Publications by authors named "Kohei Yamamoto"

61 Publications

Concurrent evaluation of salivary and urinary α-amylase activity following prolonged exercise with or without carbohydrate solution in aerobically active men.

Neuro Endocrinol Lett 2021 Aug 28;42(4):265-276. Epub 2021 Aug 28.

Research Center in Sports Medicine and Science, Department of Physical Education, International Pacific University, Okayama 709-0863, Japan.

Objective: The purpose of this study was to determine the effects of 2-h moderately prolonged exercise with carbohydrate intake or water placebo on salivary and urinary α-amylase isoenzyme activity in trained men.

Materials And Methods: Eleven aerobically fit men participated in this study. On two different occasions, participants performed 2-h cycling corresponding to a constant power output at 60% peak oxygen uptake. The study design involved a random order, placebo-controlled and cross-over assignment. Participants consumed either 6.2% carbohydrate solution or water placebo every twenty minutes thereafter (2 ml/kg body mass) over 2-h endurance exercise. Unstimulated whole salivary samples were collected using the passive drooling method at the 10-min period before and after exercise for the quantification of salivary α-amylase, immunoglobulin A (IgA) and total protein. Two-hour urinary samples were obtained at three time points before (-2-0h), immediately (0-2 h) after and 24-26 h after exercise for the analysis of α-amylase isoenzyme activity (pancreas- and saliva-derived types).

Results: The activity of α-amylase in saliva and urine was significantly increased in connect with salivary total protein concentration immediately after moderately long-lasting exercise, but salivary IgA concentration was not statistically significant with or without exogenous carbohydrate intake.

Conclusion: These findings suggest that 2-h moderate exercise appears to lead to the enhanced α-amylase activity in saliva and urine regardless of exogenous carbohydrate availability, demonstrating enhanced mucosal immune defense.
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August 2021

Functional roles of fish collagen peptides on bone regeneration.

Dent Mater J 2021 Jul 31. Epub 2021 Jul 31.

Department of Periodontology and Endodontology, Nagasaki University Graduate School of Biomedical Sciences.

Fish collagen peptides (FCP) derived from the skin, bones and scales are commercially used as a functional food or dietary supplement for hypertension and diabetes. However, there is limited evidence on the effects of FCP on the osteoblast function in contrast to evidence of the effects on wound healing, diabetes and bone regeneration, which have been obtained from animal studies. In this narrative review, we expound on the availability of FCP by basic research using osteoblasts. Low-concentration FCP upregulates the expression of osteoblast proliferation, differentiation and collagen modifying enzyme-related genes. Furthermore, it could accelerate matrix mineralization. FCP may have potential utility as a biomaterial to improve collagen quality and promote mineralization through the mitogen-activated protein kinase and Smad cascades. However, there are few clinical studies on bone regeneration in human subjects. It is desirable to be applied clinically through clinical study as soon as possible, based on the results from basic research.
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http://dx.doi.org/10.4012/dmj.2020-446DOI Listing
July 2021

The role of toxic planocerid flatworm larvae on tetrodotoxin accumulation in marine bivalves.

Aquat Toxicol 2021 Aug 6;237:105908. Epub 2021 Jul 6.

Department of Marine Science and Resources, Nihon University, Fujisawa, Kanagawa 252-0880, Japan. Electronic address:

Tetrodotoxin (TTX), also known as pufferfish toxin, has been detected in marine edible bivalves worldwide. In this study, several bivalve species, Azumapecten farreri subsp. akazara, Patinopecten yessoensis and Mytilus galloprovincialis, collected from the Pacific side of the northern Japanese Islands, were studied for the accumulation of TTX in the presence of toxic planocerid larvae. LC-MS/MS analysis demonstrated that TTX was detected only in the midgut gland of A. farreri subsp. akazara. Toxic flatworm-specific PCR and direct sequencing of the amplicons showed that the DNA fragments of the Planocera multitentaculata COI gene were detected in the gut contents of the toxified bivalves. The planocerid larvae were also detected in the environmental seawaters. Toxification experiments in the aquarium demonstrated that the mussel M. galloprovincialis was also toxified by feeding on the toxic flatworm larvae. These results suggest that the source of TTX accumulation in edible bivalves is toxic flatworm larvae.
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http://dx.doi.org/10.1016/j.aquatox.2021.105908DOI Listing
August 2021

Constraints on Cosmic Strings Using Data from the Third Advanced LIGO-Virgo Observing Run.

Phys Rev Lett 2021 Jun;126(24):241102

SUPA, University of Strathclyde, Glasgow G1 1XQ, United Kingdom.

We search for gravitational-wave signals produced by cosmic strings in the Advanced LIGO and Virgo full O3 dataset. Search results are presented for gravitational waves produced by cosmic string loop features such as cusps, kinks, and, for the first time, kink-kink collisions. A template-based search for short-duration transient signals does not yield a detection. We also use the stochastic gravitational-wave background energy density upper limits derived from the O3 data to constrain the cosmic string tension Gμ as a function of the number of kinks, or the number of cusps, for two cosmic string loop distribution models. Additionally, we develop and test a third model that interpolates between these two models. Our results improve upon the previous LIGO-Virgo constraints on Gμ by 1 to 2 orders of magnitude depending on the model that is tested. In particular, for the one-loop distribution model, we set the most competitive constraints to date: Gμ≲4×10^{-15}. In the case of cosmic strings formed at the end of inflation in the context of grand unified theories, these results challenge simple inflationary models.
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http://dx.doi.org/10.1103/PhysRevLett.126.241102DOI Listing
June 2021

Insights into Microscopic Crystal Growth Dynamics of CHNHPbI under a Laser Deposition Process Revealed by X-ray Diffraction.

ACS Appl Mater Interfaces 2021 May 7;13(19):22559-22566. Epub 2021 May 7.

National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan.

The process dynamics for the vacuum deposition of methylammonium lead iodide (MAPbI) perovskite was analyzed by X-ray diffraction using synchrotron radiation. MAPbI was fabricated by alternatingly supplying PbI and methylammonium iodide a laser deposition system installed at the synchrotron beamline BL46XU at SPring-8, and crystallization analysis was conducted. Microscopic insights into the crystallization were obtained, including observation of Laue oscillation during the PbI growth and octahedral unit (PbI) rotation during the transformation into perovskite. On the basis of this analysis, conditions that favor the construction of atomically flat MAPbI perovskite films were deduced.
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http://dx.doi.org/10.1021/acsami.1c04488DOI Listing
May 2021

Detecting prediction limit of marked point processes using constrained random shuffle surrogate data.

Chaos 2021 Jan;31(1):013122

Department of Management Science, Graduate School of Engineering, Tokyo University of Science, 6-3-1 Niijuku, Katsushika, Tokyo 125-8585, Japan.

Marked point processes refer to time series of discrete events with additional information about the events. Seismic activities, neural activities, and price movements in financial markets are typical examples of marked point process data. In this paper, we propose a method for investigating the prediction limits of marked point process data, where random shuffle surrogate data with time window constraints are proposed and utilized to estimate the prediction limits. We applied the proposed method to the marked point process data obtained from several dynamical systems and investigated the relationship between the largest Lyapunov exponent and the prediction limit estimated by the proposed method. The results revealed a positive correlation between the reciprocal of the estimated prediction limit and the largest Lyapunov exponent of the underlying dynamical systems in marked point processes.
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http://dx.doi.org/10.1063/5.0005267DOI Listing
January 2021

[Marginal zone lymphoma-like primary bone marrow lymphoma with long-term pancytopenia preceding diagnosis].

Rinsho Ketsueki 2020 ;61(10):1469-1475

Department of Hematology, Medical Hospital, Tokyo Medical and Dental University.

A 45-year-old man initially diagnosed with aplastic anemia had been receiving treatment for >4 years when he visited our hospital for a detailed examination. On admission, bone marrow (BM) aspiration showed erythroid dysplasia and chromosomal abnormalities, including trisomy 3 in 1/20 cells. After 3 months of observation, BM aspiration showed the involvement of 5% abnormal lymphocytes, and flow cytometry revealed a monoclonal B-cell phenotype. After a further 5 months of observation, his blood test showed a sudden elevation in white blood cell (WBC) count and the presence of villous lymphocytes. Fluorodeoxyglucose-positron emission tomography (FDG-PET) only revealed strong uptake by systemic BM, and BM aspiration showed the involvement of 76.4% abnormal lymphocytes, which were positive for CD19 and dim CD11c; negative for CD25, CD103, cyclin D1, and BRAF-V600E; and exhibited light chain restriction. The patient was diagnosed with marginal zone lymphoma-like primary bone marrow (BM) lymphoma. Treatment with R-CHOP and R-cladribine failed. He then underwent an allogeneic peripheral blood stem cell transplantation from a human leucocyte antigen (HLA)-identical sibling, and he has since remained in good health and without relapse for 9 years. Further clinical and biological analyses are necessary to establish an optimal treatment strategy for this disease.
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http://dx.doi.org/10.11406/rinketsu.61.1469DOI Listing
January 2021

Favorable effect of sodium-glucose cotransporter 2 inhibitor, dapagliflozin, on non-alcoholic fatty liver disease compared with pioglitazone.

J Diabetes Investig 2021 Jul 2;12(7):1272-1277. Epub 2020 Dec 2.

Department of Rheumatology, Endocrinology and Nephrology, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, Sapporo, Japan.

Aims/introduction: Sodium-glucose cotransporter 2 inhibitors, as well as thiazolidines, suppress nonalcoholic fatty liver disease (NAFLD); however, few comparative studies have been reported. Dapagliflozin has shown non-inferiority compared with pioglitazone for glycemic control, and superiority regarding weight reduction in patients with type 2 diabetes. We carried out a secondary analysis for the favorable effects of sodium-glucose cotransporter inhibitors for NAFLD.

Materials And Methods: In this multicenter, open-label, prospective, randomized, parallel-group comparison trial, patients taking pioglitazone for ≥12 weeks were randomly switched to dapagliflozin or continued pioglitazone for a further 24 weeks. The fatty liver index (FLI), consisting of body mass index, triglycerides, waist circumference and γ-glutamyl transpeptidase, was used for the evaluation of NAFLD.

Results: A total of 53 participants with NAFLD (27 dapagliflozin; 26 pioglitazone) were included in this analysis. FLI decreased significantly in the dapagliflozin group (48.7 ± 23.4 to 42.1 ± 23.9) compared with the pioglitazone group (49.0 ± 26.1 to 51.1 ± 25.8; P < 0.01). Multiple linear regression analysis showed that the changes in FLI had a significantly positive correlation with changes in glycated hemoglobin (P = 0.03) and insulin level (P < 0.01) in the dapagliflozin group.

Conclusion: Dapagliflozin might be more beneficial than pioglitazone in patients with NAFLD. Improvements in FLI would be closely related to glycemic control.
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http://dx.doi.org/10.1111/jdi.13457DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8264405PMC
July 2021

Prospects for observing and localizing gravitational-wave transients with Advanced LIGO, Advanced Virgo and KAGRA.

Living Rev Relativ 2020 28;23(1). Epub 2020 Sep 28.

LIGO, California Institute of Technology, Pasadena, CA 91125 USA.

We present our current best estimate of the plausible observing scenarios for the Advanced LIGO, Advanced Virgo and KAGRA gravitational-wave detectors over the next several years, with the intention of providing information to facilitate planning for multi-messenger astronomy with gravitational waves. We estimate the sensitivity of the network to transient gravitational-wave signals for the third (O3), fourth (O4) and fifth observing (O5) runs, including the planned upgrades of the Advanced LIGO and Advanced Virgo detectors. We study the capability of the network to determine the sky location of the source for gravitational-wave signals from the inspiral of binary systems of compact objects, that is binary neutron star, neutron star-black hole, and binary black hole systems. The ability to localize the sources is given as a sky-area probability, luminosity distance, and comoving volume. The median sky localization area (90% credible region) is expected to be a few hundreds of square degrees for all types of binary systems during O3 with the Advanced LIGO and Virgo (HLV) network. The median sky localization area will improve to a few tens of square degrees during O4 with the Advanced LIGO, Virgo, and KAGRA (HLVK) network. During O3, the median localization volume (90% credible region) is expected to be on the order of for binary neutron star, neutron star-black hole, and binary black hole systems, respectively. The localization volume in O4 is expected to be about a factor two smaller than in O3. We predict a detection count of ( ) for binary neutron star mergers, of ( ) for neutron star-black hole mergers, and ( ) for binary black hole mergers in a one-calendar-year observing run of the HLV network during O3 (HLVK network during O4). We evaluate sensitivity and localization expectations for unmodeled signal searches, including the search for intermediate mass black hole binary mergers.
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http://dx.doi.org/10.1007/s41114-020-00026-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7520625PMC
September 2020

Single-Element Dual-Interferometer for Precision Inertial Sensing.

Sensors (Basel) 2020 Sep 3;20(17). Epub 2020 Sep 3.

Max-Planck-Institut für Gravitationsphysik (Albert-Einstein-Institut) and Institut für Gravitationsphysik, Leibniz Universität Hannover, Callinstrasse 38, D-30167 Hannover, Germany.

Tracking moving masses in several degrees of freedom with high precision and large dynamic range is a central aspect in many current and future gravitational physics experiments. Laser interferometers have been established as one of the tools of choice for such measurement schemes. Using sinusoidal phase modulation homodyne interferometry allows a drastic reduction of the complexity of the optical setup, a key limitation of multi-channel interferometry. By shifting the complexity of the setup to the signal processing stage, these methods enable devices with a size and weight not feasible using conventional techniques. In this paper we present the design of a novel sensor topology based on deep frequency modulation interferometry: the self-referenced single-element dual-interferometer (SEDI) inertial sensor, which takes simplification one step further by accommodating two interferometers in one optic. Using a combination of computer models and analytical methods we show that an inertial sensor with sub-picometer precision for frequencies above 10 mHz, in a package of a few cubic inches, seems feasible with our approach. Moreover we show that by combining two of these devices it is possible to reach sub-picometer precision down to 2 mHz. In combination with the given compactness, this makes the SEDI sensor a promising approach for applications in high precision inertial sensing for both next-generation space-based gravity missions employing drag-free control, and ground-based experiments employing inertial isolation systems with optical readout.
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http://dx.doi.org/10.3390/s20174986DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7506752PMC
September 2020

High amplification of PVT1 and MYC predict favorable prognosis in early ovarian carcinoma.

Pathol Res Pract 2020 Nov 16;216(11):153175. Epub 2020 Aug 16.

Department of Comprehensive Pathology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Japan.

Introduction: The objective of this study was to evaluate the status of MYC and PVT1, which are frequently amplified in malignant tumors, and to assess their biological features according to histological subtypes in early-stage epithelial ovarian cancer (EOC).

Methods: Formalin-fixed and paraffin-embedded (FFPE) samples of 64 EOC tissues in International Federation of Gynecology and Obstetrics stages I-II and 20 normal ovarian tissues were analyzed for copy number and mRNA expression of MYC and PVT1 by qPCR and for MYC protein expression by immunohistochemistry. MYC protein expression was assessed by western blotting in a PVT1 siRNA-transfected ovarian cancer cell line. MYC and PVT1 was assessed as a prognostic factor using Kaplan-Meier analysis. The median follow-up period was 49.9 months and 17 cases in 64 of EOC recurred during follow-up.

Results: Copy number variations showed significantly higher MYC and PVT1 in EOC than in normal ovaries. The copy number of PVT1 was significantly higher in serous carcinoma than in the other histological types. The mRNA of MYC and PVT1 was also higher in cancer tissues and showed a strong correlation in all histological subtypes. Immunohistochemistry revealed a positive association between the phosphorylated MYC (pMYC) index and high expression of proliferation markers, such as Ki-67 index, and a negative correlation between pMYC protein and the PVT1 copy number. Knockdown of PVT1 in ovarian cancer cell lines resulted in upregulation of MYC protein and pMYC. Kaplan-Meier survival analysis showed that low copy numbers of both MYC and PVT1 were associated with a statistically significantly poor prognosis.

Conclusion: Expression of pMYC and the Ki-67 index were affected by the PVT1 copy number but not mRNA. A high PVT1 copy number in FFPE samples might suggest favorable prognosis in early ovarian cancers.
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http://dx.doi.org/10.1016/j.prp.2020.153175DOI Listing
November 2020

Renal TNFα activates the WNK phosphorylation cascade and contributes to salt-sensitive hypertension in chronic kidney disease.

Kidney Int 2020 04 11;97(4):713-727. Epub 2020 Feb 11.

Department of Nephrology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.

The inappropriate over-activation of the with-no-lysine kinase (WNK)-STE20/SPS1-related proline/alanine-rich kinase (SPAK)-sodium chloride cotransporter (NCC) phosphorylation cascade increases sodium reabsorption in distal kidney nephrons, resulting in salt-sensitive hypertension. Although chronic kidney disease (CKD) is a common cause of salt-sensitive hypertension, the involvement of the WNK phosphorylation cascade is unknown. Moreover, the effect of immune systems on WNK kinases has not been investigated despite the fact that immune systems are important for salt sensitivity. Here we demonstrate that the protein abundance of WNK1, but not of WNK4, was increased at the distal convoluted tubules in the aristolochic acid nephropathy mouse model of CKD. Accordingly, the phosphorylation of both SPAK and NCC was also increased. Moreover, a high-salt diet did not adequately suppress activation of the WNK1-SPAK-NCC phosphorylation cascade in this model, leading to salt-sensitive hypertension. WNK1 also was increased in adenine nephropathy, but not in subtotal nephrectomy, models of CKD. By comparing the transcripts of these three models focusing on immune systems, we hypothesized that tumor necrosis factor (TNF)-α regulates WNK1 protein expression. In fact, TNF-α increased WNK1 protein expression in cultured renal tubular cells by reducing the transcription and protein levels of NEDD4-2 E3-ligase, which degrades WNK1 protein. Furthermore, the TNF-α inhibitor etanercept reversed the reduction of NEDD4-2 expression and upregulation of the WNK1-SPAK-NCC phosphorylation cascade in distal convoluted tubules in vivo in the aristolochic acid nephropathy model. Thus, salt-sensitive hypertension is induced in CKD via activation of the renal WNK1- SPAK-NCC phosphorylation cascade by TNF-α, reflecting a link with the immune system.
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http://dx.doi.org/10.1016/j.kint.2019.11.021DOI Listing
April 2020

Taxonomic study of Endogonaceae in the Japanese islands: New species of , and , gen. nov.

Mycologia 2020 Mar-Apr;112(2):309-328. Epub 2020 Jan 22.

Department of Bioscience and Food Production Science, Interdisciplinary Graduate School of Science and Technology, Shinshu University, 8304, Minami-minowa, Nagano 399-4598, Japan.

Species of Endogonaceae (Endogonales, Mucoromycotina) are characterized by the formation of relatively large sporocarps and zygosporangia. Numerous species in this family remain undescribed or have unclear phylogenetic positions. In Asia specifically, the species diversity of this family is almost completely unknown. However, many mycobionts of bryophytes belonging to several novel clades in Endogonaceae have recently been identified phylogenetically. Therefore, establishing a robust taxonomic system for this family is essential. We obtained numerous sporocarps of undescribed Endogonaceae-like species from the Japanese islands. Morphological observation and multilocus phylogenetic analysis of nuc 18S rDNA (18S), nuc 28S rDNA (28S), and portions of two nuclear protein-coding regions-translation elongation factor 1-alpha () and RNA polymerase II large subunit ()-from these species resulted in the description of one new species each of and and two new species of , gen. nov. Because is characterized by purplish sporocarps and red-wine-colored chlamydospores up to 700 μm in diameter, we emended the definition of Endogonaceae.
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http://dx.doi.org/10.1080/00275514.2019.1689092DOI Listing
March 2021

MUSIC-based Non-contact Heart Rate Estimation with Adaptive Window Size Setting.

Annu Int Conf IEEE Eng Med Biol Soc 2019 Jul;2019:6073-6076

Continuous HR (Heart Rate) monitoring enables the stress estimation in daily life. A Doppler sensor could be a key device to facilitate the non-contact HR estimation. As one of the Doppler sensor-based HR estimation methods, we have previously proposed a MUSIC (MUltiple SIgnal Classification)-based HR estimation method. MUSIC is the algorithm widely used as a tool to estimate DOA (Direction of Arrival). In our previous method, MUSIC spectrum is calculated in each sliding window, and then HR is estimated by the maximum peak detection over the MUSIC spectrum. However, when HR changes largely within the window, several peaks due to heartbeats appear over the MUSIC spectrum, which might cause the incorrect peak detection. Hence, an adaptive window is required so that only one peak appears. In this paper, we propose a MUSIC-based HR estimation method with an adaptive window size setting. When several peaks due to heartbeats appear over the MUSIC spectrum, our proposed method shortens the time window and re-calculates the MUSIC spectrum, which is repeated until only one peak appears. The experimental results showed that our method outperformed not only our previous one but also the other existing MUSIC-based HR estimation one in terms of the estimation accuracy of the HR, the stress indexes CVI (Cardiac Vagal Index) and CSI (Cardiac Sympathetic Index).
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http://dx.doi.org/10.1109/EMBC.2019.8857076DOI Listing
July 2019

Novel CA-CFAR Approach for Improvement of Doppler Sensor-based Heart Rate Variability Estimation.

Annu Int Conf IEEE Eng Med Biol Soc 2019 Jul;2019:796-799

Heart rate variability is one of major physiological parameters to reflect our stress, which has motivated researchers to investigate a Doppler sensor-based non-contact heartbeat interval estimation algorithm. As one of such methods, we have previously proposed a spectrogram-based method. In this method, the spectrum that might be due to heartbeats is integrated over a spectrogram, and then heartbeat interval is estimated by detecting peaks over the integrated spectrum. However, when a subject moves, undesired peaks with large amplitude appear, which causes the incorrect peak detection. As one of the technique to eliminate the undesired peaks with large amplitude, there is CA-CFAR (Cell Average-Constant False Alarm Rate). CA-CFAR is the technique to detect a signal, when the amplitude of a signal exceeds a threshold calculated with average amplitude of signals before and after the investigated one. However, depending on the duration of body movements, the influence of body movements might be included within the signals used for the threshold calculation, which might results in the detection failure of undesired peaks. This is because the length of GT (Guard Time) is fixed, where GT is the time to prevent the signal used for the threshold calculation from including the investigated signal components. To solve this problem, we propose a novel CA-CFAR in which the length of GT is set as the latest peak interval and only the signal before the investigated one is used so that the influence of body movements does not affect the threshold calculation. Through the experiments where a subject moves, i.e., typing, we confirmed that our spectrogram-based heart rate variability estimation method with the proposed CA-CFAR outperformed the one with CA-CFAR based on fixed GT by the RMSE (Root Mean Squared Error) between the estimated heartbeat interval and the ground truth value of the one.
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http://dx.doi.org/10.1109/EMBC.2019.8857350DOI Listing
July 2019

Dual colonization of Mucoromycotina and Glomeromycotina fungi in the basal liverwort, Haplomitrium mnioides (Haplomitriopsida).

J Plant Res 2019 Nov 15;132(6):777-788. Epub 2019 Oct 15.

Department of Bioscience and Food Production Science, Interdisciplinary Graduate School of Science and Technology, Shinshu University, 8304 Minami-minowa, Nagano, 399-4598, Japan.

In general, Glomeromycotina was thought to be the earliest fungi forming mycorrhiza-like structure (MLS) in land plant evolution. In contrast, because the earliest divergent lineage of extant land plants, i.e. Haplomitriopsida liverworts, associates only with Mucoromycotina mycobionts, recent studies suggested that those fungi are novel candidates for the earliest mycobionts. Therefore, Mucoromycotina-Haplomitriopsida association currently attracts attention as an ancient mycorrhiza-like association. However, mycobionts were identified in only 7 of 16 Haplomitriopsida species and the mycobionts diversity of this lineage is largely unclarified. To clarify the taxonomic composition of mycobionts in Haplomitriopsida, we observed MLSs in the rhizome of Haplomitrium mnioides (Haplomitriopsida), the Asian representative Haplomitriopsida species, and conducted molecular identification of mycobionts. It was recorded for the first time that Glomeromycotina and Mucoromycotina co-occur in Haplomitriopsida as mycobionts. Significantly, the arbuscule-like branching (ALB) of Glomeromycotina was newly described. As the Mucoromycotina fungi forming MLSs in H. mnioides, Endogonaceae and Densosporaceae were detected, in which size differences of hyphal swelling (HS) were found between the fungal families. This study provides a novel evidence in the MLS of Haplomitriopsida, i.e. the existence of Glomeromycotina association as well as the dominant Mucoromycotina association. In addition, since hyphal characteristics of the HS-type MLS were quite similar to those of fine endophytes (FE) of Endogonales in other bryophytes and vascular plants previously described, this MLS is suggested to be included in FE. These results suggest that Glomeromycotina and Mucoromycotina were acquired concurrently as the mycobionts by the earliest land plants evolved into arbuscular mycorrhizae and FE. Therefore, dual association of Haplomitriopsida, with Endogonales and Glomeromycotina will provide us novel insight on how the earliest land plants adapted to terrestrial habitats with fungi.
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http://dx.doi.org/10.1007/s10265-019-01145-3DOI Listing
November 2019

Kinematics of Maximal Speed Sprinting With Different Running Speed, Leg Length, and Step Characteristics.

Front Sports Act Living 2019 26;1:37. Epub 2019 Sep 26.

Faculty of Health and Sports Sciences, University of Tsukuba, Ibaraki, Japan.

This study aimed to provide multiple regression equations taking into account differences in running speed, leg length, and step characteristics to predict kinematics of maximal speed sprinting. Seventy-nine male sprinters performed a maximal effort 60-m sprint, during which they were videoed through the section from the 40- to 50-m mark. From the video images, leg kinematic variables were obtained and used as dependent variables for multiple linear regression equation with predictors of running speed, leg length, step frequency, and swing/support ratio. Multiple regression equations to predict leg kinematics of maximal speed sprinting were successfully obtained. For swing leg kinematics, a significant regression model was obtained to predict thigh angle at the contralateral foot strike, maximal knee flexion and thigh lift angular velocities, and maximal leg backward swing velocity (adjusted = 0.194-0.378, medium to large effect). For support leg kinematics, a significant regression model was obtained to predict knee flexion and extension angular displacements, maximal knee extension velocity, maximal leg backward swing angular velocity, and the other 13 kinematic variables (adjusted = 0.134-0.757, medium to large effect). Based on the results, at a given leg length, faster maximal speed sprinting will be accompanied with greater thigh angle at the contralateral foot strike, greater maximal leg backward swing velocity during the swing phase, and smaller knee extension range during the support phase. Longer-legged sprinters will accomplish the same running speed with a greater thigh angle at contralateral foot strike, greater knee flexion range, and smaller maximal leg backward swing velocity during the support phase. At a given running speed and leg length, higher step frequencies will be achieved with a greater thigh angle at contralateral foot strike and smaller knee flexion and extension ranges during the support phase. At a given running speed, leg length and step frequency, a greater swing/support ratio will be accompanied with a greater thigh angle at contralateral foot strike and smaller knee extension angular displacement and velocity during the support phase. The regression equations obtained in this study will be useful for sprinters when trying to improve their maximal speed sprinting motion.
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http://dx.doi.org/10.3389/fspor.2019.00037DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7739839PMC
September 2019

A randomized controlled, open-label early phase II trial comparing incidence of FOLFIRI.3-induced diarrhoea between Hangeshashinto and oral alkalization in Japanese patients with colorectal cancer.

J Clin Pharm Ther 2019 Dec 13;44(6):946-951. Epub 2019 Aug 13.

Division of Pharmacy, University Hospital, Chiba University School of Medicine, Chiba, Japan.

What Is Known And Objective: We conducted a pilot clinical trial to investigate whether Hangeshashinto (TJ-14) could be substituted for oral alkalization in patients scheduled to undergo chemotherapy by FOLFIRI.3 regimen for colorectal cancer (CRC).

Methods: Patients with CRC were randomized 1:1 to a TJ-14 (7.5 g/day) group or an oral alkalization (sodium bicarbonate, 1.8 g/day; ursodeoxycholic acid, 300 mg/day) group. The primary endpoint was incident of late-onset diarrhoea. A total of 30 patients were randomized to either the TJ-14 group or the alkalization group.

Results And Discussion: There was no statistical difference in age, concomitantly used drugs or UGT1A1 genotypes between the groups. In the alkalization group (n = 15), the frequency of grade 0/1/2 and grade 3 diarrhoea was 73% and 27%, respectively. In the TJ-14 group (n = 14), the frequency of grade 0/1/2 and grade 3 diarrhoea was 79% and 21%, respectively. Grade 4 diarrhoea was not observed in either group. There was no statistically significant difference in other adverse events or in response to FOLFIRI.3 between the groups.

What Is New And Conclusion: This pilot trial suggests that TJ-14 is a promising alternative treatment option to reduce FOLFIRI.3-induced late-onset diarrhoea, although additional clinical study with a larger number of patients is necessary to confirm these results.
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http://dx.doi.org/10.1111/jcpt.13020DOI Listing
December 2019

Fern gametophytes of Angiopteris lygodiifolia and Osmunda japonica harbor diverse Mucoromycotina fungi.

J Plant Res 2019 Sep 10;132(5):581-588. Epub 2019 Jul 10.

Department of Chemical and Biological Sciences, Japan Women's University, 2-8-1 Mejirodai, Bunkyo-ku, Tokyo, 112-8681, Japan.

Mycorrhizal symbiosis between plants and fungi is ubiquitous, and has been played key roles in plant terrestrialization and diversification. Although arbuscular mycorrhizal (AM) symbioses with Glomeromycotina fungi have long been recognized as both ancient and widespread symbionts, recent studies showed that Mucoromycotina fungi were also ancestral symbionts and would thus be expected to co-exist with many land plants. To explore whether Mucoromycotina colonize fern gametophytes, we subjected fungal associations with gametophytes of two distantly related ferns, Angiopteris lygodiifolia (Marattiales) and Osmunda japonica (Osmundales), to molecular analysis. Direct PCR amplification from intracellular hyphal coils was also performed. We detected Mucoromycotina sequences in the gametophytes of A. lygodiifolia and O. japonica at rates of 41% (7/17) and 50% (49/98) of gametophytes, respectively, and assigned them to 10 operational taxonomic units of Endogonales lineages. In addition, we used AM fungal-specific primers and detected Glomeromycotina sequences in all individuals examined. The results suggest that Glomeromycotina and Mucoromycotina colonized fern gametophytes simultaneously. We found that Mucoromycotina were present in fern gametophytes of Marratiales and Osmundales, which implies that a variety of fern taxa have Mucoromycotina associations.
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http://dx.doi.org/10.1007/s10265-019-01121-xDOI Listing
September 2019

Pharmacokinetic interference of doxorubicin with tolbutamide due to reduced metabolic clearance with increased serum unbound fraction in rats.

Biopharm Drug Dispos 2019 Jul 16;40(7):225-233. Epub 2019 Jul 16.

Laboratory of Clinical Pharmacy and Therapeutics, Faculty of Pharmacy, Osaka Ohtani University, 3-11-1 Nishikiori-kita, Tondabayashi, 584-0066, Japan.

The study examined the effect of doxorubicin (DOX) on the hepatic expression of CYP2C and its activity for metabolizing tolbutamide (TB), a specific CYP2C substrate, in rats and whether the pharmacokinetics of tolbutamide were altered by doxorubicin exposure. The expression level of hepatic CYP2C11 was depressed 1 day after doxorubicin administration (day 1), and this effect on CYP2C11 was augmented on day 4. However, the expression level of hepatic CYP2C6 remained unchanged. The activity of tolbutamide 4-hydroxylation in hepatic microsomes was decreased with time following doxorubicin administration. Regarding the enzyme kinetic parameters for tolbutamide 4-hydroxylation on day 4, the maximum velocity (V ) was significantly lower in the DOX group than that in the control group, while the Michaelis constant (K ) was unaffected. On pharmacokinetic examination, the total clearance (CL ) of tolbutamide on day 4 was increased, despite the decreased metabolic capacity. On the other hand, the serum unbound fraction (f ) of tolbutamide was elevated with a reduced serum albumin concentration in the DOX group. Contrary to CL , CL /f , a parameter approximated to the hepatic intrinsic clearance of unbound tolbutamide, was estimated to be significantly reduced in the DOX group. These findings indicate that the metabolic capacity of CYP2C11 in the liver is depressed time-dependently by down-regulation after doxorubicin exposure in rats, and that the decreased enzyme activity of TB 4-hydroxylation in hepatic microsomes reflects the pharmacokinetic change of unbound tolbutamide, not total tolbutamide, in serum.
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http://dx.doi.org/10.1002/bdd.2195DOI Listing
July 2019

The conservation of polyol transporter proteins and their involvement in lichenized Ascomycota.

Fungal Biol 2019 04 31;123(4):318-329. Epub 2019 Jan 31.

Division of Environmental Horticulture, Graduate School of Horticulture, Chiba University, 648 Matsudo, Matsudo, Chiba, 271-0092, Japan. Electronic address:

In lichen symbiosis, polyol transfer from green algae is important for acquiring the fungal carbon source. However, the existence of polyol transporter genes and their correlation with lichenization remain unclear. Here, we report candidate polyol transporter genes selected from the genome of the lichen-forming fungus (LFF) Ramalina conduplicans. A phylogenetic analysis using characterized polyol and monosaccharide transporter proteins and hypothetical polyol transporter proteins of R. conduplicans and various ascomycetous fungi suggested that the characterized yeast' polyol transporters form multiple clades with the polyol transporter-like proteins selected from the diverse ascomycetous taxa. Thus, polyol transporter genes are widely conserved among Ascomycota, regardless of lichen-forming status. In addition, the phylogenetic clusters suggested that LFFs belonging to Lecanoromycetes have duplicated proteins in each cluster. Consequently, the number of sequences similar to characterized yeast' polyol transporters were evaluated using the genomes of 472 species or strains of Ascomycota. Among these, LFFs belonging to Lecanoromycetes had greater numbers of deduced polyol transporter proteins. Thus, various polyol transporters are conserved in Ascomycota and polyol transporter genes appear to have expanded during the evolution of Lecanoromycetes.
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http://dx.doi.org/10.1016/j.funbio.2019.01.006DOI Listing
April 2019

Pharmacokinetics of cisplatin in an esophageal cancer patient on hemodialysis who was treated with a full-dose cisplatin-fluorouracil regimen: A case report.

J Oncol Pharm Pract 2019 Oct 10;25(7):1767-1775. Epub 2018 Oct 10.

1 Department of Frontier Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan.

Introduction: Cancer patients undergoing hemodialysis might be under-treated because the pharmacokinetics of anti-cancer drugs in such patients remain unknown and out of concern related to the potential development of severe adverse effects. However, patients with chemosensitive cancer, such as esophageal cancer, should receive chemotherapy at a dose that is sufficient to attain a favorable therapeutic effect. We herein present an interesting case involving an esophageal cancer patient who was successfully treated with subtotal thoracic esophagectomy, and adjuvant full-dose chemotherapy with cisplatin and 5-fluorouracil while concomitantly undergoing hemodialysis. We carried out a pharmacokinetics analysis of cisplatin, and also conducted a systematic review on the dose and pharmacokinetics.

Case Report: A 57-year-old male patient with esophageal cancer who was undergoing hemodialysis was referred to our hospital. He underwent subtotal thoracic esophagectomy. The pathological diagnosis was T1b, N2 (5/26), M0, ly2, v2, stage IIIA (Union for International Cancer Control, 8th edition). Because of the high degree of lymph node metastasis, adjuvant chemotherapy with cisplatin was recommended. Cisplatin (80 mg/m) was infused intravenously within 30 min on day 1, and 5-fluorouracil (800 mg/m) was infused continuously on days 1-5 of a 28-day cycle. Thrombocytopenia (grade 3) occurred on day 16, leucopenia (grade 3) occurred on day 23, and anemia (grade 3) occurred on day 30. The onset of hematologic toxicities was prolonged in comparison to patients with a normal renal function.
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http://dx.doi.org/10.1177/1078155218808074DOI Listing
October 2019

Pharmacokinetics and metabolic elimination of tolbutamide in female rats: Comparison with male rats.

Biopharm Drug Dispos 2018 Jul 31;39(7):321-327. Epub 2018 Jul 31.

Laboratory of Clinical Pharmacy and Therapeutics, Faculty of Pharmacy, Osaka Ohtani University, 3-11-1 Nishikiori-kita, Tondabayashi, 584-8540, Japan.

As there are to be known gender differences in the expression profiles of rat hepatic CYP2C, we examined the pharmacokinetic behavior of tolbutamide (TB), a typical probe for CYP2C, and hepatic enzyme activities for metabolizing TB in female rats to compare with male rats. On the pharmacokinetic analysis of TB after intravenous administration to female rats, the elimination rate constant at the terminal phase (k ), total clearance (CL ) and the apparent volume of distribution at steady-state (Vd ) were significantly lower than in male rats. The binding rates of TB to serum protein were similar in male and female rats, indicating that the change in unbound TB concentration in serum is not associated with the difference in the pharmacokinetic disposition of TB. On metabolic examination using hepatic microsomes, the maximum reaction velocity (V ) of the metabolic conversion from TB to 4-hydroxytolbutamide (4-OH-TB) in female rats was lower than that in male rats, although there was no significant difference in the Michaelis constant (K ) between genders. Consistent with this, the V -to-K ratio (V /K ) was significantly lower in female rats than in male rats. Therefore, the low in vitro CYP2C-dependent activity for hepatic TB removal in female rats provided a clear explanation for the lower in vivo elimination clearance of TB. Our findings strongly suggest that there is a gender difference in the metabolic capacity to eliminate drugs that serve as substrates of hepatic CYP2C enzymes in rats.
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http://dx.doi.org/10.1002/bdd.2148DOI Listing
July 2018

The data of change in macrophage gene expression which induced by perilipin 1 overexpression.

Data Brief 2018 Aug 15;19:179-182. Epub 2018 May 15.

Department of Rheumatology, Endocrinology and Nephrology, Faculty of Medicine, Graduate School of Medicine, Hokkaido University, Kita 15 Nishi 7, Kita-ku, Sapporo, Hokkaido 060-8638, Japan.

The data presented here are related to the research article entitled "Overexpression of Perilipin1 protects against atheroma progression in apolipoprotein E knockout mice" [1]. This paper describes data that were obtained from perilipin 1 (PLIN1) transgenic mice () regarding atherosclerosis. The main aim of collecting the data was to clarify the role of PLIN1 in the pathophysiology of atherosclerosis. The data were collected from C57BL/6J mice, apolipoprotein E knockout mice () and . The atherosclerotic lesion areas of aorta were 3.3 ± 1.2% in C57BL/6J mice, 14.2 ± 3.2% in , and 5.6 ± 1.9% in . Body weight, gonadal adipose mass and plasma triglyceride concentrations were comparable among the three groups [1]. Furthermore, PLIN1 overexpression did not affect the gene expressions related to cholesterol influx and efflux in macrophage.
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http://dx.doi.org/10.1016/j.dib.2018.05.027DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5993010PMC
August 2018

Identifying Molecular Orientation in a Bulk Heterojunction Film by Infrared Reflection Absorption Spectroscopy.

ACS Omega 2018 May 25;3(5):5678-5684. Epub 2018 May 25.

Graduate School of Natural Science and Technology, Research Center for Sustainable Energy and Technology, and Institute for Frontier Science Initiative (InFiniti), Kanazawa University, Kakuma-machi, 920-1192 Kanazawa, Japan.

The molecular orientation of organic molecules of zinc phthalocyanine (ZnPc) in single-component films on copper iodide (CuI) substrates can be controlled to achieve a molecular orientation lying flat on the substrate (flat-on) owing to π-d orbital interactions between the ZnPc molecules and the CuI. A 3-fold enhancement in the performance of organic photovoltaic cells has been reported by introducing a CuI interlayer between a ZnPc:fullerene (C60) bulk heterojunction (BHJ) film and the substrate. However, the mechanism underpinning the resultant solar cell performance enhancement was unclear. Herein, we report on the results of using in situ reflection absorption spectroscopy measurements during the vacuum deposition of coevaporated ZnPc:C60 BHJ films on various substrates to investigate the ZnPc molecular orientation. Our results revealed that the flat-on molecular orientation of ZnPc molecules in ZnPc:C60 BHJ films on CuI interlayers and flat-on ZnPc substrates can be successfully identified via the strong π-π interactions between the BHJ film and the substrate. The π-π interactions between individual ZnPc molecules are stronger than the π-d interactions between ZnPc molecules and CuI in coevaporated ZnPc:C60 films, as is evident from the molecular orientation of ZnPc, as determined by in situ reflection absorption spectroscopy. Our findings demonstrate that precisely controlling the molecular orientations of the films could enhance organic photovoltaic (OPV) performance. The present work provides important insights that will enable the design of higher performance OPV cells.
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http://dx.doi.org/10.1021/acsomega.8b00099DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6641761PMC
May 2018

Effect of the sodium-glucose cotransporter 2 inhibitor luseogliflozin on pancreatic beta cell mass in db/db mice of different ages.

Sci Rep 2018 05 1;8(1):6864. Epub 2018 May 1.

Department of Rheumatology, Endocrinology and Nephrology, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, Sapporo, Japan.

To examine the effects of luseogliflozin, a sodium-glucose cotransporter 2 inhibitor, on pancreatic beta cell mass in db/db mice of different ages. db/db mice aged 6, 10, 14 and 24 weeks old were fed either standard chow (control group) or standard chow containing 0.01% luseogliflozin (luseo group). After 4 weeks, immunohistochemistry and gene expression tests were conducted. In 6-week-old db/db mice, immunohistochemistry revealed a significant increase in beta cell mass in the luseo group compared with the control group after 4 weeks of treatment. Gene expression profiling of isolated islets showed upregulation Mafa, Pdx1, Ki67 and Ccnd2 in the luseo group. Beta cell mass decreased with age in db/db mice in the control group. Beta cell mass in the luseo group significantly increased compared with the control group regardless of age, although beta cell mass in the 28-week-old luseo group (4 weeks of treatment in 24-week-old db/db mice) was significantly lower than in the 10-week-old luseo group (4 weeks of treatment in 6-week-old db/db mice). Luseogliflozin preserved beta cell mass in db/db mice. The protective effect was more evident in the earlier phase of diabetes.
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http://dx.doi.org/10.1038/s41598-018-25126-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5931598PMC
May 2018

The role of glucokinase and insulin receptor substrate-2 in the proliferation of pancreatic beta cells induced by short-term high-fat diet feeding in mice.

Metabolism 2018 08 12;85:48-58. Epub 2018 Mar 12.

Department of Rheumatology, Endocrinology and Nephrology, Faculty of Medicine and Graduate School of Medicine, Hokkaido University Graduate School of Medicine, Sapporo, Japan.

Objective: We investigated whether glucokinase and insulin receptor substrate-2 were required for beta cell proliferation induced by short-term high-fat (HF) diet feeding, as has been shown for long-term HF diet.

Methods: Eight-week-old C57BL/6J mice were exposed to either a standard chow (SC) or HF diet. After 1 week on the diet, histopathological beta cell proliferation and gene expression in isolated islets were examined. Additionally, 8-week-old beta cell-specific glucokinase haploinsufficient (Gck) and Irs2 knockout (Irs2) mice were exposed to either an SC or HF diet.

Results: Immunohistochemical analysis revealed that short-term HF diet feeding resulted in a significant increase in BrdU incorporation rate compared with SC consumption in wild-type mice. Western blot analysis demonstrated that Irs2 expression levels did not differ between the two diets. Moreover, there was a significant increase in the BrdU incorporation rate in the HF diet group compared with the SC group in both Gck and Irs2 mice. Gene expression profiling of isolated islets from mice fed an HF diet for 1 week revealed that the expression levels of downstream genes of Foxm1 were coordinately upregulated. One week of HF diet feeding stimulated beta cell proliferation with Foxm1 upregulation in 48-week-old mice as well as in 8-week-old.

Conclusions: The mechanism of pancreatic beta cell proliferation induced by short-term HF diet feeding in mice could involve a glucokinase- and Irs2-independent pathway. Our results suggest that the pathways that induce beta cell proliferation in response to short-term HF diet feeding may differ from those in response to sustained HF diet feeding.
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http://dx.doi.org/10.1016/j.metabol.2018.03.010DOI Listing
August 2018

Overexpression of perilipin1 protects against atheroma progression in apolipoprotein E knockout mice.

Atherosclerosis 2018 02 17;269:192-196. Epub 2018 Jan 17.

Hokkaido University, Kita-ku, Sapporo 060-8638, Japan.

Background And Aims: Perilipin1 (PLIN1), a lipid droplet-associated protein, plays an important role in the regulation of lipolysis and lipid storage in adipocytes. PLIN1 has recently been reported to be expressed in macrophages within atheroma plaques, suggesting PLIN1 may play a role in the accumulation of lipids at the arterial wall and in the development of atherosclerosis. To clarify the role of PLIN1 in the pathophysiology of atherosclerosis, we assessed the progression of atherosclerosis in PLIN1 transgenic mice (Plin1Tg).

Methods: Plin1Tg were crossed with apolipoprotein E knockout mice (ApoeKO). C57BL/6J mice, ApoeKO and Plin1Tg/ApoeKO received a normal chow diet for 20 weeks. Body weight, gonadal fat mass and plasma lipid concentrations were measured. Aortas were collected for quantification of atheroma lesions and histological analysis by Oil Red O staining.

Results: Body weight, gonadal adipose mass and plasma triglyceride concentrations were not significantly different among the three groups. In contrast, the atherosclerotic lesion area was significantly increased in ApoeKO (14.2 ± 3.2%; p < .01) compared with C57BL/6J mice (3.3 ± 1.2%) and Plin1Tg/ApoeKO (5.6 ± 1.9%).

Conclusions: Overexpressed PLIN1 in macrophages had a protected role against atheroma progression in ApoeKO in the absence of changes in gonadal fat mass or plasma lipid levels, presumably due to modification of the stability and/or inflammatory profile of macrophages.
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http://dx.doi.org/10.1016/j.atherosclerosis.2018.01.019DOI Listing
February 2018

Satisfaction and efficacy of switching from daily dipeptidyl peptidase-4 inhibitors to weekly trelagliptin in patients with type 2 diabetes-Randomized controlled study.

Endocr J 2018 Feb 31;65(2):141-150. Epub 2017 Oct 31.

Division of Rheumatology, Endocrinology and Nephrology, Faculty of Medicine and Graduate School of Medicine, Hokkaido University Hospital, North 15, West 7, Kita-ku, Sapporo 060-8638, Japan.

We compared treatment satisfaction between daily dipeptidyl peptidase-4 (DPP-4) inhibitors and a weekly DPP-4 inhibitor in patients with type 2 diabetes. The study was a 12-week, open-label, randomized, multicenter, controlled trial. Participants were Japanese patients with type 2 diabetes who had received daily DPP-4 inhibitors for more than 3 months. Patients were randomly assigned to a treatment cohort: (1) a group that continued taking daily DPP-4 inhibitors (daily group); or (2) a group that switched from daily DPP-4 inhibitors to a weekly DPP-4 inhibitor, trelagliptin (weekly group). The primary outcome was the change in treatment satisfaction levels from baseline to 12 weeks between the two groups, according to Diabetes Treatment Satisfaction Questionnaire (DTSQ) and Diabetes Therapy-Related Quality of Life (DTR-QOL) questionnaire scores. The changes in glycemic control and body weight were also assessed. Of 49 patients initially enrolled in the study, 47 completed the study. The change in DTSQ scores in the weekly group was not significantly different from that in the daily group. However, the improvements in total score and subscale domains 1 and 2 in the DTR-QOL analysis, which relate to burden on social/daily activities and anxiety/dissatisfaction with treatment, were significantly greater in the weekly group than the daily group (p = 0.048, 0.013 and 0.045, respectively). Mean changes in glycated hemoglobin levels and body weight were comparable between the groups. Switching from daily DPP-4 inhibitors to a weekly DPP-4 inhibitor, trelagliptin, could partially improve treatment satisfaction levels in patients with type 2 diabetes without affecting glycemic control.
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http://dx.doi.org/10.1507/endocrj.EJ17-0303DOI Listing
February 2018

Improved Reproducibility and Intercalation Control of Efficient Planar Inorganic Perovskite Solar Cells by Simple Alternate Vacuum Deposition of PbI and CsI.

ACS Omega 2017 Aug 11;2(8):4464-4469. Epub 2017 Aug 11.

Institute for Frontier Science Initiative (InFiniti), Graduate School of Natural Science and Technology, and Research Center for Sustainable Energy and Technology, Kanazawa University, Kakuma-machi, 920-1192 Kanazawa, Japan.

Vacuum deposition is a simple and controllable approach that aims to form higher-quality perovskite films compared with those formed using solution-based deposition processes. Herein, we demonstrate a novel method to promote the intercalation control of inorganic cesium lead iodide (CsPbI) perovskite thin films via alternate vacuum deposition. We also investigated the effect of layer-by-layer deposition of PbI/CsI to fabricate efficient planar heterojunction CsPbI thin films and solar cells. This procedure is comparatively simple when compared with commonly used coevaporation techniques; further, precise intercalation control of the CsPbI thin films can be achieved by increasing the number of layers in the layer-by-layer deposition of PbI/CsI. The best control and the highest reproducibility were achieved for the deposition of four double layers owing to the precise intercalation control during the deposition of the CsPbI thin film. A power conversion efficiency of 6.79% was obtained via alternating vacuum deposition of two double layers with a short-circuit current density ( ) of 12.06 mA/cm, an open-circuit voltage ( ) of 0.79 V, and a fill factor (FF) of 0.72. Our results suggest a route for inorganic precursors to be used for efficient perovskite solar cells via alternating vacuum deposition.
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http://dx.doi.org/10.1021/acsomega.7b00814DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6641724PMC
August 2017
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