Publications by authors named "Keqiang Wang"

56 Publications

Does natural resources supervision improve construction land use efficiency: Evidence from China.

J Environ Manage 2021 Jul 26;297:113317. Epub 2021 Jul 26.

School of Finance and Business, Shanghai Normal University, Shanghai, 200234, China.

Based on data from 286 cities, this paper analyzes the realistic effect of natural resource supervision on construction land use efficiency via the difference-in-differences (DID) method and its potential effect via stochastic frontier analysis (SFA). First, in the real economy, natural resource supervision can significantly reduce construction land use efficiency in cities with a natural resource supervision agency (NRSA), but the inhibitory effect gradually weakens. Natural resource supervision can promote the market development of tender, auction and quotation (TAQ) and thereby have an indirect impact by strengthening illegal land management. Second, natural resource supervision can significantly improve construction land use efficiency in cities with an NRSA when the degree of vertical financial asymmetry is high, but it has the opposite effect when the fiscal revenue decentralization level, regional competition level, economic development level and so on are high. Third, in terms of the potential effect, construction land use efficiency in cities without an NRSA is decreasing annually. Natural resource supervision can greatly promote construction land use efficiency in cities with an NRSA and allow these cities to catch up with cities without an NRSA. From the logical starting point of the resident effect of natural resource supervision, this paper focuses on the ultimate goal of high-quality economic development. This study is of great value for reforming the natural resource supervision system, standardizing the development of the land market and perfecting the incentive mechanism for local governments.
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http://dx.doi.org/10.1016/j.jenvman.2021.113317DOI Listing
July 2021

Multi-spectral intravascular photoacoustic/ultrasound/optical coherence tomography tri-modality system with a fully-integrated 0.9-mm full field-of-view catheter for plaque vulnerability imaging.

Biomed Opt Express 2021 Apr 9;12(4):1934-1946. Epub 2021 Mar 9.

Research Center for Biomedical Optics and Molecular Imaging, Shenzhen Key Laboratory for Molecular Imaging, Guangdong Provincial Key Laboratory of Biomedical Optical Imaging Technology, CAS Key Laboratory of Health Informatics, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China.

Myocardial infarctions are most often caused by the so-called vulnerable plaques, usually featured as non-obstructive lesions with a lipid-rich necrotic core, thin-cap fibroatheroma, and large plaque size. The identification and quantification of these characteristics are the keys to evaluate plaque vulnerability. However, single modality intravascular methods, such as intravascular ultrasound, optical coherence tomography and photoacoustic, can hardly achieve all the comprehensive information to satisfy clinical needs. In this paper, for the first time, we developed a novel multi-spectral intravascular tri-modality (MS-IVTM) imaging system, which can perform 360° continuous rotation and pull-backing with a 0.9-mm miniature catheter and achieve simultaneous acquisition of both morphological characteristics and pathological compositions. Intravascular tri-modality imaging demonstrates the ability of our MS-IVTM system to provide macroscopic and microscopic structural information of the vessel wall, with identity and quantification of lipids with multi-wavelength excitation. This study offers clinicians and researchers a novel imaging tool to facilitate the accurate diagnosis of vulnerable atherosclerotic plaques. It also has the potential of clinical translations to help better identify and evaluate high-risk plaques during coronary interventions.
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http://dx.doi.org/10.1364/BOE.420724DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8086469PMC
April 2021

Supervision behaviours of customs supervisors on solid waste import in Shanghai, People's Republic of China.

Waste Manag Res 2021 Apr 27:734242X211001413. Epub 2021 Apr 27.

School of Public Economics and Administration, Shanghai University of Finance and Economics, Shanghai, People's Republic of China.

Waste management is a key challenge the world currently faces. Solid waste imports (SWIs) are counteractive to the construction of ecological civilization. The comprehensive prohibition of "foreign waste" (FW) imports (FWIs) is an iconic measure to promote ecological civilization and ecological environment safety. Strengthening the People's Republic of China (PRC)'s supervision of SWI (SSWIs) is an important means of implementing the comprehensive prohibition of FWIs, while existing research has paid little attention to the behaviour of SSWIs. This paper firstly analyses the influential factors of the behaviour of SSWIs based on the theory of planned behaviour (TPB), and five corresponding research hypotheses are put forward accordingly. Then, it uses 262 micro questionnaires regarding the behaviour of SSWIs in the Port of Waigaoqiao in Shanghai, PRC, and constructs a structural equation model based on the TPB to identify the influential factors and effects of the behaviour of SSWIs. Some interesting findings are observed in this research. Firstly, it indicates that attitude, subjective norms (SNs) and perceived behavioural control (PBC), all indirectly act upon supervision behaviour by influencing supervision intention. Secondly, PBC acts directly on the behaviour of SSWIs, which is consistent with the TPB. Thirdly, the direct effects on supervision intention from strongest to weakest are SNs, attitude, and PBC, respectively. The conclusion provides important policy enlightenment for the supervision optimization of the behaviour of SSWIs. The supervision intention can be motivated from various angles by strengthening the attitude, SNs and PBC of customs supervisors, subsequently strengthening their supervision intention and behaviour.
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http://dx.doi.org/10.1177/0734242X211001413DOI Listing
April 2021

Bioinformatics analysis of Myelin Transcription Factor 1.

Technol Health Care 2021 ;29(S1):441-453

School of Precision Instruments and Opto-Electronics Engineering, Tianjin University, Tianjin, China.

Background And Objective: We aimed to further study the role of Myelin Transcription Factor 1(MyT1) in tumor and other diseases and epigenetic regulation, and better understand the regulatory mechanism of MyT1.

Methods: Using bioinformatics analysis, the structure and function of MyT1sequence were predicted and analyzed using bioinformatics analysis, and providing a theoretical basis for further experimental verification and understanding the regulatory mechanism of MyT1. The first, second and third-level structures of MyT1 were predicted and analyzed by bioinformatics analysis tools.

Results: MyT1 is found to be an unstable hydrophilic protein, rather than a secretory protein, with no signal peptide or trans-membrane domain; total amino acids located on the surface of the cell membrane. It contains seven zinc finger domains structurally. At sub-cellular level, MyT1 is localized in the nucleus. The phosphorylation site mainly exists in serine, and its secondary structure is mainly composed of random coils and alpha helices; the three-dimensional structure is analyzed by modeling.

Conclusions: In this study, the structure and function of MyT1 protein were predicted, thereby providing a basis for subsequent expression analysis and functional research; it laid the foundation for further investigation of the molecular mechanism involved in the development of diseases.
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http://dx.doi.org/10.3233/THC-218042DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8150646PMC
January 2021

Influential factors affecting the generation of kitchen solid waste in Shanghai, China.

J Air Waste Manag Assoc 2021 Apr 10;71(4):501-514. Epub 2021 Feb 10.

School of Finance and Business, Shanghai Normal University, Shanghai, People's Republic of China.

The influential factors of the generation of kitchen solid waste (KSW) are vital to design policies that target effective KSW reductions and achieve sustainable KSW management, but has not been extensively studied. The study aims at the first step of KSW management only, i.e., generation, which is the first KSW analysis survey in Shanghai to comprehensively investigate the characteristics and influential factors of KSW generation (KSWG). The data were collected from August to October 2019, which resulted in a total of 433 usable questionnaires regarding KSWG in Shanghai, China. The average rate of KSWG is 6.038 kg/household/week or 0.265 kg/person/day. The average per-person number of KSWG bags is 0.295. The regression results show that the characteristics of KSWG are related to household size, household income, the habit of ordering takeout, district of residence, and meticulous consumption culture. Some interesting findings are observed in the present study. Firstly, there is a nonlinear relationship between KSWG and household size, which can be explained by "common consumption". Secondly, there is a nonlinear relationship between KSWG and household income, which can be explained by the Engel coefficient, which also provides supporting evidence for the environmental Kuznets curve (EKC) hypothesis. Finally, the habit of ordering takeout, district of residence, and meticulous consumption culture are all influential factors of KSWG. The results of the study can improve public awareness and knowledge of KSWG, and suggestions on the minimization of KSWG are put forward, thus will serve as a new reference for the sustainable KSW management of other cities, especially cities in developing countries.: In the practice of sustainable waste management, KSWG is a complex issue that requires a broad analytic approach that considers several factors simultaneously. From a practical perspective, policy decision-makers should pay attention to at least four important actions to reduce KSWG: Firstly, compared with urban areas, rural areas are important areas for reducing KSWG, which should be given sufficient attention. Secondly, compared with low-income households, high-income households are the focus of KSWG reduction and should be considered. Thirdly, meticulous consumption culture plays an important role in promoting the reduction of KSWG, and this culture should be promoted and cultivated in the formulation of actual waste management policies. This is also an important way to reduce KSWG and should be fully considered. Finally, in the pilot process of domestic waste separation in Chinese cities, 3 R (Reduce, Reuse, and Recycle) waste management concept and method in circular economy can be applied to KSW treatment, and adequate financial support is necessary. Through strengthening the management of KSW, the potential value of KSW can be further developed, so as to realize KSWG reduction and resource utilization.
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http://dx.doi.org/10.1080/10962247.2020.1856215DOI Listing
April 2021

A mathematical model for predicting the changes of non-small cell lung cancer based on tumor mass during radiotherapy.

Phys Med Biol 2019 11 26;64(23):235006. Epub 2019 Nov 26.

Department of Radiation Oncology, Tianjin Medical University General Hospital, No. 154, Anshan Road, Heping District, Tianjin 300052, People's Republic of China.

This study aims to build a feasible mathematical model to analyze the mass evolution of NSCLC during standard fractionated radiotherapy. Seventy-three cases of NSCLC who were received radiotherapy with prescription dose of 2 Gy  ×  30 fx were selected retrospectively and divided into adenocarcinoma (ADC) group and squamous cell carcinoma (SCC) group according to the pathological type. A total of six sets of CT/CBCT images were collected. The tumor masses were measured according to each set of images. We build a mathematical model (Linear Quadratic_Repopulation&Reoxygenation& Dissolution model, LQ_RRD model), which was used to fit the first five sets of measured mass into a smooth curve. By adjusting the model parameters (λ, ν and µ), the optimal fitting results can be obtained. In order to verify the accuracy of model prediction, we measured the mass of the review images (MV, measured values), and found out the estimate point of the corresponding time (EV, estimated value) on the fitting curve. The difference and correlation between MV and EV were compared. It was found that the model could substantially simulate the tumor mass changes during radiotherapy, and it had a good fit to the clinical data (%RMSE-Median  =  5.52, %RMSE-Range  =  [3.19, 10.73]). Comparing the differences of model parameters between ADC and SCC group, there was no significant difference in λ (t  =  1.622, p   =  0.109), but the difference was significant in ν and µ (z  =  -7.270, p   =  0.000 and t  =  -10.205, p   =  0.000). Moreover, linear correlation analysis showed that there was a linear correlation between MV and EV no matter mass or volume (r  =  0.960, p   =  0.000 versus r  =  0.926, p   =  0.000). Nevertheless, the deviation between MV and EV of volume was larger than that of mass (z  =  -1.897, p   =  0.058 versus z  =  -3.387, p   =  0.001), and the deviation was more pronounced in larger tumors. We suggest that this mathematical model is more suitable to predict the tumor mass than volume for NSCLC during radiotherapy.
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http://dx.doi.org/10.1088/1361-6560/ab47c0DOI Listing
November 2019

Characterization of microvessels and parenchyma in in-line phase contrast imaging CT: healthy liver, cirrhosis and hepatocellular carcinoma.

Quant Imaging Med Surg 2019 Jun;9(6):1037-1046

Department of Radiation Oncology, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Ji'nan 250117, China.

Background: Hepatocellular carcinoma (HCC) is a cancer with a poor prognosis, and approximately 80% of HCC cases develop from cirrhosis. Imaging techniques in the clinic seem to be insufficient for revealing the microstructures of liver disease. In recent years, phase contrast imaging CT (PCI-CT) has opened new avenues for biomedical applications owing to its unprecedented spatial and contrast resolution. The aim of this study was to present three-dimensional (3D) visualization of human healthy liver, cirrhosis and HCC using a PCI-CT technique called in-line phase contrast imaging CT (ILPCI-CT) and to quantitatively evaluate the variations of these tissues, focusing on the liver parenchyma and microvasculature.

Methods: Tissue samples from 9 surgical specimens of normal liver (n=3), cirrhotic liver (n=2), and HCC (n=4) were imaged using ILPCI-CT at the Shanghai Synchrotron Radiation Facility (SSRF) without contrast agents. 3D visualization of all liver samples are presented. To quantitatively evaluate the vessel features, the vessel branch angles of each sample were clearly depicted. Additionally, radiomic features of the liver parenchyma extracted from the 3D images were measured. To evaluate the stability of the features, the percent coefficient of variation (%COV) was calculated for each radiomic feature. A %COV <30 was considered to be low variation. Finally, one-way ANOVA, followed by Tukey's test, was used to determine significant changes among the different liver specimens.

Results: ILPCI-CT allows for a clearer view of the architecture of the vessels and reveals more structural details than does conventional radiography. Combined with the 3D visualization technique, ILPCI-CT enables the acquisition of an accurate description of the 3D vessel morphology in liver samples. Qualitative descriptions and quantitative assessment of microvessels demonstrated clear differences among human healthy liver, cirrhotic liver and HCC. In total, 38 (approximately 51%) radiomic features had low variation, including 11 first-order features, 16 GLCM features, 6 GLRLM features and 5 GLSZM features. The differences in the mean vessel branch angles and 3 radiomic features (first-order entropy, GLCM-inverse variance and GLCM-sum entropy) were statistically significant among the three groups of samples.

Conclusions: ILPCI-CT may allow for morphologic descriptions and quantitative evaluation of vessel microstructures and parenchyma in human healthy liver, cirrhotic liver and HCC. Vessel branch angles and radiomic features extracted from liver parenchyma images can be used to distinguish the three kinds of liver tissues.
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http://dx.doi.org/10.21037/qims.2019.06.12DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6629555PMC
June 2019

Potential of serum procalcitonin in predicting bacterial exacerbation and guiding antibiotic administration in severe COPD exacerbations: a systematic review and meta-analysis.

Infect Dis (Lond) 2019 09 29;51(9):639-650. Epub 2019 Jul 29.

a Department of Respiratory and Critical Care Medicine, Peking University People's Hospital , Beijing , China.

The value of procalcitonin (PCT) in the diagnosis of bacterial infections and for determining antibiotic usage among patients with acute exacerbations of chronic obstructive pulmonary disease (AECOPD) is currently unclear. We systematically reviewed the literature and selected studies that evaluated PCT as a biomarker for predicting bacterial infection and compared PCT-based protocols to determine its application in the initiation or discontinuation of antibiotics. Guidance for systematic reviews from Cochrane and the GRADE were followed to perform this study. Data were pooled and analyzed by using a random-effects or a fixed-effects model based on the heterogeneity. The pooled sensitivity and specificity of PCT in diagnosing respiratory bacterial infections were 0.60 and 0.76, respectively, with the area under the summary receiver operating characteristic curve of 0.77. Subgroup analysis showed that the sensitivity and specificity of PCT for patients in ICU were 0.48 and 0.69, respectively. PCT-based protocols decreased antibiotic prescription (relative risk = 0.66, 95% CI: 0.62-0.71) and total antibiotic exposure (mean difference = -2.60, 95% CI: -4.48-0.72), without affecting clinical outcomes such as treatment failure, length of hospitalization and rates of re-exacerbation or overall mortality. PCT has a moderate ability to distinguish bacterial respiratory infection in patients with AECOPD. PCT-guided algorithm can reduce unnecessary administration of antibiotics without increasing adverse outcomes. However, for patients requiring admission in the ICU, PCT may have a poor diagnostic value, and the PCT-guided algorithm may not effectively and safely reduce the antibiotic exposure.
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http://dx.doi.org/10.1080/23744235.2019.1644456DOI Listing
September 2019

A potential field segmentation based method for tumor segmentation on multi-parametric MRI of glioma cancer patients.

BMC Med Imaging 2019 06 17;19(1):48. Epub 2019 Jun 17.

Department of Biomedical Engineering, Tianjin University, 92 Weijin Road, Tianjin, 300072, China.

Background: Accurate segmentation of brain tumors is vital for the gross tumor volume (GTV) definition in radiotherapy. Functional MR images like apparent diffusion constant (ADC) and fractional anisotropy (FA) images can provide more comprehensive information for sensitive detection of the GTV. We synthesize anatomical and functional MRI for accurate and semi-automatic segmentation of GTVs and improvement of clinical efficiency.

Methods: Four MR image sets including T1-weighted contrast-enhanced (T1C), T2-weighted (T2), apparent diffusion constant (ADC) and fractional anisotropy (FA) images of 5 glioma patients were acquired and registered. A new potential field segmentation (PFS) method was proposed based on the concept of potential field in physics. For T1C, T2 and ADC images, global potential field segmentation (global-PFS) was used on user defined region of interest (ROI) for rough segmentation and then morphologically processed for accurate delineation of the GTV. For FA images, white matter (WM) was removed using local potential field segmentation (local-PFS), and then tumor extent was delineated with region growing and morphological methods. The individual segmentations of multi-parametric images were ensembled into a fused segmentation, considered as final GTV. GTVs were compared with manually delineated ground truth and evaluated with segmentation quality measure (Q), Dice's similarity coefficient (DSC) and Sensitivity and Specificity.

Results: Experimental study with the five patients' data and new method showed that, the mean values of Q, DSC, Sensitivity and Specificity were 0.80 (±0.07), 0.88 (±0.04), 0.92 (±0.01) and 0.88 (±0.05) respectively. The global-PFS used on ROIs of T1C, T2 and ADC images can avoid interferences from skull and other non-tumor areas. Similarity to local-PFS on FA images, it can also reduce the time complexity as compared with the global-PFS on whole image sets.

Conclusions: Efficient and semi-automatic segmentation of the GTV can be achieved with the new method. Combination of anatomical and functional MR images has the potential to provide new methods and ideas for target definition in radiotherapy.
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http://dx.doi.org/10.1186/s12880-019-0348-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6580466PMC
June 2019

Erratum to rhinovirus is associated with severe adult community-acquired pneumonia in China.

J Thorac Dis 2018 12;10(12):E861

Department of Respiratory and Critical Care Medicine, Peking University People's Hospital, Beijing 100044, China.

[This corrects the article DOI: 10.21037/jtd.2017.10.107.].
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http://dx.doi.org/10.21037/jtd.2018.11.11DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6344775PMC
December 2018

How to Make the Mesenchymal Stem Cells Therapy More Targeted, More Accurate, and More Efficient?

J Craniofac Surg 2019 May/Jun;30(3):957-958

Institute of Biomedical Engineering, Chinese Academy of Medical Science & Peking Union Medical College; Logistics University of Chinese People's Armed Police Forces, Tianjin, China Department of Neurosurgery, Affiliated Hospital of Logistics University of Chinese People's Armed Police Forces, Tianjin, China State Key Laboratory of Precision Measurement Technology and Instruments, Tianjin University, Tianjin, China State Key Laboratory of Precision Measurement Technology and Instruments, Tianjin University, Tianjin, China Department of Radiotherapy of the Tianjin General Hospital, Tianjin, China State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Disease Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, China Institute of Biomedical Engineering, Chinese Academy of Medical Science & Peking Union Medical College Tech, Tianjin, China.

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http://dx.doi.org/10.1097/SCS.0000000000004822DOI Listing
October 2019

Expression of VEGF, COX-2 and MMP-9 in breast cancer and their relationship with ultrasound findings.

Int J Clin Exp Pathol 2018 1;11(9):4264-4269. Epub 2018 Sep 1.

Department of Breast Surgery, The Affiliated Hospital of Taishan Medical University Tai'an, Shandong Province, China.

Objective: We wished to ascertain the relationship between expression of vascular endothelial growth factor (VEGF), cyclo-oxygenase (COX)-2, and matrix metallopeptidase (MMP)-9 and various features of ultrasound images in breast cancer (BC) patients.

Methods: Eighty-nine breast lesions were confirmed to have BC by ultrasound, surgery and pathology. According to the Breast Imaging Reporting and Data System classification method published by the American College of Radiology, six groups were created: spiculation and non-Burr sign; calcification and non-calcification; vascular anomaly syndrome and non-vascular anomaly syndrome; lymph node metastasis (LNM) and non-LNM. In each case, the expression of VEGF, COX-2 and MMP-9 was detected by immunohistochemistry.

Results: Expression of VEGF, COX-2 and MMP-9 was higher in BC patients with the Burr sign than in those without it (P<0.05). There was no significant difference in expression of VEGF, COX-2, and MMP-9 between the calcification group and non-calcification group of BC patients (P>0.05). Expression of VEGF, COX-2, and MMP-9 was higher in those with vascular abnormalities than in those without them (P<0.05), as well as in the LNM group compared with the non-LNM group (P<0.05).

Conclusions: The high expression of VEGF, COX-2, and MMP-9 could have an influence on the Burr sign, abnormal blood vessels, and LNM in BC patients using ultrasound.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6962978PMC
September 2018

Metabolic profiles in community-acquired pneumonia: developing assessment tools for disease severity.

Crit Care 2018 05 14;22(1):130. Epub 2018 May 14.

Department of Respiratory and Critical Care Medicine, Peking University People's Hospital, Beijing, 100044, China.

Background: This study aimed to determine whether community-acquired pneumonia (CAP) had a metabolic profile and whether this profile can be used for disease severity assessment.

Methods: A total of 175 individuals including 119 CAP patients and 56 controls were enrolled and divided into two cohorts. Serum samples from a discovery cohort (n = 102, including 38 non-severe CAP, 30 severe CAP, and 34 age and sex-matched controls) were determined by untargeted ultra-high-performance liquid chromatography with tandem mass spectrometry (LC-MS/MS)-based metabolomics. Selected differential metabolites between CAP patients versus controls, and between the severe CAP group versus non-severe CAP group, were confirmed by targeted mass spectrometry assays in a validation cohort (n = 73, including 32 non-severe CAP, 19 severe CAP and 22 controls). Pearson's correlation analysis was performed to assess relationships between the identified metabolites and clinical severity of CAP. The area under the curve (AUC), sensitivity and specificity of the metabolites for predicting the severity of CAP were also investigated.

Results: The metabolic signature was markedly different between CAP patients and controls. Fifteen metabolites were found to be significantly dysregulated in CAP patients, which were mainly mapped to the metabolic pathways of sphingolipid, arginine, pyruvate and inositol phosphate. The alternation trends of five metabolites among the three groups including sphinganine, p-Cresol sulfate, dehydroepiandrosterone sulfate (DHEA-S), lactate and L-arginine in the validation cohort were consistent with those in the discovery cohort. Significantly lower concentrations of sphinganine, p-Cresol sulfate and DHEA-S were observed in CAP patients than in controls (p < 0.05). Serum lactate and sphinganine levels were positively correlated with confusion, urea level, respiratory rate, blood pressure, and age > 65 years (CURB-65), pneumonia severity index (PSI) and Acute Physiology and Chronic Health Evaluation II (APACHE II) scores, while DHEA-S inversely correlated with the three scoring systems. Combining lactate, sphinganine and DHEA-S as a metabolite panel for discriminating severe CAP from non-severe CAP exhibited a better AUC of 0.911 (95% confidence interval 0.825-0.998) than CURB-65, PSI and APACHE II scores.

Conclusions: This study demonstrates that serum metabolomics approaches based on the LC-MS/MS platform can be applied as a tool to reveal metabolic changes during CAP and establish a metabolite signature related to disease severity.

Trial Registration: ClinicalTrials.gov, NCT03093220 . Registered retrospectively on 28 March 2017.
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http://dx.doi.org/10.1186/s13054-018-2049-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5952829PMC
May 2018

Plan quality and robustness in field junction region for craniospinal irradiation with VMAT.

Phys Med 2018 Apr 23;48:21-26. Epub 2018 Mar 23.

Department of Biomedical Engineering, Tianjin University, 92 Weijin Road, Nankai District, Tianjin 300072, China. Electronic address:

Purpose: To propose a "staggered overlap" technique in volumetric modulated arc therapy (VMAT) for craniospinal irradiation (CSI) and compare the dose distribution and plan robustness with "overlap" technique and "gradient optimization" approach.

Methods And Materials: 6 patients previously treated in our clinic were retrospectively selected. 9 VMAT plans of each patient were optimized with "staggered overlap", "overlap" and "gradient optimization" in overlapping region of 3 cm, 6 cm, and 9 cm separately. For the "staggered overlap" plan, adjacent field sets were intentionally overlapped by staggering field edges in an appropriate step size to avoid sharp dose gradient. Evaluation metrics including V, D, D conformity number (CN) and homogeneity index (HI) were employed to evaluate the dose distribution. Moreover, shifts of the upper spinal field isocenter in each direction were performed to simulate junction errors for robustness analysis.

Results: The CN and HI of VMAT plans with "staggered overlap" were 0.82 (0.811-0.822) and 0.113 (0.112-0.114), while they were 0.778 (0.776-0.782) and 0.131 (0.130-0.131) for plans with "gradient optimization". In the robustness study, <3% dose deviations were found for 5 mm shifts in lateral and vertical directions with all techniques. In cranial-caudal direction, "overlap" technique created hot spots (D > 170%) and cold spots (D < 44%) in the junction region with 10 mm shifts. The dose deviations were decreased to 22% for plans with "staggered overlap" and 9 cm overlapping region.

Conclusion: "Staggered overlap" technique provides better plan quality as compared to "gradient optimization" approach and makes the plan more robust against junction errors as compared to "overlap" technique.
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http://dx.doi.org/10.1016/j.ejmp.2018.03.007DOI Listing
April 2018

Fine-Tuning Neural Patient Question Retrieval Model with Generative Adversarial Networks.

Stud Health Technol Inform 2018 ;247:720-724

IBM Research, China, Beijing.

The online patient question and answering (Q&A) system attracts an increasing amount of users in China. Patient will post their questions and wait for doctors' response. To avoid the lag time involved with the waiting and to reduce the workload on the doctors, a better method is to automatically retrieve the semantically equivalent question from the archive. We present a Generative Adversarial Networks (GAN) based approach to automatically retrieve patient question. We apply supervised deep learning based approaches to determine the similarity between patient questions. Then a GAN framework is used to fine-tune the pre-trained deep learning models. The experiment results show that fine-tuning by GAN can improve the performance.
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June 2018

Rhinovirus is associated with severe adult community-acquired pneumonia in China.

J Thorac Dis 2017 Nov;9(11):4502-4511

Department of Respiratory and Critical Care Medicine, Peking University People's Hospital, Beijing 100044, China.

Background: Human rhinovirus (HRV) is one of the most common viral etiologies detected in community-acquired pneumonia (CAP) adult cases. However, few is known about the characteristics of HRV-associated CAP. To describe the clinical features of HRV-associated CAP in immunocompetent adults admitted to multiple medical centers in mainland China over a 2-year period.

Methods: A total of 383 patients admitted to hospitals for CAP were enrolled from 46 medical centers in mainland China between January 2013 and December 2014. Multiplex real-time polymerase chain reaction (RT-PCR) assays for viral detection and DNA-based quantitative loop-mediated isothermal amplification (qLAMP) assays for bacterial detection were implemented to all lower respiratory tract specimens obtained from the patients. Twenty-eight cases (28/383, 7.3%) revealed HRV-positive PCR results. Patients with bronchoalveolar lavage (BAL) HRV-positive PCR results (n=20) were further enrolled and divided into two groups depending on the status of bacterial co-infection (viral group, n=12; viral-Bacterial group, n=8). Demographic, clinical and microbiological data were reviewed and compared in detail.

Results: Cases with HRV-infection were remarkably correlated with respiratory failure (14/20) and most of them (13/14) received mechanical ventilation. Fever (17/20), productive cough (15/20) and dyspnea (6/20) were common symptoms while flu-like symptoms were rarely observed in the cohort. (3/8), (3/8) and (2/8) were most frequently identified bacterium in the viral-bacterial group. Compared with the viral group, higher incidence of septic shock (3/8 1/12, P=0.255), longer ICU length of stay (LOS) (10.0 6.5 days, P=0.686), longer hospital LOS (18.5 13.0 days, P=0.208) and higher 28-day mortality (2/8 2/12, P=1) were observed in the Viral-Bacterial group, although without statistically significant difference.

Conclusions: HRV is a common etiology in CAP among China adults, especially in severe CAP. Clinicians should be vigilant considering of the poor outcome. Highly qualified multiplex PCR techniques with invasive sampling are needed to increase the detection rate.
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http://dx.doi.org/10.21037/jtd.2017.10.107DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5721016PMC
November 2017

C-erbB-2 expression is related with pathological progression of gastric cancer: results of a non-radioactive in situ hybridization.

Int J Clin Exp Pathol 2017 1;10(9):9649-9653. Epub 2017 Sep 1.

Department of General Surgery, Affiliated Hospital of Taishan Medical University Tai'an, Shandong Province, China.

Objective: To study the relationship of c-erbB-2 oncogene expression with major pathological characteristics of gastric cancer (GC) progression.

Methods: Eighty-one GC specimens were studied for c-erbB-2 oncogene amplification using non-radioactive in situ hybridization method. The c-erbB-2 overexpression status was correlated with tumor differentiation, tumor invasion and lymph node metastasis.

Results: Among the 81 pathology confirmed GC patients, 41 (50.6%) were found to have c-erbB-2 overexpression in cancer tissues. The rate of c-erbB-2 overexpression was significantly higher in those with poor tumor differentiation (63.0%, 29/46) than in those with well differentiated tumor (34.3%, 12/35) ( =6.576, P<0.001); significantly higher in those that invaded into deep muscle and beyond (55.7%, 39/70) than in those with tumors limited to the superficial muscle (18.2%, 2/11) ( =5.357, P<0.025); and significantly higher in those with lymph node metastases (59.6%, 34/57) than in those without lymph node involvement (29.2%, 7/24) ( =6.278, P<0.025).

Conclusions: c-erbB-2 oncogene overexpression may indicate a more aggressive biological behavior of the tumor and could be used as a predictive marker for GC pathological progression.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6965992PMC
September 2017

Inhibitory effect of the mitogen activated protein kinase specific inhibitor PD98059 on Mtb-Ag-activated γδΤ cells.

Int J Clin Exp Pathol 2017 1;10(9):9644-9648. Epub 2017 Sep 1.

Department of Breast Surgery, The Affiliated Hospital of Taishan Medical University Tai'an, Shandong Province, China.

Objective: This study aimed to observe the inhibitory effects of mitogen activated protein kinase (MAPK/ERK) specific inhibitor PD98059 on Mtb-Ag activated γδΤ cells and to investigate the role of MAPK in MAPK/ERK pathway of γδΤ cells activated by Mtb-Ag.

Methods: Healthy human peripheral blood mononuclear cell (PBMC) was isolated from the peripheral blood, and then stimulated by phorbol esters (PMA), ionomycin (IM) and Mtb-Ag. PBMC of the experimental group was pretreated by PD98059 of 0, 1, 10 or 100 μmol/L while the control group was given no pretreatment. Flow cytometry was applied to detect the expression of CD69 in γδΤ cells at 0 h, 6 h, 12 h, 24 h, 48 h and 72 h after treatment respectively. The influence of PD98059 on the amplification of γδΤ cells was also detected.

Results: After stimulated by PMA and IM for 6 hours, the expression of CD69 in γδΤ reached the peak of (99.3±1.09)%, while in the Mtb-Ag stimulation group, it reached the highest level of 75.2% at 24 hours. The level of CD69 in two group was significant difference between the two groups at each time respectively (p<0.05). Pretreated with PD98059 of 0, 1, 10, 100 μmol/L, the level of CD 69 expression in Mtb-Ag stimulated γδΤ was 79±0.8%, 75±0.7%, 54±0.5% and 17±0.2% respectively; In PMA+IM stimulated group, CD69 expression were all more than 98% in different concentration of PD98059. After treated with PD98059 of 0, 1, 10, 100 μmol/L, total cell number increased from 1.5×10 to (10.3±2.5)×10, (9.5±2.1)×10, (5.8±1.8)×10 and (2.1±0.5)×10 respectively. Number of γδΤ cells reached to (6.2±0.9)×10, (5.02±0.8)×10, (2.05±0.5)×10 and (0.41±0.1)×10 respectively.

Conclusion: γδΤ cells were specifically activated by Mtb-Ag, and the activation was depended on MAPK/ERK pathway. The activation effects of Mtb-Ag on γδΤ cells can be obviously inhibited by PD98059.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6965926PMC
September 2017

Timing for intracoronary administration of bone marrow mononuclear cells after acute ST-elevation myocardial infarction: a pilot study.

Stem Cell Res Ther 2015 May 29;6:112. Epub 2015 May 29.

Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, 180 Feng Lin Road, Shanghai, 200032, China.

Introduction: Most studies on intracoronary bone marrow mononuclear cell transplantation for acute myocardial infarction involve treatment 3-7 days after primary percutaneous coronary intervention (PCI); however, the optimal timing is unknown. The present study assessed the therapeutic effect at different times after ST-elevation myocardial infarction.

Methods: The present trial was not blinded. A total of 104 patients with a first ST-elevation myocardial infarction and a left ventricular ejection fraction below 50 %, who had PCI of the infarct-related artery, were randomly assigned to receive intracoronary infusion of bone marrow mononuclear cells within 24 hours (group A, n = 27), 3 to 7 days after PCI (group B, n = 26), or 7 to 30 days after PCI (group C, n = 26), or to the control group (n = 25), which received saline infusion performed immediately after emergency PCI. All patients in groups A, B and C received an injection of 15 ml cell suspension containing approximately 4.9 × 10(8) bone marrow mononuclear cells into the infarct-related artery after successful PCI.

Results: Compared to control and group C patients, group A and B patients had a significantly higher absolute increase in left ventricular ejection fraction from baseline to 12 months (change: 3.4 ± 5.7 % in control, 7.9 ± 4.9 % in group A, 6.9 ± 3.9 % in group B, 4.7 ± 3.7 % in group C), a greater decrease in left ventricular end-systolic volumes (change: -6.4 ± 15.9 ml in control, -20.5 ± 13.3 ml in group A, -19.6 ± 11.1 ml in group B, -9.4 ± 16.3 ml in group C), and significantly greater myocardial perfusion (change from baseline: -4.7 ± 5.7 % in control, -7.8 ± 4.5 % in group A, -7.5 ± 2.9 % in group B, -5.0 ± 4.0 % in group C). Group A and B patients had similar beneficial effects on cardiac function (p = 0.163) and left ventricular geometry (left ventricular end-distolic volume: p = 0.685; left ventricular end-systolic volume: p = 0.622) assessed by echocardiography, whereas group C showed similar results to those of the control group. Group B showed more expensive care (p < 0.001) and longer hospital stays during the first month after emergency PCI (p < 0.001) than group A, with a similar improvement after repeat cardiac catheterization following emergency PCI.

Conclusion: Cell therapy in acute myocardial infarction patients that is given within 24 hours is similar to 3-7 days after the primary PCI.

Trial Registration: NCT02425358 , registered 30 April 2015.
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http://dx.doi.org/10.1186/s13287-015-0102-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4509778PMC
May 2015

[The clinical-radiologic-pathologic features of imported pulmonary histoplasmosis].

Zhonghua Jie He He Hu Xi Za Zhi 2015 Jan;38(1):23-8

Email:

Objective: To describe the clinical features and treatment of imported pulmonary histoplasmosis and therefore to improve the recognition and differential diagnosis of this disease.

Methods: The clinical data of 3 patients with imported pulmonary histoplasmosis in our hospital were collected and analyzed. Literatures published since 1989 were retrieved with 'pulmonary histoplasmosis' from PubMed, China National Knowledge Infrastructure (CNKI), Wanfang Data and VIP data, of which all the literatures about imported pulmonary histoplasmosis were reviewed. The clinical manifestations, diagnostic methods and treatment were summarized.

Results: All the 3 cases of imported pulmonary histoplasmosis were immunocompetent hosts, all were males, age were from 44-67 years, and had a history of exploring the cave or tunnel inhabited by bats in the epidemic areas. All of them developed influenza-like symptoms varying in severity after the onset of the disease. Pulmonary multiple nodules and mediastinal lymphadenopathy were found on chest images. One patient underwent percutaneous lung biopsy and the other two received video-assisted thoracoscopic lung biopsy. All the 3 patients showed consistent histopathological findings, such as granulomatous inflammation with necrosis. Pathogen culture with lung biopsy in the first case was identified as histoplasma. All the 3 cases were treated with itraconazole, and recovered with good prognosis. Thirteen literatures in English were obtained, which reported 60 cases with imported pulmonary histoplasmosis. Forty-two of them were males, 16 were females and 2 undefined. The range of their age was from 17-64 years. No imported pulmonary histoplasmosis was reported so far in Chinese literature. Common features of imported pulmonary histoplasmosis were consistent with our patients, including epidemiology, influenza-like symptoms and bilateral pulmonary nodules, recovery with or without antifungal therapy.

Conclusion: The epidemiologic history, influenza-like symptoms and bilateral pulmonary nodules provide valuable diagnostic clues for imported histoplasmosis. Clinical features with pathologic findings and good response to antifungal therapy could make the diagnosis even without pathogen detection if other etiology is unlikely.
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January 2015

Probucol Protects Against Atherosclerosis Through Lipid-lowering and Suppressing Immune Maturation of CD11c+ Dendritic Cells in STZ-induced Diabetic LDLR-/- Mice.

J Cardiovasc Pharmacol 2015 Jun;65(6):620-7

*Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai, China; †Department of cardiology, Huashan Hospital, Fudan University, Shanghai, China; ‡Institute of Biomedical Science, Fudan University, Shanghai, China; and §Institute of Cardiovascular Sciences and Key Laboratory of Molecular Cardiovascular Sciences, Ministry of Education, Peking University, Beijing, China.

Probucol, an agent characterized by lipid-lowering and antioxidant property, retards atherosclerosis effectively. To test the hypothesis that probucol might act its antiatherosclerotic role by suppressing immune maturation of dendritic cells (DCs), 7-week-old LDLR mice were rendered diabetic with streptozotocin (STZ) and then fed either a high-fat diet only or added with 0.5% (wt/wt) probucol for 4 months, and human monocyte-derived dendritic cells were preincubated with or without probucol and stimulated by oxidized low-density lipoprotein. In STZ-induced diabetic LDLR mice, probucol treatment significantly lowered plasma total cholesterol and high-density lipoprotein-cholesterol levels; regressed aortic atherosclerotic lesions; reduced splenic CD40, CD80, CD86, MHC-II expression, and plasma IL-12p70 production; and decreased the expression of CD11c DCs within atherosclerotic lesions. In vitro, oxidized low-density lipoprotein promoted human monocyte-derived dendritic cells maturation; stimulated CD40, CD86, CD1a, HLA-DR expression; increased tumor necrosis factor-α production; and decreased IL-4 production. However, these effects were obviously inhibited by probucol pretreatment. In conclusion, our study indicated that probucol effectively retarded atherosclerosis at least partly through lipid-lowering and inhibiting immune maturation of CD11c DCs in STZ-induced diabetic LDLR mice.
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http://dx.doi.org/10.1097/FJC.0000000000000234DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4461394PMC
June 2015

[Expression kinetics of CD69 molecule by CD3⁺ lymphocytes and γδT cells under three different activating modalities].

Zhonghua Xue Ye Xue Za Zhi 2014 Aug;35(8):753-4

Department of Clinical Laboratory, Affiliated Hospital of Taishan Medical College, Shandong Taian 271000, China.

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http://dx.doi.org/10.3760/cma.j.issn.0253-2727.2014.08.020DOI Listing
August 2014

Geometrical and volume changes of the membranous vestibular labyrinth in guinea pigs with endolymphatic hydrops.

ORL J Otorhinolaryngol Relat Spec 2013 27;75(2):108-16. Epub 2013 Jun 27.

Eye & ENT Hospital, Fudan University, Shanghai, China.

Objective: To evaluate geometrical and volume changes of membranous vestibular labyrinths in guinea pigs after endolymphatic hydrops (EH).

Methods: The membranous labyrinths of normal guinea pigs and of those with EH for 4 and 8 weeks were reconstructed after being scanned using micro-computed tomography subseqent to being stained in osmium tetroxide (OsO4). The diameters and volumes of the semicircular ducts, ampullae, utricles and saccules were measured based on the three-dimensional models.

Results: The diameters of the ampullae and utricles of EH guinea pigs were greater than those of the normal guinea pigs, while there were no significant differences in the diameters of the semicircular ducts among all groups. The volumes of ampullae, utricles and saccules of the EH groups were greater than those of the control group, but there were no changes in volumes of semicircular ducts after EH.

Conclusion: The dilations of the membranous vestibular labyrinth in guinea pigs with EH mainly occur at the ampullae, utricles and saccules.
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http://dx.doi.org/10.1159/000350569DOI Listing
March 2014

Exogenous taurine attenuates mitochondrial oxidative stress and endoplasmic reticulum stress in rat cardiomyocytes.

Acta Biochim Biophys Sin (Shanghai) 2013 May;45(5):359-67

Department of Cellular and Genetic Medicine, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China.

Taurine, a conditionally essential amino acid, plays a critical role in cardiovascular function. Here we examined the effect of taurine on mitochondria and endoplasmic reticulum in rat cardiomyocytes during glucose deprivation (GD). Data showed that cell viability, intracellular taurine contents, and taurine transporter expression were decreased during GD. In contrast, an increase in reactive oxygen species and intracellular Ca(2+) contents was observed. GD also caused disrupted mitochondrial membrane potential, apoptotic cell death, and dissociation of unfolded protein response (UPR)-relative proteins in cardiomyocytes. Signal transduction analysis showed that Bcl-2 family protein balance was disturbed, caspase-12 was activated and UPR-relative protein levels were up-regulated. Moreover, pre-treatment with 80 mM exogenous taurine attenuated GD effect in cardiomyocytes. Our results suggest that taurine have beneficial effects on inhibiting mitochondria-dependent cell apoptosis and UPR-associated cell apoptosis and might have clinical implications on acute myocardial infarction in future.
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http://dx.doi.org/10.1093/abbs/gmt034DOI Listing
May 2013

Naoxintong protects against atherosclerosis through lipid-lowering and inhibiting maturation of dendritic cells in LDL receptor knockout mice fed a high-fat diet.

Curr Pharm Des 2013 ;19(33):5891-6

Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, 180 Fenglin Rd, Shanghai 200032,PR China. ;

Naoxintong (NXT), a Chinese Materia Medica standardized product, extracted from 16 various kinds of Chinese traditional herbal medicines including Salvia miltiorrhiza, Angelica sinennsis, Astragali Radix, is clinically effective in treating atherosclerosisrelated diseases. Here, we tested the hypothesis that the anti-atherosclerosis effects of NXT might be mediated by suppressing maturation of dendritic cells (DCs) in a mice model of atherosclerosis. LDLR(-/-) mice fed a high-fat diet were treated with placebo, NXT (0.7 g/kg/d, oral diet) or simvastatin (100mg/kg/d, oral diet) for 8 weeks, respectively. NXT treatment significantly reduced plasma triglyceride (112 ± 18 mg/dl vs. 192 ± 68 mg/dl, P<0.05) and total cholesterol (944 ± 158 mg/dl vs. 1387 ± 208 mg/dl, P<0.05) compared to placebo treatment. Vascular lesions were significantly smaller and macrophage content and amount of DCs in plaques were significantly less in NXT and simvastatin groups than in placebo group (all P<0.05). In addition, expressions of splenic DC membrane molecules (CD40, CD86 and CD80) and the plasma level of IL-12p70 were significantly lower in NXT and simvastatin groups than in placebo group. In conclusion, NXT protects against atherosclerosis through lipid-lowering and inhibiting DCs maturation in this mice model of atherosclerosis.
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http://dx.doi.org/10.2174/1381612811319330008DOI Listing
April 2014

Taurine detected using high-resolution magic angle spinning (1)H nuclear magnetic resonance: A potential indicator of early myocardial infarction.

Exp Ther Med 2013 Mar 13;5(3):683-688. Epub 2012 Dec 13.

Zhongshan Hospital of Fudan University, Shanghai Institute of Cardiovascular Disease, Shanghai 200032; ; Department of Cellular & Genetic Medicine, Shanghai Medical School, Fudan University, Shanghai 200032;

Magnetic resonance spectroscopy (MRS) is a unique non-invasive method for detecting cardiac metabolic changes. However, MRS in cardiac diagnosis is limited due to insensitivity and low efficiency. Taurine (Tau) is the most abundant free amino acid in the myocardium. We hypothesized that Tau levels may indicate myocardial ischemia and early infarction. Sprague-Dawley rats were divided into seven groups according to different time points during the course of myocardial ischemia, which was induced by left anterior descending coronary artery ligation. Infarcted myocardial tissue was obtained for high-resolution magic angle spinning (1)H nuclear magnetic resonance (NMR) analysis. Results were validated via high-performance liquid chromatography. The Tau levels in the ischemic myocardial tissue were reduced significantly within 5 min compared with those in the control group (relative ratio from 20.27±6.48 to 8.81±0.04, P<0.05) and were maintained for 6 h post-ischemia. Tau levels declined more markedly (56.5%) than creatine levels (48.5%) at 5 min after ligation. This suggests that Tau may have potential as an indicator in the early detection of myocardial ischemia by (1)H MRS.
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http://dx.doi.org/10.3892/etm.2012.857DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3570233PMC
March 2013

Up-regulation of BMP-2 antagonizes TGF-β1/ROCK-enhanced cardiac fibrotic signalling through activation of Smurf1/Smad6 complex.

J Cell Mol Med 2012 Oct;16(10):2301-10

Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital and Institutes of Biomedical Sciences, Fudan University, Shanghai, China.

Rho-associated kinase (ROCK) plays a critical role in pressure overload-induced left ventricular remodelling. However, the underlying mechanism remains unclear. Here, we reported that TGF-β1-induced ROCK elevation suppressed BMP-2 level and strengthened fibrotic response. Exogenous BMP-2 supply effectively attenuated TGF-β1 signalling pathway through Smad6-Smurf-1 complex activation. In vitro cultured cardiomyocytes, mechanical stretch up-regulated cardiac TGF-β1, TGF-β1-dependent ROCK and down-regulated BMP-2, but BMP-2 level could be reversed through blocking TGF-β1 receptor by SB-431542 or inhibition of ROCK by Y-27632. TGF-β1 could also activate ROCK and suppress endogenous BMP-2 level in a dose-dependent manner. Knock-down BMP-2 enhanced TGF-β1-mediated PKC-δ and Smad3 signalling cascades. In contrast, treatment with Y-27632 or SB-431542, respectively suppressed ROCK-dependent PKC-δ and Smad3 activation, but BMP-2 was only up-regulated by Y-27632. In addition, BMP-2 silencing abolished the effect of Y-27632, but not SB-431542 on suppression of TGF-β1 pathway. Further experiments showed that Smad6 Smurf1 interaction were required for BMP-2-evoked antagonizing effects. Smad6 overexpression attenuated TGF-β1-induced activation of PKC-δ and Smad3, promoted TGF-β RI degradation in BMP-2 knock-down cardiomyocytes, and could be abolished after knocking-down Smurf-1, in which Smad6/Smurf1 complex formation was critically involved. In vivo data showed that pressure overload-induced collagen deposition was attenuated, cardiac function was improved and TGF-β1-dependent activation of PKC-δ and Smad3 was reduced after 2 weeks treatment with rhBMP-2(0.5 mg/kg) or Y-27632 (10 mg/kg) in mice that underwent surgical transverse aortic constriction. In conclusion, we propose that BMP-2, as a novel fibrosis antagonizing cytokine, may have potential beneficial effect in attenuating pressure overload-induced cardiac fibrosis.
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http://dx.doi.org/10.1111/j.1582-4934.2012.01538.xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3823423PMC
October 2012

[Relationship between 3-d reconstruction of regenerated fibers and functional recovery after mastoid segment of facial nerve was repaired].

Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 2011 Oct;25(19):895-8

Department of Anatomy, Histology and Embryology, Shanghai Medical College of Fudan University, Shanghai.

Objective: To explore the relationship between three-dimensional (3-D) reconstruction of regenerated fibers and functional recovery after mastoid segment of facial nerve was repaired with either end-to-end anastomosis or autogenous great auricular nerve grafting.

Method: Thirty healthy adult New Zealand white rabbits were randomly divided into two groups: one was end-to-end anastomosis group and another was autogenous great auricular nerve grafting group. Only mastoid segment of right facial nerve of each animal was dissected and the contralateral nerve was as a control. Electromyogram (EMG) were recorded from 5 animals of each group at the 30th d, 90th d and 120th d after operation. After that the nerves were extracted, fixed, decalcified and embedded in paraffin. Samples was sectioned serially at 6 microm and stained with special trichrome stain. All the imagines were imported into Mimics software to reconstruct the 3-D model.

Result: The significant differences were found in the regenerate fibers on 30 th d,and were found in amplitude of EMG on 30 th d and 90 th d. The image of 3-D reconstruction showed that the myelin sheath were thickening, connected from proximal to the distal gradually after repair.

Conclusion: The 3-D reconstruction of regenerated nerve fibers partly conformed to the functional recovery after facial nerve trunk was repaired. The functional recovery of facial nerve was related with both the quantity and the quality of regenerated nerve fibers.
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October 2011
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