Publications by authors named "Kennia Scapin Viola"

3 Publications

  • Page 1 of 1

Physicochemical, biological, and antibacterial evaluation of tricalcium silicate-based reparative cements with different radiopacifiers.

Dent Mater 2021 02 13;37(2):311-320. Epub 2020 Dec 13.

Department of Restorative Dentistry, São Paulo State University (UNESP), School of Dentistry, Araraquara, SP, Brazil. Electronic address:

Objective: To evaluate tricalcium silicate-based (TCS) experimental materials, associated with zirconium oxide (ZrO), calcium tungstate (CaWO) or niobium oxide (NbO) radiopacifiers, in comparison with MTA Repair HP (Angelus).

Methods: Physicochemical tests: setting time, radiopacity, pH and solubility. In vitro assays: cytotoxicity: MTT and Neutral Red - NR; cell bioactivity: alkaline phosphatase activity (ALP), Alzarin red staining (ARS) and real time PCR (qPCR). Antibacterial activity: direct contact on Enterococcus faecalis in the planktonic form. Physicochemical and ARS data were submitted to ANOVA/Tukey tests; antibacterial activity, to Kruskall-Wallis and Dunn tests; MTT, NR, ALP and qPCR were analyzed by ANOVA/Bonferroni tests (α = 0.05).

Results: TCS + CaWO presented the longest setting time and MTA HP the shortest. Except for TCS, all the materials presented radiopacity above 3 mmAl. The cements had alkaline pH, antibacterial activity, low solubility and no cytotoxic effects. The highest ALP activity occurred in 14 days, especially to TCS, TCS + ZrO and TCS + CaWO. TCS + ZrO, TCS + NbO and MTAHP had higher mineralized nodule formation than those of the negative control (NC). After 7 days, there was no difference in mRNA expression for ALP, when compared to NC. However, after 14 days there was no overexpressed ALP mRNA, especially TCS + NbO, in relation to the CN. All the materials presented antimicrobial action.

Significance: The pure tricalcium silicate associated with ZrO, CaWO or NbO had appropriate physicochemical properties, antibacterial activity, cytocompatibility and induced mineralization in Saos-2, indicating their use as reparative materials.
View Article and Find Full Text PDF

Download full-text PDF

Source Listing
February 2021

In vivo and in vitro anti-inflammatory and pro-osteogenic effects of citrus cystatin CsinCPI-2.

Cytokine 2019 11 18;123:154760. Epub 2019 Jun 18.

Department of Diagnosis and Surgery, School of Dentistry at Araraquara, Sao Paulo State University - UNESP, Araraquara, São Paulo, Brazil. Electronic address:

Cystatins are natural inhibitors of cysteine peptidases. Recently, cystatins derived from plants, named phytocystatins, have been extensively studied. Among them, CsinCPI-2 proteins from Citrus sinensis were identified and recombinantly produced by our group. Thus, this study described the recombinant expression, purification, and inhibitory activity of this new phytocystatin against human cathepsins K and B and assessed the anti-inflammatory effect of CsinCPI-2 in vitro in mouse and in vivo in rats. In addition, the pro-osteogenic effect of CsinCPI-2 was investigated in vitro. The inflammatory response of mouse macrophage cells stimulated with P. gingivalis was modulated by CsinCPI-2. The in vitro results showed an inhibitory effect (p < 0.05) on cathepsin K, cathepsin B, IL-1β, and TNF-α gene expression. In addition, CsinCPI-2 significantly inhibited in vivo the activity of TNF-α (p < 0.05) in the blood of rats, previously stimulated by E. coli lipopolysaccharide (LPS). CsinCPI-2 had a pro-osteogenic effect in human dental pulp cells, demonstrated by the increase in alkaline phosphatase (ALP) activity, deposition of mineralized nodules, and the gene expression of the osteogenic markers as bone morphogenetic protein 2 (BMP-2), runt-related transcription factor 2 (Runx-2), ALP, osteocalcin, and bone sialoprotein (BSP). These preliminary studies suggested that CsinCPI-2 has a potential anti-inflammatory, and at the same time, a pro-osteogenic effect. This may lead to new therapies for the control of diseases where inflammation plays a key role, such as periodontal disease and apical periodontitis.
View Article and Find Full Text PDF

Download full-text PDF

Source Listing
November 2019

Effect of rotary instrument associated with different irrigation techniques on removing calcium hydroxide dressing.

Microsc Res Tech 2014 Aug 20;77(8):642-6. Epub 2014 May 20.

Department of Restorative Dentistry, Araraquara Dental School, UNESP - Univ Estadual Paulista, Araraquara, SP, Brazil.

Calcium hydroxide [Ca(OH)2 ] residues in root canals may compromise sealing of filling and endodontic treatment success. The aim of this study was to compare the efficacy of using rotary instrument associated with EndoActivator, EndoVac, passive ultrasonic irrigation (PUI), and conventional needle irrigation (CNI), in Ca(OH)2 removal from root canal, by means of scanning electron microscopy (SEM) images. Sixty-six human canines were prepared with the Protaper system up to F5 and filled with Ca(OH)2 . After 7 days, Ca(OH)2 was removed with rotary instrument F5 associated with the irrigation techniques used in each group (n = 15): GI (CNI), GII (EndoVac), GIII (EndoActivator) and GIV (PUI). In all groups 15 mL of 2.5% NaOCl and 3 mL of 17% EDTA were used for Ca(OH)2 removal. The Ca(OH)2 residues was evaluated by SEM in the middle and apical third using a system of scores. The results were analyzed by the Kruskal-Wallis and Dunn tests (α = 0.05). None of the techniques completely removed the Ca(OH)2 from root canals. There was no difference between EndoActivator, EndoVac and PUI (P > 0.05), but the three techniques removed more Ca(OH)2 than the CNI (P < 0,05), in the middle and apical thirds of the root canal. It was concluded that the rotary instrument combined with EndoActivator, EndoVac, and PUI was shown to be more efficient than the rotary instrument combined with the CNI in removing Ca(OH)2 from the root canal.
View Article and Find Full Text PDF

Download full-text PDF

Source Listing
August 2014