Publications by authors named "Kun Qiao"

79 Publications

Structural insights into the inhibition of bacterial RecA by naphthalene polysulfonated compounds.

iScience 2021 Jan 17;24(1):101952. Epub 2020 Dec 17.

Department of Pharmacology, Shantou University Medical College, Shantou 515041, China.

As a promising target for alternative antimicrobials, bacterial recombinase A (RecA) protein has attracted much attention for its roles in antibiotic-driven SOS response and mutagenesis. Naphthalene polysulfonated compounds (NPS) such as suramin have previously been explored as antibiotic adjuvants targeting RecA, although the underlying structural bases for RecA-ligand interactions remain obscure. Based on our predictions and documented activity of NPS , we conclude that the analyzed NPS likely interact with Tyr103 (Y103) and other key residues in the ATPase activity center (pocket A). For validation, we generated recombinant RecA proteins (wild-type versus Y103 mutant) to determine the binding affinities for RecA protein interactions with suramin and underexamined NPS in isothermal titration calorimetry. The corresponding dissociation constants () ranged from 11.5 to 18.8 μM, and Y103 was experimentally shown to be critical to RecA-NPS interactions.
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http://dx.doi.org/10.1016/j.isci.2020.101952DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7797525PMC
January 2021

Bacterial seed endophyte shapes disease resistance in rice.

Nat Plants 2021 Jan 4;7(1):60-72. Epub 2021 Jan 4.

Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Ministry of Agriculture, Institute of Pesticide and Environmental Toxicology, Zhejiang University, Hangzhou, China.

Cereal crop production is severely affected by seed-borne bacterial diseases across the world. Locally occurring disease resistance in various crops remains elusive. Here, we have observed that rice plants of the same cultivar can be differentiated into disease-resistant and susceptible phenotypes under the same pathogen pressure. Following the identification of a seed-endophytic bacterium as the resistance-conferring agent, integration of high-throughput data, gene mutagenesis and molecular interaction assays facilitated the discovery of the underlying mode of action. Sphingomonas melonis that is accumulated and transmitted across generations in disease-resistant rice seeds confers resistance to disease-susceptible phenotypes by producing anthranilic acid. Without affecting cell growth, anthranilic acid interferes with the sigma factor RpoS of the seed-borne pathogen Burkholderia plantarii, probably leading to impairment of upstream cascades that are required for virulence factor biosynthesis. The overall findings highlight the hidden role of seed endophytes in the phytopathology paradigm of 'disease triangles', which encompass the plant, pathogens and environmental conditions. These insights are potentially exploitable for modern crop cultivation threatened by globally widespread bacterial diseases.
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http://dx.doi.org/10.1038/s41477-020-00826-5DOI Listing
January 2021

Crosstalk of cholinergic pathway on thyroid disrupting effects of the insecticide chlorpyrifos in zebrafish (Danio rerio).

Sci Total Environ 2021 Feb 14;757:143769. Epub 2020 Nov 14.

Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insect Pests, Institute of Pesticide and Environmental Toxicology, Zhejiang University, Hangzhou 310058, PR China.

Chlorpyrifos is a widely used organophosphate insecticide and ubiquitously detected in the environment. However, little attention has been paid to its endocrine disrupting effect to non-target organisms. In the present study, zebrafish was exposed to 13 and 65 μg/L of chlorpyrifos for 7 and 10 days to determine the induced neurotoxicity and the alteration of thyroid metabolism. The 120 h LC and LC of chlorpyrifos was estimated as 1.35 mg/L and 0.62 mg/L based on the acute embryo toxicity assay, respectively. The acetylcholinesterase (AChE) inhibitory was detected by 13 μg/L chlorpyrifos and could be reversed by the co-exposure of 100 and 1000 μg/L anticholinergic agent atropine. For thyroid hormone level, 13 and 65 μg/L of chlorpyrifos induced increased free T levels in 10 dpf (days post-fertilization). The expression of thyroid related genes in 7 and 10 dpf exposed zebrafish were measured by the quantitative Real-Time PCR (qRT-PCR) assay. The mRNA expression of tshba, thrb, crhb, ttr, tpo, ugt1ab and slc5a5 had significant change. However, the alterations of thyroid hormone and mRNA expression could be partly rescued by the addition of atropine. The molecular docking of chlorpyrifos and T to the thyroid receptor β in zebrafish using homology modelling and CDOCKER procedures shown weaker binding ability of chlorpyrifos compared to T. Therefore, we concluded that the disturbance of thyroid signaling in zebrafish might arise from the developmental neurotoxicity induced by chlorpyrifos.
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http://dx.doi.org/10.1016/j.scitotenv.2020.143769DOI Listing
February 2021

Sexual Differences in Physiological and Transcriptional Responses to Salinity Stress of .

Front Plant Sci 2020 19;11:517962. Epub 2020 Oct 19.

The State Key Laboratory of Subtropical Silviculture, Zhejiang Agriculture and Forestry University, Hangzhou, China.

Willow (), a dioecious plant, is an important ornamental tree species in the world. , a perennial woody plant species naturally distributed on the Songnen Plain saline-alkali land in northeast China, has a high saline condition. To study the sexual differences of in salinity tolerance, the physiological and transcriptional responses to salinity were compared between female and male cuttings. Under salinity stress, the female leaves exhibited higher superoxide dismutase and peroxidase activities and photosynthetic capacity, and lower HO contents than those of male leaves. Under salinity stress, sodium (Na) accumulation in female leaves was lower than that in the male leaves. The non-invasive micro-test showed that the net Na efflux in the salt-treated female roots was higher than that in male roots. Physiological responses revealed that female cuttings were more tolerant than males, which may be mainly due to females having lower leaf Na accumulation and higher root Na efflux capacity than males. Transcriptional analyses showed that 108 differentially expressed salt-responsive genes were identified in both female and male roots; most of these showed sexual differences in expression patterns under salinity stress. RNA-seq combined with qPCR analysis showed that the salt-induced expression of four Na/H antiporter () genes () in female roots was higher than that in male roots. Transcriptional analyses revealed that the higher Na efflux capacity in female roots than in male roots may be closely related to the differential expression of salt-responsive genes, especially genes.
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http://dx.doi.org/10.3389/fpls.2020.517962DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7604296PMC
October 2020

Plant protein modified natural cellulose with multiple adsorption effects used for bilirubin removal.

Int J Biol Macromol 2021 Jan 22;166:179-189. Epub 2020 Oct 22.

Key Laboratory of Wound Repair and Regeneration of PLA, Chinese PLA General Hospital, Medical College of PLA, Beijing 100853, PR China. Electronic address:

In this study, bacterial cellulose (BC)/soy protein isolate (SPI) composite membranes were prepared by in situ cross-linked polymerization, and used as efficient blood compatible adsorbents to remove bilirubin. The obtained composite membranes were successively characterized by FTIR, SEM, AFM, contact angle test and hemolysis assay, which exhibited unique protein coated 3D fibrous network structures, hydrophobic surfaces and outstanding blood compatibility due to the incorporation of SPI. The BC/SPI membranes with high SPI content showed high adsorption efficiency, short adsorption equilibrium time (2 h) and multiple adsorption effects on bilirubin. The adsorption rate for free bilirubin of BC/SPI5 membrane could reach 78.8% when the bilirubin concentration was 100 mg L, while it increased to over 96.5% when the initial bilirubin concentration exceeded 400 mg L. More importantly, the BC/SPI5 membrane still exhibited high adsorption rate (over 70%) in presence of albumin. Furthermore, the composite membrane could also maintain high dynamic adsorption efficiency in self-made hemoperfusion devices. This novel naturally-derived membrane is an economical and efficient absorbent for the remove of bilirubin, and will provide new ideas for therapy of hemoperfusion without plasma separation process.
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http://dx.doi.org/10.1016/j.ijbiomac.2020.10.131DOI Listing
January 2021

Morphological and physiological responses of two willow species from different habitats to salt stress.

Sci Rep 2020 10 26;10(1):18228. Epub 2020 Oct 26.

College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin, 150030, China.

Plant salt tolerance is a complex mechanism, and different plant species have different strategies for surviving salt stress. In the present study, we analyzed and compared the morphological and physiological responses of two willow species (Salix linearistipularis and Salix matsudana) from different habitats to salt stress. S. linearistipularis exhibited higher seed germination rates and seedling root Na efflux than S. matsudana under salt stress. After salt treatment, S. linearistipularis leaves exhibited less Na accumulation, loss of water and chlorophyll, reduction in photosynthetic capacity, and damage to leaf cell structure than leaves of S. matsudana. Scanning electron microscopy combined with gas chromatography mass spectrometry showed that S. linearistipularis leaves had higher cuticular wax loads than S. matsudana leaves. Overall, our results showed that S. linearistipularis had higher salt tolerance than S. matsudana, which was associated with different morphological and physiological responses to salt stress. Furthermore, our study suggested that S. linearistipularis could be a promising tree species for saline-alkali land greening and improvement.
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http://dx.doi.org/10.1038/s41598-020-75349-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7588438PMC
October 2020

Identification of long-term survival-associated gene in breast cancer.

Aging (Albany NY) 2020 10 20;12(20):20332-20349. Epub 2020 Oct 20.

Harbin Medical University, Harbin 150081, China.

Breast cancer patients at the same stage may show different clinical prognoses or different therapeutic effects of systemic therapy. Differentially expressed genes of breast cancer were identified from GSE42568. Through survival, receiver operating characteristic (ROC) curve, random forest, GSVA and a Cox regression model analyses, genes were identified that could be associated with survival time in breast cancer. The molecular mechanism was identified by enrichment, GSEA, methylation and SNV analyses. Then, the expression of a key gene was verified by the TCGA dataset and RT-qPCR, Western blot, and immunohistochemistry. We identified 784 genes related to the 5-year overall survival time of breast cancer. Through ROC curve and random forest analysis, 10 prognostic genes were screened. These were integrated into a complex by GSVA, and high expression of the complex significantly promoted the recurrence-free survival of patients. In addition, key genes were related to immune and metabolic-related functions. Importantly, we identified methylation of MEX3A and TBC1D 9 and mutations events. Finally, the expression of UGCG was verified by the TCGA dataset and by experimental methods in our own samples. These results indicate that 10 genes may be potential biomarkers and therapeutic targets for long-term survival in breast cancer, especially UGCG.
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http://dx.doi.org/10.18632/aging.103807DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7655188PMC
October 2020

Regulatory science for hernia mesh: Current status and future perspectives.

Bioact Mater 2021 Feb 8;6(2):420-432. Epub 2020 Sep 8.

School of Material Science and Engineering, University of Science and Technology Beijing, 30 Xueyuan Road, Haidian District, Beijing, China.

Regulatory science for medical devices aims to develop new tools, standards and approaches to assess the safety, effectiveness, quality and performance of medical devices. In the field of biomaterials, hernia mesh is a class of implants that have been successfully translated to clinical applications. With a focus on hernia mesh and its regulatory science system, this paper collected and reviewed information on hernia mesh products and biomaterials in both Chinese and American markets. The current development of regulatory science for hernia mesh, including its regulations, standards, guidance documents and classification, and the scientific evaluation of its safety and effectiveness was first reported. Then the research prospect of regulatory science for hernia mesh was discussed. New methods for the preclinical animal study and new tools for the evaluation of the safety and effectiveness of hernia mesh, such as computational modeling, big data platform and evidence-based research, were assessed. By taking the regulatory science of hernia mesh as a case study, this review provided a research basis for developing a regulatory science system of implantable medical devices, furthering the systematic evaluation of the safety and effectiveness of medical devices for better regulatory decision-making. This was the first article reviewing the regulatory science of hernia mesh and biomaterial-based implants. It also proposed and explained the concepts of evidence-based regulatory science and technical review for the first time.
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http://dx.doi.org/10.1016/j.bioactmat.2020.08.021DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7490592PMC
February 2021

Electrochemical Insights, Developing Strategies, and Perspectives toward Advanced Potassium-Sulfur Batteries.

Small 2020 Oct 22;16(42):e2003386. Epub 2020 Sep 22.

Key Laboratory for Liquid-Solid Structural Evolution and Processing of Materials (Ministry of Education), School of Materials Science and Engineering, Shandong University, Jinan, 250061, China.

The boosting demand for high-capacity energy storage systems requires innovative battery technologies with low-cost and sustainability. The advancement of potassium-sulfur (K-S) batteries have been triggered recently due to abundant resource and cost effectiveness. However, the functional performance of K-S batteries is fundamentally restricted by the vague understanding of K-S electrochemistry and the imperfect cell components or architectures, facing the issues of low cathode conductivity, intermediate shuttle loss, poor anode stability, electrode volume fluctuation, etc. Inspired by considerable research efforts on rechargeable metal-sulfur batteries, the holistic K-S system can be stabilized and promoted through various strategies on rational physical regulation and chemical engineering. In this review, first an attempt is made to address the electrochemical kinetic concept of K-S system on the basis of the emerging studies. Then, the classification of performance-improving strategies is thoroughly discussed in terms of specific battery component and prospective outlooks in materials optimization, structure innovations, as well as relevant electrochemistry are provided. Finally, the critical perspectives and challenges are discussed to demonstrate the forward-looking developmental directions of K-S batteries. This review not only endeavors to provide a deep understanding of the electrochemistry mechanism and rational designs for high-energy K-S batteries, but also encourages more efforts in their large-scale practical realization.
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http://dx.doi.org/10.1002/smll.202003386DOI Listing
October 2020

Reversing cold tumors to hot: An immunoadjuvant-functionalized metal-organic framework for multimodal imaging-guided synergistic photo-immunotherapy.

Bioact Mater 2021 Feb 28;6(2):312-325. Epub 2020 Aug 28.

Molecular Diagnosis and Treatment Center for Infectious Diseases, Dermatology Hospital, Southern Medical University, Guangzhou, 510091, China.

Immunotherapy assays using immunoadjuvants and tumor antigens could greatly increase the survival rates of patients with malignant tumors. As effective carriers, metal-organic frameworks (MOFs) have been widely utilized in cancer therapy due to their remarkable histocompatibility and low toxicity. Herein, we constructed a multimodal imaging-guided synergistic cancer photoimmunotherapy by employing a specific MOF (MIL101-NH) as the core carrier; the MOF was dual-dressed with photoacoustic and fluorescent signal donors (indocyanine green, ICG) and immune adjuvants (cytosine-phosphate-guanine sequence, CpG) and named ICG-CpG@MOF. This nanocarrier could passively target the tumor site through the EPR effect and achieve multimodal imaging (fluorescence, photoacoustic, photothermal and magnetic resonance imaging) of the tumor. Synergistic cancer photoimmunotherapy was achieved via simultaneous photodynamic and photothermal methods with 808 nm laser irradiation. ICG-CpG@MOF achieved the GSH-controlled release of immunoadjuvant into the tumor microenvironment. Furthermore, the released tumor-associated antigen along with CpG could induce the transformation of tumor cells from cold to hot by activating the immune system, which significantly enhanced tumor cytotoxicity and achieved high cure rates with minimal side-effects. This strategy utilizing multimodal imaging and synergistic cancer photoimmunotherapy provides a promising approach for the diagnosis and treatment of cancer.
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http://dx.doi.org/10.1016/j.bioactmat.2020.08.005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7475520PMC
February 2021

Relationships among smoking abstinence self-efficacy, trait coping style and nicotine dependence of smokers in Beijing.

Tob Induc Dis 2020 1;18:72. Epub 2020 Sep 1.

School of Public Health, Capital Medical University, Beijing, China.

Introduction: Psychological, physiological and social factors play an important role in the initiation, persistence, dependence and relapse of smoking behaviors, and coping style and smoking abstinence self-efficacy can all affect nicotine dependence.

Methods: A cross-sectional sample of 568 quitters from 19 communities in Beijing in 2019 was surveyed. Demographic information and psychological characteristics of smokers were collected by an interview questionnaire, and psychological traits scales including the Smoking Abstinence Self-Efficacy (SASE) and the Trait Coping Style Questionnaire (TCSQ). We compared differences in psychological traits across demographic information and explored the relationship between nicotine dependence and coping styles and self-efficacy in refusing to smoke.

Results: Significant differences were identified in self-efficacy in refusing to smoke and across dimensions among quitters by gender, job type, education level, and monthly income level (all p<0.05). Males had lower self-efficacy in the habitual/addictive context than females; retirees had better overall self-efficacy and self-efficacy in the negative/emotional context than business service workers and professionals; and high-educated, high-income quitters had lower self-efficacy in the negative/emotional context. There are significant differences in positive coping styles among quitters of different ages, levels of education, and types of work (all p<0.05). The results further showed that the underage population, highly educated population, and practitioners other than those in retirement, are less likely to use positive coping styles. Interventional effects analysis showed that a higher sense of self-efficacy in addictive contexts can counteract some of the negative coping styles that induce smoking.

Conclusions: Self-efficacy played an indirect mediating role between negative coping style and nicotine dependence; individuals who used more negative coping styles were more likely to engage in smoking and therefore were more nicotine dependent. Hence, it is necessary to reduce the use of negative coping strategies and improve the self-efficacy of smoking abstinence in the face of addiction.
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http://dx.doi.org/10.18332/tid/125401DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7485440PMC
September 2020

Perception of Patient Safety and the Reporting System Between Medical Staffs and Patients in China: A Cross-Sectional Online Study.

J Patient Saf 2020 Sep 8. Epub 2020 Sep 8.

School of Public Health, Capital Medical University.

Objective: This study aimed to understand the perception of patient safety and the reporting system in public in China, and make further recommendations for the optimization of the reporting system of patient safety.

Methods: The following data were collected through an online questionnaire from medical staffs and patients: recognition of patient safety, comments on patient participation, comments on spontaneous reports, attitudes toward the principles of spontaneous reports, and willingness to participate. This information was presented with frequency and percentage with 95% confidence intervals (CIs). Spearman rank correlation was used to evaluate the association of those data.

Results: A total of 27,493 valid questionnaires were collected in this study. The participants who knew patient safety very well, regarded patients as an essential part to enhance patient safety, viewed spontaneous reports helpful, agreed on the 3 principles of voluntariness, anonymity, and nonpunishment of the reporter, and were willing to participate in reporting were accounted for 39.2% (95% CI, 38.6%-39.8%), 31.2% (95% CI, 30.7%-31.8%), 25.2% (95% CI, 24.7%-25.7%), 24.0% (95% CI, 23.5%-24.5%), and 19.9% (95% CI, 19.4%-20.4%), respectively. A moderate positive correlation was observed between comments on spontaneous reports and attitudes toward the principles of spontaneous reports (medical staffs versus patients, rs = 0.452 versus rs = 0.439; both, P < 0.01), as well as comments on patient participation and comments on spontaneous reports (medical staffs versus patients, rs = 0.410 versus rs = 0.460; both, P < 0.01).

Conclusions: The awareness of patient safety may influence the choices of the other questions subsequently and may affect their voluntariness for safety reports ultimately. Pertinent measures, such as publicity of the relevant concept, simplification of the reporting procedures, and promotion of incentive mechanisms, should be adopted to promote the optimization of the reporting system.
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http://dx.doi.org/10.1097/PTS.0000000000000773DOI Listing
September 2020

Comparison of cadmium uptake and transcriptional responses in roots reveal key transcripts from high and low-cadmium tolerance ryegrass cultivars.

Ecotoxicol Environ Saf 2020 Oct 19;203:110961. Epub 2020 Jul 19.

College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin, 150030, PR China. Electronic address:

Cadmium (Cd), which seriously affects plant growth and crop production, is harmful to humans. Previous studies revealed ryegrass (Lolium multiflorum Lam.) exhibits Cd tolerance, and may be useful as a potential hyperaccumulator because of its wide distribution. In this study, the physiological and transcriptional responses of two ryegrass cultivars [i.e., high (LmHC) and low (LmLC) Cd tolerance] to Cd stress were investigated and compared. The Cd tolerance of LmHC was greater than that of LmLC at various Cd concentrations. The uptake of Evans blue dye revealed that Cd-induced root cell mortality was higher in LmLC than in LmHC after a 12-h Cd treatment. Furthermore, the content and influx rate of Cd in LmLC roots were greater than in LmHC roots under Cd stress conditions. The RNA sequencing and quantitative real-time PCR data indicated that the Cd transport regulatory genes (ABCG37, ABCB4, NRAMP4, and HMA5) were differentially expressed between the LmLC and LmHC roots. This expression-level diversity may contribute to the differences in the Cd accumulation and translocation between LmLC and LmHC. These findings may help clarify the physiological and molecular mechanisms underlying ryegrass responses to Cd toxicity. Additionally, ryegrass may be able to hyperaccumulate toxic heavy metals during the phytoremediation of contaminated soil.
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http://dx.doi.org/10.1016/j.ecoenv.2020.110961DOI Listing
October 2020

Correction to: LINC00673 is activated by YY1 and promotes the proliferation of breast cancer cells via the miR-515-5p/MARK4/Hippo signaling pathway.

J Exp Clin Cancer Res 2020 08 11;39(1):154. Epub 2020 Aug 11.

Department of Breast Surgery, Harbin Medical University Cancer Hospital, 150 Haping Road, Harbin, 150086, China.

An amendment to this paper has been published and can be accessed via the original article.
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http://dx.doi.org/10.1186/s13046-020-01645-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7418315PMC
August 2020

Molecular characterization, purification, and antioxidant activity of recombinant superoxide dismutase from the Pacific abalone Haliotis discus hannai Ino.

World J Microbiol Biotechnol 2020 Jul 14;36(8):115. Epub 2020 Jul 14.

Fisheries Research Institute of Fujian, Key Laboratory of Cultivation and High-Value Utilization of Marine Organisms in Fujian Province, Xiamen, 361013, China.

Superoxide dismutase (SOD) is an acidic metalloenzyme that scavenges free radicals produced by endogenous and exogenous substances. In the present study, the tissue distribution of the superoxide dismutase HdhCu/Zn-SOD was investigated in Haliotis discus hannai Ino. The expression profile after lipopolysaccharide (LPS) challenge was determined using quantitative real-time polymerase chain reaction (qPCR). To study the antioxidant activity of a recombinant HdhCu/Zn-SOD protein, the HdhCu/Zn-SOD gene was cloned into the pPIC9K vector and transformed into the Pichia pastoris GS115 strain by electroporation. After induction by methanol, the recombinant product was purified using immobilized metal affinity chromatography and confirmed using mass spectrometry. The optimal expression conditions were determined to be incubation with 0.5% methanol at pH 6.0, resulting in a stable expressed product with the molecular weight of approximately 17 kDa and 21 kDa. The enzymatic activity of HdhCu/Zn-SOD consistently increased with increasing Cu concentrations and showed good thermal stability. Recombinant HdhCu/Zn-SOD showed a strong ability to scavenge superoxide anions and hydroxyl radicals and protected L929 cells against the toxicity caused by HO through its in vitro antioxidant activity. The heterologous expression of HdhCu/Zn-SOD in P. pastoris and the antioxidant activity of this enzyme are reported for the first time.
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http://dx.doi.org/10.1007/s11274-020-02892-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7359182PMC
July 2020

V-ATPase d2 Subunit Plays a Role in Plant Responses to Oxidative Stress.

Genes (Basel) 2020 06 25;11(6). Epub 2020 Jun 25.

Key Laboratory of Saline-Alkali Vegetation Ecology Restoration, Northeast Forestry University, Ministry of Education, Harbin 150040, China.

Vacuolar-type H-ATPase (V-ATPase), a multisubunit proton pump located on the endomembrane, plays an important role in plant growth. The V-ATPase d subunit (VHA-d) consists of two isoforms; AtVHA-d1 and AtVHA-d2. In this study, the function of was investigated. Histochemical analysis revealed that the expression of and was generally highly overlapping in multiple tissues at different developmental stages of Arabidopsis. Subcellular localization revealed that AtVHA-d2 was mainly localized to the vacuole. expression was significantly induced by oxidative stress. Analysis of phenotypic and HO content showed that the mutant was sensitive to oxidative stress. The noninvasive microtest monitoring demonstrated that the net H influx in the roots was weaker than that in the wild-type under normal conditions. However, oxidative stress resulted in the H efflux in roots, which was significantly different from that in the wild-type. RNA-seq combined with qPCR analysis showed that the expression of several members of the plasma membrane H-ATPase gene () family in was significantly different from that in the wild-type. Overall, our results indicate that AtVHA-d2 plays a role in Arabidopsis in response to oxidative stress by affecting H flux and gene expression.
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http://dx.doi.org/10.3390/genes11060701DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7349310PMC
June 2020

Heterologous Expression of Nitrate Assimilation Related-Protein DsNAR2.1/NRT3.1 Affects Uptake of Nitrate and Ammonium in Nitrogen-Starved .

Int J Mol Sci 2020 Jun 4;21(11). Epub 2020 Jun 4.

College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, China.

Nitrogen (N) is an essential macronutrient for plant growth. Plants absorb and utilize N mainly in the form of nitrate (NO) or ammonium (NH). In this study, the nitrate transporter DsNRT3.1 (also known as the nitrate assimilation-related protein DsNAR2.1) was characterized from . A quantitative PCR (qPCR) analysis showed that the expression was induced by NO. Under N-starvation conditions, the transformed seedlings expressing had longer roots and a greater fresh weight than the wild type. Subcellular localization showed that DsNRT3.1 was mainly localized to the plasma membrane in root hair cells. Non-invasive micro-test (NMT) monitoring showed that the root hairs of N-starved transformed seedlings had a stronger NO and NH influx than the wild-type seedlings, using with NO or NH as the sole N source; contrastingly, transformed seedlings only had a stronger NO influx when NO and NH were present simultaneously. In addition, the qPCR analysis showed that the expression of genes (), and particularly of , in the transformed differed from that in the wild type. Overall, our results suggest that the heterologous expression of affects seedlings' growth by enhancing the NO and NH uptake in N-starved . This may be related to the differential expression of genes.
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http://dx.doi.org/10.3390/ijms21114027DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7312895PMC
June 2020

QSAR models for the acute toxicity of 1,2,4-triazole fungicides to zebrafish (Danio rerio) embryos.

Environ Pollut 2020 Oct 17;265(Pt B):114837. Epub 2020 May 17.

Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insect Pests, Institute of Pesticide and Environmental Toxicology, Zhejiang University, Hangzhou, 310058, PR China. Electronic address:

In recent decades, the 1,2,4-triazole fungicides are widely used for crop diseases control, and their toxicity to wild lives and pollution to ecosystem have attracted more and more attention. However, how to quickly and efficiently evaluate the toxicity of these compounds to environmental organisms is still a challenge. In silico method, such like Quantitative Structure-Activity Relationship (QSAR), provides a good alternative to evaluate the environmental toxicity of a large number of chemicals. At the present study, the acute toxicity of 23 1,2,4-triazole fungicides to zebrafish (Danio rerio) embryos was firstly tested, and the LC (median lethal concentration) values were used as the bio-activity endpoint to conduct QSAR modelling for these triazoles. After the comparative study of several QSAR models, the 2D-QSAR model was finally constructed using the stepwise multiple linear regression algorithm combining with two physicochemical parameters (logD and μ), an electronic parameter (Q) and a topological parameter (XPC). The optimal model could be mathematically described as following: pLC = -7.24-0.30XPC + 0.76logD - 26.15Q - 0.08μ. The internal validation by leave-one-out (LOO) cross-validation showed that the R (adjusted noncross-validation squared correlation coefficient), Q (cross-validation correlation coefficient) and RMSD (root-mean-square error) was 0.88, 0.84 and 0.17, respectively. The external validation indicated the model had a robust predictability with the q (predictive squared correlation coefficient) of 0.90 when eliminated tricyclazole. The present study provided a potential tool for predicting the acute toxicity of new 1,2,4-triazole fungicides which contained an independent triazole ring group in their molecules to zebrafish embryos, and also provided a reference for the development of more environmentally-friendly 1,2,4-triazole pesticides in the future.
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http://dx.doi.org/10.1016/j.envpol.2020.114837DOI Listing
October 2020

Uniportal Thoracoscopic Wedge Resection of Lung Nodules: Paravertebral Blocks Are Better Than Intercostal Blocks.

Surg Innov 2020 Aug 19;27(4):358-365. Epub 2020 May 19.

The Second Affiliated Hospital, Southern University of Science and Technology, Shenzhen, China.

. Regional analgesia for tubeless, uniport, thoracoscopic wedge resection of benign peripheral nodules is generally performed by intercostal nerve block (INB). We examined the effectiveness of thoracic paravertebral block (PVB), in comparison to the traditional intercostal blocks, for the procedure. . Between July 2016 and December 2016, 20 consecutive patients with solitary benign peripheral lung nodules underwent tubeless uniport thoracoscopic wedge resection using thoracic PVB (PVB group). The clinical outcomes were compared with those of 20 other consecutive patients who underwent the same procedure under the conventional INB, between January 2016 and July 2016 (INB group). In both groups, the procedures were performed without endotracheal intubation, urinary catheterization, or chest tube drainage. . The clinical data of patients in both groups were comparable in terms of demographic and baseline characteristics, operative and anesthetic characteristics, puncture-related complications, and postoperative anesthetic adverse events. No puncture-related complications occurred during the perioperative period in either group. The threshold values for mechanical pain at postoperative hours 4 and 8 were significantly higher in the PVB group than in the INB group. Furthermore, the incidence of nausea or vomiting in the PVB group was significantly less than that in the INB group. None of the patients required reintervention or readmission to our hospital. . Tubeless uniportal thoracoscopic wedge resection for solitary benign peripheral lung nodules using thoracic PVB for regional analgesia is a feasible and safe procedure. Moreover, we found that thoracic PVB is less painful than INB.
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http://dx.doi.org/10.1177/1553350620921753DOI Listing
August 2020

The N-terminal and third transmembrane domain of PsCor413im1 are essential for targeting to chloroplast envelope membrane.

Biochem Biophys Res Commun 2020 07 16;527(4):929-934. Epub 2020 May 16.

College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin, 150030, China. Electronic address:

Cold-regulated (COR) genes, located downstream of the C-repeat binding factors (CBFs) in cold signaling pathways, play a central role in plant response to cold stress. In our previous studies, a Cor413 chloroplast envelope membrane protein, PsCor413im1, was identified from the cold-tolerant plant Phlox subulata. Its overexpression enhanced cold tolerance and altered AtCor15 expression in Arabidopsis. In the present study, the function of PsCor413im1 was further investigated. Transmission electron microscope observation showed that the chloroplast envelope membrane of cold-treated transgenic Arabidopsis seedlings was more stable than that of cold-treated wild-type seedlings. Subcellular localization of green fluorescent protein as a marker revealed that the N-terminal and putative third transmembrane domain (TMD) of PsCor413im1 were essential for its targeting of the chloroplast envelope membrane. Furthermore, overexpression of PsCor413im1 fragments containing N-terminal and third TMD also altered the expression of AtCor15 genes in Arabidopsis. Overall, our results suggest that PsCor413im1 may stabilize the chloroplast envelope membrane under cold stress, and its N-terminal and third TMD are important for its targeting capability and function.
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http://dx.doi.org/10.1016/j.bbrc.2020.05.046DOI Listing
July 2020

Early enteral feeding on esophageal cancer patients after esophageal resection and reconstruction.

Ann Palliat Med 2020 May 16;9(3):816-823. Epub 2020 Apr 16.

Department of Thoracic Surgery, Shenzhen Third People' s Hospital, Shenzhen 518112, China.

Background: In patients under esophagectomy, early postoperative oral feeding has traditionally been contraindicated to minimize the risk of anastomotic leaks. Because early oral feeding preserves the integrity and function of gut mucosa, the aim of this study was to investigate the impact of postoperative early oral feeding on esophagectomy.

Methods: Between Oct 2013 and Jan 2016, 41 consecutive patients with esophageal carcinoma (stages I- III), who had undergone minimally invasive Ivor-Lewis surgery, were enrolled in this study. The patients were randomly arranged into the early oral feeding (EOF) group (21 cases) and the simple tube feeding (STF) group (20 cases). The nutritional goal for both groups was 25 kcal/(kg·day). The patients in the EOF group were tube-fed with enteral nutrition and orally fed with 5% glucose in normal saline during the first 4 postoperative days, after that the patients were placed on a liquid diet. The patients in the STF group were postoperatively tube-fed with enteral nutrition for 7 days before being switched to liquid diet. The length of the postoperative hospital stay, rate of perioperative complications, and overall mortality were recorded. Preoperative and postoperative levels of serum albumin (ALB), prealbumin (PA), transferrin (TP), and C reactive protein (CRP) were also monitored.

Results: There were no significant differences of the incidence of postoperative complications and overall mortality between the EOF group and the STF group, but the duration of hospital stay, interval until the first liquid food, and time of ambulation in the EOF group were lower than those of the STF group (P<0.05). EOF patients also showed significantly lower CRP levels compared with the STF group (P<0.05).

Conclusions: EOF might reduce the duration of hospital stays and CRP levels in esophageal cancer patients who underwent esophagectomy while the mortality and complications were not affected.
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http://dx.doi.org/10.21037/apm.2020.04.13DOI Listing
May 2020

Ocular manifestations of a hospitalised patient with confirmed 2019 novel coronavirus disease.

Br J Ophthalmol 2020 06 7;104(6):748-751. Epub 2020 Apr 7.

Ophthalmology, Shenzhen Eye Hospital, Shenzhen, China.

Purpose: To report the ocular characteristics and the presence of viral RNA of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in conjunctival swab specimens in a patient with confirmed 2019 novel coronavirus disease (COVID-19).

Participant And Methods: A 30-year-old man with confirmed COVID-19 and bilateral acute conjunctivitis which occurred 13 days after illness onset. Based on detailed ophthalmic examination, reverse transcription PCR (RT-PCR) was performed to detect SARS-CoV-2 virus in conjunctival swabs. The ocular characteristics, presence of viral RNA and viral dynamics of SARS-CoV-2 in the conjunctival specimens were evaluated.

Results: Slit lamp examination showed bilateral acute follicular conjunctivitis. RT-PCR assay demonstrated the presence of viral RNA in conjunctival specimen 13 days after onset (cycle threshold value: 31). The conjunctival swab specimens remained positive for SARS-CoV-2 on 14 and 17 days after onset. On day 19, RT-PCR result was negative for SARS-CoV-2.

Conclusion: SARS-CoV-2 is capable of causing ocular complications such as viral conjunctivitis in the middle phase of illness. Precautionary measures are recommended when examining infected patients throughout the clinical course of the infection. However, conjunctival sampling might not be useful for early diagnosis because the virus may not appear initially in the conjunctiva.
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http://dx.doi.org/10.1136/bjophthalmol-2020-316304DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7211077PMC
June 2020

Lung transplantation as therapeutic option in acute respiratory distress syndrome for coronavirus disease 2019-related pulmonary fibrosis.

Chin Med J (Engl) 2020 Jun;133(12):1390-1396

Department of Thoracic Surgery/Oncology, State Key Laboratory and National Clinical Research Center for Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510120, China.

Background: Critical patients with the coronavirus disease 2019 (COVID-19), even those whose nucleic acid test results had turned negative and those receiving maximal medical support, have been noted to progress to irreversible fatal respiratory failure. Lung transplantation (LT) as the sole therapy for end-stage pulmonary fibrosis related to acute respiratory distress syndrome has been considered as the ultimate rescue therapy for these patients.

Methods: From February 10 to March 10, 2020, three male patients were urgently assessed and listed for transplantation. After conducting a full ethical review and after obtaining assent from the family of the patients, we performed three LT procedures for COVID-19 patients with illness durations of more than one month and extremely high sequential organ failure assessment scores.

Results: Two of the three recipients survived post-LT and started participating in a rehabilitation program. Pearls of the LT team collaboration and perioperative logistics were summarized and continually improved. The pathological results of the explanted lungs were concordant with the critical clinical manifestation, and provided insight towards better understanding of the disease. Government health affair systems, virology detection tools, and modern communication technology all play key roles towards the survival of the patients and their rehabilitation.

Conclusions: LT can be performed in end-stage patients with respiratory failure due to COVID-19-related pulmonary fibrosis. If confirmed positive-turned-negative virology status without organ dysfunction that could contraindicate LT, LT provided the final option for these patients to avoid certain death, with proper protection of transplant surgeons and medical staffs. By ensuring instant seamless care for both patients and medical teams, the goal of reducing the mortality rate and salvaging the lives of patients with COVID-19 can be attained.
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http://dx.doi.org/10.1097/CM9.0000000000000839DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7339336PMC
June 2020

Characteristics of Heat Resistant Aluminum Alloy Composite Core Conductor Used in overhead Power Transmission Lines.

Materials (Basel) 2020 Mar 31;13(7). Epub 2020 Mar 31.

Carbon Fiber Engineering Research Center, Shandong University, Jinan 250061, China.

The heat resistant aluminum alloy wire composite material core conductor (ACCC/HW) which was used in overhead transmission lines is developed and studied in this work. The composite material core is carbon fiber/glass cloth reinforced modified epoxy resin composite. Tensile stress tests and stress-strain tests of both composite core and conductor are taken at 25 °C and 160 °C. Sag test, creep test and current carrying capacity test of composite conductor are taken. The stress of composite conductor are 425.2 MPa and 366.9 MPa at 25 °C and 160 °C, respectively. The sag of conductor of 50 m length are 95 mm, 367 mm, and 371 mm at 25 °C, 110 °C, and 160 °C, respectively. The creep strain are 271 mm/km, 522 mm/km, and 867 mm/km after 10 years under the tension of 15% RTS (Rated Tensile Strength), 25% RTS and 35% RTS at 25 °C, and 628 mm/km under 25% RTS at 160 °C, according to the test result and calculation. The carrying capacity of composite conductor is basically equivalent to ACSR (Aluminum Conductor Steel Reinforced). ACCC/HW is suitable in overhead transmission lines, and it has been used in 50 kV power grid, according to the results.
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http://dx.doi.org/10.3390/ma13071592DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7178384PMC
March 2020

Cutting Edge: Characterization of Human Tissue-Resident Memory T Cells at Different Infection Sites in Patients with Tuberculosis.

J Immunol 2020 05 30;204(9):2331-2336. Epub 2020 Mar 30.

Institute of Molecular Immunology, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou 510515, China;

Tissue-resident memory T cells (TRMs) have a key role in mediating the host defense against tuberculosis (TB) in mice, but their human counterparts have not been well characterized. In this article, we recruited patients with TB and determined TRM frequency, trafficking, activation marker expression, and cytokine production by flow or mass cytometry at different infection sites, including peripheral blood, pleural fluid, bronchoalveolar lavage fluid, and lung. We found a high frequency of TRMs at all infection sites apart from the peripheral blood. These TRMs exhibited a memory phenotype, were highly activated (based on CD38 and HLA-DR expression), and expressed high levels of trafficking (CCR5 and CXCR6) and exhaustion (PD-1) markers. When stimulated with , TRMs secreted cytokines, including IFN-γ, TNF-α, and IL-2, and exhibited a multifunctional phenotype. TRMs limited intracellular replication in macrophages. These data inform our current understanding of immunosurveillance at different infection sites in patients with TB.
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http://dx.doi.org/10.4049/jimmunol.1901326DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7167459PMC
May 2020

Titanium dioxide nanoparticles enhanced thyroid endocrine disruption of pentachlorophenol rather than neurobehavioral defects in zebrafish larvae.

Chemosphere 2020 Jun 17;249:126536. Epub 2020 Mar 17.

State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academic of Science, Wuhan, 430072, China.

This study investigated the influences of titanium dioxide nanoparticles (n-TiO) on the thyroid endocrine disruption and neurobehavioral defects induced by pentachlorophenol (PCP) in zebrafish (Danio rerio). Embryos (2 h post-fertilization) were exposed to PCP (0, 3, 10, and 30 μg/L) or in combination with n-TiO (0.1 mg/L) until 6 days post-fertilization. The results showed that n-TiO alone did not affect thyroid hormones levels or transcriptions of related genes. Exposure to PCP significantly decreased thyroid hormone thyroxine (T4) content, thyroid stimulating hormone (TSH) level and transcription of thyroglobulin (tg), but significantly increased 3,5,3'-triiodothyronine (T3) level and upregulation of deiodinase 2 (dio2). In comparison, the co-exposure with n-TiO significantly reduced the content of T3 by depressing the potential targets, tg and dio2. For neurotoxicity, the single and co-exposure resulted in similar effects with significant downregulation of neurodevelopment-related genes (ELAV like RNA Binding Protein 3, elavl3; Growth associated protein-43, gap43; α-tubulin) and inhibited locomotor activity. The results indicated that the presence of n-TiO significantly enhanced the PCP-induced thyroid endocrine disruption but not the neurobehavioral defects in zebrafish larvae.
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http://dx.doi.org/10.1016/j.chemosphere.2020.126536DOI Listing
June 2020

Preparation of aminoalkyl-grafted bacterial cellulose membranes with improved antimicrobial properties for biomedical applications.

J Biomed Mater Res A 2020 05 22;108(5):1086-1098. Epub 2020 Jan 22.

School of Materials Science and Engineering, University of Science and Technology Beijing, Beijing, China.

Bacterial cellulose (BC) membranes display special properties and structures, thus attracting much attention in application in the biomedical areas, for example, as implants for bone or cartilage tissue engineering, as substitutes for skin repairing, and as supports for controlled drug delivery. However, native BC lacks the activity to inhibit bacteria growth on its surface, which limits its applications in biomedical fields. There have been reports on chemical modification of BC membranes to endow them with antimicrobial properties needed for some special biomedical applications. In the present study, aminoalkyl-grafted BC membranes were prepared by alkoxysilane polycondensation using 3-aminopropyltriethoxysilane (APTES). The characterization for morphology and chemical composition showed that BC membranes were successfully grafted with aminoalkylsilane groups through covalent bonding. The surface morphology and roughness of the membranes changed after chemical grafting. Furthermore, after grafting with APTES, the membranes got less hydrophilic than native BC. The aminoalkyl-grafted BC membranes showed strong antibacterial properties against Staphylococcus aureus and Escherichia coli and moreover, they were nontoxic to normal human dermal fibroblasts. These results indicate that aminoalkyl-grafted BC membranes are potential to be used for biomedical applications.
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http://dx.doi.org/10.1002/jbm.a.36884DOI Listing
May 2020

Feasibility of non-intubated anesthesia and regional block for thoracoscopic surgery under spontaneous respiration: a prospective cohort study.

Braz J Med Biol Res 2020 20;53(1):e8645. Epub 2019 Dec 20.

Department of Anesthesiology, Zhujiang Hospital of Southern Medical University, Guangzhou, Guangdong, China.

Data about the feasibility and safety of thoracoscopic surgery under non-intubated anesthesia and regional block are limited. In this prospective study, 57 consecutive patients scheduled for thoracoscopic surgery were enrolled. Patients were sedated with dexmedetomidine and anesthetized with propofol and remifentanil. Ropivacaine was used for intercostal nerve and paravertebral block. Lidocaine was used for vagal block. The primary outcomes were mean arterial pressure (MAP), heart rate (HR), oxygen saturation, and end-tidal carbon dioxide partial pressure (ETCO2) at T0 (pre-anesthesia), T1 (immediately after laryngeal mask/nasopharyngeal airway placement), T2 (immediately after skin incision), T3 (10 min after opening the chest), T4 (end of surgery), and T5 (immediately after laryngeal mask/nasopharyngeal airway removal). One patient required conversion to intubation, 15 developed intraoperative hypotension, and two had hypoxemia. MAP at T0 and T5 was higher than at T1-T4; MAP at T3 was lower (P<0.05 vs other time points). HR at T0 and T5 was higher (P<0.05 vs other time points). ETCO2 at T2 and T3 was higher (P<0.05 vs other time points). Arterial pH, PCO2, and lactic acid at T1 differed from values at T0 and T2 (P<0.05). The Quality of Recovery-15 (QoR-15) score at 24 h was lower (P<0.05). One patient experienced dysphoria during recovery. Thoracoscopic surgery with regional block under direct thoracoscopic vision is a feasible and safe alternative to conventional surgery under general anesthesia, intubation, and one-lung ventilation.
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http://dx.doi.org/10.1590/1414-431X20198645DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6915876PMC
February 2020