Publications by authors named "Kyung-Yae Hyun"

5 Publications

  • Page 1 of 1

Neuroprotective properties of ethanolic extract of Citrus unshiu Markovich peel through NADPH oxidase 2 inhibition in chemotherapy-induced neuropathic pain animal model.

Phytother Res 2021 Nov 24. Epub 2021 Nov 24.

Department of Clinical Laboratory Science, Dong-Eui University, Busan, Republic of Korea.

The present study aimed to determine the antioxidant effect of Citrus unshiu Markovich (CUM) extract in neuronal cell lines under oxidative stress and to investigate the effect of chemotherapy-induced peripheral neuropathy (CIPN) on the nociceptive response in a preclinical mice model. We tested the inhibition of H O in Neuro2A cells treated with CUM. Experimental animals were treated with oxaliplatin to induce CINP, and then administered oral CUM for 4 weeks in order to observe the effect of CUM. Animals were evaluated weekly for thermal hyperalgesia and digital motor nerve conduction velocity (NCV). Lumbar dorsal root ganglia (DRG) isolated from each animal were evaluated through immunochemical and western blot analysis for nerve damage, inflammatory response, and expression of redox signaling factors. The main mechanisms were determined to be decreased inducible nitric oxide synthase (iNOS) production due to the inhibition of NADPH oxidase 2 (NOX2). To determine the functional role of NOX2 in CINP, we administrated CUM into NOX2-deficient mice with neuropathic pain. Therefore, we suggest that CUM controls the expression levels of inflammatory factors in CINP via NOX2 inactivation. This study demonstrated that a complementary medicine such as CUM might be a potential novel therapeutic agent for the treatment of CINP.
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http://dx.doi.org/10.1002/ptr.7304DOI Listing
November 2021

Neuroprotective Effect of Wurmb by Suppressing TRPV1 Following Sciatic Nerve Crush Injury in a Rat.

Nutrients 2020 Aug 27;12(9). Epub 2020 Aug 27.

Department of Clinical Laboratory Science, Dong-Eui University, Busan 47340, Korea.

Peripheral nerve injury can result in severe functional impairment and decreased quality of life due to loss of sensory and motor function. wurmb (NF) has been used in diverse folk remedies in East Asia. We have previously shown that wurmb extract has antinociceptive and anti-inflammatory effects by suppressing TRPV1 in the sciatic nerve injury. The present study investigated the effects of NF on the control of TRPV1 in relation to neuroprotective effects of a sciatic nerve crush injury. To evaluate the neuroprotective effects, an animal behavior test and a physiological function test were performed. Functional recovery and nerve recovery were improved in the NF and NF + SB (SB366791; TRPV1 antagonist) treated group. In the histomorphology evaluation, the neuronal regenerative effect of NF on the injured sciatic nerve was confirmed via hematoxylin and eosin (H&E) staining. In this study, the NF and NF + SB treated group showed neuroprotective and functional recovery effects from the sciatic nerve crush injury. Furthermore, the expression of NF-κB and iNOS showed a significantly suppressive effect on NF ( < 0.01), SB ( < 0.01), and NF + SB ( < 0.01) treated group at the 7th and 14th day compared to the vehicle group. This study confirmed the neuroprotective effects of NF on suppressing TRPV1 in a sciatic nerve crush injury. The findings of this study establish the effect of NF as a neurotherapeutic agent to protect the peripheral nerve after a sciatic nerve crush injury.
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http://dx.doi.org/10.3390/nu12092618DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7551127PMC
August 2020

Antinociceptive and Anti-Inflammatory Effects of Wurmb by Suppressing TRPV1 in the Sciatic Neuropathies.

Nutrients 2020 Jan 3;12(1). Epub 2020 Jan 3.

Department of Clinical Laboratory Science, Dong-Eui University, Busan 47340, Korea.

Neuropathic pain is generally characterized by sensory abnormalities such as sensory disorders, hyperalgesia, and allodynia. Recent studies have reported that TRPV1 activation is essential for establishing of inflammation in the neuropathy pain models, showing that the expression of this receptor is increased, and contributing to enhanced thermal sensitivity. Wurmb (NF), which was used as a folk remedy, is a plant that is gaining attention due to its various effects. In this study, we investigated the antinociceptive and anti-inflammatory effects of NFE ( Wurmb extracts) by controlling the neurological function of TRPV1. In sciatic crush injury rat models, a significant level of antinociceptive effect was observed in the thermal hyperalgesia test in which NF extracts (NFE 500 mg/kg) were orally administered, daily. Protein quantification of the sciatic nerve and the of the L4-L6 spinal cord showed a decrease of the TRPV1 expression, the inflammatory expression factor, COX2, and proinflammatory factors in the NFE treated groups. Our results indicate that NFE affects antinociceptive and anti-inflammatory by controlling TRPV1 in sciatic neuropathic pain models.
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http://dx.doi.org/10.3390/nu12010135DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7019541PMC
January 2020

Relationship Between Platelet Reactivity and Periprocedural Myonecrosis in Patients Undergoing Percutaneous Coronary Intervention.

J Invasive Cardiol 2019 Dec;31(12):E369-E375

Department of Cardiology, Dong-A University Hospital, 26 Daesingongwon-ro, Seo-gu, Busan, South Korea 602-715.

Background: The impact of platelet reactivity on periprocedural myonecrosis (PMN) in East Asian patients with stable ischemic heart disease or non-ST elevation acute coronary syndrome undergoing percutaneous coronary intervention (PCI) is unclear.

Methods: We enrolled 256 patients with normal high-sensitivity troponin I levels who underwent PCI for stable ischemic heart disease or non-ST elevation acute coronary syndrome. Residual platelet reactivity was assessed by VerifyNow point-of-care P2Y12 assay before PCI and at 18-24 hours following PCI. High platelet reactivity (HPR) was defined using three cut-off scores for platelet reactivity units (PRUs). PMN was defined as a high-sensitivity troponin I elevation of >5x the 99th percentile upper reference limit (URL) in patients with normal baseline values (<99th percentile URL).

Results: The rate of PMN was 55.9% (n = 143) and was significantly higher for pre-PCI and post-PCI PRU values in the fourth quartile compared with those in the first quartile (15% vs 37% [P<.001] and 20% vs 36% [P<.001], respectively). The rate of PMN was higher in patients with HPR, regardless of the criteria used (PRU >208, PRU >235, and PRU >272) and time point. Multivariable analysis revealed that pre-PCI HPR (PRU >208) was an independent predictor of increased risk of PMN (odds ratio, 3.39; 95% confidence interval, 1.87-6.17; P<.001).

Conclusion: Pre-PCI HPR (PRU >208) was associated with an increased risk of PMN in East Asian patients with stable ischemic heart disease or non-ST elevation acute coronary syndrome undergoing PCI. Achievement of optimal platelet reactivity may decrease the risk of PMN.
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December 2019

Inhibitory effect of Acer tegmentosum maxim extracts on P. gingivalis LPS-induced periodontitis.

Arch Oral Biol 2020 Jan 19;109:104529. Epub 2019 Aug 19.

Department of Clinical Laboratory Science, College of Nursing, Healthcare Sciences & Human Ecology, Dong-Eui University, Busan 47340, Republic of Korea. Electronic address:

Objective: Periodontitis disease is a chronic inflammation, and the prevention or treatment of periodontal disease is important for improving oral health and averting systemic diseases.Acer tegmentosum Maxim (ATM) is a type of deciduous tree in Korea. ATM extracts have been traditionally used to treat various dieases. This study investigated the effects of ATM extract on mitigation of periodontitis in vitro and in vivo.

Design: The current study investigated whether extracts ofAcer tegmentosum Maxim (ATM) attenuated periodontitis induced by Porphyromonas gingivalis-derived lipopolysaccharide (LPS) in vitro and in vivo. We used a rat model of experimental periodontitis that received oral administration of 1 mg/kg P. gingivalis-derived LPS for 10 days. Periodontitis models was treated with two different dosages of ATM (30 or 100 mg/kg) during the same period of periodontal induction for histological analysis.

Results: The results indicated that aqueous ATM extracts effectively ameliorated ligature-induced periodontitis through of the antibacterial, anti-oxidative, and anti-inflammatory activities.

Conclusion: These pre-clinical results suggest the need for further studies on the anti-periodontitis effect of ATM in humans. Thus, ATM could be used as a natural anti-periodontitis agent for the treatment of periodontitis.
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http://dx.doi.org/10.1016/j.archoralbio.2019.104529DOI Listing
January 2020
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