Publications by authors named "Katarzyna Wiglusz"

11 Publications

  • Page 1 of 1

In Vitro Assessment of Long-Term Fluoride Ion Release from Nanofluorapatite.

Materials (Basel) 2021 Jul 4;14(13). Epub 2021 Jul 4.

Institute of Low Temperature and Structure Research, Polish Academy of Sciences, Okolna 2, 50-422 Wroclaw, Poland.

The issue concerning the tooth decay is ongoing, therefore the study of materials with potential use in its prevention is crucial. This study aimed to analyze the long-term release of fluoride from synthesized nanofluorapatite in various in vitro environments for its potential use in dental materials. We placed 100 mg samples in 0.9% NaCl or deionized water and incubated them at 37 °C or 22 °C for 12 weeks. F- levels were read at 1, 3, 24, 48, 72, and 96 h, and thereafter weekly. The levels of F- released at specific time intervals, as well as their cumulative values were compared. In a solution of 0.9% NaCl at 22 °C, there were no significant differences in the amount of F- released in the assessed time intervals, while at 37 °C, the highest value was read after 24 h (0.0697 ppm + 0.0006; < 0.05). In deionized water, the highest amount of F- at 22 °C was read after 4 weeks (0.0776 ppm + 0.0028; < 0.05), and at 37 °C, it was also the highest after 4 weeks (0.0910 ppm + 0.0156; < 0.05). Under the same conditions, after 5 weeks the cumulative level of F- released (0.6216 ppm + 0.0085) significantly increased ( < 0.05), when compared to the samples placed in 0.9% NaCl at 37 °C and 22 °C (0.5493 ppm + 0.0321 and 0.5376 ppm + 0.0234, respectively). FAp releases F- for a long period of time in all assessed environments, therefore it is advised to continue testing in vivo models. Due to the probable remineralization effect towards hard tooth tissues, fluorapatite can be used in the prevention and treatment of dental caries and dentin hypersensitivity.
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http://dx.doi.org/10.3390/ma14133747DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8269907PMC
July 2021

How Does Glycation Affect Binding Parameters of the Albumin-Gliclazide System in the Presence of Drugs Commonly Used in Diabetes? In Vitro Spectroscopic Study.

Molecules 2021 Jun 24;26(13). Epub 2021 Jun 24.

Department of Toxicology, Wroclaw Medical University, Borowska 211, PL-50556 Wrocław, Poland.

In this research, the selected drugs commonly used in diabetes and its comorbidities (gliclazide, cilazapril, atorvastatin, and acetylsalicylic acid) were studied for their interactions with bovine serum albumin-native and glycated. Two different spectroscopic methods, fluorescence quenching and circular dichroism, were utilized to elucidate the binding interactions of the investigational drugs. The glycation process was induced in BSA by glucose and was confirmed by the presence of advanced glycosylation end products (AGEs). The interaction between albumin and gliclazide, with the presence of another drug, was confirmed by calculation of association constants (0.11-1.07 × 10 M). The nature of changes in the secondary structure of a protein depends on the drug used and the degree of glycation. Therefore, these interactions may have an influence on pharmacokinetic parameters.
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http://dx.doi.org/10.3390/molecules26133869DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8270297PMC
June 2021

Study of Flebogrif-A New Tool for Mechanical Sclerotherapy-Effectiveness Assessment Based on Animal Model.

Nanomaterials (Basel) 2021 Feb 21;11(2). Epub 2021 Feb 21.

Institute of Low Temperature and Structure Research, PAS, Okolna 2, 50-422 Wroclaw, Poland.

Sclerotherapy is the chemical occlusion of vessels using an intravenous injection of a liquid or foamed sclerosing agent that is used in the therapy of blood and lymphatic vessels malformations in the young, and for spider veins, smaller varicose veins, hemorrhoids and hydroceles in adults. This study aimed to assess the effectiveness of mechanosclerotherapy of venous veins with a new device-Flebogrif-based on an animal model. The experiment was performed on nine Polish Merino sheep weighing 40-50 kilograms. The animals were anesthetized intravenously. The material was divided into three groups: two experimental (1 and 2) and control (3) group. The first experimental group was treated with the use of Flebogrif and a sclerosant simultaneously, while only Flebogrif was used in the second experimental group. Flebogrif was applied into the lateral saphenous vein of both pelvic limbs. The vessel wall thickness was estimated at four points of the histological image in mm (V1, V2, V3, V4). For one month, the animals were euthanized, and the occlusion rate of the treated veins and changes in the vein wall were determined. Histological slides were analyzed under a light microscope and histometry of the vein wall was performed. The Shapiro-Wilk test and the quantity of the investigated parameter groups allowed for using a non-parametric method at four points to compare thickness measurements (the Mann-Whitney test), with < 0.05. The Mann-Whitney test indicated statistically significant differences between both experimental groups. The results obtained from morphometrical and histological analysis showed better results in the first experimental group than those of the second experimental group. Finally, statistical analysis revealed significant differences between the both the experimental group and control group in morphological analysis. The achieved results allowed us to conclude that the simultaneous use of Flebogrif and a sclerosant yielded better results of vein lumen reduction than the use of Flebogrif alone.
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http://dx.doi.org/10.3390/nano11020544DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7924836PMC
February 2021

Application of Selected Nanomaterials and Ozone in Modern Clinical Dentistry.

Nanomaterials (Basel) 2021 Jan 20;11(2). Epub 2021 Jan 20.

Institute of Low Temperature and Structure Research, Polish Academy of Sciences, Okólna 2, 50-422 Wrocław, Poland.

This review is an attempt to summarize current research on ozone, titanium dioxide (TiO), silver (Ag), copper oxide CuO and platinum (Pt) nanoparticles (NPs). These agents can be used in various fields of dentistry such as conservative dentistry, endodontic, prosthetic or dental surgery. Nanotechnology and ozone can facilitate the dentist's work by providing antimicrobial properties to dental materials or ensuring a decontaminated work area. However, the high potential of these agents for use in medicine should be confirmed in further research due to possible side effects, especially in long duration of observation so that the best way to apply them can be obtained.
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http://dx.doi.org/10.3390/nano11020259DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7909445PMC
January 2021

The Influence of Ozonated Olive Oil-Loaded and Copper-Doped Nanohydroxyapatites on Planktonic Forms of Microorganisms.

Nanomaterials (Basel) 2020 Oct 10;10(10). Epub 2020 Oct 10.

Institute of Low Temperature and Structure Research, Polish Academy of Sciences, Okolna 2, 50-422 Wroclaw, Poland.

The research has been carried out with a focus on the assessment of the antimicrobial efficacy of pure nanohydroxyapatite, Cu-doped nanohydroxyapatite, ozonated olive oil-loaded nanohydroxyapatite, and Cu-doped nanohydroxyapatite, respectively. Their potential antimicrobial activity was investigated against , and . Among all tested materials, the highest efficacy was observed in terms of ozonated olive oil. The studies were performed using an Ultraviolet-Visible spectrophotometry (UV-Vis), electron microscopy, and statistical methods, by determining the value of Colony-Forming Units (CFU/mL) and Minimal Inhibitory Concentration (MIC).
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http://dx.doi.org/10.3390/nano10101997DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7650683PMC
October 2020

Removal of Composite Restoration from the Root Surface in the Cervical Region Using Er: YAG Laser and Drill-In Vitro Study.

Materials (Basel) 2020 Jul 7;13(13). Epub 2020 Jul 7.

Institute of Low Temperature and Structure Research, Polish Academy of Sciences, Okolna 2, 50-422 Wroclaw, Poland.

Background: Recently, the defects of the tooth surface in the cervical region are often restored using composite filling materials. It should meet the needs of the patients regarding esthetics and material stability. The aim of the study was to analyze the tooth root surface at the cervical region after the removal of the composite filling material by means of the Erbium-doped Yttrium Aluminium Garnet (Er: YAG) laser or drill using the scanning electron microscopy (SEM) and fluorescence microscopy.

Materials And Methods: For the purposes of this study, 14 premolar teeth ( = 14) were removed due to orthodontic reasons. The rectangular shape cavities with 3 mm in width and 1.5 mm in height were prepared with a 0.8 mm bur on high-speed contra-angle in the tooth surface just below cemento-enamel junction (CEJ) and filled with the composite material. The composite material was removed with the Er: YAG laser at a power of 3.4 W, energy 170 mJ, frequency 20 Hz, pulse duration 300 μs, tip diameter 0.8 mm, air/fluid cooling 3 mL/s, and time of irradiation: 6 sec, at a distance from teeth of 2 mm (G1 group, = 7) or a high-speed contra-angle bur (G2 group, = 7). After the removal of composite material, the surfaces of teeth were examined using the scanning electron microscopy (SEM) and fluorescence microscopy.

Results: The Er: YAG irradiation allowed to remove completely the composite material from the tooth cavity. The study confirmed, that the ends of collagen fibers were only partially denatured after the Er: YAG laser application.

Conclusion: It has been proved that using the Er: YAG laser is an effective and safe method of composite removal for the dentin surface.
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http://dx.doi.org/10.3390/ma13133027DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7372447PMC
July 2020

The interaction between human serum albumin and antidiabetic agent - exenatide: determination of the mechanism binding and effect on the protein conformation by fluorescence and circular dichroism techniques - Part I.

J Biomol Struct Dyn 2020 May 22;38(8):2267-2275. Epub 2019 Jun 22.

Department of Analytical Chemistry, Faculty of Pharmacy, Wrocław Medical University, Wrocław, Poland.

The interactions between transport proteins and drugs are very important from the pharmacological point of view. In this study, using fluorescence and circular dichroism (CD) techniques, we investigated the interaction between human serum albumin (HSA) and incretin antidiabetic drug - exenatide. Moreover, the effect of common metal ions (Ca, Zn, Cr) on the exenatide-HSA binding - was also described. Based on the experimental data under pseudophysiological conditions, the calculated binding constant values are on the order of 10 M, and the constants are lower in the presence of metal ions. We observed the increase of the hydrophobicity near the tryptophan-214 residue in subdomain IIA, but almost no change in the hydrophobicity surrounding tyrosine residues. A similar effect on the tryptophan microenvironment is influenced by metal ions. The calculated thermodynamic parameters indicated that the characteristic electrostatic and hydrophobic interactions play an important role in the albumin-exenatide complexes. The CD studies showed that exenatide does not change the secondary structure of the protein but used metal ions have some impact on albumin α-helical content.Communicated by Ramaswamy H. Sarma.
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http://dx.doi.org/10.1080/07391102.2019.1630007DOI Listing
May 2020

Multispectroscopic studies of the interaction of folic acid with glycated human serum albumin.

J Biomol Struct Dyn 2019 09 17;37(14):3731-3739. Epub 2018 Nov 17.

a Department of Analytical Chemistry, Faculty of Pharmacy , Wroclaw Medical University , Wrocław , Poland.

The interaction between glycated human serum albumin (gHSA) and folic acid (FA) was investigated by various spectroscopic techniques, such as fluorescence, circular dichroism, UV-vis absorption spectroscopy and electrophoretic light scattering technique. These methods characterize the binding properties of an albumin-folic acid system. The binding constants values () at 300 and 310 K are about 10 M. The standard enthalpy change (Δ) and the standard entropy change (Δ) were calculated to be ∼-20 kJ mol and ∼16 J mol K, respectively, which indicate characteristic electrostatic interactions between gHSA and folic acid. The CD studies showed that there are no significant conformational changes in the secondary structure of the protein. Moreover, the zeta potential measurements proved that under physiological conditions the gHSA-folic acid complex shows instability. No significant changes in the secondary structure of the protein and reversible drug binding are the desirable effect from pharmacological point of view. Communicated by Ramaswamy H. Sarma.
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http://dx.doi.org/10.1080/07391102.2018.1526713DOI Listing
September 2019

The effect of glycation on bovine serum albumin conformation and ligand binding properties with regard to gliclazide.

Spectrochim Acta A Mol Biomol Spectrosc 2018 Jan 6;189:625-633. Epub 2017 Sep 6.

Department of Toxicology, Faculty of Pharmacy, Wroclaw Medical University, Borowska 211, 50-556 Wroclaw, Poland. Electronic address:

Albumin, the major serum protein, plays a variety of functions, including binding and transporting endogenous and exogenous ligands. Its molecular structure is sensitive to different environmental modifiers, among which glucose is one of the most significant. In vivo albumin glycation occurs under physiological conditions, but it is increased in diabetes. Since bovine serum albumin (BSA) may serve as a model protein in in vitro experiments, we aimed to investigate the impact of glucose-mediated BSA glycation on the binding capacity towards gliclazide, as well as the ability of this drug to prevent glycation of the BSA molecule. To reflect normo- and hyperglycemia, the conditions of the glycation process were established. Structural changes of albumin after interaction with gliclazide (0-14μM) were determined using fluorescence quenching and circular dichroism spectroscopy. Moreover, thermodynamic parameters as well as energy transfer parameters were determined. Calculated Stern-Volmer quenching constants, as well as binding constants for the BSA-gliclazide complex, were lower for the glycated form of albumin than for the unmodified protein. The largest, over 2-fold, decrease in values of binding parameters was observed for the sample with 30mM of glucose, reflecting the poorly controlled diabetic state, which indicates that the degree of glycation had a critical influence on binding with gliclazide. In contrast to significant changes in the tertiary structure of BSA upon binding with gliclazide, only slight changes in the secondary structure were observed, which was reflected by about a 3% decrease of the α-helix content of glycated BSA (regardless of glucose concentration) in comparison to unmodified BSA. The presence of gliclazide during glycation did not affect its progress. The results of this study indicate that glycation significantly changed the binding ability of BSA towards gliclazide and the scale of these changes depended on glucose concentration. It may have a direct impact on the free drug fraction and its pharmacokinetic behavior, including the risk of hypoglycemic episodes or unexpected interactions with other ligands. The use of BSA in examining binding effects upon glycation seems to be good model for preliminary research and may be used to identify a potential drug response in a diabetic state.
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http://dx.doi.org/10.1016/j.saa.2017.08.071DOI Listing
January 2018

Li activated nanohydroxyapatite doped with Eu ions enhances proliferative activity and viability of human stem progenitor cells of adipose tissue and olfactory ensheathing cells. Further perspective of nHAP:Li, Eu application in theranostics.

Mater Sci Eng C Mater Biol Appl 2017 Sep 7;78:151-162. Epub 2017 Apr 7.

Institute of Low Temperature and Structure Research, Polish Academy of Sciences, Okolna 2, 50-422 Wroclaw, Poland; Centre for Advanced Materials and Smart Structures, Polish Academy of Sciences, Okolna 2, 50-950 Wroclaw, Poland. Electronic address:

Spinal cord injuries (SCI) often require simultaneous regeneration of nerve tissue and bone. Hydroxyapatites are described as bioresorbable materials with proper biocompatibility and osteoconductivity, therefore its application for spinal surgery is considered. In this paper, we present repeatable method for developing nanocrystalline calcium hydroxyapatites structurally modified with Li ions (nHAP:Li). Obtained biomaterials were profoundly characterized in terms of their physicochemical properties. Moreover, we have shown that nHAP:Li doped with europium (Eu) may serve as a theranostic agent, what additionally extend its potential usage for SCI treatment. The biocompatibility of nHAP:Li was determined using human olfactory ensheathing cells (hOECs) and adipose tissue-derived multipotent stromal cells (hASCs). Both population of cells are eagerly applied for cell-based therapies in SCI, mainly due to their paracrine activity. The extensive in vitro studies showed that nHAP:Li promotes the cells proliferation, viability and cell-cell interactions. Obtained results provides encouraging approach that may have potential application in regenerative medicine and that could fulfil the promise of personalized medicine - important in SCI treatment.
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http://dx.doi.org/10.1016/j.msec.2017.04.041DOI Listing
September 2017

Interactions of human serum albumin with meloxicam: characterization of binding site.

J Pharm Biomed Anal 2010 Jun 4;52(2):300-4. Epub 2010 Jan 4.

Department of Analytical Chemistry, Faculty of Pharmacy, Wrocław Medical University, Szewska 38, 50-139 Wrocław, Poland.

Human serum albumin (HSA) is the most prominent protein in plasma. The three-domain design of HSA provides a variety of binding sites for many ligands, including heme, bilirubin and drugs. Here, we report the effect of new generation, non-steroidal anti-inflammatory drug (NSAID) meloxicam on the albumin conformation and ligand binding. In the present work the interaction of meloxicam with HSA in aqueous solution at physiological pH has been investigated through circular dichroism and fluorescence spectroscopy. The strong quenching of the fluorescence clearly indicated that the binding of the drug to HSA changed the microenvironment of tryptophan residue and the tertiary structure of HSA. This was confirmed by the destabilization of the warfarin binding site. CD and fluorescence spectroscopic results showed marked reductions (about 40% decrease in the CD Cotton effect intensity, and approximately 15% decrease of the fluorescence intensity) in the affinity of albumin for bilirubin upon meloxicam binding. The strong inhibition of warfarin and ANS bound to protein after meloxicam modification compared with aspirin confirms that the binding site of both drugs is not the same.
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http://dx.doi.org/10.1016/j.jpba.2009.12.025DOI Listing
June 2010
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