Publications by authors named "Kun Qin"

93 Publications

Disruption of the white matter structural network and its correlation with baseline progression rate in patients with sporadic amyotrophic lateral sclerosis.

Transl Neurodegener 2021 09 13;10(1):35. Epub 2021 Sep 13.

Huaxi MR Research Center (HMRRC), Department of Radiology, Functional and Molecular Imaging Key Laboratory of Sichuan Province, West China Hospital of Sichuan University, Chengdu, 610000, China.

Objective: There is increasing evidence that amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disease impacting large-scale brain networks. However, it is still unclear which structural networks are associated with the disease and whether the network connectomics are associated with disease progression. This study was aimed to characterize the network abnormalities in ALS and to identify the network-based biomarkers that predict the ALS baseline progression rate.

Methods: Magnetic resonance imaging was performed on 73 patients with sporadic ALS and 100 healthy participants to acquire diffusion-weighted magnetic resonance images and construct white matter (WM) networks using tractography methods. The global and regional network properties were compared between ALS and healthy subjects. The single-subject WM network matrices of patients were used to predict the ALS baseline progression rate using machine learning algorithms.

Results: Compared with the healthy participants, the patients with ALS showed significantly decreased clustering coefficient C (P = 0.0034, t = 2.98), normalized clustering coefficient γ (P = 0.039, t = 2.08), and small-worldness σ (P = 0.038, t = 2.10) at the global network level. The patients also showed decreased regional centralities in motor and non-motor systems including the frontal, temporal and subcortical regions. Using the single-subject structural connection matrix, our classification model could distinguish patients with fast versus slow progression rate with an average accuracy of 85%.

Conclusion: Disruption of the WM structural networks in ALS is indicated by weaker small-worldness and disturbances in regions outside of the motor systems, extending the classical pathophysiological understanding of ALS as a motor disorder. The individual WM structural network matrices of ALS patients are potential neuroimaging biomarkers for the baseline disease progression in clinical practice.
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http://dx.doi.org/10.1186/s40035-021-00255-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8436442PMC
September 2021

Combining Deep Learning and Graph-Theoretic Brain Features to Detect Posttraumatic Stress Disorder at the Individual Level.

Diagnostics (Basel) 2021 Aug 5;11(8). Epub 2021 Aug 5.

Huaxi MR Research Center (HMRRC), Department of Radiology, West China Hospital of Sichuan University, Chengdu 610041, China.

Previous studies using resting-state functional MRI (rs-fMRI) have revealed alterations in graphical metrics in groups of individuals with posttraumatic stress disorder (PTSD). To explore the ability of graph measures to diagnose PTSD and capture its essential features in individual patients, we used a deep learning (DL) model based on a graph-theoretic approach to discriminate PTSD from trauma-exposed non-PTSD at the individual level and to identify its most discriminant features. Our study was performed on rs-fMRI data from 91 individuals with PTSD and 126 trauma-exposed non-PTSD patients. To evaluate our DL method, we used the traditional support vector machine (SVM) classifier as a reference. Our results showed that the proposed DL model allowed single-subject discrimination of PTSD and trauma-exposed non-PTSD individuals with higher accuracy (average: 80%) than the traditional SVM (average: 57.7%). The top 10 DL features were identified within the default mode, central executive, and salience networks; the first two of these networks were also identified in the SVM classification. We also found that nodal efficiency in the left fusiform gyrus was negatively correlated with the Clinician Administered PTSD Scale score. These findings demonstrate that DL based on graphical features is a promising method for assisting in the diagnosis of PTSD.
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http://dx.doi.org/10.3390/diagnostics11081416DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8391111PMC
August 2021

Topologically convergent and divergent morphological gray matter networks in early-stage Parkinson's disease with and without mild cognitive impairment.

Hum Brain Mapp 2021 Oct 28;42(15):5101-5112. Epub 2021 Jul 28.

Huaxi MR Research Center (HMRRC), Department of Radiology, West China Hospital of Sichuan University, Chengdu, Sichuan, China.

Patients with Parkinson's disease with mild cognitive impairment (PD-M) progress to dementia more frequently than those with normal cognition (PD-N), but the underlying neurobiology remains unclear. This study aimed to define the specific morphological brain network alterations in PD-M, and explore their potential diagnostic value. Twenty-four PD-M patients, 17 PD-N patients, and 29 healthy controls (HC) underwent a structural MRI scan. Similarity between interregional gray matter volume distributions was used to construct individual morphological brain networks. These were analyzed using graph theory and network-based statistics (NBS), and their relationship to neuropsychological tests was assessed. Support vector machine (SVM) was used to perform individual classification. Globally, compared with HC, PD-M showed increased local efficiency (p = .001) in their morphological networks, while PD-N showed decreased normalized path length (p = .008). Locally, similar nodal deficits were found in the rectus and lingual gyrus, and cerebellum of both PD groups relative to HC; additionally in PD-M nodal deficits involved several frontal and parietal regions, correlated with cognitive scores. NBS found that similar connections were involved in the default mode and cerebellar networks of both PD groups (to a greater extent in PD-M), while PD-M, but not PD-N, showed altered connections involving the frontoparietal network. Using connections identified by NBS, SVM allowed discrimination with high accuracy between PD-N and HC (90%), PD-M and HC (85%), and between the two PD groups (65%). These results suggest that default mode and cerebellar disruption characterizes PD, more so in PD-M, whereas frontoparietal disruption has diagnostic potential.
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http://dx.doi.org/10.1002/hbm.25606DOI Listing
October 2021

Effect of Thermal Degradation of FKM on Three-Body Abrasion under Dry Sliding: Severe Damage Led by the Particle Detention.

Materials (Basel) 2021 Jul 8;14(14). Epub 2021 Jul 8.

Key Laboratory on Deep Geo-Drilling Technology of the Ministry of Natural Resources, School of Engineering and Technology, China University of Geosciences, Beijing 100083, China.

Both the high temperature and particle environment at the downhole greatly aggravate the abrasive wear and shorten the service life of the fluororubber (FKM) seal seriously in drilling engineering. At present, there is less awareness of the tribological behavior of seals in such complex working conditions. In this work, the abrasive wear performance of the thermally degraded FKM seal was tested in the form of simulating the intrusion of abrasive particles into the interface. Results show that the wear of both rubber seals and metal counterparts is exacerbated. Through the analysis of the wear scar morphology and friction coefficient, it is revealed that more abrasive caves scatter on the surface due to the mechanical degradation of the FKM. These abrasion caves reduce the tendency of particles to escape from the caves and prolong the abrasive action. Furthermore, the abrasion cave alters the particle motion from sliding to rolling, which leads to more caves generated on the surface of the hard tribo-pair. These results enhance the understanding of the abrasive wear for FKM seals and hopefully contribute to the promotion of seals used in hot abrasive particle environments.
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http://dx.doi.org/10.3390/ma14143820DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8307247PMC
July 2021

Annular papules in the perineal area: Porokeratosis.

Authors:
Kun Qin Lei Yu

Indian J Dermatol Venereol Leprol 2021 Jun 21. Epub 2021 Jun 21.

Department of Dermatology, Zhujiang Hospital of Southern Medical University, Guangzhou, China.

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http://dx.doi.org/10.25259/IJDVL_1359_20DOI Listing
June 2021

Endoscopic Endonasal Approach for Clipping Anterior Communicating Artery Aneurysms From Cadaver Studies and Three-Dimensional Printed Models to a Clinical Case.

J Craniofac Surg 2021 Jul 7. Epub 2021 Jul 7.

Department of Neurosurgery, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences Department of Neurology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China.

Objectives: Anterior communicating artery (ACoA) aneurysm is one of the most common intracranial aneurysms, and it is also the aneurysm with the highest rupture rate. With the improvement of endoscopic techniques, it is possible to use an endoscopic endonasal approach (EEA) to clip ACoA aneurysms. For further analysis of the EEA for clipping ACoA aneurysms, we used cadaver heads and three-dimensional (3D)-printed models to finish the anatomical study, and we finally selected 1 clinical case to complete the clipping through the EEA.

Materials And Methods: We first collected 3 cadaver heads to simulate the EEA. Then, the imaging data of 29 real cases of ACoA aneurysm were collected, and the model of an aneurysm was prepared by 3D printing technology; then, the EEA was used to simulate the clipping of the aneurysm model. Finally, a clinical case with 2 ACoA aneurysms was selected to adopt the EEA for clipping.

Results: Both the cadaver head and 3D-printed aneurysm model could simulate aneurysm clipping with the EEA. The clinical case of the selected ACoA aneurysm can successfully complete the clipping through the EEA.

Conclusions: 3D-printed models are a good method to study the anatomical characteristics of a surgical approach. For specially selected ACoA aneurysms, the EEA is relatively simple method that can be used to clip the aneurysm successfully. The EEA for clipping ACoA aneurysms is a useful complement to the current traditional craniotomy approaches and endovascular embolization.
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http://dx.doi.org/10.1097/SCS.0000000000007848DOI Listing
July 2021

Local Semantic Enhanced ConvNet for Aerial Scene Recognition.

IEEE Trans Image Process 2021 16;30:6498-6511. Epub 2021 Jul 16.

Aerial scene recognition is challenging due to the complicated object distribution and spatial arrangement in a large-scale aerial image. Recent studies attempt to explore the local semantic representation capability of deep learning models, but how to exactly perceive the key local regions remains to be handled. In this paper, we present a local semantic enhanced ConvNet (LSE-Net) for aerial scene recognition, which mimics the human visual perception of key local regions in aerial scenes, in the hope of building a discriminative local semantic representation. Our LSE-Net consists of a context enhanced convolutional feature extractor, a local semantic perception module and a classification layer. Firstly, we design a multi-scale dilated convolution operators to fuse multi-level and multi-scale convolutional features in a trainable manner in order to fully receive the local feature responses in an aerial scene. Then, these features are fed into our two-branch local semantic perception module. In this module, we design a context-aware class peak response (CACPR) measurement to precisely depict the visual impulse of key local regions and the corresponding context information. Also, a spatial attention weight matrix is extracted to describe the importance of each key local region for the aerial scene. Finally, the refined class confidence maps are fed into the classification layer. Exhaustive experiments on three aerial scene classification benchmarks indicate that our LSE-Net achieves the state-of-the-art performance, which validates the effectiveness of our local semantic perception module and CACPR measurement.
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http://dx.doi.org/10.1109/TIP.2021.3092816DOI Listing
July 2021

Regional gray matter volume associated with exercise dependence: A voxel-based morphometry study.

Hum Brain Mapp 2021 Oct 8;42(15):4857-4868. Epub 2021 Jul 8.

Huaxi MR Research Center (HMRRC), Department of Radiology, West China Hospital of Sichuan University, Chengdu, China.

Although regular physical exercise has multiple positive benefits for the general population, excessive exercise may lead to exercise dependence (EXD), which is harmful to one's physical and mental health. Increasing evidence suggests that stress is a potential risk factor for the onset and development of EXD. However, little is known about the neural substrates of EXD and the underlying neuropsychological mechanism by which stress affects EXD. Herein, we investigate these issues in 86 individuals who exercise regularly by estimating their cortical gray matter volume (GMV) utilizing a voxel-based morphometry method based on structural magnetic resonance imaging. Whole-brain correlation analyses and prediction analyses showed negative relationships between EXD and GMV of the right orbitofrontal cortex (OFC), left subgenual cingulate gyrus (sgCG), and left inferior parietal lobe (IPL). Furthermore, mediation analyses found that the GMV of the right OFC was an important mediator between stress and EXD. Importantly, these results remained significant even when adjusting for sex, age, body mass index, family socioeconomic status, general intelligence and total intracranial volume, as well as depression and anxiety. Collectively, the results of the present study provide crucial evidence of the neuroanatomical basis of EXD and reveal a potential neuropsychological pathway in predicting EXD in which GMV mediates the relationship between stress and EXD.
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http://dx.doi.org/10.1002/hbm.25585DOI Listing
October 2021

A comparative analysis of the endoscopic endonasal and pterional approaches for clipping anterior communicating artery aneurysms on three-dimensional printed models.

Chin Med J (Engl) 2021 Jun 30;134(17):2113-2115. Epub 2021 Jun 30.

Department of Neurosurgery, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong 510080, China.

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http://dx.doi.org/10.1097/CM9.0000000000001593DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8439996PMC
June 2021

Antigenic Drift of the Hemagglutinin from an Influenza A (H1N1) pdm09 Clinical Isolate Increases its Pathogenicity In Vitro.

Virol Sin 2021 Jun 9. Epub 2021 Jun 9.

State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510180, China.

The influenza A (H1N1) pdm09 virus emerged in 2009 and has been continuously circulating in humans for over ten years. Here, we analyzed a clinical influenza A (H1N1) pdm09-infected patient case hospitalized for two months in Guangdong (from December 14, 2019 to February 15, 2020). This isolate, named A/Guangdong/LCF/2019 (LCF/19), was genetically sequenced, rescued by reverse genetics, and phylogenetically analyzed in the context of other relevant pdm09 isolates. Compared with earlier isolates, this pdm09 virus's genetic sequence contains four substitutions, S186P, T188I, D190A, and Q192E, of the hemagglutinin (HA) segment at position 186-192 (H3 numbering) in the epitope Sb, and two of which are located at the 190-helix. Phylogenetic analysis indicated that the epitope Sb started undergoing a rapid antigenic change in 2018. To characterize the pathogenicity of this novel substitution motif, a panel of reassortant viruses containing the LCF/2019 HA segment or the chimeric HA segment with the four substitutions were rescued. Kinetic growth data revealed that the reassortant viruses, including the LCF/2019 with the PTIAAQE substitution, propagated faster than those rescued ones having the STTADQQ motif in the epitope Sb in Madin-Darby Canine Kidney (MDCK) cells. The HI test showed that the binding activity of escape mutant to 2018 pdm09 sera was weaker than GLW/2018, suggesting that old vaccines might not effectively protect people from infection. Due to the difference in the selection of vaccine strains, people vaccinated in the southern hemisphere could still suffer a severe infection if infected with this antigenic drift pdm09 virus.
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http://dx.doi.org/10.1007/s12250-021-00401-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8188537PMC
June 2021

Divergent Anatomical Correlates and Functional Network Connectivity Patterns in Temporal Lobe Epilepsy with and Without Depression.

Brain Topogr 2021 07 10;34(4):525-536. Epub 2021 May 10.

Department of Neurology, West China Hospital, Sichuan University, Chengdu, Sichuan, China.

Epilepsy and depression were proposed to facilitate each other reciprocally through common neurobiological anomalies, especially the prefrontal-limbic-subcortical abnormalities. Yet neuroimaging patterns of higher-order cognitive networks and neuroanatomical correlates were rarely compared in temporal lobe epilepsy patients with (TLE-D) and without depression (TLE-N). We collected T1-weighted structural and resting-state functional MRI data from 20 TLE-D, 31 TLE-N and 20 healthy controls (HCs) and performed analyses including hippocampal volume (HCV), cortical thickness, gray matter volume (GMV) and whole-brain functional network connectivity (FNC) across three groups. Imaging differences were related to clinical and psychological measurements. TLE-D demonstrated disrupted functional role of subcortical (SUB) and higher-order cognitive networks compared to TLE-N and HCs. In TLE-D, GMV in the right supplementary motor area (SMA) and FNC between the dorsal attention (DAN) and SUB were attenuated compared to TLE-N and HCs, FNC between SUB and the visual network (VIS) decreased compared to HCs. GMV in the right SMA was negatively correlated with depression severity and some symptoms. Combined, explicit emotion regulation may be impaired in TLE-D. Meanwhile, compared to HCs, TLE-N showed smaller HCVs, TLE-D and TLE-N showed smaller GMV in the medial orbital frontal gyrus and right hippocampus and hippocampal gyrus, possibly implying predisposition of epileptic activities to co-morbid depression. Our findings suggest distinct anatomical and FNC patterns in TLE-D and TLE-N. More than prefrontal-limbic-subcortical anomalies, disrupted higher-order cognitive network may contribute to depression in TLE, providing new potential treatment targets for depression and calling attention to relation between cognitive dysfunction and co-morbid depression.
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http://dx.doi.org/10.1007/s10548-021-00848-yDOI Listing
July 2021

Network-level functional topological changes after mindfulness-based cognitive therapy in mood dysregulated adolescents at familial risk for bipolar disorder: a pilot study.

BMC Psychiatry 2021 04 28;21(1):213. Epub 2021 Apr 28.

Department of Psychiatry and Behavioral Neuroscience, University of Cincinnati College of Medicine, Cincinnati, OH, USA.

Background: Given that psychopharmacological approaches routinely used to treat mood-related problems may result in adverse outcomes in mood dysregulated adolescents at familial risk for bipolar disorder (BD), Mindfulness-Based Cognitive Therapy for Children (MBCT-C) provides an alternative effective and safe option. However, little is known about the brain mechanisms of beneficial outcomes from this intervention. Herein, we aimed to investigate the network-level neurofunctional effects of MBCT-C in mood dysregulated adolescents.

Methods: Ten mood dysregulated adolescents at familial risk for BD underwent a 12-week MBCT-C intervention. Resting-state functional magnetic resonance imaging (fMRI) was performed prior to and following MBCT-C. Topological metrics of three intrinsic functional networks (default mode network (DMN), fronto-parietal network (FPN) and cingulo-opercular network (CON)) were investigated respectively using graph theory analysis.

Results: Following MBCT-C, mood dysregulated adolescents showed increased global efficiency and decreased characteristic path length within both CON and FPN. Enhanced functional connectivity strength of frontal and limbic areas were identified within the DMN and CON. Moreover, change in characteristic path length within the CON was suggested to be significantly related to change in the Emotion Regulation Checklist score.

Conclusions: 12-week MBCT-C treatment in mood dysregulated adolescents at familial risk for BD yield network-level neurofunctional effects within the FPN and CON, suggesting enhanced functional integration of the dual-network. Decreased characteristic path length of the CON may be associated with the improvement of emotion regulation following mindfulness training. However, current findings derived from small sample size should be interpreted with caution. Future randomized controlled trials including larger samples are critical to validate our findings.
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http://dx.doi.org/10.1186/s12888-021-03211-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8080341PMC
April 2021

Circular RNA circ-ARFIP2 regulates proliferation, migration and invasion in human vascular smooth muscle cells via miR-338-3p-dependent modulation of KDR.

Metab Brain Dis 2021 08 10;36(6):1277-1288. Epub 2021 Apr 10.

Department of Neurosurgery, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, No.106 Zhongshan Second Road, Guangzhou, 510000, Guangdong, China.

Dysfunction of vascular smooth muscle cells (VSMCs) plays a critical role in the pathogenesis of intracranial aneurysm (IA). Circular RNAs (circRNAs) have been implicated in the pathogenesis of IA by reducing microRNA (miRNA) activity. In this paper, we investigated the precise roles of circRNA ADP ribosylation factor interacting protein 2 (circ-ARFIP2, circ_0021001) in VSMC dysfunction. The levels of circ-ARFIP2, miR-338-3p and kinase insert domain receptor (KDR) were detected by quantitative real-time polymerase chain reaction (qRT-PCR) or western blot. Ribonuclease (RNase) R and subcellular fractionation assays were used to assess the stability and localization of circ-ARFIP2, respectively. Cell viability was detected by Cell Counting Kit-8 (CCK-8) assay, and cell invasion was measured by transwell assay. Cell proliferation was gauged by 5-Ethynyl-2'-Deoxyuridine (EdU) assay. Cell migration was evaluated by transwell and wound-healing assays. Targeted correlations among circ-ARFIP2, miR-338-3p and KDR were validated by dual-luciferase reporter and RNA immunoprecipitation (RIP) assays. Circ-ARFIP2 and KDR were underexpressed and miR-338-3p was overexpressed in the arterial wall tissues of IA patients. Overexpression of circ-ARFIP2 in human umbilical artery smooth muscle cells (HUASMCs) showed a significant promotion in cell proliferation, migration and invasion. Mechanistically, circ-ARFIP2 targeted miR-338-3p, and circ-ARFIP2 regulated cell behaviors by miR-338-3p. KDR was a direct and functional target of miR-338-3p. Moreover, KDR was a downstream effector of circ-ARFIP2 function. Circ-ARFIP2 regulated KDR expression by targeting miR-338-3p. Our present findings demonstrated that the increased level of circ-ARFIP2 enhanced HUASMC proliferation, migration and invasion at least in part by the miR-338-3p/KDR axis.
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http://dx.doi.org/10.1007/s11011-021-00726-3DOI Listing
August 2021

Changes in the brain structural connectome after a prospective randomized clinical trial of lithium and quetiapine treatment in youth with bipolar disorder.

Neuropsychopharmacology 2021 06 22;46(7):1315-1323. Epub 2021 Mar 22.

Department of Psychiatry and Behavioral Neuroscience, University of Cincinnati College of Medicine, Cincinnati, OH, USA.

The goals of the current study were to determine whether topological organization of brain structural networks is altered in youth with bipolar disorder, whether such alterations predict treatment outcomes, and whether they are normalized by treatment. Youth with bipolar disorder were randomized to double-blind treatment with quetiapine or lithium and assessed weekly. High-resolution MRI images were collected from children and adolescents with bipolar disorder who were experiencing a mixed or manic episode (n = 100) and healthy youth (n = 63). Brain networks were constructed based on the similarity of morphological features across regions and analyzed using graph theory approaches. We tested for pretreatment anatomical differences between bipolar and healthy youth and for changes in neuroanatomic network metrics following treatment in the youth with bipolar disorder. Youth with bipolar disorder showed significantly increased clustering coefficient (C) (p = 0.009) and characteristic path length (L) (p = 0.04) at baseline, and altered nodal centralities in insula, inferior frontal gyrus, and supplementary motor area. C, L, and nodal centrality of the insula exhibited normalization in patients following treatment. Changes in these neuroanatomic parameters were correlated with improvement in manic symptoms but did not differ between the two drug therapies. Baseline structural network matrices significantly differentiated medication responders and non-responders with 80% accuracy. These findings demonstrate that both global and nodal structural network features are altered in early course bipolar disorder, and that pretreatment alterations in neuroanatomic features predicted treatment outcome and were reduced by treatment. Similar connectome normalization with lithium and quetiapine suggests that the connectome changes are a downstream effect of both therapies that is related to their clinical efficacy.
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http://dx.doi.org/10.1038/s41386-021-00989-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8134458PMC
June 2021

sp. nov., isolated from Antarctic tundra soil, and emended descriptions of , , and .

Int J Syst Evol Microbiol 2019 Jun 16;71(3). Epub 2021 Mar 16.

China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, PR China.

A rod-shaped, yellow-pigmented, Gram-stain-negative, non-motile and aerobic bacterium, designated 7-3A, was isolated from soil from King George Island, maritime Antarctica, and subjected to a polyphasic taxonomic study. Growth occurred at 4-37 °C (optimum, 20°C) and at pH 5.0-9.0 (optimum, pH 7.0-8.0). Tolerance to NaCl was up to 4 % (w/v) with optimum growth in the absence of NaCl. The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 7-3A represented a member of the family . Strain 7-3A showed the highest sequence similarities with HMD 1043 (96.65 %), NCTC 13525 (96.53 %), DSM 23145 (96.27 %), LMG 24720 (96.13 %) and DSM 17048 (96.06 %). A whole genome-level comparison of 7-3A with DSM 17048, LMG 24720, DSM 23145, and DSM 21579 revealed average nucleotide identity (ANI) values of 79.03, 82.25, 78.12, and 74.42 %, respectively. The major respiratory isoprenoid quinone was identified as MK-6 and a few ubiquinones Q-10 were identified. In addition, flexirubin-type pigments were absent. The polar lipid profile of 7-3A was found to contain one phosphatidylethanolamine, six unidentified aminolipids (AL) and two unidentified lipids (L). The G+C content of the genomic DNA was determined to be 34.54 mol%. The main fatty acids were iso-C, summed feature 9 (comprising iso-Cω9 and/or C 10-methyl), anteiso-C, iso-C and summed feature 3 (comprising Cω7 and/or Cω6). On the basis of the evidence presented in this study, a novel species of the genus , sp. nov., is proposed, with the type strain 7-3A (=CCTCC AB 2016141= KCTC 52492). Emended descriptions of , , and are also given.
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http://dx.doi.org/10.1099/ijsem.0.004740DOI Listing
June 2019

sp. nov., isolated from a soil sample of Collins Glacier front, Antarctica.

Int J Syst Evol Microbiol 2019 Jun 16;71(3). Epub 2021 Mar 16.

China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, PR China.

A Gram-stain-negative, rod-shaped, and aerobic bacterium, strain 16-35-5, was isolated from Collins Glacier front soil from the Fildes Peninsula, Antarctica. The bacterium grew optimally at 28 °C, pH 7.0 and in the presence of 0-4.0 % (w/v) NaCl. On the basis of the results of 16S rRNA gene sequence phylogenetic analyses, it was concluded that 16-35-5 represented a member of the genus and had the highest sequence similarities with CFBP 3232 (96.48 %). The genome of 16-35-5 is 4.2 Mb long with a DNA G+C content of 66.3 mol%. Average nucleotide identity (ANI) value between the genomes of 16-35-5 and DSM 27981, was 85.29 %. Strain 16-35-5 had ubiquinone-8 (Q-8) as the respiratory ubiquinone. The polar lipids of 16-35-5 were consisted of phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. The main fatty acids were summed feature 3 (Cω7 and/or Cω6, 25.2 %), summed feature 8 (Cω7 and/or Cω6, 12.9 %), C (35.2 %), and C cyclo (19.0 %). On the basis of the evidence presented in this study, 16-35-5 should be classified as representing a novel species of the genus , for which the name sp. nov., is proposed, with the type strain 16-35-5 (=CCTCC AB 2019325=KCTC 72915).
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http://dx.doi.org/10.1099/ijsem.0.004744DOI Listing
June 2019

Disrupted Brain Network Topology in Drug-naïve Essential Tremor Patients with and Without Depression : A Resting State Functional Magnetic Resonance Imaging Study.

Clin Neuroradiol 2021 Mar 9. Epub 2021 Mar 9.

Department of Neurology, West China Hospital, Sichuan University, Sichuan, China.

Purpose: This study was carried out to investigate brain functional connectome and its potential relationships with the disease severity and emotion function in patients with essential tremor with and without depressive symptoms by using resting-state functional magnetic resonance imaging and graph theory approaches.

Methods: In this study 33 essential tremor patients with depression, 45 essential tremor patients without depression and 79 age and gender-matched healthy controls were recruited to undergo a 3.0‑T imaging scan. The whole brain functional connectome was constructed by thresholding the partial correlation matrices of 116 brain regions, and the topologic properties were analyzed by using graph theory approaches and network-based statistic approaches. Nonparametric permutation test was also used for group comparisons of topological metrics. Correlation analyses between topographic features and the clinical characteristics were performed.

Results: The functional connectome in both essential tremor patients with and without depression showed abnormalities at the global level (decrease in clustering coefficient, global efficiency, and local efficiency but increase in characteristic path length) and at the nodal level (decrease nodal centralities in the cerebellum, motor cortex, prefrontal-limbic regions, default mode network) (p < 0.05, false discovery rate corrected). Moreover, essential tremor patients with depression showed higher node efficiency in superior frontal gyrus and posterior cingulate gyrus compared to essential tremor without depression.

Conclusion: Our results may provide insights into the underlying pathophysiology of essential tremor patients with and without depression and aid the development of some potential biomarkers of the depressive symptoms in patients with essential tremor.
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http://dx.doi.org/10.1007/s00062-021-01002-8DOI Listing
March 2021

Brain gray matter structures associated with trait impulsivity: A systematic review and voxel-based meta-analysis.

Hum Brain Mapp 2021 May 18;42(7):2214-2235. Epub 2021 Feb 18.

Huaxi MR Research Center (HMRRC), Department of Radiology, West China Hospital of Sichuan University, Chengdu, China.

Trait impulsivity is a multifaceted personality characteristic that contributes to maladaptive life outcomes. Although a growing body of neuroimaging studies have investigated the structural correlates of trait impulsivity, the findings remain highly inconsistent and heterogeneous. Herein, we performed a systematic review to depict an integrated delineation of gray matter (GM) substrates of trait impulsivity and a meta-analysis to examine concurrence across previous whole-brain voxel-based morphometry studies. The systematic review summarized the diverse findings in GM morphometry in the past literature, and the quantitative meta-analysis revealed impulsivity-related volumetric GM alterations in prefrontal, temporal, and parietal cortices. In addition, we identified the modulatory effects of age and gender in impulsivity-GM volume associations. The present study advances understanding of brain GM morphometry features underlying trait impulsivity. The findings may have practical implications in the clinical diagnosis of and intervention for impulsivity-related disorders.
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http://dx.doi.org/10.1002/hbm.25361DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8046062PMC
May 2021

Li-Rich Li [Ni Co Mn ]O for Anode-Free Lithium Metal Batteries.

Angew Chem Int Ed Engl 2021 Apr 3;60(15):8289-8296. Epub 2021 Mar 3.

Beijing Advanced Innovation Center for Materials Genome Engineering, Key Laboratory for Renewable Energy, Beijing Key Laboratory for New Energy Material and Devices, Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Science, Beijing, 100190, China.

Anode-free lithium metal batteries can maximize the energy density at the cell level. However, without the Li compensation from the anode side, it faces much more challenging to achieve a long cycling life with a competitive energy density than Li metal-based batteries. Here, we prolong the lifespan of an anode-free Li metal battery by introducing Li-rich Li [Ni Co Mn ]O into the cathode as a Li-ions extender. The Li [Ni Co Mn ]O can release a large amount of Li-ions during the first charging process to supplement the Li loss in the anode, then convert into NCM811, thus extending the lifespan of the battery without the introduction of inactive elements. By the benefit of Li-rich cathode and high reversibility of Li metal on Cu foil, the anode-free pouch cells enable to achieve 447 Wh kg energy density and 84 % capacity retention after 100 cycles in the condition of limited electrolyte addition (E/C ratio of 2 g Ah ).
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http://dx.doi.org/10.1002/anie.202017063DOI Listing
April 2021

Life time enhanced Fenton-like catalyst by dispersing iron oxides in activated carbon: Preparation and reactivation through carbothermal reaction.

J Hazard Mater 2021 03 8;406:124791. Epub 2020 Dec 8.

Department of Marine Environmental Engineering, National Kaohsiung University of Science and Technology, Kaohsiung, Taiwan.

Heterogeneous Fenton-like catalyst prepared by dispersing iron oxides in activated carbon ([email protected]) has frequently been assembled for advanced oxidation processes (AOPs). An intriguing but barely emphasized property of [email protected] is that it can be easily reactivated through a simple carbothermal reaction. Importantly, by this manner the life time of [email protected] could be effectively enhanced. We herein reported the synthesis of [email protected] hydrothermally with assistance of several commercially available surfactants and their performance in degrading real dye wastewater were evaluated. In general, as-synthesized [email protected] were noted to equip high Fe content. Deposited FeOx reduced the fraction of micropores but simultaneously introduced additional mesopores and macropores. Elevated magnetite content was observed in [email protected] equipped with high fraction of micropore and mesopore and macropore but fast dye degradation occurred at [email protected] possessing low fraction of micropore along with low mesopores and macropores. Reactivation via carbothermal reaction redistributed the deposited FeOx by increasing micropores while decreasing mesopores and macropores. Importantly, well dispersed FeOx synthesized with the assistance of surfactants exhibited high resistance to the corrosion in the degradation process. For the perspective of circular economy, deep understanding the material chemistry of [email protected] would be of particularly interest for further enhancing its life time.
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http://dx.doi.org/10.1016/j.jhazmat.2020.124791DOI Listing
March 2021

A randomized controlled trial for gualou danshen granules in the treatment of unstable angina pectoris patients with phlegm-blood stasis syndrome.

Medicine (Baltimore) 2020 Aug;99(33):e21593

Department of Cardiology, Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, China.

Introduction: Unstable angina pectoris is an acute exacerbation secondary to coronary artery occlusion. In routine clinical treatment, patients with unstable angina pectoris are prone to recurrence or aggravation of symptoms. Based on the traditional Chinese medicine (TCM) theory, phlegm, and blood stasis are one of the main pathological factors of unstable angina pectoris. The treatment of unstable angina pectoris with phlegm-blood stasis syndrome by Gualou Danshen granules (GLDS) has been the focus of many clinical trials. However, there is no evidence to prove the safety or clinical efficacy of GLDS.

Methods And Analysis: In this study, we will conduct a 4-week randomized, controlled feasibility study, with participants recruited from Guang'anmen Hospital, Chinese Academy of Traditional Chinese Medicine. Sixty subjects are to be diagnosed as having phlegm-blood stasis syndrome and randomly divided into a treatment group (GLDS) and placebo group in a 1:1 ratio. Result measurements will include therapeutic indicators (Clinical Symptom Rating Scale, Phlegm-Blood Stasis Syndrome Scale, and Seattle Angina Questionnaire) and safety indicators (blood routine, urine routine, electrocardiogram, liver function, and kidney function). The clinical data management system (http://www.tcmcec.net/) will be used to collect and manage data. Quality control will be implemented according to good clinical practice.

Discussion: Previous TCM clinical trials have investigated if adding GLDS to standard routine treatment can improve the therapeutic effect in patients with unstable angina pectoris. This study focuses on the safety and efficacy of GLDS on unstable angina pectoris of phlegm-blood stasis type, in order to obtain relevant clinical evidence.

Trial Registration: This study is approved by the Ethics Committee of Guang'anmen Hospital of the China Academy of Chinese Medical Sciences (no. 2019-187-KY-02) and is registered with chictr.org (registration number ChiCTR2000031780).
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http://dx.doi.org/10.1097/MD.0000000000021593DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7437832PMC
August 2020

sp. nov., isolated from Arctic tundra soil.

Int J Syst Evol Microbiol 2020 May;70(5):3455-3461

China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, PR China.

A Gram-stain-negative, rod-shaped, green-pigmented, aerobic and motile bacterium, strain R3-44, was isolated from Arctic tundra soil. Stain R3-44 clustered closely with members of the genus , which belongs to the family , and showed the highest 16S rRNA sequence similarity to AR2 (96.10%). Strain R3-44 grew optimally at pH 7.0, 28 °C and in the presence of 0-0.5 % (w/v) NaCl. The predominant respiratory isoprenoid quinone of strain R3-44 was identified as ubiquinone Q-8. The polar lipids consisted of phosphatidylglycerol, phosphatidylethanolamine, unidentified aminolipid and unidentified phospholipid. The main fatty acids were summed feature 3 (comprising C ω7 and/or C ω6, 40.6 %) and C (29.3 %). The DNA G+C content of strain R3-44 was 60.8 mol%. On the basis of the evidence presented in this study, strain R3-44 represents a novel species of the genus , for which the name sp. nov. is proposed, with the type strain R3-44 (=CCTCC AB 2010422=KCTC 72602).
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http://dx.doi.org/10.1099/ijsem.0.004194DOI Listing
May 2020

Shared gray matter alterations in individuals with diverse behavioral addictions: A voxel-wise meta-analysis.

J Behav Addict 2020 Apr 1;9(1):44-57. Epub 2020 Apr 1.

1 Huaxi MR Research Center (HMRRC), Department of Radiology, West China Hospital of Sichuan University, Chengdu, Sichuan,China.

Background And Aims: Numerous studies on behavioral addictions (BAs) have reported gray matter (GM) alterations in multiple brain regions by using voxel-based morphometry (VBM). However, findings are poorly replicated and it remains elusive whether distinct addictive behaviors are underpinned by shared abnormalities. In this meta-analysis, we integrated VBM studies on different BAs to investigate common GM abnormalities in individuals with BAs.

Methods: Numerous studies on behavioral addictions (BAs) have reported gray matter (GM) alterations in multiple brain regions by using voxel-based morphometry (VBM). However, findings are poorly replicated and it remains elusive whether distinct addictive behaviors are underpinned by shared abnormalities. In this meta-analysis, we integrated VBM studies on different BAs to investigate common GM abnormalities in individuals with BAs.

Results: Twenty studies including 505 individuals with BAs and 564 healthy controls met the inclusion criteria. Compared with healthy controls, individuals with BAs showed GM atrophy in the left anterior cingulate (extending to the left medial superior frontal gyrus and bilateral orbitofrontal gyrus), right putamen and right supplementary motor area. Subgroup analysis found heterogeneity in gender and subtypes of BAs. Meta-regression revealed that GM decreases in the left anterior cingulate and right supplementary motor area were positively correlated with addictive severity. Higher impulsivity was associated with smaller volume of the left anterior cingulate.

Discussion And Conclusions: Our findings on BAs were mainly derived from internet gaming disorder (IGD) and pathological gambling (PG) studies, preliminarily suggesting that GM atrophy in the prefrontal and striatal areas might be a common structural biomarker of BAs.
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http://dx.doi.org/10.1556/2006.2020.00006DOI Listing
April 2020

IFITM3 affects the level of antibody response after influenza vaccination.

Emerg Microbes Infect 2020 Dec;9(1):976-987

National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Prevention and Control, Beijing, People's Republic of China.

Interferon-induced transmembrane protein 3 (IFITM3) as an antiviral factor can inhibit replication of several viruses including influenza virus. A single-nucleotide polymorphism rs12252-C of results in a truncated IFITM3 protein lacking its first 21 amino acids, which is much higher in the Han Chinese population and associated with severe illness in adults infected with pandemic influenza H1N1/09 virus. To investigate if IFITM3 or rs12252-C could affect the antibody response after influenza vaccination, we detected the haemagglutination inhibition (HI) of 171 healthy young adult volunteers ( rs12252-C/C, C/T, T/T carriers) and in an IFITM3-deletion mouse model () after trivalent inactivated vaccine (TIV) immunization. Seroconversion rates for H1N1, H3N2 and B viruses in rs12252-C/C genotype carriers was lower compared with C/T and T/T donors Significantly lower levels of specific antibodies to H1N1, H3N2 and B viruses and total IgG were observed in mice. Correspondingly, the numbers of splenic germinal centre (GC) B cells, plasma cells, TIV-specific IgG antibody secreting cells and T follicular helper cells in mice were lower compared with wild type mice. However, the number of memory B cells was higher in mice at day 7 after booster. The HI level of mice remained lower than WT mice after third vaccination. Moreover, the transcriptional network regulating GC B cell and plasma cell differentiation was abnormal in mice. Our results indicate that IFITM3 deletion attenuated the antibody response. The mechanism of influenza-IFITM3 interactions affecting the antibody response requires further investigation.
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http://dx.doi.org/10.1080/22221751.2020.1756696DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7269036PMC
December 2020

A Multiple-Instance Densely-Connected ConvNet for Aerial Scene Classification.

IEEE Trans Image Process 2020 Mar 3. Epub 2020 Mar 3.

In contrast with nature scenes, aerial scenes are often composed of many objects crowdedly distributed on the surface in bird's view, the description of which usually demands more discriminative features as well as local semantics. However, when applied to scene classification, most of the existing convolution neural networks (ConvNets) tend to depict global semantics of images, and the loss of low- and mid-level features can hardly be avoided, especially when the model goes deeper. To tackle these challenges, in this paper, we propose a multiple-instance densely-connected ConvNet (MIDC-Net) for aerial scene classification. It regards aerial scene classification as a multiple-instance learning problem so that local semantics can be further investigated. Our classification model consists of an instance-level classifier, a multiple instance pooling and followed by a bag-level classification layer. In the instance-level classifier, we propose a simplified dense connection structure to effectively preserve features from different levels. The extracted convolution features are further converted into instance feature vectors. Then, we propose a trainable attention-based multiple instance pooling. It highlights the local semantics relevant to the scene label and outputs the bag-level probability directly. Finally, with our bag-level classification layer, this multiple instance learning framework is under the direct supervision of bag labels. Experiments on three widely-utilized aerial scene benchmarks demonstrate that our proposed method outperforms many state-of-the-art methods by a large margin with much fewer parameters.
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http://dx.doi.org/10.1109/TIP.2020.2975718DOI Listing
March 2020

sp. nov., a bacterium isolated from Arctic marine sediment.

Int J Syst Evol Microbiol 2020 Mar;70(3):2072-2078

China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, PR China.

An aerobic, Gram-stain-negative, motile, rod or long-rod-shaped bacterial isolate, strain MK6-18, was isolated from a marine sediment sample from Kongsfjorden, Arctic. The bacterium grew optimally at 20 °C, pH 7.0 and in the presence of 1.0-2.0 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain MK6-18 belonged to the genus . Its closest phylogenetic neighbour was NH52J showing 96.97 % 16S rRNA gene sequence similarity. The genome of strain MK6-18 is 4.2 Mb long in size with a G+C content of 59.5 mol%. The average nucleotide identity value between the genomes of strain MK6-18 and NH52J, was 78.0 %. Similar to other species of the genus , strain MK6-18 had ubiquinone 10 as the predominant ubiquinone and C, C, summed feature 3 (Cω7cω6) and summed feature 8 (Cω7cω6) as the major fatty acids. The polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine; one unidentified polar lipid, one unidentified aminolipid and one unidentified lipid were also detected. This is the first time that a member of the genus has been isolated from the Arctic, which may promote the study of the distribution characteristics and environmental adaptability of this genus. On the basis of the data provided here, strain MK6-18 should be classed as representing a novel species of the genus , for which the name sp. nov. is proposed. The type strain is MK6-18 (=CCTCC AB 2018219=KCTC 72187).
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http://dx.doi.org/10.1099/ijsem.0.004018DOI Listing
March 2020

Correction to: Hymenobacter oligotrophus sp. nov., isolated from a contaminated agar plate.

Antonie Van Leeuwenhoek 2020 06;113(6):861

China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan, 430072, China.

In the original publication, the deposit number of strain sh-6 was incorrectly published as "CCTCC AB 2016064" throughout the article.
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http://dx.doi.org/10.1007/s10482-020-01389-3DOI Listing
June 2020

Human-infecting influenza A (H9N2) virus: A forgotten potential pandemic strain?

Authors:
Wenjun Song Kun Qin

Zoonoses Public Health 2020 05 12;67(3):203-212. Epub 2020 Jan 12.

National Institute of Viral Disease Control and Prevention, China CDC, Beijing, China.

Continuously emergence of human infection with avian influenza A virus poses persistent threat to human health, as illustrated in H5N1, H7N9 and recent surge of H9N2 infections. Long-term prevalence of H9N2 avian influenza A virus in China and adjacent regions favours the interspecies transmissions from avian to human. Establishment of multiple genotypes of H9N2 variants in this region contributes to the emergence of novel H7N9 and H10N8 viruses which caused human fatalities. Recent increasing human infection with H9N2 virus in China highlights the necessity to closely monitor the interspecies transmission events. Available human H9N2 sequences revealed that Y280/G9 lineage was responsible for the most of human cases. Presence of adaptive mutations beyond the human-like receptor binding was indicative of the capacity of readily infecting new hosts without prior adaptation. Moreover, enlarged host range of H9N2 virus in this region substantially increased the transmission among mammals. Meanwhile, serological surveys implied human was more susceptible to H9N2 infection, compared with panzootic H5 and H7 subtype avian influenza virus. Thus, control at the source will be the ultimate and effective option for H9N2 pandemic preparedness. This review comprehensively summarized recent updates on H9N2 human infections, aiming to shed light on the prevention strategies against this strain with pandemic potential.
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http://dx.doi.org/10.1111/zph.12685DOI Listing
May 2020

miR-647 inhibits glioma cell proliferation, colony formation and invasion by regulating HOXA9.

J Gene Med 2020 03 20;22(3):e3153. Epub 2020 Jan 20.

Department of Neurosurgery, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong, China.

Background: MicroRNA-647 (miR-647) has been reported to regulate tumor development, although its role in glioma remains unclear.

Methods: miR-647 expression in glioma cells and normal cells was measured using a quantitative real-time polymerase chain reaction. The effects of miR-647 expression on glioma cell proliferation, cell apoptosis, colony formation and cell invasion were measured using a cell counting kit-8 assay, flow cytometry, a colony formation assay and a transwell invasion assay. Luciferase activity reporter and western blot assays were conducted to explore whether homeobox A9 (HOXA9) was a direct target of miR-647.

Results: We found that miR-647 expression was downregulated in glioma cell lines compared to the normal cell line. Overexpression of miR-647 inhibits glioma cell proliferation, colony formation and cell invasion, although it promotes apoptosis in vitro. HOXA9 was validated a direct target of miR-647 and the overexpression of HOXA9 reversed the effects of miR-647 on glioma cell behavior.

Conclusions: The identification of the miR-647/HOXA9 axis will advance our understanding underlying glioma progression and provide novel therapeutic targets for glioma treatment.
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http://dx.doi.org/10.1002/jgm.3153DOI Listing
March 2020

The BRCC3 regulated by Cdk5 promotes the activation of neuronal NLRP3 inflammasome in Parkinson's disease models.

Biochem Biophys Res Commun 2020 02 29;522(3):647-654. Epub 2019 Nov 29.

Department of Human Anatomy, Guilin Medical University, Guilin, 541100, Guangxi, China; Guangxi Key Laboratory of Brain and Cognitive Neuroscience, Guilin Medical University, Guilin, 541100, Guangxi, China. Electronic address:

BRCA1-BRCA2-containing complex subunit 3 (BRCC3), a Lys-63-specific deubiquitinase, is a member of the JAMM/MPN family of zinc metalloproteases. BRCC3 have been shown to promote the inflammasome activation by deubiquitinating NOD-like receptor containing pyrin domain 3 (NLRP3). We reported the involvement of neuronal inflammasome in Parkinson's Disease (PD), but the molecular mechanism remains unknown. In this study, we showed that BRCC3 expression was increased in PD models. Knock-down of BRCC3 with shRNA lentivirus decreased NLRP3 neuronal inflammasome. Interestingly, upregulating cyclin-dependent kinase 5 (Cdk5) increased the expression of BRCC3 in HEK293 cell, while inhibition of Cdk5 decreased the upregulated BRCC3 level in MPP-induced PD cell model. The interaction between Cdk5 and BRCC3 was further confirmed by immunoprecipitation. Moreover, inhibition of Cdk5 suppressed the expression of NLRP3, pro-caspase-1, the adaptor molecule apoptosis-associated speck-like protein containing a CARD (ASC) and interleukin-1 beta (IL-1β). Besides, inhibition of BRCC3 blocked the increased secretion of IL-1β. Together, these results suggest that Cdk5-mediated BRCC3 expression may play a critical role in neuronal inflammation by regulating the NLRP3 inflammasome in PD.
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http://dx.doi.org/10.1016/j.bbrc.2019.11.141DOI Listing
February 2020
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