Publications by authors named "Kun Ye"

55 Publications

Genetic characterization of Carbapenem-Resistant Escherichia coli from China, 2015-2017.

BMC Microbiol 2021 Sep 17;21(1):248. Epub 2021 Sep 17.

Laboratory Medicine Department, First Medical Center of Chinese PLA General Hospital, Beijing, 100853, China.

Background: The molecular characteristics of carbapenem-resistant Escherichia coli (CREco) remain unclear.

Methods: We conducted a multi-center bacterial resistance monitoring project from 2015 to 2017.The minimum inhibitory concentrations ofCREco were determined bybroth microdilution method. The genome sequencing of CREcoisolates was performed, and single-nucleotide polymorphism (SNP) was analyzed.

Results: A total of 144CREcoisolatescollected from 10 cities in China were involved in this study. ST167 (n = 43) is the most popular type, followed by ST410(n = 14), ST131(n = 9). There were 102 (70.83%) CREco isolates that produced various NDMs, including NDM-1 (n = 16), NDM-4(n = 1), NDM-5(n = 79), NDM-6(n = 2) and NDM-9(n = 4). In addition, 15 isolates produced KPC-2, three isolates wereIMP-4 positive, and three isolates produced OXA-48. Genetic relatedness and phylogenetic analysis showed that isolates with the same ST had a high degree of homology. Some STs (including ST167, ST410, ST131, ST46, ST405 and ST617) exhibited a trend of outbreak.

Conclusions: The majority of CREco belonged to ST167, followed by ST410 and ST131, and most of them carried various NDM-coding genes. The spread of high-risk clones of CREco has occurred in different regions of China.
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http://dx.doi.org/10.1186/s12866-021-02307-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8449468PMC
September 2021

Efficacy of acupuncture in refractory irritable bowel syndrome: study protocol for a randomised controlled trial.

BMJ Open 2021 Sep 13;11(9):e045655. Epub 2021 Sep 13.

Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, China.

Introduction: Irritable bowel syndrome (IBS) is a common chronic functional gastrointestinal disorder that presents with abdominal pain/discomfort and altered bowel patterns. IBS has multiple potential causes for which conventional medicines have had limited success, resulting in a significant number of patients who do not sensitively respond to pharmacotherapy for a period of 12 months and who develop a continuing symptom profile (described as refractory IBS) and seek help through (non)pharmacological treatments. The aim of this study is to investigate the efficacy and safety of acupuncture therapy for refractory IBS on the basis of conventional treatments.

Methods And Analysis: A total of 170 eligible patients who meet the Rome IV criteria for refractory IBS will be randomly allocated to receive acupuncture or sham acupuncture. Each patient will receive 12 sessions of acupuncture over 4 weeks and a 4-week follow-up. The primary outcome will be the IBS Symptom Severity Score. Secondary outcomes will include the proportion of participants experiencing adequate relief of global IBS symptoms, the weekly frequency of defecation, the stool properties assessed by the Bristol Grading Scale, the Work and Social Adjustment Scale, the IBS-Quality of Life score, and the Self-Rating Depression Scale and Self-Rating Anxiety Scale anxiety and depression scores. Outcome measures will be collected at baseline, 2 and 4 weeks of the intervention, and 6 and 8 weeks after the intervention. Categorical variables will be compared with Fisher's exact test or the Wilcoxon rank-sum test, and continuous variables will be compared using Student's t-test or the Wilcoxon rank-sum test.

Ethics And Dissemination: The entire project has been approved by the ethics committees of Beijing University of Chinese Medicine (2020BZYLL0507) and Sichuan Province Regional Institution for Conducting Research on Traditional Chinese Medicine (2020KL-025). The outcomes of the trial will be disseminated through peer-reviewed publications.

Trial Registration Number: NCT04276961.
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http://dx.doi.org/10.1136/bmjopen-2020-045655DOI Listing
September 2021

High-sensitivity and versatile plasmonic biosensor based on grain boundaries in polycrystalline 1L WS films.

Biosens Bioelectron 2021 Sep 3;194:113596. Epub 2021 Sep 3.

Center for High Pressure Science, State Key Lab of Metastable Materials Science and Technology, Yanshan University, Qinhuangdao, 066004, People's Republic of China.

Structural defects play an important role in exploitation of two-dimensional layered materials (2DLMs) for advanced biosensors with the increasingly high sensitivity and low detection limit. Grain boundaries (GBs), as an important type of structural defect in polycrystalline 2DLM films, potentially provide sufficient active defect sites for the immobilization of bioreceptor units via chemical functionalization. In this work, we report the selective functionalization of high-density GBs with complementary DNA receptors, via gold nanoparticle (AuNP) linkers, in wafer-scale polycrystalline monolayer (1L) W(Mo)S films as versatile plasmonic biosensing platforms. The large surface area and GB-rich nature of the polycrystalline 1L WS film enabled the immobilization of bioreceptors in high surface density with spatial uniformity, while the AuNPs perform not only as bioreceptor linkers, but also promote detection sensitivity through surface plasmon resonance enhancement effect. Therefore, the presented biosensor demonstrated highly sensitive and selective sub-femto-molar detection of representative RNA sequences from the novel coronavirus (RdRp, ORF1ad and E). This work demonstrates the immense potential of AuNP-decorated GB-rich 2DLMs in the design of ultra-sensitive biosensing platforms for the detection of biological targets beyond RNA, bringing new opportunities for novel healthcare technologies.
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http://dx.doi.org/10.1016/j.bios.2021.113596DOI Listing
September 2021

Silencing of H19 alleviates oxygen-glucose deprivation/reoxygenation-triggered injury through the regulation of the miR-1306-5p/BCL2L13 axis.

Metab Brain Dis 2021 Aug 26. Epub 2021 Aug 26.

Department of Neurosurgery, Hospital of Chengdu University of Traditional Chinese Medicine, No. 39, Shierqiao Road, Chengdu, 610072, Sichuan, China.

Cerebral ischemia/reperfusion (I/R) injury remains a leading cause of death and disability. Long noncoding RNAs (lncRNAs) exert key functions in cerebral I/R injury. Here, we sought to elucidate the mechanism underlying the regulation of H19 in cerebral I/R cell injury. An in vitro model of cerebral I/R injury was created using oxygen-glucose deprivation/reoxygenation (OGD/R). The levels of H19, miR-1306-5p and B cell lymphoma-2 (Bcl-2)-like 13 (BCL2L13) were assessed by quantitative real-time polymerase chain reaction (qRT-PCR) or western blot. Cell viability and apoptosis were determined by the Cell Counting-8 Kit (CCK-8) assay and flow cytometry, respectively. The levels of lactate dehydrogenase (LDH) and cytokines were evaluated by enzyme-linked immunosorbent assays (ELISA). Direct relationships among H19, miR-1306-5p and BCL2L13 were verified by dual-luciferase reporter, RNA immunoprecipitation (RIP) and RNA pulldown assays. Our data showed that H19 and BCL2L13 were highly expressed in the cerebral I/R injury rats and OGD/R-triggered SK-N-SH and IMR-32 cells. The knockdown of H19 or BLC2L13 alleviated OGD/R-triggered injury in SK-N-SH and IMR-32 cells. Moreover, H19 silencing protected against OGD/R-triggered cell injury by down-regulating BCL2L13. H19 acted as a sponge of miR-1306-5p and BCL2L13 was a direct target of miR-1306-5p. H19 mediated BCL2L13 expression by sequestering miR-1306-5p. Furthermore, miR-1306-5p was a molecular mediator of H19 function. These results suggested that H19 silencing alleviated OGD/R-triggered I/R injury at least partially depending on the regulation of the miR-1306-5p/BCL2L13 axis.
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http://dx.doi.org/10.1007/s11011-021-00822-4DOI Listing
August 2021

Chinese Herbal Medicine for Irritable Bowel Syndrome: A Meta-Analysis and Trial Sequential Analysis of Randomized Controlled Trials.

Front Pharmacol 2021 27;12:694741. Epub 2021 Jul 27.

School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing, China.

Chinese herbal medicine (CHM) is an important complementary and alternative therapy for the management of irritable bowel syndrome (IBS). Previous meta-analyses suggested that CHM is effective for IBS; nonetheless, its effectiveness is inconclusive owing to repeated significance testing. We aimed to examine the efficacy and safety of CHM for IBS through a meta-analysis and trial sequential analysis (TSA). We searched OVID Medline, Embase, Cochrane Central Register of Controlled Trials, and Web of Science from January 1, 1980, to September 20, 2020. The primary outcome was adequate relief of global IBS symptoms. The secondary outcomes included relief of abdominal pain and treatment-related adverse events. The relative ratio (RR) and required information size (RIS) were calculated for each outcome. Ten trials recruiting 2,501 participants were included. Seven (70%) trials were at low risk of bias (RoB). Compared with placebo, CHM was associated with a significantly higher proportion of adequate relief of global IBS symptoms [RR 1.76 (95% confidence interval (95%CI), 1.33-2.33); = 81.1%; < 0.001]. The RIS was 1,083 for the primary outcome, and the accrued information size was 1,716. The analysis of the relief of abdominal pain (three trials with 916 participants) showed similar results compared with placebo [RR 1.85 (95%CI, 1.59-2.14); = 0%; < 0.001; RIS = 197 participants]. CHM was associated with a higher proportion of adverse events compared with placebo [RR 1.51 (95%CI, 1.14-2); = 0%; = 0.004]. CHM was effective in relieving IBS symptoms but caused a higher adverse event rate than placebo. TSA analysis confirmed the findings with sufficient information size.
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http://dx.doi.org/10.3389/fphar.2021.694741DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8353248PMC
July 2021

Genetic characteristics of OXA-48-producing Enterobacterales from China.

J Glob Antimicrob Resist 2021 Sep 18;26:285-291. Epub 2021 Jul 18.

Department of Laboratory Medicine, First Medical Center of Chinese PLA General Hospital, Beijing 100853, China. Electronic address:

Objectives: In China, OXA-48-producing Enterobacterales have been identified sporadically, causing small-scale regional outbreaks. This study investigated the molecular epidemiology, transmission and evolution of OXA-48-producing Enterobacterales and pOXA48 from mainland China.

Methods: We conducted a multicentre resistance monitoring project from 2013-2018. Genome sequencing of OXA-48-produicng isolates was performed. SNPs were analysed. Eleven isolates were selected for subsequent SMRT sequencing. Genome sequences were annotated, and alignment of the complete sequences of bla-carrying plasmids from a subset of isolates that underwent long-read sequencing was performed.

Results: In total, 41 OXA-48-producing Enterobacterales were included in this study (34 Klebsiella pneumoniae, 3 Escherichia coli, 3 Enterobacter cloacae complex and 1 Klebsiella oxytoca). OXA-48-produicng K. pneumoniae (OXAKp) ST383, ST147 and ST11 caused outbreaks of different scales in our hospital. OXA-48-producing E. coli ST156 and ST648, E. cloacae complex ST414 and ST418, and K. oxytoca ST34 were also identified. bla was embedded in a Tn1999.2 structure located in IncL plasmids with different sizes (63.58-109.14 kb). Importantly, K. pneumoniae ST11 co-producing KPC-2 and OXA-48 was identified in our hospital and it is possible that KPC-2-producing K. pneumoniae ST11 obtained the bla-carrying plasmid during its spread. A novel 109-kb bla-carrying IncL plasmid was identified from OXAKp ST11. Possibly, two plasmids in OXAKp ST383 were integrated to form this larger plasmid.

Conclusion: OXA-48-produicng Enterobacterales were sporadic in China. Importantly, K. pneumoniae ST11 co-producing KPC-2 and OXA-48 has emerged causing an outbreak in China. This high-risk multidrug-resistant clone exhibited high compatibility and strong integration ability with foreign resistance plasmids.
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http://dx.doi.org/10.1016/j.jgar.2021.07.006DOI Listing
September 2021

Proteomics and metabonomics analyses of Covid-19 complications in patients with pulmonary fibrosis.

Sci Rep 2021 07 16;11(1):14601. Epub 2021 Jul 16.

Department of Endocrinology, the People's Hospital of Guangxi Zhuang Autonomous Region, 6 Taoyuan Road, Nanning, 530021, Guangxi, People's Republic of China.

Pulmonary fibrosis is a devastating disease, and the pathogenesis of this disease is not completely clear. Here, the medical records of 85 Covid-19 cases were collected, among which fibrosis and progression of fibrosis were analyzed in detail. Next, data independent acquisition (DIA) quantification proteomics and untargeted metabolomics were used to screen disease-related signaling pathways through clustering and enrichment analysis of the differential expression of proteins and metabolites. The main imaging features were lesions located in the bilateral lower lobes and involvement in five lobes. The closed association pathways were FcγR-mediated phagocytosis, PPAR signaling, TRP-inflammatory pathways, and the urea cycle. Our results provide evidence for the detection of serum biomarkers and targeted therapy in patients with Covid-19.
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http://dx.doi.org/10.1038/s41598-021-94256-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8285535PMC
July 2021

Target-induced synergetic modulation of electrochemical tag concentration and electrode surface passivation for one-step sampling filtration-free detection of acid phosphatase activity.

Talanta 2021 Oct 11;233:122500. Epub 2021 May 11.

Institute of Green Chemistry and Chemical Technology, School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang, 212013, China. Electronic address:

As a biomarker of several diseases, the activity of acid phosphatase (ACP) is generally used to assistantly diagnose these diseases. Thus, developing reliable ACP activity analytical methods becomes quite significant. Herein, we recommend a one-step sampling filtration-free electrochemical method for ACP activity determination based on the target-induced synergetic modulation of tag concentration and surface passivation. MnO microspheres with favorable oxidase-mimicking activity are synthesized to catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) to its product TMBox, resulting in a remarkable re-reduction signal of TMBox to TMB recorded by an integrated electrochemical system consisting of screen-printed electrode (SPE) and 3D-printed holder. When hexametaphosphate ions (HMPi) with rich negative charges are employed to interact positively charged TMBox, the formed flocculent precipitate TMBox-HMPi automatically sedimentates onto SPE surface, and both the decreased concentration of free TMBox in solution and the increased electrode surface passivation triggered by TMBox-HMPi sedimentation synergistically reduce the re-reduction signal of TMBox. When ACP is present, it hydrolyzes the HMPi substrate, greatly relieving the formation of the TMBox-HMPi precipitate and its sedimentation onto SPE surface. As a result, the electrochemical re-reduction signal of TMBox becomes remarkable again. With the strategy of using one stimulus to generate two-fold signal change, highly sensitive ACP activity detection was realized, with a wide linear range from 0.05 to 50 U/L and a limit of detection down to 0.024 U/L. Reliable monitoring of ACP activity in clinical serum was also demonstrated.
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http://dx.doi.org/10.1016/j.talanta.2021.122500DOI Listing
October 2021

Grain-boundary-rich polycrystalline monolayer WS film for attomolar-level Hg sensors.

Nat Commun 2021 Jun 23;12(1):3870. Epub 2021 Jun 23.

Center for High Pressure Science, State Key Lab of Metastable Materials Science and Technology, Yanshan University, Qinhuangdao, People's Republic of China.

Emerging two-dimensional (2D) layered materials have been attracting great attention as sensing materials for next-generation high-performance biological and chemical sensors. The sensor performance of 2D materials is strongly dependent on the structural defects as indispensable active sites for analyte adsorption. However, controllable defect engineering in 2D materials is still challenging. In the present work, we propose exploitation of controllably grown polycrystalline films of 2D layered materials with high-density grain boundaries (GBs) for design of ultra-sensitive ion sensors, where abundant structural defects on GBs act as favorable active sites for ion adsorption. As a proof-of-concept, our fabricated surface plasmon resonance sensors with GB-rich polycrystalline monolayer WS films have exhibited high selectivity and superior attomolar-level sensitivity in Hg detection owing to high-density GBs. This work provides a promising avenue for design of ultra-sensitive sensors based on GB-rich 2D layered materials.
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http://dx.doi.org/10.1038/s41467-021-24254-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8222231PMC
June 2021

GABA Production by Human Intestinal spp.: Prevalence, Regulation, and Role in Acid Stress Tolerance.

Front Microbiol 2021 15;12:656895. Epub 2021 Apr 15.

Laboratory of Food Biotechnology, Department of Health Sciences and Technology, ETH Zürich, Zürich, Switzerland.

The high neuroactive potential of metabolites produced by gut microbes has gained traction over the last few years, with metagenomic-based studies suggesting an important role of microbiota-derived γ-aminobutyric acid (GABA) in modulating mental health. Emerging evidence has revealed the presence of the glutamate decarboxylase (GAD)-encoding gene, a key enzyme to produce GABA, in the prominent human intestinal genus . Here, we investigated GABA production by in culture and metabolic assays combined with comparative genomics and phylogenetics. A total of 961 genomes were analyzed and 17 metabolically and genetically diverse human intestinal isolates representing 11 species were screened . Using the model organism DSM 2079, we determined GABA production kinetics, its impact on milieu pH, and we assessed its role in mitigating acid-induced cellular damage. We showed that the GAD-system consists of at least four highly conserved genes encoding a GAD, a glutaminase, a glutamate/GABA antiporter, and a potassium channel. We demonstrated a high prevalence of the GAD-system among with 90% of all genomes (96% in human gut isolates only) harboring all genes of the GAD-system and 16 intestinal strains producing GABA (ranging from 0.09 to 60.84 mM). We identified glutamate and glutamine as precursors of GABA production, showed that the production is regulated by pH, and that the GAD-system acts as a protective mechanism against acid stress in , mitigating cell death and preserving metabolic activity. Our data also indicate that the GAD-system might represent the only amino acid-dependent acid tolerance system in . Altogether, our results suggest an important contribution of in the regulation of the GABAergic system in the human gut.
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http://dx.doi.org/10.3389/fmicb.2021.656895DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8082179PMC
April 2021

Xylitol enhances synthesis of propionate in the colon via cross-feeding of gut microbiota.

Microbiome 2021 03 18;9(1):62. Epub 2021 Mar 18.

School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou, 310018, China.

Background: Xylitol, a white or transparent polyol or sugar alcohol, is digestible by colonic microorganisms and promotes the proliferation of beneficial bacteria and the production of short-chain fatty acids (SCFAs), but the mechanism underlying these effects remains unknown. We studied mice fed with 0%, 2% (2.17 g/kg/day), or 5% (5.42 g/kg/day) (weight/weight) xylitol in their chow for 3 months. In addition to the in vivo digestion experiments in mice, 3% (weight/volume) (0.27 g/kg/day for a human being) xylitol was added to a colon simulation system (CDMN) for 7 days. We performed 16S rRNA sequencing, beneficial metabolism biomarker quantification, metabolome, and metatranscriptome analyses to investigate the prebiotic mechanism of xylitol. The representative bacteria related to xylitol digestion were selected for single cultivation and co-culture of two and three bacteria to explore the microbial digestion and utilization of xylitol in media with glucose, xylitol, mixed carbon sources, or no-carbon sources. Besides, the mechanisms underlying the shift in the microbial composition and SCFAs were explored in molecular contexts.

Results: In both in vivo and in vitro experiments, we found that xylitol did not significantly influence the structure of the gut microbiome. However, it increased all SCFAs, especially propionate in the lumen and butyrate in the mucosa, with a shift in its corresponding bacteria in vitro. Cross-feeding, a relationship in which one organism consumes metabolites excreted by the other, was observed among Lactobacillus reuteri, Bacteroides fragilis, and Escherichia coli in the utilization of xylitol. At the molecular level, we revealed that xylitol dehydrogenase (EC 1.1.1.14), xylulokinase (EC 2.7.1.17), and xylulose phosphate isomerase (EC 5.1.3.1) were key enzymes in xylitol metabolism and were present in Bacteroides and Lachnospiraceae. Therefore, they are considered keystone bacteria in xylitol digestion. Also, xylitol affected the metabolic pathway of propionate, significantly promoting the transcription of phosphate acetyltransferase (EC 2.3.1.8) in Bifidobacterium and increasing the production of propionate.

Conclusions: Our results revealed that those key enzymes for xylitol digestion from different bacteria can together support the growth of micro-ecology, but they also enhanced the concentration of propionate, which lowered pH to restrict relative amounts of Escherichia and Staphylococcus. Based on the cross-feeding and competition among those bacteria, xylitol can dynamically balance proportions of the gut microbiome to promote enzymes related to xylitol metabolism and SCFAs. Video Abstract.
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http://dx.doi.org/10.1186/s40168-021-01029-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7977168PMC
March 2021

Comparison of three species of Elizabethkingia genus by whole-genome sequence analysis.

FEMS Microbiol Lett 2021 04;368(5)

Center of Laboratory Medicine, the First Medical Center of Chinese PLA General Hospital, 28 Fu Xing Road, Beijing 100853, China.

Elizabethkingia are found to cause severe neonatal meningitis, nosocomial pneumonia, endocarditis and bacteremia. However, there are few studies on Elizabethkingia genus by comparative genomic analysis. In this study, three species of Elizabethkingia were found: E. meningoseptica, E. anophelis and E. miricola. Resistance genes and associated proteins of seven classes of antibiotics including beta-lactams, aminoglycosides, macrolides, tetracyclines, quinolones, sulfonamides and glycopeptides, as well as multidrug resistance efflux pumps were identified from 20 clinical isolates of Elizabethkingia by whole-genome sequence. Genotype and phenotype displayed a good consistency in beta-lactams, aminoglycosides and glycopeptides, while contradictions exhibited in tetracyclines, quinolones and sulfonamides. Virulence factors and associated genes such as hsp60 (htpB), exopolysaccharide (EPS) (galE/pgi), Mg2+ transport (mgtB/mgtE) and catalase (katA/katG) existed in all clinical and reference strains. The functional analysis of the clusters of orthologous groups indicated that 'metabolism' occupied the largest part in core genome, 'information storage and processing' was the largest group in both accessory genome and unique genome. Abundant mobile elements were identified in E. meningoseptica and E. anophelis. The most significant finding in our study was that a single clone of E. anophelis had been circulating within diversities of departments in a clinical setting for nearly 18 months.
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http://dx.doi.org/10.1093/femsle/fnab018DOI Listing
April 2021

Vitamin B12 Enriched in Spinach and its Effects on Gut Microbiota.

J Agric Food Chem 2021 Feb 9;69(7):2204-2212. Epub 2021 Feb 9.

School of Food Science and Bioengineering, Zhejiang Gongshang University, Hangzhou 310018, China.

Meat and fermented foods are the main source of vitamin B12 (cobalamin) for human beings. Therefore, daily cobalamin intake is a big problem for vegans. In this study, cyanocobalamin (CNCBL) was added to the culture broth for cobalamin enrichment in spinach. After 36 h of cultivation, the accumulated CNCBL in the spinach leaves (wet weight) was as high as 0.48% (concentration), and the leaves still contained 0.94 ± 0.11 μg/g CNCBL after boiling, which could provide consumer daily requirement of CNCBL. Because CNCBL supplementation had adverse effects on gut microbiota, this study focused on the effect of the combination of spinach and CNCBL on gut microbiota as well. After the boiled leaves were passed through an gastrointestinal tract simulation system, it was found that the spinach protected CNCBL against the low-pH gastric acid. Moreover, compared with the CNCBL supplement group, the relative abundances of and increased, and the relative abundance of Proteobacteria, especially spp., reduced. Analysis of short-chain fatty acids (SCFAs) showed that cobalamin-rich spinach was positively correlated with , propionate, and butyrate. The results showed that the method of enriching spinach with CNCBL was effective and had beneficial effects on gut microbiota and SCFAs.
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http://dx.doi.org/10.1021/acs.jafc.0c07597DOI Listing
February 2021

One-pot construction of acid phosphatase and hemin loaded multifunctional metal-organic framework nanosheets for ratiometric fluorescent arsenate sensing.

J Hazard Mater 2021 06 29;412:124407. Epub 2020 Oct 29.

Institute of Green Chemistry and Chemical Technology, School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, China. Electronic address:

Exploring high-performance sensors for toxic arsenic detection is highly desired because of its great threat to the environment. Herein, we report a ratiometric fluorescent biosensor based on acid phosphatase and hemin loaded multifunctional Zn-based metal-organic framework (ACP/[email protected]) for high-performance arsenate (As(Ⅴ)) sensing. ACP/[email protected] is constructed by self-assembly, where hemin exhibits peroxidase-like activity and 2-aminoterephthalic acid ligand endows ACP/[email protected] with an intrinsic fluorescence (452 nm). When ACP/[email protected] catalyzes the oxidation of o-phenylenediamine (OPD), fluorescent 2,3-diaminophenazine (DAP) with an emission signal (564 nm) is produced and weakens ACP/[email protected] intrinsic fluorescence (452 nm) due to inner filter effect; after adding ascorbic acid 2-phosphate (AAP), ACP can hydrolyze AAP and produce ascorbic acid, which competitively suppresses the oxidation of OPD, resulting in the decrease of DAP signal (564 nm) and the recovery of ACP/[email protected] signal (452 nm); when As(V) is added, it irreversibly poisons ACP against hydrolyzing AAP, and the fluorescence signal at 564 nm recovers and the one at 452 nm is suppressed again. High-sensitivity and high-selectivity detection of As(V) (3.33-300 μg L) is realized, with a detection limit of 1.05 μg L. The biosensor was also successfully employed to detect total arsenic and As(V) in rice.
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http://dx.doi.org/10.1016/j.jhazmat.2020.124407DOI Listing
June 2021

Histopathological substrate of the atrial myocardium in the progression of obstructive sleep apnoea-related atrial fibrillation.

Sleep Breath 2021 Jun 7;25(2):807-818. Epub 2021 Jan 7.

Xinjiang Key Laboratory of Cardiac Electrophysiology and Cardiac Remodeling, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China.

Background: Obstructive sleep apnoea (OSA) is closely related to atrial fibrillation (AF), and OSA-induced atrial structural remodelling is the basis of AF maintenance. However, the process of atrial structural remodelling during the progression of acute OSA to chronic OSA is still unclear.

Objective: To investigate changes in the atrial myocardium in acute sleep apnoea (6 h) and chronic sleep apnoea (12 weeks) by echocardiography, atrial myocardium morphology analysis, PAS staining, TUNEL staining, Masson's trichrome staining and analyses of ultrastructural changes.

Methods: Eighteen adult beagle dogs under general anaesthesia were used to establish an OSA model. The animals were divided into the control group, acute OSA group and chronic OSA group, and there were six animals in each group. Cardiac ultrasounds of dogs from the three groups were examined. Left and right atrial muscle tissues were taken for HE staining, PAS staining, TUNEL staining, Masson's trichrome staining and transmission electron microscopy.

Results: In the acute OSA model, the left atrial diameter of the dogs began to increase 3 h after ventilation, and this difference was more obvious at 6 h. The morphology of the myocardial cells did not change significantly, but mitochondrial swelling was observed in some atrial myocytes at 3 h. In the chronic OSA model, the left atrial diameter gradually increased, the volume of the right and left atria increased, the glycogen and collagen volume fractions and apoptosis ratio were significantly increased in atrial myocytes, mitochondria swelling and lengthening occurred in some atrial myocytes, the matrix became lighter, the mitochondrial ridge density decreased and the myofilament arrangement was disordered. The disc was distorted and not continuous, and there was some cardiomyocyte necrosis.

Conclusion: With the prolongation of apnoea, the atrium gradually enlarges, myocardial cells become disordered, glycogen aggregates, the number of necrotic cells increases, fibrosis worsens, mitochondrial abnormalities occur and the arrangement of the discs are disordered, providing a basis for the maintenance of AF.
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http://dx.doi.org/10.1007/s11325-020-02128-8DOI Listing
June 2021

Gut microbiota affect the formation of calcium oxalate renal calculi caused by high daily tea consumption.

Appl Microbiol Biotechnol 2021 Jan 6;105(2):789-802. Epub 2021 Jan 6.

Shandong Zhongke-jiayi Bioengineering Co., Ltd, Weifang, People's Republic of China.

Kidney stones are a common and frequently occurring disease worldwide. Stones can cause urinary tract obstruction, pain, haematuria, and other symptoms. In this study, the relationship between calcium oxalate renal calculi and gut microbiota was considered. The dietary habits of 30 patients with calcium oxalate kidney stones and 30 healthy people were investigated. The 16S rDNA sequences and short-chain fatty acids (SCFAs) in their stool samples were analysed. We identified 5 genera of the gut microbiota as biomarkers for calcium oxalate renal calculi, namely, Bacteroides, Phascolarctobacterium, Faecalibacterium, Akkermansia, and Lactobacillus, with a receiver operating characteristic (ROC) curve value of 0.871 (95% confidence interval (CI) 0.785-0.957). Phascolarctobacterium and Faecalibacterium showed a positive relationship with SCFA synthesis to reduce the risk of kidney stones. Meanwhile, according to the analysis, Lactobacillus spp. made the largest contribution (79%) to prevent kidney stones caused by tea consumption, since tea offers the great parts of oxalate in kidney stone formation. Three strains of Lactobacillus spp. were isolated from stools of a healthy person with a high level of tea consumption who did not suffer from kidney stones. All these strains survived in the colon with supplementation of high concentrations of tea and efficiently degraded oxalic acid (Ca. 50%) in an in vitro colonic simulation. Therefore, a suitable adjustment of the gut microbiota or SCFA concentration enhanced the degradation of oxalate from food, which can be applied to prevent the formation of calcium oxalate renal calculi caused by tea. KEY POINTS: • Five genera, including Lactobacillus, were identified as biomarkers for calcium oxalate renal calculi. • Lactobacillus is a potential gut bacterium associated with preventing kidney stone formation. • Isolated Lactobacillus strains have the ability to degrade oxalic acid in vitro.
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http://dx.doi.org/10.1007/s00253-020-11086-wDOI Listing
January 2021

Does Serum Vitamin D Level Affect COVID-19 Infection and Its Severity?-A Case-Control Study.

J Am Coll Nutr 2020 Oct 13:1-8. Epub 2020 Oct 13.

Department of Hepatobiliary Surgery, The People's Hospital of Guangxi Zhuang Autonomous Region, Nanning, China.

Background: As effective medication to treat COVID-19 is currently unavailable, preventive remedies may be particularly important.

Objective: To examine the relationship between serum 25-hydroxy vitamin D (25(OH)D) level and COVID-19 infection, its severity, and its clinical case characteristics.

Methods: This case-control study compared serum 25(OH)D levels and rates of vitamin D deficiency (VDD) between 80 healthy controls and 62 patients diagnosed with COVID-19 and admitted to Guangxi People's Hospital, China, 2/16/2020-3/16/2020. Cases were categorized into asymptomatic, mild/moderate, and severe/critical disease. Logistic regression analysis was conducted to examine the associations between 25(OH)D level, or VDD, and case status/severity of COVID-19 while controlling for demographics and comorbidities. A threshold level of vitamin D for conveying COVID-19 risk was estimated.

Results: Severe/critical COVID-19 cases were significantly older and had higher percentages of comorbidity (renal failure) compared to mild cases. The serum 25(OH)D concentration in COVID-19 patient was much lower than that in healthy control. And 25(OH)D level was the lowest in severe/critical cases, compared with mild cases. In further, significantly higher rates of VDD were found in COVID-19 cases (41.9%) compared to healthy controls (11.1%). And VDD was the greatest in severe/critical cases (80%), compared with mild cases (36%). These statistically significant associations remained even after controlling for demographics and comorbidities. A potential threshold of 25(OH)D (41.19 nmol/L) to protect against COVID-19 was identified.

Conclusion: Elderly and people with comorbidities were susceptible to severe COVID-19 infection. VDD was a risk factor for COVID-19, especially for severe/critical cases. While further confirmation is needed, vitamin D supplementation may have prevention or treatment potential for COVID-19 disease.
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http://dx.doi.org/10.1080/07315724.2020.1826005DOI Listing
October 2020

Tri-functional Fe-Zr bi-metal-organic frameworks enable high-performance phosphate ion ratiometric fluorescent detection.

Nanoscale 2020 Oct;12(37):19383-19389

School of Mechanical and Materials Engineering, Washington State University, Pullman, WA 99164, USA.

Metal-organic frameworks (MOFs) featured with flexible design and versatile properties are finding increasing applications. In particular, integrating multiple functions into one framework can bring them improved detection efficiency towards various analytes. Herein, for the first time, a Fe-Zr bi-metal-organic framework (UiO-66(Fe/Zr)-NH2) with three functions (intrinsic fluorescence, peroxidase-mimicking activity, and specific recognition) is designed to establish a ratiometric fluorescent platform for high-performance phosphate ion (Pi) sensing. The use of a fluorescent organic ligand endows the MOF material with a strong intrinsic fluorescence at 435 nm. The presence of Fe3+/Fe2+ nodes offers good enzyme-like capacity to catalyze the o-phenylenediamine (OPD) substrate to fluorescent OPDox (555 nm), which then quenches the intrinsic fluorescence of UiO-66(Fe/Zr)-NH2 due to the inner filter effect. The Zr4+ nodes in the MOF material act as selective sites for Pi recognition. When Pi exists, it specifically adsorbs onto UiO-66(Fe/Zr)-NH2 and decreases the latter's peroxidase-mimetic activity, resulting in the less production of fluorescent OPDox. As a consequence, the intrinsic fluorescence of UiO-66(Fe/Zr)-NH2 at 435 nm is restored, and the signal from OPDox at 555 nm is reduced inversely. With the ratiometric strategy, efficient determination of Pi with outstanding sensitivity and selectivity was realized, giving a detection limit down to 85 nM in the concentration range of 0.2-266.7 μM. Accurate measurement of the target in practical water matrices was also validated, indicating its promising application for Pi analysis in environmental and other fields.
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http://dx.doi.org/10.1039/d0nr04531dDOI Listing
October 2020

Combining CeVO oxidase-mimetic catalysis with hexametaphosphate ion induced electrostatic aggregation for photometric sensing of alkaline phosphatase activity.

Anal Chim Acta 2020 Aug 18;1126:16-23. Epub 2020 Jun 18.

School of Environmental and Chemical Engineering, Jiangsu University of Science and Technology, Zhenjiang, 212003, PR China.

In the present work, a novel alkaline phosphatase (ALP) activity colorimetric assay is proposed by integrating the oxidase-mimicking catalytic characteristic of CeVO nanoparticles with the hexametaphosphate ion (HMPi) mediated electrostatic aggregation. The CeVO nanoparticles exhibit good oxidase-mimetic catalytic ability to promote the 3,3',5,5'-tetramethylbenzidine (TMB) oxidation to TMBox, offering a significant change from colorless to blue. After a small amount of HMPi is added, the strong electrostatic interaction between the negatively charged HMPi species and the positively charged TMBox product leads to the aggregation of the latter, generating an aubergine HMPi-TMBox agglomerate. After the agglomerate is filtered out, the reaction solution turns to be almost colorless. When ALP is used to hydrolyze the HMPi species in advance, the electrostatic aggregation process is remarkably restrained, thus retaining the blue color of the CeVO catalyzed TMB solution. According to the new sensing strategy, highly selective and sensitive analysis of ALP activity was realized, providing a wide detection range from 1 to 210 U/L and a detection limit of 0.68 U/L. Accurate measurement of ALP activity in clinical serum matrices was also validated, demonstrating the practicability of the proposed assay in practical applications.
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http://dx.doi.org/10.1016/j.aca.2020.06.024DOI Listing
August 2020

Smartphone-assisted off─on photometric determination of phosphate ion based on target-promoted peroxidase-mimetic activity of porous CeZrO (x≥0.5) nanocomposites.

Environ Res 2020 10 12;189:109921. Epub 2020 Jul 12.

Institute of Green Chemistry and Chemical Technology, School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang, 212013, PR China. Electronic address:

Given the level of phosphate ion (Pi) is a significant indicator of eutrophication in environmental waters, it becomes quite important to develop efficient methods for its monitoring. In this research, we developed a smartphone-assisted off─on photometric approach for Pi analysis based on the analyte-promoted peroxidase-mimicking catalytic activity of porous CeZrO (x ≥ 0.5) nanocomposites. The Ce/Ce redox pair in CeZrO endowed it with certain activity to catalyze the 3,3',5,5'-tetramethylbenzidine (TMB) color reaction with the participation of HO, and both the existing Zr and Ce species enabled the nanozyme to specifically recognize Pi. It was observed that the bonded Pi could greatly promote the peroxidase-like activity of the CeZrO nanocomposite towards positively charged TMB. According to the new finding, high-performance sensing of Pi with wide detection range, high sensitivity and good selectivity was realized, giving a detection limit down to 0.09 μM. Further, a 3D-printed smartphone-based signal reading system was designed and coupled with the sensing method, enabling the rapid, convenient, in-field and instrument-free analysis of Pi for environmental monitoring.
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http://dx.doi.org/10.1016/j.envres.2020.109921DOI Listing
October 2020

A novel alkaline phosphatase activity sensing strategy combining enhanced peroxidase-mimetic feature of sulfuration-engineered CoO with electrostatic aggregation.

Anal Bioanal Chem 2020 Sep 16;412(23):5551-5561. Epub 2020 Jul 16.

School of Environmental and Chemical Engineering, Jiangsu University of Science and Technology, Zhenjiang, 212003, Jiangsu, China.

Given alkaline phosphatase (ALP) takes part in the phosphorylation/dephosphorylation processes in the body, its activity is universally taken as an important indicator of many diseases, and thus developing reliable and efficient methods for ALP activity determination becomes quite important. Here, we propose a new sensing strategy for ALP activity by integrating the improved peroxidase-mimicking catalysis of sulfuration-engineered CoO with the hexametaphosphate ion (HMPi)-mediated electrostatic aggregation. After sulfuration engineering, the CoO composite coming from the pyrolysis of ZIF-67 exhibits enhanced peroxidase-mimetic catalytic ability to oxidize 3,3',5,5'-tetramethylbenzidine (TMB) to its oxide TMBox, offering a remarkable color change from colorless to mazarine; with the presence of HMPi, the rapid electrostatic assembly of negatively charged HMPi and positively charged TMBox leads to the aggregation of the latter, resulting in a color fading phenomenon; when ALP is added in advance to hydrolyze the HMPi mediator, the aggregation procedure is significantly suppressed, and such that the solution color can be recovered. Based on this principle, efficient determination of ALP activity was gained, giving a wide detection scope from 0.8 to 320 U/L and a detection limit as low as 0.38 U/L. Reliable analysis of the target in serum samples was also achieved, verifying the feasibility and practicability of our strategy in measuring ALP activity for clinical applications. Graphical abstract.
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http://dx.doi.org/10.1007/s00216-020-02815-1DOI Listing
September 2020

CT features of COVID-19 patients with two consecutive negative RT-PCR tests after treatment.

Sci Rep 2020 07 14;10(1):11548. Epub 2020 Jul 14.

Department of Ophthalmology, People's Hospital of Guangxi Zhuang Autonomous Region, Nanning, China.

The objective of this study is to expound the CT features of COVID-19 patients whose throat swab samples were negative for two consecutive nucleic acid tests after treatment. We retrospectively reviewed 46 COVID-19 patients with two consecutive negative RT-PCR tests after treatment. The cases were divided into moderate group and severe/critical group according to disease severity. Clinical and CT scanning data were collected. CT signs of pulmonary lesions and the score of lung involvement were expounded. Thirty-nine moderate cases and seven severe/critical cases were included. Residual pulmonary lesions were visible in CT images. Moderate patients showed peripheral lesions while severe/critical cases exhibited both central and peripheral lesions with all lobes involvement. Mixed ground glass opacity (GGO) and pulmonary consolidation were noted. A larger proportion of severe patients showed reticular pulmonary interstitium thickening. Air bronchogram, pleural effusion, vascular enlargement, bronchial wall thickening, bronchiectasis, pleural thickening and pleural adhesion were more frequently observed in severe/critical group. The severe/critical group showed higher CT score. Pulmonary lesions persisted even after twice consecutive negative nucleic acid tests. We strongly recommended regular follow-up of CT scans after nucleic acid tests conversion. Evaluation of complete remission should base on chest CT.
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http://dx.doi.org/10.1038/s41598-020-68509-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7360570PMC
July 2020

Modified transperitoneal versus retroperitoneal laparoscopic radical nephroureterectomy in the management of upper urinary tract urothelial carcinoma: Best practice in a single center with updated results.

J Int Med Res 2020 Jun;48(6):300060520928788

Department of Urology, The Second Xiangya Hospital, Central South University, Changsha, Hunan, China.

Objective: Radical nephroureterectomy remains the gold standard for the surgical treatment of upper urinary tract urothelial carcinoma (UTUC). Based on previous research, we prospectively compared the advantages of transperitoneal laparoscopic radical nephroureterectomy (TLNU) with a three-port technique in a single position versus retroperitoneal laparoscopic radical nephroureterectomy (RLNU).

Methods: We evaluated 48 patients diagnosed with UTUC at our institution from January 2015 to October 2019. The patients underwent either TLNU (n = 24) or RLNU (n = 24). We randomly assigned the patients to each technique group based on their body mass index because our experience has shown that the body mass index is the main interfering factor for this surgery. The baseline characteristics and perioperative outcomes were compared between the groups.

Results: We found no significant differences in the baseline characteristics, time until recovery of intestinal function, or postoperative hospital stay between the two groups. However, the TLNU group had a shorter operation time and better postoperative pain control than the RLNU group.

Conclusion: Modified TLNU is associated with a shorter operative time and less severe postoperative pain compared with RLNU. Both techniques are safe and reliable with adequate management, and their therapeutic effects are comparable in other aspects.
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http://dx.doi.org/10.1177/0300060520928788DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7297496PMC
June 2020

Characterization of a multidrug-resistant Klebsiella pneumoniae ST3330 clone responsible for a nosocomial outbreak in a neonatal intensive care unit.

Ann Palliat Med 2020 May 14;9(3):1092-1102. Epub 2020 May 14.

Medical School of Chinese PLA & Department of Clinical Laboratory, Medical Laboratory Center, Chinese PLA General Hospital, Beijing 100853, China.

Background: The incidence of Klebsiella pneumonia (Kp), which has often been found to produce, extended spectrum beta-lactamase (ESBL), is rising rapidly and poses a serious risk to neonates. To date, the mechanisms related to the spread of ESBL-Kp have not been fully elucidated. This study aimed to investigate the phenotypes, genotypes, and genetic relatedness of ESBL-KP that caused an outbreak of sepsis among neonates in an intensive care unit of a Beijing hospital.

Methods: Between April 2016 and May 2018, 21 non-repetitive clinical ESBL-Kp isolates were collected from a neonatal intensive care unit (NICU) in Beijing, China and were retrospectively analyzed. Pulsed-field gel electrophoresis (PFGE) was used to analyze genetic relatedness, a VITEK 2 AST test kit was used to test antimicrobial susceptibility, sequence type (ST) was analyzed through multilocus sequence typing (MLST), and resistance genes were identified by PCR. Virulence gene profiles, biofilm formation assay, and serum killing assay were used for virulence-associated determinants.

Results: All strains expressed the same antibiotype, combining ESBL production, third generation cephalosporins resistance and carbapenems sensitive. Sixteen of them produced β-lactamases (CTX-M-3 and TEM-1B), while others possessed CTX-M-15, CTX-M-24, CTX-M-66, TEM-1C, SHV-26, SHV172, and OXA-1. PFGE confirmed 5 types (A, B, C, D and E) and MLST identified a ST3330 clone (16 strains), a ST2791 clone (2 strains), a ST37 clone (1 strain), a ST34 clone (1 strain), and a ST2740 clone (1 strain). PFGE type A strains, which belong to ST3330, were identified as the main pathogens involved in the outbreak. All isolates contained virulence genes iutA and mrk. PFGE type A carried both mrk (type 3 fimbriae, biofilm formation) and fimH (type 1 fimbriae), and other STs possessed mrk. Isolates belonging to the endemic ST3330 lineage produced more biofilm than other ST isolates (median OD590 1.829 vs. 0.2280, respectively; P<0.0001). All five PFGE types isolates showed serum high sensitivity (grade 1).

Conclusions: The dissemination and outbreak of ESBL-producing K. pneumoniae in this study seemed to be clonal, and the outbreak was mainly caused by ST3330 K. pneumoniae. The detection of genes (mrk and fimH) belonging to the biofilm formation may partly explain the epidemic strain has high colonization and diffusion potential.
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http://dx.doi.org/10.21037/apm-20-958DOI Listing
May 2020

Analysis of DNA methylation differences in gonads of the large yellow croaker.

Gene 2020 Jul 4;749:144754. Epub 2020 May 4.

Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural Affairs, Jimei University, Xiamen 361021, China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266235, China. Electronic address:

DNA methylation is an essential epigenetic modification that significantly regulates gene expression during development and differentiation. In this study, genome-wide methylation analysis of different gonads of the large yellow croaker was performed using whole-genome bisulfite sequencing (WGBS), which has characterized DNA methylation patterns in gonad tissue and identified candidate regions for future studies. Clustering analysis revealed that male and neomale methylation patterns were close compared to female. Based on KEGG pathway analysis of differentially methylated genes, we obtained signaling pathways related to gonadal development. We further investigated the methylation status of previously reported sex determination genes, and found that these genes showed different methylation status in three types of gonads, which may provide important clues to reveal the sex determination genes in the large yellow croaker. Furthermore, combined with transcriptome analysis, we found 7 sex-related genes in three comparison groups where expression negatively correlated with methylation.
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http://dx.doi.org/10.1016/j.gene.2020.144754DOI Listing
July 2020

Correlation between antibiotic use in childhood and subsequent inflammatory bowel disease: a systematic review and meta-analysis.

Scand J Gastroenterol 2020 Mar 17;55(3):301-311. Epub 2020 Mar 17.

Department of Gastroenterology, First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, China.

Antibiotic use leads to a cascade of inflammatory reaction in the gastrointestinal tract due to its association with a temporary disruption of human microbiome. To explore the undetermined correlation between antibiotic use in childhood and subsequent inflammatory bowel disease (IBD). PUBMED, EMBASE and Cochrane Central Register of Controlled Trials were searched to identify related articles. We extracted and pooled the (adjusted) odds ratio (OR) and (adjusted) risk ratio (RR). This systematic review and meta-analysis included 11 studies. The pooled OR of all 11 studies was 1.5 (95% confidence interval (CI): 1.22-1.85). The pooled ORs of the subsequent Crohn's disease and ulcerative colitis after antibiotic use in childhood were 1.59 (95% CI: 1.06-2.4) and 1.22 (95% CI: 0.82-1.8). The sensitivity analysis showed no change. The meta-regression showed there was not statistical significance for the publication year, research area and research methods. Egger's test showed publication bias in the IBD studies (  =  .006  <  .05) but no publication bias for the CD (  =  .275>.05) and UC studies (  =  .537>.05). There was a positive association between antibiotic use in childhood and the subsequently risk of Crohn's disease in non-European countries in the west during 2010-2013. Children in the United States taking antibiotics will have a higher risk of subsequently IBD than Europe, Asia and Australia. CRD42019147648 (PROSPERO).
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http://dx.doi.org/10.1080/00365521.2020.1737882DOI Listing
March 2020

Effect of PA3 on gut microbiota in an in vitro colonic simulation.

Food Sci Nutr 2019 Dec 14;7(12):3883-3891. Epub 2019 Nov 14.

School of Food Science and Bioengineering Zhejiang Gongshang University Hangzhou China.

It has been reported that PA3 has an ability to absorb exogenous purines in the intestine to reduce a risk of gout and hyperuricemia. However, influences of this strain on gut microbiota and their metabolisms remain unclear. Herein, we aimed to investigate the effect of PA3 on microbiota composition and metabolisms. PA3 was isolated from yogurt and supplemented into a single-stage colonic fermentation in a culture volume of 30 ml and subjected to in vitro colonic simulation for 8 days. Microbiota composition was determined with 16S rRNA (V3 + V4) sequencing, and their metabolisms were predicted by PICRUSt. Short-chain fatty acids were measured by GC-MS. We found that PA3 reduced the diversity of microbiota, increased the relative abundances of (73.5%) and (36.5%), and decreased and . Total amount of short-chain fatty acids was found to decline. Fundamental metabolisms, especially nucleotide, was significantly higher after intervention with PA3, but the purine metabolism was lower, which means that PA3 might reduce uric acid concentrations by weakening purine metabolism. Our results indicated that PA3 can survive and play a role in the ascending colon environment. Therefore, the evaluation of the effect of PA3 on intestinal microbes and their metabolisms has great guiding significance for the development of treatment to prevent gout.
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http://dx.doi.org/10.1002/fsn3.1236DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6924308PMC
December 2019

MiR-361-5p exerts tumor-suppressing functions in gastric carcinoma by targeting syndecan-binding protein.

Anticancer Drugs 2020 02;31(2):131-140

Department of Pathology, Women's Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing, China.

MiR-361-5p, a tumor-related microRNA, has been reported to be implicated in the tumorigenesis and progression of diverse types of human malignancies; however, its role in gastric carcinoma remains unclear. This study aimed to explore the biological role of miR-361-5p in gastric carcinoma and clarify the potential mechanisms involved. In the present study, miR-361-5p was found to be significantly downregulated in both gastric carcinoma tissues and cell lines. Functional studies demonstrated that enhanced expression of miR-361-5p suppressed gastric carcinoma cell proliferation in vitro, inhibited tumor growth in vivo, and induced gastric carcinoma cell apoptosis. Moreover, the tumor-suppressing effects of miR-361-5p in gastric carcinoma were abrogated by the miR-361-5p inhibitor treatment. Notably, syndecan-binding protein was downregulated by miR-361-5p via direct binding to its 3' untranslated region in gastric carcinoma cells. Furthermore, syndecan-binding protein expression was discovered to be markedly upregulated and inversely correlated with miR-361-5p expression in gastric carcinoma tissues. Mechanistic studies revealed that restoring the expression of syndecan-binding protein alleviated miR-361-5p-induced inhibitory effects on proliferation of gastric carcinoma cells. Taken together, these findings suggest that miR-361-5p functions as a tumor suppressor in gastric carcinoma by directly targeting syndecan-binding protein and that miR-361-5p might be a novel therapeutic target for gastric carcinoma.
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http://dx.doi.org/10.1097/CAD.0000000000000846DOI Listing
February 2020

The intestinal microbiota of lake anchovy varies according to sex, body size, and local habitat in Taihu Lake, China.

Microbiologyopen 2020 01 29;9(1):e00955. Epub 2019 Nov 29.

Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Changjiang River, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, CAFS, WuXi, China.

Lake anchovy (Coilia ectenes taihuensis) is a sedentary, dominant fish species that forms an unmanaged fishery in Taihu Lake, eastern China. The environment and developmental stage of lake anchovy are likely important drivers of their gut microbiome, which is linked to host health and development. To investigate the relationship between the gut microbiome and three defined factors (fish sex, fish body size, and the local habitat), high-throughput sequencing of the 16S ribosomal RNA gene was used to study the microorganisms of 184 fish samples and four water samples collected in Taihu Lake. Four dominant bacterial phyla (Proteobacteria, Firmicutes, Planctomycetes, and Cyanobacteria) were present in all fish samples. We compared the microbial communities of males and females and found that the relative abundance of Corynebacteriaceae was significantly higher in males than in females, while the opposite trend was detected for Sphingomonadaceae. We also discovered that the relative abundance of Firmicutes was positively correlated with fish body size and that the proportions of Proteobacteria and Tenericutes were lower in larger fish than in fish of other sizes. Finally, we found that the difference in microbial richness between eastern and northern Taihu Lake was the most marked. Lake anchovy was rich in Lactobacillus and Clostridium in the eastern site, while those in the northern site had the highest abundance of Sphingomonas and Methylobacterium, suggesting that the local habitat may also influence the intestinal microbiome. These findings will not only help researchers understand the community composition of the intestinal microflora of lake anchovy but also contribute to the protection of fish resources in Lake Taihu and the sustainable use of lake anchovy.
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http://dx.doi.org/10.1002/mbo3.955DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6957416PMC
January 2020

Norcantharidin Enhances High Concentrations of Fetal Bovine Serum-Induced Apoptosis in Human Mesangial Cells by Regulating the Mitogen-Activated Protein Kinase Signaling Pathway.

Kidney Blood Press Res 2019 29;44(6):1339-1351. Epub 2019 Oct 29.

Department of Nephrology, The People's Hospital of Guangxi Zhuang Autonomous Region, Qingxiu, China.

Aim: This study aimed to investigate the effect of norcantharidin (NCTD) on human mesangial cells (HMCs) apoptosis in vitro and further examine its molecular mechanism.

Methods: HMCs were divided into 5 groups: control group, 25% fetal bovine serum (FBS)-treated group, and NCTD groups (NCTD [2.5, 5 and 10 µg/mL] + 25% FBS, respectively). Cell proliferation was determined by MTT assay, while apoptosis was evaluated by Hoechest 33258 staining, the level of cytochrome c, immunohistochemistry, and apoptotic-related proteins/gene expression.

Results: Cell viability was inhibited in NCTD-treated HMCs in a dose-dependent manner. The number of apoptotic cells and the content of cytochrome c were significantly increased by NCTD treatment but that of mitochondrial membrane was decreased. Moreover, the expression of bcl-2 and caspase-3 was prompted by NCTD, but the expression of bax, MMP-2, and MMP-9 in 25% FBS-treated HMCs was inhibited. In addition, NCTD markedly unregulated the expression of apoptosis-related gene/protein, including p-Erk1/2, phosphorylated-Jun N-terminal kinase (JNK), p-p38, and p53.

Conclusion: NCTD enhances 25% FBS-treated HMC apoptosis in vitro, and this effect may be attributed to the modulation of the ERK, JNK, and p38 mitogen-activated protein kinase signaling pathways.
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http://dx.doi.org/10.1159/000502524DOI Listing
June 2020
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