Publications by authors named "Kateřina Mamulová Kutláková"

8 Publications

  • Page 1 of 1

Preparation of highly wettable coatings on Ti-6Al-4V ELI alloy for traumatological implants using micro-arc oxidation in an alkaline electrolyte.

Sci Rep 2020 11 13;10(1):19780. Epub 2020 Nov 13.

Institute of Physiology of the Czech Academy of Sciences, v.v.i., Videnska 1083, 142 20, Prague 4, Czech Republic.

Pulsed micro-arc oxidation (MAO) in a strongly alkaline electrolyte (pH > 13), consisting of NaSiO⋅9HO and NaOH, was used to form a thin porous oxide coating consisting of two layers differing in chemical and phase composition. The unique procedure, combining MAO and removal of the outer layer by blasting, enables to prepare a coating suitable for application in temporary traumatological implants. A bilayer formed in an alkaline electrolyte environment during the application of MAO enables the formation of a wear-resistant layer with silicon incorporated in the oxide phase. Following the removal of the outer rutile-containing porous layer, the required coating properties for traumatological applications were determined. The prepared surfaces were characterized by scanning electron microscopy, X-ray diffraction patterns, X-ray photoelectron spectroscopy, atomic force microscopy and contact angle measurements. Cytocompatibility was evaluated using human osteoblast-like Saos-2 cells. The newly-developed surface modifications of Ti-6Al-4V ELI alloy performed satisfactorily in all cellular tests in comparison with MAO-untreated alloy and standard tissue culture plastic. High cell viability was supported, but the modifications allowed only relatively slow cell proliferation, and showed only moderate osseointegration potential without significant support for matrix mineralization. Materials with these properties are promising for utilization in temporary traumatological implants.
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http://dx.doi.org/10.1038/s41598-020-76448-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7666130PMC
November 2020

Phytotoxicity of ZnO/kaolinite nanocomposite-is anchoring the right way to lower environmental risk?

Environ Sci Pollut Res Int 2019 Jul 30;26(21):22069-22081. Epub 2019 May 30.

Institute of Experimental Botany AS CR, Rozvojová 313, 165 02, Prague, Czech Republic.

The importance of studies on photoactive zinc oxide nanoparticles (ZnO NPs) increases with increasing environmental pollution. Since the ZnO NPs (and NPs in general) also pose an environmental risk, and since an understanding of the risk is still not sufficient, it is important to prevent their spread into the environment. Anchoring on phyllosilicate particles of micrometric size is considered to be a useful way to address this problem, however, so far mainly on the basis of leaching tests in pure water. In the present study, the phytotoxicity of kaolinite/ZnO NP (10, 30, and 50 wt.%) nanocomposites in concentrations 10, 100, and 1000 mg/dm tested on white mustard (Sinapis alba) seedlings was found to be higher (relative lengths of roots are ~ 1.4 times lower) compared with seedlings treated with pristine ZnO NPs. The amount of Zn accumulated from the nanocomposites in white mustard tissues was ~ 2 times higher than can be expected based on the ZnO content in the nanocomposites compared with the ZnO content (100 wt.%) in pristine ZnO NPs. For the false fox-sedge (Carex otrubae) plants, the amount of Zn accumulated in roots and leaves was ~ 2.25 times higher and ~ 2.85 times higher, respectively, compared with that of the pristine ZnO NPs (with respect to the ZnO content). Increased phytotoxicity of the nanocomposites and higher uptake of Zn by plants from the nanocomposites in comparison with pristine ZnO NPs suggest that the immobilization of ZnO NPs on the kaolinite does not reduce the environmental risk.
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http://dx.doi.org/10.1007/s11356-019-05529-9DOI Listing
July 2019

Ti and Zn Content in Moss Shoots After Exposure to TiO and ZnO Nanoparticles: Biomonitoring Possibilities.

Bull Environ Contam Toxicol 2019 Feb 17;102(2):218-223. Epub 2019 Jan 17.

Nanotechnology Centre, VŠB-Technical University of Ostrava, 17. listopadu 15/2172, 708 33, Ostrava, Czech Republic.

To assess the uptake of nanoparticles by moss shoots and the possibility of biomonitoring the moss of nanoparticle pollution, two moss species frequently used in biomonitoring surveys [Hylocomium splendens (Hedw.) Schimp. and Pleurozium schreberi (Brid.) Mitt.] were repeatedly exposed to known concentrations of either nano-TiO or nano-ZnO suspensions. The interspecies differences were assessed by exposing both the species to 1 g L nano-ZnO suspension, H. splendens samples were also exposed to either 0.1 g L or 1 g L suspension of nano TiO. The exposed samples were analysed for Zn or Ti content using Inductively Coupled Plasma-Atomic Emission Spectroscopy. Both species showed a similar accumulation pattern, H. splendens being a slightly better accumulator. The washing suggests that Ti successfully penetrated the interior of the gametophyte. Since the relationship between the exposure and accumulation is linear, moss biomonitoring is, hereby, considered to be a viable, novel technique in nanoparticle pollution assessment.
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http://dx.doi.org/10.1007/s00128-019-02544-xDOI Listing
February 2019

Effects of binder choice in converter and blast furnace sludge briquette preparation: Environmental and practical implications.

Waste Manag 2018 Sep 17;79:30-37. Epub 2018 Jul 17.

Nanotechnology Centre, VŠB-Technical University of Ostrava, 17. listopadu 15/2172, 708 33 Ostrava-Poruba, Czech Republic.

Blast furnace and converter sludges are fine-grained waste materials characterized as dangerous waste with a negative impact on the environment. One way of recycling of such materials is briquetting followed by reuse of the material in the blast furnace. In the briquetting process, an important step is the choice of the binder suitable for manufacturing the briquettes with suitable mechanical properties. In this work, the effect of the binder choice (laundry starch UNIPRET, Portland cement) on the reduction of iron oxides in the assessed waste materials during thermal treatment (900, 1000, 1100 °C) is evaluated. Simultaneously, the effect of the binder choice on the amount and composition of the resulting waste gas was evaluated as well as its possible impact on the environment. The performed experiments proved the mutual relationship between the level of iron oxides to metal iron conversion, the binder content and retention temperature. Type of binder also affected the volume of the resulting waste gas. Factor analysis for mixed data (FAMD) proved that the resulting concentrations of the assessed hydrocarbons were correlated (apart from ethyne) and that they are closely associated with the binder applied. Conversely, the concentrations of ethyne, carbon monoxide and carbon dioxide were not associated with the binder but with the retention temperature. FAMD did not show any direct effect of final retention temperature on the amount of the rest of the resulting hydrocarbons. In comparison with the starch-containing briquettes, the cement-containing briquettes were also proved to lead to lower resulting concentrations of PAHs in the waste gas.
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http://dx.doi.org/10.1016/j.wasman.2018.06.051DOI Listing
September 2018

Recycling of blast furnace sludge by briquetting with starch binder: Waste gas from thermal treatment utilizable as a fuel.

Waste Manag 2016 Feb 9;48:471-477. Epub 2015 Dec 9.

VŠB-Technical University of Ostrava, Nanotechnology Centre, 17. listopadu 15/2172, 708 33 Ostrava-Poruba, Czech Republic.

Steel plants generate significant amounts of wastes such as sludge, slag, and dust. Blast furnace sludge is a fine-grained waste characterized as hazardous and affecting the environment negatively. Briquetting is one of the possible ways of recycling of this waste while the formed briquettes serve as a feed material to the blast furnace. Several binders, both organic and inorganic, had been assessed, however, only the solid product had been analysed. The aim of this study was to assess the possibilities of briquetting using commonly available laundry starch as a binder while evaluating the possible utilization of the waste gas originating from the thermal treatment of the briquettes. Briquettes (100g) were formed with the admixture of starch (UNIPRET) and their mechanical properties were analysed. Consequently, they were subjected to thermal treatment of 900, 1000 and 1100°C with retention period of 40min during which was the waste gas collected and its content analysed using gas chromatography. Dependency of the concentration of the compounds forming the waste gas on the temperature used was determined using Principal component analysis (PCA) and correlation matrix. Starch was found to be a very good binder and reduction agent, it was confirmed that metallic iron was formed during the thermal treatment. Approximately 20l of waste gas was obtained from the treatment of one briquette; main compounds were methane and hydrogen rendering the waste gas utilizable as a fuel while the greatest yield was during the lowest temperatures. Preparation of blast furnace sludge briquettes using starch as a binder and their thermal treatment represents a suitable method for recycling of this type of metallurgical waste. Moreover, the composition of the resulting gas is favourable for its use as a fuel.
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http://dx.doi.org/10.1016/j.wasman.2015.11.047DOI Listing
February 2016

Iron-based granules in body of bumblebees.

Biometals 2015 Feb 28;28(1):89-99. Epub 2014 Oct 28.

IT4Innovations Centre and Institute of Physics, VSB - Technical University of Ostrava, 17. listopadu 15/2172, 708 33, Ostrava, Czech Republic,

The paper deals with the presence of iron-based granules in body parts of bumblebees. Two groups of bumblebees were collected from their natural habitat, industrial landscape, and from a breeding station. Detection of the magnetic particles was performed by a vibratory magnetometer and their morphology and elemental composition was analysed by scanning electron microscopy with EDX microanalysis. By means of the EDX spectra, wild bumblebees were found to have many magnetic and non-magnetic particles on their body, containing Fe, O, Al, Si, Bi, Mg, K, and Ni, likely having origin in the industrial pollution of the environment. In the case of bred bumblebees the presence of iron-rich granules, which occurred more abundantly in subsurface tissues on the head and wings, was observed. Phase analysis based on X-ray diffraction shows that iron-based granules contain magnetite and wuestite and Mössbauer spectroscopy admits a superparamagnetic form of these minerals. Magnetoreception, i.e. the sensory function of these granules, is discussed within the paper.
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http://dx.doi.org/10.1007/s10534-014-9805-9DOI Listing
February 2015

Antibacterial activity of kaolinite/nanoTiO2 composites in relation to irradiation time.

J Photochem Photobiol B 2014 Jun 18;135:17-22. Epub 2014 Apr 18.

Nanotechnology Centre, VŠB - Technical University of Ostrava, 17. listopadu 15, 708 33 Ostrava, Poruba, Czech Republic.

The paper addresses laboratory preparation and antibacterial activity testing of kaolinite/nanoTiO2 composite in respect of the daylight irradiation time. Kaolinite/nanoTiO2 composites with 20 and 40 wt% of TiO2 were laboratory prepared, dried at 105 °C and calcined at 600 °C. The calcination caused transformation of kaolinite to metakaolinite and origination of the metakaolinite/nanoTiO2 composite. X-ray powder diffraction, Raman and FTIR spectroscopic methods revealed titanium dioxide only in the form of anatase in all evaluated samples (non-calcined and calcined) and also transformation of kaolinite to metakaolinite after the calcination treatment. Scanning electron microscopy was used as a method for characterization of morphology and elemental composition of the studied samples. A standard microdilution test was used to determine the antibacterial activity using four human pathogenic bacterial strains (Staphylococcus aureus, Escherichia coli, Enterococcus faecalis, Pseudomonas aeruginosa). A lamp with a wide spectrum bulb simulating daylight was used for induction of photocatalysis. The antibacterial assays found all the KATI samples to have antibacterial potency with different onset of the activity when calcined samples exhibited antibacterial activity earlier than the non-calcined. Significant difference in antibacterial activity of KATI samples for different bacterial strains was not observed.
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http://dx.doi.org/10.1016/j.jphotobiol.2014.04.004DOI Listing
June 2014

Modified clay minerals efficiency against chemical and biological warfare agents for civil human protection.

J Hazard Mater 2014 Apr 12;271:65-72. Epub 2014 Feb 12.

Nanotechnology Centre, VŠB-Technical University of Ostrava, 17. listopadu 15, Poruba, Ostrava 708 33, Czech Republic. Electronic address:

Sorption efficiencies of modified montmorillonite and vermiculite of their mono ionic Na and organic HDTMA and HDP forms were studied against chemical and biological warfare agents such as yperite and selected bacterial strains. Yperite interactions with modified clay minerals were observed through its capture in low-density polyethylene foil-modified clay composites by measuring yperite gas permeation with using chemical indication and gas chromatography methods. The antibacterial activities of synthetized organoclays were tested against selected Gram-positive and Gram-negative bacterial species in minimum inhibitory concentration tests. The obtained results showed a positive influence of modified clay minerals on the significant yperite breakthrough-time increase. The most effective material was the polyethylene-Na form montmorillonite, while the polyethylene-Na form vermiculite showed the lowest efficiency. With increasing organic cations loading in the interlayer space the montmorillonite efficiency decreased, and in the case of vermiculite an opposite effect was observed. Generally the modified montmorillonites were more effective than modified vermiculites. The HDP cations seem to be more effective compare to the HDTMA. The antibacterial activity tests confirmed efficiency of all organically modified clay minerals against Gram-positive bacteria. The confirmation of antibacterial activity against Y. pestis, plague bacteria, is the most interesting result of this part of the study.
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http://dx.doi.org/10.1016/j.jhazmat.2014.01.059DOI Listing
April 2014