Publications by authors named "Ke Xue"

158 Publications

Insight into pyrolysis mechanism of 1,2-propylene glycol: Based on density functional theory and wavefunction analysis.

J Mol Graph Model 2022 Nov 20;116:108277. Epub 2022 Jul 20.

School of Chemistry and Chemical Engineering, Southeast University, Nanjing, Jiangsu, 211189, PR China. Electronic address:

The multiple thermal decomposition mechanisms of 1,2-propylene glycol are studied through theoretical calculation and experiment, including carbon chain break, dehydrogenation and dehydration mechanism. The wavefunction is employed to analyze the decomposition process from a micro perspective. DLPNO-CCSD(T)/CBS method is engaged in establishing potential energy surface. The results reveal that the dehydration and carbon chain break mechanism are the primary pyrolysis paths, and the former is the dominant pyrolysis mechanism at low temperature, while the latter is applicable at the high temperature. The pyrolysis products are mainly acetaldehyde, propanal and acetone, which is consistent with experimental results. Besides, the comparison results of 1,2-propylene glycol and glycerol pyrolysis products indicate that the increment of hydroxyls are conducive to the generation of carbonyl compounds during the polyol thermal decomposition. This work is aimed to comprehensively investigate the pyrolysis mechanism of 1,2-propylene glycol and provide the valuable thermodynamics and kinetic data for developing efficient polyol pyrolysis technology. Furthermore, it provides a reference for choosing low-toxic tobacco humectant.
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http://dx.doi.org/10.1016/j.jmgm.2022.108277DOI Listing
November 2022

Correction to: Enhanced tryptophan-kynurenine metabolism via indoleamine 2,3-dioxygenase 1 induction in dermatomyositis.

Clin Rheumatol 2022 Jul 28. Epub 2022 Jul 28.

Department of Dermatology, Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine, No. 197, Rui Jin 2nd Road, Shanghai, 200025, China.

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http://dx.doi.org/10.1007/s10067-022-06317-6DOI Listing
July 2022

Improved Durability of High-Performance Intermediate-Temperature Solid Oxide Fuel Cells with a Ba-Doped LaSrCoFeO Cathode.

ACS Appl Mater Interfaces 2022 Jul 13. Epub 2022 Jul 13.

School of Engineering, The University of Western Australia, Perth, WA 6009, Australia.

As a device for direct conversion of chemical energy into electrical energy, the solid oxide fuel cell (SOFC) contributes positively to the sustainable development strategy. However, the commercialization of fuel cells is still impeded by severe cathode degradation caused by its limited stability at operating temperatures and being prone to Cr-poisoning from Cr-containing alloy interconnectors commonly used in these cells. This paper reports the development of a high-durability Ba-doped LSCF(LaSrCoFeO) cathode material under realistic fuel cell operating conditions in the presence of the Cr alloy. In particular, when tested in a symmetrical cell constructed of Ba-doped LSCF, the polarization resistance of the cell remains very low at 0.06 Ω cm after being tested at 800 °C for 120 h exposed to Cr in 3% humidified air. In contrast, for the undoped LSCF under the same testing conditions, the polarization resistance of the cell increases ∼10 times from 0.22 Ω cm of the pristine cell to 2.18 Ω cm after Cr-exposure testing. Furthermore, when tested in an anode-supported complete cell as a cathode under typical SOFC operation conditions at 750 °C, the cell with the Ba-doped LSCF cathode displays significantly low degradation rates of 0.00056% h (without Cr) and 0.00310% h (with Cr); both are much lower than that of the cell using the undoped LSCF cathode (0.00124% h without Cr and 0.01082% h with Cr). This enhanced durability and Cr-tolerance exhibited by the Ba-doped LSCF cathode stem from its higher crystal structure stability and improved chemical resistance compared to undoped LSCF.
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http://dx.doi.org/10.1021/acsami.2c05149DOI Listing
July 2022

Blocking the Aryl Hydrocarbon Receptor Alleviates Myocardial Ischemia/Reperfusion Injury in Rats.

Curr Med Sci 2022 Jul 5. Epub 2022 Jul 5.

Department of Anesthesiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.

Objective: Restoring the blood perfusion of ischemic heart tissues is the main treatment for myocardial ischemia. However, the accompanying myocardial ischemia reperfusion injury (IRI) would aggravate myocardial damage. Previous studies have confirmed that aryl hydrocarbon receptor (AhR) is closely correlated to kidney and intestinal IRI. The present study aimed to explore the relationship between AhR and myocardial IRI.

Methods: An oxygen glucose deprivation/reoxygenation (OGD/R) model of H9c2 cells and an ischemia/reperfusion (I/R) model of Sprague-Dawley rat myocardium were established. OGD/R cells and myocardial IRI rats were treated with different concentrations of the AhR antagonist CH-223191 or agonist 6-formylindolo[3,2-b] carbazole (FICZ). Under the conditions of normoxia and hypoxia/reoxygenation, the activity of cardiomyocytes, lactate dehydrogenase (LDH) and cell reactive oxygen species (ROS) were detected. In rats, myocardial pathological damage and markers of myocardial injury were detected.

Results: According to the results of the cell viability, LDH and ROS tests in vitro, both CH-223191 and FICZ showed no myocardial protection under OGD/R conditions. However, the histological staining and analysis of myocardial injury marker LDH in vitro revealed that CH-223191 could significantly reduce the myocardial IRI.

Conclusion: AhR exhibited a different effect on myocardial IRI in vitro and in vivo. In vivo, CH-223191 could significantly alleviate the myocardial IRI, suggesting that inhibition of AhR may play a role in myocardial protection, and AhR may serve as a potential treatment target for myocardial IRI.
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http://dx.doi.org/10.1007/s11596-022-2601-9DOI Listing
July 2022

Enhanced tryptophan-kynurenine metabolism via indoleamine 2,3-dioxygenase 1 induction in dermatomyositis.

Clin Rheumatol 2022 Jul 1. Epub 2022 Jul 1.

Department of Dermatology, Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine, No. 197, Rui Jin 2nd Road, Shanghai, 200025, China.

Objectives: Extrahepatic tryptophan (Trp)-kynurenine (Kyn) metabolism via indoleamine 2,3-dioxygenase 1 (IDO1) induction was found to be associated with intrinsic immune regulation. However, the Trp-Kyn metabolism-associated immune regulation in dermatomyositis (DM) remains unknown. Therefore, we aimed to investigate the clinical relevance of the Trp-Kyn metabolism via IDO1 induction in DM.

Methods: Liquid chromatography-mass spectrometry (HPLC-MS) was used to examine the serum Kyn and Trp concentrations in DM. In addition, we used X-tile software to determine the optimal cutoff value of the Kyn/Trp ratio, a surrogate marker for Trp-Kyn metabolism. Spearman analysis was performed to evaluate the association of Trp-Kyn metabolism with muscle enzymes and inflammatory markers.

Results: DM patients had significantly higher serum Kyn/Trp ratio (× 10) when compared with the healthy controls. The serum Kyn/Trp ratio was positively correlated with the levels of muscle enzymes and inflammatory markers. In addition, the serum Kyn/Trp ratio significantly decreased (36.89 (26.00-54.00) vs. 25.00 (18.00-37.00), P = 0.0006) after treatment. DM patients with high serum Kyn/Trp ratio had a significantly higher percentage of muscle weakness symptoms (62.5% vs. 20.0%, P = 0.019) and higher levels of LDH (316.0 (236.0-467.0) vs. 198.0 (144.0-256.0), P = 0.004) and AST (56.5 (35.0-92.2) vs. 23.0 (20.0-36.0), P = 0.002)) than those with low serum Kyn/Trp ratio. Multiple Cox regression analyses identified ln(Kyn/Trp) (HR 4.874, 95% CI 1.105-21.499, P = 0.036) as an independent prognostic predictor of mortality in DM.

Conclusions: DM patients with enhanced Trp-Kyn metabolism at disease onset are characterized by more severe disease status and poor prognosis. Intrinsic immune regulation function via enhanced Trp-Kyn metabolism by IDO1 induction may be a potential therapeutic target in DM. Key Points • HPLC-MS identified increased serum Kyn/Trp ratio in DM patients, which positively correlated with levels of muscle enzymes and inflammatory markers and was downregulated upon treatment. • Cox regression analyses identified ln(Kyn/Trp) as an independent prognostic predictor of mortality in DM. • Monitoring intrinsic immune regulation function should be considered a potential therapeutic target in DM patients.
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http://dx.doi.org/10.1007/s10067-022-06263-3DOI Listing
July 2022

Unveiling a Novel Source of Resistance to Bacterial Blight in Medicinal Wild Rice, .

Life (Basel) 2022 Jun 2;12(6). Epub 2022 Jun 2.

Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences, Yunnan Provincial Key Lab of Agricultural Biotechnology, Ministry of Agriculture, Kunming 650205, China.

Bacterial blight (BB) caused by pv. () is among the oldest known bacterial diseases found for rice in Asia. It is the most serious bacterial disease in many rice growing regions of the world. A total of 47 resistance (R) genes ( to ) have been identified. Nonetheless, these R genes could possibly be defeated to lose their qualitative nature and express intermediate phenotypes. The identification of sources of novel genetic loci regulating host plant resistance is crucial to develop an efficient control strategy. Wild ancestors of cultivated rice are a natural genetic resource contain a large number of excellent genes. Medicinal wild rice () belongs to the CC genome and is a well-known wild rice in south China. In this study, was crossed with cultivated rice HY-8 and their hybrids were screened for BB resistance genes deployed through natural selection in wild rice germplasm. The molecular markers linked to R genes for BB were used to screen the genomic regions in wild parents and their recombinants. The gene coding and promoter regions of major R genes were inconsistently found in and its progenies. showed resistance to all thirty inoculated strains with non-availability of various known R genes. The results indicated the presence of novel genomic regions for BB resistance in . The present study not only provides a reference to investigate medicinal rice for R gene(s) identification against BB but also identified it as a new breeding material for BB resistance.
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http://dx.doi.org/10.3390/life12060827DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9225586PMC
June 2022

Biomimetic doxorubicin/ginsenoside co-loading nanosystem for chemoimmunotherapy of acute myeloid leukemia.

J Nanobiotechnology 2022 Jun 14;20(1):273. Epub 2022 Jun 14.

Department of Pharmaceutics, China Pharmaceutical University, Xuanwumen Campus, No.24, Tongjiaxiang, Gulou District, Nanjing, 210009, Jiangsu province, China.

Background: Acute myeloid leukemia (AML) showed limited clinical therapeutic efficiency with chemotherapy for its multi-distributed lesions and hard-to-kill leukemia cells deep in the bone marrow.

Results: Here, a biomimetic nanosystem ([email protected]) based on platelet membrane (PM) coating and doxorubicin (DOX)/ginsenoside (Rg3) co-loading was developed to potentiate the local-to-systemic chemoimmunotherapy for AML. The PM was designed for long-term circulation and better leukemia cells targeting. The participation of Rg3 was proved to enhance the tumor sensitivity to DOX, thus initiating the anti-tumor immune activation and effectively combating the leukemia cells hiding in the bone marrow.

Conclusions: In conclusion, the strategy that combining immediate chemotherapy with long-term immunotherapy achieved improved therapeutic efficiency and prolonged survival, which provided a new perspective for the clinical treatment of AML.
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http://dx.doi.org/10.1186/s12951-022-01491-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9195256PMC
June 2022

Integration of Bioglass Into PHBV-Constructed Tissue-Engineered Cartilages to Improve Chondrogenic Properties of Cartilage Progenitor Cells.

Front Bioeng Biotechnol 2022 23;10:868719. Epub 2022 May 23.

Department of Plastic and Reconstructive Surgery, Shanghai 9th People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

The Poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) scaffold has proven to be a promising three-dimensional (3D) biodegradable and bioactive scaffold for the growth and proliferation of cartilage progenitor cells (CPCs). The addition of Bioglass into PHBV was reported to increase the bioactivity and mechanical properties of the bioactive materials. In the current study, the influence of the addition of Bioglass into PHBV 3D porous scaffolds on the characteristics of CPC-based tissue-engineered cartilages were compared. CPCs were seeded into 3D macroporous PHBV scaffolds and PHBV/10% Bioglass scaffolds. The CPC-scaffold constructs underwent 6 weeks chondrogenic induction culture and were then transplanted for another 6 weeks to evaluate the difference between the CPC-PHBV construct and CPC-PHBV/10% Bioglass construct . Compared with the pure PHBV scaffold, the PHBV/10% Bioglass scaffold has better hydrophilicity and a higher percentage of adhered cells. The CPC-PHBV/10%Bioglass construct produced much more cartilage-like tissues with higher cartilage-relative gene expression and cartilage matrix protein production and better biomechanical performance than the CPC-PHBV construct. The addition of Bioglass into 3D PHBV macroporous scaffolds improves the characteristics of CPC-based tissue-engineered cartilages .
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http://dx.doi.org/10.3389/fbioe.2022.868719DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9172278PMC
May 2022

A receptor-targeting AIE photosensitizer for selective bacterial killing and real-time monitoring of photodynamic therapy outcome.

Chem Commun (Camb) 2022 Jun 21;58(50):7058-7061. Epub 2022 Jun 21.

Key Laboratory of Functional Polymer Materials of Ministry of Education, State Key Laboratory of Medicinal Chemical Biology, Institute of Polymer Chemistry, College of Chemistry, Nankai University, Tianjin 300071, China.

A receptor-targeting AIE photosensitizer (CE-TPA) is synthesized by conjugating cephalothin with a cationic D-A type AIE photosensitizer for selective killing of Gram-positive bacteria over Gram-negative bacteria and normal mammalian cells. By virtue of the strong photosensitization capability, CE-TPA exhibits efficient killing against Gram-positive methicillin-resistant . More importantly, the photodynamic bactericidal outcome can be conveniently reflected in a real-time fashion by the polarity-sensitive property of CE-TPA.
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http://dx.doi.org/10.1039/d2cc02230cDOI Listing
June 2022

Sending-or-Not-Sending Twin-Field Quantum Key Distribution with a Passive Decoy-State Method.

Entropy (Basel) 2022 May 8;24(5). Epub 2022 May 8.

Key Lab of Broadband Wireless Communication and Sensor Network Technology, Ministry of Education, Nanjing 210003, China.

Twin-field quantum key distribution (TF-QKD) has attracted considerable attention because it can exceed the basic rate-distance limit without quantum repeaters. Its variant protocol, sending or not-sending quantum key distribution (SNS-QKD), not only fixes the security vulnerability of TF-QKD, but also can tolerate large misalignment errors. However, the current SNS-QKD protocol is based on the active decoy-state method, which may lead to side channel information leakage when multiple light intensities are modulated in practice. In this work, we propose a passive decoy-state SNS-QKD protocol to further enhance the security of SNS-QKD. Numerical simulation results show that the protocol not only improves the security in source, but also retains the advantages of tolerating large misalignment errors. Therefore, it may provide further guidance for the practical application of SNS-QKD.
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http://dx.doi.org/10.3390/e24050662DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9140436PMC
May 2022

Organizational Emotional Capability Perspective: Research on the Impact of Psychological Capital on Enterprise Safety Performance.

Front Psychol 2022 20;13:854620. Epub 2022 Apr 20.

OCT Yunnan Cultural Investment Group Co., Ltd., Kunming, China.

Theoretical researchers of manager psychology have excellent potential to extend its research framework to more enterprise application areas, such as innovation, performance, and safety in production. Research in these areas has also been increasing in the past 10 years. Psychological capital is composed of four aspects: self-efficacy, hope, optimism, and tenacity. It plays an essential role in stimulating organizational growth and improving organizational performance. In safety management work, managers, as the core members of the organization, have a relationship between their psychological capital and employees' safety performance. Nevertheless, the closeness of the relationship between psychological capital and employee safety performance has not been fully demonstrated by academic circles. Based on positive psychology theory, this paper conducts a questionnaire survey of 157 managers and 314 employees related to safety work in manufacturing enterprises. From the new perspective of organizational emotional capability, this paper investigates the complex and extensive social-psychological role in organizations and combs, analyzes, and integrates relevant psychological research to construct the influence mechanism of managers' psychological capital and employee safety performance. Finally, the three important issues found based on data analysis were: (1) Managers' psychological capital has a significant positive impact both on employee safety performance and organizational emotional capability; (2) Organizational emotional capability has a significant positive impact on employee safety performance; (3) organizational emotional capability plays a partial mediating role in the relationship between managers' psychological capital and employee safety performance.
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http://dx.doi.org/10.3389/fpsyg.2022.854620DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9067434PMC
April 2022

Upregulation of Periostin Through CREB Participates in Myocardial Infarction-induced Myocardial Fibrosis.

J Cardiovasc Pharmacol 2022 May 1;79(5):687-697. Epub 2022 May 1.

Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Capital Medical University, Beijing, China.

Abstract: Myocardial fibrosis after myocardial infarction (MI) leads to heart failure, which has become an important global public health issue. One of the most important features of myocardial fibrosis is the abnormal deposition of extracellular matrix (ECM) proteins. Periostin is one of the ECM proteins. Cyclic AMP response element-binding protein 1 (CREB) is well known for its involvement in multiple signaling in myocardial fibrosis. It has been confirmed that CREB could regulate ECM proteins deposition. However, little is known about the relationship between CREB and periostin post-MI. This study aims to verify the hypothesis that CREB promotes the expression of periostin in MI-induced myocardial fibrosis. To test this hypothesis, primary rat cardiac fibroblasts were cultured and rat model of MI was established. The level of myocardial fibrosis post-MI was identified by histological staining. The expressions of CREB and periostin were detected through western blot and reverse transcription quantity polymerase chain reaction. The upregulation and downregulation of CREB and periostin were established by plasmid, small interfere RNA (siRNA), and lentivirus, respectively. High levels of CREB and periostin were found post-MI in our study. Meanwhile, the expression of periostin was decreased after CREB downregulation both in vivo and in vitro. Finally, with the treatment of pAV-CREB and si-periostin, the expressions of collagen Ⅰ and Ⅲ were attenuated. The expression of periostin was elevated post-MI and participated in MI-induced myocardial fibrosis, which was regulated through CREB. This study provides a novel idea and potential intervention target for MI-induced myocardial fibrosis.
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http://dx.doi.org/10.1097/FJC.0000000000001244DOI Listing
May 2022

Bridging D-A type photosensitizers with the azo group to boost intersystem crossing for efficient photodynamic therapy.

Chem Sci 2022 Apr 14;13(14):4139-4149. Epub 2022 Mar 14.

Key Laboratory of Functional Polymer Materials of Ministry of Education, State Key Laboratory of Medicinal Chemical Biology, Institute of Polymer Chemistry, College of Chemistry, Nankai University Tianjin 300071 China

Photodynamic therapy (PDT) has attracted much attention in disease treatments. However, the exploration of a novel method for the construction of outstanding photosensitizers (PSs) with stimuli-responsiveness remains challenging. In this study, we, for the first time, report a novel and effective strategy to boost reactive oxygen species (ROS) generation by bridging donor-acceptor (D-A) type PSs with the azo group. In contrast to the counterpart without azo-bridging, the azo-bridged PSs exhibit remarkably enhanced ROS generation both type-I and type-II photochemical reactions. Theoretical calculations suggest that azo-bridging leads to a prominent reduction in Δ , thereby enabling enhanced ROS generation efficient intersystem crossing (ISC). The resulting azo-bridged PS (denoted as Azo-TPA-Th(+)) exhibits a particularly strong bactericidal effect against clinically relevant drug-resistant bacteria, with the killing efficiency up to 99.999999% upon white light irradiation. Since azo-bridging generates an azobenzene structure, Azo-TPA-Th(+) can undergo -to- isomerization upon UV irradiation to form emissive aggregates by shutting down the ISC channel. By virtue of the fluorescence turn-on property of unbound Azo-TPA-Th(+), we propose a straightforward method to directly discern the effective photodynamic bactericidal dose without performing the tedious plate-counting assay. This study opens a brand-new avenue for the design of advanced PSs with both strong ROS generation and stimuli-responsiveness, holding great potential in high-quality PDT with rapid prediction of the therapeutic outcome.
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http://dx.doi.org/10.1039/d2sc00381cDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8985587PMC
April 2022

45S5 Bioglass® works synergistically with siRNA to downregulate the expression of matrix metalloproteinase-9 in diabetic wounds.

Acta Biomater 2022 06 12;145:372-389. Epub 2022 Apr 12.

School of Biomedical Engineering, Med-X Research Institute, Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai 200030, China; Chemical and Environment Engineering Department, School of Engineering, RMIT University, 124 La Trobe Street, Melbourne, VIC 3001, Australia. Electronic address:

Diabetic chronic wounds are difficult to heal because of the presence of excessive inflammation and high overexpression of matrix metalloproteinase-9 (MMP-9), which greatly affects the quality of life of patients with diabetes and increases the risk of death. Thus, the regulation of excessive inflammation and inhibition of MMP-9 overexpression are effective strategies to improve diabetic wound healing. The present study is the first to demonstrate that ion products of 45S5 Bioglass® (BG) can work with small interfering RNA of MMP9 (MMP9-siRNA) to reduce MMP-9 expression in tissue-forming cells and enhance the synthesis of extracellular matrix proteins (ECMs). Specifically, the BG ionic products can stimulate macrophages to convert to M2 phenotype, thereby creating a proregenerative inflammation microenvironment to indirectly suppress the expression of MMP-9 in tissue-forming cells. Chitosan nanoparticles encapsulating MMP9-siRNA (MMP9-siNP) can directly lower MMP-9 expression in tissue-forming cells. In addition, BG ionic products can promote the vascularization of endothelial cells and ECM protein synthesis by fibroblasts. Thus, injectable BG/sodium alginate (BG/SA) hydrogels loaded with MMP9-siNP can significantly accelerate the healing process of full-thickness excision wounds of diabetic rats by decreasing MMP-9 expression, improving collagen synthesis, and enhancing angiogenesis in the wounds, thereby demonstrating their great application potential in treating diabetic chronic wounds. STATEMENT OF SIGNIFICANCE: Excessive inflammation and high overexpression of MMP-9 have been considered as factors that severely hinder the healing process of diabetic chronic wounds. Effective strategies are required for the regulation of excessive inflammation and inhibition of MMP-9 overexpression to enhance diabetic wound healing. In the present work, an injectable bioglass/sodium alginate (BG/SA) hydrogel loaded with MMP9-siNP was developed; this hydrogel significantly accelerated the healing process of full-thickness excision wounds of diabetic rats by decreasing MMP-9 expression, improving collagen accumulation, and enhancing angiogenesis in the wounds. Thus, the BG/SA hydrogel loaded with MMP9-siNP has great potential for use in healing of diabetic chronic wounds.
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http://dx.doi.org/10.1016/j.actbio.2022.04.010DOI Listing
June 2022

Case Report: Concurrence of Dermatomyositis and Autoimmune Blistering Diseases: Two Case Reports and a Literature Review.

Front Immunol 2022 24;13:855408. Epub 2022 Mar 24.

Department of Dermatology, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

Dermatomyositis (DM) is an idiopathic inflammatory myopathy primarily involving skin and muscles. Clinically amyopathic dermatomyositis (CADM), a subset of DM, presents with characteristic cutaneous manifestations without clinical evidence of myositis. Although rare, vesiculobullous eruptions could develop in DM patients. Such "bullous DM" is commonly considered a sign of internal malignancy. However, some cases with similar presentations were diagnosed as autoimmune blistering disease eventually. Herein, we reported two cases of CADM with autoimmune blisters formed. Case 1 presented with vesicles and was diagnosed with CADM initially. However, this patient developed blisters again years later and was diagnosed with "pemphigus foliaceous" (PF) accordingly. Case 2, with a history of nasopharyngeal carcinoma and CADM, developed bullous pemphigoid several days after using a heat patch on her abdomen. The association between disease occurrence and local skin damage might provide more evidence to support the "epitope spreading" hypothesis. Moreover, we reviewed related literature and discussed the differences between the two disease entities in clinical presentations, pathogenesis, therapy, and the risk of complications.
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http://dx.doi.org/10.3389/fimmu.2022.855408DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8988126PMC
March 2022

Bioinspired -Derived wound dressings for localized drug-elution.

Bioact Mater 2022 Sep 14;15:482-494. Epub 2022 Jan 14.

Chongqing Key Laboratory of Oral Disease and Biomedical Sciences and Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education and Stomatological Hospital, Chongqing Medical University, Chongqing, 401174, China.

Local drug delivery has received increasing attention in recent years. However, the therapeutic efficacy of local delivery of drugs is still limited under certain scenarios, such as in the oral cavity or in wound beds after resection of tumors. In this study, we introduce a bioinspired adhesive hydrogel derived from the skin secretions of (SSAD) as a wound dressing for localized drug elution. The hydrogel was loaded with aminoguanidine or doxorubicin, and its controlled drug release and healing-promoting properties were verified in a diabetic rat palatal mucosal defect model and a C57BL/6 mouse melanoma-bearing model, respectively. The results showed that SSAD hydrogels with different pore sizes could release drugs in a controllable manner and accelerate wound healing. Transcriptome analyses of the palatal mucosa suggested that SSAD could significantly upregulate pathways linked to cell adhesion and extracellular matrix deposition and had the ability to recruit keratinocyte stem cells to defect sites. Taken together, these findings indicate that property-controllable SSAD hydrogels could be a promising biofunctional wound dressing for local drug delivery and promotion of wound healing.
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http://dx.doi.org/10.1016/j.bioactmat.2021.11.030DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8965088PMC
September 2022

Juxtamembrane 2 mimic peptide competitively inhibits mitochondrial trafficking and activates ROS-mediated apoptosis pathway to exert anti-tumor effects.

Cell Death Dis 2022 Mar 24;13(3):264. Epub 2022 Mar 24.

School of Biomedical Engineering, Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai, 200030, China.

Our previous study demonstrates that a juxtamembrane 2 (JM2) mimic peptide can inhibit proliferation and induce apoptosis of tumor cells. However, the mechanism remains unclear. In this study, JM2 is found to suppress the growth of 4T1 breast tumors by inducing apoptosis and inhibiting the proliferation of 4T1 tumor cells. Further study indicates that JM2 can stimulate the mitochondria to gather near the microtubule-organizing center of tumor cells and subsequently induce ROS-induced ROS release responses, which results in mitochondrial dysfunction and mitochondria-mediated apoptosis. In addition, JM2 can arrest cell cycle in S phase by regulating the expression of cell cycle-related proteins and consequently inhibit proliferation of tumor cells. Then, a previously designed JM2 grafted hyaluronic acid (HA) injectable hydrogel system (HA-JM2) is injected in a breast tumor-resected model and the HA-JM2 hydrogel can inhibit the malignant proliferation of residual tumor cells and suppress the breast tumor recurrence. These findings not only confirm the application potentials of JM2 in anti-tumor therapy and tumor post-surgery treatments but also provide greater understanding on the mechanisms by which JM2 inhibits tumor growth.
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http://dx.doi.org/10.1038/s41419-022-04639-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8948362PMC
March 2022

CircFOXM1 acts as a ceRNA to upregulate SMAD2 and promote the progression of nasopharyngeal carcinoma.

Mol Genet Genomic Med 2022 05 10;10(5):e1914. Epub 2022 Mar 10.

Xuzhou Medical University, Xuzhou, Jiangsu, China.

Background: In recent years, the development of high-throughput sequencing technology has promoted the rapid development of circRNA-related research. Studies have found that circRNA plays a key role in a variety of tumors, but few people study the role of circRNA in nasopharyngeal carcinoma. Under comprehensive treatments, the 5-year survival rate can reach about 70%, but some patients still have distant metastases or recurrences after treatment. Therefore, it is very important to study the molecular mechanisms of the proliferation and invasion of nasopharyngeal carcinoma.

Methods: QRT-PCR was applied to detect the relative expression level of circFOXM1 in NPC and nasopharyngeal epithelial cell lines. We knocked down circFOXM1 and studied the influence of circFOXM1 on NPC cells. Nuclear and cytoplasmic RNA isolation experiments, fluorescence in situ hybridization (FISH), bioinformatics analysis, the dual-luciferase reporter experiment, Western Blot, and other experiments were conducted to verify the relationships among circFOXM1, miR-136-5p, and SMAD2. We collected clinical NPC samples to prove the effect of circFOXM1 on the prognosis and treatment of NPC.

Results: In this study, we found that circFOXM1 is highly expressed in nasopharyngeal carcinoma tissue cells compared with adjacent normal tissues and is related to the staging of nasopharyngeal carcinoma. High expression of circFOXM1 indicates a poor prognosis for nasopharyngeal carcinoma. Knockdown of CircFOXM1 inhibited the proliferation and invasion of nasopharyngeal carcinoma cells.

Conclusion: CircFOXM1 promotes the malignant proliferation of nasopharyngeal carcinoma cells by regulating the miR-136-5p-SMAD2 axis.
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http://dx.doi.org/10.1002/mgg3.1914DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9034685PMC
May 2022

Three-dimensional static-fluid MR urography with gradient- and spin-echo (GRASE) at 3.0T: comparison of image quality and diagnostic performance with respiratory-triggered fast spin-echo (FSE).

Abdom Radiol (NY) 2022 05 2;47(5):1828-1839. Epub 2022 Mar 2.

Department of Radiology, Peking University First Hospital, No.8, Xishiku Street, Xicheng District, Beijing, 100034, China.

Purpose: To compare the performance of 3D MRU based on a breath-hold gradient- and spin-echo (BH-GRASE) technique with conventional 3D respiratory-triggered FSE (RT-FSE) sequence in patients with urinary tract dilation.

Methods: We prospectively included 90 patients with urinary tract dilation who underwent both 3D BH-GRASE and RT-FSE MRU at 3T. The acquisition time of two MRU sequences was recorded. Three readers blinded to the protocols reviewed the image quality using a five-point scale and assessed the diagnostic performance related to urinary tract dilation. The relative contrast ratio (CR) between the urinary tract and adjacent area was measured quantitatively.

Results: Acquisition time was 14.8 s for BH-GRASE MRU and 213.6 ± 52.2 s for RT-FSE MRU. The qualitative image analysis demonstrated significant equivalence between the two MRU protocols. 3D BH-GRASE MRU better depicted bilateral renal calyces than RT-FSE MRU (p < 0.05). The CR values of the urinary tract were lower on BH-GRASE MRU compared with RT-FSE MRU (p < 0.05). There were excellent agreements in the assessment of urinary tract dilation between BH-GRASE and RT-FSE MRU, including the dilated degree, obstructive level, and obstructive imaging features (inter-sequence κ = 0.924-1).

Conclusion: 3D BH-GRASE MRU significantly decreased the acquisition time and achieved comparable image quality, urinary tract visualization, and diagnostic performance with conventional 3D RT-FSE MRU. Breath-hold 3D MRU with GRASE may provide a feasible evaluation of urinary tract dilation.
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http://dx.doi.org/10.1007/s00261-022-03418-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9038886PMC
May 2022

Deficiency of two-pore segment channel 2 contributes to systemic lupus erythematosus via regulation of apoptosis and cell cycle.

Chin Med J (Engl) 2022 Jan 12;135(4):447-455. Epub 2022 Jan 12.

Department of Dermatology, China-Japan Friendship Hospital, Beijing 100029, China.

Background: Systemic lupus erythematosus (SLE) is a complex autoimmune disease, and the mechanism of SLE is yet to be fully elucidated. The aim of this study was to explore the role of two-pore segment channel 2 (TPCN2) in SLE pathogenesis.

Methods: Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to detect the expression of TPCN2 in SLE. We performed a loss-of-function assay by lentiviral construct in Jurkat and THP-1 cell. Knockdown of TPCN2 were confirmed at the RNA level by qRT-PCR and protein level by Western blotting. Cell Count Kit-8 and flow cytometry were used to analyze the cell proliferation, apoptosis, and cell cycle of TPCN2-deficient cells. In addition, gene expression profile of TPCN2-deficient cells was analyzed by RNA sequencing (RNA-seq).

Results: TPCN2 knockdown with short hairpin RNA (shRNA)-mediated lentiviruses inhibited cell proliferation, and induced apoptosis and cell-cycle arrest of G2/M phase in both Jurkat and THP-1 cells. We analyzed the transcriptome of knockdown-TPCN2-Jurkat cells, and screened the differential genes, which were enriched for the G2/M checkpoint, complement, and interleukin-6-Janus kinase-signal transducer and activator of transcription pathways, as well as changes in levels of forkhead box O, phosphatidylinositol 3-kinase/protein kinase B/mechanistic target of rapamycin, and T cell receptor pathways; moreover, TPCN2 significantly influenced cellular processes and biological regulation.

Conclusion: TPCN2 might be a potential protective factor against SLE.
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http://dx.doi.org/10.1097/CM9.0000000000001893DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8869567PMC
January 2022

Neutrophils in neutrophilic dermatoses: Emerging roles and promising targeted therapies.

J Allergy Clin Immunol 2022 04 18;149(4):1203-1205. Epub 2022 Feb 18.

Department of Dermatology, Xijing Hospital, Fourth Military Medical University, Xi'an, China. Electronic address:

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http://dx.doi.org/10.1016/j.jaci.2022.02.008DOI Listing
April 2022

Triptolide Suppresses NF-κB-Mediated Inflammatory Responses and Activates Expression of Nrf2-Mediated Antioxidant Genes to Alleviate Caerulein-Induced Acute Pancreatitis.

Int J Mol Sci 2022 Jan 23;23(3). Epub 2022 Jan 23.

School of Pharmaceutical Sciences, Jiangnan University, Wuxi 214122, China.

Triptolide (TP), the main active ingredient of , displays potent anti-inflammatory, antioxidant, and antiproliferative activities. In the present study, the effect of TP on acute pancreatitis and the underlying mechanisms of the disease were investigated using a caerulein-induced animal model of acute pancreatitis (AP) and an in vitro cell model. In vivo, pretreatment with TP notably ameliorated pancreatic damage, shown as the improvement in serum amylase and lipase levels and pancreatic morphology. Meanwhile, TP modulated the infiltration of neutrophils and macrophages (Ly6G staining and CD68 staining) and decreased the levels of proinflammatory factors (TNF-α and IL-6) through inhibiting the transactivation of nuclear factor-κB (NF-κB) in caerulein-treated mice. Furthermore, TP reverted changes in oxidative stress markers, including pancreatic glutathione (GSH), superoxide dismutase (SOD), and malondialdehyde (MDA), in acute pancreatitis mice. Additionally, TP pretreatment inhibited intracellular reactive oxygen species (ROS) levels via upregulated nuclear factor erythroid 2-related factor 2 (Nrf2) expression and Nrf2-regulated redox genes expression (HO-1, SOD1, GPx1 and NQO1) in vitro. Taken together, our data suggest that TP exert protection against pancreatic inflammation and tissue damage by inhibiting NF-κB transactivation, modulating immune cell responses and activating the Nrf2-mediated antioxidative system, thereby alleviating acute pancreatitis.
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http://dx.doi.org/10.3390/ijms23031252DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8835869PMC
January 2022

Timely and Appropriate Administration of Inhaled Argon Provides Better Outcomes for tMCAO Mice: A Controlled, Randomized, and Double-Blind Animal Study.

Neurocrit Care 2022 08 8;37(1):91-101. Epub 2022 Feb 8.

Stroke Center and Department of Neurology, Affiliated Hospital of Nantong University, Nantong, 226019, Jiangsu, China.

Background: Inhaled argon (iAr) has shown promising therapeutic efficacy for acute ischemic stroke and has exhibited impressive advantages over other inert gases as a neuroprotective agent. However, the optimal dose, duration, and time point of iAr for acute ischemic stroke are unknown. Here, we explored variable iAr schedules and evaluated the neuroprotective effects of acute iAr administration on lesion volume, brain edema, and neurological function in a mouse model of cerebral ischemic/reperfusion injury.

Methods: Adult ICR (Institute of Cancer Research) mice were randomly subjected to sham, moderate (1.5 h), or severe (3 h) transient middle cerebral artery occlusion (tMCAO). One hour after tMCAO, the mice were randomized to variable iAr protocols or air. General and focal deficit scores were assessed during double-blind treatment. Infarct volume, overall recovery, and brain edema were analyzed 24 h after cerebral ischemic/reperfusion injury.

Results: Compared with those in the tMCAO-only group, lesion volume (p < 0.0001) and neurologic outcome (general, p < 0.0001; focal, p < 0.0001) were significantly improved in the group administered iAr 1 h after stroke onset (during ischemia). Short-term argon treatment (1 or 3 h) significantly improved the infarct volume (1 vs. 24 h, p < 0.0001; 3 vs. 24 h, p < 0.0001) compared with argon inhalation for 24 h. The concentration of iAr was confirmed to be a key factor in improving focal neurological outcomes relative to that in the tMCAO group, with higher concentrations of iAr showing better effects. Additionally, even though ischemia research has shown an increase in cerebral damage proportional to the ischemia time, argon administration showed significant neuroprotective effects on infarct volume (p < 0.0001), neurological deficits (general, p < 0.0001; focal, p < 0.0001), weight recovery (p < 0.0001), and edema (p < 0.0001) in general, particularly in moderate stroke.

Conclusions: Timely iAr administration during ischemia showed optimal neurological outcomes and minimal infarct volumes. Moreover, an appropriate duration of argon administration was important for better neuroprotective efficacy. These findings may provide vital guidance for using argon as a neuroprotective agent and moving to clinical trials in acute ischemic stroke.
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http://dx.doi.org/10.1007/s12028-022-01448-9DOI Listing
August 2022

Polarity-Sensitive Fluorescent Probe for Reflecting the Packing Degree of Bacterial Membrane Lipids.

Anal Chem 2022 02 8;94(7):3303-3312. Epub 2022 Feb 8.

Key Laboratory of Functional Polymer Materials of Ministry of Education, State Key Laboratory of Medicinal Chemical Biology, Institute of Polymer Chemistry, College of Chemistry, Nankai University, Tianjin 300071, China.

The maintenance of an intact membrane structure is of great importance for bacteria to execute various biological functions. However, chemical probes for monitoring the dynamic changes of bacterial membranes are barely reported. Herein, we, for the first time, report a novel polarity-sensitive probe for reflecting the packing degree of bacterial membrane lipids. Specifically, we synthesize a membrane-targeting fluorescent probe (TICT-lipid) that possesses both twist intramolecular charge transfer and aggregation-induced emission properties. TICT-lipid exhibits sensitive responses to the minute difference in the packing degree of membrane lipids, facilitating rapid differentiation of Gram-negative and Gram-positive bacteria. Interestingly, in the presence of membrane-disrupting antibiotics, the localization of TICT-lipid shifts from the outer membrane to the cell membrane by outputting blue-shifted and enhanced emission, making the mechanism of action of antibiotics clearly visible. TICT-lipid is a polarity-sensitive fluorescent probe, holding great promise in the study of membrane-related bacterial processes and antibiotic screening.
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http://dx.doi.org/10.1021/acs.analchem.1c05268DOI Listing
February 2022

LCN2 Mediates Skin Inflammation in Psoriasis through the SREBP2‒NLRC4 Axis.

J Invest Dermatol 2022 Aug 2;142(8):2194-2204.e11. Epub 2022 Feb 2.

Department of Dermatology, Xijing Hospital, Fourth Military Medical University, Xi'an, China. Electronic address:

Lipocalins are a family of secreted adipokines that regulate cell lipid metabolism and immune responses. Although we have previously revealed that LCN2 modulates neutrophil activation in psoriasis, the other roles of LCN2 in psoriatic local inflammation have remained elusive. In this study, we found that 24p3R, the well-known specific receptor of LCN2, was highly expressed in the lesional epidermis of patients with psoriasis. Silencing 24p3R (also known as slc22a17) alleviated hyperkeratosis, inflammatory cell infiltration, and overexpression of inflammatory mediators in an imiquimod-induced psoriasis-like mouse model. In vitro, LCN2 enhanced the expression of proinflammatory factors in primary keratinocytes, such as IL-1β, IL-23, CXCL1, and CXCL10, which was paralleled by enforced cholesterol biosynthetic signaling. Importantly, taking in vivo and in vitro approaches, we discovered the SREBP2, a vital transcriptional factor in cholesterol synthesis pathway, as the critical mediator of LCN2-induced keratinocyte activation, which bound to the promoter region of NLRC4. Suppressing SREBP2 in mice attenuated NLRC4 signaling and psoriasis-like dermatitis. Taken together, this study identifies the critical role of LCN2‒SREBP2‒NLRC4 axis in the pathogenesis of psoriasis and proposes 24p3R or SREBP2 as a potential therapeutic target for psoriasis.
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http://dx.doi.org/10.1016/j.jid.2022.01.012DOI Listing
August 2022

Grading of clear cell renal cell carcinoma using diffusion MRI with a fractional order calculus model.

Acta Radiol 2022 Jan 18:2841851211072482. Epub 2022 Jan 18.

Department of Medical Imaging, Clinic Medical School, Yangzhou University, Northern Jiangsu Province Hospital, Yangzhou, PR China.

Background: The fractional order calculus (FROC) model has been developed to describe restrained motion of water molecules as well as microstructural heterogeneity, providing a novel tool for non-invasive tumor grading.

Purpose: To evaluate the role of the FROC model in characterizing clear cell renal cell carcinoma (ccRCC) grades.

Material And Methods: A total of 59 patients diagnosed with ccRCC were included in this prospective study. The diffusion metrics derived from the mono-exponential model (apparent diffusion coefficient [ADC]), intra-voxel incoherent motion [IVIM] model [D, D*, f], and FROC model [D, β, μ]) were calculated and compared between low- and high-grade ccRCCs. Binary logistic regression analysis was performed to establish the diagnostic models. Receiver operating characteristic (ROC) analysis and DeLong test were performed to evaluate and compare the diagnostic performance of metrics in grading ccRCC.

Results: All the metrics except D* and f exhibited statistical differences between low- and high-grade ccRCCs. ROC analysis showed individual FROC parameters, μ, D, and β, outperformed ADC and IVIM parameters in grading ccRCC. For single parameter, μ demonstrated the highest AUC value, sensitivity, and diagnostic accuracy in discriminating the two ccRCC groups while β exhibited the optimal specificity. Importantly, the combination of D, μ, and β could further improve the diagnostic performance.

Conclusion: The FROC parameters were superior to ADC and IVIM parameters in grading ccRCC, indicating the great potential of the FROC model in distinguishing low- and high-grade ccRCCs.
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http://dx.doi.org/10.1177/02841851211072482DOI Listing
January 2022

Effect of tissue expansion on chondrocyte sheets in cartilage composite reconstruction.

Am J Transl Res 2021 15;13(12):13438-13451. Epub 2021 Dec 15.

Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine 639 Zhi Zao Ju Road, Shanghai 200011, People's Republic of China.

Flap prelamination has been successfully established in tissue engineering; however, cartilage generation through combination of an expanded flap and chondrocyte sheets has not been reported. Herein, we investigate the effect of tissue expansion on chondrocyte sheets in prelaminating an expanded chondrocutaneous flap. Chondrocyte sheets were implanted into a tissue expander capsule following which capsule inflation was performed weekly. At 4 and 12 weeks post implantation, the specimens were examined with histology and immunohistochemistry analyses. Extracellular matrix (ECM) formation and type II collagen deposition in the regenerated cartilage tissue were also examined. After 4 weeks of implantation, the generated cartilage was phenotypically stable with minimal hypertrophy, while that formed after the 12-week expansion showed visible hypertrophic differentiation. To evaluate the effect of static pressure and/or hypoxic conditions generated by the expanding tissue, static pressure and/or hypoxic conditions were reproduced . The chondrocyte sheets stimulated by mechanical static pressure and hypoxia maintained their chondrogenic phenotype. The expression of aggrecan, collagen II, Sox-9, and HIF-1α was increased in chondrocyte sheets cultured in 2% oxygen (hypoxia); however, aggrecan, collagen II, and Sox-9 were downregulated in the static pressure/normoxia group. These results suggest that the expanded environment promoted cartilage formation by the chondrocyte cell sheets, while mechanical forces and hypoxic conditions allowed chondrocyte cell sheets to retain their chondrogenic phenotype.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8748122PMC
December 2021

Recurrence Risk of Liver Cancer Post-hepatectomy Using Machine Learning and Study of Correlation With Immune Infiltration.

Front Genet 2021 8;12:733654. Epub 2021 Dec 8.

Department of Information and Electronic Engineering, Zhejiang University of Science and Technology, Hangzhou, China.

Postoperative recurrence of liver cancer is the main obstacle to improving the survival rate of patients with liver cancer. We established an mRNA-based model to predict the risk of recurrence after hepatectomy for liver cancer and explored the relationship between immune infiltration and the risk of recurrence after hepatectomy for liver cancer. We performed a series of bioinformatics analyses on the gene expression profiles of patients with liver cancer, and selected 18 mRNAs as biomarkers for predicting the risk of recurrence of liver cancer using a machine learning method. At the same time, we evaluated the immune infiltration of the samples and conducted a joint analysis of the recurrence risk of liver cancer and found that B cell, B cell naive, T cell CD4 memory resting, and T cell CD4 were significantly correlated with the risk of postoperative recurrence of liver cancer. These results are helpful for early detection, intervention, and the individualized treatment of patients with liver cancer after surgical resection, and help to reveal the potential mechanism of liver cancer recurrence.
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http://dx.doi.org/10.3389/fgene.2021.733654DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8692778PMC
December 2021

Correlation between L3 skeletal muscle index and prognosis of patients with stage IV gastric cancer.

J Gastrointest Oncol 2021 Oct;12(5):2073-2081

Department of Medical Oncology, Jiangsu Cancer Hospital and Jiangsu Institute of Cancer Prevention and The Affiliated Cancer Hospital of Nanjing Medical University, Nanjing, China.

Background: To explore the relationship between L3 skeletal muscle index (SMI) and the prognosis of patients with stage IV gastric cancer (GC).

Methods: A total of 27 patients with stage IV GC requiring chemotherapy admitted to our hospital from 1 April 2015 to 20 May 2019 were selected as participants. The Kaplan-Meier method was used to describe the survival time of all participants. By evaluating the L3 plane CT images, the mass index (cm/m) of L3 skeletal muscle (including psoas major, erector spinae, quadratus psoas, transversus abdominis, external oblique abdominis, and internal oblique abdominis) was calculated to study the changes of L3 SMI during treatment and the correlation between L3 SMI and clinical features. The log-rank method was used to analyze the correlativity between the survival time of patients and their general data, L3 SMI, or other indicators.

Results: The survival time of 27 patients with stage IV GC was 7.4-49.9 months, with a mean survival time of 19.72 months and a median survival time of 16.17 months. The 1-year survival rate was 77.78%, and the 3-year survival rate was 7.41%. During treatment, L3 SMI continued to decline in 20 of the 27 participants (74.07%). After the first chemotherapy, 17 participants (62.96%) met the criteria of sarcopenia syndrome, and after the fourth chemotherapy, 19 participants (70.37%) met the criteria of sarcopenia syndrome. The L3 SMI was shown to be significantly correlated with body mass index (BMI) and Onodera's prognostic nutritional index (OPNI) (both P<0.05), but not with age, gender, dietary intake, and primary site (all P>0.05). Log-rank test showed that there was a correlation between L3 SMI and survival time of patients (P<0.05). The average survival time of participants with sarcopenia syndrome (16.78 months) was significantly lower than that of those without sarcopenia syndrome (25.58 months) (P<0.05).

Conclusions: There is a significant correlation between L3 SMI and survival time, and L3 SMI can be used as a potential index to evaluate the prognosis of patients with stage IV GC.
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http://dx.doi.org/10.21037/jgo-21-556DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8576239PMC
October 2021

Adipocyte-Derived CTRP3 Exhibits Anti-Inflammatory Effects via LAMP1-STAT3 Axis in Psoriasis.

J Invest Dermatol 2022 05 21;142(5):1349-1359.e8. Epub 2021 Oct 21.

Department of Dermatology, Xijing Hospital, Fourth Military Medical University, Xi'an, China. Electronic address:

Psoriasis is a systemic disease that is associated with metabolic disorders, which may contribute to abnormal adipokine levels. However, the underlying mechanism is largely unknown. Here, we investigated the role of the adipokine CTRP3 in the pathogenesis of psoriasis and comorbidities. The circulating CTRP3 level in patients with psoriasis was significantly lower than that in healthy controls and negatively correlated with metabolic risk factors. Rescuing CTRP3 levels with the GLP-1 receptor agonist exendin-4 in diet-induced obese mice could alleviate its more severe psoriatic symptoms in an imiquimod-induced mouse model. Topical application of CTRP3 also exerted a protective effect on imiquimod-induced normal diet mice. Moreover, CTRP3 could directly inhibit the inflammatory responses of psoriatic keratinocytes by blocking phosphorylation of signal transducer and activator of transcription 3 via LAMP1 in vitro. We identified the critical psoriatic cytokines, including IL-17A and TNF-α, that impaired adipocyte differentiation and sufficient CTRP3 secretion. In sum, our study reveals that adipocyte dysfunction and low level of CTRP3 caused by IL-17A exacerbates psoriasis progression and related metabolic syndrome, implying a mechanism underlying the vicious cycle between psoriasis and metabolic disorders. Pharmacological agents that improve CTRP3 level in obese patients with psoriasis may be considered as a potential strategy for psoriasis treatment.
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http://dx.doi.org/10.1016/j.jid.2021.09.027DOI Listing
May 2022
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