Publications by authors named "Kun Jiang"

327 Publications

Predicting persistent organ failure on admission in patients with acute pancreatitis: development and validation of a mobile nomogram.

HPB (Oxford) 2022 Jun 6. Epub 2022 Jun 6.

Department of Integrated Traditional Chinese and Western Medicine, Sichuan Provincial Pancreatitis Center and West China-Liverpool Biomedical Research Center, West China Hospital, Sichuan University, Chengdu, China. Electronic address:

Background: Early prediction of persistent organ failure (POF) is important for triage and timely treatment of patients with acute pancreatitis (AP).

Methods: All AP patients were consecutively admitted within 48 h of symptom onset. A nomogram was developed to predict POF on admission using data from a retrospective training cohort, validated by two prospective cohorts. The clinical utility of the nomogram was defined by concordance index (C-index), decision curve analysis (DCA), and clinical impact curve (CIC), while the performance by post-test probability.

Results: There were 816, 398, and 880 patients in the training, internal and external validation cohorts, respectively. Six independent predictors determined by logistic regression analysis were age, respiratory rate, albumin, lactate dehydrogenase, oxygen support, and pleural effusion and were included in the nomogram (web-based calculator: https://shina.shinyapps.io/DynNomapp/). This nomogram had reasonable predictive ability (C-indexes 0.88/0.91/0.81 for each cohort) and promising clinical utility (DCA and CIC). The nomogram had a positive likelihood ratio and post-test probability of developing POF in the training, internal and external validation cohorts of 4.26/31.7%, 7.89/39.1%, and 2.75/41%, respectively, superior or equal to other prognostic scores.

Conclusions: This nomogram can predict POF of AP patients and should be considered for clinical practice and trial allocation.
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http://dx.doi.org/10.1016/j.hpb.2022.05.1347DOI Listing
June 2022

ROS-sensitive calcipotriol nano-micelles prepared by methoxypolyethylene glycol (mPEG) - modified polymer for the treatment of psoriasis.

Drug Deliv 2022 Dec;29(1):1903-1913

School of Pharmacy, Weifang Medical University, Weifang, China.

Oxidative stress due to excessive reactive oxygen species (ROS) production in the skin microenvironment is one of the main mechanisms in psoriasis pathogenesis. A nano drug delivery system based on ROS-responsive release can enhance drug release at the target site. In this study, a ROS-sensitive material methoxypolyethylene glycol-thioether-thiol (mPEG-SS) was synthesized using mPEG as the parent structure with sulfide structural modification. An mPEG-SS-calcipotriol (mPEG-SS-CPT, PSC) nano-micelle percutaneous delivery system was prepared by encapsulating CPT. A small animal imaging system was used to study PSC's the ROS-sensitive drug release process. It is shown that endogenous ROS mainly affects PSC and releases drugs. Finally, the therapeutic effect of PSC on psoriasis was explored by animal experiments. Ultimately, it ameliorates imiquimod-induced psoriasis-like inflammation. Overall, PSC is an effective ROS-sensitive transdermal drug delivery system that is expected to provide a new strategy for treating psoriasis.
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http://dx.doi.org/10.1080/10717544.2022.2086944DOI Listing
December 2022

Template Guided Regioselective Encaging of Platinum Single Atoms into Y Zeolite: Enhanced Selectivity in Semihydrogenation and Resistance to Poisoning.

Angew Chem Int Ed Engl 2022 Jun 9. Epub 2022 Jun 9.

Shenzhen University, College of Materials Science and Engineering, CHINA.

It is challenging to localize single metal atoms at target sites of zeolite with uniform coordination environment. In this study, single platinum atoms were selectively encaged into six-membered rings of sodalite (SOD) cages within Y zeolite through a template guiding strategy, in which, during in situ synthesis process, template molecules were designed to occupy supercages, thus to enforce coordinated platinum species involved into SOD cages, and then, followed by control of post-treatment condition to obtain this Y zeolite selectively encaged single platinum atoms catalyst (denoted as [email protected]). In addition to show good stability and excellent catalytic selectivity in semihydrogenation reaction, [email protected] catalyst also exhibited interesting thiophene-resistance and carbon monoxide-resistance abilities in this transformation, as a result of the unique configuration of encaged single platinum atoms to weaken the interactions with these poisons.
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http://dx.doi.org/10.1002/anie.202205978DOI Listing
June 2022

Discriminability of single-trial EEG during decision-making of cooperation or aggression: a study based on machine learning.

Med Biol Eng Comput 2022 Jun 6. Epub 2022 Jun 6.

School of Economics and Management, Fuzhou University, Fuzhou, 350108, China.

Decision-making is a very important cognitive process in our daily life. There has been increasing interest in the discriminability of single-trial electroencephalogram (EEG) during decision-making. In this study, we designed a machine learning based framework to explore the discriminability of single-trial EEG corresponding to different decisions. For each subject, the framework split the decision-making trials into two parts, trained a feature model and a classifier on the first part, and evaluated the discriminability on the second part using the feature model and classifier. A proposed algorithm and five existing algorithms were applied to fulfill the feature models, and the algorithm Linear Discriminative Analysis (LDA) was used to implement the classifiers. We recruited 21 subjects to participate in Chicken Game (CG) experiments. The results show that there exists the discriminability of single-trial EEG between the cooperation and aggression decisions during the CG experiments, with the classification accuray of 75% (±6%), and the discriminability is mainly from the EEG information below 40 Hz. The further analysis indicates that the contributions of different brain regions to the discriminability are consistent with the existing knowledge on the cognitive mechanism of decision-making, confirming the reliability of the conclusions. This study exhibits that it is feasible to apply machine learning methods to EEG analysis of decision-making cognitive process.
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http://dx.doi.org/10.1007/s11517-022-02557-5DOI Listing
June 2022

A New Species in Hsiao in China Confirmed by Morphological and Molecular Data (Hemiptera: Heteroptera: Coreidae).

Insects 2022 Apr 26;13(5). Epub 2022 Apr 26.

College of Life Sciences, Nankai University, Tianjin 300071, China.

To date, only one species of genus , Hsiao, was found in Yunnan, China. Recently, we confirmed a new species, ., by morphological and molecular data based on new specimens collected from Yunnan, China. The new species is morphologically similar to except for the male genitalia and the sternum of the seventh abdominal segment of the female. The extraordinary difference of the genitalia between these two species inspired us to erect a new genus for this new species. However, considering their sister group relationship to other genera in tribe Cloresmini and the 12.56~12.64% genetic distance (meeting the interspecific genetic distance within genus of tribe Cloresmini) by a complete COI gene, this species was more reasonable as a new species of the genus , and the diagnosis of the genus was revised. The photos of the body and critical morphological characteristics for both male and female were provided for accurate identification.
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http://dx.doi.org/10.3390/insects13050411DOI Listing
April 2022

Dissolvable microgel-templated macroporous hydrogels for controlled cell assembly.

Mater Sci Eng C Mater Biol Appl 2022 Feb 14:112712. Epub 2022 Feb 14.

Department of Biomedical Engineering, Indiana University-Purdue University Indianapolis, Indianapolis, IN 46202, USA; Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USA; Indiana University Simon Comprehensive Cancer Center, Indianapolis, IN 46202, USA. Electronic address:

Mesenchymal stem cells (MSCs)-based therapies have been widely used to promote tissue regeneration and to modulate immune/inflammatory response. The therapeutic potential of MSCs can be further improved by forming multi-cellular spheroids. Meanwhile, hydrogels with macroporous structures are advantageous for improving mass transport properties for the cell-laden matrices. Herein, we report the fabrication of MSC-laden macroporous hydrogel scaffolds through incorporating rapidly dissolvable spherical cell-laden microgels. Dissolvable microgels were fabricated by tandem droplet-microfluidics and thiol-norbornene photopolymerization using a novel fast-degrading macromer poly(ethylene glycol)-norbornene-dopamine (PEGNB-Dopa). The cell-laden PEGNB-Dopa microgels were subsequently encapsulated within another bulk hydrogel matrix, whose porous structure was generated efficiently by the rapid degradation of the PEGNB-Dopa microgels. The cytocompatibility of this in situ pore-forming approach was demonstrated with multiple cell types. Furthermore, adjusting the stiffness and cell adhesiveness of the bulk hydrogels afforded the formation of solid cell spheroids or hollow spheres. The assembly of solid or hollow MSC spheroids led to differential activation of AKT pathway. Finally, MSCs solid spheroids formed in situ within the macroporous hydrogels exhibited robust secretion of HGF, VEGF-A, IL-6, IL-8, and TIMP-2. In summary, this platform provides an innovative method for forming cell-laden macroporous hydrogels for a variety of future biomedical applications.
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http://dx.doi.org/10.1016/j.msec.2022.112712DOI Listing
February 2022

Hyperphosphorylated tau self-assembles into amorphous aggregates eliciting TLR4-dependent responses.

Nat Commun 2022 May 16;13(1):2692. Epub 2022 May 16.

Department of Chemistry, University of Cambridge, Cambridge, UK.

Soluble aggregates of the microtubule-associated protein tau have been challenging to assemble and characterize, despite their important role in the development of tauopathies. We found that sequential hyperphosphorylation by protein kinase A in conjugation with either glycogen synthase kinase 3β or stress activated protein kinase 4 enabled recombinant wild-type tau of isoform 0N4R to spontaneously polymerize into small amorphous aggregates in vitro. We employed tandem mass spectrometry to determine the phosphorylation sites, high-resolution native mass spectrometry to measure the degree of phosphorylation, and super-resolution microscopy and electron microscopy to characterize the morphology of aggregates formed. Functionally, compared with the unmodified aggregates, which require heparin induction to assemble, these self-assembled hyperphosphorylated tau aggregates more efficiently disrupt membrane bilayers and induce Toll-like receptor 4-dependent responses in human macrophages. Together, our results demonstrate that hyperphosphorylated tau aggregates are potentially damaging to cells, suggesting a mechanism for how hyperphosphorylation could drive neuroinflammation in tauopathies.
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http://dx.doi.org/10.1038/s41467-022-30461-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9110413PMC
May 2022

Nitrogen removal characteristics of biofilms in each area of a full-scale AAO oxidation ditch process.

Chemosphere 2022 Sep 9;302:134871. Epub 2022 May 9.

School of Water Conservancy and Engineering, Zhengzhou University, Zhengzhou, 450001, China.

Plastic carriers were installed in different areas of a full-scale anaerobic/anoxic/aerobic (AAO) oxidation ditch process, and the dynamics of nitrogen removal, biofilm morphologies, and microorganism species were investigated. The results showed that the biofilm at the front of the aerobic area (dissolved oxygen [DO] = 0.93 mg L) provided the best denitrification, with specific nitrate and nitrite reduction rates of 10.16 and 3.78 mg·(g·h), respectively. The biofilm in the middle of the aerobic area (DO = 1.27 mg L) exhibited the best nitrification performance, with a maximum specific ammonia oxidation rate of 3.21 mg·(g·h). The biofilm at the end of the aerobic area (DO = 0.01 mg L) exhibited the highest anammox potential with a maximum specific anammox rate of 0.67 mg·(g·h). No correlation was observed between the specific nitrogen removal rates and abundance of nitrogen-removing microorganisms at the genus level. Biofilm denitrification during the process was primarily completed by heterotrophic denitrifying bacteria (Thauera, Acinetobacter, Hyphomicrobium, and Thermomonas). Aerobic denitrifying bacteria (0.19% Thauera and 0.34% Hyphomicrobium) were identified as the main denitrifying bacteria in the middle of the aerobic area. The dominant nitrifying microorganisms in the middle of the aerobic area were Nitrosomonas (0.50%) and Nitrospira (1.04%). A biofilm in the end of the aerobic area exhibited specific anammox potential, which may have been related to the dominance of 0.024% Candidatus Brocadia. Kinetic analysis revealed that adding plastic carriers to the front and middle of the aerobic area contributed to stable nitrogen removal efficiency.
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http://dx.doi.org/10.1016/j.chemosphere.2022.134871DOI Listing
September 2022

Construction of an Assisted Model Based on Natural Language Processing for Automatic Early Diagnosis of Autoimmune Encephalitis.

Neurol Ther 2022 May 11. Epub 2022 May 11.

Department of Neurology, Xijing Hospital, Fourth Military Medical University, Xi'an, China.

Introduction: Early diagnosis and etiological treatment can effectively improve the prognosis of patients with autoimmune encephalitis (AE). However, anti-neuronal antibody tests which provide the definitive diagnosis require time and are not always abnormal. By using natural language processing (NLP) technology, our study proposes an assisted diagnostic method for early clinical diagnosis of AE and compares its sensitivity with that of previously established criteria.

Methods: Our model is based on the text classification model trained by the history of present illness (HPI) in electronic medical records (EMRs) that present a definite pathological diagnosis of AE or infectious encephalitis (IE). The definitive diagnosis of IE was based on the results of traditional etiological examinations. The definitive diagnosis of AE was based on the results of neuronal antibodies, and the diagnostic criteria of definite autoimmune limbic encephalitis proposed by Graus et al. used as the reference standard for antibody-negative AE. First, we automatically recognized and extracted symptoms for all HPI texts in EMRs by training a dataset of 552 cases. Second, four text classification models trained by a dataset of 199 cases were established for differential diagnosis of AE and IE based on a post-structuring text dataset of every HPI, which was completed using symptoms in English language after the process of normalization of synonyms. The optimal model was identified by evaluating and comparing the performance of the four models. Finally, combined with three typical symptoms and the results of standard paraclinical tests such as cerebrospinal fluid (CSF), magnetic resonance imaging (MRI), or electroencephalogram (EEG) proposed from Graus criteria, an assisted early diagnostic model for AE was established on the basis of the text classification model with the best performance.

Results: The comparison results for the four models applied to the independent testing dataset showed the naïve Bayesian classifier with bag of words achieved the best performance, with an area under the receiver operating characteristic curve of 0.85, accuracy of 84.5% (95% confidence interval [CI] 74.0-92.0%), sensitivity of 86.7% (95% CI 69.3-96.2%), and specificity of 82.9% (95% CI 67.9-92.8%), respectively. Compared with the diagnostic criteria proposed previously, the early diagnostic sensitivity for possible AE using the assisted diagnostic model based on the independent testing dataset was improved from 73.3% (95% CI 54.1-87.7%) to 86.7% (95% CI 69.3-96.2%).

Conclusions: The assisted diagnostic model could effectively increase the early diagnostic sensitivity for AE compared to previous diagnostic criteria, assist physicians in establishing the diagnosis of AE automatically after inputting the HPI and the results of standard paraclinical tests according to their narrative habits for describing symptoms, avoiding misdiagnosis and allowing for prompt initiation of specific treatment.
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http://dx.doi.org/10.1007/s40120-022-00355-7DOI Listing
May 2022

The Kelch-F-box protein SMALL AND GLOSSY LEAVES 1 (SAGL1) negatively influences salicylic acid biosynthesis in Arabidopsis thaliana by promoting the turn-over of transcription factor SYSTEMIC ACQUIRED RESISTANCE DEFICIENT 1 (SARD1).

New Phytol 2022 May 1. Epub 2022 May 1.

State Key Laboratory of Crop Stress Adaptation and Improvement, Henan Joint International Laboratory for Crop Multi-Omics Research, School of Life Sciences, Henan University, Jinming Road, Kaifeng, 475004, China.

Salicylic acid (SA) is a key phytohormone regulating plant immunity. Although the transcriptional regulation of SA biosynthesis has been well-studied, its post-translational regulation is largely unknown. We report that a Kelch repeats-containing F-box (KFB) protein, SMALL AND GLOSSY LEAVES 1 (SAGL1), negatively influences SA biosynthesis in Arabidopsis thaliana by mediating the proteolytic turnover of SYSTEMIC ACQUIRED RESISTANCE DEFICIENT 1 (SARD1), a master transcription factor that directly drives SA biosynthesis during immunity. Loss of SAGL1 resulted in characteristic growth inhibition. Combining metabolomic, transcriptional and phenotypic analyses, we found that SAGL1 represses SA biosynthesis and SA-mediated immune activation. Genetic crosses to mutants that are deficient in SA biosynthesis blocked the SA overaccumulation in sagl1 and rescued its growth. Biochemical and proteomic analysis identified that SAGL1 interacts with SARD1 and promotes the degradation of SARD1 in a proteasome-dependent manner. These results unravelled a critical role of KFB protein SAGL1 in maintaining SA homeostasis via controlling SARD1 stability.
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http://dx.doi.org/10.1111/nph.18197DOI Listing
May 2022

Climate Warming Since the Holocene Accelerates West-East Communication for the Eurasian Temperate Water Strider Species Aquarius paludum.

Mol Biol Evol 2022 May;39(5)

Institute of Entomology, College of Life Sciences, Nankai University, 94 Weijin Road, Tianjin 300071, China.

Holocene climate warming has dramatically altered biological diversity and distributions. Recent human-induced emissions of greenhouse gases will exacerbate global warming and thus induce threats to cold-adapted taxa. However, the impacts of this major climate change on transcontinental temperate species are still poorly understood. Here, we generated extensive genomic datasets for a water strider, Aquarius paludum, which was sampled across its entire distribution in Eurasia and used these datasets in combination with ecological niche modeling (ENM) to elucidate the influence of the Holocene and future climate warming on its population structure and demographic history. We found that A. paludum consisted of two phylogeographic lineages that diverged in the middle Pleistocene, which resulted in a "west-east component" genetic pattern that was probably triggered by Central Asia-Mongoxin aridification and Pleistocene glaciations. The diverged western and eastern lineages had a second contact in the Holocene, which shaped a temporary hybrid zone located at the boundary of the arid-semiarid regions of China. Future predictions detected a potentially novel northern corridor to connect the western and eastern populations, indicating west-east gene flow would possibly continue to intensify under future warming climate conditions. Further integrating phylogeographic and ENM analyses of multiple Eurasian temperate taxa based on published studies reinforced our findings on the "west-east component" genetic pattern and the predicted future northern corridor for A. paludum. Our study provided a detailed paradigm from a phylogeographic perspective of how transcontinental temperate species differ from cold-adapted taxa in their response to climate warming.
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http://dx.doi.org/10.1093/molbev/msac089DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9087890PMC
May 2022

Influences of a standardized food matrix and gastrointestinal fluids on the physicochemical properties of titanium dioxide nanoparticles.

RSC Adv 2021 Mar 19;11(19):11568-11582. Epub 2021 Mar 19.

School of Medical Instrument and Food Engineering, Shanghai Engineering Research Center for Food Rapid Detection, University of Shanghai for Science and Technology Shanghai 200093 China

The fast-growing applications of engineered titanium dioxide nanoparticles (e-TiO-NPs) in the food and pharmaceutical industry in production, packaging, sensors, nutrient delivery systems, and food additives enhance the possibility of oral exposure. Physicochemical transformations may occur when e-TiO-NPs are incorporated into a food matrix and pass through the human gastrointestinal tract (GIT), which may redefine the toxic effects of the e-TiO-NPs. In this study, a standardized food model (SFM) and simulated gastrointestinal fluids have been used to study the fate of e-TiO-NPs following a three-step digestion model , and a case study was carried out to assess the toxicity of the digested e-TiO-NPs using an cellular model. In the absence and presence of the SFM, the transformations of the tristimulus color coordinates, size, agglomeration state, surface charge and solubility of the e-TiO-NPs in the salivary, gastric and intestinal digestion fluids were compared with those before digestion. The results demonstrate that the presence of the SFM impacted the physicochemical properties of the e-TiO-NPs significantly. The SFM stabilized the e-TiO-NP suspensions and acted as a dispersant during each digestive phase. The e-TiO-NPs showed differentiated transformations of their physicochemical properties after each step of the digestive process. The pH shifts and variable concentrations of enzymes and salts in gastrointestinal fluids induced the transformations of the physicochemical properties of the e-TiO-NPs. The transformed e-TiO-NPs could release titanium ion in the gastrointestinal tract. Also, the cell viability induced by e-TiO-NPs was found to be strongly affected by the presence of the SFM and simulated human GIT fluids. It can be concluded that the physicochemical transformations of the e-TiO-NPs that were found when they were incorporated into an SFM and passed through the GIT consequently strongly affected the biological effects of the e-TiO-NPs, which highlights that the toxicity assessment of ingested NPs should use appropriate standardized food models and take realistic physiological conditions into account.
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http://dx.doi.org/10.1039/d0ra09706cDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8695913PMC
March 2021

PINCH-1 promotes IGF-1 receptor expression and skin cancer progression through inhibition of the GRB10-NEDD4 complex.

Theranostics 2022 28;12(6):2613-2630. Epub 2022 Feb 28.

Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA.

Insulin-like growth factor 1 receptor (IGF-1R) expression and signaling play important roles in promotion of skin cancer progression. Identification of signaling pathways that regulate IGF-1R is crucial for understanding the pathogenesis and therapeutic treatment of skin cancer. Molecular, cellular and genetic approaches were used to investigate the function of PINCH-1 in regulation of IGF-1R expression and skin cell behavior. Furthermore, conditional PINCH-1 knockout mouse and carcinogen (7, 12-dimethylbenz[a]anthracene (DMBA)/12-O-tetradecanoylphorbol-13-acetate (TPA))-induced skin cancer model were employed to determine the function of PINCH-1 in regulation of IGF-1R expression and skin carcinogenesis . Knockdown of PINCH-1 from HaCaT keratinocytes or A431 squamous carcinoma cells diminished IGF-1R levels, suppressed cell proliferation and increased apoptosis. Re-expression of PINCH-1 in PINCH-1 knockdown cells restored IGF-1R expression, cell proliferation and survival. Furthermore, depletion of NEDD4 effectively reversed PINCH-1 deficiency-induced down-regulation of IGF-1R expression, cell proliferation and survival. Conditional knockout of PINCH-1 from keratin 5 (K5) positive keratinocytes in mice, like depletion of PINCH-1 from keratinocytes in culture, reduced the IGF-1R level. Using a mouse model of DMBA/TPA-induced skin cancer, we show that the levels of both PINCH-1 and IGF-1R were significantly increased in response to treatment with the carcinogens. Genetic ablation of PINCH-1 from the epidermis markedly reduced the IGF-1R expression and cell proliferation despite stimulation with DMBA/TPA, resulting in resistance to chemical carcinogen-induced skin cancer initiation and progression. Our results reveal a PINCH-1-NEDD4-IGF-1R signaling axis that is critical for promotion of skin tumorigenesis and suggest a new strategy for therapeutic control of skin cancer progression.
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http://dx.doi.org/10.7150/thno.70744DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8965486PMC
February 2022

Bio-C (Modified Hyaluronic Acid-Coated-Collagen Tube) Implants Enable Functional Recovery after Complete Spinal Cord Injury.

Pharmaceutics 2022 Mar 9;14(3). Epub 2022 Mar 9.

Stem Cell Translational Research Center, Tongji Hospital, School of Medicine, Tongji University, Shanghai 200065, China.

Neural repair within the central nervous system (CNS) has been extremely challenging due to limited abilities of adult CNS neurons to regenerate, particularly in a highly inflammatory injury environment that is also filled with myelin debris. Spinal cord injury (SCI) is a serious medical condition that often leads to paralysis and currently has no effective treatment. Here we report the construction of a novel biocompatible and biodegradable material, Bio-C, through coating of acid-desalted-collagen (ADC) tube with pre-modified hyaluronic acid, which, after implantation, can elicit quite robust neural regeneration and functional recovery after complete spinal-cord transection with a 2 mm-spinal-cord-segment removal in mice. We combined morphological, electrophysiological, and objective transcriptomic analyses, in addition to behavioral analyses, to demonstrate neural tissue regeneration and functional recovery through the establishment of Bio-C-induced anti-inflammatory, neurogenic, and neurotrophic microenvironment. Through this study, we unveiled the underlying logic for CNS neural repair.
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http://dx.doi.org/10.3390/pharmaceutics14030596DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8954105PMC
March 2022

Deconstructive Insertion of Oximes into Coumarins: Modular Synthesis of Dihydrobenzofuran-Fused Pyridones.

Org Lett 2022 04 23;24(12):2282-2287. Epub 2022 Mar 23.

School of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, China.

In the presence of a copper catalyst, a series of oximes undergo deconstructive insertion into coumarins to afford structurally interesting dihydrobenzofuran-fused pyridones in moderate to good yields with good functional group compatibility. The reaction likely involves a radical relay annulation, leading to the ring opening of the lactone moiety of the coumarins, and simultaneous formation of three new bonds. The investigation of photoluminescent properties reveals that several obtained compounds may have potential as fluorescent materials.
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http://dx.doi.org/10.1021/acs.orglett.2c00384DOI Listing
April 2022

Nanoparticle encapsulated CQ/TAM combination harmonizes with MSCs in arresting progression of severity in AP mice through iNOS (IDO) signaling.

Mater Today Bio 2022 Mar 26;14:100226. Epub 2022 Feb 26.

State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, 6110041, China.

Background: Sever acute pancreatitis (SAP) is a critical disease with high mortality, and lack of clinically available treatments with specificity and effectiveness. Bone marrow derived mesenchymal stem cells (BMSCs) exhibited moderate effect on AP which needs further improvement.

Methods: Pancreatic infiltrating lymphocytes were analyzed to demonstrate the intervention of BMSCs on inflammatory cell infiltration of AP. Gene silencing with siRNA and small molecule inhibitor were utilized to determine the key effector molecule of BMSCs on AP. Pharmacological regulation and nanotechnology were introduced to further ameliorate BMSCs action.

Results: It was revealed that BMSCs prevent the progression of acute pancreatitis (AP) by reducing recruitment of macrophages, neutrophils and CD4+T cells in the lesion site. The pivotal role of chemokine-iNOS-IDO axis for BMSCs to intervene AP was confirmed. Compared with any single drug, Chloroquine/Tamoxifen combination together with IFN-γ pronouncedly up-regulated the transcription of several MSC immune regulators such as COX-2, PD-L1, HO-1 especially iNOS/IDO. As expected, BMSCs and human umbilical cord mesenchymal stem cells (UMSCs) pretreated with CQ/TAM/IFN-γ exerted enhanced intervention in AP and SAP mice. Moreover, pretreatment with CQ-LPs/TAM-NPs combination not only counteracted MSCs proliferation inhibition induced by free drugs but also enhanced their efficacy.

Conclusion: Under the background of rapid progress in MSCs clinical translation, this study focuses on the urgent clinical issue and initiates an original mechanism-based strategy to promote intervention on severity progression of SAP, which promises its clinical translation in future.
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http://dx.doi.org/10.1016/j.mtbio.2022.100226DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8924312PMC
March 2022

Defining health data elements under the HL7 development framework for metadata management.

J Biomed Semantics 2022 03 18;13(1):10. Epub 2022 Mar 18.

Institute for Health Informatics, Department of Health Statistics, the Ministry of Education Key Lab of Hazard Assessment and Control in Special Operational Environment, School of Public Health, Fourth Military Medical University, 169 Changle West Road, Xi'an, 710032, China.

Background: Health data from different specialties or domains generallly have diverse formats and meanings, which can cause semantic communication barriers when these data are exchanged among heterogeneous systems. As such, this study is intended to develop a national health concept data model (HCDM) and develop a corresponding system to facilitate healthcare data standardization and centralized metadata management.

Methods: Based on 55 data sets (4640 data items) from 7 health business domains in China, a bottom-up approach was employed to build the structure and metadata for HCDM by referencing HL7 RIM. According to ISO/IEC 11179, a top-down approach was used to develop and standardize the data elements.

Results: HCDM adopted three-level architecture of class, attribute and data type, and consisted of 6 classes and 15 sub-classes. Each class had a set of descriptive attributes and every attribute was assigned a data type. 100 initial data elements (DEs) were extracted from HCDM and 144 general DEs were derived from corresponding initial DEs. Domain DEs were transformed by specializing general DEs using 12 controlled vocabularies which developed from HL7 vocabularies and actual health demands. A model-based system was successfully established to evaluate and manage the NHDD.

Conclusions: HCDM provided a unified metadata reference for multi-source data standardization and management. This approach of defining health data elements was a feasible solution in healthcare information standardization to enable healthcare interoperability in China.
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http://dx.doi.org/10.1186/s13326-022-00265-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8932333PMC
March 2022

Development of a Nomogram for Predicting Refractory Pneumonia in Children.

Front Pediatr 2022 25;10:813614. Epub 2022 Feb 25.

Department of Pediatric Respiratory Medicine, Tianjin Children's Hospital, Tianjin University Children's Hospital, Tianjin, China.

Background: In children, refractory pneumonia (RMPP) may result in severe complications and high medical costs. There is research on a simple and easy-to-use nomogram for early prediction and timely treatment of RMPP.

Methods: From December 2018 to June 2021, we retrospectively reviewed medical records of 299 children with pneumonia (MPP) hospitalized in Tianjin Children's Hospital. According to their clinical manifestations, patients were divided into the RMPP group and the general pneumonia (GMPP) group. The clinical manifestations, laboratory indicators, and radiological data of the two groups were obtained. Stepwise regression was employed for variable selection of RMPP. The predictive factors selected were used to construct a prediction model which presented with a nomogram. The performance of the prediction model was evaluated by C statistics, calibration curve, and receiver operating characteristic (ROC) curve.

Results: The RMPP group significantly showed a higher proportion of females, longer fever duration, and longer hospital stay than the GMPP group ( < 0.05). Additionally, the RMPP group revealed severe clinical characteristics, including higher incidences of extrapulmonary complications, decreased breath sounds, unilateral pulmonary consolidation >2/3, and plastic bronchitis than the GMPP group ( < 0.05). The RMPP group had higher neutrophil ratio (N%), C-reactive protein (CRP), interleukin-6 (IL-6), lactic dehydrogenase (LDH), and D-dimer than the GMPP group ( < 0.05). Stepwise regression demonstrated that CRP [OR = 1.075 (95% CI: 1.020-1.133), < 0.001], LDH [OR = 1.015 (95% CI: 1.010-1.020), < 0.001], and D-dimer [OR = 70.94 (95% CI: 23.861-210.904), < 0.001] were predictive factors for RMPP, and developed a prediction model of RMPP, which can be visualized and accurately quantified using a nomogram. The nomogram showed good discrimination and calibration. The area under the ROC curve of the nomogram was 0.881, 95% CI (0.843, 0.918) in training cohorts and 0.777, 95% CI (0.661, 0.893) in validation cohorts, respectively.

Conclusion: C-reactive protein, LDH, and D-dimer were predictive factors for RMPP. The simple and easy-to-use nomogram assisted us in quantifying the risk for predicting RMPP, and more accurately and conveniently guiding clinicians to recognize RMPP, and contribute to a rational therapeutic choice.
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http://dx.doi.org/10.3389/fped.2022.813614DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8916609PMC
February 2022

One-year incidence rate of Treatment Resistant Depression (TRD) and treatment characteristics in China.

J Affect Disord 2022 05 28;305:77-84. Epub 2022 Feb 28.

Peking University Sixth Hospital, Peking University Institute of Mental Health, NHC Key Laboratory of Mental Health (Peking University), National Clinical Research Center for Mental Disorders (Peking University Sixth Hospital), Beijing, China. Electronic address:

Background: Little is known about the characteristics of Treatment-Resistant Depression (TRD) in China. In previous studies various identification approaches have led to a wide range of results, and it is unclear how Chinese patients compare to those in other studies.

Methods: This is a retrospective cohort study using electronic health records (EHR) from two major psychiatric hospitals in China. Adult major depressive disorder (MDD) patients who initiated pharmaceutical treatment during 2010-2018 were enrolled and follow-up was 1 year. TRD was primarily identified by consensus definition of two failures of adequate (≥4 weeks) regimens. Alternative regimens of 2-weeks and 6-weeks duration, and a data-driven definition were also applied.

Results: In the two hospitals, 12,257 (mean age: 40.8y, 63.6% female) and 8314 (mean age: 42.4y, 68.4% female) eligible patients were included. The 1-year incidence rate of TRD was estimated to be 5.2%-7.7% using the primary definition. TRD patients had mean treatment duration of 302.5 days and 285.7 days; had 3.6 and 3.7 treatment steps on average; 94.0% and 72.6% were prescribed polypharmacy regimens, which were all marginally greater than that of non-TRD patients. Alternative definitions resulted in a wide range of incidence estimates (0.5%-20.0%).

Limitations: Medications were assumed to be consumed as prescribed and lack of rating scales from EHRs may limit our TRD identification.

Conclusions: The incidence of TRD among Chinese MDD patients was comparable to other countries under similar settings and more complex treatment characteristics were observed among TRD patients. Alternative TRD definitions revealed the need for better treatment management in practices.
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http://dx.doi.org/10.1016/j.jad.2022.02.054DOI Listing
May 2022

Long-acting CoAlO spinel catalyst developed on activated alumina pellets by facile synthesis to activate peroxymonosulfate: Controllable cobalt leaching and environmental adaptability.

J Environ Manage 2022 May 17;310:114702. Epub 2022 Feb 17.

School of Minerals Processing and Bioengineering, Central South University, Changsha, 410083, China; Hunan Key Laboratory of Mineral Materials and Application, Central South University, Changsha, 410083, China.

A novel composite catalyst prepared by fixing cobalt aluminate (CoAlO) spinel on formed alumina carrier by impregnation-calcination route is reported, which can be used to efficiently activate peroxymonosulfate (PMS) to degrade p-nitrophenol (PNP). The internal laws of phase composition and preparation conditions are explored in detail, and the results show that the introduction of additional aluminum ions in the preparation process changes the coordination environment and the electronic state of cobalt ions, which leads to the transformation of spinel/inverted spinel in the composition, and further affects the activity and stability of the catalyst. The selected CoAl-Aaps-600 catalyst has high CoAlO content, showing good cycle performance and low cobalt leaching, and has great catalytic degradation performance at different temperatures and a wide pH range. Most notably, a fixed bed reactor packed with 20 g of CoAl-Aaps-600 exhibits excellent capacity to continuously treat 60 L of PNP solution with acceptable PNP removal ratio and low cobalt leaching content. Sulfate radical and singlet oxygen are identified as the main reactive oxygen species produced in CoAl-Aaps-600/PMS system, and the reaction mechanism is reasonably inferred. This work provides a potential application material and process for the treatment of continuous organic wastewater.
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http://dx.doi.org/10.1016/j.jenvman.2022.114702DOI Listing
May 2022

Application of fractional exhaled nitric oxide and nasal nitric oxide in control evaluation of bronchial asthma and diagnosis of allergic rhinitis in children.

Zhongguo Dang Dai Er Ke Za Zhi 2022 Jan;24(1):90-95

Department of Pulmonology, Shanghai Children's Hospital Affiliated to Shanghai Jiao Tong University, Shanghai 200062, China.

Objectives: To study the association of fractional exhaled nitric oxide (FeNO) and nasal nitric oxide (nNO) with asthma control and their value in the diagnosis of allergic rhinitis in children.

Methods: A total of 186 children aged 5-12 years, who attended the outpatient service of the Department of Respiration, Shanghai Children's Hospital due to bronchial asthma and/or allergic rhinitis or who underwent physical examination, were enrolled as subjects, with 52 children in the asthma group, 60 children in the asthma+allergic rhinitis group, 36 children in the allergic rhinitis group, and 38 children in the control group. FeNO, nNO, and pulmonary function were compared between groups.

Results: The asthma+allergic rhinitis, asthma, and allergic rhinitis groups had a significantly higher level of FeNO than the control group (<0.05). The asthma+allergic rhinitis and allergic rhinitis groups had a significantly higher level of nNO than the asthma and control groups (<0.05). The uncontrolled asthma and partially controlled asthma groups had significantly higher levels of FeNO and nNO than the completely controlled asthma group (<0.05). The receiver operating characteristic (ROC) curve analysis showed that nNO had an area under the ROC curve of 0.91, with a sensitivity of 80.0% and a specificity of 89.5% in the diagnosis of allergic rhinitis in children with asthma (<0.05).

Conclusions: The combined measurement of nNO and FeNO can be used to evaluate the control of asthma, and the measurement of nNO can help with the diagnosis of allergic rhinitis in children with bronchial asthma.
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http://dx.doi.org/10.7499/j.issn.1008-8830.2108091DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8802386PMC
January 2022

Neoadjuvant Nivolumab and Chemotherapy in Patients with Locally Advanced Non-Small Cell Lung Cancer: A Retrospective Study.

Cancer Manag Res 2022 9;14:515-524. Epub 2022 Feb 9.

Department of Thoracic Surgery, Xi'an International Medical Center Hospital, Xi'an, Shaanxi, 710000, People's Republic of China.

Background: Patients with locally advanced (stage III) non-small cell lung cancer (NSCLC) demonstrate broad anatomic heterogeneity with modest survival benefits. Immune checkpoint inhibitors (ICIs) have shown survival benefit in metastatic NSCLC. We conducted this study to evaluate the efficacy and safety of neoadjuvant nivolumab in combination with chemotherapy in the treatment of this population.

Methods: We retrospectively evaluated patients with locally advanced NSCLC receiving neoadjuvant nivolumab and chemotherapy (paclitaxel with carboplatin) followed by surgery at our institution from January 2019 to January 2020.

Results: A total of 46 eligible patients, 26 males, and 20 females were diagnosed with NSCLC in a stage IIIA (30 cases) and IIIB (16 cases) to receive neoadjuvant treatment. The treatment was well tolerated with just 7 (15.2%) typical immune-related adverse events (hyperthyroidism, hyperglycemia, and rash) recorded. A total of 45 patients underwent surgical resection, and 43 (95.6%) of them achieved a R0 resection. No major surgical complications were observed. There was a complete response (CR), partial response (PR), stable disease (SD), and progressive disease (PD) in 2 (4.3%), 26 (56.5%), 17 (37.0%), 6 (26.1%), and 1 (2.2%) patients. Eight patients resulted in a major pathological response (MPR) (17.4%) and 24 patients had a pathological complete response (pCR) (52.2%). At the time of data cutoff (June 1, 2021), the median follow-up period was 15.5 months (IQR 3.9-29) and 27 (60%) of 45 patients who had tumor resection were progression free. At 24 months, progression-free survival was 45.8% and overall survival was 79.9%.

Conclusion: Nivolumab plus paclitaxel and carboplatin could be a potential neoadjuvant regimen for patients with locally advanced NSCLC, rendering a potentially lethal disease to one that is curable.
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http://dx.doi.org/10.2147/CMAR.S344343DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8841324PMC
February 2022

Scaling of the strange-metal scattering in unconventional superconductors.

Nature 2022 02 16;602(7897):431-436. Epub 2022 Feb 16.

Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing, China.

Marked evolution of properties with minute changes in the doping level is a hallmark of the complex chemistry that governs copper oxide superconductivity as manifested in the celebrated superconducting domes and quantum criticality taking place at precise compositions. The strange-metal state, in which the resistivity varies linearly with temperature, has emerged as a central feature in the normal state of copper oxide superconductors. The ubiquity of this behaviour signals an intimate link between the scattering mechanism and superconductivity. However, a clear quantitative picture of the correlation has been lacking. Here we report the observation of precise quantitative scaling laws among the superconducting transition temperature (T), the linear-in-T scattering coefficient (A) and the doping level (x) in electron-doped copper oxide LaCeCuO (LCCO). High-resolution characterization of epitaxial composition-spread films, which encompass the entire overdoped range of LCCO, has enabled us to systematically map its structural and transport properties with unprecedented accuracy and with increments of Δx = 0.0015. We have uncovered the relations T ~ (x - x) ~ (A), where x is the critical doping in which superconductivity disappears and A is the coefficient of the linear resistivity per CuO plane. The striking similarity of the T versus A relation among copper oxides, iron-based and organic superconductors may be an indication of a common mechanism of the strange-metal behaviour and unconventional superconductivity in these systems.
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http://dx.doi.org/10.1038/s41586-021-04305-5DOI Listing
February 2022

The yolk-shell nanorod structure of [email protected] electrodes boosting charge transfer and cyclability in high-performance supercapacitors.

J Colloid Interface Sci 2022 Jun 29;615:133-140. Epub 2022 Jan 29.

College of Chemical and Biological Engineering, Shandong University of Science and Technology, Qingdao 266590, China. Electronic address:

Developing novel electrode materials with reasonable structures and ideal conductivity is of great significance for energy storage devices. In this work, [email protected] yolk-shell nanorods are grown on nickel foam (NF) via a one-step selenization and carbonization process. The carbon shell not only improves the conductivity and charge transfer of electrodes, but also inhibits the dissociation of NiSe core during redox reactions, which is crucial to electrochemical performances of SCs. Owing to the yolk-shell nanorod structure, the [email protected] electrode exhibits an outstanding specific capacitance of 1669.7F g at 1 A g. Moreover, an asymmetric supercapacitor (ASC) is successfully assembled using [email protected] and active carbon (AC) electrodes as the anode and cathode respectively, which delivers remarkable energy-storage characteristics. Specifically, the [email protected]//AC ASC shows a high energy density (31.0 Wh kg) at a power density (723.7 W kg), and stable cycling performance (97% capacitance retention after 9000 cycles). These results make the [email protected] a promising electrode for SCs.
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http://dx.doi.org/10.1016/j.jcis.2022.01.170DOI Listing
June 2022

Manipulating the oxygen reduction reaction pathway on Pt-coordinated motifs.

Nat Commun 2022 Feb 3;13(1):685. Epub 2022 Feb 3.

Institute of Fuel Cells, School of Mechanical Engineering, Shanghai Jiao Tong University, Shanghai, 200240, China.

Electrochemical oxygen reduction could proceed via either 4e-pathway toward maximum chemical-to-electric energy conversion or 2e-pathway toward onsite HO production. Bulk Pt catalysts are known as the best monometallic materials catalyzing O-to-HO conversion, however, controversies on the reduction product selectivity are noted for atomic dispersed Pt catalysts. Here, we prepare a series of carbon supported Pt single atom catalyst with varied neighboring dopants and Pt site densities to investigate the local coordination environment effect on branching oxygen reduction pathway. Manipulation of 2e or 4e reduction pathways is demonstrated through modification of the Pt coordination environment from Pt-C to Pt-N-C and Pt-S-C, giving rise to a controlled HO selectivity from 23.3% to 81.4% and a turnover frequency ratio of HO/HO from 0.30 to 2.67 at 0.4 V versus reversible hydrogen electrode. Energetic analysis suggests both 2e and 4e pathways share a common intermediate of *OOH, Pt-C motif favors its dissociative reduction while Pt-S and Pt-N motifs prefer its direct protonation into HO. By taking the Pt-N-C catalyst as a stereotype, we further demonstrate that the maximum HO selectivity can be manipulated from 70 to 20% with increasing Pt site density, providing hints for regulating the stepwise oxygen reduction in different application scenarios.
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http://dx.doi.org/10.1038/s41467-022-28346-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8813992PMC
February 2022

Fundic gland polyps related to diverse aetiologies show subtle morphological differences: a multicentre retrospective study.

Histopathology 2022 Apr 7;80(5):827-835. Epub 2022 Mar 7.

Department of Pathology, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL, USA.

Aims: Fundic gland polyps (FGPs) comprise 66% of all gastric polyps. Although they are usually non-syndromic, they may be associated with various syndromes, including familial adenomatous polyposis (FAP) or gastric adenocarcinoma and proximal polyposis of the stomach (GAPPS). We aimed to evaluate how histological features relate to distinct FGP subtypes.

Methods And Results: We performed a retrospective analysis of 118 FGPs from 109 patients for the architecture of fundic glands, microcyst lining, parietal cell hyperplasia and surface foveolar epithelial changes. Age, gender and history of FAP or GAPPS were collected. Based on combinations of histological features, three distinct patterns (A, B and C) of FGPs were delineated and correlated to the aetiologies. Non-syndromic FGPs were well-formed polyps composed of disordered fundic glands with intermediate-sized microcysts typically lined by a mixture of oxyntic and mucin-secreting cells (73%). Parietal cell hyperplasia (80%) and foveolar surface hyperplasia (78%) were common. FAP-associated cases demonstrated small microcysts that were predominantly lined by fundic epithelium (77%), with limited parietal cell hyperplasia (27%); foveolar hyperplasia was uncommon. GAPPS-related polyps were the largest, with prominent, mucin-secreting epithelium-lined microcysts (73%). Hyperproliferative aberrant pits were universally present, whereas parietal cell hyperplasia was uncommon. Pattern A was identified in most non-syndromic FGPs (74%) and in a minority of FAP-related FGPs (26%). The majority (82%) of FAP-related FGPs showed pattern B, but only 18% of non-syndromic FGPs did. Pattern C consisted exclusively of GAPPS-associated polyps.

Conclusions: We conclude that, although FGPs share similar histomorphology, subtle differences exist between polyps of different aetiology. In the appropriate clinical setting, the recognition of these variations may help to consider syndromic aetiologies.
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http://dx.doi.org/10.1111/his.14623DOI Listing
April 2022

Identification of inflammatory response and alternative splicing in acute kidney injury and experimental verification of the involvement of RNA‑binding protein RBFOX1 in this disease.

Int J Mol Med 2022 03 21;49(3). Epub 2022 Jan 21.

Department of Urology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China.

An increasing number of inflammatory responses and alternative splicing (AS) have been recently reported to be associated with various kidney diseases. The effect of inflammatory response on acute kidney injury (AKI) has not been fully clarified. In the present study, a mouse model of AKI induced by cisplatin and ischemia‑reperfusion (IR) was established and genome‑wide profiling analysis and identification of differentially expressed genes (DEGs) in kidney tissue was conducted by Gene Ontology (GO) functional analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis, protein‑protein interaction (PPI) network analysis and RT‑qPCR. The results revealed that common DEGs in AKI induced by cisplatin and IR were enriched in the inflammatory response pathway, including hub genes CSF‑1, CXCL1, CXCL10, IL‑1β, IL‑34, IL‑6 and TLR2. AS in AKI was initially reported. Cisplatin‑induced AS was enriched in the phosphorylation pathway, involving regulated AS genes CSNK1A1, PAK2, CRK, ADK and IKBKB. IR‑induced AS was enriched in apoptosis and proliferation pathways, including DEGs ZDHHC16, BCL2L1 and FGF1 regulated by AS. The ability of RNA‑binding proteins (RBPs) to regulate AS was coordinated with the function of context‑dependent genetic mechanisms. A total of 49 common differentially expressed RBP genes were screened. RNA binding fox‑1 homolog 1 (RBFOX1) was revealed to be the top downregulated gene. The relative levels of RBFOX1 in the nuclei of mouse renal tubular epithelial cells in mRNA and proteins were downregulated by cisplatin and IR. Moreover, the biological functions of RBFOX1 were investigated in human renal proximal tubular epithelial cells (HK‑2 cells). Results of experiments revealed that exogenous RBFOX1 inhibited inflammation and oxidative stress to reduce hypoxia/reoxygenation‑induced apoptosis of HK‑2 cells. This phenomenon may be related to the inhibition of NF‑κB and the activation of the NRF2/HO‑1 signaling pathway. In conclusion, the inflammatory cytokines, AS and RBPs in AKI were analyzed in the present study via whole transcriptome sequencing. It was revealed that the RBP gene RBFOX1 was involved in the pathogenesis of AKI. Thus, the present study provided novel insights into the mechanism of AKI pathogenesis.
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http://dx.doi.org/10.3892/ijmm.2022.5087DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8788925PMC
March 2022

Highly galloylated and A-type prodelphinidins and procyanidins in persimmon (Diospyros kaki L.) peel.

Food Chem 2022 Jun 29;378:131972. Epub 2021 Dec 29.

College of Food Science and Engineering, Northwest A & F University, Yangling 712100, China. Electronic address:

The structure of persimmon peel proanthocyanidins (PPPAs) was characterized. After acid catalysis of PPPAs in the presence of phloroglucinol, five reaction products such as (epi)gallocatechin-phloroglucinol ((E)GC-ph) etc. were identified by HPLC-DAD-ESI/MS. FT-IR analysis confirmed that all of the compounds exhibited a 2, 3-cis configuration. Therefore, the extension units in PPPAs were EGC, epigallocatechin gallate (EGCG), epicatechin, and epicatechin gallate and only EGCG was detected as the terminal unit. PPPAs contained 25.21% of procyanidins and 74.79% of prodelphindins and had a high degree of 3-O-galloylation (>74.79%). The mean degree of polymerization of PPPAs was calculated to be 10.18. MALDI-TOF MS analysis showed that A-type linkage and galloylation existed commonly in PPPAs.
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http://dx.doi.org/10.1016/j.foodchem.2021.131972DOI Listing
June 2022

Electronic nature of charge density wave and electron-phonon coupling in kagome superconductor KVSb.

Nat Commun 2022 Jan 12;13(1):273. Epub 2022 Jan 12.

Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, 100190, Beijing, China.

The Kagome superconductors AVSb (A = K, Rb, Cs) have received enormous attention due to their nontrivial topological electronic structure, anomalous physical properties and superconductivity. Unconventional charge density wave (CDW) has been detected in AVSb. High-precision electronic structure determination is essential to understand its origin. Here we unveil electronic nature of the CDW phase in our high-resolution angle-resolved photoemission measurements on KVSb. We have observed CDW-induced Fermi surface reconstruction and the associated band folding. The CDW-induced band splitting and the associated gap opening have been revealed at the boundary of the pristine and reconstructed Brillouin zones. The Fermi surface- and momentum-dependent CDW gap is measured and the strongly anisotropic CDW gap is observed for all the V-derived Fermi surface. In particular, we have observed signatures of the electron-phonon coupling in KVSb. These results provide key insights in understanding the nature of the CDW state and its interplay with superconductivity in AVSb superconductors.
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http://dx.doi.org/10.1038/s41467-021-27946-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8755796PMC
January 2022

Monoterpene indole alkaloids from Melodinus cochinchinensis (Lour.) Merr.

Fitoterapia 2022 Mar 31;157:105118. Epub 2021 Dec 31.

Natural Product Research Center, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China; School of Pharmacy, University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address:

Melodicochinines A - D (1-4), four new monoterpene indole alkaloids (MIAs), along with 21 known ones, were isolated from the stems and twigs of Melodinus cochinchinensis. Their structures were elucidated on the basis of extensive spectroscopic analysis. A ubiquitin-rhodamine 110 assay showed that 11-methyloxytabersonine had potential inhibitory effect against ubiquitin-specific protease 7 (USP7).
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http://dx.doi.org/10.1016/j.fitote.2021.105118DOI Listing
March 2022
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