Kosuke Yusa

Kosuke Yusa

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Kosuke Yusa

Kosuke Yusa

Publications by authors named "Kosuke Yusa"

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Low rates of mutation in clinical grade human pluripotent stem cells under different culture conditions.

Nat Commun 2020 Mar 23;11(1):1528. Epub 2020 Mar 23.

The Centre for Stem Cell Biology, Department of Biomedical Science, University of Sheffield, Western Bank, Sheffield, S10 2TN, UK.

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http://dx.doi.org/10.1038/s41467-020-15271-3DOI Listing
March 2020

Genome-wide CRISPR-Cas9 screening in mammalian cells.

Methods 2019 07 26;164-165:29-35. Epub 2019 Apr 26.

Institute for Frontier Life and Medical Sciences, Kyoto University, Kyoto, Japan.

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https://linkinghub.elsevier.com/retrieve/pii/S10462023183031
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http://dx.doi.org/10.1016/j.ymeth.2019.04.015DOI Listing
July 2019

JACKS: joint analysis of CRISPR/Cas9 knockout screens.

Genome Res 2019 03 23;29(3):464-471. Epub 2019 Jan 23.

Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridgeshire, CB10 1SA, United Kingdom.

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http://dx.doi.org/10.1101/gr.238923.118DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6396427PMC
March 2019

Genome-wide CRISPR Screens in T Helper Cells Reveal Pervasive Crosstalk between Activation and Differentiation.

Cell 2019 02 10;176(4):882-896.e18. Epub 2019 Jan 10.

Wellcome Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, UK; EMBL-European Bioinformatics Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SD, UK; Theory of Condensed Matter, Cavendish Laboratory, 19 JJ Thomson Ave, Cambridge CB3 0HE, UK. Electronic address:

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http://dx.doi.org/10.1016/j.cell.2018.11.044DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6370901PMC
February 2019

Pooled extracellular receptor-ligand interaction screening using CRISPR activation.

Genome Biol 2018 11 26;19(1):205. Epub 2018 Nov 26.

Cell Surface Signalling Laboratory, Wellcome Sanger Institute, Cambridge, CB10 1SA, UK.

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https://genomebiology.biomedcentral.com/articles/10.1186/s13
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http://dx.doi.org/10.1186/s13059-018-1581-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6258485PMC
November 2018

Genome-scale identification of cellular pathways required for cell surface recognition.

Genome Res 2018 09 18;28(9):1372-1382. Epub 2018 Jun 18.

Cell Surface Signalling Laboratory, Wellcome Trust Sanger Institute, Cambridge CB10 1SA, United Kingdom.

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http://dx.doi.org/10.1101/gr.231183.117DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6120632PMC
September 2018

A CRISPR knockout screen identifies SETDB1-target retroelement silencing factors in embryonic stem cells.

Genome Res 2018 06 4;28(6):846-858. Epub 2018 May 4.

Cellular Memory Laboratory, Cluster for Pioneering Research, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.

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http://dx.doi.org/10.1101/gr.227280.117DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5991520PMC
June 2018

Author Correction: Optimised metrics for CRISPR-KO screens with second-generation gRNA libraries.

Sci Rep 2018 Apr 12;8(1):6136. Epub 2018 Apr 12.

Wellcome Trust Sanger Institute, Hinxton, Cambridge, CB10 1SA, UK.

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http://dx.doi.org/10.1038/s41598-018-24092-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5895590PMC
April 2018

Optimised metrics for CRISPR-KO screens with second-generation gRNA libraries.

Sci Rep 2017 08 7;7(1):7384. Epub 2017 Aug 7.

Wellcome Trust Sanger Institute, Hinxton, Cambridge, CB10 1SA, UK.

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http://dx.doi.org/10.1038/s41598-017-07827-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5547152PMC
August 2017

Applications of CRISPR genome editing technology in drug target identification and validation.

Expert Opin Drug Discov 2017 Jun 23;12(6):541-552. Epub 2017 Apr 23.

b Target Sciences, GlaxoSmithKline R&D , Stevenage , UK.

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http://dx.doi.org/10.1080/17460441.2017.1317244DOI Listing
June 2017

piggyBac transposon-based insertional mutagenesis in mouse haploid embryonic stem cells.

Methods Mol Biol 2015 ;1239:15-28

Breakthrough Breast Cancer Research Centre, Institute of Cancer Research, London, UK.

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http://dx.doi.org/10.1007/978-1-4939-1862-1_2DOI Listing
July 2015

piggyBac Transposon.

Authors:
Kosuke Yusa

Microbiol Spectr 2015 Apr;3(2):MDNA3-0028-2014

Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK.

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http://dx.doi.org/10.1128/microbiolspec.MDNA3-0028-2014DOI Listing
April 2015

Off-target assessment of CRISPR-Cas9 guiding RNAs in human iPS and mouse ES cells.

Genesis 2015 Feb 10;53(2):225-36. Epub 2014 Dec 10.

Wellcome Trust Sanger Institute, Hinxton, Cambridge, United Kingdom.

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http://dx.doi.org/10.1002/dvg.22835DOI Listing
February 2015

Simulation and estimation of gene number in a biological pathway using almost complete saturation mutagenesis screening of haploid mouse cells.

BMC Genomics 2014 Nov 24;15:1016. Epub 2014 Nov 24.

Department of Social and Environmental Medicine, Graduate School of Medicine, Osaka University, 2-2 Yamada-oka, Suita, Osaka 565-0871, Japan.

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http://dx.doi.org/10.1186/1471-2164-15-1016DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4301880PMC
November 2014

Removal of reprogramming transgenes improves the tissue reconstitution potential of keratinocytes generated from human induced pluripotent stem cells.

Stem Cells Transl Med 2014 Sep 14;3(9):992-1001. Epub 2014 Jul 14.

Department of Dermatology and Department of Social and Environmental Medicine, Graduate School of Medicine, Osaka University, Osaka, Japan; Department of Dermatology, Graduate School of Medicine, Tokyo Medical and Dental University, Tokyo, Japan; Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom; Department of Embryonic Stem Cell Research, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan; Department of Reproductive Biology, Center for Regenerative Medicine, National Center for Child Health and Development, Tokyo, Japan

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http://stemcellstm.alphamedpress.org/content/3/9/992.full.pd
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http://stemcellstm.alphamedpress.org/cgi/doi/10.5966/sctm.20
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http://dx.doi.org/10.5966/sctm.2013-0179DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4149296PMC
September 2014

Genome-wide recessive genetic screening in mammalian cells with a lentiviral CRISPR-guide RNA library.

Nat Biotechnol 2014 Mar 23;32(3):267-73. Epub 2013 Dec 23.

Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK.

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http://dx.doi.org/10.1038/nbt.2800DOI Listing
March 2014

Seamless genome editing in human pluripotent stem cells using custom endonuclease-based gene targeting and the piggyBac transposon.

Authors:
Kosuke Yusa

Nat Protoc 2013 Oct 26;8(10):2061-78. Epub 2013 Sep 26.

Wellcome Trust Sanger Institute, Cambridge, UK.

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http://dx.doi.org/10.1038/nprot.2013.126DOI Listing
October 2013

Enhancement of microhomology-mediated genomic rearrangements by transient loss of mouse Bloom syndrome helicase.

Genome Res 2013 Sep 1;23(9):1462-73. Epub 2013 Aug 1.

Department of Social and Environmental Medicine, Graduate School of Medicine, Osaka University, Suita, Osaka 565-0871, Japan.

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http://dx.doi.org/10.1101/gr.152744.112DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3759722PMC
September 2013

Nuclear Wave1 is required for reprogramming transcription in oocytes and for normal development.

Science 2013 Aug;341(6149):1002-5

Wellcome Trust/Cancer Research UK Gurdon Institute, The Henry Wellcome Building of Cancer and Developmental Biology, Cambridge, UK.

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http://dx.doi.org/10.1126/science.1240376DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3824084PMC
August 2013

Hyperactive piggyBac gene transfer in human cells and in vivo.

Hum Gene Ther 2012 Mar 14;23(3):311-20. Epub 2011 Dec 14.

Medical Scientist Training Program, Baylor College of Medicine, Houston, TX 77030, USA.

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http://dx.doi.org/10.1089/hum.2011.138DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3300075PMC
March 2012

Mobilization of giant piggyBac transposons in the mouse genome.

Nucleic Acids Res 2011 Dec 24;39(22):e148. Epub 2011 Sep 24.

Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK, CB10 1SA.

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https://academic.oup.com/nar/article/39/22/e148/2409515
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http://dx.doi.org/10.1093/nar/gkr764DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3239208PMC
December 2011

A homozygous mutant embryonic stem cell bank applicable for phenotype-driven genetic screening.

Nat Methods 2011 Oct 23;8(12):1071-7. Epub 2011 Oct 23.

Department of Social and Environmental Medicine, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan.

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http://dx.doi.org/10.1038/nmeth.1739DOI Listing
October 2011

Interhomolog recombination and loss of heterozygosity in wild-type and Bloom syndrome helicase (BLM)-deficient mammalian cells.

Proc Natl Acad Sci U S A 2011 Jul 5;108(29):11971-6. Epub 2011 Jul 5.

Developmental Biology Program, Memorial Sloan-Kettering Cancer Center, New York, NY 10065, USA.

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http://dx.doi.org/10.1073/pnas.1104421108DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3141969PMC
July 2011

A hyperactive piggyBac transposase for mammalian applications.

Proc Natl Acad Sci U S A 2011 Jan 4;108(4):1531-6. Epub 2011 Jan 4.

Wellcome Trust Sanger Institute, Cambridge CB10 1SA, United Kingdom.

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http://dx.doi.org/10.1073/pnas.1008322108DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3029773PMC
January 2011

Genome-wide forward genetic screens in mouse ES cells.

Methods Enzymol 2010 ;477:217-42

Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom.

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http://dx.doi.org/10.1016/S0076-6879(10)77012-9DOI Listing
December 2010

Generation of transgene-free induced pluripotent mouse stem cells by the piggyBac transposon.

Nat Methods 2009 May 31;6(5):363-9. Epub 2009 Mar 31.

Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK.

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http://dx.doi.org/10.1038/nmeth.1323DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2677165PMC
May 2009

Sleeping beauty transposase has an affinity for heterochromatin conformation.

Mol Cell Biol 2007 Mar 18;27(5):1665-76. Epub 2006 Dec 18.

Department of Social and Environmental Medicine H3, Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan.

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http://dx.doi.org/10.1128/MCB.01500-06DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1820450PMC
March 2007

Rad54 is dispensable for the ALT pathway.

Genes Cells 2006 Nov;11(11):1305-15

Department of Life Science, Graduate School of Biostudies, Kyoto University, Kyoto, Japan.

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http://dx.doi.org/10.1111/j.1365-2443.2006.01020.xDOI Listing
November 2006

Sleeping beauty transposon-based phenotypic analysis of mice: lack of Arpc3 results in defective trophoblast outgrowth.

Mol Cell Biol 2006 Aug;26(16):6185-96

Department of Social and Environmental Medicine H3, Osaka University, Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan.

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http://mcb.asm.org/cgi/doi/10.1128/MCB.00018-06
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http://dx.doi.org/10.1128/MCB.00018-06DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1592801PMC
August 2006

Bloom's syndrome gene-deficient phenotype in mouse primary cells induced by a modified tetracycline-controlled trans-silencer.

Gene 2006 Mar 4;369:80-9. Epub 2006 Jan 4.

Department of Social and Environmental Medicine, H3, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan.

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http://dx.doi.org/10.1016/j.gene.2005.10.041DOI Listing
March 2006

[Recent progress in mouse transposon research].

Tanpakushitsu Kakusan Koso 2004 Oct;49(13):2117-22

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October 2004

Genome-wide phenotype analysis in ES cells by regulated disruption of Bloom's syndrome gene.

Nature 2004 Jun;429(6994):896-9

Department of Social and Environmental Medicine, Graduate School of Medicine, Osaka University, Suita, Osaka 565-0871, Japan.

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http://dx.doi.org/10.1038/nature02646DOI Listing
June 2004

Enhancement of Sleeping Beauty transposition by CpG methylation: possible role of heterochromatin formation.

Mol Cell Biol 2004 May;24(9):4004-18

Department of Social and Environmental Medicine, Graduate School of Medicine, Osaka University, Suita, Osaka 565-0871, Japan.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC387741PMC
http://dx.doi.org/10.1128/mcb.24.9.4004-4018.2004DOI Listing
May 2004

Characterization of Sleeping Beauty transposition and its application to genetic screening in mice.

Mol Cell Biol 2003 Dec;23(24):9189-207

Collaborative Research Center for Advanced Science and Technology, Department of Social and Environmental Medicine, Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC309709PMC
http://dx.doi.org/10.1128/mcb.23.24.9189-9207.2003DOI Listing
December 2003

[New perspectives on genetically engineered mice in post-genomic era].

Tanpakushitsu Kakusan Koso 2003 Mar;48(3):232-9

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March 2003