Dr. Kathryn J Schunke, PhD - University of Hawaii

Dr. Kathryn J Schunke

PhD

University of Hawaii

Honolulu, HI | United States

Main Specialties: Biology, Cardiovascular Disease

Dr. Kathryn J Schunke, PhD - University of Hawaii

Dr. Kathryn J Schunke

PhD

Introduction

Primary Affiliation: University of Hawaii - Honolulu, HI , United States

Specialties:

Metrics

Number of Publications

13

Publications

Number of Profile Views

290

Profile Views

Number of Article Reads

47

Reads

Number of PubMed Central Citations

94

PubMed Central Citations

Education

Jan 2017
University of Hawaii
Assistant Professor
Jun 2015
Johns Hopkins University
Postdoctoral Fellow
May 2010
Rutgers university
PhD
Mar 2005
University of California Davis
BS

Top co-authors

Gary F Merrill
Gary F Merrill

Rutgers University

5
Roseli Golfetti
Roseli Golfetti

Rutgers University

4
Sunanda S Baliga
Sunanda S Baliga

Rutgers University

4
Nauder Faraday
Nauder Faraday

Johns Hopkins University School of Medicine

3
Norell M Hadzimichalis
Norell M Hadzimichalis

Rutgers University

3
Kathryn M Jaques-Robinson
Kathryn M Jaques-Robinson

University of Hawaii

3
Jian Zhang
Jian Zhang

Biomedical Polymers Laboratory

2
Ralph V Shohet
Ralph V Shohet

John A. Burns School of Medicine

2
Jiadi Xu
Jiadi Xu

University of Michigan

2

Publications

13Publications

47Reads

94PubMed Central Citations

Protein Kinase C Binding Protein 1(PRKCBP1) Inhibits Hypoxia Inducible Factor 1 (HIF-1) in the Heart.

Cardiovasc Res 2018 Nov 5. Epub 2018 Nov 5.

From the Center for Cardiovascular Research, Department of Medicine, John A. Burns School of Medicine, University of Hawaii, Honolulu, HI.

View Article
November 2018
2 Reads
5.94 Impact Factor

Protein Kinase C Binding Protein 1(PRKCBP1) Inhibits Hypoxia Inducible Factor 1 (HIF-1) in the Heart

Cardiovasc Res. 2018 Nov 5. doi: 10.1093/cvr/cvy278. [Epub ahead of print]

Cardiovascular Research

Aims: Hypoxia-inducible factor 1 alpha (HIF-1α) is a key transcription factor responsible for the induction of genes that facilitate adaptation to hypoxia. To study HIF-1 signaling in the heart, we developed a mouse model in which an oxygen-stable form of HIF-1α can be inducibly expressed in cardiac myocytes, under the regulation of tetracycline. Methods & Results: Remarkably, expression of the transgene in mice generated two distinct phenotypes. One was the expected expression of HIF-regulated transcripts and associated changes in cardiac angiogenesis and contractility. The other was an "unresponsive phenotype" with much less expression of typical HIF-response genes and substantial expression of a zinc-finger protein, Protein Kinase C Binding Protein 1 (PRKCBP1). We have demonstrated that this second phenotype is due to an insertion of a fragment of DNA upstream of the PRKCBP1 gene that contains two additional canonical HIF binding sites and leads to substantial HIF binding, assessed by ChIP, and transcriptional activation. This insertion is found only in the FVB strain of mice that contributed the αMHC-tet binding protein transgene to these biallelic mice. In HEK293 cells transfected with oxygen-stable HIF-1α and PRKCBP1 we demonstrated inhibition of HIF-1 activity by a luciferase reporter assay. Using mouse primary cells and cell lines we show that transfection with oxygen-stable HIF-1α and PRKCBP1 reduced expression of direct HIF-1 gene targets and that knockdown of PRKCBP1 removes that negative inhibition. Consistent with previous reports suggesting that PRKCBP1 modulates the chromatin landscape, we found that HL-1 cells transfected with oxygen-stable HIF-1α and PRKCBP1 have reduced global 5-methyl cytosine compared to HIF-1 alone. Conclusions: We show genetic, transcriptional, biochemical, and physiological evidence that PRKCBP1 inhibits HIF activity. Identification of a new oxygen-dependent and previously unsuspected regulator of HIF may provide a target for new therapeutic approaches to ischemic heart disease.

View Article
November 2018
21 Reads

Separating fast and slow exchange transfer and magnetization transfer using off-resonance variable-delay multiple-pulse (VDMP) MRI.

Magn Reson Med 2018 Oct 5;80(4):1568-1576. Epub 2018 Feb 5.

Russell H. Morgan Department of Radiology and Radiological Science, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.

View Article
October 2018
2 Reads
2 PubMed Central Citations(source)
3.57 Impact Factor

HIF1 mediates a switch in pyruvate kinase isoforms after myocardial infarction.

Physiol Genomics 2018 Jul 13;50(7):479-494. Epub 2018 Apr 13.

Center for Cardiovascular Research, John A. Burns School of Medicine, University of Hawaii , Honolulu, Hawaii.

View Article
July 2018
1 Read
1 PubMed Central Citation(source)
2.37 Impact Factor

A novel atherothrombotic model of ischemic stroke induced by injection of collagen into the cerebral vasculature.

J Neurosci Methods 2015 Jan 12;239:65-74. Epub 2014 Oct 12.

Department of Anesthesiology/Critical Care Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA. Electronic address:

View Article
January 2015
7 Reads
2 PubMed Central Citations(source)
2.02 Impact Factor

Cathepsin G-dependent modulation of platelet thrombus formation in vivo by blood neutrophils.

PLoS One 2013 5;8(8):e71447. Epub 2013 Aug 5.

Department of Anesthesiology/Critical Care Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America.

View Article
April 2014
1 Read
13 PubMed Central Citations(source)
3.23 Impact Factor

Acetaminophen attenuates doxorubicin-induced cardiac fibrosis via osteopontin and GATA4 regulation: reduction of oxidant levels.

J Cell Physiol 2013 Oct;228(10):2006-14

Department of Cell Biology and Neuroscience, Rutgers University, Piscataway, NJ, USA.

View Article
October 2013
7 Reads
5 PubMed Central Citations(source)
3.84 Impact Factor

Acetaminophen reduces mitochondrial dysfunction during early cerebral postischemic reperfusion in rats.

Brain Res 2010 Mar 14;1319:142-54. Epub 2010 Jan 14.

Department of Cell Biology and Neuroscience, Rutgers University, 604 Allison Road, Piscataway, New Jersey 08854, USA.

View Article
March 2010
6 Reads
8 PubMed Central Citations(source)
2.84 Impact Factor

Mechanisms of enhanced beta-adrenergic reserve from cardiac resynchronization therapy.

Circulation 2009 Mar 23;119(9):1231-40. Epub 2009 Feb 23.

Department of Medicine, Johns Hopkins Medical Institutions, Baltimore, MD, USA.

View Article
March 2009
6 Reads
36 PubMed Central Citations(source)
14.43 Impact Factor

Acetaminophen is cardioprotective against H2O2-induced injury in vivo.

Exp Biol Med (Maywood) 2008 Oct 14;233(10):1315-22. Epub 2008 Aug 14.

Department of Cell Biology and Neuroscience, Rutgers University, Piscataway, NJ 08854, USA.

View Article
October 2008
5 Reads
10 PubMed Central Citations(source)

Acetaminophen-mediated cardioprotection via inhibition of the mitochondrial permeability transition pore-induced apoptotic pathway.

Am J Physiol Heart Circ Physiol 2007 Dec 5;293(6):H3348-55. Epub 2007 Oct 5.

Department of Cell Biology and Neuroscience, Division of Life Sciences, Rutgers University, 604 Allison Road, Piscataway, NJ 08854, USA.

View Article
December 2007
5 Reads
13 PubMed Central Citations(source)
3.84 Impact Factor

Antiarrhythmic properties of acetaminophen in the dog.

Exp Biol Med (Maywood) 2007 Oct;232(9):1245-52

Division of Life Sciences, Department of Cell Biology and Neurosciences, Rutgers University, Piscataway, New Jersey 08854, USA.

View Article
October 2007
5 Reads
4 PubMed Central Citations(source)

Top co-authors

Gary F Merrill
Gary F Merrill

Rutgers University

5
Roseli Golfetti
Roseli Golfetti

Rutgers University

4
Sunanda S Baliga
Sunanda S Baliga

Rutgers University

4
Nauder Faraday
Nauder Faraday

Johns Hopkins University School of Medicine

3
Norell M Hadzimichalis
Norell M Hadzimichalis

Rutgers University

3
Kathryn M Jaques-Robinson
Kathryn M Jaques-Robinson

University of Hawaii

3
Jian Zhang
Jian Zhang

Biomedical Polymers Laboratory

2
Ralph V Shohet
Ralph V Shohet

John A. Burns School of Medicine

2
Jiadi Xu
Jiadi Xu

University of Michigan

2