Publications by authors named "Katarzyna Taranta-Janusz"

43 Publications

Tumor Necrosis Factor-Like Weak Inducer of Apoptosis and Selected Cytokines-Potential Biomarkers in Children with Solitary Functioning Kidney.

J Clin Med 2021 Feb 1;10(3). Epub 2021 Feb 1.

Department of Paediatrics and Nephrology, Medical University of Bialystok, Kilinskiego 1 st., 15-089 Białystok, Poland.

This study was performed to explore serum tumor necrosis factor-like weak inducer of apoptosis (TWEAK) and its dependent cytokines urinary excretion: monocyte chemoattractant protein- (MCP-1) and regulated on activation, normal T cell expressed and secreted chemokine (RANTES) with their relation to the kidney function parameters in children with solitary functioning kidney (SFK). The study included 80 children and adolescents (median age 9.75 year) with congenital and acquired (after surgical removal) SFK. Serum TWEAK and urinary MCP-1 and RANTES levels were significantly higher in SFK patients ( < 0.05). The serum TWEAK was positively related to serum creatinine ( = 0.356; < 0.001). Moreover, in SFK the receiver operating characteristic analyses revealed good diagnostic profile for serum TWEAK with AUC (Area Under The Curve)-0.853, uRANTES-0.757, and for RANTES/cr.: AUC-0.816. Analysis carried out to identify children with impaired renal function (albuminuria and/or decreased estimated glomerular filtration rate < 90 mL/min/1.73 m and/or hypertension) showed good profile for TWEAK (AUC-0.79) and quite good profile for uRANTES and RANTES/cr. (AUC 0.66 and 0.631, respectively). This is the first study investigating serum TWEAK and urinary excretion of MCP-1 and RANTES together in children with SFK. Obtained results indicate that TWEAK and RANTES may serve as potential markers of renal impairment.
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http://dx.doi.org/10.3390/jcm10030497DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7866991PMC
February 2021

Urine NGAL and KIM-1-Tubular Injury Biomarkers in Long-Term Survivors of Childhood Solid Tumors: A Cross-Sectional Study.

J Clin Med 2021 Jan 21;10(3). Epub 2021 Jan 21.

Department of Pediatric Oncology and Hematology, Medical University of Bialystok, 15-274 Białystok, Poland.

The deterioration of renal function after childhood solid tumors treatment is the result of using the intensive multimodal therapy. In recent years, urinary kidney injury molecule-1 (KIM-1) and urinary neutrophil gelatinase-associated lipocalin (NGAL) have been introduced as potential promising biomarkers of early kidney damage. The aim of the present study was to determine whether anticancer treatment has any effect on the concentration of KIM-1 and NGAL and its association with renal impairment in survivors of childhood solid tumors. Sixty patients previously treated for solid tumors were involved in this study. The median time after end of treatment was 8.35 years. Urine KIM-1 and NGAL levels were measured using immunoenzymatic ELISA commercial kits. Higher levels of urine NGAL, KIM-1/cr. (creatinine), and NGAL/cr. ratios were found in comparison with healthy controls ( < 0.0001). Among all subjects, 23% were found to have decreased estimated glomerular filtration rate (eGFR). A strong correlation between KIM-1/cr. and a cumulative dose of ifosfamide was observed (r = 0.865, < 0.05). In addition, a moderate correlation between NGAL/cr. and a cumulative dose of cisplatin was identified (r = 0.534, < 0.05). The AUC for KIM-1/cr. was 0.52, whereas NGAL/cr. showed a diagnostic profile describing the AUC of 0.67. Univariable regression showed significant associations between NGAL/cr. ratio and subjects after unilateral nephrectomy (coeff. 63.8, = 0.007), cumulative dose of cisplatin (coeff. 0.111, = 0.033), and age at diagnosis (coeff. 3.75, = 0.023). The multivariable model demonstrated only cumulative dose of cisplatin as an independent factor influence on NGAL/cr. ratio. The results of our study showed increased levels of urine KIM-1 and NGAL many years after completion of the childhood solid tumors treatment, which correlated positively with a cumulative dose of ifosfamide and cisplatin. This study also suggests that unilateral nephrectomy could affect the concentration of the studied biomarkers.
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http://dx.doi.org/10.3390/jcm10030399DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7866176PMC
January 2021

Mild X-linked Alport syndrome due to the COL4A5 G624D variant originating in the Middle Ages is predominant in Central/East Europe and causes kidney failure in midlife.

Kidney Int 2020 Dec 10. Epub 2020 Dec 10.

Rare Diseases Centre, Medical University of Gdańsk, Gdańsk, Poland; Clinical Genetics Unit, Department of Biology and Medical Genetics, Faculty of Medicine, Medical University of Gdańsk, Gdańsk, Poland. Electronic address:

A study of 269 children enrolled into a National Registry for children with persistent glomerular hematuria identified 131 individuals with genetically confirmed X-linked Alport Syndrome. A single variant c.1871G>A p.Gly624Asp (G624D) in COL4A5 was predominant and accounted for 39% of X-linked Alport Syndrome in unrelated Polish families (44 of 113). To evaluate its origins, the genetic variation in a 2.79 Mb segment encompassing the COL4A5 locus on chromosome X was assessed. All G624D alleles were found on the same rare haplotype background, indicating a founder effect dating back to the 12-13th century. The phenotypic data of 131 children with X-linked Alport Syndrome and their 195 affected adult relatives revealed that the G624D variant was associated with a significantly milder clinical course in comparison to other pathogenic COL4A5 variants. Furthermore the clinical course of this genetically uniform cohort was milder than that observed in individuals with other COL4A5 missense mutations. In spite of the benign clinical manifestation throughout childhood and early adulthood, the G624D variant confers significant risk for both kidney failure and deafness in males, albeit 20-30 years later than that observed in individuals with other COL4A5 pathogenic variants (50% cumulative risk of starting dialysis at 54 years (95% confidence interval: 50-62) v. 26 years (95% confidence interval: 22-30)). Thus, males with G624D are candidates for existing and emerging therapies for Alport Syndrome.
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http://dx.doi.org/10.1016/j.kint.2020.10.040DOI Listing
December 2020

Urine NGAL and KIM-1: tubular injury markers in acute lymphoblastic leukemia survivors.

Cancer Chemother Pharmacol 2020 Dec 14;86(6):741-749. Epub 2020 Oct 14.

Department of Pediatric Oncology and Hematology, Medical University of Bialystok, ul. Waszyngtona 17, 15-274, Białystok, Poland.

Introduction: Nephrotoxicity is a potential adverse effect of anticancer treatment in childhood. Cytostatics, abdominal radiotherapy, total body irradiation (TBI) and some agents used in supportive care may induce acute kidney injury (AKI) or lead to chronic kidney disease (CKD). The aim of this study was to test the hypothesis whether urinary kidney injury molecule-1 (KIM-1) and neutrophil gelatinase-associated lipocalin (NGAL) are increased in acute lymphoblastic leukemia (ALL) survivors.

Method: The study cohort consisted of 86 patients (42 females) previously treated for ALL. The median time after cessation of treatment was 6.55 (IQR: 1.96-9.93) years and median age at the time of study: 12 (IQR: 6.76-16.00). The control group included 53 healthy peers. Immunoenzymatic ELISA commercial kits were used to measure urine KIM-1 and NGAL levels.

Results: The median levels of urine uNGAL (p < 0.05), uNGAL/creatinine (cr.) ratio (p < 0.0001) and uKIM-1/creatinine ratio (p < 0.0001) were significantly higher in ALL survivors in comparison with healthy controls. Female patients had significantly higher levels of NGAL and NGAL/cr. than males (mean 8.42 ± 7.1 vs. 4.59 ± 4.5 ng/mL and 86.57 ± 77 vs. 37.7 ± 37 ng/mg, respectively; p < 0.01). Of all the study participants, 11 (13%) presented eGFR below 90 mL/min/1.73 m. The NGAL/cr. ratio seemed to be the best predictor of decreased eGFR (AUC = 0.65). The cumulative dose of methotrexate and cyclophosphamide did not predict the values of the urine NGAL, NGAL/cr., KIM-1/cr. and eGFR. Five years after the end of treatment, the patients had higher levels of uKIM-1 (1.02 ± 0.8 vs. 0.62 ± 0.6 ng/mL, p < 0.01), uNGAL (7.9 ± 6.7 vs. 4.6 ± 5 ng/mL, p < 0.01) and lower eGFR (114 ± 29 vs. 134 ± 35 mL/min/1.73 m, p < 0.01) in comparison with ALL survivors with the observation period of less than 5 years.

Conclusion: We demonstrated that ALL survivors have higher levels of urine NGAL, NGAL/cr. and uKIM-1/cr. ratio as compared to the control group. Further long-term follow-up studies are necessary to assess the significance of the NGAL and KIM-1 and their relationship to kidney damage after anticancer treatment in childhood.
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http://dx.doi.org/10.1007/s00280-020-04164-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7603460PMC
December 2020

Severe neurological outcomes after very early bilateral nephrectomies in patients with autosomal recessive polycystic kidney disease (ARPKD).

Sci Rep 2020 09 29;10(1):16025. Epub 2020 Sep 29.

Department of Pediatrics, Faculty of Medicine, University Hospital Cologne and University of Cologne, Kerpener Str. 62, 50937, Cologne, Germany.

To test the association between bilateral nephrectomies in patients with autosomal recessive polycystic kidney disease (ARPKD) and long-term clinical outcome and to identify risk factors for severe outcomes, a dataset comprising 504 patients from the international registry study ARegPKD was analyzed for characteristics and complications of patients with very early (≤ 3 months; VEBNE) and early (4-15 months; EBNE) bilateral nephrectomies. Patients with very early dialysis (VED, onset ≤ 3 months) without bilateral nephrectomies and patients with total kidney volumes (TKV) comparable to VEBNE infants served as additional control groups. We identified 19 children with VEBNE, 9 with EBNE, 12 with VED and 11 in the TKV control group. VEBNE patients suffered more frequently from severe neurological complications in comparison to all control patients. Very early bilateral nephrectomies and documentation of severe hypotensive episodes were independent risk factors for severe neurological complications. Bilateral nephrectomies within the first 3 months of life are associated with a risk of severe neurological complications later in life. Our data support a very cautious indication of very early bilateral nephrectomies in ARPKD, especially in patients with residual kidney function, and emphasize the importance of avoiding severe hypotensive episodes in this at-risk cohort.
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http://dx.doi.org/10.1038/s41598-020-71956-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7525474PMC
September 2020

Salivary Gland Dysfunction, Protein Glycooxidation and Nitrosative Stress in Children with Chronic Kidney Disease.

J Clin Med 2020 Apr 29;9(5). Epub 2020 Apr 29.

Experimental Dentistry Laboratory, Medical University of Bialystok, 24a M. Sklodowskiej-Curie Street, 15-274 Bialystok, Poland.

This study is the first to evaluate protein glycooxidation products, lipid oxidative damage and nitrosative stress in non-stimulated (NWS) and stimulated whole saliva (SWS) of children with chronic kidney disease (CKD) divided into two subgroups: normal salivary secretion ( = 18) and hyposalivation (NWS flow < 0.2 mL min; = 12). Hyposalivation was observed in all patients with severe renal failure (4-5 stage CKD), while saliva secretion > 0.2 mL/min in children with mild-moderate CKD (1-3 stage) and controls. Salivary amylase activity and total protein content were significantly lower in CKD children with hyposalivation compared to CKD patients with normal saliva secretion and control group. The fluorescence of protein glycooxidation products (kynurenine, N-formylkynurenine, advanced glycation end products), the content of oxidative damage to lipids (4-hydroxynonneal, 8-isoprostanes) and nitrosative stress (peroxynitrite, nitrotyrosine) were significantly higher in NWS, SWS, and plasma of CKD children with hyposalivation compared to patients with normal salivary secretion and healthy controls. In CKD group, salivary oxidation products correlated negatively with salivary flow rate, -amylase activity and total protein content; however, salivary oxidation products do not reflect their plasma level. In conclusion, children with CKD suffer from salivary gland dysfunction. Oxidation of salivary proteins and lipids increases with CKD progression and deterioration of salivary gland function.
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http://dx.doi.org/10.3390/jcm9051285DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7287576PMC
April 2020

A Case-Control Study of Salivary Redox Homeostasis in Hypertensive Children. Can Salivary Uric Acid be a Marker of Hypertension?

J Clin Med 2020 Mar 19;9(3). Epub 2020 Mar 19.

Experimental Dentistry Laboratory, Medical University of Bialystok, 24a M. Sklodowskiej-Curie Street, 15-274 Bialystok, Poland.

Oxidative stress plays a critical role in the pathogenesis of hypertension; however, there are no data on salivary redox homeostasis and salivary gland function in children with hypertension. A total of 53 children with hypertension and age- and sex-matched controls were classified for the study. The antioxidant barrier and oxidative/nitrosative stress were evaluated in non-stimulated (NWS) and stimulated (SWS) whole saliva, plasma, and erythrocytes, with Student's t-test and Mann-Whitney U-test used for statistical analysis. We demonstrated that the activities of superoxide dismutase, catalase, and peroxidase were significantly higher in NWS, SWS, and erythrocytes of children with hypertension, similar to oxidative damage in proteins (advanced glycation end products) and lipids (malondialdehyde) as well as nitrosative stress markers (peroxynitrite and nitrotyrosine). The level of uric acid (UA) was significantly higher in NWS, SWS, and plasma of children with hypertension. UA concentration in SWS correlated positively with systolic and diastolic blood pressure and UA content in plasma. This parameter differentiates children with hypertension from healthy controls (AUC = 0.98) with a high degree of sensitivity (94%) and specificity (94%). Stimulated salivary flow was significantly lower in the hypertension group, similar to total protein content and salivary amylase activity. In summary, childhood hypertension is associated with hyposalivation as well as disturbances in antioxidant defense and enhanced oxidative/nitrosative damage both in the plasma/erythrocytes as well as saliva. Salivary UA may be a potential biomarker of hypertension in children.
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http://dx.doi.org/10.3390/jcm9030837DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7141280PMC
March 2020

Dysfunction of Salivary Glands, Disturbances in Salivary Antioxidants and Increased Oxidative Damage in Saliva of Overweight and Obese Adolescents.

J Clin Med 2020 Feb 17;9(2). Epub 2020 Feb 17.

Department of Hygiene, Epidemiology and Ergonomics, Medical University of Bialystok, 15-222 Bialystok, Poland.

Obesity is inseparably connected with oxidative stress. This process may disturb the functioning of the oral cavity, although the effect of oxidative stress on salivary gland function and changes in the qualitative composition of saliva are still unknown. Our study is the first to evaluate salivary redox homeostasis in 40 overweight and obese adolescents and in the age- and gender-matched control group. We demonstrated strengthening of the antioxidant barrier (superoxide dismutase, catalase, peroxidase, uric acid, total antioxidant capacity (TAC)) with a simultaneous decrease in reduced glutathione concentration in saliva (non-stimulated/stimulated) in overweight and obese teenagers compared to the controls. The concentration of the products of oxidative damage to proteins (advanced glycation end products), lipids (malondialdehyde, 4-hydroxynonenal) and DNA (8-hydroxydeoxyguanosine) as well as total oxidative status were significantly higher in both non-stimulated and stimulated saliva as well as plasma of overweight and obese adolescents. Importantly, we observed more severe salivary and plasma redox alterations in obese adolescents compared to overweight individuals. In the study group, we also noted a drop in stimulated salivary secretion and a decrease in total protein content. Interestingly, dysfunction of parotid glands in overweight and obese teenagers intensified with the increase of BMI. We also showed that the measurement of salivary catalase and TAC could be used to assess the central antioxidant status of overweight and obese adolescents.
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http://dx.doi.org/10.3390/jcm9020548DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7074099PMC
February 2020

Salivary FRAP as A Marker of Chronic Kidney Disease Progression in Children.

Antioxidants (Basel) 2019 Sep 18;8(9). Epub 2019 Sep 18.

Department of Conservative Dentistry, Medical University of Bialystok, 15-274 Bialystok, Poland.

Chronic kidney disease (CKD) is one of the most common modern-age diseases in children. Kidney failure does not reveal any symptoms for a long time; therefore, new biomarkers are sought, preferably those reflecting an early stage of CKD. The aim of our study was to evaluate total antioxidant potential as a biomarker differentiating the degree of CKD advancement. The study included 30 children with CKD and a control group matched by age and gender. Non-stimulated saliva (NWS), stimulated saliva (SWS), plasma and urine were used as study material. Total antioxidant potential was determined spectrophotometrically using the FRAP method (ferric ion reducing antioxidant parameter) by measuring total FRAP and uric acid (UA)-independent FRAP (FRAP-UA). We demonstrated that total FRAP, FRAP-UA and UA were significantly higher in stimulated saliva, as well as urine of CKD patients compared to the controls. These biomarkers increase with the progression of chronic kidney disease and their concentration in SWS reflects their content in urine. Interestingly, salivary FRAP and uric acid clearly differentiate between various stages of CKD as well as between healthy and ill children. Special attention should be paid to total FRAP which-measured in SWS-distinguishes patients with mildly to moderately decreased kidney function from those with severe renal impairment (AUC = 1, sensitivity = 100%, specificity = 100%). Although salivary FRAP may be a potential CKD biomarker in children, further studies are needed in a larger group of patients.
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http://dx.doi.org/10.3390/antiox8090409DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6769502PMC
September 2019

Rare Variants in BNC2 Are Implicated in Autosomal-Dominant Congenital Lower Urinary-Tract Obstruction.

Am J Hum Genet 2019 05;104(5):994-1006

Department of Pediatrics, Children's Hospital, University Hospital Bonn, 53113 Bonn, Germany; Institute of Human Genetics, University of Bonn, 53127 Bonn, Germany. Electronic address:

Congenital lower urinary-tract obstruction (LUTO) is caused by anatomical blockage of the bladder outflow tract or by functional impairment of urinary voiding. About three out of 10,000 pregnancies are affected. Although several monogenic causes of functional obstruction have been defined, it is unknown whether congenital LUTO caused by anatomical blockage has a monogenic cause. Exome sequencing in a family with four affected individuals with anatomical blockage of the urethra identified a rare nonsense variant (c.2557C>T [p.Arg853]) in BNC2, encoding basonuclin 2, tracking with LUTO over three generations. Re-sequencing BNC2 in 697 individuals with LUTO revealed three further independent missense variants in three unrelated families. In human and mouse embryogenesis, basonuclin 2 was detected in lower urinary-tract rudiments. In zebrafish embryos, bnc2 was expressed in the pronephric duct and cloaca, analogs of the mammalian lower urinary tract. Experimental knockdown of Bnc2 in zebrafish caused pronephric-outlet obstruction and cloacal dilatation, phenocopying human congenital LUTO. Collectively, these results support the conclusion that variants in BNC2 are strongly implicated in LUTO etiology as a result of anatomical blockage.
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http://dx.doi.org/10.1016/j.ajhg.2019.03.023DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6506863PMC
May 2019

Urinary procollagen III aminoterminal propeptide and β-catenin - New diagnostic biomarkers in solitary functioning kidney?

Adv Med Sci 2019 Mar 6;64(1):189-194. Epub 2019 Feb 6.

Department of Pediatrics and Nephrology, Medical University of Bialystok, Poland.

Purpose: We aimed at evaluating urinary levels of procollagen III aminoterminal propeptide (PIIINP) and β-catenin and the relationship between these markers and clinical and laboratory variables in children with a solitary functioning kidney (SFK).

Patients And Methods: The study group consisted of 98 (M/F: 62/36) children with an SFK with a median age of 8 years. An age-matched control group contained 54 healthy peers. Urinary levels of procollagen III aminoterminal propeptide and β-catenin were measured using a commercially available immunoassay kit.

Results: The urinary values of PIIINP (UPIIINP) were significantly increased in patients with SFK versus controls (p < 0.01). Our analysis revealed no significant differences in urinary β-catenin levels between the SFK patients and control subjects (p > 0.05). Only urinary PIIINP levels were correlated to renal function tests, such as serum creatinine, urea, uric acid, and estimated glomerular filtration rate (p<0.05).

Conclusions: An increased urinary level of PIIINP may indicate early kidney impairment in children with SFK. Urinary β-catenin does not seem to play any important role as a marker of renal function in children with SFK. Further long-term studies are required in order to evaluate the clinical usefulness of these markers and their predictive value of chronic kidney disease (CKD) progression.
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http://dx.doi.org/10.1016/j.advms.2018.10.002DOI Listing
March 2019

Activity of lysosomal exoglycosidases in the urine of healthy normotensive and pre-hypertensive children.

Adv Med Sci 2019 Mar 9;64(1):24-31. Epub 2018 Oct 9.

Department of Pediatrics and Nephrology, Medical University of Bialystok, Bialystok, Poland.

Purpose: We investigated the relationship between blood pressure (BP) and the activity of lysosomal exoglycosidases: N-acetyl-β-hexosaminidase (HEX), its isoenzymes A (HEX A) and B (HEX B), α-fucosidase (FUC), β-galactosidase (GAL), β-glucuronidase (GLU) and α-mannosidase (MAN) in pre-hypertensive (high normal blood pressure - HNBP) and normal blood pressure (NBP) children.

Material And Methods: The study was carried out with urine samples collected from 176 children, aged 6-17.9 years, divided into 2 groups: 42 HNBP and 134 NBP subjects. The children were stratified depending on systolic and diastolic BP (SBP; DBP): HNBP (SBP and/or DBP greater than or equal to the 90th percentile, but less than the 95th percentile) for sex, age, and height; and NBP (SBP and DBP less than the 90th centile). The activities of lysosomal exoglycosidases were determined by the colorimetric method, and expressed in pKat/mL and pKat/μgCr.

Results: The activity of urinary HEX A in HNBP group was significantly higher than in NBP (p < 0.05). The HNBP group showed significant positive correlation between HEX, HEX A (pKat/mL) and SBP. AUC for HEX A was 0.616, cut-off value -29.351 pKat/mL (sensitivity 51.2%, specificity 71.8%), and 0.589, cut-off value -0.054 pKat/μgCr (sensitivity 31.7%, specificity 86.3%).

Conclusions: This is the first report of the relationship between BP and the activity of urinary lysosomal exoglycosidases: HEX, HEX A and HEX B, FUC, GAL, GLU, and MAN in healthy children and adolescents. It seems that HEX A (pKat/mL) can be used as a useful tool in identifying children with HNBP.
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http://dx.doi.org/10.1016/j.advms.2018.09.001DOI Listing
March 2019

Salivary Biomarkers of Oxidative Stress in Children with Chronic Kidney Disease.

J Clin Med 2018 Aug 10;7(8). Epub 2018 Aug 10.

Department of Conservative Dentistry, Medical University of Bialystok, 24a M. Sklodowskiej-Curie Street, 15-274 Bialystok, Poland.

There are still missing non-invasive biomarkers of chronic kidney disease (CKD) in children. Therefore, the aim of the study was to evaluate oxidative stress indicators in the non-stimulated (NWS) and stimulated saliva (SWS) of CKD children ( = 25) and healthy controls ( = 25). Salivary antioxidants (catalase (CAT), peroxidase (Px), superoxide dismutase (SOD), uric acid (UA), reduced glutathione (GSH), albumin), redox status (total antioxidant capacity (TAC), total oxidant status (TOS), oxidative stress index (OSI)), and oxidative damage products (advanced glycation end products (AGE), advanced oxidation protein products (AOPP), malondialdehyde (MDA)) were evaluated. We have demonstrated the significantly higher activity of SWS GPx and SOD, as well as elevated concentrations of UA and albumin in NWS and SWS of CKD children vs. the control group. TAC, TOS and OSI were significantly higher only in SWS, while oxidative damage products (AGE, AOPP and MDA) were significantly higher in both NWS and SWS of CKD children. ROC analysis showed a considerably high diagnostic value of AOPP in both NWS and SWS of CKD children compared to controls (AUC = 0.92; 0.98). CKD is responsible for disturbances in salivary antioxidant systems and oxidative damage to proteins and lipids. Salivary AOPP can be a potential biomarker of CKD in children.
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http://dx.doi.org/10.3390/jcm7080209DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6111793PMC
August 2018

Risk Factors for Early Dialysis Dependency in Autosomal Recessive Polycystic Kidney Disease.

J Pediatr 2018 08 9;199:22-28.e6. Epub 2018 May 9.

Department of Pediatrics, University Hospital of Cologne, Cologne, Germany; Center for Molecular Medicine, University Hospital of Cologne, Cologne, Germany.

Objective: To identify prenatal, perinatal, and postnatal risk factors for dialysis within the first year of life in children with autosomal recessive polycystic kidney disease (ARPKD) as a basis for parental counseling after prenatal and perinatal diagnosis.

Study Design: A dataset comprising 385 patients from the ARegPKD international registry study was analyzed for potential risk markers for dialysis during the first year of life.

Results: Thirty-six out of 385 children (9.4%) commenced dialysis in the first year of life. According to multivariable Cox regression analysis, the presence of oligohydramnios or anhydramnios, prenatal kidney enlargement, a low Apgar score, and the need for postnatal breathing support were independently associated with an increased hazard ratio for requiring dialysis within the first year of life. The increased risk associated with Apgar score and perinatal assisted breathing was time-dependent and vanished after 5 and 8 months of life, respectively. The predicted probabilities for early dialysis varied from 1.5% (95% CI, 0.5%-4.1%) for patients with ARPKD with no prenatal sonographic abnormalities to 32.3% (95% CI, 22.2%-44.5%) in cases of documented oligohydramnios or anhydramnios, renal cysts, and enlarged kidneys.

Conclusions: This study, which identified risk factors associated with onset of dialysis in ARPKD in the first year of life, may be helpful in prenatal parental counseling in cases of suspected ARPKD.
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http://dx.doi.org/10.1016/j.jpeds.2018.03.052DOI Listing
August 2018

Serum Renalase Levels in Adolescents with Primary Hypertension.

Pediatr Cardiol 2018 Aug 10;39(6):1258-1264. Epub 2018 May 10.

Department of Pediatrics and Nephrology, Medical University of Bialystok, 17 Waszyngton Street, 15-274, Białystok, Poland.

The prevalence of hypertension in pediatric populations continues to rise. Recent studies suggest that renalase plays an important role in blood pressure regulation. The aim of this study was to evaluate serum renalase concentrations in hypertensive children. This study was a prospective cohort analysis of 88 adolescents (40 girls; 48 boys) aged 11-18 years, divided into two groups: HT-38 subjects with primary hypertension; and R (reference group)-50 subjects with normal blood pressure. Serum renalase concentration was measured using a commercial enzyme-linked immunosorbent assay kit. Hypertensive patients had higher serum renalase levels (median 29.8 µg/mL; Q1-Q3: 26.1-35.8) than the reference group (median 26.8; Q1-Q3: 22.96-29.4, p < 0.01). Serum renalase was strongly related to serum uric acid levels. In hypertensive patients, serum renalase was positively correlated with 24-h systolic blood pressure (SBP) and 24-h diastolic blood pressure (DBP) and with 24-h SBP and 24-h DBP Z-score (LMS). Our results allow us to conclude that serum renalase correlates with blood pressure elevation. Special attention should be drawn to the correlation between renalase and serum uric acid levels not only in hypertensive, but also in normotensive teenagers. Further studies are needed to answer the question of whether increased serum renalase may be a predisposing factor to hypertension in normotensive patients with hyperuricemia.
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http://dx.doi.org/10.1007/s00246-018-1891-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6096843PMC
August 2018

Urinary exoglycosidases, reference values in healthy children.

Adv Med Sci 2018 Sep 5;63(2):224-229. Epub 2018 Feb 5.

Department of Pediatrics and Nephrology, Medical University of Bialystok, Bialystok, Poland.

Purpose: The purpose of the study was to determine the effect of age on lysosomal exoglycosidase activities: α-fucosidase, β-galactosidase, β-glucuronidase and α-mannosidase in healthy children and adolescents.

Material And Methods: Urine samples were collected from 203 healthy children and adolescents (girls = 99, boys = 104), aged six months to 17.9 years. The activities of α-fucosidase, β-galactosidase, β-glucuronidase and α-mannosidase were determined by colorimetric method and expressed in pKat/μg of creatine (pKat/μg Cr.).

Results: Urinary α-fucosidase, β-galactosidase, β-glucuronidase and α-mannosidase activities (pKat/μg Cr.) were the highest in children below 3 years of age in comparison to the remaining age groups. There was a statistically significant negative correlation between urinary α-fucosidase, β-galactosidase, β-glucuronidase and α-mannosidase (pKat/μg Cr.) and age (r = -0.36; r = -0.36; r = -0.35; r = -0.35; at p < 0.0001, respectively). In addition, we constructed the reference values for urinary activity of α-fucosidase, β-galactosidase, β-glucuronidase and α-mannosidase (pKat/μg Cr.) in percentiles according to age in 3-year intervals.

Conclusions: Our study is the first to show reference values for urinary α-fucosidase, β-galactosidase, β-glucuronidase and α-mannosidase in children and adolescents.
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http://dx.doi.org/10.1016/j.advms.2018.01.004DOI Listing
September 2018

Pediatric reference data on activity of urinary N-acetyl-β-D-hexosaminidase and its isoenzymes.

Adv Med Sci 2018 Mar 29;63(1):94-99. Epub 2017 Aug 29.

Department of Pediatrics and Nephrology, Medical University of Bialystok, Bialystok, Poland.

Purpose: The objective of the study was to establish age - dependent values of the urinary lysosomal exoglycosidases activities: N-acetyl-β-D-hexosaminidase (HEX) and its isoenzyme A (HEX A) as well as isoenzyme B (HEX B) in healthy children and adolescents.

Material And Methods: The study was performed using a random sample of 203 healthy children and adolescents (girls=99, boys=104), aged six months to 17.9 years. The activities of HEX, HEX A and HEX B were determined by a colorimetric method. The activities of the urinary HEX and its isoenzymes were expressed in pKat/μg of creatinine (pKat/μg Cr).

Results: Median concentrations of urinary HEX, and its HEX A, HEX B isoenzymes in particular age groups were analyzed using ANOVA. Urinary HEX, HEX A and HEX B activities (pKat/μg Cr) were the highest in children below 3 years, in comparison to remaining age groups. There were statistically significant negative correlations between urinary HEX, HEX A as well as HEX B and age (r=-0.24, p<0.001 (HEX); r=-0.20, p<0.01 (HEX A); r=-0.26, p<0.001 (HEX B), respectively. We constructed the reference values for urinary activity of HEX, HEX A and HEX B (pKat/μg Cr) in centiles according to age, in three-year intervals.

Conclusions: Reported data present, for the first time, reference values for urinary activities of HEX and its isoenzymes HEX A and HEX B in children and adolescent.
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http://dx.doi.org/10.1016/j.advms.2017.06.007DOI Listing
March 2018

Determining normal values of urinary phosphorus excretion in 3913 healthy children aged 2-18 to aid early diagnosis and treatment for urolithiasis.

Acta Paediatr 2017 Jul 2;106(7):1170-1175. Epub 2017 May 2.

Department of Paediatrics and Nephrology, Medical University of Bialystok, Bialystok, Poland.

Aim: This study determined the specific reference values for urinary phosphorus excretion in healthy children and adolescents aged 2-18 years and evaluated whether they changed with age during growth and were gender dependent.

Methods: We enrolled 3913 healthy children and adolescents aged 2-18 years to this study. The study population was divided into age groups, and the analysis was performed in one-year periods, separately for boys and girls. Urinary phosphorus excretion was analysed using four categories: P1 in mmol/24 hour units, P2 in mmol/kg/24 hours, P3 in mmol/1.73 m /24 hours and P4 in mmol/mmol creatinine.

Results: Clear differences in urinary exertion for girls and boys were observed as well as systematic changes with age. The boys presented with significantly higher daily urinary phosphorus excretion independent of its manner of expression (p < 0.001). The median urinary phosphorus (P1) rose with age (p < 0.001). Percentile tables of phosphorous exertion are presented.

Conclusion: This was the largest study of urinary phosphate excretion based on a randomly selected sample of girls and boys aged 2-18 years. It highlights the importance of determining phosphorus reference values for children of different ages to provide early diagnosis and treatment for urolithiasis.
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http://dx.doi.org/10.1111/apa.13856DOI Listing
July 2017

Is urine intercellular adhesion molecule-1 a marker of renal disorder in children with ureteropelvic junction obstruction?

Biomarkers 2016 3;21(2):123-8. Epub 2015 Dec 3.

a Department of Pediatrics and Nephrology , Medical University of Białystok , Białystok , Poland.

Aim: We aimed to investigate whether urine intercellular adhesion molecule-1 (ICAM-1) might serve as a marker of renal disorder in children with ureteropelvic junction obstruction.

Material And Methods: Twenty-nine children with severe hydronephrosis (HN) were compared with 23 participants with mild HN and with 19 healthy peers.

Results: Urine ICAM-1/uCre levels were significantly higher in HN children than healthy controls (P<0.01), and in severe HN when compared with mild HN (p<0.05).

Conclusions: It seemed to us that uICAM-1 is a biomarker of renal disorder, and might have the potential to predict which patients will require surgery.
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http://dx.doi.org/10.3109/1354750X.2015.1118543DOI Listing
December 2016

Urine exoglycosidases are potential markers of renal tubular injury in children with ureteropelvic junction obstruction.

Acta Paediatr 2015 Nov 29;104(11):e518-23. Epub 2015 Jul 29.

Department of Pediatrics and Nephrology, Medical University of Białystok, Białystok, Poland.

Aim: Hydronephrosis caused by ureteropelvic junction obstruction (UPJO) is an important problem in children and young adults. The aim of this pilot study was to determine the urine profiles of a number of lysosomal exoglycosidases to see whether they indicated tubular renal damage in children with UPJO.

Methods: We measured lysosomal exoglycosidases urine activities in 32 patients with UPJO, dividing them into three groups. The surgical group comprised 16 children with severe hydronephrosis who required surgery, the nonsurgical group comprised 16 patients with mild hydronephrosis, and the reference group comprised 42 healthy children. The following indicators were measured: N-acetyl-β-hexosaminidase and its A and B isoenzymes, α-fucosidase, β-galactosidase, α-mannosidase and β-glucuronidase.

Results: The urine activities of all exoglycosidases were significantly higher in children with UPJO than children in the reference group (p < 0.01). A strong positive correlation was also found between most of the urine exoglycosidases and the urine albumin/creatinine ratio (p < 0.01).

Conclusion: Our findings demonstrated that children with UPJO showed increased renal activities of assessed exoglycosidases, which correlated positively with the urine albumin/creatinine ratio. A larger multicentre study is required to confirm the clinical applications of these observations.
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http://dx.doi.org/10.1111/apa.13068DOI Listing
November 2015

The effects of breastfeeding on serum asymmetric dimethylarginine levels and body composition in children.

Breastfeed Med 2015 Jan-Feb;10(1):38-44. Epub 2014 Oct 30.

Department of Pediatrics and Nephrology, Medical University of Białystok , Białystok, Poland .

Introduction: The purpose of this work was to investigate the association of serum asymmetric dimethylarginine (ADMA) and high-sensitivity C-reactive protein (hs-CRP) levels with duration of breastfeeding and body composition in children.

Patients And Methods: The study group consisted of 88 patients with a median age of 12 months (42 boys, 46 girls), classified as never breastfed or fully breastfed. ADMA and hs-CRP were measured by immunoenzymatic enzyme-linked immunosorbent assay commercial kits. Body composition analysis was performed by bioelectrical impedance.

Results: We found significantly higher serum ADMA levels but not serum hs-CRP levels in never breastfed compared with the fully breastfed group (p<0.05). Serum ADMA was inversely associated with high-density lipoprotein-cholesterol levels and breastfeeding duration (p<0.05). Positive correlation was found between ADMA and body fat mass (p<0.05).

Conclusions: In never breastfed children, increased ADMA is observed; however, further studies are needed to assess whether breastfeeding duration affects body fat and body composition at older ages.
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http://dx.doi.org/10.1089/bfm.2014.0128DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4307157PMC
June 2016

Increased circulating inflammatory markers may indicate that formula-fed children are at risk of atherosclerosis.

Acta Paediatr 2014 Aug 26;103(8):e354-8. Epub 2014 May 26.

Department of Paediatrics and Nephrology, Medical University of Bialystok, Bialystok, Poland.

Aim: The aim of this study was to determine whether formula-fed children have higher serum monocyte chemoattractant protein-1 (MCP-1) and uric acid levels than breast-fed children and to evaluate the association between these inflammatory markers and breastfeeding duration.

Methods: The study group consisted of 87 patients aged between five and 32 months. Participants were divided into breast-fed and formula-fed groups and into age groups of ≤12 months and >12 months. MCP-1 was measured by the commercial immunoenzymatic ELISA kit, and uric acid was assessed using the colorimetric method.

Results: Children in the formula-fed group had statistically significant higher serum MCP-1 and uric acid levels than breast-fed children (p < 0.01 and p < 0.05, respectively). Anthropometric parameters were comparable in both groups. Serum MCP-1 and uric acid levels were negatively correlated with duration of breastfeeding (p < 0.01 and p < 0.05). There was a positive relationship between serum MCP-1 and uric acid concentrations (r = 0.27, p < 0.05).

Conclusion: Increased circulating inflammatory markers may indicate that formula-fed children are at risk of atherosclerosis. However, further studies are needed.
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http://dx.doi.org/10.1111/apa.12680DOI Listing
August 2014

Is symmetric dimethylarginine a sensitive biomarker of subclinical kidney injury in children born with low birth weight?

Biomarkers 2014 May 28;19(3):231-5. Epub 2014 Mar 28.

Department of Pediatric Nephrology, Medical University of Bialystok , Bialystok , Poland.

Objective: The aim of this study was to investigate if Symmetric Dimethylarginine (SDMA) is a sensitive biomarker of renal function and may predict subclinical kidney injury in low birth weight (LBW) children.

Methods: We studied 68 LBW children and 20 children as reference group. An enzyme-linked immunosorbent assay was used to measure serum SDMA and Cystatin C (Cys C).

Results: SDMA levels were higher in study groups compared to reference groups. There was a strong correlation between SDMA and Cys C, also SDMA negatively correlated with eGFR.

Conclusion: Elevated SDMA concentration may play an important role in pathogenesis of chronic kidney disease.
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http://dx.doi.org/10.3109/1354750X.2014.899393DOI Listing
May 2014

New tubular injury markers in children with a solitary functioning kidney.

Pediatr Nephrol 2014 Sep 22;29(9):1599-605. Epub 2014 Mar 22.

Department of Pediatrics and Nephrology, Medical University of Białystok, 15-274, Białystok, Waszyngtona 17, Poland,

Background: The present study aimed to assess whether the urinary profiles of the lysosomal exoglycosidases N‑acetyl‑β‑hexosaminidase (HEX) and its isoenzymes A (HEX A) and B (HEX B), α-fucosidase (FUC), β-galactosidase (GAL), α-mannosidase (MAN), and β- glucuronidase (GLU) are useful biomarkers of tubular dysfunction in children with a solitary functioning kidney (SFK).

Methods: We measured the urinary activity of HEX, its isoenzymes HEX A, HEX B, and FUC, GAL, MAN, and GLU in 52 patients with SFK. Patients were subdivided into two groups: congenital SFK (cSFK)-unilateral renal agenesis and acquired SFK (aSFK)-unilateral nephrectomy. The reference group (RG) contained 60 healthy sex- and age-matched children.

Results: Urinary activity of all exoglycosidases in SFK was significantly higher than in RG (p < 0.05). There were no differences in exoglycosidase activity between cSFK and aSFK (p > 0.05). HEX and its isoenzymes HEX A and HEX B correlated negatively with estimated glomerular filtration rate (eGFR), and all estimated parameters correlated positively with albumin/creatinine ratio (p < 0.001).

Conclusion: Urinary activity of HEX, its isoenzymes HEX A and HEX B, and FUC, GAL, MAN, and GLU is elevated in children with SFK. Long-term follow-up studies in larger groups of children with SFK may help us to better understand their clinical significance.
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http://dx.doi.org/10.1007/s00467-014-2802-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4147209PMC
September 2014

[The role of early-life metabolic programming in the pathogenesis of lifestyle diseases].

Dev Period Med 2014 Oct-Dec;18(4):477-82

Klinika Pediatrii i Nefrologii, Uniwersytecki Dziecięcy Szpital Kliniczny w Białymstoku, ul. Waszyngtona 17, 15-274 Białystok, Poland, tel. (+48 85) 74-50-828, e-mail:

In recent years, a blooming period of genomics brings a window of opportunity to assess predispositions to some diseases in individuals, even before the first symptoms appear. However, a risk of becoming ill is more complex, as the gene expression is modified by epigenetic and environmental factors. Fetal development and first months of life are periods of dynamic growth and significant sensitivity to external factors. According to the theory of early-life metabolic programming, adaptive changes in these stages have lasting health effects. Among many environmental factors, the youngest children's diet plays an important role. Breastfeeding of newborns and infants is an essential part of lifestyle diseases prevention. Constantly increasing number of reports link natural nutrition of the youngest children with less risk of obesity, hypertension, dyslipidemia and insulin resistance in future life. However, further long-term studies taking into account number of bias factors, explaining protective mechanisms of human milk, are needed.
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May 2015

Asymmetric and symmetric dimethylarginine in adolescents with hyperuricemia.

Dis Markers 2013 26;35(5):407-12. Epub 2013 Sep 26.

Department of Pediatrics and Nephrology, Medical University of Bialystok, 17 Waszyngtona Street, 15-274 Bialystok, Poland.

Unlabelled: The purpose of this work was to investigate if in adolescents with hyperuricemia serum levels of asymmetric and symmetric dimethylarginine (ADMA, SDMA) are increased and if their levels correlate with serum uric acid (UA).

Patients And Methods: The study group consisted of 58 hyperuricemic patients aged median 16.15 Q1-Q3 (14-17). The reference group contained 27 healthy individuals with normal serum UA level. ADMA and SDMA were measured by immunoenzymatic ELISA commercial kits and expressed in μ mol/L. Serum UA was measured by the colorimetric method.

Results: In hyperuricemic patients serum ADMA values did not differ between two estimated groups (P > 0.05); however, SDMA was significantly higher than in reference group (P < 0.01). Serum ADMA and SDMA correlated positively with UA (r = 0.34, P < 0.01) (r = 0.31, P < 0.01) and hs-CRP (r = 0.20, P < 0.05) (r = 0.36, P < 0.01), respectively.

Conclusion: We demonstrated increased SDMA but not ADMA levels in adolescents with hyperuricemia and their correlation with serum uric acid levels. However, at the moment it is difficult to answer the question if it is just coexistence of these factors or any mechanism linking uric acid and methylated arginines really exists.
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http://dx.doi.org/10.1155/2013/267697DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3810106PMC
June 2014

Urinary angiotensinogen as a novel marker of obstructive nephropathy in children.

Acta Paediatr 2013 Sep 10;102(9):e429-33. Epub 2013 Jul 10.

Department of Pediatrics and Nephrology, Medical University of Białystok, Białystok, Poland.

Aim: Obstructive nephropathy due to congenital or acquired urinary tract obstruction is one of the most important causes of chronic renal failure in children. There is a need for identification of new noninvasive urinary biomarkers to provide the clinician with fast, specific and reliable diagnostic and prognostic tool. The aim of the study was to determine whether urinary angiotensinogen (uAGT) may be a useful marker of obstruction in children with hydronephrosis (HN) caused by ureteropelvic junction obstruction (UPJO).

Methods: The study cohort consisted of surgical group (SG): 31 children with severe HN who required surgery; nonsurgical group (NSG): 20 patients with mild HN, and reference group (RG): 19 healthy children. Urinary concentrations of angiotensinogen were measured using immunoenzymatic ELISA commercial kit and were expressed in ng/mg Cre (uAGT/uCre).

Results: uAGT/uCre level was higher in SG when compared to NSG (p < 0.01) and healthy participants (SG vs. RG: p < 0.01). The difference between the uAGT/uCre in NSG and RG was not statistically significant (p > 0.05). uAGT/uCre was correlated negatively with differential renal function (r = -0.46; p < 0.01).

Conclusion: The present pilot study has clearly demonstrated that children with UPJO showed increased uAGT levels, which correlated negatively with differential renal function in radionuclide scan.
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http://dx.doi.org/10.1111/apa.12324DOI Listing
September 2013

Urinary cytokine profiles in unilateral congenital hydronephrosis.

Pediatr Nephrol 2012 Nov 29;27(11):2107-2113. Epub 2012 Jun 29.

Department of Pediatrics and Nephrology, Medical University of Białystok, 15-274, Białystok, Waszyngtona 17, Poland.

Background: We aimed to evaluate possible clinical application of urinary monocyte chemotactic protein-1 (MCP-1), osteopontin (OPN), and regulated upon activation, normal T-cell expressed and secreted (RANTES) chemokine as useful noninvasive markers in children with congenital hydronephrosis (HN) caused by ureteropelvic junction obstruction (UPJO).

Methods: The study cohort consisted of surgical cases (study group 1), comprising 15 children with severe HN who required surgery (median age 1.03 years), conservative cases (study group 2), comprising 21 patients with mild, nonobstructive HN, and control group, comprising 19 healthy children. All children had normal renal function. Urinary (u) concentrations of MCP-1, OPN, and RANTES were measured using immunoenzymatic enzyme-linked immunosorbent assay (ELISA) commercial kits and were expressed in nanograms per milligram creatinine. Increased levels of MCP-1 and OPN were found in children with HN in comparison with study group 2 and controls (p < 0.05). UMCP-1/Cr correlated with half-time (T(1/2)) of the elimination phase of tracer excretion of technetium-99m-mercaptoacetyltriglycine ((99m)Tc-MAG-3) (p < 0.05).

Results: Receiver operator characteristic (ROC) analyses revealed good diagnostic profile for uMCP-1 only in identifying children (<40 %) with abnormal differential renal function (DRF) [area under the curve (AUC) 0.862], and in detecting kidney injury in all examined children (AUC 0.704).

Conclusions: Additional studies with larger number of patients are required to confirm a potential application of uMCP-1 as a promising parameter for early identification of kidney obstruction.
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http://dx.doi.org/10.1007/s00467-012-2230-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3461208PMC
November 2012

Osteopontin and symmetric dimethylarginine plasma levels in solitary functioning kidney in children.

Acta Paediatr 2012 Aug 24;101(8):e369-72. Epub 2012 Apr 24.

Department of Pediatrics and Nephrology, Medical University of Białystok, Białystok, Poland.

Aim: The present study aimed to examine whether plasma osteopontin (pOPN) and symmetric dimethylarginine (pSDMA) are useful biomarkers of renal dysfunction in children with solitary functioning kidney (SFK).

Methods: We measured circulating pOPN and pSDMA in 51 patients with SFK and no other urinary defects. Patients were subdivided into two groups: primary SFK (pSFK) - unilateral renal agenesis (URA), and secondary SFK (sSFK) - unilateral nephrectomy. The control group (C) contained 21 healthy children, with mean age 9.92 ± 4.85 years. Immunoenzymatic ELISA commercial kits were used to measure pOPN and pSDMA concentrations.

Results: Plasma osteopontin and pSDMA levels in children with SFK were higher than those in healthy participants (p < 0.05). There was no difference in pOPN and pSDMA concentrations between patients with pSFK and those with sSFK (p > 0.05). Receiver operator characteristic analyses performed to define the diagnostic efficiency of serum creatinine, pOPN and pSDMA in identifying children with C(cr)  < 90 mL/min/1.73 m(2) among all examined children revealed no differences between all three AUCs (p > 0.05).

Conclusion: Increased pOPN and pSDMA levels were observed in children with SFK. Both pOPN and pSDMA correlated with eGFR; however, the sensitivity and specificity of those markers were not better than those of creatinine.
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http://dx.doi.org/10.1111/j.1651-2227.2012.02690.xDOI Listing
August 2012

Markers of systemic inflammation in children with hyperuricemia.

Acta Paediatr 2012 May 23;101(5):497-500. Epub 2012 Feb 23.

Department of Pediatrics and Nephrology, Medical University of Białystok, Białystok, Poland.

Aim: The purpose of the study was to investigate serum concentrations of the monocyte chemoattractant protein-1 (MCP-1) and high-sensitivity CRP (hs-CRP) in children with hyperuricemia and to evaluate its association with obesity.

Patients And Methods: The study involved 52 hyperuricemic patients with mean age of 15.53 ± 1.7 years. Twenty-seven healthy individuals with normal serum uric acid (SUA) level were selected as the control group (C). Serum MCP-1 and hs-CRP were measured by enzyme-linked immunosorbent assay (ELISA) and immunonephelometry, respectively.

Results: Hyperuricemic patients showed increased sMCP-1 (median: 69.58 pg/mL) and hs-CRP (median: 0.53 mg/L) vs. controls (48.39 pg/mL, 0.24 mg/L; respectively) (p < 0.01). The obese children also presented significantly higher levels of sMCP-1 and hs-CRP (median, 81.69 and 1.18 mg/L, respectively) in comparison with nonobese (median, 59.62 and 0.41 mg/L, respectively; p < 0.01). Only hs-CRP correlated positively with body mass index Z-score (r = 0.33, p < 0.05). Receiver operator characteristic analyses checking the sensitivity and specificity of examined markers for hyperuricemia revealed the higher area under the curve (AUC) for sMCP-1; however, the difference between AUC for sMCP-1 and for hs-CRP was not significant (p > 0.05).

Conclusion: Serum MCP-1 and hs-CRP are elevated in hyperuricemic patients, but the role of obesity in inflammation markers needs further investigation.
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http://dx.doi.org/10.1111/j.1651-2227.2011.02582.xDOI Listing
May 2012