Konstantinos Anastassiadis

Konstantinos Anastassiadis

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Konstantinos Anastassiadis

Konstantinos Anastassiadis

Publications by authors named "Konstantinos Anastassiadis"

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Prospective isolation of nonhematopoietic cells of the niche and their differential molecular interactions with HSCs.

Blood 2019 10;134(15):1214-1226

Regeneration in Hematopoiesis and Animal Models in Hematopoiesis, Institute for Immunology Medical Faculty, Technische Universität (TU) Dresden, Dresden, Germany.

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http://dx.doi.org/10.1182/blood.2019000176DOI Listing
October 2019

Aldh1b1 expression defines progenitor cells in the adult pancreas and is required for Kras-induced pancreatic cancer.

Proc Natl Acad Sci U S A 2019 10 23;116(41):20679-20688. Epub 2019 Sep 23.

Paul Langerhans Institute Dresden, Helmholtz Center Munich at the University Clinic Carl Gustav Carus of Technische Universität Dresden, German Research Center for Environmental Health, Helmholtz Zentrum München, D-85764 Neuherberg, Germany;

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http://dx.doi.org/10.1073/pnas.1901075116DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6789925PMC
October 2019

Inducible IL-7 Hyperexpression Influences Lymphocyte Homeostasis and Function and Increases Allograft Rejection.

Front Immunol 2019 10;10:742. Epub 2019 Apr 10.

Preclinical Approaches to Stem Cell Therapy/Diabetes, DFG-Center for Regenerative Therapies Dresden Cluster of Excellence, Center for Molecular and Cellular Bioengineering, Technische Universität Dresden, Dresden, Germany.

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http://dx.doi.org/10.3389/fimmu.2019.00742DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6467976PMC
April 2019

Distinct pathways affected by menin versus MLL1/MLL2 in MLL-rearranged acute myeloid leukemia.

Exp Hematol 2019 01 10;69:37-42. Epub 2018 Oct 10.

Department of Pediatrics, University of Colorado, Denver/Anschutz Medical Campus. Aurora, CO, USA; Department of Pharmacology, University of Colorado, Denver/Anschutz Medical Campus. Aurora, CO, USA. Electronic address:

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https://linkinghub.elsevier.com/retrieve/pii/S0301472X183085
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http://dx.doi.org/10.1016/j.exphem.2018.10.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6472707PMC
January 2019

A single reporter mouse line for Vika, Flp, Dre, and Cre-recombination.

Sci Rep 2018 09 27;8(1):14453. Epub 2018 Sep 27.

Stem Cell Engineering, Biotechnology Center, Technische Universität Dresden, 01307, Dresden, Germany.

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http://www.nature.com/articles/s41598-018-32802-7
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http://dx.doi.org/10.1038/s41598-018-32802-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6160450PMC
September 2018

Inductive and Selective Effects of GSK3 and MEK Inhibition on Nanog Heterogeneity in Embryonic Stem Cells.

Stem Cell Reports 2018 07 17;11(1):58-69. Epub 2018 May 17.

Department of Biosystems Science and Engineering, ETH Zurich, 4058 Basel, Switzerland; Research Unit Stem Cell Dynamics, Helmholtz Zentrum München, 85764 Neuherberg, Germany. Electronic address:

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http://dx.doi.org/10.1016/j.stemcr.2018.04.019DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6066909PMC
July 2018

A COUP-TFII Human Embryonic Stem Cell Reporter Line to Identify and Select Atrial Cardiomyocytes.

Stem Cell Reports 2017 12 22;9(6):1765-1779. Epub 2017 Nov 22.

Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, the Netherlands; Department of Applied Stem Cell Technologies, MIRA Institute, University of Twente, Twente, the Netherlands. Electronic address:

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https://linkinghub.elsevier.com/retrieve/pii/S22136711173047
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http://dx.doi.org/10.1016/j.stemcr.2017.10.024DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5785710PMC
December 2017

Single-cell mechanical phenotype is an intrinsic marker of reprogramming and differentiation along the mouse neural lineage.

Development 2017 12;144(23):4313-4321

Cellular Machines, Biotechnology Center, Center for Molecular and Cellular Bioengineering, Technische Universität Dresden, Tatzberg 47-49, Dresden 01307, Germany

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http://dev.biologists.org/lookup/doi/10.1242/dev.155218
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http://dx.doi.org/10.1242/dev.155218DOI Listing
December 2017

lncRNA Panct1 Maintains Mouse Embryonic Stem Cell Identity by Regulating TOBF1 Recruitment to Oct-Sox Sequences in Early G1.

Cell Rep 2017 Dec;21(11):3012-3021

Medical Systems Biology, UCC, Medical Faculty Carl Gustav Carus, TU Dresden, Fetscherstrasse 74, 01307 Dresden, Germany; Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany; German Cancer Research Center (DKFZ), Heidelberg and German Cancer Consortium (DKTK) partner site Dresden, 01307 Dresden, Germany; National Center for Tumor Diseases (NCT), University Hospital Carl Gustav Carus, TU Dresden, 01307 Dresden, Germany. Electronic address:

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https://linkinghub.elsevier.com/retrieve/pii/S22111247173168
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http://dx.doi.org/10.1016/j.celrep.2017.11.045DOI Listing
December 2017

The contribution of homology arms to nuclease-assisted genome engineering.

Nucleic Acids Res 2017 Jul;45(13):8105-8115

Genomics, Biotechnology Center, Technische Universität Dresden, BioInnovationsZentrum, Tatzberg 47, Dresden 01307, Germany.

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http://dx.doi.org/10.1093/nar/gkx497DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5570031PMC
July 2017

, encoding a histone 3 lysine 4 methyltransferase, is a maternal effect gene required for the oogenic gene expression program.

Development 2017 07 15;144(14):2606-2617. Epub 2017 Jun 15.

Genomics, Biotechnology Center, Technische Universität Dresden, BioInnovationsZentrum, Tatzberg 47, Dresden 01307, Germany

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http://dx.doi.org/10.1242/dev.143347DOI Listing
July 2017

MLL2, Not MLL1, Plays a Major Role in Sustaining MLL-Rearranged Acute Myeloid Leukemia.

Cancer Cell 2017 06;31(6):755-770.e6

Department of Pediatrics, Section of Hematology/Oncology/BMT, University of Colorado, Denver/Anschutz Medical Campus, Aurora, CO 80045, USA; Department of Pharmacology, University of Colorado, Denver/Anschutz Medical Campus, Aurora, CO 80045, USA. Electronic address:

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http://dx.doi.org/10.1016/j.ccell.2017.05.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5598468PMC
June 2017

TDRD6 mediates early steps of spliceosome maturation in primary spermatocytes.

PLoS Genet 2017 03 6;13(3):e1006660. Epub 2017 Mar 6.

Institute of Physiological Chemistry, Medical Faculty Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany.

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http://dx.doi.org/10.1371/journal.pgen.1006660DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5358835PMC
March 2017

RAC-tagging: Recombineering And Cas9-assisted targeting for protein tagging and conditional analyses.

Sci Rep 2016 05 24;6:25529. Epub 2016 May 24.

Genomics, Biotechnology Center, Technische Universität Dresden, BioInnovationsZentrum, Tatzberg 47, Dresden 01307, Germany.

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http://dx.doi.org/10.1038/srep25529DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4877586PMC
May 2016

Retinal Organoids from Pluripotent Stem Cells Efficiently Recapitulate Retinogenesis.

Stem Cell Reports 2016 Apr 31;6(4):525-538. Epub 2016 Mar 31.

German Center for Neurodegenerative Diseases (DZNE) Dresden, 01307 Dresden, Germany; CRTD - Center for Regenerative Therapies Dresden, Technische Universität Dresden, 01307 Dresden, Germany. Electronic address:

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http://dx.doi.org/10.1016/j.stemcr.2016.03.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4834051PMC
April 2016

Thy-1/β3 Integrin Interaction-Induced Apoptosis of Dermal Fibroblasts Is Mediated by Up-Regulation of FasL Expression.

J Invest Dermatol 2016 Feb 18;136(2):526-529. Epub 2015 Nov 18.

Department of Dermatology, Venereology and Allergology of Medical Faculty of Leipzig University, Leipzig, Germany. Electronic address:

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http://dx.doi.org/10.1016/j.jid.2015.10.063DOI Listing
February 2016

Network plasticity of pluripotency transcription factors in embryonic stem cells.

Nat Cell Biol 2015 Oct 21;17(10):1235-46. Epub 2015 Sep 21.

Research Unit Stem Cell Dynamics, Helmholtz Zentrum München-German Research Center for Environmental Health, Ingolstädter Landstr. 1, 85764 Neuherberg, Germany.

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http://dx.doi.org/10.1038/ncb3237DOI Listing
October 2015

Breast carcinoma cells modulate the chemoattractive activity of human bone marrow-derived mesenchymal stromal cells by interfering with CXCL12.

Int J Cancer 2015 Jan 20;136(1):44-54. Epub 2014 May 20.

Division of Hematology, Oncology and Stem Cell Transplantation, Department of Medicine I, University Hospital Carl Gustav Carus, Technische Universiät Dresden, Germany.

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http://dx.doi.org/10.1002/ijc.28960DOI Listing
January 2015

Cellular reporter systems for high-throughput screening of interactions between bioactive matrices and human mesenchymal stromal cells.

Tissue Eng Part C Methods 2014 Oct 25;20(10):828-37. Epub 2014 Mar 25.

1 Medical Clinic and Policlinic I, University Hospital Dresden , Technische Universität, Dresden, Germany .

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http://dx.doi.org/10.1089/ten.TEC.2013.0590DOI Listing
October 2014

Nanog induces hyperplasia without initiating tumors.

Stem Cell Res 2014 Sep 10;13(2):300-15. Epub 2014 Aug 10.

Department of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine, Röntgenstrasse 20, 48149 Münster, Germany.

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http://dx.doi.org/10.1016/j.scr.2014.08.001DOI Listing
September 2014

Gene targeting and site-specific recombination in mouse ES cells.

Methods Enzymol 2013 ;533:133-55

Center for Regenerative Therapies, Technische Universität Dresden, BioInnovationZentrum, Dresden, Germany. Electronic address:

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http://dx.doi.org/10.1016/B978-0-12-420067-8.00009-XDOI Listing
August 2014

The H3K4 methyltransferase Setd1a is first required at the epiblast stage, whereas Setd1b becomes essential after gastrulation.

Development 2014 Mar;141(5):1022-35

Stem Cell Engineering, Technische Universität Dresden, BioInnovationsZentrum, Tatzberg 47, Dresden 01307, Germany.

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http://dx.doi.org/10.1242/dev.098152DOI Listing
March 2014

Mll2 is required for H3K4 trimethylation on bivalent promoters in embryonic stem cells, whereas Mll1 is redundant.

Development 2014 Feb 14;141(3):526-37. Epub 2014 Jan 14.

Department of Molecular Biology, Faculty of Science, Nijmegen Centre for Molecular Life Sciences (NCMLS), Radboud University, PO Box 9101, 6500 HB Nijmegen, The Netherlands.

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http://dx.doi.org/10.1242/dev.102681DOI Listing
February 2014

Generation of inducible immortalized dendritic cells with proper immune function in vitro and in vivo.

PLoS One 2013 23;8(4):e62621. Epub 2013 Apr 23.

Department of Pediatrics, University Clinic Carl Gustav Carus, Technische Universitaet Dresden, Dresden, Germany.

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0062621PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3633827PMC
November 2013

Tightly anchored tissue-mimetic matrices as instructive stem cell microenvironments.

Nat Methods 2013 Aug 23;10(8):788-94. Epub 2013 Jun 23.

Leibniz Institute of Polymer Research Dresden, Max Bergmann Center of Biomaterials, Dresden, Germany.

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http://dx.doi.org/10.1038/nmeth.2523DOI Listing
August 2013

A localized Wnt signal orients asymmetric stem cell division in vitro.

Science 2013 Mar;339(6126):1445-8

Department of Developmental Biology, Howard Hughes Medical Institute, Institute for Stem Cell Biology and Regenerative Medicine, Stanford University, 265 Campus Drive, Stanford, CA 94305, USA.

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http://dx.doi.org/10.1126/science.1231077DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3966430PMC
March 2013

Transposon mediated BAC transgenesis via pronuclear injection of mouse zygotes.

Genesis 2013 Feb 25;51(2):135-41. Epub 2013 Jan 25.

Stem Cell Engineering, Biotechnology Center, Technische Universitaet Dresden, Dresden, Germany.

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http://dx.doi.org/10.1002/dvg.22362DOI Listing
February 2013

Transposon-mediated BAC transgenesis in human ES cells.

Nucleic Acids Res 2012 Oct 30;40(19):e150. Epub 2012 Jun 30.

Stem Cell Engineering, BioInnovationsZentrum, Technische Universitaet Dresden, Dresden 01307, Germany.

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http://dx.doi.org/10.1093/nar/gks643DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3479164PMC
October 2012

The mammalian gene function resource: the International Knockout Mouse Consortium.

Mamm Genome 2012 Oct 12;23(9-10):580-6. Epub 2012 Sep 12.

The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1HH, UK.

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http://dx.doi.org/10.1007/s00335-012-9422-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3463800PMC
October 2012

Meiotic homologue alignment and its quality surveillance are controlled by mouse HORMAD1.

Nat Cell Biol 2011 May 10;13(5):599-610. Epub 2011 Apr 10.

Institute of Physiological Chemistry, Technische Universität Dresden, Fiedlerstrasse 42, 01307 Dresden, Germany.

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http://dx.doi.org/10.1038/ncb2213DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3087846PMC
May 2011

Recombineering BAC transgenes for protein tagging.

Methods 2011 Feb 22;53(2):113-9. Epub 2010 Sep 22.

Genomics, BioInnovationsZentrum, Technische Universitaet Dresden, Am Tatzberg 47, 01307 Dresden, Germany.

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http://dx.doi.org/10.1016/j.ymeth.2010.09.003DOI Listing
February 2011

PTBP1 is required for embryonic development before gastrulation.

PLoS One 2011 Feb 17;6(2):e16992. Epub 2011 Feb 17.

Molecular Diabetology, Paul Langerhans Institute Dresden, School of Medicine and University Clinic Carl Gustav Carus, Dresden University of Technology, Dresden, Germany.

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0016992PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3040740PMC
February 2011

A recombineering pipeline to make conditional targeting constructs.

Methods Enzymol 2010 ;477:125-44

Genomics, BioInnovationsZentrum, Technische Universitaet Dresden, Dresden, Germany.

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http://dx.doi.org/10.1016/S0076-6879(10)77008-7DOI Listing
December 2010

Dually inducible TetON systems for tissue-specific conditional gene expression in zebrafish.

Proc Natl Acad Sci U S A 2010 Nov 1;107(46):19933-8. Epub 2010 Nov 1.

Biotechnology Center, Technische Universität Dresden, 01307 Dresden, Germany.

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http://www.pnas.org/cgi/doi/10.1073/pnas.1007799107
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http://dx.doi.org/10.1073/pnas.1007799107DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2993373PMC
November 2010

Tamoxifen-independent recombination in the RIP-CreER mouse.

PLoS One 2010 Oct 22;5(10):e13533. Epub 2010 Oct 22.

Molecular Diabetology, Paul Langerhans Institute Dresden, School of Medicine and University Clinic Carl Gustav Carus, Dresden University of Technology, Dresden, Germany.

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0013533PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2965077PMC
October 2010

An improved Flp deleter mouse in C57Bl/6 based on Flpo recombinase.

Genesis 2010 Aug;48(8):512-20

Genomics, BioInnovationsZentrum, Technische Universität Dresden, Am Tatzberg 47, Dresden, Germany.

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http://dx.doi.org/10.1002/dvg.20641DOI Listing
August 2010

MLL2 is required in oocytes for bulk histone 3 lysine 4 trimethylation and transcriptional silencing.

PLoS Biol 2010 Aug 17;8(8). Epub 2010 Aug 17.

Department of Pathology and Immunology, Baylor College of Medicine, Houston, Texas, United States of America.

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http://dx.doi.org/10.1371/journal.pbio.1000453DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2923083PMC
August 2010

Precise conditional immortalization of mouse cells using tetracycline-regulated SV40 large T-antigen.

Genesis 2010 Apr;48(4):220-32

Center for Regenerative Therapies Dresden, BioInnovationsZentrum, Technische Universität Dresden, Dresden, Germany.

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http://dx.doi.org/10.1002/dvg.20605DOI Listing
April 2010

Dre recombinase, like Cre, is a highly efficient site-specific recombinase in E. coli, mammalian cells and mice.

Dis Model Mech 2009 Sep-Oct;2(9-10):508-15. Epub 2009 Aug 19.

Center for Regenerative Therapies Dresden, BioInnovationsZentrum Technische Universitaet Dresden, Am Tatzberg 47, 01307 Dresden, Germany.

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http://dx.doi.org/10.1242/dmm.003087DOI Listing
January 2010

Doxycycline-dependent photoactivated gene expression in eukaryotic systems.

Nat Methods 2009 Jul 7;6(7):527-31. Epub 2009 Jun 7.

Max Planck Institute of Neurobiology, Munich-Martinsried, Germany.

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http://dx.doi.org/10.1038/nmeth.1340DOI Listing
July 2009

Bacterial artificial chromosome transgenesis through pronuclear injection of fertilized mouse oocytes.

Methods Mol Biol 2008 ;415:83-100

Mount Sinai Hospital, Samuel Lunenfeld Research Institute, Toronto, Ontario, Canada.

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http://dx.doi.org/10.1007/978-1-59745-570-1_5DOI Listing
June 2008

Engineering embryonic stem cells with recombinase systems.

Methods Enzymol 2006 ;420:100-36

Department for Molecular Hematology, University of Frankfurt Medical School, Frankfurt am Main, Germany.

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http://dx.doi.org/10.1016/S0076-6879(06)20007-7DOI Listing
August 2007

Increased apoptosis and skewed differentiation in mouse embryonic stem cells lacking the histone methyltransferase Mll2.

Mol Biol Cell 2007 Jun 11;18(6):2356-66. Epub 2007 Apr 11.

Genomics Group, BioInnovations Zentrum, Technische Universität Dresden, 01307 Dresden, Germany.

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http://dx.doi.org/10.1091/mbc.e06-11-1060DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1877088PMC
June 2007

Multiple epigenetic maintenance factors implicated by the loss of Mll2 in mouse development.

Development 2006 Apr 15;133(8):1423-32. Epub 2006 Mar 15.

Genomics, BioInnovationsZentrum, Dresden University of Technology, Am Tatzberg 47, Dresden 01307, Germany.

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http://dx.doi.org/10.1242/dev.02302DOI Listing
April 2006

Current issues in mouse genome engineering.

Nat Genet 2005 Nov;37(11):1187-93

Genomics, BioInnovationsZentrum, Dresden University of Technology, Am Tatzberg 47, 01307, Dresden, Germany.

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http://www.nature.com/articles/ng1668
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http://dx.doi.org/10.1038/ng1668DOI Listing
November 2005

A reliable lacZ expression reporter cassette for multipurpose, knockout-first alleles.

Genesis 2004 Mar;38(3):151-8

Biotec, Technische Universität Dresden, c/o Max Planck Institute of Molecular cell Biology and Genetics, Dresden, Germany.

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http://doi.wiley.com/10.1002/gene.20012
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http://dx.doi.org/10.1002/gene.20012DOI Listing
March 2004

A predictable ligand regulated expression strategy for stably integrated transgenes in mammalian cells in culture.

Gene 2002 Oct;298(2):159-72

BIOTEC, TU Dresden, c/o Max-Planck-Institut für Molekulare Zellbiologie und Genetik MPI-CBG, Pfotenhauerstrasse 108, 01307 Dresden, Germany.

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http://dx.doi.org/10.1016/s0378-1119(02)00979-4DOI Listing
October 2002