Publications by authors named "Ke-Jing Huang"

72 Publications

High-power-energy proton supercapacitor based on interface-adapted durable polyaniline and hexagonal tungsten oxide.

J Colloid Interface Sci 2021 May 29;601:727-733. Epub 2021 May 29.

College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China. Electronic address:

Supercapacitors are high power energy storage devices, however, their application are remain limited by the low energy density. Developing high capacity electrode materials and constructing devices with high operating voltage are effective ways to solve this problem. Herein, performance of polyaniline (PANI) electrode materials is dramatically enhanced by engineering robust PANI/carbon interfaces, through assembling PANI nanorod array on rose petals derived carbon network (RPDCN). The structure of the PANI is optimized by adjusting the concentration of the aniline precursor. The unique structure enables the prepared PANI/RPDCN composite show a high capacitance of 636 F g at 0.5 A g, based on the total weight of PANI and RPDCN substrate. The robust interface effectively prolonged the composite electrode stably cycled for over 2000 cycles at 2 A g with a capacity retention of 89%. When coupled with a hexagonal tungsten oxide (h-WO) anode, a high-power asymmetric proton supercapacitor with high energy densities (29.0 Wh kg/0.61 kW kg and 21.4 Wh kg/19.51 kW kg) was assembled. This work provides an effective and eco-friendly route toward superior PANI electrodes and proposes a promising high-power energy storage system using proton as working ion.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jcis.2021.05.157DOI Listing
May 2021

Engineering stable and fast sodium diffusion route by constructing hierarchical MoS hollow spheres.

J Colloid Interface Sci 2021 Aug 24;595:43-50. Epub 2021 Mar 24.

College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China. Electronic address:

Two-dimensional layered transition metal dichalcogenides, such as MoS, have been considered to be a promising anode material for sodium storage. However, their performance have been limited by the sluggish sodium diffusion kinetics. In this work, high performance anode material was obtained through constructing hierarchical MoS nanosheets assembled hollow spheres. The used self-templating method show more feasibility than the commonly reported template removal-involved routes. The prepared hollow structure can also provide rapid and stable electron/sodium ion transport without the assistance of conducting substrates, which enables the MoS anodes exhibit a high specific capacity of 527 mAh g at 0.1 A g. Even at a high current density of 1 A g, capacity of 357 mAh g can still be obtained after 500 cycles (capacity retention ~94.5%). This work provides a facile way towards high performance MoS anode materials for sodium-ion battery.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jcis.2021.03.112DOI Listing
August 2021

Stimulating the Reversibility of Sb S Anode for High-Performance Potassium-Ion Batteries.

Small 2021 Mar 15;17(10):e2008133. Epub 2021 Feb 15.

Key Laboratory of Advanced Energy Materials Chemistry (Ministry of Education), College of Chemistry, Nankai University, Tianjin, 300071, China.

Conversion-alloy sulfide materials for potassium-ion batteries (KIBs) have attracted considerable attention because of their high capacities and suitable working potentials. However, the sluggish kinetics and sulfur loss result in their rapid capacity degeneration as well as inferior rate capability. Herein, a strategy that uses the confinement and catalyzed effect of Nb O layers to restrict the sulfur species and facilitate them to form sulfides reversibly is proposed. Taking Sb S anode as an example, Sb S and Nb O are dispersed in the core and shell layers of carbon nanofibers (C NFs), respectively, constructing [email protected] structure Sb S [email protected] O -C NFs. Benefiting from the bi-functional Nb O layers, the electrochemical reversibility of Sb S is stimulated. As a result, the Sb S [email protected] O -C NFs electrode delivers the rapidest K-ion diffusion coefficient, longest cycling stability, and most excellent rate capability among the controlled electrodes (347.5 mAh g is kept at 0.1 A g after 100 cycles, and a negligible capacity degradation (0.03% per cycle) at 2.0 A g for 2200 cycles is delivered). The enhanced K-ion storage properties are also found in SnS [email protected] O -C NFs electrode. Encouraged by the stimulated reversibility of Sb S and SnS anodes, other sulfides with high electrochemical performance also could be developed for KIBs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/smll.202008133DOI Listing
March 2021

1T-Phase MoS with large layer spacing supported on carbon cloth for high-performance Na storage.

J Colloid Interface Sci 2021 Feb 23;583:579-585. Epub 2020 Sep 23.

College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China. Electronic address:

The design and construction of advanced electrode materials is important to the development of high-performance electrochemical energy storage devices. In this paper, V-doped 1T MoS nanosheets with a large layer spacing are grown on carbon cloth (CC) via a one-step hydrothermal method. The resulting material features abundant edge sites and active centers, and its large layer spacing facilitates the interlayer shuttle of Na. Doping with V buffers the volume change and maintains the integrity of MoS. The layered structure of the composite featuring CC as a conductive substrate effectively prevents the agglomeration of MoS during the electrochemical process. When used as an anode material for a Na battery, the material displays a high first-cycle irreversible discharge specific capacity of 1234.9 mAh g. A specific capacity of 453.2 mAh g is obtained after 100 cycles at a current density of 200 mA g. This work provides an effective and eco-friendly route toward obtaining superior MoS electrodes for high-performance Na storage.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jcis.2020.09.055DOI Listing
February 2021

Stabilizing Metallic Iron Nanoparticles by Conformal Graphitic Carbon Coating for High-Rate Anode in Ni-Fe Batteries.

Nano Lett 2020 Mar 11;20(3):1700-1706. Epub 2020 Feb 11.

Department of NanoEngineering, University of California San Diego, La Jolla, California 92093, United States.

Nickel-iron (Ni-Fe) batteries are promising candidates for large-scale energy storage due to their high safety and low cost. However, their power density and cycling efficiency remain limited by the poor kinetics of the Fe anode. Herein, we report high-performance Fe anodes based on active Fe nanoparticles conformally coated with carbon shells, which were synthesized from low-cost precursors using a scalable process. Such core-shell structured C-Fe anodes offer high electrochemical activity and stability. Specifically, a high specific capacity of 208 mAh g at a current density of 1 A g (based on the total weight of Fe and C) and a capacity retention of 93% after 2000 cycles at 4 A g can be achieved. When coupled with a Ni cathode, such a full cell battery can deliver a high energy density of 101.0 Wh kg at power density of 0.81 kW kg and 51.6 Wh kg at 8.2 kW kg (based on the mass of the electrode materials), among the best energy and power performance among Ni-Fe batteries reported results. Thus, this work may provide an effective and scalable route toward high-performance anodes for high-power and long-life Ni-Fe batteries.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.nanolett.9b04867DOI Listing
March 2020

A high-energy sandwich-type self-powered biosensor based on DNA bioconjugates and a nitrogen doped ultra-thin carbon shell.

J Mater Chem B 2020 02;8(7):1389-1395

College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China.

A high-energy self-powered sensing platform for the ultrasensitive detection of proteins is developed based on enzymatic biofuel cells (EBFCs) by using DNA bioconjugate assisted signal amplification. A nitrogen doped ultra-thin carbon shell/gold nanoparticle (N-UHCS/AuNPs) composite was prepared and applied as an electrode supporting substrate to improve the enzyme load. The biocathode of the self-powered sensor is constructed through the step-by-step modification of N-UHCS/AuNPs and bilirubin oxidase (BOD) on carbon paper (CP). To fabricate the bioanode, SiO2 [email protected] ([email protected]) bioconjugates were prepared and modified on CP. When there is a target protein, the aptamer recognizes it and causes the [email protected] bioconjugate to fall off the bioanode, resulting in a significant increase in the open circuit voltage (EOCV) of the sensing device. Under optimal conditions, the developed biosensor shows a wide linear range of 0.1-2000 ng mL-1 with a low detection limit of 21.5 pg mL-1 (S/N = 3). This work shows an effective assay for the sensitive detection of biomolecules by coupling EBFCs, DNA bioconjugates and the biosensing characteristics of smart nanostructures.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/c9tb02574jDOI Listing
February 2020

A highly sensitive electrochemical biosensor for protein based on a tetrahedral DNA probe, N- and P-co-doped graphene, and rolling circle amplification.

Anal Bioanal Chem 2020 Feb 3;412(4):915-922. Epub 2020 Jan 3.

College of Chemistry and Chemical Engineering, Xinyang Normal University, 237# Chang An Road, Xinyang, 464000, Henan, China.

A tetrahedral DNA probe can effectively overcome the steric effects of a single-stranded probe to obtain well-controlled density and minimize nonspecific adsorption. Herein, a highly sensitive electrochemical biosensor is fabricated for determination of protein using a tetrahedral DNA probe and rolling circle amplification (RCA). N- and P-co-doped graphene (NP-rGO) is prepared, and AuNPs are then electrodeposited on it for DNA probe immobilization. Benefitting from the synergistic effects of the excellent electrical conductivity of NP-rGO, the stability of the tetrahedral DNA probe and the signal amplification of RCA, the biosensor achieves a low limit of 3.53 × 10 M for thrombin and a wide linear range from 1 × 10 to 1 × 10 M. This study provides a sensitive and effective method for the detection of protein in peripheral biofluids, and paves the way for future clinical diagnostics and treatment of disease. Graphical abstract.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00216-019-02314-yDOI Listing
February 2020

Recent advances in nanomaterial-based electrochemical and optical sensing platforms for microRNA assays.

Analyst 2019 May 27;144(9):2849-2866. Epub 2019 Mar 27.

College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China.

MicroRNA (MiRNA) plays a crucial role in biological cells to enable assessment of a cancer's development stage. Increasing evidence has shown that the accurate and sensitive detection of miRNA holds the key toward correct disease diagnosis. However, some characteristics of miRNAs, such as their short chains, low concentration, and similar sequences, make it difficult to detect miRNA in biological samples. Nanomaterials usually have good optical, electronic, and mechanical properties and therefore provide new possibilities for improving the performance of miRNA assays. Many different sorts of nanomaterials, including metal nanomaterials, carbon nanomaterials, quantum dots, and transition-metal dichalcogenides, have been used to construct optical and electrochemical assays for miRNA and have shown attractive results. This review describes recent efforts in the application of nanomaterials as sensing elements in electrochemical and optical miRNA assays. The analytical figures of merit of various methods for the detection of miRNA are compared in the present article. The current capabilities, limitations, and future challenges in miRNA detection and analysis based on nanomaterials are also addressed.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/c9an00081jDOI Listing
May 2019

Metal-organic framework derived small sized metal sulfide nanoparticles anchored on N-doped carbon plates for high-capacity energy storage.

Dalton Trans 2019 Apr;48(14):4712-4718

College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China.

ZnS nanoparticles wrapped in N-doped mesoporous carbon nanosheets are prepared from metal-organic frameworks through a simple co-pyrolysis process. In the composite interface, ZnS nanoparticles are directly loaded on the carbon and form a mesoporous structure, thus providing fast electron and ion dual transfer routes, and efficient electrode reaction kinetics. In addition, the heteroatom N-doping and the small-sized ZnS with embedded carbon can provide more active sites and stable electrode kinetics for electrochemical reactions. As a consequence, this material shows a capacity of 160 mA h g-1 at 1 A g-1, and retains 92.9% of its initial capacity after cycling for 1000 times at 5 A g-1. In the assembled asymmetric device, an energy density of 45.5 W h kg-1 is obtained. Such superior properties and facile production process of the metal-organic framework-derived composite show great potential in energy storage.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/c9dt00195fDOI Listing
April 2019

Cycling profile of layered MgAlO/reduced graphene oxide composite for asymmetrical supercapacitor.

J Colloid Interface Sci 2019 Mar 12;539:38-44. Epub 2018 Dec 12.

College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China. Electronic address:

To improve the ineluctable agglomeration and weak inherent conductivity of MgAlO electrode materials, MgAlO/rGO composite is synthesized by a facile method and it shows large specific surface area and enhanced conductivity. Its particular framework can availably hold back the aggregation of MgAlO and restacking of rGO, and accelerate reversible redox reactions. The MgAlO/rGO composite shows a specific capacity of 536.6 F/g at 1 A/g (257.3 F/g at 40 A/g) and retains 96.9% after 10,000 cycles at 5 A/g. An asymmetric supercapacitor is developed with MgAlO/rGO composite and activated carbon. An energy density of 16.2 Wh/kg is obtained at a power density of 400 kW/kg. Additionally, this device has successfully lighted up a LED, demonstrating its promising application in energy storage.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jcis.2018.12.045DOI Listing
March 2019

Ultrasensitive determination of thrombin by using an electrode modified with WSe and gold nanoparticles, aptamer-thrombin-aptamer sandwiching, redox cycling, and signal enhancement by alkaline phosphatase.

Mikrochim Acta 2018 10 9;185(11):502. Epub 2018 Oct 9.

College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang, 464000, China.

A sensitive aptamer/protein binding-triggered sandwich assay for thrombin is described. It is based on electrochemical-chemical-chemical redox cycling using a glassy carbon electrode (GCE) that was modified with WSe and gold nanoparticles (AuNPs). The AuNPs are linked to thrombin aptamer 1 via Au-S bonds. Thrombin is first captured by aptamer 1 and then sandwiched through the simultaneous interaction with AuNPs modified with thrombin-specific aptamer 2 and signalling probe. Subsequently, the DNA-linked AuNP hybrids result in the capture of streptavidin-conjugated alkaline phosphatase onto the modified GCE through the specific affinity reaction for further signal enhancement. As a result, a linear range of 0-1 ng mL and a detection limit as low as 190 fg mL are accomplished. The specificity for thrombin is excellent. Conceivably, this strategy can be easily expanded to other proteins by using the appropriate aptamer. Graphical abstract Schematic presentation of an electrochemical biosensor for thrombin based on WSe and gold nanoparticles, aptamer-thrombin-aptamer sandwiching, redox cycling, and signal enhancement by alkaline phosphatase.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00604-018-3028-7DOI Listing
October 2018

Electrochemical biosensor based on Se-doped MWCNTs-graphene and Y-shaped DNA-aided target-triggered amplification strategy.

Colloids Surf B Biointerfaces 2018 Dec 31;172:407-413. Epub 2018 Aug 31.

College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China. Electronic address:

A highly sensitive electrochemical biosensor for detection of platelet-derived growth factor-BB (PDGF-BB) is developed by using Se-doped multi-walled carbon nanotubes (MWCNTs)-graphene hybrids as electrode supporting substrate, hemin/G-quadruplex as trace labels and Y-shaped DNA-aided target recycling as signal magnifier. The aptamer-containing hairpin probes were first immobilized on the electrode. When target PDGF-BB was added, the aptamer binded PDGF-BB to trigger catalytic assembly of two other hairpins to form many G-quadruplex Y-junction DNA structures, which released PDGF-BB to again bind the intact aptamer to initiate another assembly cycle. G-quadruplex/hemin complexes were produced when hemin was added to generate substantially amplified current output. The developed assay showed a linear range toward PDGF-BB from 0.1 pM to 10 nM with a detection limit of 27 fM (S/N = 3). The method showed excellent specificity and repeatability, and could be expediently applied for sensitive detection of other molecules by simply changing the aptamers.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.colsurfb.2018.08.064DOI Listing
December 2018

High-performance supercapacitor based on three-dimensional flower-shaped LiTiO-graphene hybrid and pine needles derived honeycomb carbon.

J Colloid Interface Sci 2018 Nov 5;529:171-179. Epub 2018 Jun 5.

College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China. Electronic address:

A three-dimensional (3D) flower-shaped LiTiO-graphene (Gr) hybrid micro/nanostructures and pine needles derived carbon nanopores (PNDCN) has been prepared by using the effective hydrothermal process. Due to the unique micro/nanostructures which can provide abundant surface active sites, the obtained 3D LiTiO-Gr displays a high specific capacitance of 706.52 F g at 1 A g. The prepared PNDCN also exhibits high specific capacitance of 314.50 F g at 1 A g benefiting from its interconnected honeycomb-like hierarchical and open structure, which facilitates the diffusion and reaction of electrolyte ions and enables an isotropic charging/discharging process. An asymmetric supercapacitor utilizing LiTiO-Gr as positive electrode and PNDCN as negative electrode has been fabricated, it delivers a high energy density of 35.06 Wh kg at power density of 800.08 W kg and outstanding cycling stability with 90.18% capacitance retention after 2000 cycles. The fabrication process presented in this work is facile, cost-effective, and environmentally benign, offering a feasible solution for manufacturing next-generation high-performance energy storage devices.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jcis.2018.06.007DOI Listing
November 2018

Ultrasensitive supersandwich-type biosensor for enzyme-free amplified microRNA detection based on N-doped graphene/Au nanoparticles and hemin/G-quadruplexes.

J Mater Chem B 2018 Apr 26;6(14):2134-2142. Epub 2018 Mar 26.

College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China.

A simple, enzyme-free supersandwich-type biosensor is fabricated for the ultrasensitive detection of microRNAs (miRNAs) using N-doped graphene/Au nanoparticles (NG-AuNPs) and hemin/G-quadruplexes. In the proposed strategy, AuNPs are deposited on the surface of a MoSe modified electrode to immobilize the thiol-modified hairpin probe through the strong Au-S bond. When the target miRNA is added, capture DNA hybridizes with it and unfolds its stem-and-loop structure. The NG-AuNP hybrids are the main amplification element and are modified by hybridization with assistance DNA and the terminus of capture DNA, resulting in the formation of the supersandwich structure. The assistance DNA is embedded into the hemin/G-quadruplex complexes in the presence of hemin and K to provide an exceptional current signal for the detection of miRNAs. Under the optimized experimental conditions, a detection limit of 0.17 fM is obtained with a linear range of 10 fM-1 nM. In addition, the present biosensor shows outstanding selectivity towards mismatched miRNAs. This biosensor platform successfully realized the combination of the signal amplification technique with the supersandwich structure, providing a promising approach for the detection of miRNA-21 in practical applications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/c8tb00061aDOI Listing
April 2018

A yolk-shell VO structure assembled from ultrathin nanosheets and coralline-shaped carbon as advanced electrodes for a high-performance asymmetric supercapacitor.

Dalton Trans 2018 Feb;47(7):2256-2265

College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China.

Various VO three-dimensional nanostructures are synthesized using a facile template-free hydrothermal method and evaluated for use as supercapacitor electrode materials. As a result, the yolk-shell structure assembled from ultrathin nanosheets shows the best electrochemical performance, with a specific capacitance of 704.17 F g at 1.0 A g and a high capacity retention of 89% over 4000 cycles at 3.0 A g. In addition, a continuous three-dimensional porous coralline-shaped carbon is synthesized from osmanthus and has a large Brunauer-Emmett-Teller surface area of 2840.88 m g. Then, an asymmetric supercapacitor is developed using the as-prepared yolk-shell VO as a positive electrode and the osmanthus derived coralline-shaped carbon as a negative electrode. This exhibits an energy density of 29.49 W h kg at a power density of 800 W kg with a good cycling performance that retains 90.6% of its initial capacity after 2000 cycles at 3.0 A g. Furthermore, two cells in series can easily brightly light up a light-emitting diode (3 V), further demonstrating the great potential of the prepared materials for high-performance supercapacitor devices.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/c7dt04660jDOI Listing
February 2018

An electrochemical microRNA sensing platform based on tungsten diselenide nanosheets and competitive RNA-RNA hybridization.

Analyst 2017 Dec;142(24):4843-4851

College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China.

In this work, we report an ultrasensitive electrochemical biosensor for microRNA-21 (miRNA-21) detection by using a competitive RNA-RNA hybridization configuration. A biotinylated miRNA of the self-same sequence with the target miRNA is mixed with the samples, and allowed competition with the target miRNA for a thiolated RNA probe immobilized onto a tungsten diselenide (WSe) nanosheet modified electrode. Thereafter the current response is obtained by forming the hybridized biotinylated miRNA with streptavidin-horseradish peroxidase (HRP) conjugates to catalyze the HO + hydroquinone (HQ) system. Benefiting from the high specific surface area of WSe nanosheets, the competitive hybridization configuration and the signal amplification of the HO + HQ detection system, the proposed assay exhibits a wide linear range of 0.0001-100 pM towards target miRNA with a detection limit of 0.06 fM (S/N = 3), and shows excellent discrimination ability for base-mismatched miRNA sequences. Therefore, the designed platform has promising prospects for the detection of miRNA in biomedical research and early clinical diagnosis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/c7an01244fDOI Listing
December 2017

Tetrahedral DNA probe coupling with hybridization chain reaction for competitive thrombin aptasensor.

Biosens Bioelectron 2018 Feb 15;100:274-281. Epub 2017 Sep 15.

College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China; Institute for Conservation and Utilization of Agro-bioresources in Dabie Mountains, Xinyang Normal University, Xinyang 464000, China.

A novel competitive aptasensor for thrombin detection is developed by using a tetrahedral DNA (T-DNA) probe and hybridization chain reaction (HCR) signal amplification. Sulfur and nitrogen co-doped reduced graphene oxide (SN-rGO) is firstly prepared by a simple reflux method and used for supporting substrate of biosensor. Then, T-DNA probe is modified on the electrode by Au-S bond and a competition is happened between target thrombin and the complementary DNA (cDNA) of aptamer. The aptamer binding to thrombin forms an aptamer-target conjugate and make the cDNA remained, and subsequently hybridizes with the vertical domain of T-DNA. Finally, the cDNAs trigger HCR, which results in a great current response by the catalysis of horseradish peroxidase to the hydrogen peroxide + hydroquinone system. For thrombin detection, the proposed biosensor shows a wide linearity range of 10-10M and a low detection limit of 11.6fM (S/N = 3), which is hopeful to apply in biotechnology and clinical diagnosis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bios.2017.09.022DOI Listing
February 2018

Ultrasensitive electrochemical sensing platform based on graphene wrapping SnO nanocorals and autonomous cascade DNA duplication strategy.

Talanta 2017 Dec 14;175:168-176. Epub 2017 Jul 14.

College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China; Henan Province Key Laboratory of Utilization of Non-metallic Mineral in the South of Henan, Xinyang Normal University, Xinyang 464000, China; Institute for Conservation and Utilization of Agro-bioresources in Dabie Mountains, Xinyang Normal University, Xinyang 464000, China.

In this work, a sensitive, universal and reusable electrochemical biosensor based on stannic oxide nanocorals-graphene hybrids (SnO NCs-Gr) is developed for target DNA detection by using two kinds of DNA enzymes for signal amplification through an autonomous cascade DNA duplication strategy. A hairpin probe is designed composing of a projecting part at the 3'-end as identification sequence for target, a recognition site for nicking endonuclease, and an 18-carbon shim to stop polymerization process. The designed DNA duplication-incision-replacement process is handled by KF polymerase and endonuclease, then combining with gold nanoparticles as signal carrier for further signal amplification. In the detection system, the electrochemical-chemical-chemical procedure, which uses ferrocene methanol, tris(2-carboxyethyl)phosphine and l-ascorbic acid 2-phosphate as oxidoreduction neurogen, deoxidizer and zymolyte, separately, is applied to amplify detection signal. Benefiting from the multiple signal amplification mechanism, the proposed sensor reveals a good linear connection between the peak current and logarithm of analyte concentration in range of 0.0001-1 × 10molL with a detection limit of 1.25 × 10molL (S/N=3). This assay also opens one promising strategy for ultrasensitive determination of other biological molecules for bioanalysis and biomedicine diagnostics.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.talanta.2017.07.042DOI Listing
December 2017

Recent advances in signal amplification strategy based on oligonucleotide and nanomaterials for microRNA detection-a review.

Biosens Bioelectron 2018 Jan 18;99:612-624. Epub 2017 Aug 18.

College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China; Henan Province Key Laboratory of Utilization of Non-metallic Mineral in the South of Henan, Xinyang Normal University, Xinyang 464000, China.

MicroRNAs (MiRNAs) play multiple crucial regulating roles in cell which can regulate one third of protein-coding genes. MiRNAs participate in the developmental and physiological processes of human body, while their aberrant adjustment will be more likely to trigger diseases such as cancers, kidney disease, central nervous system diseases, cardiovascular diseases, diabetes, viral infections and so on. What's worse, for the detection of miRNAs, their small size, high sequence similarity, low abundance and difficult extraction from cells impose great challenges in the analysis. Hence, it's necessary to fabricate accurate and sensitive biosensing platform for miRNAs detection. Up to now, researchers have developed many signal-amplification strategies for miRNAs detection, including hybridization chain reaction, nuclease amplification, rolling circle amplification, catalyzed hairpin assembly amplification and nanomaterials based amplification. These methods are typical, feasible and frequently used. In this review, we retrospect recent advances in signal amplification strategies for detecting miRNAs and point out the pros and cons of them. Furthermore, further prospects and promising developments of the signal-amplification strategies for detecting miRNAs are proposed.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bios.2017.08.036DOI Listing
January 2018

Molybdenum disulfide sphere-based electrochemical aptasensors for protein detection.

J Mater Chem B 2017 Jul 22;5(27):5362-5372. Epub 2017 Jun 22.

College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China.

In this work, we report the development of an ultrasensitive sandwich-type electrochemical aptasensor for protein detection. The aptasensor is fabricated by using nitrogen-doped graphene oxide (N-GO) and Au nanoparticles (AuNPs) as sensing substrates, molybdenum disulfide (MoS) spheres as the hybridization chain reaction (HCR) platform, and thrombin as the model protein. When the hybridization reaction is initiated through two biotinylated hairpin probes, vast horseradish peroxidases are immobilized on the long duplex by the biotin-avidin reaction. An electrochemical-chemical-chemical redox cycling reaction then takes place in the detection system, which contains p-dihydroxybenzene, ferrocene carboxylate and tris(2-carboxyethyl)phosphine. Benefiting from the good conductivity and high specific surface area of N-GO/AuNPs and MoS spheres, signal amplification of the HCR and detection system, and excellent selectivity of the aptamer and sandwich-type strategy, the proposed assay shows a wide linear range of 10 fM-0.1 nM towards thrombin with a detection limit of 27 aM (S/N = 3) along with clear distinction from different proteins. The proposed assay is successfully used to detect thrombin in human serum, which would have promising prospects for disease diagnosis and therapy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/c7tb01276dDOI Listing
July 2017

NiCo S Materials for Supercapacitor Applications.

Chem Asian J 2017 Aug 19;12(16):1969-1984. Epub 2017 Jun 19.

College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang, 464000, P.R. China.

Cobalt-nickel sulfide (NiCo S ) shows extensive potential for innovative photoelectronic and energetic materials owing to distinctive physical and chemical properties. In this review, representative strategies for the fabrication and application of NiCo S and composite nanostructures are outlined for supercapacitors, with the aim of promoting the development of NiCo S and their composites in the supercapacitor field through an analysis and comparison of diverse nanostructures. A brief introduction into the structures, properties, and morphologies are presented. Further prospects and promising developments of the materials in the supercapacitor field are also proposed.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/asia.201700461DOI Listing
August 2017

Recent advances in transition-metal dichalcogenides based electrochemical biosensors: A review.

Biosens Bioelectron 2017 Nov 8;97:305-316. Epub 2017 Jun 8.

School of Physics and Electronic Engineering, Xinyang Normal University, Xinyang 464000, China.

Layered transition metal dichalcogenides (TMDCs) comprise a category of two-dimensional (2D) materials that offer exciting properties, including large surface area, metallic and semi-conducting electrical capabilities, and intercalatable morphologies. Biosensors employ biological molecules to recognize the target and utilize output elements which can translate the biorecognition event into electrical, optical or mass-sensitive signals to determine the quantities of the target. TMDCs nanomaterials have been widely applied in various electrochemical biosensors with high sensitivity and selectivity. The marriage of TMDCs and electrochemical biosensors has created many productive sensing strategies for applications in the areas of clinical diagnosis, environmental monitoring and food safety. In recent years, an increasing number of TMDCs-based electrochemical biosensors are reported, suggesting TMDCs offers new possibilities of improving the performance of electrochemical biosensors. This review summarizes recent advances in electrochemical biosensors based on TMDCs for detection of various inorganic and organic analytes in the last five years, including glucose, proteins, DNA, heavy metal, etc. In addition, we also point out the challenges and future perspectives related to the material design and development of TMDCs-based electrochemical biosensors.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bios.2017.06.011DOI Listing
November 2017

General Strategy for Controlled Synthesis of NiP/Carbon and Its Evaluation as a Counter Electrode Material in Dye-Sensitized Solar Cells.

ACS Appl Mater Interfaces 2017 May 17;9(21):17949-17960. Epub 2017 May 17.

College of Chemistry and Chemical Engineering, Xinyang Normal University , Xinyang 464000, China.

Hydrothermal treatment of nickel acetate and phosphoric acid aqueous solution followed with a carbothermal reduction assisted phosphorization process using sucrose as the carbon source for the controlled synthesis of NiP/C was successfully realized for the first time. The critical synthesis factors, including reduction temperature, phosphorus/nickel ratio, pH, and sucrose amount were systematically investigated. Remarkably, the carbon serves as a reducer and plays a determinative role in the transformation of NiPO into NiP/C. The synthesis strategy is divided into four distinguishable stages: (1) hydrothermal preparation of Ni(PO)·8HO precursor for stabilizing P sources; (2) dimerization of Ni(PO)·8HO into more thermal stable NiPO amorphous phase along with the generation of NiO; (3) carbothermal reduction and phosphidation of NiO into NiP (0 ≤ y/x ≤ 0.5); and (4) further phosphidation of mixed-phase NiP and carbothermal reduction of NiPO into single-phase NiP. The resultant NiP, the highly active phase in electrocatalysis, was applied as counter electrode in a dye-sensitized solar cell (DSSC). The DSSC based on NiP with 10.4 wt.% carbon delivers a power conversion efficiency of 9.57%, superior to that of state-of-the-art Pt-based cell (8.12%). The abundant Ni and P active sites and the metal-like conductivity account for its outstanding catalytic performance.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.7b03541DOI Listing
May 2017

Ultrasensitive electrochemical biosensing platform based on spherical silicon dioxide/molybdenum selenide nanohybrids and triggered Hybridization Chain Reaction.

Biosens Bioelectron 2017 Aug 30;94:616-625. Epub 2017 Mar 30.

College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China; Institute for Conservation and Utilization of Agro-bioresources in Dabie Mountains, Xinyang Normal University, Xinyang 464000, China.

An ultrasensitive sandwich-type electrochemical biosensor for DNA detection is developed based on spherical silicon dioxide/molybdenum selenide ([email protected]) and graphene oxide-gold nanoparticles (GO-AuNPs) hybrids as carrier triggered Hybridization Chain Reaction (HCR) coupling with multi-signal amplification. The proposed sensoring assay utilizes a spherical [email protected]/AuNPs as sensing platform and GO-AuNPs hybrids as carriers to supply vast binding sites. HO+HQ system is used for DNA detection and HCR as the signal and selectivity enhancer. The sensor is designed in sandwich type to increase the specificity. As a result, the present biosensor exhibits a good dynamic range from 0.1fM to 100pM with a low detection limit of 0.068fM (S/N=3). This work shows a considerable potential for quantitative detection of DNA in early clinical diagnostics.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bios.2017.03.058DOI Listing
August 2017

Au nanoparticles/hollow molybdenum disulfide microcubes based biosensor for microRNA-21 detection coupled with duplex-specific nuclease and enzyme signal amplification.

Biosens Bioelectron 2017 Mar 21;89(Pt 2):989-997. Epub 2016 Oct 21.

College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China.

An ultrasensitive electrochemical biosensor for detecting microRNAs is fabricated based on hollow molybdenum disulfide (MoS) microcubes. Duplex-specific nuclease, enzyme and electrochemical-chemical-chemical redox cycling are used for signal amplification. Hollow MoS microcubes constructed by ultrathin nanosheets are synthesized by a facile template-assisted strategy and used as supporting substrate. For biosensor assembling, biotinylated ssDNA capture probes are first immobilized on Au nanoparticles (AuNPs)/MoS modified electrode in order to combine with streptavidin-conjugated alkaline phosphatase (SA-ALP). When capture probes hybridize with miRNAs, duplex-specific nuclease cleaves the formative duplexes. At the moment, the biotin group strips from the electrode surface and SA-ALP is incapacitated to attach onto electrode. Then, ascorbic acids induce the electrochemical-chemical-chemical redox cycling to produce electrochemical response in the presence of ferrocene methanol and tris (2-carboxyethyl) phosphine. Under optimum conditions, the proposed biosensor shows a good linear relationship between the current variation and logarithm of the microRNAs concentration ranging from 0.1fM to 0.1pM with a detection limit of 0.086fM (S/N=3). Furthermore, the biosensor is successfully applied to detect target miRNA-21 in human serum samples.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bios.2016.10.051DOI Listing
March 2017

Preparation of layered graphene and tungsten oxide hybrids for enhanced performance supercapacitors.

Dalton Trans 2016 Nov;45(43):17439-17446

College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China.

Tungsten oxide (WO), which was originally poor in capacitive performance, is made into an excellent electrode material for supercapacitors by dispersing it on graphene (Gr). The obtained Gr-WO hybrids are characterized by X-ray diffraction, Raman spectroscopy, high-resolution transmission electron microscopy and scanning electron microscopy techniques, and evaluated as electrode materials for high-performance supercapacitors by cyclic voltammetry, galvanostatic charge-discharge curves and electrochemical impedance spectroscopy. A great improvement in specific capacitance is achieved with the present hybrids, from 255 F g for WO nanoparticles to 580 F g for Gr-WO hybrids (scanned at 1 A g in 2 M KOH over a potential window of 0 to 0.45 V). The Gr-WO hybrid exhibits an excellent high rate capability and good cycling stability with more than 92% capacitance retention over 1000 cycles at a current density of 5 A g. The enhancement in supercapacitor performance of Gr-WO is not only attributed to its unique nanostructure with large specific surface area, but also its excellent electro-conductivity, which facilitates efficient charge transport and promotes electrolyte diffusion. As a whole, this work indicates that Gr-WO hybrids are a promising electrode material for high-performance supercapacitors.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/c6dt03719dDOI Listing
November 2016

Ultrasensitive electrochemical sensing platform for microRNA based on tungsten oxide-graphene composites coupling with catalyzed hairpin assembly target recycling and enzyme signal amplification.

Biosens Bioelectron 2016 Dec 23;86:337-345. Epub 2016 Jun 23.

College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China.

An ultrasensitive electrochemical biosensor for microRNA (miRNA) is developed based on tungsten oxide-graphene composites coupling with catalyzed hairpin assembly target recycling and enzyme signal amplification. WO3-Gr is prepared by a simple hydrothermal method and then coupled with gold nanoparticles to act as a sensing platform. The thiol-terminated capture probe H1 is immobilized on electrode through Au-S interaction. In the presence of target miRNA, H1 opens its hairpin structure by hybridization with target miRNA. This hybridization can be displaced from the structure by another stable biotinylated hairpin DNA (H2), and target miRNA is released back to the sample solution for next cycle. Thus, a large amount of H1-H2 duplex is produced after the cyclic process. At this point, a lot of signal indicators streptavidin-conjugated alkaline phosphatase (SA-ALP) are immobilized on the electrode by the specific binding of avidin-biotin. Then, thousands of ascorbic acid, which is the enzymatic product of ALP, induces the electrochemical-chemical-chemical redox cycling to produce a strongly electrochemical response in the presence of ferrocene methanol and tris (2-carboxyethyl) phosphine. Under the optimal experimental conditions, the established biosensor can detect target miRNA down to 0.05fM (S/N=3) with a linear range from 0.1fM to 100pM, and discriminate target miRNA from mismatched miRNA with a high selectivity.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bios.2016.06.057DOI Listing
December 2016

A layered tungsten disulfide/acetylene black composite based DNA biosensing platform coupled with hybridization chain reaction for signal amplification.

J Mater Chem B 2016 Feb 21;4(6):1186-1196. Epub 2016 Jan 21.

College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China.

A 2-dimensional tungsten disulfide-acetylene black (WS-AB) composite is synthesized by a simple hydrothermal method to achieve excellent electrochemical properties for applications as a DNA biosensor. The biosensor is fabricated based on the Au nanoparticles (AuNPs) and WS-AB composite modified electrode, which subsequently is used to couple with a capture probe by an Au-S bond, then modified with target DNA, auxiliary DNA and bio-H1-bio-H2 (H1-H2) to perform hybridization chain reaction for signal amplification. Herein, two DNA hairpins H1 and H2 are opened by the recognition probe. The nicked double helices from hybridization chain reaction are used to immobilize horseradish peroxidase enzymes via biotin-avidin reaction, which produces signal-amplification detection of target DNA through the catalytic reaction of the hydrogenperoxide + hydroquinone system. Under optimum conditions, the as-prepared biosensor shows a good linear relationship between the current value and logarithm of the target DNA concentration ranging from 0.001 pM to 100 pM and a detection limit as low as 0.12 fM. Moreover, the fabricated biosensor exhibits good selectivity to differentiate the one-base mismatched DNA sequence. This work will open a pathway for ultrasensitive detection of other biorecognition events and gene-related diseases based on layered WS-AB and hybridization chain reaction.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/c5tb02214bDOI Listing
February 2016

Ultrasensitive biosensing platform based on layered vanadium disulfide-graphene composites coupling with tetrahedron-structured DNA probes and exonuclease III assisted signal amplification.

J Mater Chem B 2015 Nov 21;3(41):8180-8187. Epub 2015 Sep 21.

College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China.

Recently, two-dimensional layered transition metal dichalcogenide materials have been very fascinating in electrochemical fields due to their unique electronic and electrochemical properties. In the present work, a novel VS-graphene (VS-GR) composite is firstly prepared by a facile one-step hydrothermal method. A sensitive, universal and label-free electrochemical aptamer-based sensor (aptasensor) is then designed to detect the platelet-derived growth factor BB (PDGF-BB) based on VS-GR coupled with Exo III-aided autocatalytic signal amplification. In the absence of PDGE-BB, the aptamer hybridized with the complementary DNA (cDNA) and the single-strand signal DNA labeled with biotin at 5' end cannot be cleaved by the Exo III. When the above mixture is applied on the tetrahedron-structured probe (T-DNA) modified AuNP/VS-GR electrode, signal DNA hybridizes with the protruding of T-DNA and a large number of avidin-HRP are adsorbed on the modified electrode by the specific binding of avidin-biotin, which leads to a strong current response by the catalysis of HRP to the mixture of hydrogen peroxide and hydroquinone (HO + HQ). However, in the presence of PDGF-BB, the aptamer is preferentially associated with PDGF-BB, which facilitates the formation of double-strand DNA between cDNA and signal DNA. The duplex DNA is digested by Exo III from the 3' blunt terminus of signal DNA and the cDNA is released. The liberative cDNA then reacts with the remaining signal DNA in the solution to perform a new cleavage process. Finally, the cyclic hybridization-hydrolysis process leads to a great decrease of biotin on the electrode, thus reducing the quantity of signal molecule HRP and causing an obvious decline of current response. This proposed protocol exhibits high specificity, good sensitivity, and good stability for PDGF-BB detection. Moreover, the aptasensor can be applied as a universal assay for the detection of various targets including proteins, DNAs, and metal ions.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/c5tb01239bDOI Listing
November 2015

Ultrasensitive sensing platform for platelet-derived growth factor BB detection based on layered molybdenum selenide-graphene composites and Exonuclease III assisted signal amplification.

Biosens Bioelectron 2016 Mar 12;77:69-75. Epub 2015 Sep 12.

College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China.

A highly sensitive and ultrasensitive electrochemical aptasensor for platelet-derived growth factor BB (PDGF-BB) detection is fabricated based on layered molybdenum selenide-graphene (MoSe2-Gr) composites and Exonuclease III (Exo III)-aided signal amplification. MoSe2-Gr is prepared by a simple hydrothermal method and used as a promising sensing platform. Exo III has a specifical exo-deoxyribonuclease activity for duplex DNAs in the direction from 3' to 5' terminus, however its activity is limited on the duplex DNAs with more than 4 mismatched terminal bases at 3' ends. Herein, aptamer and complementary DNA (cDNA) sequences are designed with four thymine bases on 3' ends. In the presence of target protein, the aptamer associates with it and facilitates the formation of duplex DNA between cDNA and signal DNA. The duplex DNA then is digested by Exo III and releases cDNA, which hybridizes with signal DNA to perform a new cleavage process. Nevertheless, in the absence of target protein, the aptamer hybridizes with cDNA will inhibit the Exo III-assisted nucleotides cleavage. The signal DNA then hybridizes with capture DNA on the electrode. Subsequently, horse radish peroxidase is fixed on electrode by avidin-biotin reaction and then catalyzes hydrogen peroxide and hydroquinone to produce electrochemical response. Therefore, a bridge can be established between the concentration of target protein and the degree of the attenuation of the obtained signal, providing a quantitative measure of target protein with a broad detection range of 0.0001-1 nM and a detection limit of 20 fM.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bios.2015.09.026DOI Listing
March 2016