Publications by authors named "Kun Shang"

53 Publications

Safety and efficacy of spleen aminopeptide oral lyophilized powder for improving quality of life and immune response in patients with advanced breast cancer: a multicenter, randomized, double-blind, placebo-controlled clinical trial.

Anticancer Drugs 2021 Jul 12. Epub 2021 Jul 12.

Department of Oncology, Beijing Friendship Hospital, Capital Medical University, Beijing, China.

Background: Health-related quality of life (HRQoL) is an important consideration in managing patients. Spleen aminopeptide oral lyophilized powder (SAOLP) has been used to enhance cellular immunity in a patient. This multicenter, randomized, double-blind, placebo-controlled clinical trial was designed to evaluate the safety and efficacy of SAOLP for improving HRQoL in patients with breast cancer.

Methods: Patients diagnosed with advanced breast cancer were included, and were administered SAOLP or placebo 4 mg qd for two cycles. The primary endpoint was improvement in HRQoL on day 42 measured by the EORTC QLQ-C30 and EORTC QLQ-BR23. Secondary endpoints included immunologic function, improvement in HRQoL on day 21 and 84, objective response rate, disease control rate, BMI and adverse events.

Result: On day 42, on the EORTC QLQ-C30 or EORTC QLQ-BR23, scores on the functional scales and QoL scale were significantly higher and scores on symptom scales were significantly lower in patients who received SAOLP compared to placebo (P < 0.05). On day 84, the number of CD3, CD4 and CD8 cells were significantly higher in patients who received SAOLP. There were no significant differences in objective response rate, disease control rate or BMI.

Conclusion: SAOLP may improve HRQoL and the immune response in patients with advanced breast cancer, represents a convenient and safe adjuvant therapy.
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http://dx.doi.org/10.1097/CAD.0000000000001109DOI Listing
July 2021

Macrocyclic Photoinitiator Based on Prism[5]arene Matching LEDs Light with Low Migration.

Macromol Rapid Commun 2021 Jun 25:e2100299. Epub 2021 Jun 25.

State Key Laboratory of Chemical Resource Engineering, Beijing Laboratory of Biomedical Materials, Changzhou Institute of Advanced Materials, Beijing University of Chemical Technology, Beijing, 100029, P. R. China.

In this work, a naphthalene-based macrocycle prism[5]arene (NP OCH ) is developed as a novel kind of photoinitiator. When NP OCH is irradiated under light, the bond between methylene and naphthalene can be quickly broken owning to the existence of ring tension. The macrocycle is cleaved to linear oligomer biradicals, which can effectively initiate the free radical photopolymerization of acrylate monomers. Compared with conventional photoinitiators, NP OCH has strong light absorption in the wavelength range of 365-405 nm, so it can well match the environment-friendly light-emitting diodes (LEDs) light source to realize highly efficient initiation. In addition, there is no small molecule fragment generated during NP OCH fracture, and the resulted linear oligomer biradicals can be immobilized in the polymer after initiating polymerization, so NP OCH photoinitiators show much lower migration rate and cytotoxicity. Cleavable macrocycle prismarene may provide a new idea for the design of safe and efficient photoinitiators matching long wavelength light.
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http://dx.doi.org/10.1002/marc.202100299DOI Listing
June 2021

Decreased expression of Dlg5 is associated with a poor prognosis and epithelial-mesenchymal transition in squamous cell lung cancer.

J Thorac Dis 2021 May;13(5):3115-3125

Department of Oncology, Beijing Friendship Hospital, Capital Medical University, Beijing, China.

Background: Discs large homolog 5 (Dlg5) is a newly discovered member of the membrane-associated guanylate kinase superfamily (MAGUK) that is involved in several important processes, including the maintenance of epithelial cell polarity, cell proliferation control, and cell migration and invasion. Decreased expression of Dlg5 has been reported in malignancies arising from different organs. In the present study, we analyzed Dlg5 expression and its prognostic value in squamous cell lung cancer (SqCLC).

Methods: Tumor tissue and adjacent normal tissue sections were collected from 98 patients with SqCLC. The expression levels of Dlg5 and epithelial-mesenchymal transition (EMT) biomarkers in the tissue sections were examined by immunohistochemistry and western blot.

Results: There were 80 males and 18 females in the study cohort. Patients at pathological stages I and IIIA accounted for 64.3% and 35.7% of the cohort, respectively. Western blot showed that Dlg5 expression differed between SqCLC and healthy tissues. Western blot also revealed low Dlg5 expression to be associated with low E-cadherin expression and high vimentin expression, which was consistent with the findings of immunohistochemical staining. Dlg5 expression was significantly correlated with lymph node (LN) metastasis (P=0.001) and disease recurrence (P<0.001), as well as with E-cadherin and vimentin expression (P=0.025 and P=0.001, respectively). Univariate analysis showed that overall survival was significantly correlated with the tumor-node-metastasis (P<0.001) and T (P=0.001) stages, LN metastasis (P<0.001), Dig5 expression (P<0.001), β-catenin expression (P=0.004), and vimentin expression (P=0.002). Patients with overexpression of Dlg5 and β-catenin had a more favorable prognosis than those without. Multivariate analysis revealed that tumor-node-metastasis stage [hazard ratio (HR) =2.124; 95% confidence interval (CI), 1.195-3.777; P=0.010], Dlg5 expression (HR =0.548; 95% CI, 0.313-0.959; P=0.035), β-catenin expression (HR =0.545; 95% CI, 0.312-0.953; P=0.033), and vimentin expression (HR =1.850; 95% CI, 1.050-3.258; P=0.033) could all independently predict the overall survival of patients with SqCLC.

Conclusions: Dlg5 is an important player in EMT which may have potential predictive value for SqCLC prognosis after surgery.
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http://dx.doi.org/10.21037/jtd-21-752DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8182517PMC
May 2021

Leojaponin inhibits NLRP3 inflammasome activation through restoration of autophagy via upregulating RAPTOR phosphorylation.

J Ethnopharmacol 2021 Oct 9;278:114322. Epub 2021 Jun 9.

Key Laboratory of Medicinal Chemistry for Natural Resource, Ministry of Education, Yunnan Provincial Center for Research & Development of Natural Products, School of Chemical Science and Technology, Yunnan University, Kunming, 650091, China. Electronic address:

Ethnopharmacological Relevance: Duan Teng Yimu decoction is a Chinese herbal medicine compound with proven therapeutic effects on inflammasome-related diseases, such as rheumatoid arthritis. This decoction consists of three Chinese herbal medicines, including Leonurus japonicus (L. japonicus), which promotes the blood circulation and exhibits detumescence activity, traditionally curing gynecologic and inflammasome diseases.

Aim Of The Study: To explore the anti-inflammasome activity and the underlying mechanisms of action of the compounds from L. japonicus.

Materials And Methods: A series of compounds were isolated from L. japonicus. Their anti-inflammasome activities were evaluated in macrophages that were co-stimulated by lipopolysaccharide (LPS) and NLRP3 inflammasome inducers. NLRP3 inflammasome formation and apoptosis speck like containing a CARD (ASC) oligomerization were evaluated by immunofluorescent microscopy and Western blot analysis. The regulation of autophagy after treatment of this compound was also evaluated. Lastly, in vivo activity of Leojaponin was analyzed in a mouse acute gouty arthritis model.

Results: Here we show that Leojaponin, a diterpenoid compound from L. japonicus, suppressed lactate dehydrogenase and IL-1β release in Nigericin-stimulated macrophages in a pyroptosis model. Leojaponin inhibits NLRP3 inflammasome activation in both J774A.1 cells and bone marrow-derived macrophages in a dose dependent manner. Moreover, Leojaponin suppressed NLRP3-mediated ASC specks formation and ASC oligomerization. These activities of Leojaponin depend on restoration of autophagy via promoting RAPTOR phosphorylation. Furthermore, Leojaponin ameliorated monosodium urate (MSU)-induced acute gouty arthritis in vivo.

Conclusion: Our findings suggest that Leojaponin inhibits NLRP3 inflammasome activation through enhancing autophagy via RAPTOR phosphorylation, thereby highlighting Leojaponin as a potent drug for inflammasome-related diseases.
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http://dx.doi.org/10.1016/j.jep.2021.114322DOI Listing
October 2021

Crossed cerebellar diaschisis: risk factors and prognostic value in focal cortical dysplasia by F-FDG PET/CT.

Ann Nucl Med 2021 Jun 11;35(6):719-727. Epub 2021 Apr 11.

Department of Nuclear Medicine, Xuanwu Hospital, Capital Medical University, No. 45 Changchun Street, Xicheng District, Beijing, 100053, China.

Purpose: Crossed cerebellar diaschisis (CCD) has been widely studied in hemispheric stroke but is less characterized in epilepsy. In this study, we used F-FDG PET/CT to investigate the risk factors for CCD and its prognostic value for intractable epilepsy caused by focal cortical dysplasia (FCD).

Methods: One hundred medically intractable epilepsy patients pathologically diagnosed with FCD postoperatively were included and classified into two groups: CCD+ and CCD-. PET/CT images were analyzed qualitatively by visual assessment and semi-quantitatively using the absolute asymmetry index (|AI|). Clinical factors, including age, sex, body mass index (BMI), age at seizure onset, epilepsy duration, seizure type, seizure frequency, electroencephalography (EEG) and brain magnetic resonance imaging (MRI), were retrospectively assessed from medical records. Follow-up outcomes were evaluated according to the Engel classification at 3, 6, 12, 24 and 36 months postoperatively.

Results: Of the 100 patients, 77 (77.0%) were classified as CCD-, and 23 (23.0%) were classified as CCD + . CCD+ patients had a higher number of lobes involved on PET (3.61 ± 2.16 vs 2.26 ± 1.01, P < 0.001) than CCD- patients. CCD- patients showed more negative MRI results than CCD + patients (P = 0.02). At 12 months postoperatively, 70.7% (29/41) of CCD- patients and 30.8% (4/13) of CCD + patients presented a favourable prognosis (P = 0.02). Significant differences in the average |AI| values in the posterior frontal and anterior temporal lobes were found between CCD+ and CCD- patients (P < 0.05).

Conclusion: The number of lobes involved on PET, structural anomalies on MRI, the |AI| values in the posterior frontal and anterior temporal lobes may be predisposing factors for CCD. CCD occurrence may help predict the prognosis of FCD patients at 12 months postoperatively.
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http://dx.doi.org/10.1007/s12149-021-01613-8DOI Listing
June 2021

Application of 2D Materials to Potassium-Ion Hybrid Capacitors.

ChemSusChem 2021 May 7;14(9):1974-1986. Epub 2021 Apr 7.

College of Medicine, Yan'an University, Yan'an, 716000, P. R. China.

Metal-ion hybrid supercapacitors (MICs) are a new type of electrochemical energy storage (EES) device, consisting of a battery-type electrode and a supercapacitor (SC)-type electrode. Exhibiting the advantages of both batteries and SCs (e. g., good energy density, excellent power density and long cycle life), these advanced energy storage devices have considerable commercial application prospects. Among MICs, potassium-ion hybrid supercapacitors (PICs) have several further advantages, including abundancy of resources, low standard electrode potential, and low cost. PICs are regarded as potential substitutes for lithium- or sodium-ion hybrid supercapacitors. However, the practical applications of PICs remain limited, owing to the imbalance of kinetics and capacity between the electrodes, the slow ion/electron diffusion rate, and the poor electrode structural stability. Recently, 2D materials with distinct structures and fascinating features have elicited widespread attention for application in PICs, thus achieving significant enhancements, ranging from charge storage capacity to reaction kinetics. This Review discusses research progress in 2D materials for PICs. Firstly, the energy storage principle and development requirements of MICs are introduced. The pivotal advantages and significant roles of 2D materials in the fabrication of PICs are then discussed in detail. Lastly, the challenges and prospects of the application of 2D materials to high-performance PICs are presented.
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http://dx.doi.org/10.1002/cssc.202100255DOI Listing
May 2021

White matter network disorder in mesial temporal epilepsy: An fMRI study.

Epilepsy Res 2021 May 18;172:106590. Epub 2021 Feb 18.

Center for MRI Research, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, China; Beijing City Key Laboratory for Medical Physics and Engineering, Institution of Heavy Ion Physics, School of Physics, Peking University, Beijing, China; McGovern Institute for Brain Research, Peking University, Beijing, China. Electronic address:

Mesial temporal lobe epilepsy (mTLE) has been considered a network disorder disease in which brain regions extending beyond the epileptogenic zones are always affected. However, abnormalities in white matter (WM) functional networks and their associations with widespread network dysfunction are still being identified in mTLE. Accordingly, we investigated the altered functional activities in WM networks in mTLE using fMRI, which has recently been used to probe WM function. We collected resting-state fMRI data from 39 unilateral mTLE patients with hippocampal sclerosis and 29 healthy controls. Eleven WM networks were clustered according to temporal correlation profile. The functional connectivity (FC) of the WM networks were evaluated and compared between the two groups. Furthermore, we assessed the capacity of WM FC for seizure lateralization. According to our analysis, mTLE led to decreased FC within deep WM networks. In addition, the cortical regions involved in seizure propagation and several brain regions displaying interhemispheric disruption showed enhanced functional coupling with deep WM networks. FCs between the ipsilateral deep WM networks and the insula, temporal lobe, and supramarginal gyrus demonstrated positive correlation with seizure frequency. Moreover, the seizure onset zones of 33 patients out of 39 patients could be correctly lateralized. Our findings reveal functional disruptions in WM networks extending to extratemporal regions, supporting the network disorder hypothesis and suggesting that deep WM networks are key network nodes associated with massive dysfunction in mTLE. Moreover, the FC of the WM represents a potentially useful functional imaging measure for the diagnosis of mTLE.
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http://dx.doi.org/10.1016/j.eplepsyres.2021.106590DOI Listing
May 2021

Assessment of localization accuracy and postsurgical prediction of simultaneous F-FDG PET/MRI in refractory epilepsy patients.

Eur Radiol 2021 Feb 27. Epub 2021 Feb 27.

Department of Nuclear Medicine, Xuanwu Hospital, Capital Medical University, Beijing, China.

Objectives: To evaluate the accuracies of simultaneous F-fluorodeoxyglucose positron emission tomography/magnetic resonance imaging ([F]-FDG PET/MRI) in preoperative localization and the postsurgical prediction.

Methods: This retrospective study was performed on ninety-eight patients diagnosed with refractory epilepsy whose presurgical evaluation included [F]-FDG PET/MRI, with 1-year post-surgery follow-up between August 2016 and December 2018. PET/MRI images were interpreted by two radiologists and a nuclear medicine physician to localize the EOZ using standard visual analysis and asymmetry index based on standard uptake value (SUV). The localization accuracy and predictive performance of simultaneous F-FDG PET/MRI based on the surgial pathology and postsurgical outcome were evaluated.

Results: A total of 41.8% (41/98) patients were found to have a definitely structural abnormality on the MR portion of PET/MRI; 93.9% (92/98) were shown hypometabolism on the PET portion of the hybrid PET/MRI. PET/MRI identified 18 cases with subtle structural abnormalities on MRI re-read. Six percent (6/98) of patients PET/MRI were negative. A total of 65.3% (64/98) patients showed seizure-free at 1-year follow-up after epilepsy surgery. The sensitivity, specificity, and accuracy of [F]-FDG PET/MRI was 95.3%, 8.8%, and 65.3% for seizure onset localization based on surgical pathology and postsurgical outcome, respectively. Multivariate regression analysis indicated that concordant of EOZ localization between PET/MRI and surgical resection range, which was a good positive predictor of seizure freedom (Engel I) (OR = 14.741, 95% CI 3.934-55.033, p < 0.001).

Conclusions: [F]-FDG PET/MRI used as two combined modalities providing additional sensitivity when detecting possible epileptic foci and will probably improve the surgical outcome.

Key Points: • Sensitivity, specificity, and accuracy of [F]-FDG PET/MRI were 95.3%, 8.8%, and 65.3% for seizure onset localization based on surgical pathology and postsurgical outcome, respectively. • Concordance of EOZ localization between PET/MRI and surgical resection range was a good positive predictor of seizure freedom; presurgical [F]-FDG PET/MRI will probably improve the surgical outcome.
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http://dx.doi.org/10.1007/s00330-021-07738-8DOI Listing
February 2021

Voxel-based morphometric MRI post-processing and PET/MRI co-registration reveal subtle abnormalities in cingulate epilepsy.

Epilepsy Res 2021 Mar 8;171:106568. Epub 2021 Feb 8.

Beijing Institute of Functional Neurosurgery, Xuanwu Hospital, Capital Medical University, Beijing, China. Electronic address:

Objective: Diagnostic challenges exist in the presurgical evaluation of patients with magnetic resonance imaging (MRI) negative cingulate epilepsy (CE) because of the heterogeneity in clinical semiology and lack of localizing findings on scalp electroencephalographic (EEG) recordings. We aimed to examine the neuroimaging characteristics in a consecutive cohort of patients with MRI-negative CE with a focus on two image post-processing methods, including the MRI post-processing morphometric analysis program (MAP) and F-fluorodeoxyglucose-positron emission tomography-MRI (PET/MRI) co-registration.

Methods: Included in this retrospective study were patients with MRI-negative CE who met the following criteria: negative on preoperative MRI, invasive EEG (iEEG) confirmed cingulate gyrus-onset seizures, surgical resection of the cingulate gyrus with/without adjacent cortex, and seizure-free for more than 12 months. MAP and PET/MRI co-registration were performed and investigated by comparison to ictal intracranial EEG findings. Other characteristics obtained from scalp EEG, magnetoencephalography (MEG), iEEG, and pathological study were also reported.

Results: Ten patients were included, of which eight were diagnosed with anterior CE, one with middle CE, and one with posterior CE. The semiology included fear, embarrassment, vocalization, ictal pouting, asymmetric tonic posture, hypermotor, and automatism. Scalp EEG revealed unilateral or bilateral frontal-temporal onset. MEG localized the dipoles correctly in one patient (1/10). MAP detected subtle abnormalities in regions concordant with iEEG onset in seven patients (7/10) while PET/MRI co-registration revealed focal concordant hypometabolism in five patients (5/10). Combining MAP with PET/MRI co-registration improved the detection rate to 90 % in this cohort. The pathology was focal cortical dysplasia (FCD), including FCD type IIA in three, type IIB in three, and type I in four.

Conclusion: MAP and PET/MRI co-registration show promising results in identifying subtle FCD abnormalities in CE with negative results on conventional MRI, which can be otherwise challenging. More importantly, a combination of MRI post-processing and PET/MRI co-registration can greatly improve the identification of epileptic abnormalities, which can be used as surgical target. MAP and PET/MRI co-registration should be incorporated into the routine presurgical evaluation.
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http://dx.doi.org/10.1016/j.eplepsyres.2021.106568DOI Listing
March 2021

Deep Vein Thrombosis in the Uninjured Lower Extremity: A Retrospective Study of 1454 Patients With Lower Extremity Fractures.

Clin Appl Thromb Hemost 2021 Jan-Dec;27:1076029620986862

Department of Orthopedic Trauma, Honghui Hospital, 12480Xi'an Jiaotong University, Beilin District, Xi'an, Shaanxi Province, People's Republic of China.

The purpose of this study was to identify patients at higher risk of deep venous thrombosis (DVT) in the uninjured lower extremity both preoperatively and postoperatively in patients with lower extremity fractures. We collected the clinical data of patients with lower extremities fractures who presented at Xi'an Honghui Hospital between 1 July, 2015 and 31 October, 2017. Doppler ultrasonography was used to diagnose the DVT. Patients were examined pre- and postoperatively. The patients were divided into thrombosis group and no thrombosis group according to the preoperative and postoperative ultrasonography results. The thrombosis group was defined as patients with DVT in the uninjured lower extremity and the no thrombosis group was defined as patients without DVT in the uninjured lower extremity. This study enrolled 1454 patients who met the inclusion criteria. The incidence of preoperative DVT in the uninjured lower extremity was 9.63% whereas the postoperative incidence was 20.29%. Age (OR = 0.965, 95 CI%: 0.954-0.977; ≤ 0.001) and female (OR = 0.667, 95% CI: 0.451-0.986, = 0.042) were independent risk factors for preoperative DVT in the uninjured lower extremity. Blood loss (OR = 0.997, 95 CI%: 0.995-1.000; = 0.020), D-dimer level at admission (OR = 0.941, 95 CI%: 0.887-0.999; = 0.045), and postoperative day 5 D-dimer level (OR = 0.889, 95 CI%: 0.819-0.965; = 0.005), were independent risk factors for postoperative DVT in the uninjured lower extremity. For the patients with lower extremity fractures, age and female were associated with the preoperative DVT in the uninjured lower extremity. Blood loss, D-dimer at admission and postoperative day 5 D-dimer were associated with the postoperative DVT in the uninjured lower extremity.
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http://dx.doi.org/10.1177/1076029620986862DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7802023PMC
January 2021

Cataract formation in transgenic HO-1 G143H mutant mice: Involvement of oxidative stress and endoplasmic reticulum stress.

Biochem Biophys Res Commun 2021 01 29;537:43-49. Epub 2020 Dec 29.

Department of Ophthalmology, The Chinese People's Liberation Army General Hospital, Beijing, China. Electronic address:

Oxidative stress and endoplasmic reticulum (ER) stress are the key contributing factors for cataract progression. In our previous studies, we demonstrated that the nuclear factor erythroid 2-like-2 (Nrf-2)/heme oxygenase-1 (HO-1)/carbon monoxide (CO) axis protects lens epithelial cells (LECs) against oxidants and ER stress. In the present study, transgenic FVB/N mice overexpressing the negative dominant mutant HO-1 G143H (TgHO-1 G143H) were generated to evaluate the crosstalk among HO-1, oxidative stress and ER stress in maintaining lens transparency. Slit-lamp examination revealed that nuclear cataracts occurred at 4 months in the TgHO-1 G143H mice, which was 5 months earlier than that of the control mice. The lenses of the transgenic mice showed an accumulation of malondialdehyde and protein carbonyl with a decrease in glutathione and protein sulfhydryl levels. Elevated concentrations of ER stress biomarkers (Bip, PERK, ATF6, IRE1, CHOP, caspase-12 and caspase-3) in the lenses of the TgHO-1 G143H mice were identified by western blotting. Furthermore, we confirmed that overexpressed HO-1 G143H in LECs resulted in oxidative insult and apoptosis in vitro. All of these data suggested that HO-1 enzymatic activity loss induces early-onset nuclear cataracts by activating oxidative stress and ER stress.
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http://dx.doi.org/10.1016/j.bbrc.2020.12.071DOI Listing
January 2021

[Expression of the kallistatin gene in rat corpus cavernosum].

Zhonghua Nan Ke Xue 2020 Mar;26(3):223-227

Department of Reproductive Medicine, The First People's Hospital of Yunnan Province, Kunming, Yunnan 650032, China.

Objective: To investigate the expression of the kallistatin gene in the corpus cavernosum and search for some new molecular targets for the regulation of penile erectile function and treatment of ED.

Methods: Using reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR), Western blot and immunofluorescence staining, we detected the expression of kallistatin in the rat corpus cavernosum and compared it with that in the aorta.

Results: The results of RT-qPCR and Western blot revealed both mRNA and protein expressions of kallistatin in the rat corpus cavernosal tissue, with no statistically significant difference from those in the aorta (P > 0.05). Immunofluorescence staining showed that kallistatin was expressed in both endothelial and smooth muscle cells in the corpus cavernosum and localized in the cytoplasm, with no statistically significant difference from its expression in the aorta (P > 0.05) either.

Conclusions: The kallistatin gene is highly expressed in the corpus cavernosum and localized in cavernosal endothelial and smooth muscle cells, suggestive of its involvement in the cellular function of cavernosal endothelial and smooth muscle cells and its participation in the regulation of penile erectile function.
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March 2020

HO/HOCl-based fluorescent probes for dynamically monitoring pathophysiological processes.

Analyst 2020 Nov;145(23):7477-7487

Shaanxi Province Key Laboratory of Catalytic Foundation and Application, College of Chemistry and Environment Science, Shaanxi University of Technology, Hanzhong 723001, China.

Serving as representative reactive oxygen species (ROS), H2O2 and HOCl play crucial roles in biological metabolism and intercellular oxidation-reduction dynamic equilibrium. The overexpression of H2O2/HOCl may cause a variety of diseases, such as acute and chronic inflammation, cancer and neurodegenerative disorders. A major question in H2O2/HOCl-based pathological diagnosis is knowing how H2O2/HOCl concentrations can be accurately regulated to initiate a diagnosis and subsequently guarantee therapeutic effects in the course of medical advances. Fluorescent probes, with their great spatial and temporal resolutions, have been used in diverse pathophysiological processes and developed rapidly in the last five years. We summarise in this review the optical properties of H2O2/HOCl-responsive fluorescent probes and focus on effective distribution and dynamic monitoring by using pathophysiological models.
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http://dx.doi.org/10.1039/d0an01313gDOI Listing
November 2020

Angiogenesis is promoted by exosomal DPP4 derived from 5-fluorouracil-resistant colon cancer cells.

Cancer Lett 2021 01 9;497:190-201. Epub 2020 Oct 9.

Department of Oncology, Beijing Friendship Hospital, Capital Medical University, Beijing, 100050, China. Electronic address:

Cancer cells can communicate with the tumor microenvironment and contribute to tumor progression. However, the effects of drug-resistant tumor cells on angiogenesis are unclear. Current anti-angiogenic strategies also have limitations and it would be useful to develop novel targets and treatment strategies. Here, our study showed that the conditioned medium and exosomes from 5-FU-resistant colon cancer cells promoted angiogenesis, and we observed that exosomal dipeptidyl peptidase IV (DPP4) was a potent inducer of this angiogenesis. DPP4-enriched exosomes increased periostin (POSTN) expression in human umbilical vein endothelial cells via Twist1 nuclear translocation or activating Smad signaling pathway, while silencing or inhibition of DPP4 neutralized those effects. The in vivo and clinical data indicated that high DPP4 expression was related to tumor progression. These findings indicate that DPP4 may be a target for inhibiting angiogenesis in 5-FU-resistant colon cancer. Furthermore, exosomal DPP4 concentrations may be a useful prognostic marker for colon cancer.
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http://dx.doi.org/10.1016/j.canlet.2020.10.009DOI Listing
January 2021

Deep Vein Thrombosis in Patients with Intertrochanteric Fracture: A Retrospective Study.

Indian J Orthop 2020 Sep 11;54(Suppl 1):101-108. Epub 2020 Jul 11.

Department of Orthopedic Trauma, Honghui Hospital, Xi'an Jiaotong University College of Medicine, Beilin District, No. 555 Youyi East Road, Xi'an, 710054 Shaanxi China.

Background: This study aimed to investigate the incidences of pre- and post-operative lower extremity deep venous thrombosis (DVT) in hospitalized patients with intertrochanteric fractures and to analyze the relevant risk factors.

Methods: A retrospective study was conducted between July 2014 and October 2016 in 218 intertrochanteric fracture patients who presented at Xi'an Honghui Hospital and underwent Doppler ultrasonography for DVT diagnosis. We divided DVT into distal, proximal, and mixed thrombosis. Patients were divided into either the thrombosis or no thrombosis group according to preoperative and postoperative ultrasonography results. All patients were evaluated for the risk factors associated with thrombosis.

Results: A total of 37.61% of preoperative patients had DVT, and the postoperative incidence increased to 58.72%. The days between fracture and hospitalization and the days between fracture and surgery were independent risk factors for preoperative DVT. The days between fracture and hospitalization and d-dimer levels at postoperative 1 day were independent risk factors of postoperative DVT. In total 23.4% of the patients progressed from having no thrombosis preoperatively to having distal, proximal, or mixed DVT postoperatively (22.02%, 0.46%, and 0.92%, respectively). Distal DVT constituted 86.59% and 90.63% of all preoperative and postoperative DVTs, respectively.

Conclusion: Intertrochanteric fracture is a common type of hip fracture in the elderly, and the incidence of DVT after intertrochanteric fracture may be underestimated. Early intervention (early admission and early surgery) might reduce the incidence of DVT.
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http://dx.doi.org/10.1007/s43465-020-00166-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7474015PMC
September 2020

The Efficacy of Teriparatide in Improving Fracture Healing in Hip Fractures: A Systematic Review and Meta-Analysis.

Biomed Res Int 2020 20;2020:5914502. Epub 2020 Aug 20.

Department of Orthopedic Trauma, Honghui Hospital, Xi'an Jiaotong University, Beilin District, Xi'an, Shaanxi Province, China.

Background: This systematic review and meta-analysis assessed the role of teriparatide in improving hip fracture healing and function to provide a clinical guide.

Methods: The systematic literature review identified randomized controlled trials (RCTs) and controlled studies evaluating teriparatide for elderly hip fractures. A meta-analysis was performed using RevMan version 5.3.

Results: This study included two RCTs and four retrospective studies comprising 607 patients, with 269 and 338 patients in the teriparatide and control groups, respectively. The quality of these six studies was moderate. Compared to the control group, teriparatide reduced the time to union (weighted mean difference (WMD) = -1.95; 95% confidence interval (CI): -3.23--0.68; = 0.003) but did not improve the rate of fracture union at 3 months (odds ratio (OR) = 1.46; 95% CI: 0.50-4.24; = 0.49) or 6 months (OR = 0.89; 95% CI: 0.44-1.81; = 0.75). In addition, teriparatide did not decrease the complications, need for reoperation, mortality, rate of deformity after fracture healing, and subsequent fracture or improve hip function.

Conclusions: The current limited evidence did not support that teriparatide improves fracture healing in hip fractures, due to study heterogeneity and various sources of biases. Further high-quality, large-sample trials are needed. This trial is registered with PROSPERO with registration number CRD42020152205.
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http://dx.doi.org/10.1155/2020/5914502DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7456478PMC
April 2021

Positive relationship of hypertensive retinopathy with carotid intima--media thickness in hypertensive patients.

J Hypertens 2020 10;38(10):2028-2035

Department of Ophthalmology, Peking University First Hospital, Beijing.

Objectives: We aimed to explore the relationship of hypertensive retinopathy with carotid intima--media thickness (CIMT), and to examine the possible effect modifiers in Chinese adults with hypertension.

Methods: We conducted a cross-sectional study of 12 342 hypertensive patients with complete exit site visit data from the China Stroke Primary Prevention Trial. CIMT was measured by carotid ultrasonography. Hypertensive retinopathy was diagnosed according to the Keith--Wagener--Barker classification.

Results: The mean (SD) CIMT among study participants was 739.9 (111.4) μm. Compared with patients with grade 1 hypertensive retinopathy or without hypertensive retinopathy, a significantly higher CIMT level (β, 7.63, 95% CI: 2.54--12.73) was observed in patients with grade 2-4 hypertensive retinopathy. Moreover, the association between hypertensive retinopathy (grade 2-4 versus grade 1 or normal) and CIMT was stronger in participants of younger age (<60 years; β, 13.70, 95% CI: 5.65--21.75; versus ≥60 years; β, 1.03, 95% CI: -5.58 to 7.63; P interaction = 0.006); or with lower total homocysteine levels [<12.1 μmol/l (median); β, 12.70, 95% CI: 5.98--19.42; versus ≥12.1 μmol/l; β, 2.07, 95% CI: -5.63 to 9.78; P interaction = 0.030). None of the other variables, including sex, BMI, study centers, treatment group, SBP, triglycerides, total cholesterol, fasting blood glucose, folate, serum creatinine, current smoking and alcohol drinking, significantly modified the relation of hypertensive retinopathy with CIMT levels.

Conclusion: Hypertensive retinopathy (grade 2 and higher) was significantly associated with increased CIMT in hypertensive patients. The association was stronger in those of younger age or with lower total homocysteine levels.
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http://dx.doi.org/10.1097/HJH.0000000000002509DOI Listing
October 2020

Altered coupling between resting-state glucose metabolism and functional activity in epilepsy.

Ann Clin Transl Neurol 2020 10 29;7(10):1831-1842. Epub 2020 Aug 29.

Department of Nuclear Medicine, Xuanwu Hospital Capital Medical University, Beijing, China.

Objective: Altered functional activities and hypometabolism have been found in medial temporal lobe epilepsy patients with hippocampal sclerosis (mTLE-HS). Hybrid PET/MR scanners provide opportunities to explore the relationship between resting-state energy consumption and functional activities, but whether repeated seizures disturb the bioenergetic coupling and its relationship with seizure outcomes remain unknown.

Methods: F-FDG PET and resting-state functional MRI (rs-fMRI) scans were performed with hybrid PET/MR in 26 patients with mTLE-HS and in healthy controls. Energy consumption was quantified by F-FDG standardized uptake value ratio(SUVR) relative to cerebellum. Spontaneous neural activities were estimated using regional homogeneity (ReHo), fractional amplitude of low frequency fluctuations (fALFF) from rs-fMRI. Between-group differences in SUVR and rs-fMRI derived metrics were evaluated by two-sample t test. Voxel-wise spatial correlations were explored between SUVR and ReHo, fALFF across gray matter and compared between groups. Furthermore, the relationships between altered fALFF/SUVR and ReHo/SUVR coupling and surgical outcomes were evaluated.

Results: Both the patients and healthy controls showed significant positive correlations between SUVR and rs-fMRI metrics. Spatial correlations between SUVR and fMRI-derived metrics across gray matter were significantly higher in patients with mTLE-HS compared with healthy controls (fALFF/SUVR, P < 0.001; ReHo/SUVR, P = 0.022). Higher fALFF/SUVR couplings were found in patients who had Engel class IA after surgery than all other (P = 0.025), while altered ReHo/SUVR couplings (P = 0.097) were not.

Conclusion: These findings demonstrated altered bioenergetic coupling across gray matter and its relationship with seizure outcomes, which may provide novel insights into pathogenesis of mTLE-HS and potential biomarkers for epilepsy surgery planning.
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http://dx.doi.org/10.1002/acn3.51168DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7545617PMC
October 2020

Deep Vein Thrombosis in the Uninjured Limb in Patients with Lower Extremity Fractures: A Retrospective Study.

Biomed Res Int 2020 22;2020:1647617. Epub 2020 Jun 22.

Department of Orthopedic Trauma, Honghui Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi Province 710054, China.

Objective: This study is aimed at investigating the incidence of deep vein thrombosis (DVT) in the uninjured limb during hospitalization and 1 month after surgery in patients with lower extremity fractures.

Methods: We collected the clinical data of patients with lower extremity fractures in Xi'an Honghui Hospital. Doppler ultrasonography was used to diagnose DVT. According to the results of ultrasonography, the patients were divided into two groups: uninjured limb with DVT group and uninjured limb without DVT group.

Results: A total of 494 patients who met all inclusion criteria were included in this study. The incidence rate of DVT in the uninjured limb was 19.84% and 18.83% during hospitalization and 1 month after surgery, respectively. Age (OR = 1.035, 95% CI: 1.013-1.059; = 0.002) and D-dimer level 1 day after surgery (OR = 1.065, 95% CI: 1.030-1.102; < 0.001) were independent risk factors for DVT during hospitalization. Similarly, age (OR = 1.045, 95% CI: 1.021-1.070; < 0.001) and D-dimer level 1 day after surgery (OR = 1.048, 95% CI: 1.014-1.083; = 0.006) were independent risk factors for DVT 1 month after surgery. During hospitalization and 1 month after surgery, 15.79% and 12.35% of patients had double lower limb thrombosis and 4.04% and 6.48% of patients had DVT in the uninjured limb only, respectively.

Conclusion: The actual incidence of DVT in the uninjured limb in patients with lower extremity fractures cannot be ignored despite the use of anticoagulants for prevention or treatment during hospitalization. We should also be aware of DVT in the uninjured limb while focusing on DVT in the injured limb.
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http://dx.doi.org/10.1155/2020/1647617DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7330622PMC
April 2021

Nonoperative treatment versus volar locking plate fixation for elderly patients with distal radial fracture: a systematic review and meta-analysis.

J Orthop Surg Res 2020 Jul 14;15(1):263. Epub 2020 Jul 14.

Department of Orthopedic Trauma, Honghui Hospital, Xi'an Jiaotong University, No. 555 Youyi East Road, Beilin District, Xi'an, 710054, Shaanxi Province, People's Republic of China.

Background: This systematic review and meta-analysis assessed the role of nonoperative treatment and volar locking plate (VLP) fixation in elderly patients with distal radial fracture.

Methods: The systematic literature review identified randomized controlled trials (RCTs) and observational studies using VLP and nonoperative treatment for distal radial fractures in the elderly. Two investigators independently extracted data and evaluated the quality of the studies. A meta-analysis was performed using RevMan version 5.3.

Results: The five RCTs and six observational studies included 585 and 604 patients in the VLP and nonoperation groups, respectively. The quality of these 11 studies was moderate. Compared to nonoperation treatment, VLP did not improve the disabilities of the arm, shoulder and hand (DASH) score (weighted mean difference [WMD] = -1.67; 95% confidence interval [CI], -3.58--0.24; P = 0.09), decrease complications (odds ratio = 1.05; 95% CI, 0.51-2.19; P = 0.89), or improve range of motion in flexion, extension, pronation, supination, and radial deviation. The VLP group had better grip strength (WMD = 10.52; 95% CI, 6.19-14.86; P < 0.0001) and radiographic assessment than the nonoperation group.

Conclusions: Although insufficient, the study evidence shows that VLP does not improve DASH scores, complications, or range of motion, but it might provide better grip strength and radiographic assessment than nonoperation treatment.
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http://dx.doi.org/10.1186/s13018-020-01734-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7362482PMC
July 2020

Perioperative Deep Vein Thrombosis in Patients With Lower Extremity Fractures: An Observational Study.

Clin Appl Thromb Hemost 2020 Jan-Dec;26:1076029620930272

Department of Orthopedic Trauma, Honghui Hospital, Xi'an Jiaotong University, Xi'an, People's Republic of China.

This study aimed to investigate deep vein thrombosis (DVT) in patients with lower extremity fractures who received anticoagulation treatment in the perioperative stage. We collected the patients' clinical data and diagnosed DVT using Doppler ultrasonography. Preoperative, postoperative, and 1-month postoperative examinations were performed. The patients were divided into thrombosis and non-thrombosis groups according to ultrasonographic findings. A total of 404 patients were included in the study. The preoperative, postoperative, and 1-month postoperative incidence rates were, respectively, 35%, 55%, and 40% for DVT and 12%, 22%, and 20% for DVT in the uninjured contralateral lower extremity. The incidence of perioperative DVT decreased over time from 223 (55%) to 161 (40%). Multivariate analysis revealed that the independent risk factors for preoperative that of DVT were age (odds ratio [OR]: 1.03; 95% CI: 1.01-1.04; = .000); postoperative that of DVT were age (OR: 1.04; 95% CI: 1.03-1.05; = .000), blood loss (OR: 1.001; 95% CI: 1.000-1.002; = .018), and American Society of Anesthesiologists classification (OR: 2.07; 95% CI: 1.16-3.72; = .014); and 1-month postoperative that of DVT were age (OR: 1.05; 95% CI: 1.03-1.07; = .000), respectively. In conclusion, the incidence of perioperative DVT decreased over time in patients who received anticoagulation treatment. Age was an important risk factor for perioperative DVT.
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http://dx.doi.org/10.1177/1076029620930272DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7427044PMC
February 2021

Long non-coding RNA H19, a novel therapeutic target for pancreatic cancer.

Mol Med 2020 04 9;26(1):30. Epub 2020 Apr 9.

Department of Oncology, Beijing Friendship Hospital, Capital Medical University, #95 Yong An Road, Beijing, Xicheng District, China.

Pancreatic ductal adenocarcinoma (PDAC) is an aggressive malignancy with high mortality, which threats peoples' health. Unfortunately, the pathogenesis of PDAC remains unclear. Recent studies have indicated that long non-coding RNAs (lncRNAs) can regulate the development and progression of malignant tumors through varying mechanisms. LncRNA H19 has a unique expression profile and can act as a sponger of specific miRNAs to regulate the pathogenic process of many diseases, including PDAC and several other types of cancers. Here, we review the research approaches to understanding the regulatory role of H19 and potential mechanisms in the progression of PDAC and other types of cancers and diseases. These studies suggest that H19 may be a novel therapeutic target for PDAC and our findings may open new revenues for scientific researches and development of valuable therapies for these diseases in the future.
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http://dx.doi.org/10.1186/s10020-020-00156-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7146949PMC
April 2020

Feasibility of anterior pelvic ring fixation alone for treating lateral compression type 1 pelvic fractures with nondisplaced complete sacral fractures: a retrospective study.

PeerJ 2020 16;8:e8743. Epub 2020 Mar 16.

Department of Orthopedic trauma, Honghui Hospital, Xi'an Jiaotong University, Xi'an, China.

Purpose: The aim of this study was to evaluate the feasibility of anterior pelvic ring fixation alone for treating lateral compression type 1 (LC-1) fractures with nondisplaced complete sacral fractures.

Methods: Patients with LC-1 type pelvic fractures with nondisplaced complete sacral fractures in the Xi'an Honghui Hospital were screened. Those who underwent surgical treatment for the anterior pelvic ring fractures and conservative treatment for the sacral factures were included in the analysis. The Majeed and Short Form-12 (SF-12) functional scores were used to evaluate these patients.

Results: Of the 123 patients enrolled, 108 (88%) responded to our enquiries regarding the outcome. The mean follow-up period was 18.37 months for the 108 patients who responded. The mean SF-12 functional score was 48.22 ± 9.68. The mean Majeed score was 83.47 ± 9.23, including 52 with excellent, 47 with good, seven with fair, two with poor outcomes. The SF-12 functional and Majeed scores were significantly higher in those aged <45 years or without lower limb injury than in those aged ≥45 years or with lower limb injury ( < 0.05).

Conclusion: Acceptable functional outcomes can be obtained for LC-1 pelvic fractures with nondisplaced complete sacral fractures by using anterior pelvic ring fixation alone.
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http://dx.doi.org/10.7717/peerj.8743DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7081779PMC
March 2020

Comparison of posterior ring fixation with combined anterior and posterior ring fixation for the treatment of lateral compression type 2 pelvic fractures.

Int Orthop 2020 06 3;44(6):1187-1193. Epub 2020 Mar 3.

Department of Orthopedic Trauma, Honghui Hospital, Xi'an Jiaotong University, No. 555 Youyi East Road, Beilin District, 710054, Xi'an, Shaanxi, People's Republic of China.

Purpose: The aim of surgical treatment for lateral compression type 2 (LC-2) pelvic fractures is to enable early mobilization and provide pain relief. Anterior and posterior ring fixation is usually performed simultaneously, with the goal of providing good biomechanical stability. However, it is unclear whether anterior ring fixation is necessary. This study therefore aimed to determine the feasibility of isolated posterior ring fixation for LC-2 pelvic fractures.

Methods: Records of patients with LC-2 pelvic fractures were extracted from a medical database. Patients who underwent posterior pelvic ring fixation alone (PR fixation group) or anterior and posterior ring fixation (APR fixation group) were included. Patients' operative characteristics, time to clinical healing, length of hospital stay, time to weight bearing, and complications were recorded at follow-up. Radiography was used to assess healing and fracture displacement. Functional outcomes were evaluated using the Majeed grading system.

Results: The PR fixation group included 44 patients, and the APR fixation group included 49 patients. Operative time, intra-operative blood loss, units of blood transfused, intra-operative fluid administered, and post-operative drainage were lower in the PR fixation group than in the APR fixation group. Length of hospital stay was also shorter in the PR fixation group than in the APR fixation group. Although the frequency (8/44) of fracture displacement in the superior ramus prior to union was high in the PR fixation group, no significant differences in time to weight bearing, time to clinical healing, or Majeed scores were found between the groups at follow-up.

Conclusions: Isolated posterior ring fixation for LC-2 pelvic fractures is feasible; patients who underwent treatment with this technique had functional outcomes similar to that of those who underwent anterior and posterior ring fixation.
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http://dx.doi.org/10.1007/s00264-020-04512-1DOI Listing
June 2020

Deep vein thrombosis in the lower extremities after femoral neck fracture: A retrospective observational study.

J Orthop Surg (Hong Kong) 2020 Jan-Apr;28(1):2309499019901172

Department of Orthopedic Trauma, Honghui Hospital, Xi'an Jiaotong University, Xi'an, People's Republic of China.

Purpose: The actual incidence of deep vein thrombosis (DVT) in femoral neck fractures is underestimated. This study aimed to investigate the incidence of DVT in the lower extremities after femoral neck fracture before and after operation.

Methods: The clinical data of patients with femoral neck fractures treated at Xi'an Honghui Hospital between July 1, 2016, and December 31, 2018, were collected. The patients were examined with ultrasonography before and after operation and divided into thrombosis and non-thrombosis groups according to their ultrasonographic results. The incidence of DVT was reported as a percentage.

Results: The incidence rates of preoperative and postoperative DVT were 32% and 56%, respectively. DVT on the uninjured side constituted 45% of all preoperative DVT and 43% of all postoperative DVT. Peripheral DVT constituted 90% and 84% of all preoperative and postoperative DVT, respectively. Diabetes was an independent risk factor of preoperative DVT. Blood loss was an independent risk factor of postoperative DVT, and open reduction and internal fixation surgical procedure was independent protective factor of postoperative DVT as compared with hemiarthroplasty and total hip replacement.

Conclusions: The incidence rates of preoperative and postoperative DVT in the patients with femoral neck fracture were high, and orthopedists should pay more attention to DVT as a complication.
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http://dx.doi.org/10.1177/2309499019901172DOI Listing
September 2020

Site-specific incorporation of sodium tripolyphosphate into myofibrillar protein from mantis shrimp (Oratosquilla oratoria) promotes protein crosslinking and gel network formation.

Food Chem 2020 May 26;312:126113. Epub 2019 Dec 26.

College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China.

Formation of protein gels in processed muscle foods is one of the most important functionalities. To explore the mechanisms responsible for affecting gel properties of muscle proteins by phosphates, myofibrillar protein (MP) from mantis shrimp (Oratosquilla oratoria) was treated with sodium tripolyphosphate at three pH values (7.0, 8.0, and 9.0). FTIR and UPLC-MS/MS firstly confirmed that phosphate groups were introduced to MP through COP bonds via serine and threonine residues. The incorporation of STP caused increased electronegativity and solubility, more stable α-helix secondary conformation, and reduced tryptophan fluorescence intensity of MP, especially at pH 8.0 and 9.0. These changes led to a finer, ordered and denser three-dimensional gel network microstructure with higher gel strength and elasticity, and water-holding capacity. This study demonstrated that the introduction of phosphate groups could increase negatively charged residues in MP, enhance the crosslinks of proteins through ionic interaction, and promote gel network formation.
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http://dx.doi.org/10.1016/j.foodchem.2019.126113DOI Listing
May 2020

Clerodane diterpenoids with potential anti-inflammatory activity from the leaves and twigs of Callicarpa cathayana.

Chin J Nat Med 2019 Dec;17(12):953-962

Key Laboratory of Medicinal Chemistry for Natural Resource, Ministry of Education and Yunnan Province, School of Chemical Science and Technology, Yunnan University, Kunming 650091, China. Electronic address:

Phytochemical investigation of the leaves and twigs of Callicarpa cathayana led to the isolation of six new clerodane diterpenoids, cathayanalactones A-F (1-6), together with seven analogues (7-13). Their structures were established by extensive NMR analyses together with experimental and calculated ECD spectra analyses. Compounds 1, 2, 3, 7 and 11 showed inhibitory activities on lipopolysaccharide-induced nitric oxide production in RAW264.7 cells.
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http://dx.doi.org/10.1016/S1875-5364(19)30118-9DOI Listing
December 2019

Complete Genome Sequence of Strain YCSC6, a Marine Bacterium Isolated from Saturated Saltpan with Activity Against Uronema marinum.

Curr Microbiol 2020 Jan 6;77(1):129-135. Epub 2019 Nov 6.

First Institute of Oceanography, MNR, Qingdao, 266061, People's Republic of China.

Salinivibrio proteolyticus strain YCSC6 was isolated from a saturated saltpan and demonstrated to have strong insecticidal activity against turbot's pathogenic ciliate-Uronema marinum. In this study, we sequenced its complete genome. Results showed that it consists of two circular chromosomes: 2.49 Mbps and 0.74 Mbps, respectively. It encodes 3429 protein-coding sequences. Biosynthetic gene clusters predicted to synthesize bacteriocins and antimicrobial peptides were discovered, which might be the key factors to lyse and kill U. marinum. The complete genome sequence of strain YCSC6 provides insights into the fundamental genetic potential for elucidating its insecticidal mechanism against U. marinum.
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http://dx.doi.org/10.1007/s00284-019-01796-4DOI Listing
January 2020

Fabrication of Carbohydrate Chips Based on Polydopamine for Real-Time Determination of Carbohydrate⁻Lectin Interactions by QCM Biosensor.

Polymers (Basel) 2018 Nov 16;10(11). Epub 2018 Nov 16.

Shaanxi Key Laboratory of Natural Products & Chemical Biology, College of Chemistry & Pharmacy, Northwest A&F University, Yangling 712100, China.

A novel approach for preparing carbohydrate chips based on polydopamine (PDA) surface to study carbohydrate⁻lectin interactions by quartz crystal microbalance (QCM) biosensor instrument has been developed. The amino-carbohydrates were immobilized on PDA-coated quartz crystals via Schiff base reaction and/or Michael addition reaction. The resulting carbohydrate-chips were applied to QCM biosensor instrument with flow-through system for real-time detection of lectin⁻carbohydrate interactions. A series of plant lectins, including wheat germ agglutinin (WGA), concanavalin A (Con A), agglutinin I (UEA-I), soybean agglutinin (SBA), and peanut agglutinin (PNA), were evaluated for the binding to different kinds of carbohydrate chips. Clearly, the results show that the predicted lectin selectively binds to the carbohydrates, which demonstrates the applicability of the approach. Furthermore, the kinetics of the interactions between Con A and mannose, WGA and -Acetylglucosamine were studied, respectively. This study provides an efficient approach to preparing carbohydrate chips based on PDA for the lectin⁻carbohydrate interactions study.
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http://dx.doi.org/10.3390/polym10111275DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6401853PMC
November 2018

Association Between Folic Acid Supplementation and Retinal Atherosclerosis in Chinese Adults With Hypertension Complicated by Diabetes Mellitus.

Front Pharmacol 2018 30;9:1159. Epub 2018 Oct 30.

Peking University First Hospital, Beijing, China.

: This cross-section investigation included 2,199 participants with hypertension complicated by diabetes mellitus, a cohort of the China Stroke Primary Prevention Trial in which 20,702 patients with essential hypertension were given enalapril with folic acid or enalapril-only double-blind treatment for 5 years. This study aimed to explore the correlation between folic acid supplementation and retinal atherosclerosis (RA) in adults with hypertension complicated by diabetes mellitus. The diagnosis of RA was determined by non-mydriatic fundus photography and classified by the Keith-Wagener-Barker system. The statistical correlation of folic acid supplementation with RA prevalence and severity was assessed. Of our cohort, 1,698 (77.6%) participants were diagnosed with RA, and the prevalence in males and females was 78.0 and 75.6%, respectively. Participants in the enalapril group had higher total homocysteine (tHcy) levels than those in enalapril-folic acid group. Compared with the enalapril group in the tHcy > 15 μmol/L group of females, the odds ratio for the enalapril-folic acid group was 0.28 (95% confidence interval, 0.11-0.67, = 0.0061). The prevalence of RA was high (77.6%) in our cohort of adults with hypertension complicated by diabetes mellitus. Folic acid supplementation was significantly associated with reduced risk of RA in females with hyperhomocysteinemia. No significant association were seen in males.
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http://dx.doi.org/10.3389/fphar.2018.01159DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6218923PMC
October 2018
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