Publications by authors named "Kun Yan"

234 Publications

Inhibition of Hepatitis B Virus by AAV8-Derived CRISPR/SaCas9 Expressed From Liver-Specific Promoters.

Front Microbiol 2021 26;12:665184. Epub 2021 Jun 26.

State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, China.

Curative therapies for chronic hepatitis B virus (HBV) infection remain a distant goal, and the persistence of stable covalently closed circular DNA (cccDNA) during HBV replication is a key barrier that is hard to break through using the drugs currently approved for HBV treatment. Due to the accuracy, efficiency, and cost-effectiveness of genome editing, CRISPR/Cas technologies are being widely used for gene therapy and in antiviral strategies. Although CRISPR/Cas could possibly clear cccDNA, ensuring its safety is requirement for application. In our study, we analyzed the liver specificity of several promoters and constructed candidate promoters in the CRISPR/ Cas9 (SaCas9) system combined with hepatotropic AAV8 (whereby AAV refers to adeno-associated virus) to verify the efficacy against HBV. The results revealed that the reconstructed CRISPR/SaCas9 system in which the original promoter replaced with a liver-specific promoter could still inhibit HBV replication both and . Three functional guide RNAs (gRNAs), T, T, and T, which target the conserved regions of different HBV genotypes, demonstrated consistently better anti-HBV effects with different liver-specific promoters. Moreover, the three gRNAs inhibited the replication of HBV genotypes A, B, and C to varying degrees. Under the action of the EnhII-Pa1AT promoter and AAV8, the expression of SaCas9 was further decreased in other organs or tissues in comparison to liver. These results are helpful for clinical applications in liver by ensuring the effects of the CRISPR/Cas9 system remain restricted to liver and, thereby, reducing the probability of undesired and harmful effects through nonspecific targeting in other organs.
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http://dx.doi.org/10.3389/fmicb.2021.665184DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8271097PMC
June 2021

HIFU for the treatment of gastric cancer with liver metastases with unsuitable indications for hepatectomy and radiofrequency ablation: a prospective and propensity score-matched study.

BMC Surg 2021 Jul 12;21(1):308. Epub 2021 Jul 12.

Department of General Surgery, HwaMei Hospital, University of Chinese Academy of Sciences, Northwest Street 41, Haishu District, Ningbo, 315010, Zhejiang, China.

Background: The purpose of this study was to explore the efficacy and safety of high intensity focused ultrasound (HIFU) in gastric cancer with liver metastasis (GCLM) patients who were contraindicated for either hepatectomy or radiofrequency ablation (RFA).

Methods: This is a prospective, observational study on GCLM patients with 1-3 liver metastases. The primary gastric lesions were thoroughly resected and any case that exhibited extra-hepatic metastasis was excluded. A 1:2:2 propensity score-matching analysis was performed using a logistic regression model on the HIFU group, best supportive care (BSC) group, and palliative chemotherapy (PC) group. The primary endpoints include progression-free survival (PFS) and overall survival (OS).

Results: Forty patients were finally included, there were 8 cases in HIFU group, 16 cases in BSC group, and 16 cases in PC group. The median follow-up time for the entire cohort was 10 months. The median PFS was 16.5 months in HIFU group, 2 months in BSC group, and 5 months in PC group. The median OS was 27.5 months in the HIFU group, 7 months in the BSC group, and 11.5 months in the PC group. Additionally, no grade 3 or higher adverse events occurred in the HIFU group.

Conclusion: The results of this study showed that HIFU treatment could improve the long-term prognosis of GCLM patients without a significant increase in the occurrence of adverse events. Compared with PC and BSC, HIFU is the preferred treatment option when GCLM patients without extra-hepatic metastasis are unable to undergo either surgery or RFA.
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http://dx.doi.org/10.1186/s12893-021-01307-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8273961PMC
July 2021

Prognostic Value of Pretreatment Overweight/Obesity and Adipose Tissue Distribution in Resectable Gastric Cancer: A Retrospective Cohort Study.

Front Oncol 2021 24;11:680190. Epub 2021 Jun 24.

Department of Thoracic Surgery, HwaMei Hospital, University of Chinese Academy of Sciences, Ningbo, China.

Background: This is a study aimed at exploring the relationship between pretreatment overweight/obesity, adipose tissue distribution, and long-term prognosis of gastric cancer.

Methods: A total of 607 gastric cancer patients were involved in the retrospective cohort study. Overweight/obese patients were defined as body mass index (BMI) greater than 25 kg/m, and adipose tissue distribution parameters, including visceral adipose tissue (VAT), subcutaneous adipose tissue (SAT), and VAT/SAT ratio were measured at the level of the third lumbar vertebra using computerized tomography images within 15 days before the surgery. Multiple Cox regression models were applied to evaluate the association between overweight/obesity and disease-specific survival (DSS) of gastric cancer, and covariates including age, gender, T stage, N stage, and chemotherapy were adjusted. Furthermore, multiple Cox regression models were performed to evaluate the association between adipose tissue distribution parameters and DSS of gastric cancer; except for covariates mentioned above, overweight/obesity was adjusted additionally.

Results: Overweight/obesity was a predictive factor (HR = 0.61, 95% CI: 0.37-0.99) for the prognosis of gastric cancer. After additionally adjusting for overweight/obesity, high SAT percentage was an independent protective factor (HR = 0.59, 95% CI: 0.36-0.96), while high VAT percentage (HR = 1.68, 95% CI: 1.06-2.68) and high VAT/SAT ratio (HR = 1.99, 95% CI: 1.19-3.34) were independent risk factors for DSS of gastric cancer. Compared with other patients (overweight/obesity with low VAT/SAT ratio group, non-overweight/obesity or high VAT/SAT ratio group), patients in the non-overweight/obesity with high VAT/SAT ratio group had a worse prognosis (HR = 1.89, 95% CI: 1.28-2.77).

Conclusion: These results suggest that overweight/obesity is a predictive factor for the prognosis of gastric cancer. The VAT/SAT ratio could be used as a promising prognostic factor for gastric cancer. Therefore, in preoperative evaluation of gastric cancer patients, attention should be paid not only to BMI but also to adipose tissue distribution.
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http://dx.doi.org/10.3389/fonc.2021.680190DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8264507PMC
June 2021

Interpenetrating polysaccharide-based hydrogel: A dynamically responsive versatile medium for precisely controlled synthesis of nanometals.

Mater Sci Eng C Mater Biol Appl 2021 Aug 27;127:112211. Epub 2021 May 27.

Hubei Key Laboratory of Advanced Textile Materials & Application, Hubei International Scientific and Technological Cooperation Base of Intelligent Textile Materials &Application, Key Laboratory of Textile Fiber & Product, Ministry of Education, Wuhan Textile University, Wuhan 430200, China. Electronic address:

Herein, we reported an interpenetrating polysaccharide-based hydrogel in which carboxymethyl chitosan (CMC) chains were physically dispersed throughout the thermoplastic elastomer gel network has been developed as a versatile platform for precisely controlled synthesis of nanometals. Results indicated the interpenetrated CMC chains could serve as multifunctional fillers for metal ions adsorption and stabilization while the thermally reconfigurable agarose (Agar) gel medium provides three-dimensional semi-solid framework for entrapping and recollecting of the fabricated nanometals. Specifically, the CMC chains were found to strongly coordinate with silver ions as a dynamically responsive metal-biopolymer complex within the bulk gel network as confirmed by the enhanced mechanical properties and regulated shape memory performances. Moreover, by varying CMC concentrations and coupling with a layer-stacking approach, multiple biochemical gradients could be facilely generated for in-situ synthesis of silver nanoparticles, achieving a narrow size of ~7 nm, confined sphere-shape and high concentrations. The monodispersed nanometals are confirmed to be highly active (e.g., considerable catalytic performance), and which could be easily recycled from the bulk gel system via a heating treatment. Thus, this work would provide a generic methodology for the multifunctional metallogel assembly and great possibility for controllable and largescale synthesis of noble nanometals toward biomedical applications.
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http://dx.doi.org/10.1016/j.msec.2021.112211DOI Listing
August 2021

Cu-PSMA-BCH: a new radiotracer for delayed PET imaging of prostate cancer.

Eur J Nucl Med Mol Imaging 2021 Jun 25. Epub 2021 Jun 25.

Key Laboratory of Carcinogenesis and Translational Research, Ministry of Education/Beijing), NMPA Key Laboratory for Research and Evaluation of Radiopharmaceuticals (National Medical Products Administration), Department of Nuclear Medicine, Peking University Cancer Hospital & Institute, No. 52 Fu-Cheng Rd, Beijing, 100142, China.

Purpose: Develop a Cu labeled radiopharmaceutical targeting prostate specific membrane antigen (PSMA) and investigate its application for prostate cancer imaging.

Methods: Cu-PSMA-BCH was prepared and investigated for stability, PSMA specificity, and micro-PET imaging. With the approval of Ethics Committee of Beijing Cancer Hospital (No. 2017KT97), PET/CT imaging in 4 patients with suspected prostate cancer was performed and the radiation dosimetry was estimated. Then, PSMA PET-ultrasound image-guided biopsies were performed on 3 patients and the fine needle aspirates were further performed for autoradiography and immunohistochemistry analysis.

Results: Cu-PSMA-BCH was prepared with high radiochemical yield and stability. In vivo study showed higher uptake in PSMA ( +) 22Rv1 cells than PSMA ( -) PC-3 cells (5.59 ± 0.36 and 1.97 ± 0.22 IA%/10 cells at 1 h). It accumulated in 22Rv1 tumor with increasing radioactivity uptake and T/N ratios from 1 to 24 h post-injection. In patients with suspected prostate cancer, SUVmax and T/N ratios increased within 24 h post-injection. Compared with image at 1 h post-injection, more tumor lesions were detected at 6 h and 24 h post-injection. The human organ radiation dosimetry showed gallbladder wall was most critical, liver and kidneys were followed, and the whole-body effective dose was 0.0292 mSv/MBq. Two fine needle aspirates obtained by PET-ultrasound-guided targeted biopsy showed high radioactive signal by autoradiography, with 100% PSMA expression in cytoplasm and 30% expression in nucleus.

Conclusion: Cu-PSMA-BCH was PSMA specific and showed high stability in vivo with lower uptake in liver than Cu-PSMA-617. Biodistribution in mice and PCa patients showed similar profile compared with other PSMA ligands and it was safe with moderate effective dosimetry. The increased tumor uptake and T/N ratios by delayed imaging may facilitate the detection of small lesions and guiding targeted biopsies.
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http://dx.doi.org/10.1007/s00259-021-05426-9DOI Listing
June 2021

The Incidence, Risk Factors, and Patterns of Peripherally Inserted Central Catheter-Related Venous Thrombosis in Cancer Patients Followed Up by Ultrasound.

Cancer Manag Res 2021 1;13:4329-4340. Epub 2021 Jun 1.

Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), ICU, Peking University Cancer Hospital & Institute, Beijing, People's Republic of China.

Purpose: A peripherally inserted central catheter (PICC) is associated with venous thromboembolism (VTE) especially in patients suffering from cancer. We analyzed the incidence, risk factors, and patterns of PICC-related VTE in cancer patients.

Patients And Methods: Patients with cancer who underwent PICC placement were evaluated retrospectively. Routine, prospective ultrasound post-PICC placement was used for asymptomatic and symptomatic patients to identify VTE. Multivariable logistic regression models with odds ratios (ORs) were used to examine VTE risk factors.

Results: Of 2353 PICCs placed, 165 patients (7.01%) developed PICC-related VTE with a median thrombosis time of 12 days. After adjustment of multivariable analysis, patients with PICC-related VTE were more likely to have a ratio of PICC diameter:vein diameter >0.35 (adjusted OR, 1.689; 95% CI, 1.023-2.789) and high level of triglycerides (1.561; 1.096-2.223). The prevalence of A (adjusted OR, 1.680; 95% CI, 1.009-2.798), B (1.835; 1.137-2.961), and AB (3.275; 1.840-5.829) blood group was significantly higher than that of the O blood group in VTE patients. Venous recanalization was observed in 44.8% (74/165) patients after anticoagulation therapy, and more often in patients with combined deep VTE than in patients with isolated superficial VTE (OR, 17.942; 95% CI, 5.427-59.316). The recanalization time was 20±5 (range, 10-31) days.

Conclusion: The non-O blood group, larger ratio of PICC diameter:vein diameter, and high level of triglycerides were significantly associated with PICC-related VTE. Almost half of cases of PICC-related deep VTE could be reversed by anticoagulation treatment.
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http://dx.doi.org/10.2147/CMAR.S301458DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8179741PMC
June 2021

The 10-year Survival Analysis of Radiofrequency Ablation for Solitary Hepatocellular Carcinoma 5 cm or Smaller: Primary versus Recurrent HCC.

Radiology 2021 Aug 18;300(2):458-469. Epub 2021 May 18.

From the Departments of Ultrasound (X.M.B., W.Y., H.W., S.W., Z.Y.Z., W.W., M.H.C., K.Y.) and Oncology Surgery (M.C.), Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Peking University Cancer Hospital and Institute, No. 52 Fucheng Rd, District of Haidian, Beijing 100142, China; and Division of Image-Guided Therapy, Department of Radiology, Hadassah Hebrew University Medical Center, Jerusalem, Israel (S.N.G.).

Background Although favorable outcomes have been reported with radiofrequency ablation (RFA) for limited hepatocellular carcinoma (HCC), the efficacy of this treatment for recurrent HCC has not been thoroughly investigated. Purpose To compare the long-term outcomes and analyze the prognostic factors for outcomes after RFA for initial HCC versus as a second-line treatment for recurrent HCC. Materials and Methods This retrospective study included 560 patients with solitary tumors 5 cm or smaller (263 initial HCCs, 297 -recurrent HCCs) who underwent percutaneous US-guided RFA from January 2005 to December 2016. Of 297 patients with -recurrent HCC, 134 had previously undergone hepatectomy, 128 had undergone transarterial chemoembolization (TACE), and 35 had undergone local ablation therapy. Overall survival (OS) between initial HCC and recurrent HCC was compared before and after propensity score matching. Prognostic factors for all patients were analyzed with the log-rank test and Cox proportional hazards model. Results A total of 560 patients (mean age, 60 years ± 12 [standard deviation]; 441 men) were evaluated. Before matching, the OS rates at 1, 3, 5, and 10 years were 92.6%, 73.9%, 59.3%, and 39.6%, respectively, in patients with recurrent HCC and 92.8%, 75.4%, 63.3%, and 44.7% in patients with initial HCC ( = .27). After matching, the OS rates at 1, 3, 5, and 10 years were 94.8%, 75.7%, 61.6%, and 47.3% in the initial HCC group and 91.9%, 71.2%, 58.7%, and 45.2% in the recurrent HCC group ( = .32). Among patients with recurrent HCC, no significant difference in mean OS was noted for local recurrence versus distant recurrence (81.6 months ± 5.1 vs 83.8 months ± 6.6, = .82) or previous treatment modality (82.0 months ± 7.3 in the resection group, 82.7 months ± 5.3 in the TACE group, and 79.3 months ± 10.8 in the local ablation group; = .83). Local tumor progression after previous local ablation (10 of 35 [28.6%]) was higher than that after previous hepatectomy (15 of 134 [11.2%], = .04). Multivariable analysis demonstrated that tumor size (hazard ratio, 1.58; 95% CI: 1.06, 2.36; = .02), portal hypertension (hazard ratio, 1.52; 95% CI: 1.03, 2.26; = .04), Child-Pugh class (hazard ratio, 2.01; 95% CI: 1.02, 3.96; = .045), and serum α-fetoprotein level (hazard ratio, 1.62; 95% CI: 1.10, 2.39; = .01) were independent predictive factors for recurrent HCC outcomes. Conclusion Radiofrequency ablation provides similar long-term survival for solitary hepatocellular carcinoma of 5 cm or less, regardless of whether treatment is initial or salvage therapy. © RSNA, 2021
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http://dx.doi.org/10.1148/radiol.2021200153DOI Listing
August 2021

Nomogram including chemotherapy response for prediction of intrahepatic progression-free survival in patients with colorectal liver metastasis through chemotherapy followed by radiofrequency ablation.

Int J Hyperthermia 2021 ;38(1):633-639

Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Department of Ultrasound, Peking University Cancer Hospital & Institute, Beijing, China.

Background: Radiofrequency ablation (RFA) is being considered as the favorable treatment option for unresectable colorectal cancer liver metastases (CRLM) receiving chemotherapy, yet there still exist challenges for recurrence after RFA. The present study aims to establish an effective nomogram to predict intrahepatic progression-free survival (PFS) and select RFA candidates.

Methods: Patients with unresectable CRLM treated with chemotherapy followed by RFA between 2010 and 2016 were enrolled in this study. The nomogram to predict intrahepatic PFS was established based on multivariable Cox regression analysis. The predictive performance of the nomogram was assessed according to the C-index, calibration plots and Kaplan-Meier curve.

Results: Of a total of 158 patients, the earlier new intrahepatic metastases over local tumor progression were observed in 157 patients during the follow-up, and the mean intrahepatic PFS was 16.9 ± 1.4 months in the present cohort. The optimal cutoff value of tumor size after chemotherapy was identified as 16 mm by X-tile analysis. Based on multivariate analysis, independent prognostic factors for intrahepatic PFS included primary positive lymph nodes, multiple metastases, tumor size >16 mm, no primary lesion resection, mutant KRAS and PD response after chemotherapy. The nomogram was established to predict intrahepatic PFS based on all independent factors, which achieved favorable discrimination and calibration.

Conclusion: This study firstly established the nomogram to predict intrahepatic PFS for unresectable CRLM patients receiving chemotherapy followed by RFA. It can facilitate the selection of RFA candidates, and help both surgeons and patients choose individualized regimens in the treatment decision.
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http://dx.doi.org/10.1080/02656736.2021.1912415DOI Listing
July 2021

The Functional and Antiviral Activity of Interferon Alpha-Inducible IFI6 Against Hepatitis B Virus Replication and Gene Expression.

Front Immunol 2021 1;12:634937. Epub 2021 Apr 1.

State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, China.

Hepatitis B virus is an enveloped DNA virus, that infects more than three hundred and sixty million people worldwide and leads to severe chronic liver diseases. Interferon-alpha inducible protein 6 (IFI6) is an IFN-stimulated gene (ISG) whose expression is highly regulated by the stimulation of type I IFN-alpha that restricts various kinds of virus infections by targeting different stages of the viral life cycle. This study aims to investigate the antiviral activity of IFI6 against HBV replication and gene expression. The IFI6 was highly induced by the stimulation of IFN-α in hepatoma cells. The overexpression of IFI6 inhibited while knockdown of IFI6 elevated replication and gene expression of HBV in HepG2 cells. Further study determined that IFI6 inhibited HBV replication by reducing EnhII/Cp of the HBV without affecting liver enriched transcription factors that have significant importance in regulating HBV enhancer activity. Furthermore, deletion mutation of EnhII/Cp and CHIP analysis revealed 100 bps (1715-1815 nt) putative sites involved in IFI6 mediated inhibition of HBV. Detailed analysis with EMSA demonstrated that 1715-1770 nt of EnhII/Cp was specifically involved in binding with IFI6 and restricted EnhII/Cp promoter activity. Moreover, IFI6 was localized mainly inside the nucleus to involve in the anti-HBV activity of IFI6. analysis based on the hydrodynamic injection of IFI6 expression plasmid along with HBV revealed significant inhibition of HBV DNA replication and gene expression. Overall, our results suggested a novel mechanism of IFI6 mediated HBV regulation that could develop potential therapeutics for efficient HBV infection treatment.
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http://dx.doi.org/10.3389/fimmu.2021.634937DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8047077PMC
April 2021

Antiviral Activity of Interferon Alpha-Inducible Protein 27 Against Hepatitis B Virus Gene Expression and Replication.

Front Microbiol 2021 31;12:656353. Epub 2021 Mar 31.

State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, China.

Despite the availability of effective vaccines, hepatitis B virus (HBV) is still a major health issue, and approximately 350 million people have been chronically infected with HBV throughout the world. Interferons (IFNs) are the key molecules in the innate immune response that restrict several kinds of viral infections the induction of hundreds of IFN-stimulated genes (ISGs). The objective of this study was to confirm if interferon alpha-inducible protein 27 (IFI27) as an ISG could inhibit HBV gene expression and DNA replication both in cell culture and in a mouse model. In human hepatoma cells, IFI27 was highly induced by the stimulation of IFN-alpha (IFN-α), and it potentiated the anti-HBV activity. The overexpression of IFI27 inhibited, while its silencing enhanced the HBV replication in HepG2 cell. However, the knocking out of IFI27 in HepG2 cells robustly increases the formation of viral DNA, RNA, and proteins. Detailed mechanistic analysis of the HBV genome showed that a sequence [nucleotide (nt) 1715-1815] of the EnhII/Cp promoter was solely responsible for viral inhibition. Similarly, the hydrodynamic injection of IFI27 expression constructs along with the HBV genome into mice resulted in a significant reduction in viral gene expression and DNA replication. In summary, our studies suggested that IFI27 contributed a vital role in HBV gene expression and replication and IFI27 may be a potential antiviral agent for the treatment of HBV.
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http://dx.doi.org/10.3389/fmicb.2021.656353DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8044325PMC
March 2021

High-intensity focused ultrasound alone or combined with transcatheter arterial chemoembolization for the treatment of hepatocellular carcinoma with unsuitable indications for hepatectomy and radiofrequency ablation: a phase II clinical trial.

Surg Endosc 2021 Mar 31. Epub 2021 Mar 31.

Department of Tumor HIFU Therapy, HwaMei Hospital,, University of Chinese Academy of Sciences, Northwest Street 41, Haishu District, Ningbo, 315010, Zhejiang, China.

Objectives: This study aims to evaluate the efficacy and safety of high-intensity focused ultrasound (HIFU) alone or combined with transcatheter arterial chemoembolization (TACE) for patients with hepatocellular carcinoma (HCC) but were contraindicated for hepatectomy and radiofrequency ablation (RFA).

Methods: Patients between 20 and 80 years of age with 1-3 foci of HCC were selected. Included patients have had primary or recurrent liver lesions with no evidence of extra-hepatic metastasis prior to the study. Patients were treated with ultrasound-guided HIFU alone or HIFU combined with TACE (treated with TACE once within 4 weeks prior to receiving HIFU).

Results: Thirty-seven patients were enrolled, for a total of 45 lesions. The 2-year local control (LC) rate was 73.0% and the median LC time was 22 months. The 2-year progression-free survival (PFS) was 29.7% and the median PFS time was 9 months. Finally, the 2-year overall survival (OS) was 70.3%, and the median OS time was 24 months. The most common adverse events (AEs) were elevated liver enzymes, followed by fatigue, and pain, no grade 4 AEs or death occurred. Multivariate analysis showed that age, Child-Pugh class, and the number of tumors were independent prognostic factors for PFS and that the AFP levels and the number of tumors were significantly correlated with the OS.

Conclusions: This study indicates that the HIFU/HIFU combined with TACE treatment is safe, and is capable of achieving both a good LC rate and a considerably good prognosis. The procedure should be considered for patients who were deemed unsuitable for other local treatments.
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http://dx.doi.org/10.1007/s00464-021-08465-3DOI Listing
March 2021

Electrochemically deposition of catechol-chitosan hydrogel coating on coronary stent with robust copper ions immobilization capability and improved interfacial biological activity.

Int J Biol Macromol 2021 Jun 26;181:435-443. Epub 2021 Mar 26.

Cheeloo College of Medicine, Shandong University, 250012, China; Department of Cardiology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230036, China. Electronic address:

Establishing a facile and versatile strategy to confer coronary stent with improved interfacial biological activity is crucial for novel cardiovascular implants. Developing a coating with NO release ability catalyzed by metal ions, such as copper, will be highly advantageous for the functionalized surface modification of metal stents. However, most available strategies involve drawbacks of low efficiency, complex processes, and toxic chemicals. Therefore, in this study, we report a green and facile electrobiofabrication method to construct the bioactive hydrogel coating by combining chitosan, catechol groups and copper ions on coronary stent and titanium surfaces. Experimental results demonstrated that the chitosan hydrogel coating can be precisely controlled synthesis via electrochemical deposition and serves as a versatile platform for copper ions immobilization. Additionally, mussel-inspired catechol groups could be chemically grafted on chitosan chains to further enhance the film mechanical properties and binding abilities of copper ions. Moreover, in vitro cell biocompatibility and catalyzed NO-generation activity have also been accessed and which suggesting great possibilities for biomedical applications. Therefore, by coupling the electrobiofabrication approach and multi-functionalities of the hybrid film, this report would advance the development of biomimetic hydrogel coating for vascular engineering (e.g., coronary stent) and other biomedical devices.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.03.158DOI Listing
June 2021

Electrochemical synthesis of chitosan/silver nanoparticles multilayer hydrogel coating with pH-dependent controlled release capability and antibacterial property.

Colloids Surf B Biointerfaces 2021 Jun 19;202:111711. Epub 2021 Mar 19.

School of Resource and Environmental Science, Hubei International Scientific and Technological Cooperation Base of Sustainable Resource and Energy, Hubei Engineering Center of Natural Polymers-Based Medical Materials, Hubei Biomass-Resource Chemistry and Environmental Biotechnology Key Laboratory, Wuhan University, Wuhan, 430079, China. Electronic address:

By coupling in situ electrochemical synthesis of silver nanoparticles (AgNPs) with the pre-deposited chitosan multilayer hydrogel, a novel type of nanocomposite coating was successfully fabricated on the stainless-steel needle electrode. Experimental results demonstrated the chitosan film can serve as a versatile medium for metal salt adsorption and stabilization, and finally electrochemical reduction of loaded silver ions to nanoparticles. The AgNPs were fabricated with a spherical shape and an average size of ∼15 nm endowing considerable antibacterial property to the hydrogel. Furthermore, the unique layered architecture consisted of porous segments and compact boundaries is almost retained, resulting in a pH-dependent and staged release pattern of silver nanoparticles based on acid triggered dissolution of the multi-membrane layer by layer. Thus, considering the mild synthesizing approach, multi-functionalities and relatively low cytotoxicity, this antibacterial hydrogel would show great potential either to be used as a newly coating material for interfacial improvement of implants or as a free-standing film after being peeled off for wound dressing.
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http://dx.doi.org/10.1016/j.colsurfb.2021.111711DOI Listing
June 2021

Coronavirus Disease 2019 (COVID-19): Prevention and Control in Gynecological Outpatient Clinic.

Front Public Health 2020;8:618494. Epub 2021 Jan 8.

Department of Gynecology, Maternal and Child Health Hospital of Hubei Province, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

The pandemic of coronavirus disease 2019 (COVID-19) has become a major public health challenge around the world, and outbreaks of the SARS-CoV-2 have constituted a public health emergency of international concern. Infection control measures are necessary to prevent further spread of the virus and to help control the epidemic situation. Due to the characteristics of gynecological settings, the risk of cross infection between patients and gynecologic practitioners can be high, strict and effective infection control protocols are urgently needed. This article, based on our experience and relevant guidelines and research, introduces prevention and control measures for use in gynecological outpatient clinics and provides recommended management for gynecologists in (potentially) affected areas.
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http://dx.doi.org/10.3389/fpubh.2020.618494DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7820776PMC
February 2021

Handedness Inversion of Chiral 3-Aminophenol Formaldehyde Resin Nanotubes Mediated by Metal Coordination.

Angew Chem Int Ed Engl 2021 Mar 25;60(14):7759-7769. Epub 2021 Feb 25.

Hubei Key Laboratory of Advanced Textile Materials & Application, Wuhan Textile University, Yangguang Road 1, Wuhan, 430200, P. R. China.

Precise adjustment of microstructure and handedness of chiral nanomaterials is important to regulate their properties and performance. Herein, helical 3-aminophenol formaldehyde resin (APF) nanotubes and corresponding carbonaceous nanotubes with controllable handedness and optical activity were obtained via an external metal ion-mediated supramolecular co-templating method in an enantiomerically pure template system, in which an appropriate amount of Mn (Co or Ni ) with moderate coordination abilities can reverse the spatial arrangement of the phenylglycine-based amphiphilic template molecules through metal coordination. Different stacking modes of coordination complexes in disparate metal ion systems lead to diverse helical senses (diameter and pitch) of the obtained helical APF. In addition, this coordination mode of metal intervention can be applied to other amine-based helical polymer synthesis systems, which paves the way for the design of high-quality chiral nanomaterials with satisfactory physical parameters and properties.
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http://dx.doi.org/10.1002/anie.202013790DOI Listing
March 2021

Relationship of the lunar cycle with Down's syndrome screening and its effects.

Medicine (Baltimore) 2020 Dec;99(52):e23792

Gansu Center for Clinical Laboratory, Gansu Provincial Hospital, Lanzhou.

Abstract: The present study aimed to analyze the positive rate of Down syndrome in second-trimester pregnant women in 1 lunar cycle and calculate variation coefficients of daily person numbers and daily positive rates in this population so as to explore the relationship of the lunar cycle with Down syndrome screening and its effects.Data and laboratory results of 51,450 second-trimester pregnant women who underwent Down syndrome screening between May 2013 and June 2017 of the Chinese lunar calendar were collected. The patients were allocated into groups according to the time period of the lunar cycle based on the start date of their last menstruation. In the Chinese lunar calendar, 1 lunar cycle is divided into eight time periods. The positive rate of Down syndrome in pregnant women with the same start date of last menstruation and changes in their variation coefficients of daily person numbers and daily positive rates were analyzed.The findings displayed the lowest positive rate of Down syndrome in the group of pregnant women who had the start date of last menstruation within the full-moon time period. The greatest variation coefficients of daily person numbers and daily positive rates were also found in the same group.The study showed that the moon indeed affected pregnant women, and the effect reached the peak by the full moon. The effect interfered with the body homeostasis of pregnant women to a certain degree. Therefore, the relationship of the lunar cycle with Down syndrome screening reflected the interaction of the moon with the homeostasis of pregnant women.
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http://dx.doi.org/10.1097/MD.0000000000023792DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7769297PMC
December 2020

Specific Inhibitor of Matrix Metalloproteinase Decreases Tumor Invasiveness After Radiofrequency Ablation in Liver Tumor Animal Model.

Front Oncol 2020 18;10:561805. Epub 2020 Nov 18.

Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Department of Ultrasound, Peking University Cancer Hospital & Institute, Beijing, China.

Objective: To determine whether the specific inhibitor of matrix metalloproteinase (MMP)-batimastat (BB-94)-could decrease the progression of liver tumor after radiofrequency ablation (RFA) and achieve better therapeutic efficacy in an animal model.

Methods: experiments, the proliferation of H22 liver tumor cells was detected by CCK 8 assay and cell migration was detected by Transwell method. experiments, H22 murine liver tumors were used. First, 32 mice with one tumor were randomized into four groups (n = 8 each group): control (PBS only), RFA alone (65°C, 5 min), BB-94 (30 mg/kg), RFA+BB-94. The growth rate of the residual tumor and the end point survival were calculated and the pathologic changes were evaluated. Secondly, a total of 48 tumors in 24 animals (paired tumors) were randomized into three groups (n = 8 each group): control, RFA alone, RFA+BB-94. Each mouse was implanted with two tumors subcutaneously, one tumor was treated by RFA and the other was evaluated for distant metastasis after applying BB-94.

Results: , the proliferation assay demonstrated higher proliferation ability after heat treatment (0.82 ± 0.07 1.27 ± 0.08, P = 0.008), and it could be inhibited by BB-94 (1.27 ± 0.08 0.67 ± 0.06, P = 0.001). In the cell migration assay, the H22 cells demonstrated enhanced tumor invasiveness in the heat group than the control group (33.7 ± 2.1 19.7 ± 4.9, P = 0.011). And it could be significantly suppressed after BB-94 incubation (33.7 ± 2.1 23.0 ± 4.6, P = 0.009). With one tumor animal, the growth rate of the residual tumor in the BB-94+RFA group was slower than that in the RFA alone group (P = 0.003). And combination of BB-94 could significantly prolong the survival of the mice (40.3 ± 1.4d 47.1 ± 1.3d, P = 0.002). The expression of CD31 and VEGF at the coagulation margin were decreased after combined with BB-94. With two tumors animal, the growth of metastasis tumor in the BB-94+RFA group was slower than that in the RFA group (P < 0.001).

Conclusion: BB-94 combined with RFA reduced the invasiveness of the liver tumor and improved the end-point survival. Our data suggested that targeting the MMP process with the specific inhibition could help to increase overall ablation efficacy.
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http://dx.doi.org/10.3389/fonc.2020.561805DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7709861PMC
November 2020

Can Radiofrequency Ablation Replace Liver Resection for Solitary Colorectal Liver Metastasis? A Systemic Review and Meta-Analysis.

Front Oncol 2020 17;10:561669. Epub 2020 Nov 17.

Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Department of Ultrasound, Peking University Cancer Hospital and Institute, Beijing, China.

Radiofrequency ablation (RFA) can be a favorable option for patients with colorectal liver metastasis (CRLM). However, current reports about the therapeutic efficacy of liver resection (LR) and RFA for colorectal liver metastasis (CRLM) still remain controversial, especially for solitary CRLM. Therefore, this meta-analysis was performed to evaluate the therapeutic efficacy between LR and RFA for solitary CRLM. First, a comprehensive search for published studies was conducted using PubMed, the Cochrane Library Central, and Web of Science. Each study was reviewed and data extracted. In this meta-analysis, 10 studies (11 study arms) were finally included. The meta-analysis was performed using risk ratio (RR) and random effect model or fixed effect model, in which 95% confidence intervals (95% CI) for RR were calculated. The primary outcomes were disease-free survival (DFS) and overall survival (OS) at 1, 3, or 5 years plus complication rate. The results showed that patients treated by LR achieved better PFS and OS than those by RFA, but subgroup analysis and meta-regression displayed that the efficacy of RFA was equivalent to that of LR in solitary CRLM, when conditions were limited to tumors of ≤ 3 cm and fewer synchronous metastasis in the publication years 2011-2018. Meanwhile, RFA achieved lower complication rates when compared with LR. In conclusion, although patients treated by RFA cannot achieve better PFS and OS than those by LR, RFA can be considered a viable treatment option for solitary CRLM, with potentially lower complication rates.
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http://dx.doi.org/10.3389/fonc.2020.561669DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7706822PMC
November 2020

Contrast-Enhanced Intraoperative Ultrasound Improved Sensitivity and Positive Predictive Value in Colorectal Liver Metastasis: a Systematic Review and Meta-Analysis.

Ann Surg Oncol 2021 Jul 27;28(7):3763-3773. Epub 2020 Nov 27.

Key Laboratory of Carcinogenesis and Translational Research, Hepatopancreatobiliary Surgery Department I, School of Oncology, Beijing Cancer Hospital and Institute, Peking University, Ministry of Education, Beijing, People's Republic of China.

Background: The diagnostic accuracy of imaging modalities in colorectal cancer liver metastases (CRLM) has improved in recent years, therefore the role of current imaging techniques needs to be defined.

Objective: The aim of this study was to assess the diagnostic performance of magnetic resonance imaging, preoperative imaging (magnetic resonance imaging or computed tomography), intraoperative ultrasound, and contrast-enhanced intraoperative ultrasound in the detection of CRLM.

Materials And Methods: Eligible trials published before 30 March 2020 were identified from the EMBASE, PubMed, Web of Science, and Cochrane Library databases, and descriptive and quantitative data were extracted. Study quality was evaluated for the identified studies and a random-effects model was used to determine the integrated diagnosis estimation. Meta-regression was implemented to explore the possible contributors to heterogeneity.

Results: Overall, 13 studies were included for analysis, comprising 682 patients with a total of 2303 liver lesions. The pooled sensitivity, specificity, and diagnostic odds ratio of contrast-enhanced intraoperative ultrasound were 0.94 (95% confidence interval [CI] 0.89-0.97), 0.83 (95% CI 0.67-0.92), and 79 (95% CI 32-196), respectively. The overall weighted area under the curve was 0.96 (95% CI 0.94-0.97). In univariate meta-regression analysis, disappearing liver metastasis, contrast agent, and Kupffer phase were the potent sources of heterogeneity; however, in multivariate meta-regression, no definite variable was the source of the study heterogeneity.

Conclusion: Contrast-enhanced intraoperative ultrasound demonstrated a high sensitivity and specificity for screening CRLM.
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http://dx.doi.org/10.1245/s10434-020-09365-xDOI Listing
July 2021

Inside-outside Ag nanoparticles-loaded polylactic acid electrospun fiber for long-term antibacterial and bone regeneration.

Int J Biol Macromol 2021 Jan 21;167:1338-1348. Epub 2020 Nov 21.

College of Chemical Engineering, Xinjiang Normal University, Urumqi 830054, Xinjiang, PR China. Electronic address:

Bone infections caused by bacteria during bone graft implantations can impair the ability of bone tissue repair, which is currently a clinical problem. In this study, the electrospinning technique was used to prepare a polylactic acid (PLLA)/silver (Ag) composite fiber, in which the silver nanoparticles (Ag-NPs) were uniformly distributed on the inner surface of PLLA fibers; dopamine (DA) was self-polymerized on the composite fiber surface to construct the adhesive polydopamine (PDA) film and chitosan (CS) was used to regulate Ag in situ through pulse electrochemical deposition for the construction of a stable Ag-NPs coating (CS/Ag), achieving the steady and slow release of Ag-NPs, therefore accomplishing the construction of a "inside-outside" Ag-NPs-loaded PLLA/[email protected]@CS/Ag composite fiber with dual functions of long-lasting antibacterial effect as well as bone regeneration promotion ability. The study results showed that the composite fiber has an excellent antibacterial effect against E. coli and S. aureus, and good osteoinductive and angiogenic properties. In summary, under the dual regulations of the strong adhesion of PDA and CS chelation, the "inside-outside" Ag-NPs-loaded composite fiber was endowed with good physiological stability, long-term antibacterial effect and bone infection inhibition ability, making it a promising bone implant material.
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http://dx.doi.org/10.1016/j.ijbiomac.2020.11.088DOI Listing
January 2021

Ten-Year Outcomes of Percutaneous Radiofrequency Ablation for Colorectal Cancer Liver Metastases in Perivascular vs. Non-Perivascular Locations: A Propensity-Score Matched Study.

Front Oncol 2020 16;10:553556. Epub 2020 Oct 16.

Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Department of Ultrasound, Peking University Cancer Hospital & Institute, Beijing, China.

Purpose: To compare long-term outcomes of percutaneous radiofrequency ablation for colorectal liver metastases in perivascular versus non-perivascular locations.

Methods: This retrospective study included 388 consecutive patients with colorectal liver metastases (246 men, 142 women; age range 27-86 years) who underwent percutaneous radiofrequency ablation between January 2006 and December 2018. Propensity-score matching was performed for groups with perivascular and non-perivascular colorectal liver metastases. Rates of accumulative local tumor progression, overall survival, intra/extrahepatic recurrence, and complications were compared between the two groups.

Results: We successfully matched 104 patients each in the perivascular and non-perivascular groups (mean age: 60.1 ± 11.5 and 60.1 ± 11.3 years, respectively). Cumulative local tumor progression rates at 6 months, 1 years, 3 years, and 5 years, respectively, were 8.8%,14.8%, 18.9%, and 18.9% in the perivascular group and 8.8%, 13.1%, 15.5%, and 15.5% in the non-perivascular group. The 1-, 3-, 5-, and 10-year overall survival rates, respectively, were 91.3%, 45.6%, 23.9%, and 18.7% in the perivascular group and 88.0%, 47.2%, 27.2%, and 22.6% in the non-perivascular group. No significant between-group differences were detected in cumulative local tumor progression (=0.567, hazard ratio: 1.224) or overall survival ( = 0.801, hazard ratio: 1.047). The major complication rate was 1.0% (1/104, > 0.999) in both groups. Tumor size was the only independent prognostic factor for local tumor progression (hazard ratio: 2.314; = 0.002). On multivariate analysis for overall colorectal liver metastases, tumor diameter >3 cm, tumor location in the right colon, multiple tumors, and extrahepatic metastases before radiofrequency ablation (hazard ratios: 2.046, 1.920, 1.706, and 1.892, respectively; all < 0.001) and intrahepatic recurrence (hazard ratio: 1.564; = 0.002) were associated with poor overall survival.

Conclusion: Cumulative local tumor progression, overall survival, and major complications rates did not differ significantly between perivascular and non-perivascular colorectal liver metastases after percutaneous radiofrequency ablation. For perivascular colorectal liver metastases, percutaneous radiofrequency ablation is a safe and effective treatment option.
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http://dx.doi.org/10.3389/fonc.2020.553556DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7596897PMC
October 2020

What is the difference in ablation zone of multi-bipolar radiofrequency ablation between liver cirrhosis and normal liver background? - a prospective clinical study.

Int J Hyperthermia 2020 ;37(1):1248-1259

Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Department of Ultrasound, Peking University Cancer Hospital and Institute, Beijing, China.

Purpose: To explore the differences in ablation zone between liver cirrhosis and normal liver background and investigate the effect of hepatic blood flow on ablation zone of RFA.

Methods: Between 2017 and 2019, 203 patients who had liver malignancies and underwent percutaneous RFA with Celon bipolar electrodes enrolled into this study. There were 90 patients had liver cirrhosis and 113 patients had normal liver background. They were 63 females and 140 males with average age of 59.0 ± 10.9 years old. Contrast-enhanced CT/MRI was used to evaluate the ablation zone in one month after RFA. The hepatic flow measurements on CDFI and CEUS were performed before RFA. Correlations between ablation zone versus hepatic flow were assessed using multiple linear regression analysis.

Results: The average ablation zone in cirrhotic liver was significantly larger than those in normal liver background with 3 cm tip of RF electrodes (length 3.5 ± 0.5 vs 3.1 ± 0.4 cm,  = 0.001; width 2.6 ± 0.3 vs 2.2 ± 0.3 cm,  < 0.001; thickness 2.5 ± 0.3 vs 2.0 ± 0.2 cm,  < 0.001). The similar result was found with three 4 cm tip of RF electrodes (width 3.6 ± 0.5 vs 3.1 ± 0.5 cm,  = 0.019; thickness 3.3 ± 0.5 vs 2.7 ± 0.5 cm,  = 0.002). The multiple linear regression analysis showed arrive time of hepatic vein and portal vein was statistically associated with ablation zone with 3 cm electrodes ( < 0.001,  = 0.001), but explained part of the variance (Adjusted R=0.294, adjusted R=0.212).

Conclusion: The ablation zones of RFA with multi-bipolar electrodes in liver cirrhosis were significantly larger than those in normal liver background, being up to 6 mm in thickness. The hepatic flow parameters partly contributed to the ablation zone.
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http://dx.doi.org/10.1080/02656736.2020.1838627DOI Listing
June 2021

[Dynamic Process of Nitrogen and Phosphorus Export and Loss Load in an Intensive Orchard with Ridge and Furrow Plantation in the Three Gorges Reservoir Area].

Huan Jing Ke Xue 2020 Aug;41(8):3646-3656

Institute of Mountain Hazards and Environment, Chinese Academy of Sciences, Chengdu 610041, China.

To comprehend the runoff load of nitrogen (N) and phosphorus (P) and the impact on the receiving river in an agricultural area with an intensive orchard plantation and a longitudinal ridge and furrow morphology in the Three Gorges Reservoir Area, the runoff and N and P concentrations were dynamically monitored in a typical citrus orchard catchment in Wanzhou Country, Chongqing, China. The results showed that the nutrient concentration in runoff water from the intensive citrus planting catchment was very high. The average annual event mean concentrations (EMC) were 9.31 mg·L for total nitrogen (TN), 8.11 mg·L for dissolved nitrogen (DN), 5.66 mg·L for nitrate nitrogen (NN), 0.51 mg·L for ammonium nitrogen (AN), 0.87 mg·L for total phosphorus, 0.56 mg·L for solved phosphorus (DP), and 0.32 mg·L for particulate phosphorus (DP). In addition, the annual loss loads were 13.43, 12.20, 8.77, 0.75, 1.26, 0.84, and 0.42 kg·(hm·a) for TN, DN, NN, AN, TP, DP, and PP, respectively. The annual average concentrations of TN and TP were 8.49 mg·L and 0.87 mg·L, respectively, which exceeded the category V values of the surface water quality standards (GB3838-2002) by 4.25 times and 2.2 times, respectively, and also exceeded the internationally recognized thresholds for the eutrophication of waterbodies. The TN and TP loss load from storm runoff was one of the main reasons for the degradation of the river water quality, thus suggesting the need to treat surface runoff and control runoff nutrient losses, especially during the first storm events after fertilization. During two typical long-duration springtime rainfall events after fertilization, the loads of nitrate nitrogen (NN) and dissolved phosphorus (DP) were 4.94 kg·hm and 0.28 kg·hm, respectively, which accounted for 92.90% and 64.69% of the total annual TN and TP loss loads, respectively. The loads of NN and DP in a short-duration high-intensity rainfall event were 0.52 and 0.05 kg·hm respectively, which accounted for 65.92% and 74.88% of the total annual TN and TP loss loads, respectively. The DN and DP were the main forms of nitrogen and phosphorus losses from the intensive citrus orchard with a longitudinal ridge and furrow morphology. Meanwhile, the catchment showed a significant first-flush phenomenon during a typical rainfall event, with a total of 58.0%, 57.0%, 58.5%, 79.0%, 62.0%, 63.5%, and 60.0% of the mass of TN, DN, NN, AN, TP, DP, and PP in the initial 20% of the runoff, respectively. Hence, controlling the surface runoff at the early runoff stage plays an important role in reducing nutrient losses.
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http://dx.doi.org/10.13227/j.hjkx.202002075DOI Listing
August 2020

The prevalence and risks of major comorbidities among inpatients with pulmonary tuberculosis in China from a gender and age perspective: a large-scale multicenter observational study.

Eur J Clin Microbiol Infect Dis 2021 Apr 22;40(4):787-800. Epub 2020 Oct 22.

Chang Chun Infectious Diseases Hospital, No. 2699, Sandao Section, Changji South Line, Erdao District, Changchun City, 130123, Jilin Province, China.

In clinical practice, PTB patients have concurrent many types of comorbidities such as pneumonia, liver disorder, diabetes mellitus, hematological disorder, and malnutrition. Detecting and treating specific comorbidities and preventing their development are important for PTB patients. However, the prevalence of most comorbid conditions in patients with PTB is not well described. We conducted a large-scale, multicenter, observational study to elucidate and illustrate the prevalence rates of major comorbidities in inpatients at 21 hospitals in China. The 19 specific comorbidities were selected for analysis in this patient cohort, and stratified the inpatient cohort according to age and gender. A total of 355,929 PTB inpatients were included, with a male:female ratio of 1.98 and the proportion of ≥ 65 years PTB inpatients was the most. Approximately 70% of PTB inpatients had at least one defined type of comorbidity. The prevalence of 19 specific comorbidities in inpatients with PTB was analyzed, with pneumonia being the most common comorbidity. The prevalence of most comorbidities was higher in males with PTB except thyroid disorders, mental health disorders, etc. The prevalence of defined most comorbidities in patients with PTB tended to increase with increasing age, although some specific comorbidities tended to increase initially then decrease with increasing age. Our study describes multiple clinically important comorbidities among PTB inpatients, and their prevalence between different gender and age groups. The results will enhance the clinical aptitude of physicians who treat patients with PTB to recognize, diagnose, and treat PTB comorbidities early.
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http://dx.doi.org/10.1007/s10096-020-04077-2DOI Listing
April 2021

Investigating Disparities by Sex and LGBTQ Identity: A Content Analysis of Sexual Health Information on College Student Health Center Websites.

J Health Commun 2020 07 19;25(7):584-593. Epub 2020 Oct 19.

Department of Communication, University of Maryland , College Park, MD, USA.

Student Health Centers (SHCs) are important resources on U.S. college campuses. In light of recent calls for creating more opportunities for health care services to young men and sexual/gender minorities, this content analytic study evaluated how sexual health information and resources are communicated on SHC websites. Utilizing a stratified random sample of 400 U.S. colleges/universities, we assessed how often sexual health is explicitly labeled for particular groups, the types of sexual health topics on SHC websites, the depth of sexual health information, and the sexual health resources offered on SHC websites. Our findings revealed that women's health webpages far outnumbered men's health webpages, sexual health topics were more common on women's health webpages, and sexual health topics were covered at greater depth on women's health webpages compared to men's health webpages. Similar disparities were found for sexual/gender minorities. General sexual health webpages on SHC websites addressed significantly more sexual health topics in greater depth and offered more sexual health resources than LGBTQ health webpages. The practical implications for college student health and potential health disparities are discussed.
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http://dx.doi.org/10.1080/10810730.2020.1825567DOI Listing
July 2020

Nanofiber-reinforced bulk hydrogel: preparation and structural, mechanical, and biological properties.

J Mater Chem B 2020 11;8(42):9794-9803

Hubei Key Laboratory of Advanced Textile Materials and Application, Hubei International Scientific and Technological Cooperation Base of Intelligent Textile Materials and Application, Wuhan Textile University, Wuhan 430200, China.

Alginate-based hydrogels are increasingly being used as biomaterials for tissue engineering, drug carriers, and wound dressing; however, their poor mechanical strength limits their applications. Nanofiber reinforcement is an effective method for increasing the mechanical strength of hydrogels. However, the macro preparation of nanofiber-reinforced hydrogels with a bulk structure is challenging. Herein, we describe the fabrication of nanofiber-reinforced bulk alginate hydrogel composites. The mechanical properties of hydrogels were significantly improved, and the reinforcement law of nanofiber was systematically studied. The maximum tensile stress (0.76 MPa) was obtained with 30% nanofiber content, which was 87% higher than that of pure alginate hydrogel. The compressive stress of the composite hydrogel exhibited "J-curve" behavior with gradually increasing nanofiber content, which indicated that the composited hydrogels were suitable as biomaterials. Furthermore, in 2 h, the hydrogels killed more than 90% of the bacteria that were present, and the bacteriostatic rate reached 100% after 12 h of treatment. More importantly, the sterile environment continued to be maintained, and the composited hydrogel also had satisfactory cytocompatibility and cell adhesion. Compared with pure alginate hydrogel, the roughness of the composited hydrogel surface was increased, which resulted in stronger cell adhesion. Therefore, the composite hydrogel demonstrated improved mechanical and biological properties, and exhibited the potential for clinical application.
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http://dx.doi.org/10.1039/d0tb01948hDOI Listing
November 2020

The Perfusion Features of Recurrent Hepatocellular Carcinoma After Radiofrequency Ablation Using Contrast-Enhanced Ultrasound and Pathological Stemness Evaluation: Compared to Initial Tumors.

Front Oncol 2020 26;10:1464. Epub 2020 Aug 26.

Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Department of Ultrasound, Peking University Cancer Hospital and Institute, Beijing, China.

To investigate the perfusion features of local recurrence in hepatocellular carcinoma (HCC) after radiofrequency ablation (RFA) with contrast-enhanced ultrasound (CEUS) and pathological correlation, as well as to compare with those of initial HCC. From 2010 to 2018, 42 patients with recurrent HCC after RFA were enrolled in this study. The initial HCC patients included 32 males and 10 females with an average age of 58.2 ± 8.1 years. The CEUS images for initial HCC lesions and local recurrence after RFA were compared. The perfusion features were analyzed, including enhancement time, process, boundary, morphology, washout time, washout degree, feeding vessels, and internal necrosis. H&E staining and CD133/EpCAM staining were performed with biopsy samples for the stemness study. According to CEUS, 59.5% of initial HCC lesions had centripetal enhancement, and 61.9% of recurrent HCC lesions had homogeneous enhancement in the arterial phase ( < 0.001). A total of 73.8% of initial HCC lesions had well-defined margins at the peak, and 81.0% of recurrent HCC lesions had poorly defined margins ( < 0.001). A total of 78.6% of initial HCC lesions had regular morphology at the peak, and 83.3% of recurrent HCC lesions were irregular ( < 0.001). Feeding vessels were more frequently found in initial HCC lesion (71.4%) than in recurrent HCCs (38.1%, = 0.002). In the late phase, 60% of initial HCCs had marked washout while 83.3% of recurrent HCC lesion had marked washout ( = 0.019). A total of 31.3% of the initial HCC lesions had internal necrosis areas while only 7.1% of recurrent HCC lesions had internal necrosis areas ( = 0.035). In tumors 3-5 cm in size, the washout time of recurrent HCCs was shorter than that of initial HCCs (50.3 ± 13.5 s vs. 75.6 ± 45.8 s, = 0.013). Pathological staining showed that the tumor stem cell markers (CD133 and EpCAM) were both highly expressed in recurrent samples compared with initial tumor samples (CD133+: 19 vs. 5%, = 0.002; EpCAM+:15 vs. 6%, = 0.005). Recurrent HCC after RFA had more homogeneous enhancement with a poorly defined border, marked washout, and fewer less feeding vessels and inner necrosis areas compared to initial HCC. The stemness study also found upregulated stemness in recurrent HCC. These specific features might be related to the aggressive biological behavior of recurrent HCC.
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http://dx.doi.org/10.3389/fonc.2020.01464DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7479182PMC
August 2020

Wire templated electrodeposition of vessel-like structured chitosan hydrogel by using a pulsed electrical signal.

Soft Matter 2020 Oct;16(41):9471-9478

Hubei Key Laboratory of Advanced Textile Materials & Application, Hubei International Scientific and Technological Cooperation Base of Intelligent Textile Materials &Application, Wuhan Textile University, Wuhan 430200, China.

Herein, by performing a templated electrodeposition process with an oscillating electrical signal stimulation, a vessel-like structured chitosan hydrogel (diameter about 0.4 mm) was successfully prepared in the absence of salt conditions. Experimental results demonstrated that the hydrogel growth (e.g. the thickness) is linearly correlated with the imposed charge transfer and can be well quantified by using a theoretical moving front model. Morphological observations indicated that the heterogeneous multilayer structure was spatially and temporally controlled by an externally employed electrical signal sequence while the channel structure could be determined by the shaped electrode. Moreover, the oscillating ON-OFF cycles were proved to strongly affect the film structure, leading to a more compact hydrogel coating with a lower water content, higher crystallinity, complex layer architecture and relatively strong mechanical properties that could be easily peeled off as a free-standing hollow tube. Importantly, all the experiments were conducted under mild conditions that allowed additional enhancing materials to be added in to further improve the mechanical and/or biological properties. Thus, this work advances a very promising self-assembly technology for the construction of a multi-functional hydrogel coating and artificial blood vessel regeneration.
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http://dx.doi.org/10.1039/d0sm01134gDOI Listing
October 2020

Silk as templates for hydroxyapatite biomineralization: A comparative study of Bombyx mori and Antheraea pernyi silkworm silks.

Int J Biol Macromol 2020 Dec 20;164:2842-2850. Epub 2020 Aug 20.

Hubei Key Laboratory of Advanced Textile Materials & Application, School of Materials Science and Engineering, Wuhan Textile University, Wuhan 430200, China. Electronic address:

Silk is extensively investigated in bone tissue engineering due to its extraordinary mechanical properties and ability to regulate biomineralization. Protein templates regulate biomineralization process through chemical interaction with ions. However, the effect of structural differences in silk fibroin on biomineralization has not been studied in detail. In this study, Antheraea pernyi silk fibroin (ASF) and Bombyx mori silk fibroin (BSF) fibers were used as templates to study the effect of silk species on biomineralization. The results showed that silk fibroin could induce the formation of calcium-deficient hydroxyapatite in simulated body fluid (SBF), and the SBF treatment resulted in the formation of silk I crystals. Compared with BSF, ASF exhibited a higher ability to induce mineralization, which may depend on the differences in hydrophilic amorphous fractions between ASF and BSF. The amorphous fractions of ASF contain more acidic amino acids, which can provide more nucleation sites in the initial stage of mineralization, resulting in faster mineralization process and more mineral deposits. This study decodes the key role of silk fibroin fractions on biomineralization, and provides deeper insights for the study of silk fibroin as biomineralization template and bone repair materials.
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http://dx.doi.org/10.1016/j.ijbiomac.2020.08.142DOI Listing
December 2020

The epidemiology of extrapulmonary tuberculosis in China: A large-scale multi-center observational study.

PLoS One 2020 21;15(8):e0237753. Epub 2020 Aug 21.

Chang Chun Infectious Diseases Hospital, Changchun City, Jilin Province, China.

Tuberculosis (TB) remains a serious global public health problem in the present. TB also affects other sites (extrapulmonary tuberculosis, EPTB), and accounts for a significant proportion of tuberculosis cases worldwide. In order to comprehensively understand epidemiology of EBTB in China, and improve early diagnosis and treatment, we conducted a large-scale multi-center observational study to assess the demographic data and the prevalence of common EPTB inpatients, and further evaluate the prevalence of EPTB concurrent with Pulmonary tuberculosis (PTB) and the associations between multiple EPTB types and gender-age group in China. All consecutive age≥15yr inpatients with a confirmed diagnosis of EPTB during the period from January 2011 to December 2017 were included in the study. The descriptive statistical analysis included median and quartile measurements for continuous variables, and frequencies and proportions with 95% confidence intervals (CIs) for categorical variables. Multinomial logistic regression analysis was used to compare the association of multiple EPTB types between age group and gender. The results showed that the proportion of 15-24 years and 25-34 years in EPTB inpatients were the most and the ratio of male: female was 1.51. Approximately 70% of EPTB inpatients were concurrent with PTB or other types of EPTB. The most common of EPTB was tuberculous pleurisy (50.15%), followed by bronchial tuberculosis (14.96%), tuberculous lymphadenitis of the neck (7.24%), tuberculous meningitis (7.23%), etc. It was found that many EPTB inpatients concurrent with PTB. The highest prevalence of EPTB concurrent with PTB was pharyngeal/laryngeal tuberculosis (91.31%), followed by bronchial tuberculosis (89.52%), tuberculosis of hilar lymph nodes (79.52%), tuberculosis of mediastinal lymph nodes (79.13%), intestinal tuberculosis (72.04%), tuberculous pleurisy (65.31%) and tuberculous meningitis (62.64%), etc. The results from EPTB concurrent with PTB suggested that females EPTB inpatients were less likely to be at higher risk of concurrent PTB (aOR = 0.819, 95%CI:0.803-0.835) after adjusted by age. As age increasing, the trend risk of concurrent PTB decreased (aOR = 0.994, 95%CI: 0.989-0.999) after adjusted by gender. Our study demonstrated that the common EPTB were tuberculous pleurisy, bronchial tuberculosis, tuberculous lymphadenitis of the neck, tuberculous meningitis, etc. A majority of patients with pharyngeal/laryngeal tuberculosis, bronchial tuberculosis, tuberculosis of hilar/mediastinal lymph nodes, intestinal tuberculosis, tuberculous pleurisy, tuberculous meningitis, etc. were concurrent with PTB. Female EPTB inpatients were less likely to be at higher risk of concurrent PTB, and as age increasing, the trend risk of concurrent PTB decreased. The clinicians should be alert to the presence of concurrent tuberculosis in EPTB, and all suspected cases of EPTB should be assessed for concomitant PTB to determine whether the case is infectious and to help for early diagnosis and treatment.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0237753PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7446809PMC
October 2020
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