Publications by authors named "Kouhei Tsumoto"

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A combination of F NMR and surface plasmon resonance for site-specific hit selection and validation of fragment molecules that bind to the ATP-binding site of a kinase.
Bioorg Med Chem 2018 May 22;26(8):1929-1938. Epub 2018 Feb 22.
Department of Bioengineering, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan; Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan. Electronic address:


Synergistic Cytotoxic Effect on Gastric Cancer Cells of an Immunotoxin Cocktail in Which Antibodies Recognize Different Epitopes on CDH17.
Monoclon Antib Immunodiagn Immunother 2018 Feb;37(1):1-11
1 Department of Quantitative Biology and Medicine, Research Center for Advanced Science and Technology, The University of Tokyo , Tokyo, Japan .

Structure of the triose-phosphate/phosphate translocator reveals the basis of substrate specificity.
Nat Plants 2017 Oct 2;3(10):825-832. Epub 2017 Oct 2.
Department of Biological Sciences, Graduate School of Science, University of Tokyo, 2-11-16 Yayoi, Bunkyo-ku, Tokyo, 113-0032, Japan.

Functional Contacts between MPER and the Anti-HIV-1 Broadly Neutralizing Antibody 4E10 Extend into the Core of the Membrane.
J Mol Biol 2017 Apr 11;429(8):1213-1226. Epub 2017 Mar 11.
Department of Bioengineering, Graduate School of Engineering, The University of Tokyo, Bunkyo-ku, Tokyo 113-8656, Japan; Laboratory of Global Healthcare, School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-Ku, Fukuoka 812-8582, Japan. Electronic address:

Peripheral Membrane Interactions Boost the Engagement by an Anti-HIV-1 Broadly Neutralizing Antibody.
J Biol Chem 2017 Mar 17;292(13):5571-5583. Epub 2017 Feb 17.
From the Biofisika Institute (Consejo Superior de Investigaciones Científicas, UPV/EHU), and Department of Biochemistry and Molecular Biology, University of the Basque Country (UPV/EHU), 48080 Bilbao, Spain and

Integrative Network Analysis Combined with Quantitative Phosphoproteomics Reveals Transforming Growth Factor-beta Receptor type-2 (TGFBR2) as a Novel Regulator of Glioblastoma Stem Cell Properties.
Mol Cell Proteomics 2016 Mar 15;15(3):1017-31. Epub 2015 Dec 15.
From the ‡Medical Proteomics Laboratory, The Institute of Medical Science, The University of Tokyo, 4-6-1, Shirokanedai, Minato-ku, Tokyo 108-8639, Japan;


Differential binding of prohibitin-2 to estrogen receptor α and to drug-resistant ERα mutants.
Biochem Biophys Res Commun 2015 Aug 4;463(4):726-31. Epub 2015 Jun 4.
Department of Chemistry and Biotechnology, School of Engineering, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan; Department of Bioengineering, School of Engineering, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8654, Japan. Electronic address:

Thermodynamics of antibody-antigen interaction revealed by mutation analysis of antibody variable regions.
J Biochem 2015 Jul 7;158(1):1-13. Epub 2015 May 7.
Department of Bioengineering, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-Ku, Tokyo 113-8656 and Medical Proteomics Laboratory, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-Ku, Tokyo 108-8639 Japan Department of Bioengineering, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-Ku, Tokyo 113-8656 and Medical Proteomics Laboratory, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-Ku, Tokyo 108-8639 Japan

Osteomodulin regulates diameter and alters shape of collagen fibrils.
Biochem Biophys Res Commun 2015 Jul 20;463(3):292-6. Epub 2015 May 20.
Department of Chemistry and Biotechnology, School of Engineering, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan; Department of Bioengineering, School of Engineering, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8654, Japan; Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan. Electronic address:

pH responsiveness of fibrous assemblies of repeat-sequence amphipathic α-helix polypeptides.
Protein Sci 2015 May 2;24(5):883-94. Epub 2015 Apr 2.
Department of Medical Genome Sciences, Graduate School of Frontier, The university of Tokyo, Kashiwanoha, Kashiwa, Chiba, Japan; Institute for Biomolecular Science, Gakushuin University, Toshima-ku, Tokyo, Japan.

The Atomic Structure of the HIV-1 gp41 Transmembrane Domain and Its Connection to the Immunogenic Membrane-proximal External Region.
J Biol Chem 2015 May 18;290(21):12999-3015. Epub 2015 Mar 18.
From the Biophysics Unit (Consejo Superior de Investigaciones Científicas, UPV/EHU) and Department of Biochemistry and Molecular Biology, University of the Basque Country (UPV/EHU), P. O. Box 644, 48080 Bilbao, Spain,

A pore-forming toxin requires a specific residue for its activity in membranes with particular physicochemical properties.
J Biol Chem 2015 Apr 10;290(17):10850-61. Epub 2015 Mar 10.
From the Department of Bioengineering, Graduate School of Engineering and Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Bunkyo-ku, Tokyo 113-8656, Japan, the Medical Proteomics Laboratory, Institute of Medical Science, The University of Tokyo, Minato-ku, 108-8639 Tokyo, Japan

Structural basis for binding of human IgG1 to its high-affinity human receptor FcγRI.
Nat Commun 2015 Apr 30;6:6866. Epub 2015 Apr 30.
1] Department of Bioengineering, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Tokyo 113-8656, Japan [2] Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa 277-8562, Japan [3] Laboratory of Medical Proteomics, Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan.


Structural basis for self-assembly of a cytolytic pore lined by protein and lipid.
Nat Commun 2015 Feb 26;6:6337. Epub 2015 Feb 26.
1] Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Bunkyo-ku, Tokyo 113-8656, Japan [2] Department of Bioengineering, School of Engineering, The University of Tokyo, Bunkyo-ku, Tokyo 113-8656, Japan [3] Laboratory of Medical Proteomics, Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan.

Preparation and characterization of anti-tissue factor single-chain variable fragment antibody for cancer diagnosis.
Cancer Sci 2014 Dec 20;105(12):1631-7. Epub 2014 Nov 20.
Division of Developmental Therapeutics, Research Center for Innovative Oncology, National Cancer Center Hospital East, Chiba, Japan; Laboratory of Cancer Biology, Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Chiba, Japan.

The staphylococcal elastin-binding protein regulates zinc-dependent growth/biofilm formation.
J Biochem 2014 Sep 29;156(3):155-62. Epub 2014 Apr 29.
Medical Proteomics Laboratory, Institute of Medical Science, The University of Tokyo, 4-6-1, Shirokanedai, Minato-ku, Tokyo 108-8639, Japan; Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo, 5-1-5, Kashiwanoha, Kashiwa, Chiba 277-8562, Japan; Division of Bacteriology, International Research Center for infectious Diseases, Institute of Medical Science, The University of Tokyo, 4-6-1, Shirokanedai, Minato-ku, Tokyo 108-8639, Japan; Section of Bacterial Pathogenesis, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8510, Japan; Department of Bioengineering, Graduate School of Engineering, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-8656, Japan; and Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-8656, Japan Medical Proteomics Laboratory, Institute of Medical Science, The University of Tokyo, 4-6-1, Shirokanedai, Minato-ku, Tokyo 108-8639, Japan; Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo, 5-1-5, Kashiwanoha, Kashiwa, Chiba 277-8562, Japan; Division of Bacteriology, International Research Center for infectious Diseases, Institute of Medical Science, The University of Tokyo, 4-6-1, Shirokanedai, Minato-ku, Tokyo 108-8639, Japan; Section of Bacterial Pathogenesis, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8510, Japan; Department of Bioengineering, Graduate School of Engineering, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-8656, Japan; and Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-8656, Japan Medical Proteomics Laboratory, Institute of Medical Science, The University of Tokyo, 4-6-1, Shirokanedai, Minato-

Protective effect of the long pentraxin PTX3 against histone-mediated endothelial cell cytotoxicity in sepsis.
Sci Signal 2014 Sep 16;7(343):ra88. Epub 2014 Sep 16.
Department of Quantitative Biology and Medicine, Research Center for Advanced Science and Technology, The University of Tokyo, Tokyo 153-8904, Japan.

Role of positions e and g in the fibrous assembly formation of an amphipathic α-helix-forming polypeptide.
Biopolymers 2014 May;102(3):260-72
Department of Medical Genome Sciences, Graduate School of Frontier Sciences, University of Tokyo, Chiba, Japan; Institute for Biomolecular Science, Gakushuin University, Tokyo, Japan.

Heme binding mechanism of structurally similar iron-regulated surface determinant near transporter domains of Staphylococcus aureus exhibiting different affinities for heme.
Biochemistry 2013 Dec 25;52(49):8866-77. Epub 2013 Nov 25.
Department of Biotechnology and ‡Agricultural Bioinformatics Research Unit, Graduate School of Agricultural and Life Sciences, The University of Tokyo , 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.

Effects of subclass change on the structural stability of chimeric, humanized, and human antibodies under thermal stress.
Protein Sci 2013 Nov 30;22(11):1542-51. Epub 2013 Sep 30.
Bio Process Research and Development Laboratories, Production Division, Kyowa Hakko Kirin Company Limited, 100-1 Hagiwara-machi, Takasaki, Gunma, 370-0013, Japan; Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo, 108-8639, Japan; Department of Medical Genome Sciences, Graduate School of Frontier Sciences, , The University of Tokyo, Kashiwa, 277-8562, Japan.

Dynamic elements govern the catalytic activity of CapE, a capsular polysaccharide-synthesizing enzyme from Staphylococcus aureus.
FEBS Lett 2013 Nov 21;587(23):3824-30. Epub 2013 Oct 21.
Medical Proteomics Laboratory, Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo 108-8639, Japan; Department of Medical Genome Sciences, School of Frontier Sciences, The University of Tokyo, Minato-ku, Tokyo 108-8639, Japan.

Secretory production of single-chain antibody (scFv) in Brevibacillus choshinensis using novel fusion partner.
Appl Microbiol Biotechnol 2013 Oct 20;97(19):8569-80. Epub 2013 Jan 20.
Applied and Molecular Microbiology, Faculty of Agriculture, Kagoshima University, 1-21-24 Korimoto, Kagoshima, 890-0065, Japan,

Bidirectional binding property of high glycine-tyrosine keratin-associated protein contributes to the mechanical strength and shape of hair.
J Struct Biol 2013 Sep 19;183(3):484-494. Epub 2013 Jun 19.
Medical Proteomics Laboratory, Institute of Medical Science, The University of Tokyo, 4-6-1, Shirokanedai, Minato-ku, Tokyo 108-8639, Japan. Electronic address:

Effects of chain length of an amphipathic polypeptide carrying the repeated amino acid sequence (LETLAKA)(n) on α-helix and fibrous assembly formation.
Biochemistry 2013 Apr 15;52(16):2810-20. Epub 2013 Apr 15.
Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8562, Japan.

Affinity improvement of a therapeutic antibody by structure-based computational design: generation of electrostatic interactions in the transition state stabilizes the antibody-antigen complex.
PLoS One 2014 27;9(1):e87099. Epub 2014 Jan 27.
Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa, Chiba, Japan ; Department of Bioengineering, Graduate School of Engineering, The University of Tokyo, Bunkyo-ku, Tokyo, Japan ; Laboratory of Medical Proteomics, Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo, Japan ; Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.

Structural basis for amino acid export by DMT superfamily transporter YddG.
Nature 2016 06 30;534(7607):417-20. Epub 2016 May 30.
Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 2-11-16 Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan.

Production and characterization of genetically modified human IL-11 variants.
Biochim Biophys Acta 2017 02 22;1861(2):205-217. Epub 2016 Nov 22.
Department of Medical Proteomics Laboratory, Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan; Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan. Electronic address:

Structural basis for broad neutralization of HIV-1 through the molecular recognition of 10E8 helical epitope at the membrane interface.
Sci Rep 2016 12 1;6:38177. Epub 2016 Dec 1.
Biophysics Unit (CSIC, UPV/EHU) and Department of Biochemistry and Molecular Biology, University of the Basque Country, P.O. Box 644, 48080 Bilbao, Spain.

Biophysical characterization of the interaction between heme and proteins responsible for heme transfer in Streptococcus pyogenes.
Biochem Biophys Res Commun 2017 11 14;493(2):1109-1114. Epub 2017 Sep 14.
Department of Bioengineering, School of Engineering, The University of Tokyo, Bunkyo-ku, Tokyo 113-8656, Japan; Medical Proteomics Laboratory, The Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo 108-8639, Japan. Electronic address:

Production and characterization of a novel site-specific-modifiable anti-OX40-receptor single-chain variable fragment for targeted drug delivery.
Biochem Biophys Res Commun 2018 02 9;496(2):614-620. Epub 2018 Jan 9.
Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, The University of Tokyo, Tokyo, Japan; Department of Advanced Medical Innovation, St. Marianna University School of Medicine, Kanagawa, Japan. Electronic address:


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