Publications by authors named "Kiattisak Promsuwan"

6 Publications

  • Page 1 of 1

Discrimination of dopamine by an electrode modified with negatively charged manganese dioxide nanoparticles decorated on a poly(3,4 ethylenedioxythiophene)/reduced graphene oxide composite.

J Colloid Interface Sci 2021 Mar 31;597:314-324. Epub 2021 Mar 31.

Center of Excellence for Trace Analysis and Biosensor, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand; Center of Excellence for Innovation in Chemistry, Faculty of Science, Prince of Songkla University, HatYai, Songkhla 90112, Thailand; Division of Health and Applied Sciences, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand. Electronic address:

A unique nanocomposite was fabricated using negatively charged manganese dioxide nanoparticles, poly (3,4-ethylenedioxythiophene) and reduced graphene oxide (MnO/PEDOT/rGO). The nanocomposite was deposited on a glassy carbon electrode (GCE) functionalized with amino groups. The modified GCE was used to electrochemically detect dopamine (DA). The surface morphology, charge effect and electrochemical behaviours of the modified GCE were characterized by scanning electron microscopy, energy dispersive X-ray analysis (EDX), cyclic voltammetry and electrochemical impedance spectroscopy, respectively. The MnO/PEDOT/rGO/GCE exhibited excellent performance towards DA sensing with a linear range between 0.05 and 135 µM with a lowest detection limit of 30 nM (S/N = 3). Selectivity towards DA was high in the presence of high concentrations of the typical interferences ascorbic acid and uric acid. The stability and reproducibility of the electrode were good. The sensor accurately determined DA in human serum. The synergic effect of the multiple components of the fabricated nanocomposite were critical to the good DA sensing performance. rGO provided a conductive backbone, PEDOT directed the uniform growth of MnO and adsorbed DA via pi-pi and electrostatic interaction, while the negatively charged MnO provided adsorption and catalytic sites for protonated DA. This work produced a promising biosensor that sensitively and selectively detected DA.
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http://dx.doi.org/10.1016/j.jcis.2021.03.162DOI Listing
March 2021

A portable electrochemical sensor for detection of the veterinary drug xylazine in beverage samples.

J Pharm Biomed Anal 2021 May 8;198:113958. Epub 2021 Feb 8.

Center of Excellence for Trace Analysis and Biosensors (TAB-CoE), Prince of Songkla University, Hat Yai, Songkhla, 90112, Thailand; Center of Excellence for Innovation in Chemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, 90112, Thailand; Division of Health and Applied Sciences, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, 90112, Thailand. Electronic address:

A portable electrochemical sensor was developed to determine xylazine in spiked beverages by adsorptive stripping voltammetry (AdSV). The sensor was based on a graphene nanoplatelets-modified screen-printed carbon electrode (GNPs/SPCE). The electrochemical behavior of xylazine at the GNPs/SPCE was an adsorption-controlled irreversible oxidation reaction. The loading of graphene nanoplatelets (GNPs) on the modified SPCE, electrolyte pH, and AdSV accumulation potential and time were optimized. Under optimal conditions, the GNPs/SPCE provided high sensitivity, linear ranges of 0.4-6.0 mg L (r = 0.997) and 6.0-80.0 mg L (r = 0.998) with a detection limit of 0.1 mg L and a quantitation limit of 0.4 mg L. Repeatability was good. The accuracy of the proposed sensor was investigated by spiking six beverage samples at 1.0, 5.0, and 10.0 mg L. The recoveries from this method ranged from 80.8 ± 0.2-108.1 ± 0.3 %, indicating the good accuracy of the developed sensor. This portable electrochemical sensor can be used to screen for xylazine in beverage samples as evidence in cases of sexual assault or robbery.
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http://dx.doi.org/10.1016/j.jpba.2021.113958DOI Listing
May 2021

Subnanomolar detection of promethazine abuse using a gold nanoparticle-graphene nanoplatelet-modified electrode.

Mikrochim Acta 2020 Nov 9;187(12):646. Epub 2020 Nov 9.

Center of Excellence for Trace Analysis and Biosensor, Prince of Songkla University, Hat Yai, Songkhla, 90112, Thailand.

A simple, sensitive, and effective adsorptive stripping voltammetric sensor for the detection of trace-level promethazine was created based on a gold nanoparticle-graphene nanoplatelet-modified glassy carbon electrode (AuNP-GrNP/GCE). AuNP-GrNP nanocomposites were synthesized using an electroless deposition process, and the morphology was characterized using UV-vis spectroscopy, Fourier transform infrared spectroscopy, field emission scanning electron microscopy, and energy-dispersive X-ray spectroscopy. The electrochemical behavior and detection of promethazine at the AuNP-GrNP/GCE were investigated utilizing cyclic voltammetry and adsorptive stripping voltammetry. The AuNP-GrNP/GCE showed outstanding synergistic electrochemical activity for promethazine oxidation, a highly active surface area, great adsorptivity, and outstanding catalytic properties. The electrolyte pH, amount of AuNP-GrNP nanocomposite, preconcentration potential (vs. Ag/AgCl), and time were optimized to obtain a high performance electrochemical sensor. Under optimal conditions, the proposed sensor displayed two linear concentration ranges from 1.0 nmol L to 1.0 μmol L and from 1.0 to 10 μmol L. The limits of detection and quantitation were 0.40 and 1.4 nmol L, respectively. This sensor displayed high sensitivity, a capability for rapid analysis, and excellent repeatability and reproducibility. The developed sensor was effective and practical for promethazine detection in biological fluids and forensic samples, and the obtained results exhibited excellent agreement with the results obtained using the method described in the British Pharmacopoeia. Graphical abstract.
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http://dx.doi.org/10.1007/s00604-020-04616-wDOI Listing
November 2020

Porous palladium-poly(3,4-ethylenedioxythiophene)-coated carbon microspheres/graphene nanoplatelet-modified electrode for flow-based-amperometric hydrazine sensor.

Mikrochim Acta 2020 09 2;187(9):539. Epub 2020 Sep 2.

Center of Excellence for Trace Analysis and Biosensor, Prince of Songkla University, Hat Yai, Songkhla, 90112, Thailand.

A highly stable flow-injection amperometric hydrazine sensor was developed based on a glassy carbon electrode modified with palladium-poly(3,4-ethylene dioxythiophene) coated on carbon microspheres/graphene nanoplatelets (Pd-PEDOT@CM/GNP/GCE). The Pd-PEDOT@CM/GNP composite was characterized by scanning electron microscopy and energy-dispersive x-ray analysis (SEM/EDX). The modified GCE was electrochemically characterized using cyclic voltammetry and chronoamperometry. The electrocatalytic activity of the Pd-PEDOT@CM/GNP/GCE toward hydrazine oxidation was significantly better than the activity of a bare GCE, a CM/GCE, a GNP/GCE, a Pd-PEDOT/GCE, and a Pd-PEDOT@CM/GCE. The sensor operated best at a low working potential of + 0.10 V (vs. Ag/AgCl). Under optimal conditions, sensitivity toward hydrazine detection and operational stability (601 injections/one electrode preparation) were excellent. The response was linear from 1.0 to 100 μmol L and from 100 to 5000 μmol L with a detection limit of 0.28 ± 0.02 μmol L and high sensitivity of 0.200 μA μM cm. The sensor showed good repeatability (relative standard deviation (RSD) < 1.4%, n = 15), reproducibility (RSD < 2.7%, n = 6), and anti-interference characteristics toward hydrazine detection. The feasibility of the electrochemical sensor was proved by the successful determination of hydrazine in water samples, and the results were in good agreement with those obtained from spectrophotometric analysis. Graphical abstract.
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http://dx.doi.org/10.1007/s00604-020-04470-wDOI Listing
September 2020

Bio-PEDOT: Modulating Carboxyl Moieties in Poly(3,4-ethylenedioxythiophene) for Enzyme-Coupled Bioelectronic Interfaces.

ACS Appl Mater Interfaces 2020 Sep 20;12(35):39841-39849. Epub 2020 Aug 20.

Biosensors and Bioelectronics Centre, Department of Physics, Chemistry and Biology, Linköping University, SE-581 83 Linköping, Sweden.

Modulation of chemical functional groups on conducting polymers (CPs) provides an effective way to tailor the physicochemical properties and electrochemical performance of CPs, as well as serves as a functional interface for stable integration of CPs with biomolecules for organic bioelectronics (OBEs). Herein, we introduced a facile approach to modulate the carboxylate functional groups on the PEDOT interface through a systematic evaluation on the effect of a series of carboxylate-containing molecules as counterion dopant integrated into the PEDOT backbone, including acetate as monocarboxylate (mono-COO), malate as dicarboxylate (di-COO), citrate as tricarboxylate (tri-COO), and poly(acrylamide--acrylate) as polycarboxylate (poly-COO) bearing different amounts of molecular carboxylate moieties to create tunable PEDOT:COO interfaces with improved polymerization efficiency. We demonstrated the modulation of PEDOT:COO interfaces with various granulated morphologies from 0.33 to 0.11 μm, tunable surface carboxylate densities from 0.56 to 3.6 μM cm, and with improved electrochemical kinetics and cycling stability. We further demonstrated the effective and stable coupling of an enzyme model lactate dehydrogenase (LDH) with the optimized PEDOT:poly-COO interface via simple covalent chemistry to develop biofunctionalized PEDOT (Bio-PEDOT) as a lactate biosensor. The biosensing mechanism is driven by a sequential bioelectrochemical signal transduction between the bio-organic LDH and organic PEDOT toward the concept of all-polymer-based OBEs with a high sensitivity of 8.38 μA mM cm and good reproducibility. Moreover, we utilized the LDH-PEDOT biosensor for the detection of lactate in spiked serum samples with a high recovery value of 91-96% and relatively small RSD in the range of 2.1-3.1%. Our findings provide a new insight into the design and optimization of functional CPs, leading to the development of new OBEs for sensing, biosensing, bioengineering, and biofuel cell applications.
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http://dx.doi.org/10.1021/acsami.0c10270DOI Listing
September 2020

Adsorption and determination of sibutramine in illegal slimming product using porous graphene ink-modified electrode.

Talanta 2020 May 27;212:120788. Epub 2020 Jan 27.

Department of Applied Science, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, 90112, Thailand; Center of Excellence for Trace Analysis and Biosensor, Prince of Songkla University, Hat Yai, Songkhla, 90112, Thailand; Center of Excellence for Innovation in Chemistry, Faculty of Science, Prince of Songkla University, HatYai, Songkhla, 90112, Thailand. Electronic address:

This work presents a new material, i.e., a porous graphene ink to modify on the GCE surface (PGr-ink/GCE) to improve sensitivity by increasing the surface area of the electrode for the electrochemical determination of sibutramine. The surface characterization and electrochemical adsorption behavior of the PGr-ink/GCE toward sibutramine were investigated using scanning electron microscopy (SEM), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), ATR-FTIR spectroscopy and square wave adsorptive stripping voltammetry (SWAdSV). The PGr-ink/GCE exhibited a distinctive anodic response towards sibutramine using SWAdSV in BR buffer (pH 8.00) with a sensitivity 4 times higher than the bare GCE. Under the optimum conditions, the modified electrode showed good electrochemical adsorption and detection of sibutramine by the SWAdSV method with two ranges of linear concentration from 0.015 to 10.0 μg mL and 10-50 μg mL. The limit of detection and quantitation were 5 ng mL and 15 ng mL, respectively. Over a short analysis time (180 s), the sensor exhibited high sensitivity (10.9 μAμgmLcm), good reproducibility (RSD <3.4% n = 6), repeatability (RSD between 1.8 and 9.8% (n = 15)), excellent anti-interference properties and was successfully applied for the quantification of sibutramine in different brands of illegal slimming products with good recoveries (93 ± 4-104 ± 1%). The developed electrochemical sibutramine sensor is suitable for application in detecting illegal components in slimming products.
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http://dx.doi.org/10.1016/j.talanta.2020.120788DOI Listing
May 2020