Publications by authors named "Kefu Yu"

100 Publications

Association between SNPs and hepatotoxicity in patients with primary central nervous system lymphoma on high-dose methotrexate therapy.

J Pharm Pharmacol 2021 Jul 13. Epub 2021 Jul 13.

Department of Pharmacy, Beijing Tiantan Hospital, Capital Medical University, Beijing, P.R. China.

Objectives: This study aims to evaluate the association between polymorphisms of methotrexate pathway genes and high-dose methotrexate-related hepatotoxicity in Chinese patients with primary central nervous system lymphoma.

Methods: Sixty-five patients in 411 treatment courses were enrolled and their toxicities were evaluated. The association between 30 candidate SNPs from 20 methotrexate pathway genes and high-dose methotrexate-related hepatotoxicity was analysed by PLINK and logistic regression.

Key Findings: TYMS 6 bp DI + II (rs151264360; OR, 0.41; 95% CI, 0.25-0.66; P = 0.00029), MTHFD1 1958 GA + AA (rs2236225; OR, 0.55; 95% CI, 0.33-0.91; P = 0.020) and CCND1 870 GA + GG (rs9344; OR, 0.42; 95% CI, 0.24-0.73; P = 0.0024) had less risk of hepatotoxicity compared with their homozygotes (DD, GG and AA, respectively), while ABCC2 intron 29 GA + GG (rs3740065; OR, 3.14; 95% CI, 1.89-5.20; P = 0.00001) was more prevalent in patients with hepatotoxicity than TT.

Conclusions: TYMS 6 bp DI + II, MTHFD1 1958 GA + AA, CCND1 870 GA + GG genotypes were associated with a lower probability of hepatotoxicity in patients with primary central nervous system lymphoma on high-dose methotrexate therapy, and ABCC2 intron 29 GA + GG was correlated with increased risk of hepatotoxicity.
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http://dx.doi.org/10.1093/jpp/rgab099DOI Listing
July 2021

Seasonal fluctuations in symbiotic bacteria and their role in environmental adaptation of the scleractinian coral Acropora pruinosa in high-latitude coral reef area of the South China Sea.

Sci Total Environ 2021 Jun 11;792:148438. Epub 2021 Jun 11.

Guangxi Laboratory on the Study of Coral Reefs in the South China Sea, Coral Reef Research Center of China, School of Marine Sciences, Guangxi University, Nanning, China.

Coral-associated bacterial communities are paramount for coral ecosystems and holobiont health. However, the role of symbiotic bacteria in the adaptation of high-latitude corals to seasonal fluctuations remains underexplored. Therefore, we used 16S rRNA-based high-throughput sequencing to analyze the symbiotic bacterial diversity, composition, and core bacterial community in high-latitude coral and explored the seasonal fluctuation characteristics of symbiotic bacterial communities. We found that bacterial richness and α-diversity changed significantly across different seasons. Additionally, the community structure recombined seasonally, with different dominant bacterial phyla and genera in different seasons. However, the symbiotic bacterial community structures of Acropora pruinosa in winter and spring were similar. Proteobacteria were the dominant bacteria in spring, autumn, and winter. In summer, the dominant bacterial taxa were Bacteroidota and Proteobacteria. Ralstonia was the dominant bacterial genus in spring and winter, whereas in autumn, BD1-7_clade was dominant. Linear discriminant analysis effect size identified 20 abundant genera between the different groups. Core microbiome analysis revealed that 12 core bacterial operational taxonomic units were associated with A. pruinosa in all seasons, seven of which varied with the seasons, changing between dominant and rare. Distance-based redundancy and variation partitioning analyses revealed that sea surface temperature was the major contributor of variation in the microbial community structure. We hypothesized that the high diversity and abundance of symbiotic bacteria and the increase in Prosthecochloris abundance in coral in summer can help A. pruinosa maintain its physiological functions, ameliorating the negative physiological effects of the decrease in Symbiodiniaceae density under high-temperature stress. Thus, the rapid reorganization of the symbiotic bacterial community structure and core microflora in different seasons may allow the corals to adapt to large seasonal environmental fluctuations. In conclusion, seasonal variation of bacteria plays an important role in coral adaptation to large environmental fluctuations.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148438DOI Listing
June 2021

Combining MGMT promoter pyrosequencing and protein expression to optimize prognosis stratification in glioblastoma.

Cancer Sci 2021 Jun 11. Epub 2021 Jun 11.

Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.

Pyrosequencing (PSQ) represents the golden standard for MGMT promoter status determination. Binary interpretation of results based on the threshold from the average of several CpGs tested would neglect the existence of the "gray zone". How to define the gray zone and reclassify patients in this subgroup remains to be elucidated. A consecutive cohort of 312 primary glioblastoma patients were enrolled. CpGs 74-81 in the promoter region of MGMT were tested by PSQ and the protein expression was assessed by immunohistochemistry (IHC). Receiver operating characteristic curves were constructed to calculate the area under the curves (AUC). Kaplan-Meier plots were used to estimate the survival rate of patients compared by the log-rank test. The optimal threshold of each individual CpG differed from 5% to 11%. Patients could be separated into the hypomethylated subgroup (all CpGs tested below the corresponding optimal thresholds, n = 126, 40.4%), hypermethylated subgroup (all CpGs tested above the corresponding optimal thresholds, n = 108, 34.6%), and the gray zone subgroup (remaining patients, n = 78, 25.0%). Patients in the gray zone harbored an intermediate prognosis. The IHC score instead of the average methylation levels could successfully predict the prognosis for the gray zone (AUC for overall survival, 0.653 and 0.519, respectively). Combining PSQ and IHC significantly improved the efficiency of survival prediction (AUC: 0.662, 0.648, and 0.720 for PSQ, IHC, and combined, respectively). Immunohistochemistry is a robust method to predict prognosis for patients in the gray zone defined by PSQ. Combining PSQ and IHC could significantly improve the predictive ability for clinical outcomes.
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http://dx.doi.org/10.1111/cas.15024DOI Listing
June 2021

Microbiome of juvenile corals in the outer reef slope and lagoon of the South China Sea: insight into coral acclimatization to extreme thermal environments.

Environ Microbiol 2021 Jun 10. Epub 2021 Jun 10.

Coral Reef Research Center of China, Guangxi University, Nanning, 530004, China.

Environmental conditions between the outer reef slope (ORS) and lagoon in tropical atolls are significantly different, but the variations of juvenile coral-microbiomes in the two environments and their relationship with coral thermal acclimatization are poorly understood. We explored this issue based on local water conditions and the microbiome of juvenile corals in the ORS and lagoon in the central South China Sea. Coral-symbiotic Symbiodiniaceae showed significant differences among coral species; Pocillopora verrucosa and Pachyseris rugosa in the ORS, and Acropora formosa in the lagoon were dominated by Durusdinium, but other corals were dominated by Cladocopium. Although A. formosa in the ORS were dominated by Cladocopium (C3u), they were dominated by Durusdinium (D1/D1a) and Cladocopium (C50) in the lagoon. Other coral species were both dominated by Cladocopium in the lagoon and ORS. The relative abundance of bacteria in the Deinococcus-Thermus was generally higher in the lagoon corals than in the ORS corals. Our study indicates that P. verrucosa, P. rugosa and Porites lutea may have high thermal tolerance based on the relatively high abundance of heat-tolerant Durusdinium and Thermus scotoductus. Likewise, A. formosa in the lagoon may acclimatize to the thermal environment based on a high relative abundance of heat-tolerant Durusdinium.
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http://dx.doi.org/10.1111/1462-2920.15624DOI Listing
June 2021

Corrigendum: Distinguishing Pseudoprogression From True Early Progression in Isocitrate Dehydrogenase Wild-Type Glioblastoma by Interrogating Clinical, Radiological and Molecular Features.

Front Oncol 2021 19;11:700599. Epub 2021 May 19.

Department of Neurosurgery, National Clinical Research Center for Neurological Diseases, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.

[This corrects the article DOI: 10.3389/fonc.2021.627325.].
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http://dx.doi.org/10.3389/fonc.2021.700599DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8171117PMC
May 2021

Differential Predictors and Clinical Implications Associated With Long-Term Survivors in IDH Wildtype and Mutant Glioblastoma.

Front Oncol 2021 13;11:632663. Epub 2021 May 13.

Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.

Background: Glioblastoma (GBM) is the most aggressive intracranial tumor which can be divided into two subtypes based on status of isocitrate dehydrogenase (IDH). A small fraction of patients after receiving standard treatment can be long-term survivors (LTS). This study was designed to disclose the predictors and clinical implications associated with LTS in IDH wildtype and mutant GBM.

Methods: Patients who survived beyond five years after diagnosis of GBM were defined as LTS, while those with a survival less than one year were defined as short-term survivors (STS). A total of 211 patients with diagnosis of GBM in Beijing Tiantan Hospital from January 2007 to January 2015 were enrolled, including 44 (20.9%) LTS and 167 (79.1%) STS. The clinical, radiological and molecular features between groups were systematically compared.

Results: Compared with STS, LTS were a subgroup of patients with a younger age at diagnosis (=0.006), a higher KPS score (=0.011), higher rates of cystic change (=0.037), O-methylguanine-DNA methyltransferase (MGMT) promoter methylation (=0.007), and IDH mutation (=0.049), and more likely to have undergone gross total resection (<0.001). Survival analysis demonstrated that LTS with wildtype IDH conferred a longer progression-free survival (66.0 27.0 months, =0.04), but a shorter post-progression survival (46.5 months not reached, =0.0001) than those of LTS with mutant IDH. LTS with mutant IDH showed a trend towards increased survival after receiving re-operation (=0.155) and reirradiation (=0.127), while this clinical benefit disappeared in the subset of LTS with wildtype IDH (>0.05).

Conclusion: The prognostic value and therapeutic implications associated with LTS in GBM population significantly differed on the basis of IDH status. Our findings provide a new approach for physicians to better understand the two subtypes of GBM, which may assist in making more tailored treatment decisions for patients.
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http://dx.doi.org/10.3389/fonc.2021.632663DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8155513PMC
May 2021

Combination of Immunotherapy and Radiotherapy for Recurrent Malignant Gliomas: Results From a Prospective Study.

Front Immunol 2021 7;12:632547. Epub 2021 May 7.

Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.

Background: World Health Organization (WHO) grade IV glioma remains one of the most lethal tumors with a dismal prognosis and inevitable recurrence. We evaluated the safety and efficacy of immunotherapy with radiotherapy in this population of patients.

Methods: This study was a single-arm, open-label, phase I trial based on patients with recurrent WHO grade IV glioma. Patients were treated with intracranial and systemic immunoadjuvants in combination with low-dose reirradiation. The primary endpoint of the present trial was safety. Secondary endpoints were overall survival (OS) and progression-free survival (PFS). This trial is registered at ClinicalTrials.gov, NCT03392545.

Results: Thirty patients were enrolled. The most common adverse events (AEs) were fever (66.7%), vomiting (33.3%), headache (30.0%), and fatigue (23.3%). Only a single patient experienced grade 3 fever, and no grade 4 AEs or deaths related to treatment were observed. Of the 30 patients, 1 (3.3%) had a complete response, 5 (16.7%) had a partial response, 9 (30.0%) had stable disease, and 15 (50.0%) had progressive disease, resulting in an objective response rate of 20.0%. The median PFS of the entire cohort was 88.0 (61.0-254.0) days, and the median OS was 362.0 (197.0-601.0) days. Patients could be divided into responders and non-responders, and these groups exhibited a significant difference in terms of survival time, T lymphocyte subsets, frequency of cell division cycle 27 (CDC27) mutation status, and CD15 and CD68 expression (<0.05).

Conclusion: The combination of immunotherapy and radiotherapy is well tolerated and may provide clinical benefit for patients with recurrent WHO grade IV glioma. A prospective phase II study is needed to further validate the efficacy of our therapeutic regimen.
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http://dx.doi.org/10.3389/fimmu.2021.632547DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8138184PMC
May 2021

Distinguishing Pseudoprogression From True Early Progression in Isocitrate Dehydrogenase Wild-Type Glioblastoma by Interrogating Clinical, Radiological, and Molecular Features.

Front Oncol 2021 20;11:627325. Epub 2021 Apr 20.

Department of Neurosurgery, National Clinical Research Center for Neurological Diseases, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.

Pseudoprogression (PsP) mimics true early progression (TeP) in conventional imaging, which poses a diagnostic challenge in glioblastoma (GBM) patients who undergo standard concurrent chemoradiation (CCRT). This study aimed to investigate whether perioperative markers could distinguish and predict PsP from TeP in isocitrate dehydrogenase (IDH) wild-type GBM patients. New or progressive gadolinium-enhancing lesions that emerged within 12 weeks after CCRT were defined as early progression. Lesions that remained stable or spontaneously regressed were classified as PsP, otherwise persistently enlarged as TeP. Clinical, radiological, and molecular information were collected for further analysis. Patients in the early progression subgroup were divided into derivation and validation sets (7:3, according to operation date). Among 234 consecutive cases enrolled in this retrospective study, the incidences of PsP, TeP, and neither patterns of progression (nP) were 26.1% (61/234), 37.6% (88/234), and 36.3% (85/234), respectively. In the early progression subgroup, univariate analysis demonstrated female (: 2.161, = 0.026), gross total removal (GTR) of the tumor (: 6.571, < 001), located in the frontal lobe (: 2.561, = 0.008), non-subventricular zone (SVZ) infringement (: 10.937, < 0.001), and methylated O-6-methylguanine-DNA methyltransferase (MGMT) promoter (mMGMTp) (: 9.737, < 0.001) were correlated with PsP, while GTR, non-SVZ infringement, and mMGMTp were further validated in multivariate analysis. Integrating quantitative MGMTp methylation levels from pyrosequencing, GTR, and non-SVZ infringement showed the best discriminative ability in the random forest model for derivation and validation set (AUC: 0.937, 0.911, respectively). Furthermore, a nomogram could effectively evaluate the importance of those markers in developing PsP (C-index: 0.916) and had a well-fitted calibration curve. Integrating those clinical, radiological, and molecular features provided a novel and robust method to distinguish PsP from TeP, which was crucial for subsequent clinical decision making, clinical trial enrollment, and prognostic assessment. By in-depth interrogation of perioperative markers, clinicians could distinguish PsP from TeP independent from advanced imaging.
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http://dx.doi.org/10.3389/fonc.2021.627325DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8093388PMC
April 2021

High Diversity of β-Glucosidase-Producing Bacteria and Their Genes Associated with Scleractinian Corals.

Int J Mol Sci 2021 Mar 29;22(7). Epub 2021 Mar 29.

Coral Reef Research Center of China, Guangxi Laboratory on the Study of Coral Reefs in the South China Sea, School of Marine Sciences, Guangxi University, Nanning 530004, China.

β-Glucosidase is a microbial cellulose multienzyme that plays an important role in the regulation of the entire cellulose hydrolysis process, which is the rate-limiting step in bacterial carbon cycling in marine environments. Despite its importance in coral reefs, the diversity of β-glucosidase-producing bacteria, their genes, and enzymatic characteristics are poorly understood. In this study, 87 β-glucosidase-producing cultivable bacteria were screened from 6 genera of corals. The isolates were assigned to 21 genera, distributed among three groups: Proteobacteria, Firmicutes, and Actinobacteria. In addition, metagenomics was used to explore the genetic diversity of bacterial β-glucosidase enzymes associated with scleractinian corals, which revealed that these enzymes mainly belong to the glycosidase hydrolase family 3 (GH3). Finally, a novel recombinant β-glucosidase, referred to as Mg9373, encompassing 670 amino acids and a molecular mass of 75.2 kDa, was classified as a member of the GH3 family and successfully expressed and characterized. Mg9373 exhibited excellent tolerance to ethanol, NaCl, and glucose. Collectively, these results suggest that the diversity of β-glucosidase-producing bacteria and genes associated with scleractinian corals is high and novel, indicating great potential for applications in the food industry and agriculture.
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http://dx.doi.org/10.3390/ijms22073523DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8037212PMC
March 2021

Interactions of fluoroquinolone antibiotics with sodium hypochlorite in bromide-containing synthetic water: Reaction kinetics and transformation pathways.

J Environ Sci (China) 2021 Apr 2;102:170-184. Epub 2020 Oct 2.

School of Marine Sciences, Guangxi Key Laboratory on the Study of Coral Reefs in the South China Sea, Guangxi University, Nanning 530004, China; Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai, China.

Seven popular fluoroquinolone antibiotics (FQs) in synthetic marine aquaculture water were subject to sodium hypochlorite (NaClO) disinfection scenario to investigate their reaction kinetics and transformation during chlorination. Reactivity of each FQ to NaClO was following the order of ofloxacin (OFL) > enrofloxacin (ENR) > lomefloxacin (LOM) > ciprofloxacin (CIP) ~ norfloxacin (NOR) >> pipemedic acid (PIP), while flumequine did not exhibit reactivity. The coexisting chlorine ions and sulfate ions in the water slightly facilitated the oxidation of FQs by NaClO, while humic acid was inhibitable to their degradation. The bromide ions promoted degradation of CIP and LOM, but restrained oxidation of OFL and ENR. By analysis of liquid chromatography with tandem mass spectrometry (LC-MS/MS), eight kinds of emerging brominated disinfection byproducts (Br-DBPs) caused by FQ were primarily identified in the chlorinated synthetic marine culture water. Through density functional theory calculation, the highest-occupied molecular orbital (HOMO) and the lowest-unoccupied molecular orbital (LUMO) characteristic as well as the charge distribution of the FQs were obtained to clarify transformation mechanisms. Their formation involved decarboxylation, ring-opening/closure, dealkylation and halogenation. Chlorine substitution occurred on the ortho-position of FQs's N4 and bromine substitution occurred on C8 position. The piperazine ring containing tertiary amine was comparatively stable, while this moiety with a secondary amine structure would break down during chlorination. Additionally, logK and logBAF of transformation products were calculated by EPI-Suite to analyze their bioaccumulation. The values indicated that Br-DBPs are easier to accumulate in the aquatic organism relative to their chloro-analogues and parent compounds.
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http://dx.doi.org/10.1016/j.jes.2020.09.013DOI Listing
April 2021

Genetic structure of Turbinaria peltata in the northern South China Sea suggest insufficient genetic adaptability of relatively high-latitude scleractinian corals to environment stress.

Sci Total Environ 2021 Jun 11;775:145775. Epub 2021 Feb 11.

Guangxi Laboratory on the Study of Coral Reefs in the South China Sea, Coral Reef Research Center of China, School of Marine Sciences, Guangxi University, Nanning 530004, China; Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai 519080, China. Electronic address:

With the rapid degradation of coral reefs due to global warming and anthropogenic impacts, relatively high-latitude areas, such as the northern South China Sea (SCS), are likely to become refuges for tropical coral species. Here we investigated the genetic features and adaptability of one dominant scleractinian coral species, Turbinaria peltata, in the northern SCS. A total of 81 samples from 5 sites were studied to explore potential mechanisms of adaptability to environmental stress as a result of climate change. Ten microsatellite markers developed in this study, one nuclear gene (internal transcribed spacer, ITS), and one mitochondrial gene (mitochondrial cytochrome oxidase subunit I gene, mtDNA COI) were used. Our results indicated that the genetic diversity of T. peltata in the northern SCS is low (A = 1.403-2.011, H = 0.105-0.248, H = 0.187-0.421) with the lowest in Dongfang population (DF) (A = 1.403, H = 0.22, H = 0.187). These results indicate that T. peltata has insufficient genetic adaptability and may unable to handle increasingly complex global changes. A significantly moderate genetic differentiation was observed among T. peltata populations (Φ = 0.167), in addition to a high genetic differentiation between DF and other populations (F = 0.272-0.536 > 0.25). The DF population near a fishing port was exposed to severe anthropogenic environmental stress, which may drive the extraordinarily high genetic differentiation between DF and other populations. Furthermore, the Mantel test results showed that the genetic differentiation of the other four populations was strongly correlated with the average sea surface temperature (SST) (R = 0.82, Mantel test P < 0.05) and geographical distance (R = 0.57, Mantel test P < 0.05). Our results suggest that the genetic structure of T. peltata in the relatively high-latitude of the SCS was significantly affected by average SST, geographical isolation, and anthropogenic activities. These findings provide a theoretical foundation for the protection of relatively high-latitude coral reefs.
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http://dx.doi.org/10.1016/j.scitotenv.2021.145775DOI Listing
June 2021

Distribution, fate and sources of polycyclic aromatic hydrocarbons (PAHs) in atmosphere and surface water of multiple coral reef regions from the South China Sea: A case study in spring-summer.

J Hazard Mater 2021 06 28;412:125214. Epub 2021 Jan 28.

School of Marine Sciences, SunYat-SenUniversity, Guangzhou 510006, China.

Our previous study revealed PAHs' wide occurrence in corals from multiple coral reef regions (CRRs) in the South China Sea. However, little is known about their occurrence, distribution, fate, and sources in the ambient environment of these CRRs. This study aimed to resolve these research gaps. The results showed ∑PAHs (total concentrations of 15 US EPA priority controlled PAHs exclude naphthalene) in the atmosphere (gas-phase: 0.31-49.6 ng m; particle-phase: 2.6-649 pg m) were mainly influenced by air mass origins. Southwesterly wind caused higher ∑PAHs than the southeasterly wind. The ∑PAHs in seawater from the nearshore (462 ± 244 ng L) was higher than that from offshore Zhongsha Islands (80.5 ± 72.1 ng L) because of the effect of terrigenous pollution and ocean current. Source apportionment indicated that the mixed sources of spilled oil and combustion from neighboring countries were the main contributors to PAHs in these CRRs. The total deposition fluxes showed that PAHs tended to migrate from the atmosphere to seawater. Global warming may inhibit this process, but PAHs still have a migration pattern of atmosphere-ocean-corals, which will further increase the environmental pressure on coral reef ecology.
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http://dx.doi.org/10.1016/j.jhazmat.2021.125214DOI Listing
June 2021

Nanopore long-read RNAseq reveals regulatory mechanisms of thermally variable reef environments promoting heat tolerance of scleractinian coral Pocillopora damicornis.

Environ Res 2021 04 24;195:110782. Epub 2021 Jan 24.

Guangxi Laboratory on the Study of Coral Reefs in the South China Sea, Coral Reef Research Center of China, School of Marine Sciences, Guangxi University, Nanning, China.

Some scleractinian corals exhibit high thermal adaptability to climate changes, although the mechanism of their adaptation is unclear. This study investigated the adaptability of scleractinian coral Pocillopora damicornis to thermally variable reef environments by applying a nanopore-based RNA sequencing method to characterize different transcription responses that promote heat tolerance of P. damicornis. We identified 1414 novel genes and optimized 6256 mis-annotated loci. Based on full-length transcriptome data, we identified complex alternative polyadenylation and alternative splicing events, which can improve our understanding of the genome annotation and gene structures of P. damicornis. Furthermore, we constructed differentially expressed lncRNA-mRNA co-expression networks, which may play a crucial role in the P. damicornis thermal adaptive response. KEGG function enrichment analysis revealed that P. damicornis from the high-temperature pool had a lower metabolic rate than that from the low-temperature pool. We hypothesize that metabolic readjustment, in the form of a lower metabolic rate, positively correlated with increased heat tolerance in P. damicornis in thermally variable reef environments. Our study provides novel insights into lncRNAs that promote thermally tolerance of scleractinian corals in the thermally variable reef environment, suggesting potential mechanisms for their adaptation to global warming in the future.
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http://dx.doi.org/10.1016/j.envres.2021.110782DOI Listing
April 2021

Synthesis, characterization and utilization of oxygen vacancy contained metal oxide semiconductors for energy and environmental catalysis.

Chemosphere 2021 Jun 4;272:129534. Epub 2021 Jan 4.

School of Marine Sciences, Guangxi University, Nanning, 530004, China; MOE Key Laboratory of New Processing Technology for Non-ferrous Metals and Materials, Guangxi Key Laboratory of Processing for Non-ferrous Metals and Featured Materials, Guangxi University, Nanning, 530004, China; Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai, 519080, China. Electronic address:

Developing novel functional materials with promising desired properties in enhancing energy conversion and lowering the catalytic reaction barriers is essential for the demand to solve the increasingly severe energy and environmental crisis nowadays. Metal oxide semiconductors (MOS) are widely used in the field of catalysis because of its excellent catalytic characteristics. Introduction of defects, in addition to the adjustment of composition and atomic arrangement in the materials can effectively improve the materials' catalytic performance. Especially, introducing oxygen vacancies (OVs) into the lattice structure of MOS has been developed as a facile route to improve MOS's optical and electronic transmission characteristics. And a large number of metal oxides with rich OVs have been served in oxygen reduction reaction (ORR), oxygen evolution reaction (OER), hydrogen evolution reaction (HER), carbon dioxide reduction reaction (CO-RR) photo-degradation of organic pollutants, etc. This small review briefly outlines some preparation techniques to introduce OVs into MOS, and the characterization techniques to identify and quantify the OVs in MOS. The applications of OVs contained MOS especially in energy and environmental catalysis areas are also discussed. The effects of OVs types and concentrations on the catalytic performances are deliberated. Finally, the defective structure-catalytic property relationship is highlighted, and the future status and opportunities of MOS containing OVs in the catalytic field are suggested.
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http://dx.doi.org/10.1016/j.chemosphere.2021.129534DOI Listing
June 2021

Intergeneric and geomorphological variations in Symbiodiniaceae densities of reef-building corals in an isolated atoll, central South China Sea.

Mar Pollut Bull 2021 Feb 25;163:111946. Epub 2020 Dec 25.

South China Institute of Environmental Sciences, the Ministry of Ecology and Environment of PRC, Guangzhou 510530, China.

The healthy status of corals in the isolated atolls of the central South China Sea (SCS) remains unclear. Symbiodiniaceae density (SD) can effectively reflect the thermal tolerance and health of hard corals. Here, the SDs of 238 samples from the Huangyan Atoll (HA) were analyzed. The results revealed significantly intergeneric and geomorphological differences in SD. Intergeneric variation may reflect that corals with high SD have stronger thermal tolerance. Geomorphic analysis showed that the SDs at the outer reef slope were higher than in the lagoon. Hydrodynamics and sea surface temperature were likely the main influencing factors. Most notably, corals in SCS HA had higher SDs than those at neighboring reefs, indicating that their thermal tolerance were strong, which may be related to HA's local upwelling. These results suggest that the HA has the potential to serve as a refuge for corals, but increasing human disturbance limit its function.
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http://dx.doi.org/10.1016/j.marpolbul.2020.111946DOI Listing
February 2021

Classification of Progression Patterns in Glioblastoma: Analysis of Predictive Factors and Clinical Implications.

Front Oncol 2020 3;10:590648. Epub 2020 Nov 3.

Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.

Background: This study was designed to explore the progression patterns of IDH-wildtype glioblastoma (GBM) at first recurrence after chemoradiotherapy.

Methods: Records from 247 patients who underwent progression after diagnosis of IDH-wildtype GBM was retrospectively reviewed. Progression patterns were classified as either local, distant, subependymal or leptomeningeal dissemination based on the preoperative and serial postoperative radiographic images. The clinical and molecular characteristics of different progression patterns were analyzed.

Results: A total of 186 (75.3%) patients had local progression, 15 (6.1%) patients had distant progression, 33 (13.3%) patients had subependymal dissemination, and 13 (5.3%) patients had leptomeningeal dissemination. The most favorable survival occurred in patients with local progression, while no significant difference of survival was found among patients with distant progression, subependymal or leptomeningeal dissemination who were thereby reclassified into non-local group. Multivariable analysis showed that chemotherapy was a protective factor for non-local progression, while gender of male, subventricular zone (SVZ) involvement and O-methylguanine-DNA-methyltransferase (MGMT) promoter methylation were confirmed as risk factors for non-local progression ( < 0.05). Based on the factors screened by multivariable analysis, a nomogram was constructed which conferred high accuracy in predicting non-local progression. Patients in non-local group could be divided into long- and short-term survivors who differed in the rates of SVZ involvement, MGMT promoter methylation and reirradiation ( < 0.05), and a nomogram integrating these factors showed high accuracy in predicting long-term survivors.

Conclusion: Patients harboring different progression patterns conferred distinct clinical and molecular characteristics. Our nomograms could provide theoretical references for physicians to make more personalized and precise treatment decisions.
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http://dx.doi.org/10.3389/fonc.2020.590648DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7673412PMC
November 2020

Microbiome community and complexity indicate environmental gradient acclimatisation and potential microbial interaction of endemic coral holobionts in the South China Sea.

Sci Total Environ 2021 Apr 3;765:142690. Epub 2020 Oct 3.

Guangxi Laboratory on the Study of Coral Reefs in the South China Sea, Guangxi University, Nanning, China; Coral Reef Research Center of China, Guangxi University, Nanning, China; School of Marine Sciences, Guangxi University, Nanning, China.

Regional acclimatisation and microbial interactions significantly influence the resilience of reef-building corals facing anthropogenic climate change, allowing them to adapt to environmental stresses. However, the connections between community structure and microbial interactions of the endemic coral microbiome and holobiont acclimatisation remain unclear. Herein, we used generation sequencing of internal transcribed spacer (ITS2) and 16S rRNA genes to investigate the microbiome composition (Symbiodiniaceae and bacteria) and associated potential interactions of endemic dominant coral holobionts (Pocillopora verrucosa and Turbinaria peltata) in the South China Sea (SCS). We found that shifts in Symbiodiniaceae and bacterial communities of P. verrucosa were associated with latitudinal gradient and climate zone changes, respectively. The C1 sub-clade consistently dominated the Symbiodiniaceae community in T. peltata; yet, the bacterial community structure was spatially heterogeneous. The relative abundance of the core microbiome among P. verrucosa holobionts was reduced in the biogeographical transition zone, while bacterial taxa associated with anthropogenic activity (Escherichia coli and Sphingomonas) were identified in the core microbiomes. Symbiodiniaceae and bacteria potentially interact in microbial co-occurrence networks. Further, increased bacterial, and Symbiodiniaceae α-diversity was associated with increased and decreased network complexity, respectively. Hence, Symbiodiniaceae and bacteria demonstrated different flexibility in latitudinal or climatic environmental regimes, which correlated with holobiont acclimatisation. Core microbiome analysis has indicated that the function of core bacterial microbiota might have changed in distinct environmental regimes, implying potential human activity in the coral habitats. Increased bacterial α diversity may lead to a decline in the stability of coral-microorganism symbioses, whereas rare Symbiodiniaceae may help to retain symbioses. Cladocopium, γ-proteobacteria, while α-proteobacteria may have been the primary drivers in the Symbiodiniaceae-bacterial interactions (SBIs). Our study highlights the association between microbiome shift in distinct environmental regimes and holobiont acclimatisation, while providing insights into the impact of SBIs on holobiont health and acclimatisation during climate change.
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http://dx.doi.org/10.1016/j.scitotenv.2020.142690DOI Listing
April 2021

sp. nov., a bacterium isolated from coral , and proposal of fam. nov. to accommodate and .

Int J Syst Evol Microbiol 2020 Nov;70(11):5880-5887

School of Marine Sciences, Guangxi University, Nanning 530004, PR China.

A novel Gram-stain-negative, non-endospore-forming, motile, and aerobic bacterial strain, M105, was isolated from coral , and was subjected to a polyphasic taxonomic study. Global alignment based on 16S rRNA gene sequences indicated that M105 shares the highest sequence identity of 94.5 % with GYP-15. The average nucleotide identity (ANI) and average amino acid identity (AAI) between M105 and GYP-15 was 69.8 and 71.6 %, respectively. On the basis of the results of phenotypic, chemotaxonomic, phylogenetic, phylogenomic, and comparative genomic analyses, it is concluded that M105 should represent a novel species in the genus , for which the name sp. nov. is proposed. The type strain is M105 (=MCCC 1K03773= KCTC 72442). Furthermore, the family was classified into two families on the basis of phylogenetic, phylogenomic, polar lipid profile and motility variations. The novel family fam. nov. is proposed to accommodate the genera and .
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http://dx.doi.org/10.1099/ijsem.0.004489DOI Listing
November 2020

Spatial distribution, source identification, and risk assessment of organochlorines in wild tilapia from Guangxi, South China.

Sci Rep 2020 09 16;10(1):15179. Epub 2020 Sep 16.

School of Marine Sciences, Guangxi University, Nanning, 530004, China.

Seventy-five wild tilapia samples from six rivers (ten sites) in Guangxi province were collected and analyzed for 53 organochlorine compounds. DDTs, endosulfan, and PCBs were the most dominant compounds found in this study. Tiandong County (TD) and Guigang City (GG) sites were found to be heavily contaminated with high levels of endosulfan (385-925 ng/g lw) and/or DDTs (20.1-422 ng/g lw). The diagnostic ratios indicated that the residues of DDTs and endosulfan in wild tilapia are associated with historical applications as well as the recent introduction of technical DDTs and endosulfan at some sampling sites. The correlation between total length, body mass, and organochlorines (OCs) was higher than the correlation between age and lipid content. There was no significant correlation between organochlorine pesticides (OCPs) and lipid content. Therefore, for organisms, the feeding intensity (related to length and mass) of fish could better reflect degree of pollution than exposure time (age) of fish. The hazardous ratios for the 50th and 95th percentile data of OCPs and PCBs in fish were both below 1, suggesting that daily exposure to OCPs and PCBs yields a lifetime cancer risk lower than 1 in 10,000.
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http://dx.doi.org/10.1038/s41598-020-72160-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7495417PMC
September 2020

Influences of phosphorus concentration and porewater advection on phosphorus dynamics in carbonate sands around the Weizhou Island, northern South China Sea.

Mar Pollut Bull 2020 Nov 11;160:111668. Epub 2020 Sep 11.

Guangxi Laboratory on the Study of Coral Reefs in the South China Sea, Coral Reef Research Centre of China, School of Marine Sciences, Guangxi University, Nanning 530004, PR China.

A series of flow-through reactor experiments were undertaken to assess the potential effect of porewater advection and dissolved inorganic phosphorus (DIP) concentration on benthic DIP dynamics in permeable sediments collected from the Weizhou Island, northern South China Sea. The flux of DIP ranged from -0.13 to 0.05 mmol m h, and the reversal from DIP efflux to influx occurred when the DIP concentration reached a threshold. DIP release from the sediment into the seawater peaked at intermediate advection rate, which perhaps provide optimum conditions for DIP release related to CaCO dissolution. Phosphorus limitation in seawater could be relieved by DIP release from the sediment, and CaCO-bound P in carbonate sands may play a major role in benthic DIP release and decrease in the molar nitrogen/phosphorus ratio in seawater around the Weizhou Island.
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http://dx.doi.org/10.1016/j.marpolbul.2020.111668DOI Listing
November 2020

gen. nov., sp. nov., a member of the family isolated from coral .

Int J Syst Evol Microbiol 2020 Nov;70(11):5620-5626

School of Marine Sciences, Guangxi University, Nanning 530004, PR China.

A novel Gram-stain-negative, non-endospore-forming, non-motile, aerobic bacterium (strain R33) was isolated from coral and subjected to a polyphasic taxonomic study. The G+C content was 44.5 mol%. The only detected respiratory quinone was menaquinone 6 (MK-6). The major cellular fatty acids were iso-C and iso-C ω6. The major polar lipids were phosphatidylethanolamine and two unidentified lipids. Global alignment based on 16S rRNA gene sequences indicated that strain R33 shares the highest sequence identity of 93.2 % with A6B8 in the family . Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain R33 forms a distinct branch in a stable clade comprising strain R33 and members of the genera , , and . The phylogenomic analysis also supported this 16S rRNA gene-based phylogenetic result. Comparative genomic analysis indicated that strain R33 is rich in AraC-type DNA-binding domain-containing protein-coding genes, which means the regulation of carbon utilization is very complex. Low 16S rRNA gene identity, different polar lipids and/or cellular fatty acid profiles could readily distinguish strain R33 from any validly published type strains. Therefore, strain R33 is suggested to represent a new species in a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain is R33 (=MCCC 1K03853=KCTC 72443).
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http://dx.doi.org/10.1099/ijsem.0.004452DOI Listing
November 2020

Occurrence, source, and the fate of antibiotics in mariculture ponds near the Maowei Sea, South China: Storm caused the increase of antibiotics usage.

Sci Total Environ 2021 Jan 21;752:141882. Epub 2020 Aug 21.

Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai 519080, China; School of Marine Sciences, Sun Yat-Sen University, Guangzhou 510006, China.

Antibiotic residues in mariculture environments have been detected globally, while little information is available about their dynamic levels, source, behavior, and fate during the whole culture process. In this study, the dynamic occurrence, bioaccumulation, source, fate, and human dietary risk of 19 antibiotics were investigated in different breeding stages of mariculture ponds near the Maowei Sea, South China. Fourteen antibiotics, including three sulfonamides (SAs), five fluoroquinolones (FQs), three macrolides (MLs), and two chloramphenicols (CAPs), were detected in the mariculture ponds, with FQs being the most abundant antibiotics. Significant variations of antibiotic concentration occurred during the whole culture process. Severe weather, especially typhoons and rainstorms, resulted in the average highest levels of ∑antibiotics (mean: 567 ng L) in mariculture ponds. The source apportionment estimated for the mariculture ponds showed that direct application was the primary source of antibiotics (91.2%). The antibiotics in mariculture ponds were mainly discharged through aquaculture wastewater (65.8%) and settling particles (33.8%). The estimated annual input of antibiotics into the Maowei Sea was 2.24 times higher through the two main rivers (48.0 kg a) than through the mariculture wastewater (24.1 kg a). The apparent bioaccumulation factors (ABAFs) confirmed that young and adult tilapia accumulated more sulfamethoxazole (SMX) and norfloxacin (NOX), respectively. The result from the estimated daily intakes suggested that the antibiotics in the seafood could not pose a risk to human health by dietary exposure assessment. CAPSULE: Big variation of antibiotic concentration occurred during the whole culture process in the mariculture farms, and the storm increased antibiotic application.
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http://dx.doi.org/10.1016/j.scitotenv.2020.141882DOI Listing
January 2021

Simultaneous Determination of Urine Methotrexate, 7-Hydroxy Methotrexate, Deoxyaminopteroic Acid, and 7-Hydroxy Deoxyaminopteroic Acid by UHPLC-MS/MS in Patients Receiving High-dose Methotrexate Therapy.

Anal Sci 2020 Dec 14;36(12):1479-1486. Epub 2020 Aug 14.

Department of Pharmacy, Beijing Tiantan Hospital, Capital Medical University.

Nephrotoxicity, the most important toxicity in high-dose methotrexate (MTX) therapy, is partly caused by the formation of crystal deposits in the kidney due to poor water solubility of MTX and its metabolites 7-hydroxy methotrexate (7-OH MTX), deoxyaminopteroic acid (DAMPA) and 7-hydroxy deoxyaminopteroic acid (7-OH DAMPA). Plasma MTX level-guided urine alkalinization, leucovorin rescue and glucarpidase detoxification are common strategies to overcome MTX-related nephrotoxicity. However, overestimation is a problem for MTX analysis by immunoassays due to the cross-reactivity of MTX metabolites (7-OH MTX and DAMPA). An UHPLC-MS/MS method for the simultaneous determination of MTX, 7-OH MTX, DAMPA and 7-OH DAMPA in human urine was developed, validated and applied in clinical practice. Samples were treated by one-step protein precipitation and analyzed within 3 min. The calibration range was 0.02 to 4 μmol/L for MTX and DAMPA, and 0.1 to 20 μmol/L for 7-OH MTX and 7-OH DAMPA. For all analytes, the intra-day and inter-day bias and imprecision were -8.0 to 7.6 and <9.0%, the internal standard normalized recovery and matrix factor were 92.34 to 109.49 and <20.68%. The plasma MTX and 7-OH MTX levels increased with the urine drug levels, age, serum creatinine and alanine transaminase, but urine could not replace blood for MTX monitoring due to their poor correlation (R, 0.16 to 0.51). Dose-normalized urine and plasma MTX and 7-OH MTX levels were similar between different patient groups (urine pH <7 or ≥7). Due to the large inter-individual variance of the analytes levels in both plasma and urine, these findings should be treated with caution.
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http://dx.doi.org/10.2116/analsci.19P481DOI Listing
December 2020

An approach for assessing ecosystem-based adaptation in coral reefs at relatively high latitudes to climate change and human pressure.

Environ Monit Assess 2020 Aug 11;192(9):579. Epub 2020 Aug 11.

Guangxi Laboratory on the Study of Coral Reefs in the South China Sea, Guangxi University, Nanning, 530004, Guangxi, China.

Relatively high-latitude waters are supposed as a refuge for corals under ocean warming. A systematic assessment of the Weizhou Island reef in the northern South China Sea, a relatively high-latitude region, shows that the ecosystem restoration index decreased from 0.96 to 0.62 during the period between 1990 and 2015. Although the biotic community, supporting services, and regulating services remained at good or very good states, the provisioning services, cultural services, and especially habitat structure deteriorated to very poor or moderate states. Gray relational analysis showed that these ecological declines exhibited a strong relationship with human pressures from tourism activities and the petrochemical industry. The recoveries of the biotic community and supporting services that benefited from wintertime warming appeared to be partly offset by intensive human pressures. The long-term effects on ecosystem structure and functions suggest that anthropogenic disturbances have impaired the possibility of this area serving as a potential thermal refuge for reef-building corals in the South China Sea. This study thus provides an integrated approach for assessing the adaptive responses of coral reef ecosystems to climate change and local human activities.
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http://dx.doi.org/10.1007/s10661-020-08534-5DOI Listing
August 2020

Diazotroph Diversity Associated With Scleractinian Corals and Its Relationships With Environmental Variables in the South China Sea.

Front Physiol 2020 17;11:615. Epub 2020 Jun 17.

State Key Laboratory of Tropical Oceanography (LTO), South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, China.

Coral reef ecosystems cannot operate normally without an effective nitrogen cycle. For oligotrophic coral reef areas, coral-associated diazotrophs are indispensable participants in the nitrogen cycle. However, the distribution of these diazotrophs and the correlation with the physical and chemical variables of the surrounding seawater remain unclear. To this end, 68 scleractinian coral colonies were sampled from 6 coral reef areas with different environmental variables in the South China Sea to investigate the composition of associated diazotrophs based on gene amplification using high-throughput sequencing. The six coral reefs can be clearly divided into two types (fringing reefs and island reefs), are affected by varying degrees of human activities and are located at different latitudes from 9°20'06"N to 22°34'55"N with different seawater temperatures. Alpha- and beta-diversity analyses showed that the distribution of diazotrophs among coral reefs exhibited significant geographical fluctuations ( ≤ 0.05) and non-significant interspecific fluctuations ( > 0.05). The predominant bacterial phyla included Proteobacteria, Chlorobi, Cyanobacteria, and two unclassified phyla. Chlorobi exhibited a relative abundance of 47-96% in coral samples from the high-latitude Daya Bay fringing reef affected by eutrophication. Unclassified bacteria II, with a relative abundance of 28-87%, was found in all coral samples from the midlatitude Luhuitou fringing reef affected by eutrophication. However, unclassified bacteria I and Proteobacteria dominated (>80% relative abundance) in most of the coral samples from the Weizhou Island fringing reef, which is far from land, and three island reefs (Huangyan Island, Xinyi Reef, and Sanjiao Reef) at relatively low latitudes. At the genus level, some core diazotrophs were found in different coral sample groups. In addition, correlation analysis with various environmental variables revealed that the variables were positively or negatively correlated with different diazotrophic genera. Coral-associated diazotrophs were common among coral individuals. However, their composition was closely related to the different environmental variables. These results provide insights into the geographical distribution characteristics of coral-associated diazotrophs and their evolutionary trends in response to environmental change in the South China Sea.
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http://dx.doi.org/10.3389/fphys.2020.00615DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7314963PMC
June 2020

Bioaccumulation and trophic transfer of organophosphate esters in tropical marine food web, South China Sea.

Environ Int 2020 10 2;143:105919. Epub 2020 Jul 2.

State Key Laboratory of Organic Geochemistry and Guangdong Key Laboratory of Environmental Resources Utilization and Protection, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China.

Environmental exposure to organophosphate esters (OPEs) continues to be a concern. Little is known about their bioaccumulation and trophodynamics, especially in tropical food webs. This study collected seawater and fifteen types of organism from a tropical ecosystem, South China Sea, to investigate the species-specific compositional, bioaccumulation, and trophic transfer of OPEs. The total concentrations of 11 target OPEs (ng/g dw) in the organisms decreased with the increase of their trophic levels in the order: phytoplankton (922) > zooplankton (660) > oysters (309) > crabs (225) > coral tissues (202) > fishes (58.2). The composition profiles (relative abundances) of OPEs were different among the species of organisms, which is likely affected by metabolism and the physicochemical property of OPEs. The trophic biomagnification of tripentyl phosphate (TPTP) in the pelagic food web was unexpected and requires further investigation. The trophic magnification factors (TMFs) of OPEs were generally lower in this tropical aquatic food web than in temperate and frigid aquatic food web. Our analysis suggests that there is a significant positive linear correlation between latitude and TMF. Intakes of OPEs through the consumption of the seafood involved in this work does not pose health risk to adults.
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http://dx.doi.org/10.1016/j.envint.2020.105919DOI Listing
October 2020

Dynamic distribution of microplastics in mangrove sediments in Beibu Gulf, South China: Implications of tidal current velocity and tidal range.

J Hazard Mater 2020 11 19;399:122849. Epub 2020 May 19.

School of Marine Sciences, Guangxi University, Nanning 530004, PR China; Guangxi Laboratory on the Study of Coral Reefs in the South China Sea, Guangxi University, Nanning 530004, PR China; Coral Reef Research Center of China, Guangxi University, Nanning 530004, PR China. Electronic address:

Hydrodynamic factors have always been considered as the predominant factors determining the transportation of suspended particulate matter in tidal flats. However, whether such factors also affect the transportation of microplastics (MPs) in mangrove forests remains largely unknown. In our study, the extent to which the two most critical hydrodynamic factors (tidal range and current velocity) impact the distribution of MPs in mangrove sediments was evaluated. In the different zones of the mangrove forest, strong linear relationships were observed between the tidal current velocity and MPs content during both the flood and ebb tide periods (p = 0.002, R > 0.837). Similarly, in the same mangrove forest, the MPs content significantly differed, and a general increasing trend was found from the seaward boundary (ranging from 80 ± 16 item/kg to 1020 ± 89 item/kg) to the landward boundary (ranging from 520 ± 32 to 6040 ± 114 item/kg). The MPs growth rates showed obvious linear relationships with the tidal current velocity (p < 0.005, R > 0.905), but there were no relationships with the tidal range (p = 0.717). The results of this work highlight that tidal current velocity and tidal range should be taken into account when exploring the mechanisms of MPs distributions in mangrove ecosystems.
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http://dx.doi.org/10.1016/j.jhazmat.2020.122849DOI Listing
November 2020

Potential molecular traits underlying environmental tolerance of Pavona decussata and Acropora pruinosa in Weizhou Island, northern South China Sea.

Mar Pollut Bull 2020 Jul 11;156:111199. Epub 2020 May 11.

Guangxi Laboratory on the Study of Coral Reefs in the South China Sea, Guangxi University, Nanning 530004, China; Coral Reef Research Center of China, Guangxi University, Nanning 530004, China; School of Marine Sciences, Guangxi University, Nanning 530004, China.

Coral species display varying susceptibilities to biotic or abiotic stress. To address the causes underlying this phenomenon, we profiled the Symbiodiniaceae clade type, bacterial communities and coral transcriptome responses in Pavona decussata and Acropora pruinosa, two species displaying different environmental tolerances in the Weizhou Island. We found that C1 was the most dominant Symbiodiniaceae subclade, with no difference detected between A. pruinosa and P. decussata. Nevertheless, P. decussata exhibited higher microbial diversity and significantly different community structure compared with that of A. pruinosa. Transcriptome analysis revealed that coral genes with significantly high expression in P. decussata were mostly related to immune and stress-resistance responses, whereas, those with significantly low expression were metabolism-related. We postulate that the higher tolerance of P. decussata as compared with that of A. pruinosa is the result of several traits, such as higher microbial diversity, different dominant bacteria, higher immune and stress-resistant response, and lower metabolic rate.
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http://dx.doi.org/10.1016/j.marpolbul.2020.111199DOI Listing
July 2020

Facile Design and Hydrothermal Synthesis of InO Nanocube Polycrystals with Superior Triethylamine Sensing Properties.

ACS Omega 2020 May 12;5(20):11466-11472. Epub 2020 May 12.

School of Marine Sciences, Guangxi University, Nanning 530004, China.

Triethylamine (TEA), a typical kind of volatile organic compound, is widely used as an industrial solvent, which is a threat to environment and human health. In this paper, a novel InO nanocube gas sensing material with high sensing performances was synthesized through a simple one-pot hydrothermal method. The gas sensing test results are satisfactory such that the single phase of InO nanocubes even exhibits a quite higher response of 12-10 ppm of TEA at a lower temperature (180 °C) than many conventional InO-based complexes and maintains high consistency in morphology and stability after the consecutive tests of 2 months. This work provides a facile and quite effective gas sensing material for TEA gas monitoring with high sensitivity and stability and good selectivity at lower working temperature that can be prepared in batch and further used as templates.
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http://dx.doi.org/10.1021/acsomega.0c00497DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7254500PMC
May 2020

Thermal acclimation increases heat tolerance of the scleractinian coral Acropora pruinosa.

Sci Total Environ 2020 Sep 11;733:139319. Epub 2020 May 11.

Guangxi Laboratory on the Study of Coral Reefs in the South China Sea, Guangxi University, Nanning, China; Coral Reef Research Center of China, Guangxi University, Nanning, China; School of Marine Sciences, Guangxi University, Nanning, China.

Field ecological observations indicate that scleractinian coral exposed to early thermal stress are likely to develop higher tolerance to subsequent heat stress. The causes of this phenomenon, however, remain enigmatic. To unravel the mechanisms underlying the increased heat tolerance, we applied different thermal treatments to the scleractinian coral Acropora pruinosa and studied the resulting differences in appearance, physiological index, Symbiodiniaceae and bacterial communities, and transcriptome response. We found that early heat stress improved the thermal tolerance of the coral holobiont. After thermal acclimation, the community structure and symbiotic bacterial diversity in the microbiota were reorganized, whereas those of Symbiodiniaceae remained stable. RNA-seq analysis revealed that the downregulated coral host genes were mainly involved in pathways relating to metabolism, particularly the nitrogen metabolism pathway. This indicates that thermal acclimation led to decrease in the metabolism level in the coral host, which might be a self-protection mechanism. We suggest that thermal acclimation may increase scleractinian coral thermal tolerance by slowing host metabolism, altering the dominant bacterial population, and increasing bacterial diversity. This study offers new insights into the adaptive potential of scleractinian coral to heat stress from global warming.
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http://dx.doi.org/10.1016/j.scitotenv.2020.139319DOI Listing
September 2020
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