Publications by authors named "Ke Chen"

1,251 Publications

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Physiological effects induced by aluminium and fluoride stress in tall fescue (Festuca arundinacea Schreb).

Ecotoxicol Environ Saf 2022 Jan 11;231:113192. Epub 2022 Jan 11.

College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu Province 225009, PR China. Electronic address:

Aluminium (Al) and fluoride (F) are phytotoxic elements that can inhibit plant growth and development. Al and F can react with each other to form complexes in the soil which will induce alteration of toxicity of single element. However, the mechanisms of plant response to aluminium fluoride induced toxicity are not very clear. In the present study, tall fescue (Festuca arundinacea Schreb) cultivar 'Houndog 5' was treated by 0, 0.4, 4, 20 mg·L Al(SO) and 0, 0.5, 5 mg·L NaF, respectively. After 25 days of treatment, leaf samples were collected for physiological evaluation. The results showed that several forms of Al-OH and Al-F complexes such as Al(OH), AlOH, Al(OH), Al(OH), Al(OH), Al(OH), AlF, AlF, AlF and AlF were formed in Al and F combined solution. The nutrient uptake including Al, P and K were improved by Al and F. Under Al stress, the MDA (malondialdehyde) content and EL (electrolyte leakage) dramatically increased after high concentration of F treatment, while relative low concentration of F induced decrease of MDA content and EL. On the contrary, chlorophyll content decreased significantly after high concentration of F treatment. The photosynthesis efficiency parameters, including φ (Fv/Fm), δ and PI, decreased remarkably after high concentration of Al and F treatment. However, L-band incresed after high concentration of Al and F treatment. The results of correlation analysis showed that MDA content and EL negatively correlated with other indexes, and Al-F complex significantly correlated with MDA, Pro and EL but negatively correlated with Chl and φ. These results suggested that low concentration of F could alleviate the damage induced by Al stress in tall fescue, but high concentration of Al and F combined solution had negative effects on the growth and development of tall fescue.
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http://dx.doi.org/10.1016/j.ecoenv.2022.113192DOI Listing
January 2022

Observation of second sound in graphite over 200 K.

Nat Commun 2022 Jan 12;13(1):285. Epub 2022 Jan 12.

Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.

Second sound refers to the phenomenon of heat propagation as temperature waves in the phonon hydrodynamic transport regime. We directly observe second sound in graphite at temperatures of over 200 K using a sub-picosecond transient grating technique. The experimentally determined dispersion relation of the thermal-wave velocity increases with decreasing grating period, consistent with first-principles-based solution of the Peierls-Boltzmann transport equation. Through simulation, we reveal this increase as a result of thermal zero sound-the thermal waves due to ballistic phonons. Our experimental findings are well explained with the interplay among three groups of phonons: ballistic, diffusive, and hydrodynamic phonons. Our ab initio calculations further predict a large isotope effect on the properties of thermal waves and the existence of second sound at room temperature in isotopically pure graphite.
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http://dx.doi.org/10.1038/s41467-021-27907-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8755757PMC
January 2022

Preparation and antibacterial properties of an [email protected]/GelMA composite hydrogel.

Biomed Mater 2022 Jan 11. Epub 2022 Jan 11.

Chongqing University of Science and Technology, 20# east road, college town, Chongqing, 401331, CHINA.

Pure gelatin hydrogels lack antibacterial function and have poor mechanical properties, which restrict their application in wound dressings. In this study, nanosized silver bromide-doped mesoporous silica ([email protected]) microspheres with hollow structures were prepared by a modified Stober method. The novel microspheres can not only release silver ions to treat bacteria but also release drugs to treat skin wound. Furthermore, [email protected] microspheres were modified with propyl methacrylate, incorporated into methacrylated gelatin (GelMA), and crosslinked by UV light to prepare [email protected]/GelMA dressings consisting of composite hydrogels. The results showed that the [email protected] microspheres could enhance the mechanical properties of the hydrogels. With the increase in the [email protected] concentration from 0.5 to 1 mg/mL, the dressings demonstrated effective antimicrobial activity against both Staphylococcus aureus and Escherichia coli. Furthermore, full-thickness skin wounds in vivo wound healing studies with Sprague-Dawley rats were evaluated. When treated with [email protected]/GelMA containing 1 mg/mL [email protected], only 15% of the wound area left on day 10. Histology results also showed the epidermal and dermal layers were better organized. These results suggest that [email protected]/GelMA-based dressing materials could be promising candidates for wound dressings.
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http://dx.doi.org/10.1088/1748-605X/ac49f7DOI Listing
January 2022

Effects of lower body positive pressure treadmill on functional improvement in knee osteoarthritis: A randomized clinical trial study.

World J Clin Cases 2021 Dec;9(34):10604-10615

Department of General Medicine, The Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou 510700, Guangdong Province, China.

Background: Knee joint pain and stiffness are the two main symptoms of knee osteoarthritis (OA) and thus restrict a patient's activities, such as walking and walking up and downstairs. The lower body positive pressure (LBPP) treadmill as one of the emerging body weight support system devices brings new hope for exercise-related rehabilitation for knee OA patients.

Aim: To investigate the biomechanical effects and the subjective clinical assessment of LBPP treadmill walking exercise when compared with conventional therapy in mild to moderate knee OA patients.

Methods: Eighteen patients with mild-to-moderate knee OA were recruited in this randomized controlled trial (RCT) study. The eligible knee OA patients were randomly assigned to two groups: LBPP and control groups. The patients in the LBPP group performed an LBPP walking training program for 30 min/session per day, 6 d per week for 2 wk whereas the patients in the control group performed walking on the ground for the same amount. All patients underwent clinical assessments and three-dimensional gait analysis at pre- and 2-wk post-treatment.

Results: The Western Ontario and McMaster Universities Arthritis Index and visual analog scale scores in both the LBPP group and control group were found to decrease significantly at the post-treatment point than the pre-treatment point (LBPP: 70.25 ± 13.93 40.50 ± 11.86; 3.88 ± 0.99 1.63 ± 0.52; control: 69.20 ± 8.88 48.10 ± 8.67; 3.80 ± 0.79 2.60 ± 0.70, < 0.001). Moreover, compared with the control group, the LBPP group showed more improvements in walking speed ( 0.007), stride length ( = 0.037), and knee range of motion ( = 0.048) during walking, which represented more improvement in walking ability.

Conclusion: The results of our RCT study showed that the LBPP group has a greater effect on improving gait parameters than the conventional group, although there was no significant advantage in clinical assessment. This finding indicates that LBPP treadmill walking training might be an effective approach for alleviating pain symptoms and improving lower extremity locomotion in mild to moderate knee OA patients.
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http://dx.doi.org/10.12998/wjcc.v9.i34.10604DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8686144PMC
December 2021

Self-Organized Fractal Structures on Plasma-Exposed Silver Surface.

Front Chem 2021 24;9:816811. Epub 2021 Dec 24.

The Jiangsu Key Laboratory of Power Transmission and Distribution Equipment Technology, Hohai University, Changzhou, China.

Planar fractal microstructure is observed on the silver film treated by positive corona discharge for the first time. Due to the abundant positive ions driven by the electrical field of positive polarity, surface modification is mainly induced by the plasma oxidation effect, resulting in a large scale of dendritic pattern with self-similarity and hierarchy. In contrast, negative ions dominate the plasma-film interaction under negative corona discharge condition, leading to a different surface morphology without fractal characteristics. A growth model based on the modified diffusion-limited aggregation (DLA) theory is proposed to describe the formation of the dendritic fractal structure, whilst the physics behind is attributed to the electric field directed diffusion of the positive ions around the surface roughness. Numerical simulation verifies the high density of the hot spot in the dendritic pattern, which may enable potential applications in fractal photonic metamaterials.
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http://dx.doi.org/10.3389/fchem.2021.816811DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8738162PMC
December 2021

Effect of passage number of genetically modified TGF-β3 expressing primary chondrocytes on the chondrogenesis of ATDC5 cells in a 3D coculture system.

Biomed Mater 2022 Jan 6. Epub 2022 Jan 6.

Department of Joint Surgery, the First Affiliated Hospital of Guangzhou Medical University, No151, Yanjiang road, Guangzhou, Guangdong, 510120, CHINA.

Due to the lack of blood vessels, nerves and lymphatics, articular cartilage is difficult to repair once damaged. Tissue engineering is considered to be a potential strategy for cartilage regeneration. Successful tissue engineering strategies depend on the effective combination of biomaterials, seed cells and biological factors. In our previous study, a genetically modified coculture system with chondrocytes and ATDC5 cells in an alginate hydrogel has exhibited a superior ability to enhance chondrogenesis. In this study, we further evaluated the influence of chondrocytes at various passages on chondrogenesis in the coculture system. The results demonstrated that transfection efficiency was hardly influenced by the passage of chondrocytes. The coculture system with passage 5 (P5) chondrocytes had a better effect on chondrogenesis of ATDC 5 cells, while chondrocytes in this coculture system presented higher levels of dedifferentiation than other groups with P1 or P3 chondrocytes. Therefore, P5 chondrocytes were shown to be more suitable for the coculture system, as they accumulated in sufficient cell numbers with more passages and had a higher level of dedifferentiation, which was prone to form a favorable niche for chondrogenesis of ATDC5 cells. This study may provide fresh insights for future cartilage tissue engineering strategies with a combination of a coculture system and advanced biomaterials.
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http://dx.doi.org/10.1088/1748-605X/ac489eDOI Listing
January 2022

High-Sensitivity Silicon Cantilever-Enhanced Photoacoustic Spectroscopy Analyzer with Low Gas Consumption.

Anal Chem 2022 Jan 6;94(2):1151-1157. Epub 2022 Jan 6.

School of Optoelectronic Engineering and Instrumentation Science, Dalian University of Technology, Dalian, Liaoning 116024, China.

A silicon cantilever-enhanced photoacoustic spectroscopy (PAS)-based trace gas analyzer with low gas consumption is presented. A silicon cantilever-based fiber-optic Fabry-Perot (F-P) interferometric acoustic sensor with a compact structure and high sensitivity is designed for photoacoustic signal detection. The non-resonant photoacoustic cell (PAC) is a cylindrical copper tube with a volume of 0.56 mL. A near-infrared laser with a center wavelength of 1532.83 nm amplified using an erbium-doped fiber application amplifier is used as the excitation light. The wavelength modulation spectroscopy (WMS) technique is employed in the present work for second-harmonic photoacoustic signal detection. The experimental results show that the minimum detection limit of CH is 199.8 parts per trillion (ppt) with an average time of 60 s. The normalized noise equivalent absorption coefficient is calculated as 1.72 × 10 cm W/Hz. Furthermore, the proposed silicon cantilever-enhanced non-resonant PAS-based gas analyzer can not only analyze the gas concentration in a closed small-capacity PAC with low gas consumption but also detect target gas leakage in real time at a long distance.
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http://dx.doi.org/10.1021/acs.analchem.1c04309DOI Listing
January 2022

Alzheimer disease effects of different stages on intestinal flora: A protocol for systematic review and meta-analysis.

Medicine (Baltimore) 2021 Dec;100(52):e28462

College of Traditional Chinese Medicine, Shandong University of Traditional Chinese Medicine, Jinan, Shandong Province, China.

Background: Alzheimer disease (AD) is a common degenerative disease of the central nervous system that can be divided into 3 stages, according to the degree of cognitive impairment. The clinical manifestations are cognitive dysfunction and memory loss, impacting the daily activities of the affected individuals. In recent years, studies have demonstrated a relationship between intestinal flora and AD. However, no meta-analysis has documented the correlation between AD and intestinal flora, to the best of our knowledge. Herein, we sought to assess the correlation between different stages of AD and intestinal flora. A systematic and comprehensive understanding of this relationship is of great significance for developing prevention and treatment strategies against AD.

Methods: A comprehensive search of the medical literature in Chinese and English language was performed in databases, such as PubMed, EBSCO, CNKI, web of science, WanFang, Cochrane Library, and CBM databases. Pre-defined search strategies were used to retrieve clinical studies of Alzheimer disease and gut microbiota. The included studies were independently analyzed by the 2 researchers who extracted the data. The quality of the data was evaluated according to the "Cochrane system evaluator manual." Finally, Endnote and RevMan software were used for systematic regression and meta-analysis of evidence.

Results: We documented the intestinal flora changes in the 3 stages of Alzheimer disease, according to currently available clinical evidence, and revealed the correlation between the abundance and diversity of flora and treatment efficacy. These findings are essential for developing new strategies for the prevention and treatment of Alzheimer disease.

Inplasy Registration Number: INPLASY2021100093.

Ethics And Dissemination: Since all data utilized in this systematic review and meta-analysis are published, ethical approval was not needed.
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http://dx.doi.org/10.1097/MD.0000000000028462DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8718226PMC
December 2021

Nanocone-Shaped Carbon Nanotubes Field-Emitter Array Fabricated by Laser Ablation.

Nanomaterials (Basel) 2021 Nov 29;11(12). Epub 2021 Nov 29.

CAS Key Laboratory of Nanophotonic Materials and Devices, CAS Key Laboratory of Standardization and Measurement for Nanotechnology, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing 100190, China.

The nanocone-shaped carbon nanotubes field-emitter array (NCNA) is a near-ideal field-emitter array that combines the advantages of geometry and material. In contrast to previous methods of field-emitter array, laser ablation is a low-cost and clean method that does not require any photolithography or wet chemistry. However, nanocone shapes are hard to achieve through laser ablation due to the micrometer-scale focusing spot. Here, we develop an ultraviolet (UV) laser beam patterning technique that is capable of reliably realizing NCNA with a cone-tip radius of ≈300 nm, utilizing optimized beam focusing and unique carbon nanotube-light interaction properties. The patterned array provided smaller turn-on fields (reduced from 2.6 to 1.6 V/μm) in emitters and supported a higher (increased from 10 to 140 mA/cm) and more stable emission than their unpatterned counterparts. The present technique may be widely applied in the fabrication of high-performance CNTs field-emitter arrays.
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http://dx.doi.org/10.3390/nano11123244DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8707308PMC
November 2021

The Regulatory Effects and the Signaling Pathways of Natural Bioactive Compounds on Ferroptosis.

Foods 2021 Dec 1;10(12). Epub 2021 Dec 1.

Centre for Advanced Medical Research and Training (CAMRET), Department of Medical Biochemistry, Usmanu Danfodiyo University Sokoto, Sokoto 840232, Nigeria.

Natural bioactive compounds abundantly presented in foods and medicinal plants have recently received a remarkable attention because of their various biological activities and minimal toxicity. In recent years, many natural compounds appear to offer significant effects in the regulation of ferroptosis. Ferroptosis is the forefront of international scientific research which has been exponential growth since the term was coined. This type of regulated cell death is driven by iron-dependent phospholipid peroxidation. Recent studies have shown that numerous organ injuries and pathophysiological processes of many diseases are driven by ferroptosis, such as cancer, arteriosclerosis, neurodegenerative disease, diabetes, ischemia-reperfusion injury and acute renal failure. It is reported that the initiation and inhibition of ferroptosis plays a pivotal role in lipid peroxidation, organ damage, neurodegeneration and cancer growth and progression. Recently, many natural phytochemicals extracted from edible plants have been demonstrated to be novel ferroptosis regulators and have the potential to treat ferroptosis-related diseases. This review provides an updated overview on the role of natural bioactive compounds and the potential signaling pathways in the regulation of ferroptosis.
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http://dx.doi.org/10.3390/foods10122952DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8700948PMC
December 2021

Comparison of laparoscopic proximal gastrectomy with double-tract reconstruction and laparoscopic total gastrectomy for proximal gastric cancer with stage cT1-2.

Medicine (Baltimore) 2021 Dec;100(51):e28115

Department of General and Minimally Invasive Surgery, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, China.

Abstract: This study aimed to evaluate the feasibility and nutritional benefits of laparoscopic proximal gastrectomy (LPG) with double-tract reconstruction (DTR) in comparison with laparoscopic total gastrectomy (LTG).The demographic, clinical, and pathological data and postoperative nutritional status of patients undergoing LPG with DTR (n = 21) or LTG (n = 26) at Sir Run Run Shaw Hospital between January 2016 and January 2019 were retrospectively reviewed and compared.The operative time in the LPG group was slightly longer than that in the LTG group; however, the difference was not statistically significant. Blood loss was not significantly different between groups. The mean number of retrieved lymph nodes was higher in the LTG group than in the LPG group (P = .02). The time to first flatus, postoperative hospital stay, and postoperative complications were comparable between the groups. During the 3-year postoperative follow-up, a statistically significant decrease in hemoglobin level was observed in the LTG group. There were no differences between the two groups of patients before and after the operation regarding albumin levels. The mean vitamin B12 level was higher in the LPG group than in the LTG group from 12 to 18 months postoperatively.LPG with DTR is an acceptable procedure for patients with upper gastric cancer. LPG with DTR has numerous potential advantages in preserving the physiological and nutritional functions of the remnant stomach and the conservation of the gastric reservoir.
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http://dx.doi.org/10.1097/MD.0000000000028115DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8702284PMC
December 2021

Retrospective Analysis of the Clinical Efficacy of Early Goal-Directed Therapy Combined with Meticulous Nursing Intervention in Patients with Posttraumatic Sepsis.

J Healthc Eng 2021 13;2021:6706464. Epub 2021 Dec 13.

Department of Cardiology, The Third Hospital of Hebei Medical University, Shijiazhuang 050051, Hebei, China.

Objective: To explore the intervention effect of early goal-directed therapy (EGDT) combined with meticulous nursing on patients with posttraumatic sepsis.

Methods: The data of 50 patients with posttraumatic sepsis undergoing EGDT in the emergency department of our hospital from January 2020 to December 2020 were retrospectively analyzed. According to different nursing methods, they were divided into control group ( = 25) with routine nursing measures and observation group ( = 25) with meticulous nursing measures. The application effect of the two nursing modes was scientifically evaluated.

Results: No statistical differences in general data were found between the two groups ( > 0.05). After 6 h of intervention, the circulatory function, oxygenation function, and renal function of both groups were better than those before intervention, and central venous pressure (CVP), mean arterial pressure (MAP), blood oxygen (PaO), oxygenation index (PaO/FiO), central venous oxygen saturation (ScvO), and urine volume in the observation group were notably higher than those in the control group ( < 0.05). The heart rate (HR), serum creatinine (SCr), and blood lactic acid in the observation group were notably lower than those in the control group ( < 0.05). The 28-day survival rate and quality of life after intervention in the observation group were notably higher than those in the control group, with obvious differences between the two groups ( < 0.05).

Conclusion: Meticulous nursing intervention for patients with posttraumatic sepsis undergoing EGDT can effectively improve the body's functional indexes, which is superior to the routine nursing in controlling the patients' condition, improving the survival rate and quality of life after intervention, and ensuring the clinical treatment effect. Therefore, it is worthy of promotion.
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http://dx.doi.org/10.1155/2021/6706464DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8687773PMC
December 2021

RORα and REV-ERBα are Associated With Clinicopathological Parameters and are Independent Biomarkers of Prognosis in Gastric Cancer.

Technol Cancer Res Treat 2021 Jan-Dec;20:15330338211039670

36639The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, People's Republic of China.

Retinoid-related orphan receptor alpha (RORα) and nuclear receptor subfamily 1 group D member 1 (REV-ERBα) play critical roles in many human cancers. Whether RORα and REV-ERBα expression levels are associated with clinical characteristics are poorly understood, and they may be independent predictors of overall survival (OS) and progression-free survival (PFS) in gastric cancer (GC). This study aimed to investigate the correlation of RORα and REV-ERBα expression levels with clinicopathological parameters, OS, and PFS in GC. Immunohistochemistry and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) were employed to assess the expression levels of RORα and REV-ERBα, which were downregulated in GC tissues compared with normal gastric tissues ( < .001;  < .001) and were associated with several clinicopathological parameters, including histological grade ( = .032;  < .001), preoperative carcinoembryonic antigen (CEA) levels ( = .004;  < .001), and tumor-node-metastasis (TNM) stage ( = .015;  < .001). Additionally, low RORα and REV-ERBα expression levels were associated with poor OS and PFS in GC patients, respectively ( < .001;  = .001). Furthermore, univariate Cox regression model analysis showed that histological grade ( < .001;  < .001), preoperative CEA levels ( < .001;  = .001), TNM stage ( < .001;  < .001), lymph node metastasis ( = .002;  = .002), RORα expression levels ( = .001;  < .001), and REV-ERBα expression levels ( < .001;  = .001) were associated with OS and PFS in GC. Multivariate Cox regression model analysis indicated that RORα expression levels and REV-ERBα expression levels are independent factors of OS and PFS in GC. Besides, RORα and REV-ERBα expression may be positively correlated (χ = 6.835;  = .009), and GC patients with both high RORα and REV-ERBα expression levels had the best prognosis. In conclusion, RORα and REV-ERBα may coparticipate in tumor activities and show potential to estimate the prognosis of GC.
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http://dx.doi.org/10.1177/15330338211039670DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8721360PMC
December 2021

A Low-Temperature Carbon Encapsulation Strategy for Stable and Poisoning-Tolerant Electrocatalysts.

Small Methods 2021 Nov 14;5(11):e2100937. Epub 2021 Sep 14.

Key Laboratory of Material Chemistry for Energy Conversion and Storage (Huazhong University of Science and Technology), Ministry of Education, Hubei Key Laboratory of Material Chemistry and Service Failure, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan, 430074, P. R. China.

Carbon encapsulation is an effective strategy for enhancing the durability of Pt-based electrocatalysts for the oxygen reduction reaction (ORR). However, high-temperature treatment is not only energy-intensive but also unavoidably leads to possible aggregation. Herein, a low-temperature polymeric carbon encapsulation strategy (≈150 °C) is reported to encase Pt nanoparticles in thin and amorphous carbonaceous layers. Benefiting from the physical confinement effect and enhanced antioxidant property induced by the surface carbon species, significantly improved stabilities can be achieved for polymeric carbon species encapsulated Pt nanoparticles ([email protected]/C). Particularly, a better antipoisoning capability toward CO, SO , and PO is observed in the case of [email protected]/C. To minimize the thickness of the catalyst layer and reduce the mass transfer resistance, the high mass loading [email protected]/C (40 wt%) is prepared and applied to high-temperature polymer electrolyte membrane fuel cells (HT-PEMFCs). At 160 °C, a peak power density of 662 mW cm is achieved with 40% [email protected]/C cathode in H -O HT-PEMFCs, which is superior to that with 40% Pt/C cathode. The facile strategy provides guidance for the synthesis of highly durable carbon encapsulated noble metal electrocatalysts toward ORR.
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http://dx.doi.org/10.1002/smtd.202100937DOI Listing
November 2021

Period circadian clock 3 inhibits palmitic acid-induced oxidative stress and inflammatory factor secretion in podocytes.

Zhong Nan Da Xue Xue Bao Yi Xue Ban 2021 Nov;46(11):1177-1186

Department of Endorcrinology, Third Xiangya Hospital, Central South University, Changsha 410013, China.

Objectives: High fat-induced podocyte injury is one of the important factors leading to obesity related nephropathy (ORG), but the mechanism is not clear. This study aims to explore the mechanism of period circadian clock 3 (PER3) in the oxidative stress and inflammation induced by palmitic acid (PA) in podocytes.

Methods: The C57BL/6J mice were fed with chow and high-fat diet for 16 weeks. The PER3 expression in kidney tissues were detected in the normal body weight group and the obesity group. The PER3 mRNA and protein expression were detected after the podocytes were induced with different concentrations (0, 50, 150 and 300 μmol/L) of PA for 48 h. The PER3 mRNA and protein expression were detected after the podocytes were induced with 150 μmol/L PA for 0, 24, 36, and 48 h. Triglyceride (TG) levels were examined in the PA group, the adenovirus (ad)-PER3+PA group, and the siRNA-PER+PA group after the podocytes were transfected by Ad-PER3 or small interfering RNA (siRNA)-PER3 for 48 h and subsequently were induced with 150 μmol/L PA for 48 h. The differential gene expression was detected using RNA sequencing (RNA-seq) after podocytes were transfected by siRNA-PER3 (siRNA-PER3 group) and siRNA-control (siRNA-control group), respectively. The mRNA levels of nephrin, podocin, podocalyxin, podoplanin, superoxide dismutase 1 (SOD1), glutathione peroxidase 1 (GPX1), catalase (CAT), and the levels of malondialdehyde (MDA), glutathione (GSH), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1β (IL-1β) and interleukin-2 (IL-2) were detected after podocytes were transfected with Ad-PER3 or Ad-control for 48 h and then they were induced by 150 μmol/L PA for 48 h.

Results: The PER3 was down-regulated in the obesity group compared with the normal body weight group (<0.05), and the PER3 was significantly down-regulated after the podocytes were treated with 150 μmol/L for 48 h compared with 0, 24, and 36 h (all <0.01). The TG contents were significantly decreased in the Ad-PER3+PA group compared with the PA group (<0.05). On the contrary, TG contents were increased in the siRNA-PER3+PA group compared with the PA group (<0.05). The RNA-seq results showed that: compared with the siRNA-control group, the differential genes in the siRNA-PER3 group were enriched in different pathways including oxidative phosphorylation, TNF signaling pathway, extracellular matrix receptor interaction, fatty acid metabolism, and fatty acid degradation (all <0.05). The podocyte marker genes (nephrin, podocin, podocalyxin and podoplanin), oxidative stress (SOD1, GPX1, CAT and GSH), and inflammation factors (TNF-α, IL-6, IL-1β and IL-2) were significantly down-regulated in the Ad-PER3+PA group compared with the PA group (all <0.05).

Conclusions: PER3 can decrease the PA-induced oxidative stress and inflammatory factor secretion via inhibiting the lipogenesis in podocytes.
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http://dx.doi.org/10.11817/j.issn.1672-7347.2021.210019DOI Listing
November 2021

Portable and Intelligent Urine Glucose Analyzer Based on a CdTe [email protected] Aerogel Circular Array Sensor.

ACS Omega 2021 Dec 19;6(48):32655-32662. Epub 2021 Nov 19.

School of Mechanical Engineering, and Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, Southeast University, Nanjing 211189, China.

Diabetes is a public health problem characterized by hyperglycemia, high mortality, and morbidity. A simple, rapid, and sensitive glucose detection method for diabetes screening and health self-management of patients with diabetes is of great significance. Therefore, an attractive urine glucose (UG) analyzer with advantages of fastness, sensitivity, and portability was developed. A cadmium telluride quantum dots (CdTe QDs)@glucose oxidase (GOx) aerogel circular array sensor can emit visible red fluorescence when excited by a 365 nm ultraviolet light source inside the analyzer. When urine samples containing glucose were dropped onto the sensor, glucose was oxidized by GOx to produce hydrogen peroxide (HO), which quenched the red fluorescence of CdTe QDs. The fluorescence images of the sensor were obtained using a CCD camera, and the linear relationship between the glucose concentration and the gray value of the fluorescence image was established. The analyzer shows good sensitivity (LOD, 0.12 mM) with a wide linear range of 0.12-26 mM. Based on the linear relation, the software of the analyzer was written in the C++ language, which can automatically give the gray value of the image and the corresponding glucose concentration. The UG analyzer was used for the detection of a large number clinical samples and compared with a variety of UG test papers, which all showed good detection performance. The novel analyzer we proposed has an important significance in the screening of diabetes and the self-management of diabetic patients.
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http://dx.doi.org/10.1021/acsomega.1c03449DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8655949PMC
December 2021

Increased Circulating Levels of Ectodysplasin A in Newly Diagnosed Type 2 Diabetic Patients.

Front Endocrinol (Lausanne) 2021 9;12:737624. Epub 2021 Nov 9.

Department of Endocrinology, Affiliated Hospital of Jiangsu University, Zhenjiang, China.

Objective: Ectodysplasin A (EDA), a newly discovered hepatokine, has recently been considered to be closely related to glycolipid metabolism disorders, but the pathophysiological effects of EDA are still poorly understood. This study was the first time to determine the level of serum EDA in newly diagnosed type 2 diabetes mellitus (T2DM) patients, and to explore the relationships between serum EDA levels and various metabolic indexes.

Methods: A total of 184 subjects were enrolled in the study, including 92 subjects with newly diagnosed T2DM and 92 subjects with age- and sex-matched normal glucose tolerance (NGT). Serum EDA levels were determined using enzyme-linked immunosorbent assay (ELISA). Oral glucose tolerance test, glycosylated hemoglobin c (HbA1c), and insulin were also measured.

Results: Serum EDA levels were significantly increased in the T2DM group than in the NGT group (359.91 ± 117.99 vs. 265.82 ± 86.51 pg/ml, < 0.001). Serum EDA levels were positively correlated with body mass index (BMI), waist-to-hip ratio (WHR), fasting plasma glucose (FPG), HbA1c, 2-hour postprandial plasma glucose (2hPG), fasting plasma insulin (FIns), fasting C peptide (FCP), triglyceride (TG), HOMA-IR, and negatively correlated with high-density lipoprotein cholesterol (HDL-c) and HOMA-β ( < 0.05). Multiple stepwise regression analysis demonstrated that 2hPG and FIns were independent influencing factors of serum EDA level ( < 0.05). Logistic regression analysis showed that serum EDA level was significantly independently correlated with T2DM ( < 0.05).

Conclusions: Serum EDA levels are significantly higher in T2DM patients, suggesting that EDA may play a role in the occurrence and development of T2DM.
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http://dx.doi.org/10.3389/fendo.2021.737624DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8630655PMC
November 2021

CircSamd4: A novel biomarker for predicting vascular calcification.

J Clin Lab Anal 2022 Jan 29;36(1):e24156. Epub 2021 Nov 29.

Wuxi Clinical College of Anhui Medical University, Wuxi, China.

Background: Vascular calcification (VC) is usually associated with cardiovascular diseases (CVDs), which are one of the main causes of mortality in the world. This study aimed to analyze the expression of circular RNAs (circRNAs) in patients with VC and to evaluate biomarkers for the diagnosis of VC.

Methods: Calcified human aortic endothelial cells (HAECs) and the calcification in mouse aorta were detected by qRT-PCR. Subsequently, this was verified in the plasma of patients with coronary artery calcification (CAC). The plasma of 40 patients in the control group and 31 patients in the calcified group were detected by quantitative reverse transcription polymerase chain reaction (qRT-PCR) to detect the level of circSamd4a in the blood. The diagnostic value was evaluated by logistic regression analysis and the working characteristics of subjects.

Results: In the HAECs, the qRT-PCR showed a significant decrease in the level of circSamd4a expression in the calcification group compared to the control group (p < 0.05). The calcified mouse aorta showed the same trend for circSamd4a expression, wherein the difference was statistically significant (p < 0.05); the expression of circSamd4a was significantly downregulated in the plasma of patients with VC (p < 0.01). The receiver operating characteristic (ROC) curves of circSamd4a in patients with VC and control group showed that the area under the curve (AUC) was 0.81 (95% CI: 0.707-0.913; p < 0.001).

Conclusion: CircSamd4a showed a stable downward trend in different specimens, and had significant advantages as a biomarker for diagnosis of VC.
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http://dx.doi.org/10.1002/jcla.24156DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8761455PMC
January 2022

Stable Field Emission from Vertically Oriented SiC Nanoarrays.

Nanomaterials (Basel) 2021 Nov 11;11(11). Epub 2021 Nov 11.

Henan Institute of Advanced Technology, Zhengzhou University, Zhengzhou 450001, China.

Silicon carbide (SiC) nanostructure is a type of promising field emitter due to high breakdown field strength, high thermal conductivity, low electron affinity, and high electron mobility. However, the fabrication of the SiC nanotips array is difficult due to its chemical inertness. Here we report a simple, industry-familiar reactive ion etching to fabricate well-aligned, vertically orientated SiC nanoarrays on 4H-SiC wafers. The as-synthesized nanoarrays had tapered base angles >60°, and were vertically oriented with a high packing density >10 mm and high-aspect ratios of approximately 35. As a result of its high geometry uniformity-5% length variation and 10% diameter variation, the field emitter array showed typical turn-on fields of 4.3 V μm and a high field-enhancement factor of ~1260. The 8 h current emission stability displayed a mean current fluctuation of 1.9 ± 1%, revealing excellent current emission stability. The as-synthesized emitters demonstrate competitive emission performance that highlights their potential in a variety of vacuum electronics applications. This study provides a new route to realizing scalable field electron emitter production.
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http://dx.doi.org/10.3390/nano11113025DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8622368PMC
November 2021

Effect of Drawing Parameters on the Properties of Polypropylene/Inorganic Particles Composites by Solid-State Die Drawing.

Polymers (Basel) 2021 Nov 12;13(22). Epub 2021 Nov 12.

National Special Superfine Powder Engineering Research Center of China, School of Chemistry and Chemical Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.

Die drawing is an effective method for improving the properties of polymer. In this work, polypropylene (PP)/inorganic particle composites were fabricated by a solid-state die drawing process to investigate the effects of drawing parameters, such as inorganic particles types, drawing temperature, and drawing speed, on the thermal properties, microstructure, and mechanical behavior of the drawn composites. The mechanical properties of the material were significantly improved through this processing method. For the drawn PP/inorganic particle composites with 45 wt% CaCO, when the drawing speed was 2.0 m/min and the drawing temperature was 110 °C, the density of the drawn composites reached the lowest at 1.00 g/cm. At this time, the tensile strength, flexural strength, and impact strength of the drawn composites were 128.32 MPa, 77.12 MPa, and 170.42 KJ/m, respectively. This work provides a new strategy for the preparation of lightweight and high-strength PP-based composites, which have broad application prospects in the field of engineering and structural materials.
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http://dx.doi.org/10.3390/polym13223913DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8624137PMC
November 2021

On a Variational and Convex Model of the Blake-Zisserman Type for Segmentation of Low-Contrast and Piecewise Smooth Images.

J Imaging 2021 Oct 28;7(11). Epub 2021 Oct 28.

Liverpool Centre of Mathematics for Healthcare and Centre for Mathematical Imaging Techniques, Department of Mathematical Sciences, University of Liverpool, Liverpool L69 7ZL, UK.

This paper proposes a new variational model for segmentation of low-contrast and piecewise smooth images. The model is motivated by the two-stage image segmentation work of Cai-Chan-Zeng (2013) for the Mumford-Shah model. To deal with low-contrast images more effectively, especially in treating higher-order discontinuities, we follow the idea of the Blake-Zisserman model instead of the Mumford-Shah. Two practical ideas are introduced here: first, a convex relaxation idea is used to derive an implementable formulation, and second, a game reformulation is proposed to reduce the strong dependence of coupling parameters. The proposed model is then analysed for existence and further solved by an ADMM solver. Numerical experiments can show that the new model outperforms the current state-of-the-art models for some challenging and low-contrast images.
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http://dx.doi.org/10.3390/jimaging7110228DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8621176PMC
October 2021

Effect of High-Intensity Interval Training on Physical Health in Coronary Artery Disease Patients: A Meta-Analysis of Randomized Controlled Trials.

J Cardiovasc Dev Dis 2021 Nov 18;8(11). Epub 2021 Nov 18.

School of Physical Education, Shandong University, Jinan 250012, China.

The effects of exercise-based cardiac rehabilitation (CR) on physical health in coronary artery disease (CAD) patients has long been established, while the optimal exercise mode remains to be determined. This meta-analysis compared the efficacy of high-intensity interval training (HIIT) versus moderate-intensity continuous training (MICT) in CAD patients. Databases were searched up to December 2020. Twenty-five studies with 1272 participants were analyzed. The results showed that both HIIT and MICT induced significant VO improvement with a 4.52 mL/kg/min ( < 0.01) and 2.36 mL/kg/min ( < 0.01), respectively. Additionally, a larger improvement of VO (1.92 mL/kg/min, < 0.01) was observed in HIIT over MICT. HIIT with medium and long intervals, higher work/rest ratio induced larger VO improvement than the compared subgroup. Interestingly, non-isocaloric exercise protocols induced larger VO improvement compared with isocaloric protocols. In addition, both HIIT and MICT significantly increased anaerobic threshold and peak power with HIIT superior to MICT. No significant different changes were observed in blood pressure after HIIT or MICT intervention, however when HIIT was compared with MICT, MICT seems superior to HIIT in reducing systolic blood pressure (-3.61 mmHg, < 0.01) and diastolic blood pressure (-2.37 mmHg, < 0.01). Although, HIIT and MICT induced significant improvement of most other parameters, like HR, HR, left ventricular ejection fraction (LVEF), quality of life (QoL), no significant differences were noted between groups. This meta-analysis suggested that HIIT is superior to MICT in increasing VO, anaerobic threshold, peak power in CAD patients. Additionally, the efficacy of HIIT over MICT in improving VO was influenced by HIIT intervals, work/rest ratio and total caloric consumption. Both HIIT and MICT did not significantly influence resting BP, however, MICT seemed to be more effective in reducing BP than HIIT. HIIT and MICT equally significantly influenced HR, HR, HRR1min, OUES, LVEF%, QoL.
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http://dx.doi.org/10.3390/jcdd8110158DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8622669PMC
November 2021

YTHDF1 promotes mRNA degradation via YTHDF1-AGO2 interaction and phase separation.

Cell Prolif 2022 Jan 25;55(1):e13157. Epub 2021 Nov 25.

School of Basic Medical Sciences, Wuhan University, Wuhan, China.

Objectives: YTHDF1 is known as a m A reader protein, and many researches of YTHDF1 focused on the regulation of mRNA translation efficiency. However, YTHDF1 is also related to RNA degradation, but how YTHDF1 regulates mRNA degradation is indefinite. Liquid-liquid phase separation (LLPS) underlies the formation of membraneless compartments in mammal cells, and there are few reports focused on the correlation of RNA degradation with LLPS. In this research, we focused on the mechanism of YTHDF1 degraded mRNA through LLPS.

Materials And Methods: The CRISPR/Cas9 knock out system was used to establish the YTHDF1 knock out (YTHDF1-KO) cell lines (HEK293 and HeLa) and METTL14 knock out (METTL14-KO) cell line (HEK293). 4SU-TT-seq was used to check the half-life changes of mRNAs. Actinomycin D and qPCR were used to test the half-life changes of individual mRNA. RNA was stained with SYTO RNA-select dye in wild type (WT) and YTHDF1-KO HeLa cell lines. Co-localization of YTHDF1 and AGO2 was identified by immunofluorescence. The interaction domain of YTHDF1 and AGO2 was identified by western blot. Phase separation of YTHDF1 was performed in vitro and in vivo. Fluorescence recovery after photobleaching (FRAP) was performed on droplets as an assessment of their liquidity.

Results: In this research, we found that deletion of YTHDF1 led to massive RNA patches deposited in cytoplasm. The results of 4SU-TT-seq showed that deletion of YTHDF1 would prolong the half-life of mRNAs. Immunofluorescence data showed that YTHDF1 and AGO2 could co-localize in P-body, and Co-IP results showed that YTHDF1 could interact with AGO2 through YT521-B homology (YTH) domain. We confirmed that YTHDF1 could undergo phase separation in vitro and in vivo, and compared with AGO2, YTHDF1 was more important in P-body formation. The FRAP results showed that liquid AGO2 droplets would convert to gel/solid when YTHDF1 was deleted. As AGO2 plays important roles in miRISCs, we also found that miRNA-mediate mRNA degradation is related to YTHDF1.

Conclusions: YTHDF1 recruits AGO2 through the YTH domain. YTHDF1 degrades targeting mRNAs by promoting P-body formation through LLPS. The deletion of YTHDF1 causes the P-body to change from liquid droplets to gel/solid droplets, and form AGO2/RNA patches, resulting in a degradation delay of mRNAs. These findings reveal a previously unrecognized crosstalk between YTHDF1 and AGO2, raising a new sight of mRNA post-transcriptional regulation by YTHDF1.
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http://dx.doi.org/10.1111/cpr.13157DOI Listing
January 2022

Clinical application of preserving spontaneous breathing non-intubation anesthesia in thoracoscopic surgery for lung cancer under ERAS concept.

Asia Pac J Clin Oncol 2021 Nov 24. Epub 2021 Nov 24.

Department of Thoracic Surgery, the First Affiliated Hospital of Soochow University, Suzhou, China.

Objective: To explore the safety and feasibility of thoracoscopic surgery in patients with lung cancer under non-intubation anesthesia, and to evaluate the advantages of the non-intubation anesthesia compared with intubation anesthesia on enhanced recovery after surgery (ERAS).

Methods: A retrospective cohort study was conducted in which 100 patients who underwent thoracoscopic lung cancer surgery from January 2020 to February 2021 in the Department of Thoracic Surgery of the First Affiliated Hospital of Soochow University were included and divided into non-intubation group (n = 50) and intubation group (n = 50). The primary outcome was the comparison of intra- and postoperative parameters. Secondary outcomes included inflammatory response indicators and intra- and postoperative complications.

Results: There was no significant difference between the two groups in anesthesia effect score, blood loss, lowest pulse oxygen saturation, operation time, postoperative chest tube indwelling time (P > 0.05). Non-intubation group had less intraoperative remifentanil dosage, less change of blood pressure and heart rate, lower postoperative pain numerical score(NRS), less medical costs, smaller incidence rate of throat discomfort (P < 0.05). The non-intubation group was also associated with less extubation time, postanesthesia care unit recovery time, ambulation time, food intake time, postoperative antibiotic use time, and hospital stay (P < 0.05). The increase of C-reactive protein in the non-intubation group was lower than that in the intubation group (P < 0.05).

Conclusion: Non-intubation anesthesia for thoracoscopic lung cancer surgery is safe and feasible. Compared with the intubation anesthesia, it has advantages in ERAS and reducing medical costs.
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http://dx.doi.org/10.1111/ajco.13726DOI Listing
November 2021

Novel Branched-Chain Amino Acid-Catabolism Related Gene Signature for Overall Survival Prediction of Pancreatic Carcinoma.

J Proteome Res 2021 Nov 24. Epub 2021 Nov 24.

Department of Endoscopy, Fudan University Shanghai Cancer Center, Shanghai 200032, China.

Branched-chain amino acid (BCAA) metabolism plays an important role in the pancreatic carcinogenesis, but its mechanism remains unclear. Hence, this study was performed to investigate the value of genes related to BCAA catabolism in pancreatic cancer. The online Gene Expression Omnibus database, The Cancer Genome Atlas, and International Cancer Genome Consortium data sets were searched for bioinformatic analysis. Univariate Cox and Lasso regression were applied to construct a predictive model. Human cancer cell lines and tissue microarray (TMA) were applied for validation. From the 48 BCAA-catabolism enzyme (BCE) genes, a 5-gene risk-score (ABAT, ACAT1, BCAT1, BCAT2, and DBT) was constructed. Patients in high-risk and low-risk groups stratified by risk-score indicated significantly different overall survival. Given the clinical parameters, the risk-score was an independent predictor for prognosis. Among the five genes, BCAT2 and ABAT were hub genes with favorable prognosis value, which was validated by TMA immunohistochemistry (IHC) staining. Immune infiltration analysis indicated high-risk group enriched macrophage, and decreased positive cell density of stromal CD68 macrophage in TMA was observed for BCAT2 with low-expression versus high-expression cases. In conclusion, a risk-score involving five BCE genes was proposed to predict the poor prognosis of pancreatic cancer. On the basis of the immune infiltration analysis, the underlying mechanism might be BCAT2 associated stromal macrophage infiltration.
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http://dx.doi.org/10.1021/acs.jproteome.1c00607DOI Listing
November 2021

Effective light trapping in c-Si thin-film solar cells with a dual-layer split grating.

Appl Opt 2021 Nov;60(33):10312-10321

We propose a dual-layer split nanograting structure in crystalline silicon thin-film solar cells (TFSCs). The split nanograting is designed by introducing two partitioning factors and split times. By employing the finite-difference time-domain method, the light trapping performance and relevant parameters of TFSCs are analyzed and optimized. Numerical computation of optical and electrical simulation shows that the optimal dual-layer split nanograting structure has demonstrated great enhanced light absorption compared with the planar structure. Enhancement of the light trapping effect is associated with light coupling to waveguide modes. The short-circuit current density is reached at 21.66/ with an improvement of 54.6% over the planar structure. All results provide a parting thought for the design of TFSC grating structures.
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http://dx.doi.org/10.1364/AO.443307DOI Listing
November 2021

The Impact on 30-Day Mortality From a Brief Focused Ultrasound-Guided Management Protocol Immediately Before Emergency Noncardiac Surgery in Critically Ill Patients: A Multicenter Randomized Controlled Trial.

J Cardiothorac Vasc Anesth 2021 May 19. Epub 2021 May 19.

Department of Anesthesiology, West China Hospital, Sichuan University, Chengdu, Sichuan, China. Electronic address:

Objective: To determine whether brief ultrasound-guided treatment of hemodynamic shock and respiratory failure immediately before emergency noncardiac surgery reduced 30-day mortality.

Design: Parallel, nonblinded, randomized trial with 1:1 allocation to control and intervention groups.

Setting: Twenty-eight major hospitals within China.

Participants: Six-hundred sixty patients ≥14 years of age, scheduled for emergency noncardiac surgery with evidence of shock (heart rate >120 beat/min, systolic blood pressure< 90 mmHg or requiring inotrope infusion), or respiratory failure (Pulse Oxygen Saturation <92%, respiratory rate >20 beat/min, or requiring mechanical ventilation).

Interventions: A brief (<15 minutes) focused ultrasound of ventricular filling and function, lung, and peritoneal spaces, with predefined treatment recommendation based on the ultrasound was performed before surgery or standard care.

Measurements And Main Results: The primary outcome was 30-day mortality. Secondary outcomes included changes in medical or surgical diagnosis and management due to ultrasound, intensive care unit, and hospital stay and cost, and Short Form-8 quality-of-life scores. Although there were frequent changes in diagnosis (82%) and management (49%) after the ultrasound, mortality at 30 days was not different between groups (50 [15.7%] v 53 [16.3%]; odds ratio 1.05, 0.69-1.6, p = 0.826). There were no differences in the secondary outcomes of the days spent in the hospital (mean 13.8 days, 95% confidence interval [CI] 12.1-15.6 v 14.4 d, 11.8-17.1, p = 0.718) or intensive care unit (mean 9.3 days, 95% CI 7.7-11.0 v 8.7 d, 7.2-10.2, p = 0.562), hospital cost (USD$14.5K, 12.2-16.7 v 13.7, 11.5-15.9, p = 0.611) or Short Form-8 scores at one year (mean 80.9, 95% CI 78.4-83.3 v 79.7, 76.9-82.5, p = 0.54) between participants allocated to the ultrasound and control groups.

Conclusions: In critically ill patients with hemodynamic shock or respiratory failure, a focused ultrasound-guided management did not reduce 30-day mortality but led to frequent changes in diagnosis and patient management.
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http://dx.doi.org/10.1053/j.jvca.2021.05.023DOI Listing
May 2021

Printing dynamic color palettes and layered textures through modeling-guided stacking of electrochromic polymers.

Mater Horiz 2022 Jan 4;9(1):425-432. Epub 2022 Jan 4.

Department of Chemistry, Purdue University, West Lafayette, IN 47907, USA.

In printable electrochromic polymer (ECP) displays, a wide color gamut, precise patterning, and controllable color switching are important. However, it is a significant challenge to achieve such features synergistically. Here, we present a solution-processable ECP stacking scheme, where a crosslinker is co-processed with three primary ECPs (ECP-Cyan, ECP-Magenta, and ECP-Yellow), which endows the primary ECPs with solvent-resistant properties and allows them to be sequentially deposited. varying the film thickness of each ECP layer, a full-color palette can be constructed. The ECP stacking strategy is further integrated with photolithography. Delicate multilayer patterns with overhang and undercut textures can be generated, allowing information displays with spatial dimensionality. In addition, modulating the stacking sequence, the electrochemical onset potentials of the ECP components can be synchronized to reduce unwanted intermediate colors that are often found in co-processed ECPs. Should specific color properties be desired, COMSOL modeling could be applied to guide the stacking. We believe that this ECP stacking strategy opens a new avenue for electrochromic printing and displays.
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http://dx.doi.org/10.1039/d1mh01098kDOI Listing
January 2022

Seismic Response of Resilient Bridges with SMA-Based Rocking ECC-Reinforced Piers.

Materials (Basel) 2021 Oct 29;14(21). Epub 2021 Oct 29.

PowerChina Huadong Engineering Corporation Limited, Hangzhou 311122, China.

Post-earthquake investigation shows that numerous reinforced concrete (RC) bridges were demolished because of large residual displacements. Improving the self-centering capability and hence resilience of these bridges located in earthquake-prone regions is essential. In this regard, a resilient bridge system incorporating engineered cementitious composites (ECC) reinforced piers and shape memory alloy (SMA) energy dissipation components, i.e., SMA washers, is proposed to enhance its resilience when subjected to strong earthquakes. This study commences with a detailed introduction of the resilient SMA-washer-based rocking bridge system with ECC-reinforced piers. Subsequently, a constitutive model of the ECC material is implemented into OpenSees and the constitutive model is validated by test data. The working principle and constitutive model of the SMA washers are also introduced. A series of dynamic analysis on the conventional and resilient rocking bridge systems with ECC-reinforced piers under a suite of ground motions at E1 and E2 earthquake levels are conducted. The analysis results indicate that the resilient rocking bridge system with ECC-reinforced piers has superior resilience and damage control capacities over the conventional one.
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http://dx.doi.org/10.3390/ma14216500DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8585276PMC
October 2021

Relationship between TNF-α levels and psychiatric symptoms in first-episode drug-naïve patients with schizophrenia before and after risperidone treatment and in chronic patients.

BMC Psychiatry 2021 11 11;21(1):561. Epub 2021 Nov 11.

Beijing HuiLongGuan Hospital, Peking University, Beijing, 100096, People's Republic of China.

Background: The influence of antipsychotic drugs on tumor necrosis factor-α (TNF-α) levels is unclear, and there is no consensus on the association between TNF-α and psychotic symptoms. This study aimed to investigate the differences in TNF-α levels and clinical correlations in first-episode drug-naïve (FEDN) patients with schizophrenia before and after treatment and in chronic patients.

Methods: A total of 103 (51 FEDN and 52 chronic) patients and 114 healthy controls were recruited. Demographic and clinical data, including TNF-α levels, were recorded. We used the Positive and Negative Syndrome Scale (PANSS) to measure the psychopathology of all patients.

Results: TNF-α levels before treatment were significantly higher in FEDN patients than in chronic patients and healthy controls. No significant sex differences were found in the TNF-α levels of patients with schizophrenia. The TNF-α levels before treatment were significantly positively related to changes in PANSS negative symptoms in FEDN patients. The TNF-α levels in chronic patients were significantly negatively correlated with the general psychopathology subscales and PANSS total scores.

Conclusions: Increased TNF-α levels in FEDN patients and their correlation with psychopathology indicate that inflammatory cytokines may play a crucial role in the etiopathogenesis of schizophrenia, and inflammation-directed therapy may, therefore, improve negative symptoms.
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http://dx.doi.org/10.1186/s12888-021-03569-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8588730PMC
November 2021
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