Publications by authors named "Kui Chen"

203 Publications

Establishment of TUSMi009-A, an induced pluripotent stem cell (iPSC) line from a 24-year-old Chinese Han patient with gliocytoma.

Stem Cell Res 2021 Sep 20;56:102546. Epub 2021 Sep 20.

Department of Neurosurgery, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China. Electronic address:

Glioblastoma is the most common malignant primary brain tumor with poor prognosis and low long-term survival rate. Here we described the production of an iPSC line generated from peripheral blood mononuclear cells (PBMCs) of a 24-year-old female gliocytoma patient. The PBMCs were reprogrammed by the non-integrating Sendai Virus with human OKSM (OCT3/4, SOX2, KLF4, and c-MYC) transcription factors. The generated iPSCs were positive for pluripotent stem cell markers demonstrated by immunocytochemistry. Besides, they displayed a normal karyotype and had the potential to differentiate spontaneously three germ layers in vitro. Our model might be useful in studying the pathogenesis of gliocytoma patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scr.2021.102546DOI Listing
September 2021

Suppression of miR-130a-3p Attenuates Oxygen-Glucose Deprivation/Reoxygenation-Induced Dendritic Spine Loss by Promoting APP.

Front Neurosci 2021 3;15:601850. Epub 2021 Aug 3.

Department of Vascular Surgery, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, Shanghai, China.

Background: Cerebral stroke induces neuronal dysfunction as a consequence of neuronal morphology changes. Emerging evidence suggests that microRNAs (miRNAs) may play an important role in regulating dysfunction in stroke, yet there are still few studies examining the association between whole blood miRNAs and neuronal morphology. The present study aimed to ascertain the potential roles and mechanisms of action of miR-130a-3p in ischemic stroke.

Methods: The miRNA datasets of peripheral serum in the GEO database and the mRNA datasets of the human brain after ischemia were analyzed to identify differentially expressed RNAs, and their functions were verified in cultured neurons . Furthermore, the target gene was validated by dual-luciferase reporter assay, RT-PCR, Western blot, and immunofluorescence experiments. The identified miRNA was further verified by the OGD test to restore neuronal changes after ischemia through APP.

Results: The expression of whole blood miR-130a-3p was found significantly lower in participants with ischemic stroke than in controls by analyzing expression profiling datasets of cerebral ischemia stroke obtained from the Gene Expression Omnibus (GEO) DataSets portal, which was confirmed in the MCAO model in mice. Furthermore, GO analysis showed that miR-130a-3p might directly affect neuronal function. Indeed, we demonstrated that miR-130a-3p played a central role in the inhibition of dendritic morphogenesis and in the growth of dendritic spines . We also confirmed that miR-130a-3p could regulate the expression of by luciferase reporter assay, RT-PCR, Western blot, and immunofluorescence experiments, which were consistent with the bioinformatic analysis. Last but not least, we also demonstrated that reducing miR-130a-3p expression partially rescued neuronal morphological changes after OGD .

Conclusion: miR-130a-3p is a potential biomarker of cerebral stroke, can affect neuronal morphology through , and promote the repair of neurons by promoting expression after cerebral ischemia.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fnins.2021.601850DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8369929PMC
August 2021

Bioinformatics Analysis of Potential Biomarkers and Pathway Identification for Major Depressive Disorder.

Authors:
Dong Qi Kui Chen

Comput Math Methods Med 2021 3;2021:3036741. Epub 2021 Aug 3.

Beijing Friendship Hospital Affiliated to Capital Medical University, China.

Aiming at a more comprehensive understanding of the molecular biomarkers and potential mechanisms of major depressive disorder (MDD), from the Gene Expression Omnibus (GEO) database, we first obtained mRNA expression profiles and identified 585 differentially expressed genes (DEGs) through the R software, including 263 upregulated genes and 322 downregulated genes. Then, through the Kyoto Encyclopedia of Genome and Genome (KEGG) pathway and biological process (BP) analysis, we found that the upregulated and downregulated DEGs were abundant in different pathways, respectively. It was noteworthy that upregulated DEGs were the most significantly enriched in the mTOR signaling pathway. Subsequently, through the protein-protein interaction (PPI) network, we identified seven hub genes, namely, EXOSC2, CAMK2A, PRIM1, SMC4, TYMS, CDK6, and RPA2. Finally, through gene set enrichment analysis (GSEA), we obtained that hypoxia, epithelial-mesenchymal transition, hedgehog signaling, and reactive oxygen species pathway were the enriched pathways for MDD patients. The above data results would provide a new direction for the treatment of MDD patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2021/3036741DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8357473PMC
August 2021

Capicua Regulates Dendritic Morphogenesis Through Ets in Hippocampal Neurons .

Front Neuroanat 2021 27;15:669310. Epub 2021 Jul 27.

Department of Neurosurgery, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China.

Capicua (Cic), a transcriptional repressor frequently mutated in brain cancer oligodendroglioma, is highly expressed in adult neurons. However, its function in the dendritic growth of neurons in the hippocampus remains poorly understood. Here, we confirmed that Cic was expressed in hippocampal neurons during the main period of dendritogenesis, suggesting that Cic has a function in dendrite growth. Loss-of-function and gain-of function assays indicated that Cic plays a central role in the inhibition of dendritic morphogenesis and dendritic spines . Further studies showed that overexpression of Cic reduced the expression of Ets in HT22 cells, while knockdown of Cic in hippocampal neurons significantly elevated the expression of Ets. These results suggest that Cic may negatively control dendrite growth through Ets, which was confirmed by ShRNA knockdown of either Etv4 or Etv5 abolishing the phenotype of Cic knockdown in cultured neurons. Taken together, our results suggest that Cic inhibits dendritic morphogenesis and the growth of dendritic spines through Ets.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fnana.2021.669310DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8353115PMC
July 2021

RECQ1 Promotes Stress Resistance and DNA Replication Progression Through PARP1 Signaling Pathway in Glioblastoma.

Front Cell Dev Biol 2021 26;9:714868. Epub 2021 Jul 26.

Department of Neurosurgery, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai, China.

Glioblastoma (GBM) is the most common aggressive primary malignant brain tumor, and patients with GBM have a median survival of 20 months. Clinical therapy resistance is a challenging barrier to overcome. Tumor genome stability maintenance during DNA replication, especially the ability to respond to replication stress, is highly correlated with drug resistance. Recently, we identified a protective role for RECQ1 under replication stress conditions. RECQ1 acts at replication forks, binds PCNA, inhibits single-strand DNA formation and nascent strand degradation in GBM cells. It is associated with the function of the PARP1 protein, promoting PARP1 recruitment to replication sites. RECQ1 is essential for DNA replication fork protection and tumor cell proliferation under replication stress conditions, and as a target of RECQ1, PARP1 effectively protects and restarts stalled replication forks, providing new insights into genomic stability maintenance and replication stress resistance. These findings indicate that tumor genome stability targeting RECQ1-PARP1 signaling may be a promising therapeutic intervention to overcome therapy resistance in GBM.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fcell.2021.714868DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8350743PMC
July 2021

Bivalirudin versus unfractionated heparin during percutaneous coronary intervention in high-bleeding-risk patients with acute coronary syndrome in contemporary practice.

Biomed Pharmacother 2020 Oct 17;130:110758. Epub 2020 Sep 17.

Department of Cardiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China. Electronic address:

Background: Bivalirudin, as compared with unfractionated heparin (UFH), has been shown to reduce bleeding complications and supply a better safety profile among low/medium-bleeding-risk patients undergoing percutaneous coronary intervention (PCI) for acute coronary syndrome (ACS) in some previous studies. Whether this advantage persists in patients at high risk of bleeding according to contemporary practice characterized by frequent use of radial-artery access and novel P2Y inhibitors, and low use of glycoprotein IIb/IIIa inhibitors (GPIs) is unclear.

Aim Of The Study: This study aimed to assess the efficacy and safety of bivalirudin compared with UFH in high bleeding risk patients with ACS undergoing PCI in current practice.

Materials And Methods: All consecutive high-bleeding-risk patients who underwent PCI for ACS at the First Affiliated Hospital of Zhengzhou University from January to September 2019 were retrospectively analyzed. The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST). Besides, we assessed angina-related health status at 30 days, the length of hospital stay, and hospitalization costs. A logistic regression model was used to adjust for baseline differences. Consistency of the treatment effect of bivalirudin for NACEs and MACEs compared with UFH was evaluated in 15 prespecified subgroups.

Results: From January to September 2019, 823 patients (361 treated with bivalirudin and 462 treated with UFH) were enrolled in the study. GPIs, novel P2Y inhibitors, and radial approach was used in 5.6 %, 66.1 %, and 89.7 % of the patients, respectively. After adjusting for baseline differences, bivalirudin was associated with significant reduction in NACEs, MACEs, major bleeding, and myocardial infarction at 30 days compared with UFH. The individual endpoints of death, stroke, ST and TVR did not differ significantly between the 2 groups after adjusting for covariates. Furthermore, bivalirudin consistently reduced the rates of NACEs and MACEs in the 15 prespecified subgroups compared with UFH. These benefits of bivalirudin can translate into improved angina-related health status, shorter hospital stays, and lower hospitalization costs.

Conclusions: The treatment of bivalirudin showed better efficacy and safety as compared to UFH among patients with ACS undergoing PCI at high risk of bleeding in contemporary practice.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biopha.2020.110758DOI Listing
October 2020

One-year mortality and consequences of COVID-19 in cancer patients: A cohort study.

IUBMB Life 2021 10 29;73(10):1244-1256. Epub 2021 Aug 29.

Department of Emergency Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, 430022, P.R. China.

The 1-year mortality and health consequences of COVID-19 in cancer patients are relatively underexplored. In this multicenter cohort study, 166 COVID-19 patients with cancer were compared with 498 non-cancer COVID-19 patients and 498 non-COVID cancer patients. The 1-year all-cause mortality and hospital mortality rates in Cancer COVID-19 Cohort (30% and 20%) were significantly higher than those in COVID-19 Cohort (9% and 8%, both P < .001) and Cancer Cohort (16% and 2%, both P < 0.001). The 12-month all-cause post-discharge mortality rate in survival discharged Cancer COVID-19 Cohort (8%) was higher than that in COVID-19 Cohort (0.4%, P < .001) but similar to that in Cancer Cohort (15%, P = .084). The incidence of sequelae in Cancer COVID-19 Cohort (23%, 26/114) is similar to that in COVID-19 Cohort (30%, 130/432, P = .13). The 1-year all-cause mortality was high among patients with hematologic malignancies (59%), followed by those who have nasopharyngeal, brain, and skin tumors (45%), digestive system neoplasm (43%), and lung cancers (32%). The rate was moderate among patients with genitourinary (14%), female genital (13%), breast (11%), and thyroid tumors (0). COVID-19 patients with cancer showed a high rate of in-hospital mortality and 1-year all-cause mortality, but the 12-month all-cause post-discharge mortality rate in survival discharged cancer COVID-19 patients was similar to that in Cancer Cohort. Comparing to COVID-19 Cohort, risk stratification showed that hematologic, nasopharyngeal, brain, digestive system, and lung tumors were high risk (44% vs 9%, P < 0.001), while genitourinary, female genital, breast, and thyroid tumors had moderate risk (10% vs 9%, P = .85) in COVID-19 Cancer Cohort. Different tumor subtypes had different effects on COVID-19. But if cancer patients with COVID-19 manage to survive their COVID-19 infections, then long-term mortality appears to be similar to the cancer patients without COVID-19, and their long-term clinical sequelae were similar to the COVID-19 patients without cancer.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/iub.2536DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8426703PMC
October 2021

Dual-Specificity Phosphatase 9 Regulates Cellular Proliferation and Predicts Recurrence After Surgery in Hepatocellular Carcinoma.

Hepatol Commun 2021 Jul 11;5(7):1310-1328. Epub 2021 Mar 11.

Toronto General Hospital Research Institute University Health Network Toronto ON Canada.

Hepatocellular carcinoma (CC) is a common and deadly cancer with complex molecular pathogenesis. Little is known about dual-specificity phosphatases (DUSPs) in HCC. We investigated DUSP9 expression in human HCC, associations between DUSP9 and patient outcomes, and effects of altered DUSP9 expression on HCC biology. We studied public data sets as well as 196 patients at our institution who had HCC resections. Quantitative real-time reverse transcription polymerase chain reaction and western blot demonstrated that expression was increased >10-fold in HCC compared to adjacent liver and healthy controls ( = 0.005). Kaplan-Meier and multivariable regression analyses revealed that higher expression was associated with shorter disease-free survival (high DUSP9, 1.6; 95% confidence interval, 0.9-2.3 vs. low DUSP9, 3.4; 95% confidence interval, 1.8-5.0 years;  = 0.04) and increased risk of recurrence (hazard ratio 1.55; 95% confidence interval, 1.01-2.67;  = 0.05) after resection. complementary DNA (cDNA) was cloned using rapid amplification of cDNA ends, revealing two DUSP9 isoforms in human HCC cells. Studies of transcriptional regulation using promoter-luciferase reporter constructs suggested that DUSP9 transcription is regulated by E26 transformation-specific transcription factors. Proliferation of hepatic cells was enhanced by lentiviral-mediated overexpression of DUSP9. In contrast, DUSP9 knockout HCC cells generated using clustered regularly interspaced short palindromic repeats (CRISPR) demonstrated decreased HCC proliferation and doxorubicin resistance and impaired xenograft growth . RNA sequencing, gene set enrichment, and network/pathway analysis revealed that DUSP9 knockout is associated with activation of protein kinase activity and apoptosis. DUSP9 regulates cell proliferation and predicts recurrence after surgery in HCC. DUSP9 may represent a novel prognostic candidate and therapeutic target. Additional studies are warranted to further explore the role and regulation of DUSP9 in HCC.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/hep4.1701DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8279460PMC
July 2021

The prognostic significance of different proportion of signet-ring cells of colorectal carcinoma.

Bosn J Basic Med Sci 2021 May 29. Epub 2021 May 29.

Department of Colorectal Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China.

While the prognosis of patients with partial SRCC (PSRCC) has been rarely reported, colorectal signet-ring cell carcinoma (SRCC) has been associated with poor prognosis. The aim of this study was to analyze the prognosis of patients with different SRCC composition and establish a prediction model. A total of 91 patients with SRC component were included in the study. These patients were divided into two groups: SRCC group (SRC composition > 50%; n=41) and partial SRCC (PSRCC) group (SRC composition ≤ 50%; n=50). COX regression model was used to identify independent prognostic factors for overall survival (OS). A predictive nomogram was established and compared with the 7th AJCC staging system. After a median follow-up of 16 months, no significant difference in OS was observed in either group. Preoperative carcinoembryonic antigen (CEA) level, pN stage, M stage, preoperative ileus, and adjuvant chemotherapy were independent prognostic risk factors for OS (p<0.05). A nomogram for predicting the overall survival of colorectal SRCC was established with a C-index of 0.800, and it showed better performance than that of the 7th AJCC staging system (p<0.001). In summary, the ratio of SRC component was not an independent prognostic factor of the OS. Those patients with less than 50% of SRC component should be given the same clinical attention. A predictive nomogram for survival based on five independent prognostic factors was developed and showed better performance than the 7th AJCC staging system. This resulted to be helpful for individualized prognosis prediction and risk assessment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.17305/bjbms.2021.5856DOI Listing
May 2021

Source apportionment of PM during different haze episodes by PMF and random forest method based on hourly measured atmospheric pollutant.

Environ Sci Pollut Res Int 2021 Jul 9. Epub 2021 Jul 9.

The State Environmental Protection Key Laboratory of Urban Air Particulate Matter Pollution Prevention and Control, College of Environmental Science and Engineering, Nankai University, Tianjin, 300071, China.

Hourly measured PM-bound species, gases, and meteorological data were analyzed by the PMF receptor model to quantify source contributions, and by the random forest to estimate decisive factors of variations of PM, sulfur oxidation ratio (SOR), and nitrogen oxidation ratio (NOR) during different haze episodes. PM variation was influenced by CO (17%), SO (19%), NH (12%), O (10%), air pressure (P, 9.9%), and temperature (T, 10%) during the whole period. SOR was determined by SO (15%), temperature (T, 9.8%), relative humidity (RHU, 15%), and pondus hydrogenii (pH, 35%), and NOR was influenced by NO (19%), O (14%), NH (13%), and RHU (15%). Three types of pollution episodes were captured. Process I was characterized by high CO (contributing 40% of PM concentration variation estimated by the random forest) due to coal combustion for heating during winter in northern China. According to the PMF, coal combustion (32%) and secondary sources (38%) were both the most important contributors in the first stage, and then, when the RHU increased to above 80%, the highest contribution was from secondary sources (40%). Process II was during the Spring Festival and was characterized by 8.8 μg m firework contribution. High SO during this process, especially on the CNY's Eve, was observed due to the firework displays, and SO gave a high contribution (24%) to PM variation. Process III showed high ions and high RHU in summer with sulfate and nitrate contributing 44% and 22%, respectively. Furthermore, meteorological parameters and NH play a key role on SOR and NOR.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11356-021-14487-0DOI Listing
July 2021

The Aggregation Induced Emission Probe of Detecting Enhanced Permeation and Retention Effects is Structured for Evaluating the Applicability of Nanotherapy to Different Tumor Individuals.

J Nanosci Nanotechnol 2021 12;21(12):6054-6059

CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China.

Enhanced permeation and retention (EPR) effect, the mechanism by which nanodrugs accumulate in tumors and acquire superior curative effect. The questions of these mechanisms occur because of limited clinical transformation of engineered nanomaterials after 30 years. The difference of EPR limits the therapeutic effect of nanodrugs in the individual patient. Evaluation of the EPR effect in the individual patient will aid in selecting patients who will accumulate higher amounts of nanotherapeutics and show better therapeutic efficacy. Based on varied TIMP1/MMP-9 in serum, an aggregation-induced emission luminogen probe was designed and constructed to detect and evaluate the EPR effect in model mouse. The result showed that the ratio of TIMP1/MMP-9 (in the range 0.2-1.2) and fluorescence intensity of the probe were negative linear correlation and the effects of BSA-rhodamine accumulation in tumor were individualized differences as well as correlated with the relative ratio of TIMP-1/MMP-9 in serum. Our data support the development of these biomarkers probes based on the personalized nanotherapy of tumor.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1166/jnn.2021.19524DOI Listing
December 2021

Evaluation of Nano-Particulate-Matter-Induced Lung Injury in Mice Using Quantitative Micro-Computed Tomography.

J Nanosci Nanotechnol 2021 12;21(12):6041-6047

College of Food Science, Shihezi University, Shihezi, 832000, China.

Nano-particulate matters (NPM) induced the lung injury in mice were evaluated using quantitative micro-computed tomography in the present article. It is an important negative effect of health problems that NPM exposure provokes changes in the lung injury. The micro-computed tomography (CT) to assess lung injury in mouse models has been investigated. The dynamic structural changes in a NPM-induced lung injury mouse mode were monitored. Adults female BALB/C mice were repeatedly exposed to NPM, and micro-CT scans were performed at day 0, 3, 5 and 9. Lung samples were also collected for histological analysis at each time point. The total lung volume, the injured lung volume, and the normal lung volume were defined and calculated volume during the phase of NPM-exposure on the mice. The total and injured lung volumes of NPM-exposed mice were significantly larger than those of the mice at day 5 and 9. The data from micro-CT was consistent with alveolar enlargement and destruction by histological quantification from pathological section. The study for NPM-induced lung injury model by micro-CT may extend our understanding of the distinct pathophysiology of NPM induced lung injury in mice.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1166/jnn.2021.19522DOI Listing
December 2021

Viral mimetic poly(I:C) induces neutrophil extracellular traps via PAD4 to promote inflammation and thrombosis.

Biochem Biophys Res Commun 2021 Aug 4;565:64-71. Epub 2021 Jun 4.

Department of Cardiology, The 2nd Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China. Electronic address:

Neutrophil extracellular traps (NETs) are extracellular webs of DNA, histones and granular contents that are released by neutrophils to control infections. However, NETs that is not properly regulated can propagate inflammation and thrombosis. It was recognized that viruses can induce NETs. As a synthetic analog of viral double-stranded (ds) RNA, polyinosinic-polycytidylic acid [poly(I:C)] is known to induce inflammation and thrombosis. However, whether and how poly(I:C) modulates NETs remains unclear. Here, we have demonstrated that poly(I:C) induced extracellular DNA traps in human neutrophils in a dose-dependent manner. Further, poly(I:C) or dsRNA virus elevated the levels of myeloperoxidase-DNA complexes and citrullinated histone H3, which are specific markers of NETs, in both neutrophil supernatants and mouse plasma. Interestingly, a potent peptidylarginine deiminase 4 (PAD4) inhibitor, BB-CL-Amidine (BB-CLA) or PAD4 knockdown effectively prevented poly(I:C)-induced NETs formation and release. In addition, BB-CLA abrogated poly(I:C)-triggered neutrophil activation and infiltration, and vascular permeability in lungs. BB-CLA also attenuated poly(I:C)-induced thrombocytopenia in circulation, fibrin deposition and thrombus formation in tissues. Taken together, these results suggest that viral mimetic poly(I:C) may induce NETs-dependent inflammation and thrombosis through PAD4, and that inhibiting PAD4 may become a good strategy to protect against viral infection-caused inflammation/thrombosis-related pathological conditions of diseases.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbrc.2021.05.091DOI Listing
August 2021

[Formation and Prevention of Secondary Nitrate in PM in Tianjin].

Huan Jing Ke Xue 2021 Jun;42(6):2616-2625

Tianjin Eco-Environmental Monitoring Center, Tianjin 300191, China.

To study the formation and approaches to controlling secondary nitrate in PM, the ionic compositions of PM, pH of aerosols, variations in NH-NH and HNO-NO concentrations, and the joint NH/HNO sensitivity regime map of ammonium nitrate were investigated based on high-resolution online monitoring data for an urban site in central Tianjin from 2018 to 2019. The results showed that the average concentration of PM was 58μg·m, and the main ionic compositions of PM were nitrate (NO), ammonium (NH), sulfate (SO), Cl, and K with corresponding mass percentages of 18.4%, 11.6%, 10.3%, 3.3%, and 2.6%, respectively. Concentrations of PM and the main components were relatively high during the heating season and relatively low during the non-heating season. The aerosols were weakly acidity with an average pH of 5.21; pH was higher in spring and winter and lower in summer and autumn, and diurnal variations pH were lower in the morning (00:00-08:00) and slightly higher at other times. The concentrations of NH(g) (gas NH) and HNO(g) (gas HNO) were 16.7μg·mand 1.2μg·m, respectively. The concentrations of NH(g) were relatively higher from April to September and lower from October to February of the following year. HNO(g) concentrations did not show any clear monthly pattern. Except during the summer, NH(g) concentrations were higher in the morning and evening, and HNO(g) concentrations were higher during the day. No clear linear relationships were observed between the concentrations of NH(g) and NH nor the concentrations of HNO(g) and NO at different pH levels. Higher concentrations of NO and NH were observed in the morning and evening, while no linear relationships were observed between the pH and concentrations of NH(g)-NH and HNO(g)-NO. The joint NH/HNO sensitivity regime map showed that most of the points were located in the HNO sensitive region with some in the NH & HNO sensitive region. In spring, autumn, and winter, most of the points were located in the HNO sensitive region while in summer, a significant quantity of the points were located in the NH & HNO sensitive region. Therefore, the precursors of HNO (such as NO) should be controlled in the spring, autumn, and winter, and attention should be given to the control of the precursors of HNO (NO) and NH in the summer to effectively control nitrate and ammonium aerosols in PM in Tianjin.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.13227/j.hjkx.202010119DOI Listing
June 2021

Concentration and atmospheric transport of PM-bound polycyclic aromatic hydrocarbons at Mount Tai, China.

Sci Total Environ 2021 Sep 5;786:147513. Epub 2021 May 5.

Collaborative Innovation Center on Forecast and Evaluation of Meteorological Disasters, Key Laboratory for Aerosol-Cloud-Precipitation of China Meteorological Administration, Nanjing University of Information Science & Technology, Nanjing 210044, China.

Atmospheric PM-bound polycyclic aromatic hydrocarbons (PAHs) pose a major threat to human health. At present, studies on PAHs in the atmosphere have mostly focused on their concentration levels and source apportionment, whereas studies on the vertical transport of PAHs in the atmosphere are limited. However, the vertical transport of PAHs is important for their diffusion near the ground and their long-range transport at higher altitude. In this study, PM samples were collected simultaneously at the summit and foot of Mount Tai (MT and MT, respectively) from May to June 2017, and the concentrations of 18 PAHs in the samples were determined. The total concentration of PAHs at MT was 2.406 ng m, which was well below the pollution levels of domestic cities, whereas that at MT was as high as 9.068 ng m, which was within the range of pollution levels in domestic cities. The total carcinogenic risk for both MT and MT was within the potential risk range. Given the source of PAHs and the diurnal variation of the planetary boundary layer, the PAHs showed opposite diurnal trends at MT and MT. Vertical transport was an important source of daytime PAHs at MT, and the vertical transport efficiency of PAHs decreased with an increasing ring number; this may be due to the combined effects of gas-particle partitioning and chemical reactions. Furthermore, PAHs originating in the surrounding high-emission provinces can affect the Mount Tai area via atmospheric trans-regional transport, and the BaP/BeP ratio is a useful indicator of the transport distance of PAHs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scitotenv.2021.147513DOI Listing
September 2021

Increased Production of Short-Chain Fatty Acids in Microbacteria Fermentation Treated by Fullerenols.

J Nanosci Nanotechnol 2021 10;21(10):5352-5362

CAS Key Laboratory for Biomedical Effects of Nanomaterial and Nanosafety, Institute of High Energy Physics, Chinese Academy of Science (CAS), Beijing 100049, China.

Fullerenol nanoparticles were found to significantly modulate the gut microbiota and selectively enrich the short-chain fatty acids (SCFAs) production by adjusting the gut microbacteria in mice models. In this research, we screened the from seven strains and investigated the interactions and mechanism between the and fullerenol NPs fermentation. The results shows that fullerenol NPs increased the amounts of acetate and butyrate of without significant bacteria growth in the complete medium. The activities of the butyryl-CoA: acetate CoA transferase (BUT), which are the main pathway to produce butyrate, were reduced while the activities of the butyrate kinase (BUK) were enhanced simultaneously. Surprisingly, fullerenol NPs promoted the growth of and in low glucose medium, but they could not be direct carbon source in the culture. Moreover, when cocultured with and the bifidobacterial strains in fullerenols, the biomass and acetate production of were markedly increased while butyrate was decreased significantly.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1166/jnn.2021.19341DOI Listing
October 2021

Efficacy and Safety of Bivalirudin During Percutaneous Coronary Intervention in Chronic Total Occlusion: A Retrospective Study.

Clin Ther 2021 05 31;43(5):844-851. Epub 2021 Mar 31.

Department of Cardiology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China. Electronic address:

Purpose: Bivalirudin as a thrombin inhibitor is proven to have a low risk of bleeding during percutaneous coronary intervention (PCI). Some evidence indicates comparable effectiveness and safety between bivalirudin and unfractionated heparin (UFH). Although bivalirudin during PCI offers more clinical and safety benefits to patients with chronic total occlusion (CTO), mostly via radial access, this has not been confirmed. The objective of this study was to examine the efficacy and safety of bivalirudin during percutaneous coronary intervention (PCI) in patients with CTO.

Methods: This trial used a retrospective cohort study design. Medical information from 736 patients with CTO who underwent PCI with bivalirudin or UFH at the First Affiliated Hospital of Zhengzhou University from July 2019 to September 2020 was extracted and analyzed. The primary end point was the 30-day incidence of net adverse clinical events (NACEs), and the secondary end point was the major adverse cardiovascular events (MACEs), which were related to safety and efficacy, respectively. Other end points incorporated each component of the primary outcome, target vessel revascularization, and stent thrombosis. Clinical and procedural characteristics at baseline were adjusted by using a logistic regression model.

Findings: Overall, 71.5% of patients with CTO used the radial approach. Both groups exhibited nonsignificant differences in the majority of baseline characteristics. The bivalirudin group was associated with a significant reduction in NACEs (12.9% vs 21.5%; P = 0.002) and major bleeding (2.5% vs 8.0%; P = 0.001) versus the UFH group at the end of the 30-day follow-up. The incidence of MACEs, myocardial infarction, death, stroke, stent thrombosis, and target vessel revascularization did not differ significantly between the 2 groups. Moreover, the bivalirudin group also reported a lower incidence of NACEs in the prespecified subgroups.

Implications: Bivalirudin exhibited comparative efficacy but superior safety compared with UFH among patients with CTO undergoing PCI. Chinese Clinical Trial Registry: ChiCTR2000034771.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.clinthera.2021.03.004DOI Listing
May 2021

[Distribution Characteristics and Source Analysis of Water-soluble Ions in Particulate Matter Under Different Weather Processes in Nanjing].

Huan Jing Ke Xue 2021 Feb;42(2):564-573

Key Laboratory for Aerosol-Cloud-Precipitation of China Meteorological Administration, Collaborative Innovation Center on Forecast and Evaluation of Meteorological Disasters, Nanjing University of Information Science & Technology, Nanjing 210044, China.

From November 16 to 28 2018, water-soluble ions in particulate matter and some trace gases in Nanjing City were observed using the online gas composition and aerosol monitoring system MARGA ADI 2080. Combined with meteorological elements and sounding data, the distribution characteristics and day-night differences of pollutants and water-soluble ions during haze, fog, clear, and precipitation processes were analyzed. The results show that the average concentration of PM varied from 26.9μg·m (precipitation) to 96.4μg·m (haze) while total water-soluble ions varied between 23.7μg·m (precipitation) and 89.7μg·m (haze). The ranked order of ion concentrations was NO > NH > SO > Cl > K > Ca > Na > Mg during haze and fog events, and NO > SO > NH > Cl > Ca > K > Na > Mg during clear weather and precipitation period. The diurnal distributions of water-soluble ions were quite different under the four conditions, although SO, NO, and NH(SNA) were ranked haze > fog > clear > precipitation for both day and night periods. According to the PMF source analysis, secondary sources were the main factors affecting haze; secondary sources, sea salt, and combustion sources were the main pollution sources to foggy conditions; and the removal effect of precipitation on coal-fired sources and secondary sources was more notable than during clear conditions.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.13227/j.hjkx.202005317DOI Listing
February 2021

Dexmedetomidine alleviates myocardial ischemia/reperfusion-induced injury and Ca overload via the microRNA-346-3p/CaMKIId axis.

Int J Cardiol 2021 09 14;338:185-195. Epub 2021 Mar 14.

Department of Cardiology, The First Affiliated Hospital of Zhengzhou University, No. 1, Jianshe Road, Erqi District, Zhengzhou 450000, Henan, China.

Myocardial ischemia/reperfusion (MI/R) may impair cardiac functions. Dexmedetomidine (DEX) is protective in various clinical cases. Therefore, this study investigated the role and mechanism of DEX in MI/R. The myocardial infarct size, apoptosis, and levels of myocardial enzymes, SOD, ROS, Ca, and inflammatory factors in DEX-treated MI/R rats were measured. Differentially expressed microRNAs (miRs) in DEX-treated MI/R rats were detected. miR-346-3p was intervened to assess the effects of DEX on MI/R rats. The targeted binding relationship between miR-346-3p and CaMKIId was predicted and verified. DEX effect on hypoxia/reoxygenation (H/R)-induced cell model was evaluated. The role of CaMKIId in DEX protection was assessed after CaMKIId overexpression in H/R cells. NF-κB pathway and NLRP3 inflammasome-related protein levels were detected. DEX alleviated the myocardial injury and Ca overload in MI/R rats, as evidenced by reduced infarct size, apoptosis and levels of myocardial enzymes, ROS, Ca, and inflammatory factors. DEX promoted miR-346-3p expression in MI/R rats, and miR-346-3p knockdown reversed DEX protection on MI/R rats. miR-346-3p targeted CaMKIId. DEX improved H/R-induced cell injury and Ca overload and inhibited NF-κB/NLRP3 inflammasome-related protein levels, which were all reversed by CaMKIId overexpression. DEX alleviated injury and Ca overload in MI/R via regulating the miR-346-3p/CaMKIId axis and inhibiting the NF-κB/NLRP3 inflammasome pathway.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ijcard.2021.03.016DOI Listing
September 2021

Study on Dissolution Thermodynamics and Cooling Crystallization of Rifamycin S.

ACS Omega 2021 Feb 25;6(5):3752-3762. Epub 2021 Jan 25.

School of Chemical Engineering, East China University of Science and Technology, Shanghai 200237, China.

The solubility data of rifamycin S were measured in isopropanol, butyl acetate, and their mixed solvents across the temperature range of 283.15-323.15 K by the gravimetric method. The results demonstrate that the solubility of rifamycin S increases with the increasing temperature in the two pure solvents, and in the mixed solvents, it increases first and then decreases with increasing butyl acetate content. The experimental data of rifamycin S in the mixed solvents were better correlated using the modified Apelblat equation and ideal model equation. Furthermore, the relevant thermodynamic parameters of the dissolution process were determined based on the van't Hoff equation. The obtained dissolution enthalpy and Gibbs free energy are positive in all cases, which indicate that the dissolving process of rifamycin S is endothermic and nonspontaneous. The supersolubility data of rifamycin S were measured by the laser and thermal analytic method. The results demonstrate that the width of the metastable zone of rifamycin S becomes larger with decreasing cooling rate and increasing butyl acetate content. Furthermore, the crystallization process of rifamycin S was optimized on the basis of thermodynamic research. The results showed that when : was 0.04, the cooling rate was 0.1 K/min, the stirring rate was 150 rpm, the final crystallization temperature was 283.15 K, and the aging time was 8 h, the purity of rifamycin S crystals could reach 98.5%, and the crystalline yield was 89.6%. After crystallization optimization, the size of rifamycin S crystals increased, and the dissolution in water was improved.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsomega.0c05337DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7876849PMC
February 2021

Ultrathin two-dimensional Fe-doped cobaltous oxide as a piezoelectric enhancement mechanism in quartz crystal tuning fork (QCTF) photodetectors.

Opt Lett 2021 Feb;46(3):496-499

An innovative ultrathin two-dimensional (2D) Fe-doped cobaltous oxide (Fe-CoO) coated quartz crystal tuning fork (QCTF) was introduced for the purpose of developing a low-cost photoelectric detector with a simple configuration. The enhancement mechanism of the piezoelectric signal in the ultrathin 2D Fe-CoO-coated QCTF detector is assumed to be the synergetic photocarrier transfer and photothermal effect of ultrathin 2D Fe-CoO. The ultrathin 2D nanosheet structure of Fe-CoO with a large specific surface area can efficiently absorb and convert light into heat in the QCTF, and the photocarrier transfer from the Fe-CoO nanosheet to the electrode of the QCTF contributes to the enhancement in electricity given the shortened diffusion distance of carriers to the surfaces of the 2D nanosheet. Finite element modeling was adopted to simulate the thermoelastic expansion and mechanical resonance of the QCTF with 2D Fe-CoO coating to support experimental results and analyses. Moreover, the effects of 2D Fe-CoO on the performance of QCTF-based photoelectric detectors were investigated. This Letter demonstrates that ultrathin 2D materials have great potential in applications such as costly and tiny QCTF detectors, light sensing, biomedical imaging, and spectroscopy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1364/OL.406103DOI Listing
February 2021

In reply to the letter to the editor regarding "TGM6 variants in Parkinson's disease: clinical findings and functional evidence".

J Integr Neurosci 2020 12;19(4):739-740

Department of Neurology, Shanghai Tenth People's Hospital, Tongji University, School of Medicine, 301 Middle Yanchang Road, Shanghai, 200072, P. R. China.

No abstract present.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.31083/j.jin.2020.04.283DOI Listing
December 2020

[Characterization and Source Apportionment of Atmospheric VOCs in Tianjin in 2019].

Huan Jing Ke Xue 2021 Jan;42(1):55-64

Tianjin Eco-Environmental Monitoring Center, Tianjin 300191, China.

The characterization and source apportionment of atmospheric volatile organic compounds (VOCs) in Tianjin in 2019 were investigated based on high-resolution online monitoring data observed at an urban site in Tianjin. The results showed that the average annual concentration of VOCs was 48.9 μg·m, and seasonal concentrations followed with winter (66.9 μg·m) > autumn (47.9 μg·m) > summer (42.0 μg·m) > spring (34.6 μg·m). The chemical compositions of the VOCs were alkanes, aromatics, alkenes, and alkynes, which accounted for 65.0%, 17.4%, 14.6%, and 3.0% of the VOCs concentrations on average, respectively. The proportion of alkanes, aromatics, and alkynes was the highest in autumn, summer, and winter, respectively, while a higher alkenes proportion was observed in summer and winter. The ozone formation potential contribution of alkanes, alkenes, aromatics, and alkynes in spring and summer was 16.9%, 48.6%, 33.5%, and 1.0%, respectively, and the species with higher contributions were ethene, propylene, ,-xylene, 1,2,3-trimethylbenzene, toluene, isoprene, trans-2-butene, cis-2-pentene, -xylene, and -ethyltoluene. During autumn and winter, the aromatics contributed as much as 91.5% to the secondary organic aerosol (SOA) formation potential, and -xylene, toluene, ,-xylene, ethylbenzene, -ethyltoluene, and benzene were the main contributing species. Positive matrix factorization was applied to estimate VOCs source contributions, and automobile exhaust, liquefied petroleum gas/natural gas (LPG/NG) and gasoline evaporation, solvent usage, petrochemical industrial emissions, combustion, and natural sources were identified as major sources of VOCs in spring and summer, accounting for 29.2%, 19.9%, 16.4%, 10.3%, 7.3%, and 6.6%, respectively. While in autumn and winter, the contributions of LPG/NG and gasoline evaporation, automobile exhaust, combustion, solvent usage, and petrochemical industrial emissions were 32.4%, 21.9%, 18.5%, 13.3%, and 8.4%, respectively. Compared to the source contributions in spring and summer, a significant increase was observed for LPG/NG and combustion emission of 62.8% and 153.4%, respectively, and other sources decreased by 18.4%-25.0% in autumn and winter. Source composition spectrums showed that the petrochemical industry and solvent usage were the main emission sources of alkenes and aromatics in spring and summer, and combustion and solvent usage were the main emission sources of aromatics in autumn and winter. Thus, focus should be played on the petrochemical industry and solvent usage in spring and summer and on combustion and solvent usage in autumn and winter to further prevent and control ozone and SOA in Tianjin.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.13227/j.hjkx.202006257DOI Listing
January 2021

Development of a High-Throughput Affinity Mass Spectrometry (AMS) Platform Using Laser Diode Thermal Desorption Ionization Coupled to Mass Spectrometry (LDTD-MS).

SLAS Discov 2021 Feb 17;26(2):230-241. Epub 2020 Dec 17.

Discovery Technologies, Thousand Oaks, CA, USA.

Affinity selection mass spectrometry (MS) or, simply, affinity mass spectrometry (AMS) is a label-free technology that has been used to identify high-affinity ligands of target proteins of interest by screening against small-molecule compound libraries and identifying molecules that are enriched in the presence of the target protein. We have previously applied Agilent Technology's (Santa Clara, CA) RapidFire solid-phase extraction (SPE)-based high-throughput MS technology to screen small-molecule libraries using AMS. However, SPE-based technologies rely on fluidics for desalting and separation prior to mass analysis with attendant high solvent consumption, relatively high sample volume requirements, risk of sample carryover, and frequent maintenance. To address these challenges, we have established an AMS platform using a laser diode thermal desorption-atmospheric pressure chemical ionization (LDTD-APCI) ionization source (Phytronix, Quebec, Canada) coupled with a SCIEX 5600+ TripleTOF MS (Framingham, MA). We also validated a data-independent acquisition (DIA) Sequential Window Acquisition of All Theoretical Mass Spectra (SWATH-MS) method for the robust detection and analysis of small-molecule affinity hits. An informatics platform developed in-house has resulted in a streamlined data analysis workflow for high-throughput AMS screening campaigns and reduced data processing time without compromising data quality. Finally, 68,000 compounds were screened in a single plate and affinity selected hits were confirmed in an orthogonal enzyme activity assay.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1177/2472555220979596DOI Listing
February 2021

Enhancer of zeste homolog 2 participates in the process of atherosclerosis by modulating microRNA-139-5p methylation and signal transducer and activator of transcription 1 expression.

IUBMB Life 2021 01 17;73(1):238-251. Epub 2020 Dec 17.

Department of Cardiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Atherosclerosis (AS) is the main cause of coronary heart disease, in which enhancer of zeste homolog 2 (EZH2) has been implied to participate in this process. Thus, this work proposed to explore the effect of EZH2 on AS from microRNA-139-5p (miR-139-5p)/signal transducer and activator of transcription 1 (STAT1) axis. EZH2, miR-139-5p, and STAT1 expression in arterial tissues of AS patients were detected. Human arterial smooth muscle cells (HASMCs) induced with oxidized low-density lipoprotein (ox-LDL) and the mice fed with high fat diet were treated with silenced EZH2 or upregulated miR-139-5p to explore their roles in proliferation and apoptosis of HASMCs, together with inflammation response and oxidative stress of mice. Chromatin immunoprecipitation experiment was applied to verify the regulatory effect of EZH2 on miR-139-5p through methylation of H3K27me3. The targeting relationship between miR-139-5p and STAT1 was verified by online website and luciferase activity assay. Reduced miR-139-5p and overexpressed EHZ2 and STAT1 were found in AS. Silenced EZH2 or elevated miR-139-5p decreased the production of cholesterol and inhibited inflammation reaction in serum of mice with AS. Silenced EZH2 or elevated miR-139-5p facilitated proliferation and restrained apoptosis of ox-LDL-treated HASMCs, and restrained oxidative stress and cell apoptosis in arterial tissues of AS mice. EZH2 regulated miR-139-5p through H3K27me3, and miR-139-5p targeted STAT1. miR-139-5p silencing antagonized the effects of EZH2 down-regulation on AS. This study manifests that down-regulated EZH2 or elevated miR-139-5p inhibits ox-LDL-induced HASMCs apoptosis, plaque formation, and inflammatory response in AS mice, which may be related to down-regulated STAT1.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/iub.2423DOI Listing
January 2021

Highly Branched Gradient Glycopolymer: Enzyme-Assisted Synthesis and Enhanced Bacteria-Binding Ability.

Biomacromolecules 2020 12 1;21(12):5233-5240. Epub 2020 Dec 1.

Center for Soft Condensed Matter Physics and Interdisciplinary Research, School of Physical Science and Technology, Soochow University, Suzhou 215006, P. R. China.

A one-pot strategy was applied to synchronize enzymatic monomer transformation with reversible addition fragmentation chain transfer (RAFT) polymerization for the synthesis of glycopolymers with highly branched gradient architectures. Also, the linear analogues, block glycopolymers, and gradient glycopolymers were also synthesized for comparison. The binding ability of glycopolymers toward bacteria was then studied by optical density (OD) test, confocal laser scanning microscopy (CLSM), and quartz crystal microbalance with dissipation (QCM-D). The results show that the highly branched gradient glycopolymers have the most remarkable bacteria-binding ability compared with the two linear analogues, gradient glycopolymers, and block glycopolymers. The highly branched glycopolymers were further used as inhibitors in the anti-infection test, demonstrating a significant inhibitory effect on preventing bacteria from infecting the cells.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.biomac.0c01311DOI Listing
December 2020

Improved mechanical, water vapor barrier and UV-shielding properties of cellulose acetate films with flower-like metal-organic framework nanoparticles.

Int J Biol Macromol 2021 Jan 27;167:1-9. Epub 2020 Nov 27.

Guangxi Key Laboratory of Electrochemical and Magneto-chemical Functional Materials, College of Chemistry and Bioengineering, Guilin University of Technology, Guilin 541004, China. Electronic address:

Flower-like metal-organic frameworks (Cu-MOF) nanoparticles are successfully synthesized and incorporated into cellulose acetate (CA) matrix to prepare CA-based functional nanocomposite films via a simple solution-casting method. The effect of the incorporation of flower-like Cu-MOF on the morphological, mechanical, thermal, surface wettability, water vapor barrier, cytotoxicity, photostability and UV-shielding properties of CA films is fully investigated. Results reveal that the flower-like Cu-MOF has good compatibility with CA, providing uniform and compact nanocomposite films. The as-prepared nanocomposite films show improved mechanical properties, surface hydrophobicity, water vapor barrier ability compared to neat CA film, and exhibit super UV-shielding capability through the entire UV regions meanwhile retaining a high visible transparency. Moreover, the high transparency and UV-shielding ability of the nanocomposite films can be still maintained even after continuous UV-light (365 nm) irradiation for 12 h. In addition, MTT cytotoxicity assays towards normal human liver cells (HL-7702) reveal high cell viability (over 80%) and good biocompatibility for the CA/Cu-MOF nanocomposite films. These results indicate that the CA/Cu-MOF nanocomposite films with obviously improved physical and functional performances hold significant potential for transparent packaging and UV-protection applications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ijbiomac.2020.11.164DOI Listing
January 2021

Association between MMP16 rs60298754 and clinical phenotypes of Parkinson's disease in southern Chinese.

Neurol Sci 2021 Aug 25;42(8):3211-3215. Epub 2020 Nov 25.

Department of Neurology, Shanghai East Hospital, Tongji University School of Medicine, 1800 Yuntai Road, Shanghai, 200123, China.

Study Objectives: The aim was to investigate the association between MMP16 rs60298754 and symptoms of Parkinson's disease (PD) in southern Chinese.

Methods: Seven hundred forty-five PD patients were recruited in this study. All patients were evaluated by Brief Pain Inventory (BPI), Hamilton anxiety rating scale and Hamilton depression rating scale, 39-item Parkinson's disease Questionnaire (PDQ-39), and MDS-Unified PD Rating Scale (MDS-UPDRS). Symptoms were also recorded.

Results: The difference of BPI and Parkinson's disease sleep scale (PDSS) between two groups was showed (BPI: MMP16 wildtypes: 14.73 ± 14.45; MMP16 carriers: 10.95 ± 10.67, p 0.002; PDSS: MMP16 wildtypes: 117.80 ± 21.45; MMP16 carriers: 108.40 ± 23.95, p < 0.001). The association of apathy, nocturia, and sensitive to light were found (apathy: p 0.001, OR: 0.49, 0.32-0.76; nocturia: p < 0.001, OR: 3.57, 1.90-7.26; sensitive to light: p < 0.001, OR: 3.99, 2.01-7.74).

Conclusions: MMP16 rs60298754 was associated with the presence of apathy, pain, nocturia, and sensitive to light.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10072-020-04894-5DOI Listing
August 2021

The BRD4 inhibitor JQ1 protects against chronic obstructive pulmonary disease in mice by suppressing NF-κB activation.

Histol Histopathol 2021 Jan 20;36(1):101-112. Epub 2020 Nov 20.

Department of Emergency Medicine, Affiliated Dongfeng Hospital, Hubei University of Medicine, Shiyan, Hubei, China.

Objective: To examine the effect of the BRD4 inhibitor JQ1 on mice with chronic obstructive pulmonary disease (COPD) via NF-κB.

Methods: COPD models constructed by exposure to cigarette smoke and intratracheal instillation of lipopolysaccharides (LPS) in mice were treated with JQ1 (15, 25 or 50 mg/kg). HE staining was performed to observe histopathological changes in the lung tissues. Enzyme-linked immunosorbent assays (ELISAs) were used to measure the levels of IL-10, IFN-γ, IL-17, IL-1β, IL-6, TNF-α, MMP-2, MMP-9, MDA, SOD, T-AOC and HO-1, and gelatin zymography assays were used to examine MMP-2 and MMP-9 activity. A TransAMTM NF-κB p65 detection kit was used to test NF-κB p65/DNA binding activity. Western blotting was conducted to analyze NF-κB p65 in the nucleus and its acetylation.

Results: JQ1 dose-dependently improved the histopathological changes in the lung tissues and decreased the mean linear intercept (MLI), destructive index and inflammatory score of the mice with COPD. The mice with COPD showed increased levels of MMP-2, MMP-9, IFN-γ, IL-17, IL-1β, IL-6 and TNF-α with decreased IL-10 level; these changes were reversed by JQ1 in a dose-dependent manner. In addition, JQ1 reduced the MDA level and increased the SOD, HO-1 and T-AOC levels in mice with COPD, with suppression of NF-κB p65 expression in the nucleus, NF-κB/p65 (Lys310) acetylation and NF-κB p65/DNA binding activity in the lung tissues.

Conclusion: The BRD4 inhibitor JQ1 can downregulate MMP-2 and MMP-9 expression, reduce inflammatory responses, and alleviate oxidative stress in mice with COPD, and this mechanism might be related to the inhibition of NF-κB.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.14670/HH-18-283DOI Listing
January 2021

Two machine learning methods identify a metastasis-related prognostic model that predicts overall survival in medulloblastoma patients.

Aging (Albany NY) 2020 11 5;12(21):21481-21503. Epub 2020 Nov 5.

Department of Neurosurgery, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, P.R. China.

Approximately 30% of medulloblastoma (MB) patients exhibit metastasis at initial diagnosis, which often leads to a poor prognosis. Here, by using univariate Cox regression analysis, two machine learning methods (Lasso-penalized Cox regression and random survival forest-variable hunting (RSF-VH)), and multivariate Cox regression analysis, we established two metastasis-related prognostic models, including the 47-mRNA-based model based on the Lasso method and the 21-mRNA-based model based on the RSF-VH method. In terms of the results of the receiver operating characteristic (ROC) curve analyses, we selected the 47-mRNA metastasis-associated model with the higher area under the curve (AUC). The 47-mRNA-based prognostic model could classify MB patients into two subgroups with different prognoses. The ROC analyses also suggested that the 47-mRNA metastasis-associated model may have a better predictive ability than MB subgroup. Multivariable Cox regression analysis demonstrated that the 47-mRNA-based model was independent of other clinical characteristics. In addition, a nomogram comprising the 47-mRNA-based model was built. The results of ROC analyses suggested that the nomogram had good discrimination ability. Our 47-mRNA metastasis-related prognostic model and nomogram might be an efficient and valuable tool for overall survival (OS) prediction and provide information for individualized treatment decisions in patients with MB.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.18632/aging.103923DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7695392PMC
November 2020
-->