Publications by authors named "Ke Li"

1,980 Publications

  • Page 1 of 1

Pyrroloquinoline quinone extends ' longevity through the insulin/IGF1 signaling pathway-mediated activation of autophagy.

Food Funct 2021 Oct 14. Epub 2021 Oct 14.

Department of Nutrition and Food Science, School of Public Health, Tianjin Medical University, Tianjin 300070, China.

Aging is the leading cause of human morbidity and death worldwide. Pyrroloquinoline quinone (PQQ) is a water-soluble vitamin-like compound that has strong anti-oxidant capacity. Beneficial effects of PQQ on lifespan have been discovered in the model organism (), yet the underlying mechanisms remain unclear. In the current study, we hypothesized that the longevity-extending effect of PQQ may be linked to autophagy and insulin/IGF1 signaling (IIS) in Our data demonstrate that PQQ at a concentration of 1 mM maximally extended the mean life of by 33.1%. PQQ increased locomotion and anti-stress ability, and reduced fat accumulation and reactive oxygen species (ROS) levels. There was no significant lifespan extension in PQQ-treated , , and mutants, suggesting that these IIS- and autophagy-related genes may mediate the anti-aging effects of the PQQ. Furthermore, PQQ raised mRNA expression and the nuclear localization of the pivotal transcription factor , and then activated its downstream targets , , and . Enhanced activity of the autophagy pathway was also observed in PQQ-fed , as evidenced by increased expression of the key autophagy genes including , and , and also by an increase in the GFP::LGG-1 puncta. Inactivation of the IIS pathway-related genes or by RNAi partially blocked the increase in autophagy activity caused by PQQ treatment, suggesting that autophagy may be regulated by IIS. This study demonstrates that anti-aging properties of PQQ, in the model, may be mediated the IIS pathway and autophagy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d1fo02128aDOI Listing
October 2021

Software Training in HEP.

Comput Softw Big Sci 2021 8;5(1):22. Epub 2021 Oct 8.

University of Michigan, Ann Arbor, MI USA.

The long-term sustainability of the high-energy physics (HEP) research software ecosystem is essential to the field. With new facilities and upgrades coming online throughout the 2020s, this will only become increasingly important. Meeting the sustainability challenge requires a workforce with a combination of HEP domain knowledge and advanced software skills. The required software skills fall into three broad groups. The first is fundamental and generic software engineering (e.g., Unix, version control, C++, and continuous integration). The second is knowledge of domain-specific HEP packages and practices (e.g., the ROOT data format and analysis framework). The third is more advanced knowledge involving specialized techniques, including parallel programming, machine learning and data science tools, and techniques to maintain software projects at all scales. This paper discusses the collective software training program in HEP led by the HEP Software Foundation (HSF) and the Institute for Research and Innovation in Software in HEP (IRIS-HEP). The program equips participants with an array of software skills that serve as ingredients for the solution of HEP computing challenges. Beyond serving the community by ensuring that members are able to pursue research goals, the program serves individuals by providing intellectual capital and transferable skills important to careers in the realm of software and computing, inside or outside HEP.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s41781-021-00069-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8497185PMC
October 2021

Economic and intensity effects of coal consumption in China.

J Environ Manage 2021 Oct 7;301:113912. Epub 2021 Oct 7.

School of Statistics, Southwestern University of Finance and Economics, Chengdu, Sichuan, 611130, PR China. Electronic address:

The increase in coal consumption and its impact on the environment has become a bottleneck that hinders sustainable development. This paper discusses the effect of economic growth and coal intensity on China's coal consumption during 2005-2017 using the Laspeyres index decomposition method. The decoupling of coal consumption from economic growth was examined in conjunction with the Tapio elasticity index, and the decoupling contributions of economic growth and coal intensity are further determined. The results indicated that economic growth drives an increase in coal consumption; however, the contribution rate declines gradually with decrease in economic growth rate in each province. Further research showed that the secondary industry is the main contributor to the increment, and the rapid development of tertiary industry increases indirect coal consumption. Coal intensity has a positive impact on curbing coal consumption, but it is not sufficient to offset the increment generated by the economic effect. Moreover, in each province, the curbing effect gradually decreased as the decline in coal intensity weakened in the secondary industry. Furthermore, coal consumption is weakly decoupled from economic growth over the long term, and the secondary industry will determine the future trend of decoupling.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jenvman.2021.113912DOI Listing
October 2021

Observation of D^{0}→K_{1}(1270)^{-}e^{+}ν_{e}.

Phys Rev Lett 2021 Sep;127(13):131801

Turkish Accelerator Center Particle Factory Group, Istanbul Bilgi University, 34060 Eyup, Istanbul, Turkey.

Using 2.93  fb^{-1} of e^{+}e^{-} collision data taken with the BESIII detector at a center-of-mass energy of 3.773 GeV, the observation of the D^{0}→K_{1}(1270)^{-}e^{+}ν_{e} semileptonic decay is presented. The statistical significance of the decay D^{0}→K_{1}(1270)^{-}e^{+}ν_{e} is greater than 10σ. The branching fraction of D^{0}→K_{1}(1270)^{-}e^{+}ν_{e} is measured to be (1.09±0.13_{-0.16}^{+0.09}±0.12)×10^{-3}. Here, the first uncertainty is statistical, the second is systematic, and the third originates from the assumed branching fraction of K_{1}(1270)^{-}→K^{-}π^{+}π^{-}. The fraction of longitudinal polarization in D^{0}→K_{1}(1270)^{-}e^{+}ν_{e} is determined for the first time to be 0.50±0.19_{stat}±0.08_{syst}.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1103/PhysRevLett.127.131801DOI Listing
September 2021

Ni-catalyzed hydroalkylation of olefins with N-sulfonyl amines.

Nat Commun 2021 Oct 7;12(1):5881. Epub 2021 Oct 7.

Key Laboratory of Precise Synthesis of Functional Molecules of Zhejiang Province, School of Science, Westlake University, 18 Shilongshan Road, Hangzhou, 310024, China.

Hydroalkylation, the direct addition of a C(sp)-H bond across an olefin, is a desirable strategy to produce valuable, complex structural motifs in functional materials, pharmaceuticals, and natural products. Herein, we report a reliable method for accessing α-branched amines via nickel-catalyzed hydroalkylation reactions. Specifically, by using bis(cyclooctadiene)nickel (Ni(cod)) together with a phosphine ligand, we achieved a formal C(sp)-H bond insertion reaction between olefins and N-sulfonyl amines without the need for an external hydride source. The amine not only provides the alkyl motif but also delivers hydride to the olefin by means of a nickel-engaged β-hydride elimination/reductive elimination process. This method provides a platform for constructing chiral α-branched amines by using a P-chiral ligand, demonstrating its potential utility in organic synthesis. Notably, a sulfonamidyl boronate complex formed in situ under basic conditions promotes ring-opening of the azanickellacycle reaction intermediate, leading to a significant improvement of the catalytic efficiency.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41467-021-26194-yDOI Listing
October 2021

Atlantoaxial Non-Fusion Using Biomimetic Artificial Atlanto-Odontoid Joint: Technical Innovation and Initial Biomechanical Study.

Spine (Phila Pa 1976) 2021 Oct 5. Epub 2021 Oct 5.

Department of Orthopaedics, Second Affiliated Hospital of Medical School of Xi'an Jiaotong University, Xi'an, Shaanxi, P.R. China Department of Physical Medicine and Rehabilitation, Zibo Central Hospital, Zibo, Shandong, PR. China Department of Ultrasound, Second Affiliated Hospital of Medical School of Xi'an Jiaotong University, Xi'an, Shaanxi, P.R. China Department of Orthopaedics, Xi'an International Medical Center Hospital, Xi'an, Shaanxi, P.R. China.

Study Design: A Biomechanical in vitro Investigation.

Objective: To evaluate the function and stability of self-designed biomimetic artificial atlanto-odontoid joint(BAAOJ) replacement on the atlantoaxial joint.

Summary Of Background Data: Upper cervical fusion surgery is a common treatment for various atlantoaxial disorders, and favorable clinical outcome has been achieved. However, the fusion surgery results in loss of atlantoaxial motion as well as adjacent segments degeneration, reducing the quality of life of patients and might produce severe neurological symptoms. Non-fusion technology is expected to solve the above problems, but various designed devices have certain defects and are still in the exploratory phase.

Methods: Biomechanical tests were conducted on 10 fresh human cadaveric craniocervical specimens in the following sequence:(1) intact condition, (2) after the BAAOJ arthroplasty, (3) after BAAOJ fatigue test, (4) after odontoidectomy, and (5) after anterior rigid plate fixation. Three-dimensional movements of the C1-C2 segment were evaluated to investigate the function and stability of BAAOJ arthroplasty compared with the intact condition after the BAAOJ fatigue test, odontoidectomy, and rigid plate fixation.

Results: Comparing the BAAOJ implantation to the intact state, the ROM and neutral zone (NZ) were slightly reduced in all directions (P>0.05). Compared with the rigid plate fixation, the BAAOJ implantation significantly increased the ROM and NZ in all directions, especially in the axial rotation (P<0.05).

Conclusion: We designed a BAAOJ for correcting atlantoaxial disorders arising from atlantoaxial instability. As a non-fusion device, the most critical feature of BAAOJ replacement is the retention of flexion-extension, lateral bending, and axial rotation range of motion similar to the normal state. It can also stabilize the atlantoaxial complex, and the BAAOJ itself has a good initial stability.Level of Evidence: 4.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/BRS.0000000000004259DOI Listing
October 2021

Pheromone pollution from invasive sea lamprey misguides a native confamilial.

Curr Zool 2021 Jun 21;67(3):333-335. Epub 2020 Oct 21.

Department of Fisheries and Wildlife, Michigan State University, East Lansing, MI 48825, USA.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1093/cz/zoaa064DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8488990PMC
June 2021

Ultra-broadband Bragg concave diffraction grating designs on 220-nm SOI for wavelength demultiplexing.

Opt Express 2021 Sep;29(19):30259-30271

The appropriate broadband design of a de/multiplexer can significantly increase the channel number and consequently the transmission capacity of a wavelength division multiplexing system. Herein, we present the first ultra-broadband Bragg concave diffraction grating (CDG) on a 220-nm silicon-on-insulator, covering most of the E, S, C, L, and U telecommunication wavebands spanning from 1.425 to 1.675 μm. A wide-band-gap Bragg mirror is employed to facilitate broadband reflection, with a low diffraction order of grating for a sufficient free spectral range. Numerical simulations show that the proposed approaching blazed concave diffraction grating (AB-CDG) for the two-material case exhibits a high integration, simple fabrication process, and promising spectral performance. We fabricate the grating for design verification with a low transmission loss of -0.6 dB and a crosstalk below -33.7 dB for the eight measured wavelength channels covering the spectral range from 1.5 to 1.61 μm that is limited by the bandwidth of the grating coupler. This design can be used for broadband wavelength demultiplexing, frontier astronomical observation, and spectroscopic imaging.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1364/OE.431133DOI Listing
September 2021

Optical OAM tweezer based on graded-index multimode fibers.

Appl Opt 2021 Sep;60(25):7634-7639

A fiber-optic probe consisting of a section of graded-index multimode fiber (GIMF) fused onto a few-mode fiber (FMF) is proposed in this paper. The orbital angular momentum (OAM) mode guided by the FMF was launched into the GIMF, and a focused OAM beam profile was obtained by tailoring the length of the GIMF. Based on the analysis of the propagation trajectory, the intensity distributions, and the phase distributions of the vortex beam in GIMF, the focusing properties of the OAM mode were investigated. It is found that there exists a maximum working distance at an optimal GIMF length, and a trade-off between the beam size and working distance should be taken into account for optical tweezer applications. These results are expected to be applied to optical fiber tweezers for more flexible and efficient optical manipulation of particles.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1364/AO.431057DOI Listing
September 2021

LncRNA TUG1 contributes to the tumorigenesis of lung adenocarcinoma by regulating miR-138-5p-HIF1A axis.

Int J Immunopathol Pharmacol 2021 Jan-Dec;35:20587384211048265

Department of Palliative Medicine, 531840Yunnan Cancer Hospital, Kunming, Yunnan, China.

Introduction: Increasing evidence indicates that lncRNA TUG1 represents an oncogenic factor in cancer. But, the mechanisms by which lncRNA TUG1 contributes to lung adenocarcinoma (LAC) remain undocumented.

Methods: The relationship between lncRNA TUG1/miR-138-5p expression and clinical outcomes in patients with LAC was indicated by qPCR, FISH, and TCGA cohort. Gain- or loss-of-function experiments and tumorigenesis were used to assess the role of lncRNA TUG1 in LAC. The interplay between TUG1 and miR-138-5p was validated by luciferase gene report and RIP assays. qPCR and Western blot analyses were used to investigate the effects of TUG1 on miR-138-5p/HIF1A axis in LAC cells.

Results: We found that upregulation of TUG1 or downregulation of miR-138-5p was associated with lymph node or distant metastasis and indicated a poor survival in LAC. Reduced expression of TUG1 restrained the growth of LAC cells, while restored expression of TUG1 had the opposite effects. TUG1 was identified to negatively regulate miR-138-5p expression, and miR-138-5p reversed TUG1-induced cell proliferation by targeting HIF1A. Elevated expression of HIF1A predicted a poor survival in LAC.

Conclusion: Our findings demonstrate that lncRNA TUG1 promotes the growth of LAC by regulating miR-138-5p-HIF1A axis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1177/20587384211048265DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8495526PMC
October 2021

Multiplex Recurrence Network Analysis of Inter-Muscular Coordination During Sustained Grip and Pinch Contractions at Different Force Levels.

IEEE Trans Neural Syst Rehabil Eng 2021 13;29:2055-2066. Epub 2021 Oct 13.

Production of functional forces by human motor systems require coordination across multiple muscles. Grip and pinch are two prototypes for grasping force production. Each grasp plays a role in a range of hand functions and can provide an excellent paradigm for studying fine motor control. Despite previous investigations that have characterized muscle synergies during general force production, it is still unclear how intermuscular coordination differs between grip and pinch and across different force outputs. Traditional muscle synergy analyses, such as non-negative matrix factorization or principal component analysis, utilize dimensional reduction without consideration of nonlinear characteristics of muscle co-activations. In this study, we investigated the novel method of multiplex recurrence networks (MRN) to assess the inter-muscular coordination for both grip and pinch at different force levels. Unlike traditional methods, the MRN can leverage intrinsic similarities in muscle contraction dynamics and project its layers to the corresponding weighted network (WN) to better model muscle interactions. Twenty-four healthy volunteers were instructed to grip and pinch an apparatus with force production at 30%, 50%, and 70% of their respective maximal voluntary contraction (MVC). The surface electromyography (sEMG) signals were recorded from eight muscles, including intrinsic and extrinsic muscles spanning the hand and forearm. The sEMG signals were then analyzed using MRNs and WNs. Interlayer mutual information ( I ) and average edge overlap ( ω ) of MRNs and average shortest path length ( L ) of WNs were computed and compared across groups for grasp types (grip vs. pinch) and force levels (30%, 50% and 70% MVC). Results showed that the extrinsic, rather than the intrinsic muscles, had significant differences in network parameters between both grasp types ( ), and force levels ( ), and especially at higher force levels. Furthermore, I and ω were strengthened over time ( ) except with pinch at 30% MVC. Results suggest that the central nervous system (CNS) actively increases cortical oscillations over time in response to increasing force levels and changes in force production with different sustained grasping types. Muscle coupling in extrinsic muscles was higher than in intrinsic muscles for both grip and pinch. The MRNs may be a valuable tool to provide greater insights into inter-muscular coordination patterns of clinical populations, assess neuromuscular function, or stabilize force control in prosthetic hands.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1109/TNSRE.2021.3117286DOI Listing
October 2021

Cascade Release Nanocarriers for the Triple-Negative Breast Cancer Near-Infrared Imaging and Photothermal-Chemo Synergistic Therapy.

Front Oncol 2021 15;11:747608. Epub 2021 Sep 15.

College of Clinical Medicine, Xi'an Medical University, Xi'an, China.

Triple-negative breast cancer (TNBC) has inadequate treatment approaches and a poor prognosis. It is urgent to develop new treatment approaches for TNBC. The combination of photothermal therapy (PTT) and chemotherapy is a very effective potential therapy for TNBC. However, asynchronous accumulation, unclear efficacy, and toxic side effects hinder the further promotion of this method. Therefore, we designed and constructed a new type of nanocarriers, the cascade release near-infrared imaging (NIFI) & thermal-chemo combination nanoparticles (CNC NPs), that can release drugs through the cascade of ultrasound triggering and pH responding to achieve the synchronous tumor accumulation, monitoring and synergistic treatment of two functional molecules. The key material of CNC NPs is the polydopamine (PDA), which, through self-assembling, forms a rigid shell that contains doxorubicin (DOX) and NIF fluorescent dye IR780 on the surface of the perfluorohexane (PFH) microbubbles. The results show that CNC NPs have a hollow core-shell structure with an average particle size of 97.3 ± 27.2 nm and have exceptional colloidal stability and photothermal conversion efficiency. The NPs can effectively perform cascade drug release through ultrasound triggering and pH responding. CNC NPs have good biological safety and excellent fluorescence imaging, drug delivery, and therapeutic abilities in the TNBC models. These results provide an experimental basis for the development of new clinical treatment methods for TNBC.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fonc.2021.747608DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8480474PMC
September 2021

Control of Newly-Designed Wearable Robotic Hand Exoskeleton Based on Surface Electromyographic Signals.

Front Neurorobot 2021 15;15:711047. Epub 2021 Sep 15.

Department of Geriatrics, Qilu Hospital, Shandong University, Jinan, China.

The human hand plays a role in a variety of daily activities. This intricate instrument is vulnerable to trauma or neuromuscular disorders. Wearable robotic exoskeletons are an advanced technology with the potential to remarkably promote the recovery of hand function. However, the still face persistent challenges in mechanical and functional integration, with real-time control of the multiactuators in accordance with the motion intentions of the user being a particular sticking point. In this study, we demonstrated a newly-designed wearable robotic hand exoskeleton with multijoints, more degrees of freedom (DOFs), and a larger range of motion (ROM). The exoskeleton hand comprises six linear actuators (two for the thumb and the other four for the fingers) and can realize both independent movements of each digit and coordinative movement involving multiple fingers for grasp and pinch. The kinematic parameters of the hand exoskeleton were analyzed by a motion capture system. The exoskeleton showed higher ROM of the proximal interphalangeal and distal interphalangeal joints compared with the other exoskeletons. Five classifiers including support vector machine (SVM), K-near neighbor (KNN), decision tree (DT), multilayer perceptron (MLP), and multichannel convolutional neural networks (multichannel CNN) were compared for the offline classification. The SVM and KNN had a higher accuracy than the others, reaching up to 99%. For the online classification, three out of the five subjects showed an accuracy of about 80%, and one subject showed an accuracy over 90%. These results suggest that the new wearable exoskeleton could facilitate hand rehabilitation for a larger ROM and higher dexterity and could be controlled according to the motion intention of the subjects.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fnbot.2021.711047DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8480391PMC
September 2021

A multifunctional hydrogel coating to direct fibroblast activation and infected wound healing via simultaneously controllable photobiomodulation and photodynamic therapies.

Biomaterials 2021 Sep 28;278:121164. Epub 2021 Sep 28.

Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing, 400044, China; Chongqing Key Laboratory of Soft-Matter Material Chemistry and Function Manufacturing, Southwest University, Chongqing, 400715, China. Electronic address:

Bacterial infection treatment and subsequent tissue rebuilding are the main tasks of biomaterial research. To endow implants with antibacterial activity and biological functions, the material systems are usually very complicated and ineffective. Recently, the concept of photobiomodulation (PBM), or low-level laser therapy (LLLT), has attracted increasing attention in tissue repair applications but still has not obtained wide acceptance. Because of the same laser resource, PBM could simultaneously work with 660 nm laser triggered photodynamic therapy (PDT), which will significantly simplify the material system and achieve the multiple functions of antibacterial activity and biological modulation effects. Herein, we attempt to validate the effectiveness of PBM and combine PBM with a PDT-based material system. A catechol motif-modified methacrylated gelatin containing photosensitizer Chlorin e6-loaded mesoporous polydopamine nanoparticles was fabricated (GelMAc/[email protected]). This hydrogel could be tightly adhered to titanium surfaces to serve as surface coating materials or directly used as dressings. Because of the 660 nm laser-triggered ROS generation property of Ce6, GelMAc/[email protected] exhibited a remarkable and rapid antibacterial activity when the laser power was 1 W cm. After bacterial elimination, when the power was adjusted to 100 mW cm, daily irradiation brought an excellent PBM effect: the fibroblast activation was realized to accelerate wound repair. According to our in vitro and in vivo results, the fabricated hydrogel coating possessed both antibacterial activity and fibroblast activation ability only by adjusting the power of laser irradiation, which will greatly strengthen the confidence of using PBM in broader fields and give a good example to combine PBM with traditional biomaterial design.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biomaterials.2021.121164DOI Listing
September 2021

First Measurement of the Absolute Branching Fraction of Λ→pμ^{-}ν[over ¯]_{μ}.

Phys Rev Lett 2021 Sep;127(12):121802

Turkish Accelerator Center Particle Factory Group, Istinye University, 34010 Istanbul, Turkey.

The absolute branching fraction of Λ→pμ^{-}ν[over ¯]_{μ} is reported for the first time based on an e^{+}e^{-} annihilation sample of 10×10^{9}  J/ψ events collected with the BESIII detector at sqrt[s]=3.097  GeV. The branching fraction is determined to be B(Λ→pμ^{-}ν[over ¯]_{μ})=[1.48±0.21(stat)±0.08(syst)]×10^{-4}, which is improved by about 30% in precision over the previous indirect measurements. Combining this result with the world average of B(Λ→pe^{-}ν[over ¯]_{e}), we obtain the ratio {[Γ(Λ→pμ^{-}ν[over ¯]_{μ})]/[Γ(Λ→pe^{-}ν[over ¯]_{e})]} to be 0.178±0.028, which agrees with the standard model prediction assuming lepton flavor universality. The asymmetry of the branching fractions of Λ→pμ^{-}ν[over ¯]_{μ} and Λ[over ¯]→p[over ¯]μ^{+}ν_{μ} is also determined, and no evidence for CP violation is found.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1103/PhysRevLett.127.121802DOI Listing
September 2021

Characterization of the complete mitochondrial genome and phylogenetic status of a recently described species of Mountain Dragon, (Reptilia: Squamata: Agamidae), from the upper Lantsang valley in west China.

Mitochondrial DNA B Resour 2021 27;6(10):3041-3043. Epub 2021 Sep 27.

Museum of Herpetology, Chengdu Institute of Biology, Chengdu, China.

The mountain dragon, , is an endemic and protected valley lizard that inhabits the upper Lantsang Valley in West China. In this study, we sequenced the complete mitochondrial genome of a male individuals of using next-generation sequencing methodologies. The complete mitogenome is 16,432 bp in length and contains one noncoding control regions, 13 protein-coding, 22 transfer RNA and two ribosomal RNA genes. The mitogenome content and structure of D. vela was consistent with the previously published representatives of the family. A Bayesian phylogenetic analysis using the complete mitochondrial genomes of Agamidae fully resolved in the Draconinae, a result consistent with previous investigations. This study provides bioinformatic data for better understanding the evolution and the phylogenetic history of the mountain dragon.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/23802359.2021.1962756DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8477936PMC
September 2021

Relationship Between Blood Glucose Level and Prevalence and Frequency of Stress Urinary Incontinence in Women.

Female Pelvic Med Reconstr Surg 2021 Sep 27. Epub 2021 Sep 27.

From the Department of Endocrine Department of Nephrology Department of Urology, The First People's Hospital of Yongkang, Jinhua City, Zhejiang Province, China.

Objectives: The purpose of this study was to evaluate the relationship between blood glucose level and the prevalence and frequency of stress urinary incontinence (SUI) in women.

Methods: We conducted a cross-sectional study of female participants in the National Health and Nutrition Examination Survey database between 2007 and 2016. Dose-response analysis curves and univariate and multivariate logistic regressions were used to determine the relationship between blood glucose level and the prevalence and frequency of SUI.

Results: A total of 10,771 participants were included in this study, of which 6,466 (60.0%) reported no SUI, 4,305 (31.1%) reported monthly SUI, and 953 (8.8%) reported weekly SUI. We found that the blood glucose levels were higher in the weekly SUI group than in the monthly SUI and no SUI groups. Based on blood glucose levels, participants were divided into 3 groups: ≤86.0 mg/dL group, >86.0 to 98.0 mg/dL group, and >98.0 mg/dL group. Dose-response curves showed a nonlinear positive correlation between blood glucose levels and the prevalence and extent of SUI, and participants in the glucose >98.0 mg/dL group had a 15.2% higher risk (adjusted odds risk, 1.152; 95% confidence interval, 1.027-1.293; P = 0.016) of SUI prevalence and 12.5% higher risk (adjusted odds risk 1.125; 95% confidence interval, 1.009-1.255; P = 0.034) of SUI frequency than participants in the glucose ≤86.0 mg/dL group.

Conclusions: We found that the prevalence and frequency of SUI in women were positively correlated with blood glucose levels, and these findings warrant further study and application to clinical practice to control SUI in women.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/SPV.0000000000001112DOI Listing
September 2021

Protocol for chemically induced murine gastric tumor model.

STAR Protoc 2021 Dec 17;2(4):100814. Epub 2021 Sep 17.

Bio-X Institutes, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders, Ministry of Education, Shanghai Jiao Tong University, Shanghai 200240, China.

N-Methyl-N-nitrosourea, an N-nitroso compound converted from dietary nitrite by , causes somatic mutations in epithelial cells and induces gastric premalignancy. Here, we describe a detailed protocol for induction of gastric tumor and analysis of tumor phenotypes in mice. This model can be widely used for studying the initiation and growth of gastric cancer. For complete details on the use and execution of this protocol, please refer to Li et al. (2021).
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.xpro.2021.100814DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8456110PMC
December 2021

Molecular engineering of organic-based agents for bioimaging and phototherapeutics.

Chem Soc Rev 2021 Sep 27. Epub 2021 Sep 27.

Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan 410082, China.

monitoring of the location and transportation of bioactive molecules is essential for deciphering diverse biological events in the field of biomedicine. In addition, obtaining the information of lesions will provide a clear perspective for surgeons to perform precise resection in clinical surgery. Notably, delivering drugs or operating photodynamic therapy/photothermal therapy by labeling the lesion regions of interest can improve treatment and reduce side effects . In various advanced imaging and therapy modalities, optical theranostic agents based on organic small molecules can be conveniently modified as needed and can be easily internalized into cells/lesions in a non-invasive manner, which are prerequisites for bioimaging and precision treatment. In this tutorial review, we first summarize the molecular immobilization strategies to retain small-molecule agents inside cells/lesions to prevent their diffusion in living organisms. Emphasis will be focused on introducing the application of these strategies for imaging of biomolecules and precision treatment, particularly pertaining to why targeting therapy is required.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d1cs00408eDOI Listing
September 2021

Perioperative Bilateral Medial Medullary Infarction With "Snake Eyes Appearance": A Case Report.

Front Med (Lausanne) 2021 9;8:559381. Epub 2021 Sep 9.

Department of Neurology, Aerospace Center Hospital, Beijing, China.

Perioperative bilateral medial medullary infarction (BMMI) cases mimicking acute motor axonal neuropathy (AMAN) under general anesthesia have not been reported. We describe a patient who suffered flaccid quadriplegia and could not wean from mechanical ventilation after emergence from general anesthesia in cardiac surgery. A diagnosis of AMAN was considered, but intravenous immunoglobulin showed little efficacy. Magnetic resonance imaging of the patient later revealed BMMI with "snake eyes appearance," and he was found to have severe vertebral artery stenosis. Considering the association between severe coronary heart disease and cerebrovascular stenosis, we highlight the significance of preoperative evaluation and comprehensive management of the cerebrovascular system for certain patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fmed.2021.559381DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8458652PMC
September 2021

[Co(μ-O)]-Based Metal-Organic Frameworks as Advanced Anode Materials in K- and Na-Ion Batteries.

ACS Appl Mater Interfaces 2021 Oct 22;13(39):46902-46908. Epub 2021 Sep 22.

College of Chemistry, Northeast Normal University, Changchun, Jilin 130024, P. R. China.

A new metal-organic framework {(MeNH)[Co(μ-O)(btb)(py)(HO)]·(DMF)(HO)} () was solvothermal synthesized (Hbtb = 1,3,5-tri(4-carboxylphenyl)benzene, py = pyridine, DMF = N,N-dimethylformamide). shows a (3,6)-connected 3D network with a point symbol of (4·6)(4·6·8) based on the [Co(μ-O)] clusters. The obtained has stable, accessible, dense active sites and can be applied in the potassium- and sodium-ion batteries. Through mixing with single-walled carbon nanotubes, the prepared composite anode material -0.9 achieved a high reversible capability, delivering 416 mAh/g in the potassium-ion batteries and 379 mAh/g in the sodium-ion batteries at 0.05 A/g. The outstanding properties of -0.9 in the batteries demonstrated that this MOFs-based carbon composite is a highly desirable electrode material candidate for high-performance potassium- and sodium-ion batteries.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.1c15356DOI Listing
October 2021

Digoxin exerts anticancer activity on human nonsmall cell lung cancer cells by blocking PI3K/Akt pathway.

Biosci Rep 2021 Oct;41(10)

Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmaceutical Sciences, Tianjin Medical University, Tianjin 300070, China.

Lung cancer remains the leading cause of cancer mortality because of its metastatic potential and high malignancy. The discovery of new applications for old drugs is a shortcut for cancer therapy. We recently investigated the antitumor effect of digoxin, a well-established drug for treating heart failure, against nonsmall cell lung cancer A549 and H1299 cells. Digoxin inhibited the proliferation and colony-forming ability of the two cell lines and arrested the cell cycle at the G0/G1 phase in A549 cells and the G2/M phase in H1299 cells. Mitochondria-mediated apoptosis was induced in A549 cells but not in H1299 cells after treatment with digoxin. Moreover, digoxin inhibited the migration, invasion, adhesion and epithelial-mesenchymal transition of A549 and H1299 cells. Autophagy was induced in both cell lines after treatment with digoxin, with an increase in autophagosome foci. In addition, digoxin inhibited the phosphorylation of Akt, mTOR and p70S6K, signaling molecules of the PI3K/Akt pathway that are known to be involved in tumor cell survival, proliferation, metastasis and autophagy. Our findings suggest that digoxin has the potential to be used for therapy for human nonsmall cell lung cancer, but further evidence is required.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1042/BSR20211056DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8495431PMC
October 2021

Experimental Research on Distribution Characteristics of NO Conversion Efficiency of a Diesel Engine SCR Catalyst.

ACS Omega 2021 Sep 2;6(36):23083-23089. Epub 2021 Sep 2.

Vehicle & Transportation Engineering Institute, Henan University of Science and Technology, Luoyang 471003, China.

An experimental investigation of the distribution characteristics of NO conversion efficiency inside a selective catalytic reduction system is presented in this paper. Wash-coated and extruded vanadium-based selective catalytic reduction catalysts were investigated under an ANR (ammonia nitrogen ratio) of 1.0 and various exhaust gas temperature and flow rate conditions. A moveable sampling probe was located at various measuring points on the exit cross section of the selective catalytic reduction catalyst shell, and the final distribution maps of NO conversion efficiency were obtained by the interpolation of measured data. The experimental result showed that the distribution of NO conversion efficiency of the wash-coated selective catalytic reduction catalyst at the exit cross section was extremely nonuniform and the efficiency of the extruded SCR catalyst was uniform with a higher distribution at the center. A comparison experiment with 180° rotation of the selective catalytic reduction catalysts was implemented, and the efficiency distribution map of the wash-coated selective catalytic reduction catalyst also rotated 180° but that of the extruded selective catalytic reduction catalyst remained unchanged. The uniformity coefficient of the extruded selective catalytic reduction catalyst was higher than that of the wash-coated selective catalytic reduction catalyst used in this study.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsomega.1c02396DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8444220PMC
September 2021

Langerhans cell histiocytosis causing cerebrovascular stenosis leading to stroke in an adult female.

Neurol Sci 2021 Nov 20;42(11):4849-4851. Epub 2021 Sep 20.

Department of Neurology, Neurology Intensive Care Unit, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu, China.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10072-021-05614-3DOI Listing
November 2021

Preparation of Targeted Mitochondrion Nanoscale-Release Peptides and Their Efficiency on Eukaryotic Cells.

J Biomed Nanotechnol 2021 Aug;17(8):1679-1689

Xi'an Medical University, Xi'an 710021, Shaanxi, PR China.

We established a self-decomposable SiO₂ encapsulated mitochondrial targeting short peptide SS31 drug loading system (SiO₂@SS31) to determine its nano-sustained release characteristics in eukaryotic cells. We explored the protection of SiO₂@SS31 on the 661W cells after oxidative injury by H₂O₂. After the drug loading, we detected the morphology of SiO₂@SS31 by transmission electron microscopy (TEM). Moreover, high-pressure liquid chromatography (HPLC) was used to determine the drug capacity and encapsulation efficiency of the nanoparticles. Then, the release curve in vitro was drawn. The 661W cells were cultured in vitro to allow the detection of cytotoxicity by the MTT assay. The SS31loaded nanoscale microspheres labeled with fluorescein isothiocyanate (SiO₂@FITC-SS31) were prepared, and their sustained release effect was detected with intracellular endocytosis, using confocal microscopy and flow cytometry. Within 15 days, the [email protected] nanoparticles were completely decomposed and simultaneously released the SS31 peptide in deionized water and normal saline. Nonetheless, the process was faster in simulated body fluid and serum. The MTT assay suggested that SiO₂@SS31 has sustained protection compared with SS31 in the 661W cells at 48 h. Flow cytometry proved SiO₂@FITC-SS31 could maintain a high level and last longer after 24 h. The SS31 peptide, which has excellent medical application prospects, can be slowly and continuously released from self-decomposable SiO₂ and targeted to concentrate on mitochondria.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1166/jbn.2021.3141DOI Listing
August 2021

Identification of ARHGEF38, NETO2, GOLM1, and SAPCD2 Associated With Prostate Cancer Progression by Bioinformatic Analysis and Experimental Validation.

Front Cell Dev Biol 2021 1;9:718638. Epub 2021 Sep 1.

Department of Urology, Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.

Prostate cancer (PCa) represents one of the most prevalent types of cancers and is a large health burden for men. The pathogenic mechanisms of PCa still need further investigation. The aim of this study was to construct an effective signature to predict the prognosis of PCa patients and identify the biofunctions of signature-related genes. First, we screened differentially expressed genes (DEGs) between PCa and normal control tissues in The Cancer Genome Atlas (TCGA) and GSE46602 datasets, and we performed weighted gene co-expression network analysis (WGCNA) to determine gene modules correlated with tumors. In total, 124 differentially co-expressed genes were retained. Additionally, five genes (ARHGEF38, NETO2, PRSS21, GOLM1, and SAPCD2) were identified to develop the prognostic signature based on TCGA dataset. The five-gene risk score was verified as an independent prognostic indicator through multivariate Cox regression analyses. The expression of the five genes involved in the signature was detected in the Gene Expression Omnibus (GEO), Gene Expression Profiling Interactive Analysis (GEPIA), and Oncomine databases. In addition, we utilized DiseaseMeth 2.0 and MEXPRESS for further analysis and found that abnormal methylation patterns may be a potential mechanism for these five DEGs in PCa. Finally, we observed that these genes, except PRSS21, were highly expressed in tumor samples and PCa cells. Functional experiments revealed that silencing ARHGEF38, NETO2, GOLM1, and SAPCD2 suppressed the proliferation, migration, and invasiveness of PCa cells. In summary, this prognostic signature had significant clinical significance for treatment planning and prognostic evaluation of patients with PCa. Thus, ARHGEF38, NETO2, GOLM1, and SAPCD2 may serve as oncogenes in PCa.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fcell.2021.718638DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8440839PMC
September 2021

TaWRKY13-A Serves as a Mediator of Jasmonic Acid-Related Leaf Senescence by Modulating Jasmonic Acid Biosynthesis.

Front Plant Sci 2021 1;12:717233. Epub 2021 Sep 1.

Ministry of Education Key Laboratory of Molecular and Cell Biology, Hebei Innovation Center for Cell Signaling, College of Life Sciences, Hebei Normal University, Shijiazhuang, China.

Leaf senescence is crucial for crop yield and quality. Transcriptional regulation is a key step for integrating various senescence-related signals into the nucleus. However, few regulators of senescence implicating transcriptional events have been functionally characterized in wheat. Based on our RNA-seq data, we identified a WRKY transcription factor, TaWRKY13-A, that predominately expresses at senescent stages. By using the virus-induced gene silencing (VIGS) method, we manifested impaired transcription of leading to a delayed leaf senescence phenotype in wheat. Moreover, the overexpression () of accelerated the onset of leaf senescence under both natural growth condition and darkness in and . Furthermore, by physiological and molecular investigations, we verified that TaWRKY13-A participates in the regulation of leaf senescence jasmonic acid (JA) pathway. The expression of JA biosynthetic genes, including , was altered in -overexpressing . We also demonstrated that TaWRKY13-A can interact with the promoter of and by using the electrophoretic mobility shift assay (EMSA) and luciferase reporter system. Consistently, we detected a higher JA level in -overexpressing lines than that in Col-0. Moreover, our data suggested that TaWRKY13-A is partially functional conserved with AtWRKY53 in age-dependent leaf senescence. Collectively, this study manifests TaWRKY13-A as a positive regulator of JA-related leaf senescence, which could be a new clue for molecular breeding in wheat.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fpls.2021.717233DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8442999PMC
September 2021

Untargeted metabolomics identifies succinate as a biomarker and therapeutic target in aortic aneurysm and dissection.

Eur Heart J 2021 Sep 17. Epub 2021 Sep 17.

The Institute of Cardiovascular Sciences and Institute of Systems Biomedicine, School of Basic Medical Sciences, Key Laboratory of Molecular Cardiovascular Sciences of Ministry of Education, Health Sciences Center, Peking University, Xueyuan Road NO.38, Haidian District, Beijing 100871, China.

Aims: Aortic aneurysm and dissection (AAD) are high-risk cardiovascular diseases with no effective cure. Macrophages play an important role in the development of AAD. As succinate triggers inflammatory changes in macrophages, we investigated the significance of succinate in the pathogenesis of AAD and its clinical relevance.

Methods And Results: We used untargeted metabolomics and mass spectrometry to determine plasma succinate concentrations in 40 and 1665 individuals of the discovery and validation cohorts, respectively. Three different murine AAD models were used to determine the role of succinate in AAD development. We further examined the role of oxoglutarate dehydrogenase (OGDH) and its transcription factor cyclic adenosine monophosphate-responsive element-binding protein 1 (CREB) in the context of macrophage-mediated inflammation and established p38αMKOApoe-/- mice. Succinate was the most upregulated metabolite in the discovery cohort; this was confirmed in the validation cohort. Plasma succinate concentrations were higher in patients with AAD compared with those in healthy controls, patients with acute myocardial infarction (AMI), and patients with pulmonary embolism (PE). Moreover, succinate administration aggravated angiotensin II-induced AAD and vascular inflammation in mice. In contrast, knockdown of OGDH reduced the expression of inflammatory factors in macrophages. The conditional deletion of p38α decreased CREB phosphorylation, OGDH expression, and succinate concentrations. Conditional deletion of p38α in macrophages reduced angiotensin II-induced AAD.

Conclusion: Plasma succinate concentrations allow to distinguish patients with AAD from both healthy controls and patients with AMI or PE. Succinate concentrations are regulated by the p38α-CREB-OGDH axis in macrophages.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1093/eurheartj/ehab605DOI Listing
September 2021

Quantitative lung perfusion blood volume (PBV) using dual energy CT (DECT)-based effective atomic number (Z ) imaging.

Med Phys 2021 Sep 14. Epub 2021 Sep 14.

Department of Medical Physics, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, 53705, USA.

Background: Iodine material images (aka iodine basis images) generated from dual energy CT (DECT) have been used to assess potential perfusion defects in the pulmonary parenchyma. However, iodine material images do not provide the needed absolute quantification of the pulmonary blood pool, as materials with effective atomic numbers (Z ) different from those of basis materials may also contribute to iodine material images, thus confounding the quantification of perfusion defects.

Purpose: The purposes of this work were to (i) demonstrate the limitations of iodine material images in pulmonary perfusion defect quantification and (ii) develop and validate a new quantitative biomarker using effective atomic numbers derived from DECT images.

Methods: The quantitative relationship between the perfusion blood volume (PBV) in pulmonary parenchyma and the effective atomic number (Z ) spatial distribution was studied to show that the desired quantitative PBV maps are determined by the spatial maps of Z as PBV (x) = a Z (x) + b, where a, b, and β are three constants. Namely, quantitative PBV is determined by Z images instead of the iodine basis images. Perfusion maps were generated for four human subjects to demonstrate the differences between conventional iodine material image-based PBV (PBV ) derived from two-material decompositions and the proposed PBV method.

Results: Among patients with pulmonary emboli, the proposed PBV maps clearly show the perfusion defects while the PBV maps do not. Additionally, when there are no perfusion defects present in the derived PBV maps, no pulmonary emboli were diagnosed by an experienced thoracic radiologist.

Conclusion: Effective atomic number based quantitative PBV maps provide the needed sensitive and specific biomarker to quantify pulmonary perfusion defects. This article is protected by copyright. All rights reserved.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/mp.15227DOI Listing
September 2021
-->