Asan Medical Center, University of Ulsan, College of Medicine - Assistant Professor

Dr. Kyunggon Kim

PhD

Introduction

Primary Affiliation: Asan Medical Center, University of Ulsan, College of Medicine - Seoul, Songpa , South Korea

Specialties:

Additional Specialties:

Research Interests:

Education

Apr 2013
Northwestern University
Post doctoral
Top down proteomics
Sep 2006
Seoul National University
PhD
Proteomics (Mass spectrometry)
Mar 2004
Seoul National University
Master
Structural Biology (Protein X-ray crystallography)
Mar 1996
Yonsei University
Bachelor
Biotechnology

Publications

44Publications

730Reads

3Profile Views

299PubMed Central Citations

Plasma-based protein biomarkers can predict the risk of acute graft-versus-host disease and non-relapse mortality in patients undergoing allogeneic hematopoietic stem cell transplantation.

Blood Cells Mol Dis 2019 02 4;74:5-12. Epub 2018 Oct 4.

Department of Internal Medicine, Seoul National University Hospital, Seoul, Republic of Korea; Cancer Research Institute, Seoul National University College of Medicine, Seoul, Republic of Korea. Electronic address:

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https://linkinghub.elsevier.com/retrieve/pii/S10799796183030
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http://dx.doi.org/10.1016/j.bcmd.2018.10.001DOI Listing
February 2019
20 Reads
2.646 Impact Factor

NEDD4L limits cAMP signaling through ubiquitination of CREB-regulated transcription coactivator 3.

FASEB J 2018 07 5;32(7):4053-4062. Epub 2018 Mar 5.

Department of Biomedical Sciences, Asan Medical Center, Asan Institute for Life Sciences, University of Ulsan College of Medicine, Seoul, South Korea.

The transcription factor cAMP-responsive element-binding protein (CREB) is involved in a variety of physiologic processes. Although its activity appears to be largely correlated with its phosphorylation status, cAMP-mediated dephosphorylation and the subsequent nuclear migration of the CREB-regulated transcription factors (CRTCs) are required to stimulate CREB transcriptional activity. Among the 3 identified mammalian homologs of CRTCs, CRTC3 has been shown to be expressed predominantly in adipose tissues in response to catecholamine signals that regulate lipid metabolism. Here, we show that prolonged cAMP signaling down-regulates CRTC3 in a proteasome-dependent manner and that neural precursor cell-expressed developmentally down-regulated gene 4-like (NEDD4L), a specific ubiquitin ligase for CRTC3, is responsible for this process. By recognizing the PY motif of CRTC3, NEDD4L interacts with CRTC3 and promotes its polyubiquitination. Interaction between NEDD4L and CRTC3 is further boosted by cAMP signaling, and this enhanced interaction appears to be dependent on the cAMP-mediated phosphorylation of NEDD4L at the Ser448 site. Furthermore, we show that food withdrawal stimulates NEDD4L phosphorylation in mice, which then show a decrease of adipose tissue CRTC3 protein levels. Together, these results suggest that NEDD4L plays a key role in the feedback regulation of cAMP signaling by limiting CRTC3 protein levels.-Kim, Y.-H., Yoo, H., Hong, A.-R., Kwon, M., Kang, S.-W., Kim, K., Song, Y. NEDD4L limits cAMP signaling through ubiquitination of CREB-regulated transcription coactivator 3.

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http://dx.doi.org/10.1096/fj.201701406RDOI Listing
July 2018
3 Reads
5.043 Impact Factor

Tumour growth rate of follicular thyroid carcinoma is not different from that of follicular adenoma.

Clin Endocrinol (Oxf) 2018 06 2;88(6):936-942. Epub 2018 Apr 2.

Departments of Internal Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.

Objective: Distinguishing malignancy from benign thyroid nodule has always been challenging, especially in follicular lesions. Thyroid nodules with small size and indeterminate cytology do not lead to immediate surgery. We tried to evaluate whether tumour size and tumour growth rate can distinguish follicular thyroid carcinoma (FTC) from follicular adenoma (FA).

Design And Patients: This retrospective study included patients with pathologically proven FTCs (n = 50) and FAs (n = 110) who underwent preoperative serial neck ultrasonography (US) at least 3 times: it comprises 30% of all follicular tumours (32% FAs and 25% FTCs). The growth rates of follicular tumours on serial US were measured using at least 3 consecutive examinations during a median follow-up of 4.1 years (range, 0.7-13.3 years) by experienced radiologists.

Results: The FA and FTC groups showed no significant difference in clinicopathological characteristics, including age, proportion of large nodules (>4 cm) and preoperative cytology. The maximum diameter of thyroid nodule was gradually increased in both groups with statistical significance (P < .001 and P < .001, respectively). No significant differences in change of maximum diameter of thyroid nodule (P = .132) and tumour volume (P = .208) were found between the FA and FTC groups during the follow-up. The median time to a significant tumour growth from baseline was not different between the FA and FTC groups (1.4 years and 1.7 years, respectively, P = .556). When we divided the patients into four groups (rapid, moderate, slow and no growth) according to the growth velocity of the thyroid tumours, no significant difference in growth velocity was found among the groups.

Conclusions: The tumour size and growth rate of the thyroid nodule itself could not predict malignancy. Diagnostic approaches that use molecular markers would be more important than clinical features for the decision of diagnostic surgery for patients with follicular tumours.

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http://dx.doi.org/10.1111/cen.13591DOI Listing
June 2018
7 Reads
3.457 Impact Factor

BRAF and RAS Mutational Status in Noninvasive Follicular Thyroid Neoplasm with Papillary-Like Nuclear Features and Invasive Subtype of Encapsulated Follicular Variant of Papillary Thyroid Carcinoma in Korea.

Thyroid 2018 04 16;28(4):504-510. Epub 2018 Mar 16.

3 Department of Pathology, Asan Medical Center, University of Ulsan College of Medicine , Seoul, Korea.

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http://dx.doi.org/10.1089/thy.2017.0382DOI Listing
April 2018
19 Reads
4.493 Impact Factor

Top-Down Proteomics Enables Comparative Analysis of Brain Proteoforms Between Mouse Strains.

Anal Chem 2018 03 26;90(6):3802-3810. Epub 2018 Feb 26.

Departments of Chemistry, Molecular Biosciences and the Proteomics Center of Excellence , Northwestern University , 2145 North Sheridan Road , Evanston , Illinois 60208 , United States.

Over the past decade, advances in mass spectrometry-based proteomics have accelerated brain proteome research aimed at studying the expression, dynamic modification, interaction and function of proteins in the nervous system that are associated with physiological and behavioral processes. With the latest hardware and software improvements in top-down mass spectrometry, the technology has expanded from mere protein profiling to high-throughput identification and quantification of intact proteoforms. Murine systems are broadly used as models to study human diseases. Neuroscientists specifically study the mouse brain from inbred strains to help understand how strain-specific genotype and phenotype affect development, functioning, and disease progression. This work describes the first application of label-free quantitative top-down proteomics to the analysis of the mouse brain proteome. Operating in discovery mode, we determined physiochemical differences in brain tissue from four healthy inbred strains, C57BL/6J, DBA/2J, FVB/NJ, and BALB/cByJ, after probing their intact proteome in the 3.5-30 kDa mass range. We also disseminate these findings using a new tool for top-down proteomics, TDViewer and cataloged them in a newly established Mouse Brain Proteoform Atlas. The analysis of brain tissues from the four strains identified 131 gene products leading to the full characterization of 343 of the 593 proteoforms identified. Within the results, singly and doubly phosphorylated ARPP-21 proteoforms, known to inhibit calmodulin, were differentially expressed across the four strains. Gene ontology (GO) analysis for detected differentially expressed proteoforms also helps to illuminate the similarities and dissimilarities in phenotypes among these inbred strains.

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http://dx.doi.org/10.1021/acs.analchem.7b04108DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5861018PMC
March 2018
36 Reads
6.042 Impact Factor

Proteoforms in Peripheral Blood Mononuclear Cells as Novel Rejection Biomarkers in Liver Transplant Recipients.

Am J Transplant 2017 Sep 27;17(9):2458-2467. Epub 2017 Jun 27.

Comprehensive Transplant Center, Northwestern University Feinberg School of Medicine, Chicago, IL.

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http://dx.doi.org/10.1111/ajt.14359DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5612406PMC
September 2017
36 Reads
3 Citations
5.683 Impact Factor

Sirt1 Regulates DNA Methylation and Differentiation Potential of Embryonic Stem Cells by Antagonizing Dnmt3l.

Cell Rep 2017 02;18(8):1930-1945

Department of Biomedical Sciences, Asan Medical Center, University of Ulsan College of Medicine, Seoul 05505, Korea; Department of Physiology, University of Ulsan College of Medicine, Seoul 05505, Korea. Electronic address:

Embryonic stem cell (ESC) abnormalities in genome methylation hamper the utility of their therapeutic derivatives; however, the underlying mechanisms are unknown. Here, we show that the nicotinamide adenine dinucleotide (NAD)-dependent deacetylase, Sirt1, selectively prevents abnormal DNA methylation of some developmental genes in murine ESCs by antagonizing Dnmt3l. Transcriptome and DNA methylome analyses demonstrated that Sirt1-null (Sirt1) ESCs repress expression of a subset of imprinted and germline genes concomitant with increased DNA methylation of regulatory elements. Dnmt3l was highly expressed in Sirt1 ESCs, and knockdown partially rescued abnormal DNA methylation of the Sirt1 target genes. The Sirt1 protein suppressed transcription of Dnmt3l and physically interacted with the Dnmt3l protein, deacetylating and destabilizing Dnmt3l protein. Sirt1 deficiency delayed neurogenesis and spermatogenesis. These differentiation delays were significantly or partially abolished by reintroduction of Sirt1 cDNA or Dnmt3l knockdown. This study sheds light on mechanisms that restrain DNA methylation of developmentally vital genes operating in ESCs.

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http://dx.doi.org/10.1016/j.celrep.2017.01.074DOI Listing
February 2017
44 Reads
8.032 Impact Factor

4E-BP is a target of the GCN2-ATF4 pathway during Drosophila development and aging.

J Cell Biol 2017 01 15;216(1):115-129. Epub 2016 Dec 15.

Department of Cell Biology, New York University School of Medicine, New York, NY 10016

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http://dx.doi.org/10.1083/jcb.201511073DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5223598PMC
January 2017
24 Reads
7 Citations
9.834 Impact Factor

Optical Aptamer Probes of Fluorescent Imaging to Rapid Monitoring of Circulating Tumor Cell.

Sensors (Basel) 2016 Nov 23;16(11). Epub 2016 Nov 23.

The Institute for the 3Rs, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Korea.

Fluorescence detecting of exogenous EpCAM (epithelial cell adhesion molecule) or muc1 (mucin1) expression correlated to cancer metastasis using nanoparticles provides pivotal information on CTC (circulating tumor cell) occurrence in a noninvasive tool. In this study, we study a new skill to detect extracellular EpCAM/muc1 using quantum dot-based aptamer beacon (QD-EpCAM/muc1 ALB (aptamer linker beacon). The QD-EpCAM/muc1 ALB was designed using QDs (quantum dots) and probe. The EpCAM/muc1-targeting aptamer contains a Ep-CAM/muc1 binding sequence and BHQ1 (black hole quencher 1) or BHQ2 (black hole quencher2). In the absence of target EpCAM/muc1, the QD-EpCAM/muc1 ALB forms a partial duplex loop-like aptamer beacon and remained in quenched state because the BHQ1/2 quenches the fluorescence signal-on of the QD-EpCAM/muc1 ALB. The binding of EpCAM/muc1 of CTC to the EpCAM/muc1 binding aptamer sequence of the EpCAM/muc1-targeting oligonucleotide triggered the dissociation of the BHQ1/2 quencher and subsequent signal-on of a green/red fluorescence signal. Furthermore, acute inflammation was stimulated by trigger such as caerulein in vivo, which resulted in increased fluorescent signal of the cy5.5-EpCAM/muc1 ALB during cancer metastasis due to exogenous expression of EpCAM/muc1 in Panc02-implanted mouse model.

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http://dx.doi.org/10.3390/s16111909DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5134568PMC
November 2016
6 Reads
2.677 Impact Factor

Development of Diagnostic Biomarkers for Detecting Diabetic Retinopathy at Early Stages Using Quantitative Proteomics.

J Diabetes Res 2016 9;2016:6571976. Epub 2015 Nov 9.

Department of Biomedical Sciences, Seoul National University College of Medicine, 28 Yongon-Dong, Seoul 110-799, Republic of Korea ; Department of Biomedical Engineering, Seoul National University College of Medicine, 28 Yongon-Dong, Seoul 110-799, Republic of Korea.

Diabetic retinopathy (DR) is a common microvascular complication caused by diabetes mellitus (DM) and is a leading cause of vision impairment and loss among adults. Here, we performed a comprehensive proteomic analysis to discover biomarkers for DR. First, to identify biomarker candidates that are specifically expressed in human vitreous, we performed data-mining on both previously published DR-related studies and our experimental data; 96 proteins were then selected. To confirm and validate the selected biomarker candidates, candidates were selected, confirmed, and validated using plasma from diabetic patients without DR (No DR) and diabetics with mild or moderate nonproliferative diabetic retinopathy (Mi or Mo NPDR) using semiquantitative multiple reaction monitoring (SQ-MRM) and stable-isotope dilution multiple reaction monitoring (SID-MRM). Additionally, we performed a multiplex assay using 15 biomarker candidates identified in the SID-MRM analysis, which resulted in merged AUC values of 0.99 (No DR versus Mo NPDR) and 0.93 (No DR versus Mi and Mo NPDR). Although further validation with a larger sample size is needed, the 4-protein marker panel (APO4, C7, CLU, and ITIH2) could represent a useful multibiomarker model for detecting the early stages of DR.

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http://dx.doi.org/10.1155/2016/6571976DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4657408PMC
October 2016
23 Reads
2.717 Impact Factor

The small molecule '1-(4-biphenylylcarbonyl)-4-(5-bromo-2-methoxybenzyl) piperazine oxalate' and its derivatives regulate global protein synthesis by inactivating eukaryotic translation initiation factor 2-alpha.

Cell Stress Chaperones 2016 May 12;21(3):485-97. Epub 2016 Feb 12.

Department of Convergence Medicine, Asan Institute for Life Sciences, Asan Medical Center, Convergence Medicine Research Building, 43 gil Olympicro, Pungnapdong, Songpagu, Seoul, 138-736, Republic of Korea.

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http://dx.doi.org/10.1007/s12192-016-0677-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4837177PMC
May 2016
9 Reads
3.163 Impact Factor

Priming with ceramide-1 phosphate promotes the therapeutic effect of mesenchymal stem/stromal cells on pulmonary artery hypertension.

Biochem Biophys Res Commun 2016 Apr 15;473(1):35-41. Epub 2016 Mar 15.

Department of Biomedical Sciences, University of Ulsan College of Medicine, Seoul, South Korea; Department of Physiology, University of Ulsan College of Medicine, Seoul, South Korea. Electronic address:

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http://dx.doi.org/10.1016/j.bbrc.2016.03.046DOI Listing
April 2016
26 Reads
3 Citations
2.300 Impact Factor

Crystal Structure of Streptococcus pyogenes Cas1 and Its Interaction with Csn2 in the Type II CRISPR-Cas System.

Structure 2016 Jan 3;24(1):70-79. Epub 2015 Dec 3.

Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Korea; Center for Food and Bioconvergence, Seoul National University, Seoul 151-921, Korea; Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Korea. Electronic address:

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http://www.cell.com/structure/pdf/S0969-2126(15)00458-X.pdf
Web Search
http://www.cell.com/cms/attachment/2040667351/2054303390/mmc
Web Search
http://linkinghub.elsevier.com/retrieve/pii/S096921261500458
Publisher Site
http://dx.doi.org/10.1016/j.str.2015.10.019DOI Listing
January 2016
12 Reads
3 Citations
5.620 Impact Factor

Measurement of glycosylated alpha-fetoprotein improves diagnostic power over the native form in hepatocellular carcinoma.

PLoS One 2014 13;9(10):e110366. Epub 2014 Oct 13.

Department of Biomedical Sciences, Seoul National University College of Medicine, Seoul, Republic of Korea; Department of Biomedical Engineering, Seoul National University College of Medicine, Seoul, Republic of Korea.

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0110366PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4195728PMC
December 2015
27 Reads
2 Citations
3.234 Impact Factor

Top Down Proteomics Reveals Mature Proteoforms Expressed in Subcellular Fractions of the Echinococcus granulosus Preadult Stage.

J Proteome Res 2015 Nov 28;14(11):4805-14. Epub 2015 Oct 28.

Laboratório de Genômica Estrutural e Funcional, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul , Avenida Bento Gonçalves, 9500 Porto Alegre, Rio Grande do Sul, Brazil.

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http://dx.doi.org/10.1021/acs.jproteome.5b00642DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4638118PMC
November 2015
45 Reads
2 Citations
4.250 Impact Factor

Reducing protein oxidation in low-flow electrospray enables deeper investigation of proteoforms by top down proteomics.

EuPA Open Proteom 2015 Sep 5;8:40-47. Epub 2015 Jun 5.

Departments of Chemistry, Molecular Biosciences and the Proteomics Center of Excellence, Northwestern University, 2145 N. Sheridan Road, Evanston, IL 60208, United States.

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http://dx.doi.org/10.1016/j.euprot.2015.05.005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4704458PMC
September 2015
34 Reads

Integrated approach using multistep enzyme digestion, TiO2 enrichment, and database search for in-depth phosphoproteomic profiling.

Proteomics 2015 Jan 8;15(2-3):618-23. Epub 2014 Oct 8.

Departments of Biomedical Sciences, Medical Research Center, Seoul National University College of Medicine, Seoul, Korea; Biomedical Engineering, Medical Research Center, Seoul National University College of Medicine, Seoul, Korea; Institute of Medical & Biological Engineering, Medical Research Center, Seoul National University College of Medicine, Seoul, Korea.

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http://dx.doi.org/10.1002/pmic.201400102DOI Listing
January 2015
17 Reads
1 Citation
3.810 Impact Factor

Applying label-free quantitation to top down proteomics.

Anal Chem 2014 May 7;86(10):4961-8. Epub 2014 May 7.

Departments of Chemistry, Molecular Biosciences and the Proteomics Center of Excellence , 2145 N. Sheridan Road, Evanston, Illinois 60208, United States.

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http://dx.doi.org/10.1021/ac500395kDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4033644PMC
May 2014
17 Reads
25 Citations
5.640 Impact Factor

Determination of selected reaction monitoring peptide transitions via multiplexed product-ion scan modes.

Rapid Commun Mass Spectrom 2014 Apr;28(7):773-80

Department of Molecular Medicine and Biopharmaceutical Sciences, Graduate School of Convergence Science and Technology and College of Medicine or College of Pharmacy, Seoul National University, Seoul, Republic of Korea.

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http://dx.doi.org/10.1002/rcm.6837DOI Listing
April 2014
31 Reads
2.253 Impact Factor

Development of biomarkers for screening hepatocellular carcinoma using global data mining and multiple reaction monitoring.

PLoS One 2013 22;8(5):e63468. Epub 2013 May 22.

Department of Biomedical Engineering, Seoul National University College of Medicine, Seoul, Republic of Korea.

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0063468PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3661589PMC
April 2014
16 Reads
10 Citations
3.234 Impact Factor

The proto-oncoprotein FBI-1 interacts with MBD3 to recruit the Mi-2/NuRD-HDAC complex and BCoR and to silence p21WAF/CDKN1A by DNA methylation.

Nucleic Acids Res 2013 Jul 8;41(13):6403-20. Epub 2013 May 8.

Department of Biochemistry and Molecular Biology, BK21 Project for Medical Science, Severance Biomedical Research Institute, Yonsei University School of Medicine, 50 Yonsei-Ro, SeoDaeMoon-Gu, Seoul, 120-752, Korea.

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http://dx.doi.org/10.1093/nar/gkt359DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3711425PMC
July 2013
23 Reads
9 Citations
9.112 Impact Factor

Verification of multimarkers for detection of early stage diabetic retinopathy using multiple reaction monitoring.

J Proteome Res 2013 Mar 14;12(3):1078-89. Epub 2013 Feb 14.

Department of Biomedical Engineering, Institute of Medical & Biological Engineering, Medical Research Center, Seoul National University College of Medicine, 28 Yongon-Dong, Seoul 110-799, Korea.

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http://dx.doi.org/10.1021/pr3012073DOI Listing
March 2013
12 Reads
5 Citations
4.250 Impact Factor

Differential proteome profiling using iTRAQ in microalbuminuric and normoalbuminuric type 2 diabetic patients.

Exp Diabetes Res 2012 27;2012:168602. Epub 2012 Mar 27.

Department of Biomedical Sciences, Seoul National University College of Medicine, 28 Yongon-Dong, Seoul 110-799, Republic of Korea.

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http://dx.doi.org/10.1155/2012/168602DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3318901PMC
July 2012
11 Reads
11 Citations
3.540 Impact Factor

Comprehensive phosphoproteome analysis of INS-1 pancreatic β-cells using various digestion strategies coupled with liquid chromatography-tandem mass spectrometry.

J Proteome Res 2012 Apr 14;11(4):2206-23. Epub 2012 Mar 14.

Department of Biomedical Engineering, Institute of Medical & Biological Engineering, Medical Research Center, College of Medicine, Yongon-Dong, Seoul, 110-799 Korea.

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http://pubs.acs.org/doi/10.1021/pr200990b
Publisher Site
http://dx.doi.org/10.1021/pr200990bDOI Listing
April 2012
24 Reads
9 Citations
4.250 Impact Factor

Development of 68Ga-labeled mannosylated human serum albumin (MSA) as a lymph node imaging agent for positron emission tomography.

Nucl Med Biol 2011 Apr 3;38(3):371-9. Epub 2010 Dec 3.

Department of Nuclear Medicine, Institute of Radiation Medicine, Seoul National University College of Medicine, Seoul, South Korea.

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http://dx.doi.org/10.1016/j.nucmedbio.2010.09.010DOI Listing
April 2011
12 Reads
8 Citations
2.412 Impact Factor

Online monitoring of immunoaffinity-based depletion of high-abundance blood proteins by UV spectrophotometry using enhanced green fluorescence protein and FITC-labeled human serum albumin.

Proteome Sci 2010 Dec 1;8:62. Epub 2010 Dec 1.

Department of Biomedical Sciences, Seoul National University, College of Medicine, 28 Yongon-Dong, Seoul 110-799, Korea.

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http://dx.doi.org/10.1186/1477-5956-8-62DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3012035PMC
December 2010
9 Reads
1.730 Impact Factor

Verification of biomarkers for diabetic retinopathy by multiple reaction monitoring.

J Proteome Res 2010 Feb;9(2):689-99

Department of Biomedical Sciences, Cancer Research Institute, and Genome Research Center for Diabetes and Endocrine Disease, Seoul National University College of Medicine, 28 Yongon-Dong, Seoul 110-799, Korea.

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http://dx.doi.org/10.1021/pr901013dDOI Listing
February 2010
4 Reads
14 Citations
4.250 Impact Factor

Preparing multiple-reaction monitoring for quantitative clinical proteomics.

Expert Rev Proteomics 2009 Jun;6(3):225-9

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http://dx.doi.org/10.1586/epr.09.11DOI Listing
June 2009
3 Reads
5 Citations
2.900 Impact Factor

Crystal structure of the N-terminal domain of anaphase-promoting complex subunit 7.

J Biol Chem 2009 May 17;284(22):15137-46. Epub 2008 Dec 17.

Department of Biomedical Sciences and Cancer Research Institute, Seoul National University College of Medicine, 28 Yongon-Dong, Seoul 110-799, Korea.

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http://dx.doi.org/10.1074/jbc.M804887200DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2685695PMC
May 2009
9 Reads
12 Citations
4.573 Impact Factor

Detection of differential proteomes of human beta-cells during islet-like differentiation using iTRAQ labeling.

J Proteome Res 2009 Mar;8(3):1393-403

Departments of Biomedical Sciences and Internal Medicine, Genome Research Center for Diabetes and Endocrine Disease, Seoul National University College of Medicine, 28 Yongon-Dong, Seoul 110-799, Korea.

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http://dx.doi.org/10.1021/pr800765tDOI Listing
March 2009
7 Reads
9 Citations
4.250 Impact Factor

O-GlcNAcylation disrupts glyceraldehyde-3-phosphate dehydrogenase homo-tetramer formation and mediates its nuclear translocation.

Biochim Biophys Acta 2009 Feb 31;1794(2):254-62. Epub 2008 Oct 31.

Department of Biomedical Sciences and Cancer Research Institute, Seoul National University College of Medicine, 28 Yongon-Dong, Chongno-Ku, Seoul 110-799 Republic of Korea.

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http://dx.doi.org/10.1016/j.bbapap.2008.10.003DOI Listing
February 2009
16 Reads
17 Citations

TPR domain of NrfG mediates complex formation between heme lyase and formate-dependent nitrite reductase in Escherichia coli O157:H7.

Proteins 2008 Feb;70(3):900-14

College of Medicine, Seoul National University, Yongon-Dong, Seoul 110-799, Korea.

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http://doi.wiley.com/10.1002/prot.21597
Publisher Site
http://dx.doi.org/10.1002/prot.21597DOI Listing
February 2008
8 Reads
9 Citations
2.630 Impact Factor

Profiling of vitreous proteomes from proliferative diabetic retinopathy and nondiabetic patients.

Proteomics 2007 Nov;7(22):4203-15

Department of Molecular Genomic Medicine, Seoul National University College of Medicine, Yongon-Dong, Seoul, Korea.

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http://dx.doi.org/10.1002/pmic.200700745DOI Listing
November 2007
20 Reads
29 Citations
3.810 Impact Factor

Crystal structure of YrrB: a TPR protein with an unusual peptide-binding site.

Biochem Biophys Res Commun 2007 Sep 5;360(4):784-90. Epub 2007 Jul 5.

College of Medicine, Seoul National University, Yongon-Dong, Seoul 110-799, Republic of Korea.

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http://dx.doi.org/10.1016/j.bbrc.2007.06.129DOI Listing
September 2007
3 Reads
4 Citations
2.300 Impact Factor

Crystal structure of TTC0263, a thermophilic TPR protein from Thermus thermophilus HB27.

Mol Cells 2007 Aug;24(1):27-36

College of Medicine, Seoul National University, Seoul 110-799, Korea.

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August 2007
4 Reads
4 Citations
2.090 Impact Factor

Crystal structure of PilF: functional implication in the type 4 pilus biogenesis in Pseudomonas aeruginosa.

Biochem Biophys Res Commun 2006 Feb 27;340(4):1028-38. Epub 2005 Dec 27.

Division of Molecular Genomic Medicine, College of Medicine, Seoul National University, Yongon-Dong, Seoul 110-799, Republic of Korea.

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http://dx.doi.org/10.1016/j.bbrc.2005.12.108DOI Listing
February 2006
11 Reads
20 Citations
2.300 Impact Factor

Identification and purification of a soluble region of BubR1: a critical component of the mitotic checkpoint complex.

Protein Expr Purif 2005 Nov;44(1):1-9

Division of Molecular Genomic Medicine, College of Medicine, Seoul National University, Yongon-Dong, Seoul 110-799, Korea.

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http://dx.doi.org/10.1016/j.pep.2005.04.020DOI Listing
November 2005
6 Reads
4 Citations
1.700 Impact Factor

Modifying the substrate specificity of penicillin G acylase to cephalosporin acylase by mutating active-site residues.

Biochem Biophys Res Commun 2004 Jun;319(2):486-92

Division of Molecular Genomic Medicine, College of Medicine, Seoul National University, Yongon-Dong, Seoul 110-799, Republic of Korea.

The penicillin G acylase (PGA) and cephalosporin acylase (CA) families, which are members of the N-terminal (Ntn) hydrolases, are valuable for the production of backbone chemicals like 6-aminopenicillanic acid and 7-aminocephalosporanic acid (7-ACA), which can be used to synthesize semi-synthetic penicillins and cephalosporins, respectively. Regardless of the low sequence similarity between PGA and CA, the structural homologies at their active-sites are very high. However, despite this structural conservation, they catalyze very different substrates. PGA reacts with the hydrophobic aromatic side-chain (the phenylacetyl moiety) of penicillin G (PG), whereas CA targets the hydrophilic linear side-chain (the glutaryl moiety) of glutaryl-7-ACA (GL-7-ACA). These different substrate specificities are likely to be due to differences in the side-chains of the active-site residues. In this study, mutagenesis of active-site residues binding the side-chain moiety of PG changed the substrate specificity of PGA to that of CA. This mutant PGA may constitute an alternative source of engineered enzymes for the industrial production of 7-ACA.

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http://dx.doi.org/10.1016/j.bbrc.2004.05.017DOI Listing
June 2004
10 Reads
2.559 Impact Factor

A bound water molecule is crucial in initiating autocatalytic precursor activation in an N-terminal hydrolase.

J Biol Chem 2004 Jan 8;279(1):341-7. Epub 2003 Oct 8.

Division of Molecular Genomic Medicine, College of Medicine, Seoul National University, 28 Yongon-Dong, Seoul 110-799, Korea.

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http://www.jbc.org/content/early/2003/10/08/jbc.M309281200.f
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http://www.jbc.org/cgi/doi/10.1074/jbc.M309281200
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http://dx.doi.org/10.1074/jbc.M309281200DOI Listing
January 2004
5 Reads
8 Citations
4.573 Impact Factor

Modifying the oligomeric state of cyclic amidase and its effect on enzymatic catalysis.

Biochem Biophys Res Commun 2003 Oct;310(2):651-9

Division of Molecular Genomic Medicine, College of Medicine, Seoul National University, Yongon-Dong, Seoul 110-799, South Korea.

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http://dx.doi.org/10.1016/j.bbrc.2003.09.056DOI Listing
October 2003
4 Reads
2.300 Impact Factor

Top co-authors

Dohyun Han
Dohyun Han

Seoul National University College of Medicine

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Jungeun Park
Jungeun Park

Seoul National University

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Jonghwa Jin
Jonghwa Jin

Seoul National University College of Medicine

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Hyeong Gon Yu
Hyeong Gon Yu

Seoul National University College of Medicine

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Yup Kang
Yup Kang

Ajou University School of Medicine

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Kyong Soo Park
Kyong Soo Park

Seoul National University College of Medicine

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Paul M Thomas
Paul M Thomas

University of Illinois at Urbana-Champaign

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