Publications by authors named "Kui Zhao"

285 Publications

Fabrication And In Vitro Evaluation Of 3D Printed Porous Silicate Substituted Calcium Phosphate Scaffolds For Bone Tissue Engineering.

Biotechnol Bioeng 2022 Aug 3. Epub 2022 Aug 3.

Department of Orthopaedic Surgery, The Second Affiliated Hospital of Harbin Medical University, Xuefu Road 246, Harbin, Heilongjiang, 150081, P. R. China.

Silicate-substituted calcium phosphate (Si-CaP) ceramics, alternative materials for autogenous bone grafting, exhibit excellent osteoinductivity, osteoconductivity, biocompatibility and biodegradability; thus, they have been widely used for treating bone defects. However, the limited control over the spatial structure and weak mechanical properties of conventional Si-CaP ceramics hinder their wide application. Here, we used digital light processing (DLP) printing technology to fabricate a novel porous 3D printed Si-CaP scaffold to enhance the scaffold properties. Scanning electron microscopy, compression tests, and computational fluid dynamics simulations of the 3D printed Si-CaP scaffolds revealed a uniform spatial structure, appropriate mechanical properties, and effective interior permeability. Furthermore, compared to Si-CaP groups, 3D printed Si-CaP groups exhibited sustained release of silicon (Si), calcium (Ca) and phosphorus (P) ions. Furthermore, 3D printed Si-CaP groups had more comprehensive and persistent osteogenic effects due to increased osteogenic factor expression and calcium deposition. Our results show that the 3D printed Si-CaP scaffold successfully improved bone marrow mesenchymal stem cell (BMSCs) adhesion, proliferation and osteogenic differentiation and possessed a distinct apatite mineralization ability. Overall, with the help of DLP printing technology, Si-CaP ceramic materials facilitate the fabrication of ideal bone tissue engineering scaffolds with essential elements, providing a promising approach for bone regeneration. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/bit.28202DOI Listing
August 2022

Clinical features and F-FDG PET/CT for distinguishing of malignant lymphoma from inflammatory lymphadenopathy in HIV-infected patients.

BMC Infect Dis 2022 Jul 27;22(1):646. Epub 2022 Jul 27.

State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310003, China.

Background: It is vital to distinguish between inflammatory and malignant lymphadenopathy in human immunodeficiency virus (HIV) infected individuals. The purpose of our study was to differentiate the variations in the clinical characteristics of HIV patients, and apply F-FDG PET/CT parameters for distinguishing of malignant lymphoma and inflammatory lymphadenopathy in such patients.

Methods: This retrospective cross-sectional study included 59 consecutive HIV-infected patients who underwent whole-body F-FDG PET/CT. Of these patients, 37 had biopsy-proven HIV-associated lymphoma, and 22 with HIV-associated inflammatory lymphadenopathy were used as controls. The determined parameters were the maximum of standard uptake value (SUV), SUV of only lymph nodes (SUV), the most FDG-avid lesion-to-liver SUV ratio (SUR), laboratory examinations and demographics. The optimal cut-off of F-FDG PET/CT value was analyzed by receiver operating characteristic curve (ROC).

Results: Considering the clinical records, the Karnofsky Performance Status (KPS) scores in patients with inflammatory lymphadenopathy were obviously higher than those in patients with malignant lymphoma (P = 0.015), whereas lymphocyte counts and lactate dehydrogenase (LDH) were obviously lower (P = 0.014 and 0.010, respectively). For the F-FDG PET/CT imaging, extra-lymphatic lesions, especially digestive tract and Waldeyer's ring, occurred more frequently in malignant lymphoma than inflammatory lymphadenopathy. Furthermore, the SUR and SUV in malignant lymphoma were markedly higher than those in inflammatory lymphadenopathy (P = 0.000 and 0.000, respectively). The cut-off point of 3.1 for SUR had higher specificity (91.9%) and relatively reasonable sensitivity (68.2%) and the cut-off point of 8.0 for the SUV had high specificity (89.2%) and relatively reasonable sensitivity (63.6%).

Conclusion: Our study identified the distinctive characteristics of the clinical manifestations, the SUR, SUV and detectability of extra-lymphatic lesions on F-FDG PET, and thus provides a new basis for distinguishing of malignant lymphoma from inflammatory lymphadenopathy in HIV-infected patients.
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http://dx.doi.org/10.1186/s12879-022-07640-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9327211PMC
July 2022

Synchronous Surface Reconstruction and Defect Passivation for High-Performance Inorganic Perovskite Solar Cells.

Small 2022 Jul 20:e2202690. Epub 2022 Jul 20.

Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, Shaanxi Key Laboratory for Advanced Energy Devices, Shaanxi Engineering Lab for Advanced Energy Technology, School of Materials Science and Engineering, Shaanxi Normal University, No. 620, West Chang'an Avenue, Xi'an, 710119, P. R. China.

The nonradiative charge recombination caused by surface defects and inferior crystalline quality are major roadblocks to further enhancing the performance of CsPbI Br perovskite solar cells (PSCs). Theoretical calculations indicate that sodium diethyldithiocarbamate (NaDDTC), a popular bacteriostatic benign material, can initiate multiple interactions with the CsPbI Br perovskite surface to effectively passivate the defects. The experimental results reveal that the NaDDTC can indeed passivate the electron trap states and lock active sites for charge traps and water adsorption. In addition, it is found that a solid-state reaction is triggered for perovskite crystal regrowth by the NaDDTC post-treatment, which not only enlarges grain size for reducing the density of grain boundary defects but also compensates some surface defects induced by the primary film growth. Consequently, the power conversion efficiency (PCE) of the CsPbI Br PSC is increased to as high as 20.40%, with significant improvement in fill factor and open-circuit voltage (V ), making it one of the highest for this type of solar cell. Furthermore, the optimized devices exhibit better environmental stability. Overall, this robust synchronous strategy provides efficient surface reconstruction and defect passivation for achieving both high PCE and stable inorganic perovskite.
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http://dx.doi.org/10.1002/smll.202202690DOI Listing
July 2022

Roles of Organic Ligands in Ambient Stability of Layered Halide Perovskites.

ACS Appl Mater Interfaces 2022 Jul 13. Epub 2022 Jul 13.

Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education; Shaanxi Key Laboratory for Advanced Energy Devices; Shaanxi Engineering Lab for Advanced Energy Technology, School of Materials Science and Engineering, Shaanxi Normal University, Xi'an 710119, China.

The combination of organic ligands and inorganic Pb-I frameworks in layered perovskites has bestowed upon them high structural tunability and stability, while their microscopic degradation mechanism remains unclear. Here, we found the key role of ligands in intrinsic structural stability and the consequent morphological evolution in layered perovskites during long-term ambient aging based on (GA)(MA)PbI (GA = guanidinium, <> = 4) and (BDA)(MA)PbI (BDA = 1,4-butanediammonium, < > = 4) perovskites. The BDA-based perovskites have a low intrinsic stability due to high crystal formation energy (Δ), which are prone to hydration during ambient aging. We overserved changed crystal orientation from perpendicular to parallel, a delayed charge populating time from <1 ps to >50 ps, an inhibited carrier transfer kinetics between quantum wells, an increase of 0.9 μs of charge carrier transport time and a decrease of 1.2 μs of charge carrier lifetime in the BDA-based film during ambient aging, which accounts for a large power-conversion efficiency (PCE) loss (14.2% vs 11.2%). By contrast, the GA ligand increases the intrinsic structural stability of perovskites, which not only yields an initial PCE as high as 20.0% but also helps retain excellent optoelectronic properties during aging. Therefore, only a slight PCE loss (20.0% vs 19.1%) was observed. Our work reveals the key role of organic-inorganic interaction affecting the intrinsic structural stability and optoelectronic properties, and provides a theoretical basis for the future design of stable and efficient optoelectronic devices.
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http://dx.doi.org/10.1021/acsami.2c05348DOI Listing
July 2022

Controlling Phase Transition toward Future Low-Cost and Eco-friendly Printing of Perovskite Solar Cells.

J Phys Chem Lett 2022 Jul 12;13(28):6503-6513. Epub 2022 Jul 12.

Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, Shaanxi Key Laboratory for Advanced Energy Devices, Shaanxi Engineering Lab for Advanced Energy Technology, School of Materials Science and Engineering, Shaanxi Normal University, Xi'an 710119, Shaanxi, China.

Perovskite solar cells (PSCs) have grown increasingly popular over the past few years and are considered to be game-changers in the energy conversion market. It has became vital to transfer the deep understanding of the perovskite film formation process during lab-scale fabrication to large-scale production. Complex phase transition during film formation has been revealed by strategies. However, there is still debate which phase transition is the right route for a future scalable approach. Herein, we briefly summarize perovskite crystallization during scalable printing processes. The critical information about the intermediates involved in phase transition from precursors to perovskite crystals are discussed because it deeply impacts the morphology of printed films. Finally, important strategies to control phase transition and challenges toward future low-temperature and eco-friendly printing of perovskite solar cells are proposed. The information provided by this Perspective will assist the screening and development of the perovskite phase transition for future cost-efficient printed perovskite panels.
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http://dx.doi.org/10.1021/acs.jpclett.2c01506DOI Listing
July 2022

Efficient Eco-Friendly Flexible X-ray Detectors Based on Molecular Perovskite.

Nano Lett 2022 07 12;22(14):5973-5981. Epub 2022 Jul 12.

Key Laboratory of Applied Surface and Colloid Chemistry, National Ministry of Education; Shaanxi Key Laboratory for Advanced Energy Devices; Shaanxi Engineering Lab for Advanced Energy Technology; Institute for Advanced Energy Materials; School of Materials Science and Engineering, Shaanxi Normal University, Xi'an 710119, People's Republic of China.

Next-generation wearable electronics requires mechanical robustness. In addition to the previously reported eco-friendliness, low cost, and light weight of molecular perovskites, flexibility is also a desired merit for their practical use. Here we design a flexible X-ray detector based on a novel molecular perovskite, DABCO-CsBr (DABCO = -'-diazabicyclo[2.2.2]octonium), which is the missing link between metal-free molecular perovskites A(NH)X (A = divalent organic ammoniums) and conventional metal halide based ABX (B = divalent metal cations) perovskites. DABCO-CsBr inherits its band nature from A(NH)X, while it exhibits a stronger stopping power. DABCO-CsBr shows potential for high-performance ionizing radiation detectors due to low dark current, low ion migration, and an efficient mobility-lifetime (μτ) product. Finally, a molecular-perovskite-based flexible X-ray detector is demonstrated on the basis of the DABCO-CsBr/poly(vinylidene fluoride) composite, with a sensitivity of 106.7 μC Gy cm. This work enriches the molecular perovskite family and highlights the promise of molecular perovskites for the next-generation eco-friendly and wearable optoelectronic devices.
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http://dx.doi.org/10.1021/acs.nanolett.2c02071DOI Listing
July 2022

Epidemiological Investigation of Porcine Pseudorabies Virus in Hebei Province, China, 2017-2018.

Front Vet Sci 2022 24;9:930871. Epub 2022 Jun 24.

College of Veterinary Medicine, Hebei Agricultural University, Baoding, China.

Pseudorabies (PR) is a serious disease affecting the pig industry in China, and it is very important to understand the epidemiology of pseudorabies virus (PRV). In the present study, 693 clinical samples were collected from Bartha-K61 vaccinated pigs with symptoms of suspected PRV infection between January 2017 and December 2018. All cases were referred for full clinical autopsy with detailed examination of histopathological examination, virus isolation and genetic evolution analysis of the PRV glycoprotein E () gene. In addition, PRV antibodies in 3,449 serum samples were detected by the enzyme-linked immunosorbent assay (ELISA). The clinical data revealed that abortion and stillbirth are the most frequent appearances in pregnant sows of those cases. Histopathological examination exhibited a variety of pathological lesions, such as lobar pneumonia, hepatitis, lymphadenitis, nephritis, and typical nonsuppurative encephalitis. A total of 248 cases tested positive for the PRV gE gene. 11 PRV variants were isolated and confirmed by gene sequencing and phylogenetic analysis. These strains had 97.1%-100.0% nucleotide homology with the PRV reference strains. Notably, the isolated strains were highly homologous and clustered in the same branch as HSD-1/2019, which caused human acute encephalitis. Serological tests showed that the positive rate of PRV antibody in the 3449 serum samples collected from the Hebei Province was 46.27%. In conclusion, PRV variant strains Are high prevalence in the Hebei Province, which not only causes huge economic losses to the breeding industry but also potentially poses a threat to public health.
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http://dx.doi.org/10.3389/fvets.2022.930871DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9263846PMC
June 2022

Complete genomic sequences and comparative analysis of two Orf virus isolates from Guizhou Province and Jilin Province, China.

Virus Genes 2022 Jul 3. Epub 2022 Jul 3.

Key Laboratory of Zoonosis Research, College of Veterinary Medicine, Jilin University, Ministry of Education, Changchun, China.

Orf virus (ORFV, species Orf virus) belongs to the typical species of the Parapoxvirus genus of the family Poxviridae, which infects sheep, goats, and humans with worldwide distribution. Although outbreaks of Orf have been reported sequentially in several Chinese provinces, the epidemiology of Orf and genetic diversity of ORFV strains still needs to be further characterized. To further reveal the genomic organization of the ORFV-GZ18 and ORFV-CL18 isolates, the complete genome sequences of two recently obtained ORFV isolates were sequenced using the next-generation sequencing technology and analyzed, which had been deposited in the GenBank database under accession number MN648218 and MN648219, respectively. The complete genomic sequence of ORFV-CL18 was 138,495 bp in length, including 131 potential open reading frames (ORFs) flanked by inverted terminal repeats (ITRs) of 3481 bp at both ends, which has genomic structure typical Parapoxviruses. The overall genomic organization of the fully sequenced genome of ORFV-GZ18 was consistent with ORFV-CL18 genome, with a complete genome size of 138,446 nucleotides, containing 131 ORFs flanked by ITRs of 3469 bp. Additionally, the overall G + C contents of ORFV-GZ18 and ORFV-CL18 genome sequences were about 63.9% and 63.8%, respectively. The phylogenetic analysis showed that both ORFV-GZ18 and ORFV-CL18 were genetically closely related to ORFV-SY17 derived from sheep. In summary, the complete genomic sequences of ORFV-GZ18 and ORFV-CL18 are reported, with the hope it will be useful to investigate the host range, geographic distribution, and genetic evolution of the virus in Southern West and Northern East China.
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http://dx.doi.org/10.1007/s11262-022-01918-4DOI Listing
July 2022

PHEV infection: A promising model of betacoronavirus-associated neurological and olfactory dysfunction.

PLoS Pathog 2022 06 27;18(6):e1010667. Epub 2022 Jun 27.

State Key Laboratory for Zoonotic Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China.

Porcine hemagglutinating encephalomyelitis virus (PHEV) is a highly neurotropic coronavirus belonging to the genus Betacoronavirus. Similar to pathogenic coronaviruses to which humans are susceptible, such as SARS-CoV-2, PHEV is transmitted primarily through respiratory droplets and close contact, entering the central nervous system (CNS) from the peripheral nerves at the site of initial infection. However, the neuroinvasion route of PHEV are poorly understood. Here, we found that BALB/c mice are susceptible to intranasal PHEV infection and showed distinct neurological manifestations. The behavioral study and histopathological examination revealed that PHEV attacks neurons in the CNS and causes significant smell and taste dysfunction in mice. By tracking neuroinvasion, we identified that PHEV invades the CNS via the olfactory nerve and trigeminal nerve located in the nasal cavity, and olfactory sensory neurons (OSNs) were susceptible to viral infection. Immunofluorescence staining and ultrastructural observations revealed that viral materials traveling along axons, suggesting axonal transport may engage in rapid viral transmission in the CNS. Moreover, viral replication in the olfactory system and CNS is associated with inflammatory and immune responses, tissue disorganization and dysfunction. Overall, we proposed that PHEV may serve as a potential prototype for elucidating the pathogenesis of coronavirus-associated neurological complications and olfactory and taste disorders.
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http://dx.doi.org/10.1371/journal.ppat.1010667DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9282652PMC
June 2022

The Distribution Characteristics of Aerosol Bacteria in Different Types of Pig Houses.

Animals (Basel) 2022 Jun 14;12(12). Epub 2022 Jun 14.

Changchun Veterinary Research Institute, Chinese Academy of Agriculture Sciences, Changchun 130122, China.

With the development of modern pig raising technology, the increasing density of animals in pig houses leads to the accumulation of microbial aerosols in pig houses. It is an important prerequisite to grasp the characteristics of bacteria in aerosols in different pig houses to solve the problems of air pollution and disease prevention and control in different pig houses. This work investigated the effects of growth stages on bacterial aerosol concentrations and bacterial communities in pig houses. Three traditional types of closed pig houses were studied: farrowing (FAR) houses, weaning (WEA) houses, and fattening (FAT) houses. The Andersen six-stage sampler and high-volume air sampler were used to assess the concentrations and size distribution of airborne bacteria, and 16S rRNA gene sequencing was used to identify the bacterial communities. We found that the airborne bacterial concentration, community richness, and diversity index increased with pig age. We found that , , , , and in the microbial aerosols of pig houses have the potential risk of causing disease. These differences lead us to believe that disinfection strategies for pig houses should involve a situational focus on environmental aerosol composition on a case-by-case basis.
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http://dx.doi.org/10.3390/ani12121540DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9219456PMC
June 2022

Anti-Influenza Effect and Mechanisms of Lentinan in an ICR Mouse Model.

Front Cell Infect Microbiol 2022 20;12:892864. Epub 2022 May 20.

Changchun Veterinary Research Institute, Chinese Academy of Agriculture Sciences, Changchun, China.

Influenza virus is a serious threat to global human health and public health security. There is an urgent need to develop new anti-influenza drugs. Lentinan (LNT) has attracted increasing attention in recent years. As potential protective agent, LNT has been shown to have anti-tumor, anti-inflammatory, and antiviral properties. However, there has been no further research into the anti-influenza action of lentinan , and the mechanism is still not fully understood. In this study, the anti-influenza effect and mechanism of Lentinan were studied in the Institute of Cancer Research (ICR) mouse model. The results showed that Lentinan had a high degree of protection in mice against infection with influenza A virus, delayed the emergence of clinical manifestations, improved the survival rate of mice, significantly prolonged the middle survival days, attenuated the weight loss, and reduced the lung coefficient of mice. It alleviated the pathological damage of mice infected with the influenza virus and improved blood indices. Lentinan treatment considerably inhibited inflammatory cytokine (TNF-α, IL-1β, IL-4, IL-5, IL-6) levels in the serum and lung and improved IFN-γ cytokine levels, which reduced cytokine storms caused by influenza virus infection. The underlying mechanisms of action involved Lentinan inhibiting the inflammatory response by regulating the TLR4/MyD88 signaling pathway. This study provides a foundation for the clinical application of Lentinan, and provides new insight into the development of novel immunomodulators.
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http://dx.doi.org/10.3389/fcimb.2022.892864DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9163413PMC
May 2022

Nonocclusive mesenteric ischemia caused by type B aortic dissection: a case report.

BMC Surg 2022 Jun 3;22(1):214. Epub 2022 Jun 3.

Second Affiliated Hospital of Soochow University, Suzhou, China.

Background: Nonocclusive mesenteric ischemia (NOMI) is defined as acute intestinal ischemia because of decreased blood flow in mesenteric vessels. Only a few cases of NOMI that occur secondary to aortic dissection (AD) have been reported, resulting in the lack of sufficient knowledge of diagnosis and treatment.

Case Presentation: We aimed to report a case of NOMI caused by type B Aortic Dissection. A 26-year-old male patient was transferred to our hospital with the diagnose of NOMI and aortic dissection in April 2018. The abdominal computed tomography (CT) assists the diagnosis of paralytic intestinal obstruction, intestinal wall pneumatosis, and perforation. Emergency laparotomy revealed that the bowel wall supplied by the superior mesenteric artery (SMA) was pale with the palpable but weak pulsation of the parietal artery. The small intestine was extremely dilated with a paper-thin, fragile wall that was ruptured easily and could not be sutured. In this case, extensive resection and segmental drainage were done. Postoperatively, the digestive tract was reconstructed. However, the patient suffered from iron deficiency anemia and short bowel syndrome eight months later, and unfortunately died from long-term complications.

Conclusion: Aortic dissection leads to continuous decrease in blood pressure and blood flow to the SMA, considering as a predisposing factor for NOMI. During the treatment, extensive resection and segmental drainage are the optimal surgical strategy, which can make benefit in emergencies especially.
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http://dx.doi.org/10.1186/s12893-022-01656-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9166441PMC
June 2022

Structural and Functional Insights into Metal-Free Perovskites.

J Phys Chem Lett 2022 Jun 5:5168-5178. Epub 2022 Jun 5.

Key Laboratory of Applied Surface and Colloid Chemistry, National Ministry of Education; Shaanxi Key Laboratory for Advanced Energy Devices; Shaanxi Engineering Lab for Advanced Energy Technology; Institute for Advanced Energy Materials; School of Materials Science and Engineering, Shaanxi Normal University, Xi'an 710119, P.R. China.

In the past three years, metal-free perovskites have garnered significant interest as promising candidates for utilization in next-generation wearable electronics. A variety of different molecular structures for these perovskites have been designed for different applications. However, there is still no systematic understanding that can elucidate the relationship between the structural details and properties of perovskites. This would provide a helpful guide for designing a metal-free perovskite with the desired packing structure and properties. Herein, we summarize recently reported structural and functional insights into metal-free perovskites. The underlying design of the molecular structure and its role in the packing structure and resulting properties are explained. In addition, important factors and challenges in the design of a molecular structure that will be useful for future applications are discussed. This information will help enrich the library of potential structures and future applications of metal-free perovskites, which is believed to be much larger than is currently known.
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http://dx.doi.org/10.1021/acs.jpclett.2c01044DOI Listing
June 2022

Processing of Lead Halide Perovskite Thin Films Studied with In-Situ Real-Time X-ray Scattering.

ACS Appl Mater Interfaces 2022 May 31. Epub 2022 May 31.

Center for Advanced Microelectronics Manufacturing and Materials Science and Engineering Program, Binghamton University, Binghamton, New York 13902, United States.

Lead halide perovskites have been of paramount interest for solution-processable solar cells, reaching power conversion efficiencies larger than 25%. In this spotlight, we will provide a systematic overview of the influence of different solution-based processing routes of lead halide perovskites on their phase transformation and conversion as revealed through in-situ X-ray-scattering experiments. These experiments were performed in conditions closely mimicking thin film processing methods and conditions used for thin film solar cell device fabrication and therefore provide critical information about the mechanism of the phase transformation, its onset, the kinetics, as well as the emergence and disappearance of various (meso)phases along the way. The measurements capture the overall solidification and conversion process of lead halide perovskite inks into solid films via so-called one-step and two-step spin-coating processes as well as blade coating and hot casting. Processing routes are applied to films based on basic components as well as mixtures of different anions and cations, solvents, and antisolvents, all of which deeply affect the thin film microstructure and morphology of the light-absorbing semiconductor and associated solar cell devices.
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http://dx.doi.org/10.1021/acsami.2c03153DOI Listing
May 2022

Prognostic value of node-to-primary tumor maximum standardized uptake value ratio in T1-4N1-3M0 non-small cell lung cancer patients treated with concurrent chemo-radiotherapy.

Nucl Med Commun 2022 Aug 13;43(8):901-907. Epub 2022 May 13.

Departments of PET Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, China.

Background: This study aimed to identify whether NTR is the independent risk factor for progression-free survival (PFS) and overall survival (OS) in patients treated with concurrent chemo-radiotherapy (cCRT).

Methods: We retrospectively studied 106 T1-4N1-3M0 non-small cell lung cancer patients treated with cCRT. The maximum standardized uptake value (SUVTumor) of the primary tumor and the metastatic lymph nodes (SUVLN) were measured. The prognostic significance of NTR for predicting PFS and OS was assessed. A multi-adjusted spline regression model was conducted to provide more precise estimates and examine the shape of the associations between NTR and the risk of progression.

Results: From 2012 to 2017, 106 eligible patients were analyzed. The median follow-up time was 15.3 months (3.5-44.6 months). We determined the maximizing area under the time-dependent receiver operating characteristic curve was at an NTR of 0.73 for predicting PFS. The two-year PFS was significantly lower in the high-NTR group (35.7% vs. 55.4%, P = 0.02) and two-year OS (43.4% vs. 61.1%, P = 0.03 was also significantly worse. Multivariable analysis revealed that only NTR was an independent prognostic factor for PFS (hazard ratio [HR]: 10.04, P < 0.001) and OS (HR: 4.19, P = 0.03). The restricted cubic spline regression model showed that NTR had a non-linear relationship with log relative risk for progression.

Conclusion: NTR was an independent risk factor for predicting PFS and OS in T1-4N1-3M0 non-small cell lung cancer patients treated with cCRT.
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http://dx.doi.org/10.1097/MNM.0000000000001576DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9278701PMC
August 2022

THUMPD3-AS1 facilitates cell growth and aggressiveness by the miR-218-5p/SKAP1 axis in colorectal cancer.

Cell Biochem Biophys 2022 May 10. Epub 2022 May 10.

Department of General Surgery, The Second Affiliated Hospital of Soochow University, Suzhou, 215004, Jiangsu, China.

Background: Colorectal cancer (CRC) is a malignant cancer with a high mortality. Accumulating studies have revealed that mRNAs involved in ceRNA (competing endogenous RNA) network are implicated in the tumorigenesis and development of CRC. Here, we aimed to elucidate the ceRNA network involving Src kinase associated phosphoprotein 1 (SKAP1) in the biological characteristics of CRC.

Methods: Expression levels of genes in colon adenocarcinoma (COAD) samples and prognosis of COAD patients were predicted using publicly available online tool. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), clony formation and Transwell assays were conducted to test the biological functions of SKAP1 and THUMPD3 antisense RNA 1 (THUMPD3-AS1) in CRC cells. Western blot was used to measure the protein levels of SKAP1. Gene expression in CRC cells was detected by reverse transcription quantitative polymerase chain reaction (RT-qPCR). The interaction between miR-218-5p and THUMPD3-AS1 (or SKAP1) was verified by RNA pulldown and luciferase reporter assays.

Results: SKAP1 was upregulated in COAD tissues and CRC cells and it reflected a poor prognosis in patients with COAD. SKAP1 knockdown inhibited CRC (HT-29 and HCT-116) cell proliferation, migration and invasion. Mechanistically, THUMPD3-AS1 acted as a ceRNA to sponge miR-218-5p and subsequently upregulated SKAP1 expression in CRC cells. SKAP1 overexpression reversed the suppressive effect of THUMPD3-AS1 knockdown on proliferation, migration and invision of CRC cells.

Conclusions: THUMPD3-AS1 promotes CRC cell growth and aggressiveness by regulating the miR-218-5p/SKAP1 axis.
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http://dx.doi.org/10.1007/s12013-022-01074-4DOI Listing
May 2022

Microtubule depolymerization limits porcine betacoronavirus PHEV replication.

Vet Microbiol 2022 Jun 27;269:109448. Epub 2022 Apr 27.

Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China; Key Lab of Animal Production, Product Quality and Security, Ministry of Education, Jilin Agricultural University, Changchun, China. Electronic address:

Porcine hemagglutinating encephalomyelitis virus (PHEV) is a typical neurotropic betacoronavirus causing digestive disease and/or neurological dysfunction in neonatal pigs. Actin filaments have been identified to implicate in PHEV invasion, but the effects of viral infection on microtubules (MTs) cytoskeleton are unknown. Here, we observed that PHEV infection induced MT depolymerization and was accompanied by the disappearance of microtubule organizing centers. Depolymerization of MTs induced by nocodazole significantly inhibited viral RNA replication, but over-polymerization of MTs induced by paclitaxel did not substantially affect PHEV infection. The expression of histone deacetylase 6 (HDAC6), an important regulator of MT acetylation, progressively increased during PHEV infection. Tramstatin A could alter HDAC6 deacetylase activity to enhance the acetylation of the substrate α-tubulin and MT polymerization, but does not increase PHEV proliferation. These findings suggest that PHEV could subvert host MT cytoskeleton to facilitate infection, and that MT depolymerization negatively affects viral replication independently of HDAC6 activity.
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http://dx.doi.org/10.1016/j.vetmic.2022.109448DOI Listing
June 2022

Effects of Nano-CuO on the Productivity in the Cu-Stripped Chinese Merino Sheep.

Biol Trace Elem Res 2022 May 4. Epub 2022 May 4.

Animal Husbandry and Veterinary Research Institute, Guizhou Province Academy of Agricultural Sciences, Guiyang, 550005, China.

This research is to study the effects of nano-CuO on blood parameters, growth performance, and wool yield in the Cu-stripped ranches, and provide reference for further study on prevention and treatment in Cu-deficient Chinese Merino sheep. We selected twenty Chinese Merino sheep from Cu-deficient and normal ranch, respectively. The results have shown that the Cu content in the soils, forages, blood, and wool from Cu-deficient ranches was significantly lower than those from normal ranches (P < 0.01). There were no significant differences in the contents of other elements. Treatment experiments of nano-CuO were carried out for 50 days. We found that the Cu content in the blood was increased continuously in the sheep after adding nano-CuO, and reached the highest level at 40 days. The levels of Hb, RBC, PCV, MCV, and MCH in the nano-CuO-fed animals were remarkably higher than those in the control animals (P < 0.01). The daily gain and wool yield in the nano-CuO-fed animals were also significantly higher than those in the control sheep (P < 0.01). Therefore, adding nano-CuO not only remarkably increases the Cu content in the blood, but also greatly improves the daily gain and wool yield in Cu-deficient animals.
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http://dx.doi.org/10.1007/s12011-022-03245-zDOI Listing
May 2022

Molybdenum fertilizer improved antioxidant capacity of Chinese Merino sheep under compound contamination.

Biol Trace Elem Res 2022 May 4. Epub 2022 May 4.

School of Materials and Architectural Engineering, Guizhou Normal University, Guiyang, 550025, China.

To investigate the response of different levels of molybdenum (Mo) fertilizer to Chinese Merino sheep (Junken Type) grazing on natural heavy metal-contaminated meadows, this study was carried out in the Bayanbulak Grassland lying in the northwest of Xinjiang Uygur Autonomous Region, China. A total of 24-hm polluted meadows were fenced and were randomly divided into four groups (3 replication/group and 2 hm/replication) applied 0-kg Mo, 1-kg Mo, 2-kg Mo, and 3-kg Mo (ammonium molybdate tetrahydrate) per hectare for the CON group, group I, group II, and group III, respectively. Seventy-two healthy 1-year-old Chinese Merino sheep (45.56 ± 2.35 kg) were randomly assigned to the tested pastures for 90 days. Compared with the CON group, the Mo content from fertilized groups and the Se content from group II and group III in serums and livers were significantly increased (P < 0.05), and the Cu content from fertilized groups in serums and livers was significantly decreased (P < 0.05). The levels of blood Hb and RBC, and the activities of serum SOD, CAT, GSH-Px, and Cp in group III, were significantly higher (P < 0.05) than those in the CON group, group I, and group II. Serum MDA content in group III was significantly lower (P < 0.05) than that in the other three groups. In summary, Mo fertilization improved the antioxidant capacity of grazing sheep and also reduced the toxic damage to Chinese Merino sheep grazing on natural grasslands contaminated by heavy metals, but Mo poisoning caused by excessive fertilization should be prevented.
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http://dx.doi.org/10.1007/s12011-022-03266-8DOI Listing
May 2022

Prognostic usefulness of clinical features and pretreatment F-FDG PET/CT metabolic parameters in patients with angiosarcoma.

Quant Imaging Med Surg 2022 May;12(5):2792-2804

PET Center, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Background: To investigate the prognostic value of clinical features and metabolic parameters in pretreatment F-2-fluoro-2-deoxy-D-glucose (F-FDG) positron emission tomography/X-ray computed tomography (PET/CT) scans of patients with angiosarcoma, a rare neoplasm that has not been well characterized.

Methods: In this retrospective study, 19 patients with a histopathologically confirmed diagnosis of angiosarcoma who had undergone pretreatment F-FDG PET/CT scans were enrolled. We recorded the age at presentation, sex, underlying diseases, sites of primary tumors, Karnofsky Performance Status (KPS) score, Eastern Cooperative Oncology Group (ECOG) score, time from onset to diagnosis, laboratory examinations, sites and sizes of primary tumors, treatment modalities, histologic features and American Joint Committee on Cancer (AJCC) stage, maximum standardized uptake value (SUVmax), average SUV (SUVavg), metabolic tumor volume (MTV), and total lesion glycolysis (TLG) of primary tumors and the whole body. Univariate and multivariate survival analyses for overall survival were performed according to the metabolic parameters and other clinical variables.

Results: Patients ranged in age from 27 to 79 years (median: 59 years) with different angiosarcoma types covering all tumor grades and subtypes. Seven (7/19) patients had anemia of varying degrees of severity. Lymph node metastases (n=10) and/or distant metastases (n=11) of angiosarcoma were common. Bone or bone marrow (10/19) and lung (8/19) were the most common distant metastatic organs. Patients with bone metastases, low hemoglobin levels and high ferritin levels had significantly poorer overall survival than those with non-bone metastases, normal hemoglobin levels and normal ferritin levels by the log-rank test, with P values of 0.027, 0.030 and 0.015, respectively. Patients with multiple organ metastases had significantly poorer overall survival than those with single organ metastasis (log-rank P=0.008). In multivariate survival analysis, only whole-body metabolic tumor volume using SUV cut-off value of 2.5 (wMTV) was a significant independent prognostic factor. For wMTV, 870.3 cm was the best cut-off point to discriminate between a good and poor prognosis (log-rank P=0.01).

Conclusions: The systemic F-FDG PET/CT with high sensitivity and specificity has significant advantages in the evaluation of angiosarcoma, particularly in detecting occult metastases. Bone metastases on F-FDG PET/CT, low hemoglobin levels and high ferritin levels were all associated with a poorer prognosis. MTV of the whole body is a significant independent metabolic prognostic factor for overall survival in patients with angiosarcoma.
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http://dx.doi.org/10.21037/qims-21-563DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9014154PMC
May 2022

Role of F-DCFPyL PET/CT in patients with suspected prostate cancer.

Hell J Nucl Med 2022 Jan-Apr;25(1):11-18. Epub 2022 Apr 8.

PET Center, Department of Nuclear Medicine, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China.

Objective: Fluorine-18-2-(3-{1-carboxy-5-[(6-F-flfluoro-pyridine-3-carbonyl)-amino]-pentyl}-ureido)-pentanedioic acid (F-DCFPyL), a novel positron emission tomography/computed tomography (PET/CT) radiotracer that binds to the prostate specific membrane antigen (PSMA), is increasingly used for biochemically recurrent prostate cancer diagnostics. However, the F-DCFPyL characteristics of suspected prostate cancer (SPCa) have been even more rarely described. Herein, in this retrospective study, we describe the clinical impact of F-DCFPyL PET/CT imaging in SPCa.

Subjects And Methods: We retrospectively evaluated the data of 56 SPCa patients who had undergone F-DCFPyL PET/CT studies. These patients were done for primary diagnosis/staging. Positron emission tomography/CT images were analyzed both qualitatively and quantitatively (maximum standardized uptake value (SUVmax) and maximum SUV normalized by lean body mass (SULmax)). Histopathologic diagnosis was taken as reference standard. The optimal cut-off of F-DCFPyL was determined using receiver operating characteristic curve (ROC).

Results: All the patients were confirmed by histopathological examination via prostatectomy or prostate biopsy. Fluorine-18-DCFPyL PET/CT showed higher radiotracer uptake in prostate cancer than that in non-prostate cancer. When SUVmax 5.0 and SULmax 4.0 were cut-off points for determining prostate cancer, the sensitivity of F-DCFPyL was 90%, specificity was 100%, and accuracy was 91.2%. Furthermore, there were highly significant positive correlations between SUVmax, SULmax and serum PSA. On comparison of areas under the curve, no significant difference was seen between SUVmax and SULmax in the sensitivity and specificity of F-DCFPyL PET/CT for PCa identification. However, delayed PET/CT did not improved accuracy in the term of uncertain PCa in the initial standard imaging. As for lymph node staging, the negative predictive value of F-DCFPyL PET/CT was 100%.

Conclusion: Fluorine-18-DCFPyL PET/CT is a promising imaging modality for initial diagnosis and preoperative N staging in SPCa.
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http://dx.doi.org/10.1967/s002449912431DOI Listing
May 2022

Elevated spleen FDG uptake predicts unfavorable outcome in adult idiopathic-inflammatory-myopathy patients: a crisis beyond muscles.

Clin Rheumatol 2022 Jul 19;41(7):2103-2112. Epub 2022 Mar 19.

PET Center, The First Affiliated Hospital, Zhejiang University School of Medicine, #79 Qingchun Road, Hangzhou, Zhejiang Province, People's Republic of China, 310003.

Background: Idiopathic inflammatory myopathy (IIM) is a group of autoimmune diseases that frequently leads to unfavorable outcome. This study aimed at identifying the clinical value of PET/CT scan in predicting the outcome of adult IIM patients.

Methods: Adult IIM patients who were admitted to the four divisions of the First Affiliated Hospital, Zhejiang University School of Medicine (FAHZJU), from January 1, 2017, to December 31, 2020, were retrospectively reviewed. PET/CT scan and other factors of IIM patients who met the inclusion and exclusion criteria were collected and analyzed.

Results: A total of 69 adult IIM patients were finally enrolled into this study. Thirty cases (43.5%) of all the patients enrolled died in follow-up, and the medium follow-up time was 11.90 (4.00, 23.80) months. In particular, 14 patients died within 3 months. The univariate Cox proportional hazards regression analyses revealed pulmonary bacterial infection (P < 0.001), rapidly progressive interstitial lung disease (RP-ILD, P = 0.018), maximum standard uptake value of spleen (spleen SUVmax, P = 0.002), and positivity of anti-MDA5 antibody (P = 0.041) were significantly related to survival in follow-up. The following multivariate Cox proportional hazards regression analysis identified pulmonary bacterial infection (P = 0.003) and spleen SUVmax (P = 0.032) as factors significantly associated with survival of IIM-ILD patients. The subsequent receiver operating characteristic curve analysis showed SUVmax was comparably effective in predicting death within 3 months.

Conclusion: Spleen SUVmax and complication of pulmonary bacterial infection were significantly associated with survival of IIM patients. In addition, elevated spleen SUVmax was efficient in predicting unfavorable outcome of adult IIM patients. Key Points • IIM is a group of autoimmune diseases that frequently leads to unfavorable outcome • Complications of splenic SUVmax and pulmonary bacterial infection were significantly associated with survival in IIM patients.
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http://dx.doi.org/10.1007/s10067-022-06111-4DOI Listing
July 2022

Efficacy and safety of neoadjuvant sintilimab, oxaliplatin and capecitabine in patients with locally advanced, resectable gastric or gastroesophageal junction adenocarcinoma: early results of a phase 2 study.

J Immunother Cancer 2022 03;10(3)

Department of Surgical Oncology, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China

Immune checkpoint inhibitors have greatly improved the prognoses of diverse advanced malignancies, including gastric and gastroesophageal junction (G/GEJ) cancer. However, the role of anti-programmed cell death protein-1 treatment in the neoadjuvant setting remains unclear. This phase 2 study aimed to evaluate sintilimab plus CapeOx as a neoadjuvant regimen in patients with advanced resectable G/GEJ adenocarcinoma. Eligible patients with resectable G/GEJ adenocarcinoma stage cT3-4NanyM0 were enrolled. Patients received neoadjuvant treatment with sintilimab (3 mg/kg for cases <60 kg or 200 mg for those ≥60 kg on day 1) plus CapeOx (oxaliplatin at 130 mg/m on D1 and capecitabine at 1000 mg/m two times per day on D1-D14) every 21 days, for three cycles before surgical resection, followed by adjuvant treatment with three cycles of CapeOx with the same dosages after surgical resection. The primary endpoint was pathological complete response (pCR) rate. Secondary endpoints included objective response rate, tumor regression grade per Becker criteria, survival and safety. As of July 30, 2020, 36 patients were enrolled. Totally 7 (19.4%) patients had GEJ cancer, and 34 (94.4%) patients were clinical stage III cases. A total of 35 (97.2%) patients completed three cycles of neoadjuvant treatment, and 1 patients received two cycles due to adverse events. All patients underwent surgery and the R0 resection rate was 97.2%. In this study, pCR and major pathological response were achieved in 7 (19.4%, 95% CI: 8.8% to 35.7%; 90% CI: 10.7% to 33.1%) and 17 (47.2%, 95% CI: 31.6% to 64.3%) patients, respectively. Thirty-one patients received adjuvant treatment. By December 20, 2021, three patients died after disease relapse, and two patients were alive with relapse. Median disease-free survival (DFS) and overall survival (OS) were not reached. The 1-year DFS and OS rates were 90.3% (95% CI: 80.4% to 100.0%) and 94.1% (95% CI: 86.5% to 100.0%), respectively. The most common (>1 patient) grade 3 treatment-related adverse events during neoadjuvant treatment were anemia and neutropenia (n=5 each, 13.9%). No serious adverse events (AEs) or grade 4-5 AEs were observed. Sintilimab plus oxaliplatin/capecitabine showed promising efficacy with encouraging pCR rate and good safety profile in the neoadjuvant setting. This combination regimen might present a new option for patients with locally advanced, resectable G/GEJ adenocarcinoma. Trial registration; NCT04065282.
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http://dx.doi.org/10.1136/jitc-2021-003635DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8928365PMC
March 2022

The feedback loop of ANKHD1/lncRNA MALAT1/YAP1 strengthens the radioresistance of CRC by activating YAP1/AKT signaling.

Cell Death Dis 2022 02 2;13(2):103. Epub 2022 Feb 2.

Department of General Surgery, Second Affiliated Hospital of Soochow University, Suzhou, 215004, China.

Innate radioresistance substantially limits the effectiveness of radiotherapy for colorectal cancer (CRC); thus, a strategy to enhance the radiosensitivity of CRC is urgently needed. Herein, we reported that ankyrin repeat and KH domain containing 1 (ANKHD1) serves as a key regulator of radioresistance in CRC. ANKHD1 was highly expressed in CRC tissues and was highly correlated with Yes-associated protein 1 (YAP1) in CRC. Our results first revealed that ANKHD1 knockdown could increase the radiosensitivity of CRC by regulating DNA-damage repair, both in vitro and in vivo. Furthermore, the interactive regulation between ANKHD1 or YAP1 and lncRNA MALAT1 was revealed by RIP and RNA pull-down assays. Moreover, our results also demonstrated that MALAT1 silencing can radiosensitize CRC cells to IR through YAP1/AKT axis, similar to ANKHD1 silencing. Taken together, we report a feedback loop of ANKHD1/MALAT1/YAP1 that synergistically promotes the transcriptional coactivation of YAP1 and in turn enhances the radioresistance of CRC by regulating DNA-damage repair, probably via the YAP1/AKT axis. Our results suggested that targeting the YAP1/AKT axis downstream of ANKHD1/MALAT1/YAP1 may enhance the radiosensitivity of CRC.
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http://dx.doi.org/10.1038/s41419-022-04554-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8810793PMC
February 2022

Evidence of Microglial Immune Response Following Coronavirus PHEV Infection of CNS.

Front Immunol 2021 10;12:804625. Epub 2022 Jan 10.

Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China.

Porcine hemagglutinating encephalomyelitis virus (PHEV) is a highly neurotropic coronavirus that invades the host central nervous system (CNS) and causes neurological dysfunction. Microglia are key immune cells in the CNS, however, whether and how they response to PHEV infection remains unclear. Herein, microglial activation and proliferation were detected in the CNS of PHEV-infected mice, as along with the proinflammatory response. Moreover, the production of proinflammatory cytokines induced by moderately activated microglia limited viral replication in the early stage of infection. Microglial depletion assays showed that during late infection, excess activation of microglia aggravated neurological symptoms, BBB destruction, and peripheral monocyte/macrophage infiltration into the CNS. Using an brain slice model, PHEV was identified to specifically and moderately induce microglial activation in the absence of peripheral immune cells infiltration. Consistently, macrophage clearance from circulating blood indicated that peripheral monocytes/macrophages crossing the BBB of mice were responsible for excess activation of microglia and CNS damage in late PHEV infection. Overall, our findings provide evidence supporting a dual role for microglia in the host CNS in response to coronavirus PHEV invasion.
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http://dx.doi.org/10.3389/fimmu.2021.804625DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8784595PMC
February 2022

Trephine-based foraminoplasty in PTED treatment of lumbar lateral recess stenosis.

Adv Clin Exp Med 2022 Apr;31(4):359-367

Department of Orthopedics, The Second Affiliated Hospital of Harbin Medical University, China.

Background: During minimally invasive spine surgery, nerve root decompression is challenging due to the anatomical division and uncertainty in lumbar lateral recess (LLR).

Objectives: To evaluate the outcome and safety of foraminoplasty using percutaneous transforaminal endoscopic decompression (PTED) (performed with an aid of a trephine) in the treatment of lumbar lateral recess stenosis (LLRS).

Material And Methods: All operations were performed under local anesthesia and in prone position. The puncture point was 10-14 cm away from the midline of the spinous process. One hundred eight individuals with LLRS who underwent PTED from September 2016 to December 2020 in our hospital were enrolled in the study. Visual Analog Scale (VAS) and Oswestry Disability Index (ODI) scores were collected preoperatively after 1 day, 7 days, 1 month and at the final follow-up (June 2021). Low back pain and leg pain were measured using VAS score. Functional outcomes were assessed with ODI and modified Macnab criteria.

Results: After the surgery, the VAS score and ODI were statistically significant at all follow-up points compared with the pre-surgery (both p < 0.05). Based on the modified Macnab scores at the final follow-up, the satisfaction rate at postoperative 1 month was 96.3% and the satisfaction rate at postoperative 7 days was 70.38%. A significant difference was observed between the 2 groups (p < 0.05).

Conclusions: Foraminoplasty using PTED performed with a trephine is one of the safe and effective, minimally invasive methods to treat LLRS.
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http://dx.doi.org/10.17219/acem/144638DOI Listing
April 2022

Nano-selenium and Macleaya cordata Extracts Improved Immune Function and Reduced Oxidative Damage of Sows and IUGR Piglets After Heat Stress of Sows in Late Gestation.

Biol Trace Elem Res 2022 Jan 12. Epub 2022 Jan 12.

School of Materials and Architectural Engineering, Guizhou Normal University, Guiyang, 550025, China.

To investigate the effects of nano-selenium (nano-Se) and Macleaya cordata extracts (MCE) on immune function and oxidative damage of sows and intrauterine growth retardation (IUGR) piglets exposed to heat stress (HS) in large-scale farms, a 2 × 2 factorial design was adopted in this test, and the two factors were nano-Se (0, 0.50 mg/kg) and MCE (0, 500 mg/kg). A total of 80 sows ([Landrace × Yorkshire] × Duroc, parity 2) were used in a 25-day trial from day 90 of gestation to delivery with 20 replications per group and 1 sow per replication. The dietary treatments of sows were as follows: (1) CON group, basic diet (0.30 mg/kg added Se, sodium selenite); (2) Nano-Se group, basic diet (0.00 mg/kg added Se) + 0.50 mg/kg added nano-Se; (3) MCE group, basic diet (0.00 mg/kg added Se) + 500 mg/kg added MCE; and (4) Combined group, basic diet (0.00 mg/kg added Se) + 0.50 mg/kg added nano-Se and 500 mg/kg added MCE. The activities of serum SOD, CAT, and GSH-Px of sows and IUGR piglets were significantly increased in MCE group and combined group, and the MDA content was extremely decreased. There were extreme differences in serum IgG level of sows and IUGR piglets, colostrum, and serum IgM level of IUGR piglets in MCE group and combined group compared with CON group. Maternal combined diets increased greatly the levels of serum IL-10 and IFN-γ of sows and IUGR piglets, and decreased extremely the contents of serum IL-1β and TNF-α. MCE alone or combination with nano-Se in sow diets decreased greatly mRNA level of Hsp70 and increased mRNA level of Hsp27 in sows and IUGR piglets. In conclusion, nano-Se and/or MCE can be added to sow diets for the amelioration of HS-induced oxidative damage through improving immune function.
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http://dx.doi.org/10.1007/s12011-022-03103-yDOI Listing
January 2022

Role of immune escape in different digestive tumours.

World J Clin Cases 2021 Dec;9(34):10438-10450

Department of Gastroenterology, The Affiliated Hospital of Zunyi Medical University, Zunyi 563000, Guizhou Province, China.

A counterbalance between immune cells and tumour cells is key to fighting tumours, and immune escape is an important mechanism for the survival of tumour cells in the body. Tumor cells and their cytokines impair the activity of T cells, NK cells, macrophages and other immune cells through various ways, and change the expression of their own surface antigens so as to avoid the clearance of the immune system. Changes in major histocompatibility complex molecules, high expression of programmed death-ligand 1, and the presence of immunosuppressive cells in the tumor microenvironment (TME) are main means by which tumors impair the function of immune cells. During the development of tumours of the digestive system, different mechanisms acting on tumour cells, the TME, and immune cells lead to immune escape and promote tumour progression. In this paper, the mechanisms of immune escape in tumour cells of the digestive system are reviewed to provide a theoretical basis for the immunotherapy of gastrointestinal tumours.
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http://dx.doi.org/10.12998/wjcc.v9.i34.10438DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8686128PMC
December 2021

Locking compression plate fixation of periprosthetic distant humeral fracture after intramedullary nail for humeral shaft fracture: A case report.

Trauma Case Rep 2022 Feb 8;37:100565. Epub 2021 Dec 8.

Guangzhou University of Traditional Chinese Medicine Second Affiliated Hospital, Guangdong Provincial Hospital of Traditional Chinese Medicine, Department of Orthopaedics, Dade Road Number 111, Yuexiu District, Guangzhou City, Guangdong Province 440100, China.

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http://dx.doi.org/10.1016/j.tcr.2021.100565DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8683643PMC
February 2022

Cell-surface glycans act as attachment factors for porcine hemagglutinating encephalomyelitis virus.

Vet Microbiol 2022 Feb 24;265:109315. Epub 2021 Dec 24.

Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China; Key Lab of Animal Production, Product Quality and Security, Ministry of Education, Jilin Agricultural University, Changchun, China. Electronic address:

Porcine hemagglutinating encephalomyelitis virus (PHEV) is a neurotropic coronavirus and highly pathogenic in veterinary clinic. Spike (S) protein of PHEV interplays with host components to cross the plasma membrane of target cells, but characterization of its functional receptors is limited. Here, we discovered that cell-surface glycans, i.e., sialic acid (SA) and heparan sulfate (HS), act as critical interacting factors of PHEV, involving in viral attachment. As shown in glycans depletion assay, removing SA or HS from N2a cells inhibits PHEV infection. Soluble sugar monomers were utilized for competitive binding tests, and we found that both SA and HS could specifically bind to PHEV and affect the viral infectivity. Furthermore, the expression of heparan sulfate proteoglycans (HSPGs), including syndecans and glypicans, and endoglycosidase heparinase which cleaves HS were regulated by PHEV RNA replication. Together, we newly identified specificity recognition of cellular glycans and PHEV during infection, providing novel cellular targets for antiviral therapies and better understanding of pathogenesis.
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http://dx.doi.org/10.1016/j.vetmic.2021.109315DOI Listing
February 2022
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