Publications by authors named "Kyung Sook Whang"

57 Publications

sp. nov., and sp. nov., isolated from oxidized rice paddy soil.

Int J Syst Evol Microbiol 2019 Jun 25;71(3). Epub 2021 Feb 25.

Department of Microbiology & Resources, Mokwon University, 88 Doanbuk-ro, Seo-gu, Daejeon 35349, Republic of Korea.

Three Gram-stain-negative, motile or non-motile, rod-shaped, facultatively aerobic strains, designated MO-4, NP-34 and NM-18, were isolated from oxidized rice paddy soil in Chungbuk, Republic of Korea. Colonies were circular and convex with entire margins, red in colour on R2A after 3 days at 30 °C. The three strains grew at pH 5.0-10.0 (optimum, pH 8.0), at 15-45 °C (optimum, 30 °C) and at salinities of 0-1.5 % (w/v) NaCl (optimum, 0.4 % NaCl). The results of phylogenetic analyses based on 16S rRNA gene sequences indicated that the three isolates represent members of the genus and strains MO-4 and NP-34 were most closely related to TH057 (97.7 %) and DSM 17366 (97.1 %). NM-18 showed highest 16S rRNA gene sequence similarities to DSM 17366 (98.7 %) and TH057 (96.7 %). Genomic similarities between strains MO-4 and NM-18 and the two type strains of species of the genus based on average nucleotide identity and digital DNA-DNA hybridization values were lower than the species delineation thresholds. The major fatty acids were iso-C ω7 and summed feature 3. The DNA G+C contents of strains MO-4 and NM-18, obtained from genome sequencing data, were 67.6 and 66.6 mol%, respectively. On the basis of these genotypic and phenotypic characteristics, the three strains are assigned to two novel species of the genus , for which the names sp. nov. (type strain MO-4 =KACC 21378=NBRC 114106) and sp. nov. (type strain NM-18=KACC 21379=NBRC 113957) are proposed.
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http://dx.doi.org/10.1099/ijsem.0.004722DOI Listing
June 2019

Altererythrobacter segetis sp. nov., Isolated from Farmland Soil.

Curr Microbiol 2021 Jan 30;78(1):389-396. Epub 2020 Nov 30.

Institute of Microbial Ecology and Resources, Mokwon University, 88 Doanbuk-ro, Seo-gu, Daejeon, 35349, Republic of Korea.

A Gram-stain-negative bacterium, designated YJ20, was isolated from rhizosphere soil of a spinach farmland at Shinan in Korea. Strain YJ20 was found to be aerobic, non-motile rods which can grow at 10-33 °C (optimum, 28 °C), at pH 6.5-8.5 (optimum, pH 6.5-7.5) and in the absence of NaCl. The 16S rRNA gene sequence analysis showed that strain YJ20 belongs to the genus Altererythrobacter with moderate sequence similarities to Altererythrobacter dongtanensis KCTC 22672 (96.8%), Altererythrobacter soli MN-1 (96.6%) and Altererythrobacter xinjiangensis S3-63 (96.5%). The phylogenomic analysis based on the whole-genome sequence demonstrated that strain YJ20 formed a distinct phyletic line with Altererythrobacter soli MN-1 and Altererythrobacter salegens XY-R17 showing average nucleotide identity (ANI) values of 79.4 and 77.5%, respectively. The predominant ubiquinone was identified as Q-10, and the major fatty acids were C ω6c, C ω7c and C 2-OH. The major polar lipids were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidycholin, sphingoglycolipid, an unidentified glycolipid, an unidentified phospholipid and an unidentified lipid. The G+C content of the genome was determined to be 66.3 mol%. On the basis of phenotypic, chemotaxonomic properties and phylogenetic and phylogenomic analyses in this study, strain YJ20 is considered to represent a novel species in the genus Altererythrobacter, for which the name Altererythrobacter segetis sp. nov. is proposed. The type strain is YJ20 (= KACC 19554 = NBRC 113199).
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http://dx.doi.org/10.1007/s00284-020-02294-8DOI Listing
January 2021

Lysobacter telluris sp. nov., isolated from Korean rhizosphere soil.

Arch Microbiol 2021 Jan 12;203(1):287-293. Epub 2020 Sep 12.

Institute of Microbial Ecology and Resources, Mokwon University, 88 Doanbuk-ro, Seo-gu, Daejeon, 35349, Republic of Korea.

A Gram-stain-negative, aerobic, non-motile, non-spore-forming light-yellow-coloured rod-shaped bacterial strain, designated YJ15, was isolated from soil at Bigeum island in Korea. Growth was observed at 10-37 °C (optimum, 28 °C), at pH 6.0-7.5 (optimum, pH 7.0) and in the absence of NaCl. Based on 16S rRNA gene sequence analysis, strain YJ15 was closely related to 'Lysobacter tongrenensis' YS037 (97.8%), Lysobacter pocheonensis Gsoil193 (96.5%) and Lysobacter daecheongensis Dae08 (95.8%) and phylogenetically grouped together with 'Lysobacter tongrenensis' YS037, Lysobacter dokdonensis DS-58 and Lysobacter pocheonensis Gsoil 193. The DNA-DNA relatedness between strain YJ15 and 'Lysobacter tongrenensis' KCTC 52206 was 12% and the phylogenomic analysis based on the whole genome sequence demonstrated that strain YJ20 formed a distinct phyletic line with Lysobacterlter dokdonensis DS-58 showing average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values of 76.3 and 21.3%, respectively. The predominant ubiquinone was identified as Q-8, and polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and two unidentified aminolipids. The major fatty acids were iso-C ω9c, iso-C, iso-C and iso-C. The genomic DNA G + C content was 68.2 mol %. On the basis of phenotypic, chemotaxonomic properties and phylogenetic analyses in this study, strain YJ15 is considered to represent a novel species of the genus Lysobacter, for which the name Lysobacter telluris sp. nov. is proposed. The type strain is YJ15 (= KACC 19552 = NBRC 113197).
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http://dx.doi.org/10.1007/s00203-020-02032-5DOI Listing
January 2021

sp. nov., isolated from paddy soil.

Int J Syst Evol Microbiol 2020 Oct 28;70(10):5304-5311. Epub 2020 Aug 28.

Institute of Microbial Ecology & Resources, Mokwon University, 88, Doanbuk-ro, Seo-gu, Daejeon, Republic of Korea.

Two Gram-stain-positive, facultatively anaerobic, motile, aerobic, rod-shaped and non-spore-forming actinobacteria, strains AO-9 and AO-18, were isolated from paddy soil collected from Daejeon, Republic of Korea. Colonies were smooth, lemon-yellow and circular and 0.5-0.8×2.0-2.4 µm in diameter after 3 days of incubation at 28 °C on tryptic soy agar. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strains AO-9 and AO-18 belonged to the genus , showing the highest sequence similarities to FXJ8.089 (96.6 %), SYSUP0004 (96.5 %), DSM 20111 (96.2 %), DSM 20107 (96.1 %), NEAU-TCZ24 (96.1 %), TR7-06 (96.0 %), JCM 18111 (96.0 %) and less than 96 % to other closely related species. The DNA-DNA hybridization values between strains AO-9 and AO-18 were 87 %. The average nucleotide identity and digital DNA-DNA hybridization values between strain AO-9 and type strains of related species of the genus were 84.0-85.8 % and 20.3-20.9 %, respectively. The major cellular fatty acids are anteiso-C (49.9 %), C (12.9 %) and iso-C (12.1 %). The predominant isoprenoid quinone was MK-9 (H). The polar lipid profile consists of diphosphatidylglycerol, phosphatidylglycerol and one unidentified lipid. The DNA G+C content was 72.9 mol%. Based on its distinctive phenotypic, phylogenetic and chemotaxonomic characteristics, the two strains are considered to represent novel species of the genus , for which the name sp. nov. is proposed. The type strain is AO-9 (=KACC 19069=NBRC 112523).
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http://dx.doi.org/10.1099/ijsem.0.004409DOI Listing
October 2020

gen. nov., sp. nov., a novel bacterium of the family isolated from soil of a farming field.

Int J Syst Evol Microbiol 2020 Sep;70(9):5123-5130

Institute of Microbial Ecology and Resources, Mokwon University, 88 Doanbuk-ro, Seo-gu, Daejeon 35349, Republic of Korea.

A Gram-stain-negative bacterium, designated strain YJ03, was isolated from a spinach farming field soil at Shinan in Korea. Strain YJ03 was found to be an aerobic, non-motile and non-spore-forming bacterium which can grow at 10-33 °C (optimum, 25-28 °C), at pH 6.6-9.5 (optimum, pH 7.0-7.5) and at salinities of 0-1.0 % (w/v) NaCl (optimum, 0 % NaCl). Sequence similarities of the 16S rRNA gene of strain YJ03 with the closely related relatives were in the range 93.9-92.2 %, and the results of phylogenomic analysis indicated that strain YJ03 was clearly separated from species of the genera in the family , showing average nucleotide identity values of 68.8-64.3 %. The predominant isoprenoid quinone was identified as MK-7 and the major fatty acids were iso-C, iso-C 3-OH and an unidentified fatty acid with an equivalent chain-length of 13.565. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, four unidentified aminolipids and six unidentified lipids. The G+C content of the genome was determined to be 41.8 mol%. On the basis of phenotypic and chemotaxonomic properties and phylogenetic and phylogenomic analyses using 16S rRNA gene sequences and whole-genome sequences in this study, strain YJ03 is considered to represent a novel species of a new genus in the family , for which the name gen. nov., sp. nov., is proposed. The type strain of is YJ03 (=KACC 19548=NBRC 113195).
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http://dx.doi.org/10.1099/ijsem.0.004397DOI Listing
September 2020

sp. nov., actinobacterium isolated from farm soil.

Int J Syst Evol Microbiol 2020 Sep 13;70(9):5032-5039. Epub 2020 Aug 13.

Institute of Microbial Ecology and Resources, Mokwon University, 88 Doanbuk-ro, Seo-gu, Daejeon 35349, Republic of Korea.

A Gram-stain-positive actinobacterial strain, designated ANK073, was isolated from rhizosphere soil sampled at a spinach farming field in Shinan, Republic of Korea. Cells of strain ANK073 were found to be aerobic, non-motile, non-spore-forming rods which could grow at 20-40 °C (optimum, 30 °C), at pH 6.0-10.0 (optimum, pH 6.5-7.5) and at salinities of 0-4 % (w/v) NaCl (optimum, 0 % NaCl). The 16S rRNA gene sequence analysis showed that strain ANK073 belongs to the genus with high sequence similarities to CD5 (98.8 %), SJ-23 (98.5 %) and IY07-20 (98.4 %). The phylogenetic analysis indicated that strain ANK073 formed a distinct phyletic line in the genus and the results of DNA-DNA relatedness and phylogenomic analysis based on whole genome sequences demonstrated that strain ANK073 could be separated from its closest relatives in the genus . The strain contained 2,4-diaminobutylic acid, glycine, d-glutamic acid and d-alanine in the peptidoglycan. The predominant menaquinones were identified as MK-12 and MK-11, and the major fatty acids were anteiso-C, anteiso-C  and iso-C. The major polar lipids were identified as diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The G+C content of the genome was determined to be 70.2 mol%. On the basis of its phenotypic and chemotaxonomic properties and the results of phylogenetic and phylogenomic analyses, strain ANK073 is considered to represent a novel species in the genus , for which the name sp. nov. is proposed. The type strain is ANK073 (=KACC 18683=NBRC 111825).
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http://dx.doi.org/10.1099/ijsem.0.004376DOI Listing
September 2020

sp. nov., isolated from spinach farming field soil.

Int J Syst Evol Microbiol 2020 Jun 5;70(6):3905-3911. Epub 2020 Jun 5.

Department of Microbiology & Resources, College of Science & Technology, Mokwon University, 88 Doanbuk-ro, Seo-gu, Daejeon 35349, Republic of Korea.

A Gram-stain-negative bacterium, designated strain YJ09, was isolated from spinach farming field soil at Shinan in the Republic of Korea. Cells of strain YJ09 were found to be strictly aerobic, non-motile, non-spore-forming creamy-yellow rods which can grow at 20-37 °C (optimum, 30 °C), at pH 6.0-9.0 (optimum, pH 7.0-8.0) and at salinities of 0-0.5 % (w/v) NaCl (optimum, 0 % NaCl). The 16S rRNA gene sequence analysis showed that strain YJ09 belongs to the genus with high sequence similarities to GP20-2 (98.0 %), HKS-06 (97.7 %) and JS5 (97.4 %). The results of phylogenetic analysis indicated that strain YJ09 formed a distinct phyletic line in the genus and the results of DNA-DNA relatedness studies demonstrated that strain YJ09 could be separated from its closest relatives in the genus . The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, unidentified glycolipids, an unidentified phospholipid and sphingoglycolipid. The predominant ubiquinone and polyamine components were Q-10 and spermidine, respectively. The major fatty acids were Cω7 C  and Cω7 and/or iso-C 2-OH. The DNA G+C content of this novel isolate was 65.9 mol%. On the basis of phenotypic, chemotaxonomic properties and phylogenetic analyses in this study, strain YJ09 is considered to represent a novel species in the genus , for which the name sp. nov. is proposed. The type strain is YJ09 (=KACC 19551=NBRC 113247).
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http://dx.doi.org/10.1099/ijsem.0.004257DOI Listing
June 2020

sp. nov., a plant promoting bacterium isolated from floodwater of a paddy field.

Int J Syst Evol Microbiol 2020 Mar;70(3):2132-2136

Department of Microbial & Nano Materials, Mokwon University, Daejeon 35349, Republic of Korea.

A Gram-stain-negative bacterium, designated strain PF-30, was isolated from floodwater of a paddy field in South Korea. Strain PF-30 was found to be a strictly aerobic, motile and pink-pigmented rods which can grow at 25-40 °C (optimum, 28 °C), at pH 5.0-9.0 (optimum pH 7.0) and at salinities of 0.5-3.0 % NaCl (optimum 0.5 % NaCl). Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain PF-30 belongs to the genus , showing highest sequence similarity to TU-7 (97.1%) and less than 91.3 % similarity with other members of the family . The average nucleotide identity (ANI) and DNA-DNA relatedness between the strain PF-30 and TU-7 yielded an ANI value of 75.1 % and DNA-DNA relatedness of 11.7±0.7 %, respectively. The major fatty acids were identified as C and C7. The predominant respiratory quinone was identified as Q-10. The DNA G+C content was determined to be 69.9 mol%. The strain PF-30 was observed to produce plant-growth-promoting materials such as indole-3-acetic acid (IAA), siderophore and phytase. On the basis of the results from phylogenetic, chemotaxonomic and phenotypic data, we concluded that strain PF-30 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is PF-30 (=KACC 19985=NBRC 113984).
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http://dx.doi.org/10.1099/ijsem.0.004028DOI Listing
March 2020

gen. nov., sp. nov., an actinobacterium isolated from soil of a farming field.

Int J Syst Evol Microbiol 2020 Mar;70(3):1785-1792

Institute of Microbial Ecology and Resources, Mokwon University, 88 Doanbuk-ro, Seo-gu, Daejeon 35349, Republic of Korea.

A Gram-stain-positive actinobacterial strain, designated YJ01, was isolated from a spinach farming field soil at Shinan in Korea. Strain YJ01 was aerobic, non-motile, non-spore-forming cocci with diameters of 1.5-1.9 µm, and was able to grow at 10-37 °C (optimum, 28-30 °C), at pH 4.5-9.0 (optimum, pH 7.0-8.0) and at salinities of 0-7.5 % (w/v) NaCl (optimum, 1.0 % NaCl). Sequence similarities of the 16S rRNA gene of strain YJ01 with closely related relatives were in the range 96.2-92.8 %, and the results of phylogenomic analysis indicated that strain YJ01 was clearly separated from species of genera in the family showing average nucleotide identity values of 84.2-83.4 %. The predominant isoprenoid quinone was identified as MK-8(H) and the major fatty acids were iso-C, iso-C h, iso-C and anteiso-Cω. The diagnostic diamino acid of the peptidoglycan was ornithine, and the interpeptide bridge was l-Orn-Gly-d-Glu. The major polar lipids were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylserine, an unidentified phosphatidylglycolipid, two unidentified phosphoaminolipids and an unidentified phosphoglycoaminolipid. The G+C content of the genome was 70.1 mol%. On the basis of phenotypic and chemotaxonomic properties and phylogenetic and phylogenomic analyses using 16S rRNA gene sequences and whole-genome sequences, strain YJ01 is considered to represent a novel species of a new genus in the family , for which the name gen. nov. sp. nov. is proposed. The type strain of is YJ01 (=KACC 19547=NBRC 113173).
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http://dx.doi.org/10.1099/ijsem.0.003973DOI Listing
March 2020

sp. nov., a moderately halophilic bacterium isolated from sediment of a crystallizing pond of a saltern.

Int J Syst Evol Microbiol 2020 Jan;70(1):358-363

Department of Microbial & Nano Materials, College of Science & Technology, Mokwon University, 88 Doanbuk-ro, Seo-gu, Daejeon 35349, Republic of Korea.

Two Gram-stain-negative, moderately halophilic bacteria, designated strains ECH52 and KHM46, were isolated from the sediment of a grey saltern located in Sinui island at Shinan, Korea. The isolates were aerobic, non-motile, short rods and grew at 15-45 °C (optimum, 37 °C), at pH 6.0-10.0 (optimum, pH 8.0) and with 3-25 % (w/v) NaCl (optimum, 10 % NaCl). Phylogenetic analyses based on 16S rRNA gene sequences indicated that strains ECH52 and KHM46 belonged to the genus in the family with sequence similarities of 94.3-98.6 % and showed the highest sequence similarity to 2W32 (98.6 %), YIM J21 (94.7%), KHM44 (94.6 %) and YIM D15 (94.3 %). The DNA G+C content of the genomic DNA of strain ECH52 was 40.8 mol%. The predominant isoprenoid quinone was menaquinone-7 (MK-7) and the major cellular fatty acids were iso-Cω9, iso-C, and Cω7 and/or iso-C 2-OH. The major polar lipids were identified as diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, two unidentified glycolipids and four unidentified lipids. Based on the phylogenetic, phenotypic and chemotaxonomic data, strains ECH52 and KHM46 are considered to represent a novel species of the genus for which the name sp. nov. is proposed. The type strain is ECH52 (=KACC 19126=NBRC 112664).
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http://dx.doi.org/10.1099/ijsem.0.003765DOI Listing
January 2020

Paenibacillus pinistramenti sp. nov., isolated from pine litter.

Antonie Van Leeuwenhoek 2020 Feb 12;113(2):155-163. Epub 2019 Sep 12.

Institute of Microbial Ecology and Resources, Mokwon University, 88 Doanbuk-ro, Seo-gu, Daejeon, 35349, Republic of Korea.

A Gram-stain-positive bacterium, designated strain ASL46, was isolated from litter layer of a pine forest located in Anmyondo, Korea. Strain ASL46 was found to be an aerobic, motile, endospore-forming rod which can grow at 20-45 °C (optimum, 37 °C), at pH 6.0-11.0 (optimum, pH 7.0) and at salinities of 0-2% (w/v) NaCl (optimum, 1% NaCl). Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain ASL46 belongs to the genus Paenibacillus, showing highest sequence similarity to P. yonginensis DCY84 (98.3%), P. physcomitrella XB (97.4%) and P. faecis CIP 101062 (96.6%). The average nucleotide identity (ANI) and DNA-DNA relatedness between the strain ASL46 and P. physcomitrella XB and P. yonginensis DCY84 yielded ANI values of 84.6 and 84.5% and DNA-DNA relatedness of 11.7 ± 0.7 and 10.9 ± 0.2%, respectively. The DNA G+C content of the genomic DNA of strain ASL46 was 52.1 mol%. The predominant isoprenoid quinone was identified as menaquinone-7 and the major cellular fatty acids were determined to be anteiso-C, C and iso-C. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, five unidentified aminophospholipids, an unidentified phospholipid and an unidentified glycolipid. The whole-cell sugar was found to be ribose and cell wall peptidoglycan contained meso-diaminopimelic acid. On the basis of phylogenetic analyses, and phenotypic and chemotaxonomic characteristics, strain ASL46 represents a novel species of the genus Paenibacillus, for which the name Paenibacillus pinistramenti sp. nov. is proposed. The type strain is ASL46 (= KACC 18701 = NBRC 111876).
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http://dx.doi.org/10.1007/s10482-019-01325-0DOI Listing
February 2020

sp. nov., a moderately halophilic bacterium isolated from saltern soil.

Int J Syst Evol Microbiol 2019 Oct;69(10):3121-3127

Institute of Microbial Ecology and Resources, Mokwon University, 88 Doanbuk-ro, Seo-gu, Daejeon 35349, Republic of Korea.

A Gram-stain-positive, moderately halophilic bacterium, designated strain BH258, was isolated from solar saltern sediment sampled at Shinan in the Republic of Korea. Cells of strain BH258 were found to be strictly aerobic, motile, endospore-forming rods which could grow at 15-45 °C (optimum, 35 °C), at pH 5.5-9.0 (pH 7.0) and at salinities of 0.5-20 % (w/v) NaCl (7-10%). Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain BH258 belongs to the genus , showing highest sequence similarity to BH30097 (96.1 %), YIM 93624 (95.9 %), B6B (95.6 %) and WSY08-1 (95.1 %). The predominant isoprenoid quinone was identified as menaquinone-7, and the cell-wall peptidoglycan was found to contain -diaminopimelic acid as the diagnostic diamino acid. The major fatty acids were identified as anteiso-C, iso-C, anteiso-C and iso-C. The major polar lipids were identified as phosphatidylglycerol, diphosphatidylglycerol and three unidentified phospholipids. The DNA G+C content of this novel isolate was determined to be 37.35 mol%. On the basis of the results of phylogenetic, phenotypic and chemotaxonomic analyses in this study, strain BH258 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is BH258 (=KACC 18680=NBRC 111875).
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http://dx.doi.org/10.1099/ijsem.0.003599DOI Listing
October 2019

Sphingobium pinisoli sp. nov., isolated from the rhizosphere soil of a Korean native pine tree.

Antonie Van Leeuwenhoek 2019 Jun 18;112(6):815-825. Epub 2018 Dec 18.

Institute of Microbial Ecology and Resources, Mokwon University, 88 Doanbuk-ro, Seo-gu, Daejeon, 35349, Republic of Korea.

A Gram-stain negative, aromatic compound degrading bacterium, designated strain ASA28, was isolated from the rhizosphere soil of a pine tree at Anmyon island, Taean in Korea. Strain ASA28 was found to be strictly aerobic, non-motile, short rods which can grow at 15-28 °C (optimum, 25-28 °C), at pH 5.0-11.0 (optimum, pH 7.0) and at salinities of 0-1.0% (w/v) NaCl (optimum, 0% NaCl). Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain ASA28 belongs to the genus Sphingobium, showing high sequence similarity to Sphingobium scionense WP01 (97.8%), Sphingobium vermicocomposti VC-230 (96.8%), Sphingobium yanoikuyae ATCC 51230 (96.5%) and Sphingobium herbicidovorans MH (95.6%). The predominant ubiquinone and polyamine components were identified as Q-10 and spermidine, respectively. The major fatty acids were identified as Cω7c, C, C 2-OH and Cω7c and/or C iso 2-OH. The major polar lipids were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylcholine, sphingoglycolipid, phosphoglycolipid, four unidentified aminophospholipids, an unidentified aminolipid, two unidentified phospholipids and six unidentified lipids. The DNA G+C content of this novel isolate was determined to be 63.0 mol%. DNA-DNA relatedness between strain ASA28 and S. herbicidovorans KCTC 2939, S. vermicocomposti DSM 21299 and S. scionense DSM 19371 was determined to be 32 ± 5%, 30 ± 4% and 25 ± 5%, respectively. On the basis of the phylogenetic, phenotypic and chemotaxonomic analyses in this study, strain ASA28 is considered to represent a novel species of the genus Sphingobium, for which the name Sphingobium pinisoli sp. nov. is proposed. The type strain is ASA28 (= KACC 18700 = NBRC 112246).
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http://dx.doi.org/10.1007/s10482-018-01215-xDOI Listing
June 2019

Streptomyces fuscigenes sp. nov., isolated from bamboo (Sasa borealis) litter.

Int J Syst Evol Microbiol 2018 May 14;68(5):1541-1545. Epub 2018 Mar 14.

Department of Microbial and Nano Materials, Mokwon University, Daejeon 302-729, Republic of Korea.

Three actinomycetes strains, designated JBL-5, JBL-14 and JBL-20, were isolated from bamboo (Sasa borealis) litter in Damyang, Republic of Korea. Morphological, chemotaxonomic and phylogenetic analyses demonstrated that the three strains belong to the genus Streptomyces. Microscopic observation revealed that the three strains produced Spirales spore chains with smooth surfaces. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that these strains showed the highest sequence similarity to Streptomyces gelaticus NRRL B-2928 (97.8 %), Streptomyces pulveraceus LMG 20322 (97.7 %), Streptomyces intermedius NBRC 13049 (97.7 %), Streptomyces althioticus NRRL B-3981 (97.7 %) and Streptomyces matensis NBRC 12889 (97.7 %). The DNA-DNA hybridization values between strains JBL-5, JBL-14 and JBL-20 were 91.2-92.4 %, and the values between the three strains and their close phylogenetic relatives were also below 70 %. The predominant menaquinones were MK-9 (H4) and MK-9 (H6). The cell wall contained ll-diaminopimelic acid and the whole-cell sugars were arabinose and xylose. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositolmannoside, two unidentified aminolipids, three unidentified aminophospholipids, one unidentified glycolipid and one unidentified lipid. The major cellular fatty acids were iso-C15 : 0, anteiso-C15 : 0, iso-C14 : 0, C18 : 1ω7c and iso-C16 : 0. The DNA G+C contents were 71.8-72.4 mol%. On the basis of phylogenetic analyses and physiological and biochemical characterization, strains JBL-5, JBL-14 and JBL-20 are considered to represent a novel species of the genus Streptomyces, for which the name Streptomyces fuscigenes sp. nov. is proposed. The type strain is JBL-20 (=KACC 18269=NBRC 110629).
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http://dx.doi.org/10.1099/ijsem.0.002706DOI Listing
May 2018

Erratum: Aliifodinibius salicampi sp. nov., a moderately halophilic bacterium isolated from a grey saltern.

Int J Syst Evol Microbiol 2018 02;68(2):692

Department of Microbial and Nano Materials, College of Science and Technology, Mokwon University, 88 Doanbuk-ro, Seo-gu, Daejeon 35349, Republic of Korea.

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http://dx.doi.org/10.1099/ijsem.0.002572DOI Listing
February 2018

Salirhabdus salicampi sp. nov., a halotolerant bacterium isolated from a saltern.

Int J Syst Evol Microbiol 2017 Nov 25;67(11):4578-4583. Epub 2017 Sep 25.

Department of Microbial and Nano Materials, College of Science and Technology, Mokwon University, 88 Doanbuk-ro, Seo-gu, Daejeon 35349, Republic of Korea.

A Gram-stain-positive, halotolerant bacterium, designated strain BH128, was isolated from soil of a saltern located at Bigeum Island in south-west Korea. Cells were aerobic, motile, spore-forming rods and grew at 15-53 °C (optimum, 35 °C), at pH 5.5-9.0 (optimum, pH 7.0) and at salinities of 0-16 % (w/v) NaCl (optimum, 8 % NaCl). The predominant isoprenoid quinone was menaquinone-7 (MK-7), and the cell-wall peptidoglycan type was A1γ, with meso-diaminopimelic acid as the diagnostic diamino acid. The major fatty acids were iso-C15 : 0, anteiso-C15 : 0, iso-C17 : 0 and anteiso-C17 : 0. The polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and four unknown phospholipids. The DNA G+C content was 36.5 mol%. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain BH128 belonged to the genus Salirhabdus and showed highest similarity to Salirhabduseuzebyi CVS-14 (95.8 %). On the basis of the phylogenetic, phenotypic and chemotaxonomic analyses in this study, strain BH128 is considered to represent a novel species of the genus Salirhabdus, for which the name Salirhabdussalicampi sp. nov. is proposed. The type strain is BH128 (=KACC 18690=NBRC 111874).
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http://dx.doi.org/10.1099/ijsem.0.002334DOI Listing
November 2017

Mycobacterium aquiterrae sp. nov., a rapidly growing bacterium isolated from groundwater.

Int J Syst Evol Microbiol 2017 Oct 18;67(10):4104-4110. Epub 2017 Sep 18.

Department of Microbial & Nano Materials, College of Science & Technology, Mokwon University, 88 Doanbuk-ro, Seo-gu, Daejeon 302-318, Republic of Korea.

A strain representing a rapidly growing, Gram-stain-positive, aerobic, rod-shaped, non-motile, non-sporulating and non-pigmented species of the genus Mycobacterium, designated strain S-I-6, was isolated from groundwater at Daejeon in Korea. The strain grew at temperatures between 10 and 37 °C (optimal growth at 25 °C), between pH 4.0 and 9.0 (optimal growth at pH 7.0) and at salinities of 0-5 % (w/v) NaCl, growing optimally with 2 % (w/v) NaCl. Phylogenetic analyses based on multilocus sequence analysis of the 16S rRNAgene, hsp65, rpoB and the 16S-23S internal transcribed spacer indicated that strain S-I-6 belonged to the rapidly growing mycobacteria, being most closely related to Mycobacterium sphagni. On the basis of polyphasic taxonomic analysis, the bacterial strain was distinguished from its phylogenetic neighbours by chemotaxonomic properties and other biochemical characteristics. DNA-DNA relatedness among strain S-I-6 and the closest phylogenetic neighbour strongly support the proposal that this strain represents a novel species within the genus Mycobacterium, for which the name Mycobacterium aquiterrae sp. nov. is proposed. The type strain is S-I-6 (=KACC 17600=NBRC 109805=NCAIM B 02535).
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http://dx.doi.org/10.1099/ijsem.0.002261DOI Listing
October 2017

Aliifodinibius salicampi sp. nov., a moderately halophilic bacterium isolated from a grey saltern.

Int J Syst Evol Microbiol 2017 Aug 31;67(8):2598-2603. Epub 2017 Jul 31.

Department of Microbial and Nano Materials, College of Science and Technology, Mokwon University, 88 Doanbuk-ro, Seo-gu, Daejeon 35349, Republic of Korea.

Three strains of a Gram-stain-negative, moderately halophilic bacterium, designated strain KHM44T, KHM29 and HHM4, were isolated from the sediment of a grey saltern located on Sinui island at Shinan, Korea. The isolates were aerobic, non-motile rods and grew at 15-50 °C (optimum, 37 °C), at pH 6.0-9.0 (optimum, pH 8.0) and at salinities of 3-25 % (w/v) NaCl (optimum, 10 % NaCl). The predominant isoprenoid quinone was menaquinone-7 (MK-7), and the major fatty acids were iso-C15 : 0, C16 : 1ω7c and/or iso-C15 : 0 2-OH, iso-C17 : 1ω9c and anteiso-C15 : 0. The polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine and glycolipid. The DNA G+C contents were 48.5-48.7 mol%. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strains KHM44T, KHM29 and HHM4 belonged to the genus Aliifodinibius in the family Balneolaceae, with sequence similarities of 95.1-97.2 % to members of this genus. Levels of DNA-DNA relatedness between strain KHM44T and the type strains of the other species of the genus Aliifodinibius ranged from 35.4 to 48.0 %. On the basis of polyphasic analysis from this study, strains KHM44T, KHM29 and HHM4 are considered to represent a novel species of the genus Aliifodinibius, for which the name Aliifodinibius salicampi sp. nov. is proposed. The type strain is KHM44T (=KACC 19060T=NBRC 112531T).
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http://dx.doi.org/10.1099/ijsem.0.001981DOI Listing
August 2017

Micromonospora fulva sp. nov., isolated from forest soil.

Int J Syst Evol Microbiol 2017 Jun 20;67(6):1746-1751. Epub 2017 Jun 20.

Department of Microbial & Nano Materials, Mokwon University, Daejeon 302-729, Republic of Korea.

A novel actinobacterium, designated strain UDF-1T, was isolated from forest soil in Chungnam, South Korea, and its taxonomic position was investigated using a polyphasic approach. Strain UDF-1T formed a branched brownish-orange substrate mycelium with spherical or oval spores. No aerial mycelium was formed. Comparative 16S rRNA gene sequence analysis indicated that strain UDF-1T belongs to the genus Micromonospora, showing the highest sequence similarity to Micromonospora palomenae NEAU-CX1T (99.2 % 16S rRNA gene sequence similarity), 'Micromonospora maoerensis' NEAU-MES19 (99.0 %), Micromonospora endolithica DSM 44398T (98.8 %) and Micromonospora matsumotoense IMSNU 22003T (98.8 %). The predominant menaquinones of strain UDF-1T were MK-10 (H4) and MK-10 (H6). The cell wall contained meso-diaminopimelic acid and the whole-cell sugars were arabinose and xylose. The major polar lipids were phosphatidylinositol, diphosphatidylglycerol and phosphatidylethanolamine. The major cellular fatty acids were iso-C16 : 0, anteiso-C15 : 0 and iso-C15 : 0. The genomic DNA G+C content was 73.1 mol%. DNA-DNA relatedness between strain UDF-1T and closely related type strains in the genus Micromonospora was below 30 %. On the basis of the polyphasic analysis conducted in this study, strain UDF-1T represents a novel species of the genus Micromonospora, for which the name Micromonospora fulva sp. nov. is proposed. The type strain is UDF-1T (=KACC 18696T=NBRC 111826T).
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http://dx.doi.org/10.1099/ijsem.0.001858DOI Listing
June 2017

Rhodanobacter rhizosphaerae sp. nov., isolated from soil of ginseng rhizosphere.

Int J Syst Evol Microbiol 2017 May 30;67(5):1387-1392. Epub 2017 May 30.

Institute of Microbial Ecology and Resources, Mokwon University, 88 Doanbuk-ro, Seo-gu, Daejeon 35349, Republic of Korea.

A Gram-stain-negative, aerobic, non-motile, non-spore-forming, yellow and rod-shaped bacterium, designated strain CR164T, was isolated from the rhizosphere soil of a ginseng field at Geumsan in Korea. CR164T grew at between 15 and 37 °C (optimal growth at 28 °C), between pH 6.0 and 9.0 (optimal growth at pH 7.0) and at salinities of 0-1.0 % (w/v) NaCl, growing optimally in the absence of NaCl. The results of phylogenetic analyses based on 16S rRNA gene sequences indicated that CR164T represents a member of the genus Rhodanobacter, showing the highest sequence similarity to Rhodanobactercaeni MJ01T (98.5 %), Rhodanobacter ginsenosidimutans Gsoil 3054T (98.4 %), Rhodanobacter thiooxydans LCS2T (98.3 %), Rhodanobacter lindaniclasticus RP5557T (98.1 %), Rhodanobacter denitrificans 2APBS1T (98.0 %), Rhodanobacter fulvus Jip2T (97.6 %), Rhodanobacter soli DCY45T (97.3 %) and 'Rhodanobacterxiangquanii' BJQ-6 (97.0 %). The major fatty acids were iso-C17 : 1ω9c (21.8 %), iso-C15 : 0 (12.1 %), iso-C11 : 0 (11.9 %) and iso-C16 : 0 (11.1 %). The predominant ubiquinone was Q-8. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The G+C content of the genomic DNA was 62.3 mol%. DNA-DNA relatedness between CR164T and the type strains of eight other species of the genus ranged from 51 to 9 %. On the basis of the polyphasic analysis, CR164T represents a novel species of the genus Rhodanobacter, for which the name Rhodanobacter rhizosphaerae sp. nov. is proposed. The type strain is CR164T (=KACC 18699T=NBRC 111845T).
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http://dx.doi.org/10.1099/ijsem.0.001825DOI Listing
May 2017

Terrimonas rhizosphaerae sp. nov., isolated from ginseng rhizosphere soil.

Int J Syst Evol Microbiol 2017 Feb 16;67(2):391-395. Epub 2017 Mar 16.

Institute of Microbial Ecology and Resources, Mokwon University, 88 Doanbuk-ro, Seo-gu, Daejeon 35349, Republic of Korea.

The novel isolate belonging to the genus Terrimonas, designated CR94T, was isolated from rhizosphere soil of a ginseng field in Geumsan, Korea. Cells of strain CR94T were strictly aerobic, Gram-stain-negative, non-motile, non-filamentous single rods. Growth was observed at 10-37 °C (optimum 28 °C) and at pH 4.0-10.0 (optimum pH 6.0). Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain CR94T belonged to the genus Terrimonas, showing highest sequence similarity to Terrimonas lutea DYT (97.3 %), Terrimonas pekingensis QHT (97.1 %), Terrimonas aquatica RIB1-6T (95.6 %), Terrimonas rubra M-8T (94.7 %) and Terrimonas ferruginea ATCC 13524T (93.8 %). DNA-DNA relatedness values between strain CR94T and T. lutea KACC 13047T, T. pekingensis KACC 18795T, T. ferruginea KACC 11310T and T. aquatica LMG 24825T were 30.5, 28.9, 17.8 and 13.5 %, respectively. The DNA G+C content was 46.5 mol% and the major respiratory quinone was menaquinone-7 (MK-7). The major cellular fatty acids of strain CR94T were iso-C15:1 G and iso-C15 : 0. On the basis of the polyphasic analysis, strain CR94T represents a novel species of the genus Terrimonas, for which the name Terrimonas rhizosphaerae sp. nov. is proposed. The type strain is CR94T (=KACC 17564T=NCAIM B 025317T).
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http://dx.doi.org/10.1099/ijsem.0.001639DOI Listing
February 2017

Marinobacter halotolerans sp. nov., a halophilic bacterium isolated from a saltern crystallizing pond.

Int J Syst Evol Microbiol 2017 Feb 16;67(2):460-465. Epub 2017 Mar 16.

Institute of Microbial Ecology & Resources, Mokwon University, Daejeon 302-729, Republic of Korea.

A Gram-stain-negative, moderately halophilic, motile bacterium, designated strain CP12T, was isolated from a crystallizing pond of a saltern of the Yellow Sea in Korea. Cells of strain CP12T were non-spore-forming rods and produced whitish-yellow colonies. Growth was observed at 10-37 °C (optimum 37 °C), at pH 6.0-9.0 (optimum pH 7.0), and in the presence of 0.5-20 % (w/v) NaCl (optimum 3 %). Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain CP12T was closely related to Marinobacter flavimaris SW-145T (98.4 % 16S rRNA gene sequence similarity), Marinobacter algicola DG893T (98.2 %), Marinobacter adhaerens HP15T (98.2 %), Marinobacter salsuginis SD-14BT (97.9 %), Marinobacter salarius R9SW1T (97.6 %) and Marinobacter lipolyticus SM19T (97.1 %). DNA-DNA hybridization studies showed values lower than 18.6 % between strain CP12T and any of these species. The predominant respiratory isoprenoid quinone was ubiquinone-9 and the major cellular fatty acids of strain CP12T were C16 : 0, C12 : 0 3-OH, C12 : 0, Summed feature 3, C16 : 0 10-methyl and C18 : 1ω9c. On the basis of phenotypic properties, and phylogenetic and chemotaxonomic data, it is evident that strain CP12T represents a novel species of the genus Marinobacter, for which the name Marinobacter halotolerans sp. nov. is proposed. The type strain is CP12T (=KACC 18381T=NBRC 110910T).
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http://dx.doi.org/10.1099/ijsem.0.001653DOI Listing
February 2017

Halomonas sediminicola sp. nov., a moderately halophilic bacterium isolated from a solar saltern sediment.

Int J Syst Evol Microbiol 2016 Oct 5;66(10):3865-3872. Epub 2016 Jul 5.

Institute of Microbial Ecology and Resources, Mokwon University, 88 Doanbuk-ro, Seo-gu, Daejeon 302-318, Republic of Korea.

A Gram-stain-negative, moderately halophilic bacterium, designated strain CPS11T, was isolated from the sediment of a solar pond located in Shinan, Korea. Strain CPS11T was a strictly aerobic, motile, straight rod-shaped bacterium that grew at pH 5.0-9.0 (optimum, pH 7.0-8.0), at 10-37 °C (optimum, 28 °C) and at salinities of 1-20 % (w/v) NaCl (optimum, 10 % NaCl). Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain CPS11T belonged to the genus Halomonas, with sequence similarity of 98.5-94.3 % to existing type strains, showing highest sequence similarity to Halomonasfontilapidosi 5CRT (98.5 %), Halomonasventosae Al12T (98.5 %), Halomonascampaniensis 5AGT (98.2 %), Halomonas huangheensis BJGMM-B45T (98.0 %), Halomonas alimentaria YKJ-16T (98.0 %), Halomonas mongoliensis Z-7009T (97.8 %), Halomonas shengliensis SL014B-85T (97.5 %) and Halomonas cupida DSM 4740T (97.5 %). The predominant ubiquinone was Q-9. The major fatty acids were C19 : 0 cyclo ω8c, C16 : 1ω7c and/or iso-C15 : 0 2-OH, C16 : 0, C17 : 0 cyclo, C12 : 0 3-OH and C18 : 1ω7c. The polar lipids were phosphatidylglycerol, phosphatidylethanolamine, aminophospholipid, an unknown phospholipid and unknown lipids. The DNA G+C content of this novel isolate was 64.3 mol%. Levels of DNA-DNA relatedness between strain CPS11T and the type strains of ten other species of the genus ranged from 50 to 21 %. On the basis of the polyphasic analysis conducted in this study, strain CPS11T represents a novel species of the genus Halomonas, for which the name Halomonas sediminicola sp. nov. is proposed. The type strain is CPS11T(=KACC 18262T=NBRC 110636T).
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http://dx.doi.org/10.1099/ijsem.0.001278DOI Listing
October 2016

Streptomyces rhizosphaerihabitans sp. nov. and Streptomyces adustus sp. nov., isolated from bamboo forest soil.

Int J Syst Evol Microbiol 2016 Sep 13;66(9):3573-3578. Epub 2016 Jun 13.

Department of Microbial & Nano Materials, Mokwon University, Daejeon 302-729, Republic of Korea.

Three novel isolates belonging to the genus Streptomyces, designated JR-35T, JR-46 and WH-9T, were isolated from bamboo forest soil in Damyang, Korea. The 16S rRNA gene sequences of strains JR-35T and JR-46 showed highest similarities with Streptomyces olivochromogenes NBRC 3178T (99.1 %), Streptomyces siamensis KC-038T (98.9 %), Streptomyces chartreusis NBRC 12753T (98.9 %), Streptomyces resistomycificus NRRL ISP-5133T (98.9 %) and Streptomyces bobili JCM 4627T (98.8 %), and strain WH-9Tshowed highest sequence similarities with Streptomyces. bobili JCM 4627T (99.2 %), Streptomyces phaeoluteigriseus NRRL ISP-5182T (99.2 %), Streptomyces alboniger NBRC 12738T (99.2 %), Streptomyces galilaeus JCM 4757T (99.1 %) and Streptomyces pseudovenezuelae NBRC 12904T (99.1 %). The predominant menaquinones were MK-9 (H6) and MK-9 (H8). The major fatty acids were anteiso-C15 : 0, iso-C16 : 0, iso-C14 : 0 and iso-C15 : 0 for strains JR-35T and JR-46 and anteiso-C15 : 0, iso-C15 : 0 and iso-C16 : 0 for strain WH-9T. The G+C content of the genomic DNA of strains JR-35T, JR-46 and WH-9T were 69.4, 74.4 and 74.1 mol%, respectively. Based on the phenotypic and genotypic data, the three strains are assigned to two novel species of the genus Streptomyces, for which the names Streptomyces rhizosphaerihabitans sp. nov. (type stain JR-35T=KACC 17181T=NBRC 109807T) and Streptomyces adustus sp. nov. (type strain WH-9T=KACC 17197T=NBRC 109810T) are proposed.
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http://dx.doi.org/10.1099/ijsem.0.001236DOI Listing
September 2016

Denitratimonas tolerans gen. nov., sp. nov., a denitrifying bacterium isolated from a bioreactor for tannery wastewater treatment.

Antonie Van Leeuwenhoek 2016 Jun 23;109(6):785-92. Epub 2016 Apr 23.

Department of Microbial & Nano Materials, College of Science & Technology, Mokwon University, 88 Doanbuk-ro, Seo-gu, Daejeon, 35349, Republic of Korea.

A denitrifying bacterium, designated strain E4-1(T), was isolated from a bioreactor for tannery wastewater treatment, and its taxonomic position was investigated using a polyphasic approach. Strain E4-1(T), a facultative anaerobic bacterium, was observed to grow between 0 and 12 % (w/v) NaCl, between pH 3.0 and 12.0. Cells were found to be oxidase-positive and catalase-negative. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain E4-1(T) forms a distinct lineage with respect to closely related genera in the family Xanthomonadaceae, and is closely related to Chiayiivirga, Aquimonas and Dokdonella, and the levels of 16S rRNA gene sequence similarity with respect to the type species of related genera are less than 93.9 %. The predominant respiratory quinone was determined to be ubiquinone-8 (Q-8) and the major cellular fatty acids were determined to be iso-C15:0, iso-C17:1 ω9c, iso-C11:0 and iso-C11:0 3OH. Based on physiological, biochemical and chemotaxonomic properties together with results of comparative 16S rRNA gene sequence analysis, strain E4-1(T) is considered to represent a novel species in a new genus, for which the name Denitratimonas tolerans gen. nov., sp. nov. is proposed. The type strain is E4-1(T) (=KACC 17565(T) = NCAIM B 025327(T)).
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http://dx.doi.org/10.1007/s10482-016-0678-5DOI Listing
June 2016

Halobacillus salicampi sp. nov., a moderately halophilic bacterium isolated from a solar saltern sediment.

Antonie Van Leeuwenhoek 2016 May 4;109(5):713-20. Epub 2016 Mar 4.

Department of Microbial & Nano Materials, College of Science & Technology, Mokwon University, 88 Doanbuk-ro, Seo-gu, Daejeon, 302-318, Republic of Korea.

A Gram-positive, moderately halophilic bacterium, designated strain TGS-15(T), was isolated from the sediment of a solar saltern pond located in Shinan, Korea. Strain TGS-15(T) was found to be a strictly aerobic, non-motile rod which can grow at pH 6.0-10.0 (optimum, pH 9.0), at 20-35 °C (optimum, 28 °C) and at salinities of 1-20 % (w/v) NaCl (optimum, 9 % NaCl). Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain TGS-15(T) belongs to the genus Halobacillus, with sequence similarity of 98.5-96.0 % to known type strains, showing high sequence similarity to Halobacillus locisalis MSS-155(T) (98.5 %), Halobacillus faecis IGA7-4(T) (98.2 %) and Halobacillus alkaliphilus FP5(T) (98.0 %), and less than 98.0 % sequence similarity to other currently recognised type strains of the genus. The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, an unidentified glycolipid and an unidentified lipid. The cell wall peptidoglycan was found to be based on L-Orn-D-Asp, the predominant isoprenoid quinone was identified as menaquinone-7 (MK-7) and the major fatty acids were identified as anteiso-C15:0, iso-C15:0, anteiso-C17:0 and C16:1 ω7c alcohol. The DNA G+C content of this novel isolate was determined to be 45.3 mol %. Levels of DNA:DNA relatedness between strain TGS-15(T) and the type strains of 13 other species of the genus ranged from 52 to 9 %. On the basis of the polyphasic analysis conducted in this study, strain TGS-15(T) is concluded to represent a novel species of the genus Halobacillus, for which the name Halobacillus salicampi sp. nov. is proposed. The type strain is TGS-15(T) (=KACC 18264(T) = NBRC 110640(T)).
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http://dx.doi.org/10.1007/s10482-016-0672-yDOI Listing
May 2016

Catenulispora fulva sp. nov., isolated from forest soil.

Int J Syst Evol Microbiol 2016 Jan 26;66(1):271-275. Epub 2015 Oct 26.

Institute of Microbial Ecology & Resources, Mokwon University, Daejeon 302-729, Republic of Korea.

An actinomycete strain, designated SA-246T, was isolated from a forest soil sample collected from Chungnam, South Korea. Applying a polyphasic approach, the isolate was identified as a member of the genus Catenulispora using morphological and chemotaxonomic characteristics, including the presence of ll-diaminopimelic acid, glutamic acid, alanine and glycine in the peptidoglycan. Whole-cell hydrolysates contained predominantly rhamnose, mannose, ribose, arabinose, galactose and glucose. The major menaquinones were MK-9(H4), MK-9(H6) and MK-9(H8). 16S rRNA gene sequence analysis revealed that strain SA-246T belongs to the genus Catenulispora, showing the highest sequence similarity to Catenulispora yoronensis TT N02-20T (98.7 % 16S rRNA gene sequence similarity), Catenulispora subtropica TT 99-48T (98.2 %), Catenulispora graminis BR-34T (97.4 %), Catenulispora rubra Aac-30T (97.4 %) and Catenulispora acidiphila ID139908T (97.3 %). On the basis of polyphasic analysis from this study, strain SA-246T represents a novel species of the genus Catenulispora, for which the name Catenulispora fulva sp. nov. is proposed. The type strain is SA-246T ( = KACC 17878T = NBRC 110074T).
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http://dx.doi.org/10.1099/ijsem.0.000711DOI Listing
January 2016

Halomonas salicampi sp. nov., a halotolerant and alkalitolerant bacterium isolated from a saltern soil.

Int J Syst Evol Microbiol 2015 Dec 1;65(12):4792-4799. Epub 2015 Oct 1.

Institute of Microbial Ecology and Resources, Mokwon University, 88 Doanbuk-ro, Seo-gu, Daejeon 302-318, Republic of Korea.

A Gram-stain-negative, halotolerant and alkalitolerant bacterium, designated strain BH103T, was isolated from saltern soil in Gomso, Korea. Cells of strain BH103T were strictly aerobic, motile, straight rods and grew at pH 7.0-10.8 (optimum, pH 8.5), at 10-55 °C (optimum, 28 °C) and at salinities of 0-23 % (w/v) NaCl (optimum, 14 % NaCl). Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain BH103T belongs to the genus Halomonas, showing highest sequence similarity to Halomonas boliviensis LC1T (97.7 %), Halomonas neptunia Eplume1T (97.7 %), Halomonas variabilis IIIT (97.7 %), Halomonas alkaliantarctica CRSST (97.7 %), Halomonas olivaria TYRC17T (97.5 %), Halomonas titanicae BH1T (97.2 %) and Halomonas sulfidaeris Esulfide1T (96.2 %). The predominant ubiquinone was Q-9. The major fatty acids were C18 : 1ω7c, C16 : 1ω7c and/or iso-C15 : 0 2-OH, C16 : 0 and C12 : 0 3-OH. The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, aminophospholipid and an unknown phospholipid. The DNA G+C content of this novel isolate was 54.7 mol%. DNA-DNA relatedness between strain BH103T and H. boliviensis KACC 16615T, H. neptunia KCTC 2888T, H. variabilis KCTC 2889T, H. alkaliantarctica KCTC 22844T, H. olivaria DSM 19074T, H. titanicae JCM 16411T and H. sulfidaeris DSM 15722T was 45, 41, 39, 32, 38, 45 and 35 %, respectively. On the basis of polyphasic analysis from this study, strain BH103T represents a novel species of the genus Halomonas, for which the name Halomonas salicampi sp. nov. is proposed. The type strain is BH103T ( = KACC 17609T = NBRC 109914T = NCAIM B 02528T).
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http://dx.doi.org/10.1099/ijsem.0.000650DOI Listing
December 2015

Halobacillus sediminis sp. nov., a moderately halophilic bacterium isolated from a solar saltern sediment.

Int J Syst Evol Microbiol 2015 Dec 10;65(12):4434-4440. Epub 2015 Sep 10.

Institute of Microbial Ecology and Resources, Mokwon University, 88 Doanbuk-ro, Seo-gu, Daejeon 302-318, Republic of Korea.

A Gram-staining-positive, moderately halophilic bacterium, designated strain NGS-2T, was isolated from sediment of a solar saltern pond located in Shinan, Korea. Strain NGS-2T was a strictly aerobic, non-motile rod that grew at pH 5.0-10.0 (optimum, pH 8.0), at 10-30 °C (optimum, 28 °C) and in the presence of 1-20 % (w/v) NaCl (optimum, 10 % NaCl). Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain NGS-2T belonged to the genus Halobacillus, with sequence similarity of 98.4-95.8 % to existing type strains, showing the highest sequence similarity to Halobacillus dabanensis D-8T (98.4 %), H. litoralis SL-4T (98.4 %), H. trueperi SL-5T (98.2 %), H. faecis IGA7-4T (98.2 %), H. profundi IS-Hb4T (98.1 %) and H. mangrovi MS10T (98.0 %). The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidyl-N-methylethanolamine and an unknown glycolipid. The cell-wall peptidoglycan was based on l-Orn-d-Asp, the predominant isoprenoid quinone was menaquinone 7 (MK-7) and the major fatty acids were anteiso-C15: 0 and anteiso-C17: 0. The DNA G+C content of the novel isolate was 45.0 mol%. Levels of DNA-DNA relatedness between strain NGS-2T and the type strains of 12 other species of the genus ranged from 32 to 3 %. On the basis of the polyphasic analysis conducted in this study, strain NGS-2T represents a novel species of the genus Halobacillus, for which the name Halobacillus sediminis sp. nov. is proposed. The type strain is NGS-2T ( = KACC 18263T = NBRC 110639T).
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http://dx.doi.org/10.1099/ijsem.0.000595DOI Listing
December 2015

Streptomyces sasae sp. nov., isolated from bamboo (Sasa borealis) rhizosphere soil.

Int J Syst Evol Microbiol 2015 Oct;65(10):3547-3551

Institute of Microbial Ecology & Resources, Mokwon University, Daejeon 302-729, Republic of Korea.

A novel strain of Gram-staining-positive actinobacterium, designated strain JR-39T, was isolated from the rhizosphere soil of bamboo (Sasa borealis) sampled in Damyang, Korea, and its taxonomic position was investigated by a polyphasic approach. The isolate formed flexuous chains of spores that were cylindrical and smooth-surfaced. Strain JR-39T grew at 4–37 °C (optimum 28 °C). The pH range for growth was pH 5–10 (optimum pH 6–8) and the NaCl range for growth was 0–5 % (w/v) with optimum growth at 1 % NaCl. The cell-wall peptidoglycan contained ll-diaminopimelic acid, glutamic acid, alanine and glycine. Whole-cell hydrolysates mainly contained glucose, mannose, ribose and rhamnose. Predominant menaquinones were MK-9 (H6), MK-9 (H8) and MK-9 (H4). The major cellular fatty acids were anteiso-C15 : 0, iso-C16 : 0, iso-C15 : 0 and iso-C14 : 0. The G+C content of the DNA was 72.3 ± 0.34 mol%. Phylogenetic analyses based on 16S rRNA gene sequence analysis indicated that strain JR-39T belonged to the genus Streptomyces, showing the highest sequence similarity to Streptomyces panaciradicis 1MR-8T (99.4 %), Streptomyces capoamus JCM 4734T (98.8 %), Streptomyces galbus DSM 40089T (98.7 %), Streptomyces longwoodensis LMG 20096T (98.7 %), Streptomyces bungoensis NBRC 15711T (98.7 %) and Streptomyces rhizophilus JR-41T (98.7 %). However, DNA–DNA hybridization assays, as well as physiological and biochemical analyses, showed that strain JR-39T could be differentiated from its closest phylogenetic relatives. On the basis of the phenotypic and genotypic characteristics, strain JR-39T represents a novel species for which the name Streptomyces sasae sp. nov. is proposed. The type strain is JR-39T ( = KACC 17182T = NBRC 109809T).
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http://dx.doi.org/10.1099/ijsem.0.000454DOI Listing
October 2015