Publications by authors named "Katarzyna Malinowska"

56 Publications

Group-based shame, guilt, and regret across cultures.

Eur J Soc Psychol 2021 Dec 20;51(7):1198-1212. Epub 2022 Feb 20.

Atma Jaya Catholic University Indonesia.

To date, there has been no systematic examination of cross-cultural differences in group-based shame, guilt, and regret following wrongdoing. Using a community sample ( = 1358), we examined people's reported experiences of shame, guilt, and regret following transgressions by themselves and by different identity groups (i.e., family, community, country) in Burkina Faso, Costa Rica, Indonesia, Japan, Jordan, the Netherlands, Poland, and the United States. We assessed whether any variation in this regard can be explained by the relative endorsement of individualistic or collectivistic values at the individual level and at the country level. Our findings suggest that people's reported experience of these emotions mostly depends on the transgression level. We also observe some variation across individuals and countries, which can be partially explained by the endorsement of collectivistic and individualistic values. The results highlight the importance of taking into account individual and cultural values when studying group-based emotions, as well as the identity groups involved in the transgression.
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http://dx.doi.org/10.1002/ejsp.2808DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9306671PMC
December 2021

Serum IL-5, POSTN and IL-33 levels in chronic rhinosinusitis with nasal polyposis correlate with clinical severity.

BMC Immunol 2022 06 25;23(1):33. Epub 2022 Jun 25.

Department of Allergology and Respiratory Rehabilitation, Medical University of Lodz, 90-725, Lodz, Poland.

Background: Chronic rhinosinusitis (CRS) is a group of heterogeneous diseases characterized by epithelial inflammation and tissue eosinophilic infiltration. IL-5, POSTN, and IL-33 are important factors that act as chemoattractants for eosinophils, and a tissue-remodeling protein positively correlated with eosinophils in blood and mediators of eosinophilic infiltration. The aim of the study was to determine the expression of IL-5, POSTN and IL-33, at the gene and protein levels, in eosinophilic CRS with nasal polyps (CRSwNP) and without nasal polyps (CRSsNP), and to correlate this expression with clinical severity.

Materials And Methods: The study included 40 CRSwNP patients and 53 CRSsNP patients and 40 control subjects. The expression of IL-5, POSTN and IL-33 mRNA was determined in sinonasal mucosal samples and in nasal polyp tissue by real-time PCR. Protein levels in the serum of CRSwNP patients were measured by ELISA. Computed tomography was evaluated according to Lund-Mackay scores, and visual analog scale scores were assessed.

Results: NP tissue demonstrated significantly higher IL-5 and POSTN mRNA expression than the sinonasal tissue in the CRSsNP and CRSwNP groups. CRS groups demonstrated elevated IL-33 mRNA expression in comparison to controls irrespective of the presence of NP. No correlation was found between IL-5, POSTN and IL-33 mRNA expression and disease severity. CRSwNP group demonstrated significantly higher serum IL-5, POSTN and IL-33 protein levels than controls, and this corresponds to disease severity.

Conclusion: Serum IL-5, POSTN and IL-33 levels may be important markers for classification of eosinophilic CRSwNP patients, along with disease severity.
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http://dx.doi.org/10.1186/s12865-022-00507-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9233770PMC
June 2022

and Studies on (L.) R. Br. Root Extract against Cancer Cells.

Curr Pharm Biotechnol 2022 ;23(11):1383-1395

Department of Computer Science in Economics, University of Lodz, Lodz, Poland.

Background: Leonotis nepetifolia (L.) R. Br. (Lamiaceae) is a shrub traditionally used to alleviate inflammatory conditions.

Objectives: The present study aimed at investigating the biological activity of methanolic nontransformed and transformed Rhizobium rhizogenes root extracts from L. nepetifolia against human melanoma cells.

Methods: Cytotoxicity and genotoxicity properties, the impact on topoisomerase I activity, and proapoptotic activity were evaluated by the MTT test, comet assay, topoisomerase I assay, and fluorescence-activated cell sorting analysis. Moreover, the expressions of p53 were examined by qPCR and Western blot analysis. Docking studies were conducted to assess the potential interactions of the identified phytochemicals with the p53 binding protein Mdm-2, and computational analyses exhibited their antioxidant potential.

Results: Both extracts showed cytotoxic potential against human melanoma cells, but generally the activity was more potent for transformed roots than untransformed (IC 760 μg/mL and 980 μg/mL, respectively). A similar effect was revealed during the evaluation of genotoxic and proapoptotic properties. Moreover, the expression of p53 was also found to be increased after extract treatment. The most dominant identified compounds in both extracts were as follows: (+)- catechin, p-coumaric acid, m-coumaric acid, and (+)-rosmarinic acid. Docking studies and computational analysis showed that (+)-rosmarinic acid possesses the highest binding affinity to the p53 binding protein, Mdm-2, and exhibits the best antioxidant property from the most commonly identified phytochemicals.

Conclusion: Our findings revealed the potential of L. nepetifolia transformed root extract as a source of bioactive compounds with cytotoxic, genotoxic, and proapoptotic activity against human melanoma cells as well as antioxidant properties.
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http://dx.doi.org/10.2174/1389201023666220304095225DOI Listing
August 2022

Bioaccumulation of Macronutrients in Edible Mushrooms in Various Habitat Conditions of NW Poland-Role in the Human Diet.

Int J Environ Res Public Health 2021 08 23;18(16). Epub 2021 Aug 23.

Faculty of Medicine and Dentistry, Major Medicine, Pomeranian Medical University in Szczecin, Rybacka 1 Street, 70-204 Szczecin, Poland.

Recently, the interest in mushroom consumption has been growing, since their taste and low calorific value are appreciated, but also due to their nutritional value. In determining the usefulness of mushrooms in the human diet, it is important to consider the conditions of their occurrence to perform the assessment of bioaccumulation of minerals. The aim of the study was: (a) to determine the content of selected macronutrients (P, K, Ca, Mg, Na) in fruiting bodies of , , and the soils, (b) to determine their bioaccumulation potential taking into account the habitat conditions, and (c) an attempt to estimate their role in covering the requirement for macronutrients of the human organism. The research material was obtained in the NW of Poland: Uznam and Wolin, the Drawa Plain and the Ińsko Lakeland. In the soil, we determined the content of organic matter, pH, salinity and the content of absorbable and general forms of macronutrients. The content of macronutrients in mushrooms was also determined. Chemical analyses were performed using the generally accepted test methods. The study showed that in NW Poland, grew on the acidic soils of Arenosols, and and grew on Podzols. The uptake of K, Mg and Ca by the tested mushrooms was positively, and P and Na negatively correlated with the content of these elements in the soil. The acidity of the soil affected the uptake of K and Mg by mushrooms. There was no effect of the amount of organic matter in the soil noticed on the content of macronutrients (except sodium) in mushrooms. Among the studied macronutrients, none of the mushrooms accumulated Ca, while P and K were generally accumulated in the highest amounts, regardless of the species. Each of the other elements was usually accumulated at a similar level in the fruiting bodies of the species we studied. The exception was , which accumulated higher amounts of Mg compared to and . Mushrooms can enrich the diet with some macronutrients, especially in P and K.
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http://dx.doi.org/10.3390/ijerph18168881DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8395067PMC
August 2021

Expression of Programmed Death Receptor 1 (PD-1) Gene and Its Ligand (PD-L1) in Patients with Laryngeal Cancer.

Biomolecules 2021 07 1;11(7). Epub 2021 Jul 1.

Department of Allergology and Respiratory Rehabilitation, Medical University of Lodz, 90-725 Lodz, Poland.

(1) Background: The interaction of the programmed death receptor (PD-1) with its ligand 1 (PD-L1) allows cancer cells to escape from the control of the immune system. Research evaluating the expression of immune checkpoint genes in the tissues of laryngeal tumors may contribute to the introduction of new effective immunotherapeutic methods in this group of neoplasms. The aim of this study was to evaluate the expression of the gene for the programmed death receptor (PD-1) and its ligand (PD-L1) in laryngeal tumors (T1, T2, T3) in patients without lymph node involvement and distant metastases. (2) Methods: The study included 73 patients: 39 of them were diagnosed with carcinoma planoepiteliale keratodes (study group) and 34 with nasal septal deviation undergoing septoplasty (control group). Biological material for molecular tests (Real time PCR) was collected during surgical procedures. Furthermore, all study participants completed a questionnaire regarding, among others, smoking and body weight. (3) Results: Gene expression for programmed death receptor 1 (PD-1) and its ligand 1 (PD-L1) was, statistically, significantly higher ( < 0.0001) in tumor tissue than in unchanged mucosa. Moreover, it was found that the greater the tumor size, the higher the expression level of the tested molecules. (4) Conclusions: Although further research on the role of the PD-1/PD-L1 pathway in laryngeal tumors is necessary, the presented reports are promising and may constitute a contribution to considerations on the introduction of targeted immunotherapy with anti-PD1 and anti-PD-L1 monoclonal antibodies in the treatment of these tumors.
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http://dx.doi.org/10.3390/biom11070970DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8301886PMC
July 2021

Correlational nutritional relationships and interactions between expansive holoparasite Orobanche laxissima and woody hosts on metal-rich soils.

Phytochemistry 2021 Oct 23;190:112844. Epub 2021 Jul 23.

Center for Research and Conservation of Biodiversity, Department of Enviromental Biology, Institute of Biology, Jan Kochanowski University, Uniwersytecka 7 Street, PL-25-406, Kielce, Poland. Electronic address:

Plant parasitism by other plants, combined with abiotic environmental stress, offers a unique opportunity to study correlational nutritional relationships in terms of parasite-host interactions and their functional roles in nutrient cycling in ecosystems. Our study analysed the transfer of selected mineral elements, including heavy metals, from soil to different organs in hosts (Punica granatum and Fraxinus angustifolia) and from hosts to the expansive holoparasite (Orobanche laxissima) in cinnamonic soil habitats in Georgia (Caucasus). We also identified other correlated trophic and bioactive effects in the parasite-host relationship. O. laxissima was characterized by a high accumulation tendency for micro- and macroelements, such as K and Ca, and heavy metals, such as Zn, Ni, and Cd. Parasites can reduce the concentration of heavy metals in host tissues owing to this high accumulation tendency. In total, 85 compounds were identified in the examined parasite and its hosts. Despite the distinct phytochemical content of species of the infected host, the parasite produced specific metabolites with dominant phenylethanoid glycosides (PhGs), with acteoside and crenatoside being the primary dominant compounds - ca. 98% of all polyphenols. Polyphenols in parasite specimens that are correlated with Cu and Zn are antagonistic to polyphenols correlated with Fe, Pb, Cr, and Ni. The profile of polyphenols in the host species was both qualitatively and quantitatively distinct from the profile of the compounds in the parasite and between hosts (only acteoside in group PhGs was common between the parasite and Fraxinus host), which indicates the existence of a unique compound biosynthesis pathway in the parasite. Our results demonstrated that the parasite, particularly in its flowers, exhibited higher polyphenol content, antioxidative effects (ABTS-+, DPPH, and FRAP), and inhibitory effects.
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http://dx.doi.org/10.1016/j.phytochem.2021.112844DOI Listing
October 2021

Threatened Masculinity: Gender-Related Collective Narcissism Predicts Prejudice Toward Gay and Lesbian People among Heterosexual Men in Poland.

J Homosex 2022 Jun 30;69(7):1222-1237. Epub 2021 Apr 30.

Faculty of Psychology, University of Warsaw, Warsaw, Poland.

We examined the relationships between different types of in-group positivity and prejudice toward gay and lesbian people among heterosexual men. We assumed that prejudice toward gay and lesbian people among heterosexual men should be predicted by gender-related collective narcissism (i.e., an unrealistic belief about in-group's greatness which is contingent on external validation and extremely sensitive to any signs of threats) and not secure gender in-group identification (i.e., a confidently held in-group evaluation which is independent of the recognition of the group by others and serves as a buffer against threats). Across two studies (final s = 212 and 180) we found that gender-related collective narcissism (but not secure gender in-group identification or gender self-esteem) is positively related to prejudice toward gay and lesbian people among heterosexual men. The results of Study 2 demonstrated that this relationship was largely accounted for by the perceived out-group threat.
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http://dx.doi.org/10.1080/00918369.2021.1907067DOI Listing
June 2022

An Experiencer, An Animal or An Object? Erection Salience Decreases Men's Perceived Agency.

Arch Sex Behav 2020 11 7;49(8):2993-3003. Epub 2020 Sep 7.

Faculty of Psychology, University of Warsaw, ul. Stawki 5/7, 00-183, Warsaw, Poland.

Three experiments investigated the influence of penile erection on ascriptions of mental capabilities to men. Drawing on sexual objectification literature and the distinction between agency and experience in mind perception, three competing predictions were formulated. The mind redistribution hypothesis assumed that penile erection would lower agency and heighten experience attributions, the animalistic dehumanization hypothesis predicted the decrease in agency, but not experience, and the literal objectification hypothesis implied the simultaneous decrease in both agency and experience. In Experiment 1 (N = 219; 128 females), erection salience lowered agency, but not experience capabilities ascribed to male targets. Experiment 2 (N = 201, 113 females) replicated the negative effect of erection salience on perceived agency (but not experience) and revealed that erection salience lowered intentions to hire a male target. This effect was explained with the loss of perceived agency. Experiment 3 (N = 203, 98 females) verified the causal relationship between penile erection, agency and hiring intentions. Taken together, these results supported the animalistic dehumanization hypothesis.
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http://dx.doi.org/10.1007/s10508-020-01800-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7641924PMC
November 2020

Antioxidative assessment of new trans-palladium (II) complexes in head and neck cancer.

Otolaryngol Pol 2019 Aug;74(1):1-6

Klinika Otolaryngologii, Onkologii Laryngologicznej, Audiologii i Foniatrii, II Katedry Otolaryngologii Uniwersytetu Medycznego w Łodzi.

Background: Head and neck neoplasms stand for 6% of all malignant neoplasms worldwide. Chemotherapy has limited use due to the biological properties of the tumor (in the majority of cases moderately and poorly differentiated squamous cell carcinoma). The fundamental molecule used in treatment is cisplatin and its derivates, that can be associated with fluorouracil. The new chemotherapeutic agents are not in common use during the treatment of head and neck malignancies. However, the use of low molecular weight complexes Pd (II) carries the potential of being more effective in therapy.

Material And Methods: Fifty-one patients, 30 men and 21 women (aged 52.9 ± 12.1 years) with head and neck cancer were included in the study. Fifty-one healthy subjects, 31 men and 20 women, (aged 54.1 ± 14.7 years) years formed the control group. Antioxidant enzymes, superoxide dismutase, and catalase activities in erythrocytes were examined.

Results: An increased level of antioxidant enzymes was seen in the blood samples from patients with head and neck cancer after incubation with Pd (II) complex. In the group we obtained a statistically significant result p = <0.001.

Discussion: That project may contribute to the development of new, more efficient head and neck cancer treatment strategies. In our opinion, the results can be used in the future to develop a valuable prognostic marker of the disease. This is important because the initial phase of cancer is asymptomatic. The search for factors involved in pathogenesis translates into economic benefits and makes therapy more effectiveness through the reduction of treatment expenses.
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http://dx.doi.org/10.5604/01.3001.0013.3216DOI Listing
August 2019

A Summary of In Vitro and In Vivo Studies Evaluating the Impact of E-Cigarette Exposure on Living Organisms and the Environment.

Int J Mol Sci 2020 Jan 19;21(2). Epub 2020 Jan 19.

Department of Economic Informatics, University of Lodz, 90-214 Lodz, Poland.

Worldwide use of electronic cigarettes has been rapidly expanding over recent years, but the long-term effect of e-cigarette vapor exposure on human health and environment is not well established; however, its mechanism of action entails the production of reactive oxygen species and trace metals, and the exacerbation of inflammation, which are associated with potential cytotoxicity and genotoxicity. The present study examines the effects of selected liquid chemicals used in e-cigarettes, such as propylene glycol/vegetable glycerin, nicotine and flavorings, on living organisms; the data collected indicates that exposure to e-cigarette liquid has potentially detrimental effects on cells in vitro, and on animals and humans in vivo. While e-liquid exposure can adversely influence the physiology of living organisms, vaping is recommended as an alternative for tobacco smoking. The study also compares the impact of e-cigarette liquid exposure and traditional cigarette smoke on organisms and the environmental impact. The environmental influence of e-cigarette use is closely connected with the emission of airborne particulate matter, suggesting the possibility of passive smoking. The obtained data provides an insight into the impact of nicotine delivery systems on living organisms and the environment.
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http://dx.doi.org/10.3390/ijms21020652DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7013895PMC
January 2020

Plant Extracts and Reactive Oxygen Species as Two Counteracting Agents with Anti- and Pro-Obesity Properties.

Int J Mol Sci 2019 Sep 14;20(18). Epub 2019 Sep 14.

Department of Economic Informatics, University of Lodz, 90-214 Lodz, Poland.

Obesity is a complex disease of great public health significance worldwide: It entails several complications including diabetes mellitus type 2, cardiovascular dysfunction and hypertension, and its prevalence is increasing around the world. The pathogenesis of obesity is closely related to reactive oxygen species. The role of reactive oxygen species as regulatory factors in mitochondrial activity in obese subjects, molecules taking part in inflammation processes linked to excessive size and number of adipocytes, and as agents governing the energy balance in hypothalamus neurons has been examined. Phytotherapy is the traditional form of treating health problems using plant-derived medications. Some plant extracts are known to act as anti-obesity agents and have been screened in in vitro models based on the inhibition of lipid accumulation in 3T3-L1 cells and activity of pancreatic lipase methods and in in vivo high-fat diet-induced obesity rat/mouse models and human models. Plant products may be a good natural alternative for weight management and a source of numerous biologically-active chemicals, including antioxidant polyphenols that can counteract the oxidative stress associated with obesity. This review presents polyphenols as natural complementary therapy, and a good nutritional strategy, for treating obesity without serious side effects.
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http://dx.doi.org/10.3390/ijms20184556DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6770307PMC
September 2019

Practical Aspects of Hyaluronic Acid Application in Biolevox HA Preparations - Report on Research.

Ortop Traumatol Rehabil 2018 Oct;20(5):431-435

Wyższa Szkoła Biznesu i Nauk o Zdrowiu w Łodzi, Polska / The Academy of Business and Health Sciences of Lodz, Poland.

HA preparations in intraarticular injections have been applied in medicine since 1986 and became widely used in Poland at the beginning of the 90s of the twentieth century. Since then, numerous cases of successful application of intra-articular injections (the so called viscosupplementation) have been described in Polish and foreign literature. Changes in the molecular mass of hyaluronic acid and the related specific spatial structure result in different metabolic and immunological properties. Presently, in clinical practice, it is indicated to use the preparations produced by selected manufacturers who offer different forms of HA, containing different percentage of active substance (1 to 2.5%) and different molecular mass, making the desired number (1-5) of intra-articular injec-tions possible, depending on the type and intensity of a specific disease unit. Numerous studies have been con-ducted to confirm the effectiveness of treatment using hyaluronic acid. In Poland a research program was implemented to assess the practical application of 2 ml of Biolevox HA preparation in 2.2% concentration. During the study 15,000 patients were subjected to analysis. The physicians participating in the study injected the hyaluronic acid, in that case, Biolevox HA, to the knee joint, in patients with osteoarthritis, chondromalacia, knee joint injuries and other conditions.
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http://dx.doi.org/10.5604/01.3001.0012.7474DOI Listing
October 2018

The relationship between chest pain and level of perioperative anxiety in patients with lung cancer.

Pol Przegl Chir 2018 Apr;90(2):23-27

Introduction: Chest pain is one of the symptoms of lung cancer.Chest pain disrupts patient's functioning in somatic and psychic area.

Purpose: Whether the existence of chest pain affects the level of perioperative anxiety in lung cancer patients. Is there a relationship between everyday functioning due to the chest pain and the level of perioperative anxiety? Is there a relation between anxiety associated with pain and gender? What is the cause of anxiety in this group of the patiens?

Methodology: The study was conducted among 150 patients with lung cancer before the scheduled surgery. Data was collected with the use of questionnaire assessment of perioperative anxiety level in patients with lung cancer.

Results: The chest pain before the surgery was confirmed by 63 (42%) patients, in case of 87 (58%) patients it was not identified. Chest pain was in case of 48% women and 36% men. 50 (33,3%) research participants who experienced chest pain and 35 patients (23,3%) without this symptom declared experiencing perioperative anxiety. In patients with lung cancer, the correlation: between chest pain and perioperative anxiety was (Z = -4.67; p< .001); between the difficult daily functioning of pain and perioperative anxiety was (Z = -4.72; p< .001); between gender and perioperative anxiety associated with pain was (Z = -3.24; p = 001).Patients afraid of: pain (37,3%), breathing problems (24,0%), physical disability (16,0%), eating problems (12,0%) sleep disorders (9,3%), nothing (1,3%).

Conclusions: Patients with chest pain exhibited significantly higher of perioperative anxiety levels than non-symptomatic patients, as well as higher anxiety levels due to deteriorated daily functioning caused by the disorder. Women had a higher level of anxiety associated with pain than men before the surgery. Patients with lung cancer were most afraid of pain in the perioperative period.
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http://dx.doi.org/10.5604/01.3001.0011.7490DOI Listing
April 2018

An Evaluation of the Antioxidant and Anticancer Properties of Complex Compounds of Copper (II), Platinum (II), Palladium (II) and Ruthenium (III) for Use in Cancer Therapy.

Mini Rev Med Chem 2018 ;18(16):1373-1381

Department of Chemistry and Clinical Biochemistry, Faculty of Medicine, Medical University of Lodz, Lodz, Poland.

The incidence of head and neck cancer increases every year, and represents a significant problem for Molecular Biology at the beginning of the Twenty-first Century. Despite advances in treatment, most studies still strive to deepen the understanding of the mechanisms of cancerogenesis. In order to develop more effective methods of diagnosis and therapy, it is necessary to understand the molecular basis of the neoplastic transformation process. Such findings obtained by research into Molecular Biology, Molecular Epidemiology and Genetics have brought significant progress in understanding the complex, multi- step process which takes place during tumor formation. For many years, there has been great interest in the use of complex compounds, particular complexes between heterocyclic compounds and the metals Pt (II), Pd (II), Cu (II) and Ru (III). One such compound is an azole called pyrazole; the azoles are known to have analgesic, antipyretic, anti-tumor, anti-inflammatory and anti-fungal effects. Improved synthetic and separation methods and biochemical techniques have improved the identification of compounds with biological activity. The main aim of this review is to present complexes with Pt (II), Pd (II), Cu (II) and Ru (III) ions which demonstrate antioxidant and anti-cancer properties.
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http://dx.doi.org/10.2174/1389557518666180423145825DOI Listing
October 2018

Neuropathy - Damage to Peripheral Nerves in Diseases and Injuries of the Musculoskeletal System.

Ortop Traumatol Rehabil 2017 Dec;19(6):569-576

Uniwersytet Medyczny w Łodzi / Medical University of Lodz / Zakład Alergologii i Rehabilitacji Oddechowej, II Katedry Otolaryngologii / Department of Allergology and Respiratory Rehabilitation, 2nd Chair of Otolaryngology.

The musculoskeletal diseases and injuries affect not only the musculoskeletal system itself, but also the closely related structures, such as blood vessels and nerves (both within the central and the peripheral nervous system). If these relationships are neglected or the directed neuroprotective and neuro-regeneration therapy is delayed, the final effect of the treatment is compromised. The aim if this paper is describing the important medical and social problem of peripheral neuropathies from the point of view of epidemiology, etiopathogenesis, imaging diagnostics and complex medical procedures using modern approaches and techniques, based on the available literature.
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http://dx.doi.org/10.5604/01.3001.0010.8073DOI Listing
December 2017

Metal Ion Complexes with Pyrazoles, Aziridines and Diaziridines - Synthesis and Biological Activity.

Curr Med Chem 2019 ;26(4):648-663

Department of Cosmetic Raw Materials Chemistry, Faculty of Pharmacy, Medical University of Lodz, Lodz, Poland.

Heterocyclic compounds containing nitrogen ions, like pyrazoles, aziridines, diaziridines and their metal ion complexes with Cu(II), Zn(II) and Ru(III) and others exhibit a wide range of biological activity, including mainly anti-inflammatory, antioxidant, anticancer, and antimicrobial properties. Biological significance of these molecules and thus their potential use in medicine has driven growing interest into their coordination chemistry. A knowledge of the relationship between the structure of chemical compounds and their activity is needed for the synthesis of the preparations possessing the most beneficial features. The choice of interposed substituents may improve biocidal and antitumor action, reduce the toxicity of the initial substance, or even completely eliminate its adverse effects for healthy tissues. The main aim of this review paper is to present the current state of knowledge concerning the synthesis and biological activity of complexes with small heterocyclic ligands containing transition metal ions.
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http://dx.doi.org/10.2174/0929867325666180221124447DOI Listing
November 2019

Polymorphism of MSH2 Gly322Asp and MLH1 -93G>A in non-familial colon cancer - a case-controlled study.

Arch Med Sci 2017 Oct 3;13(6):1295-1302. Epub 2017 Apr 3.

Department of General and Colorectal Surgery, Medical University of Lodz, Lodz, Poland.

Introduction: Our aim was to determine the effect of the single nucleotide polymorphisms (SNP) -93G>A of the MLH1 gene (rs1800734) and Gly322Asp of the MSH2 gene (rs4987188) on the risk of colon cancer (CC) and identify any relationship with clinical factors.

Material And Methods: The study included 144 unrelated patients with sporadic CC (71 males; mean age: 61.7 ±11 years) and 151 control patients (74 males; mean age: 63 ±11 years). DNA was extracted from peripheral blood lymphocytes, and genotypes were determined by polymerase chain reaction-restriction fragment length polymorphism.

Results: In our population, the homozygous G/G genotype of the -93G>AMLH1 gene increased the risk of sporadic CC (OR = 2.07; 95% CI: 1.11-3.83; < 0.02). For A/G and A/A genotypes, the MLH1-93G>A polymorphism was significantly more common in women ( = 0.034). The SNP demonstrated differences in allele distribution according to the location of the tumor, i.e. right vs. left side ( = 0.014), and disease recurrence ( = 0.022). Significant differences were found in the occurrence of Gly322Asp of with regard to primary and recurrent disease ( = 0.001).

Conclusions: The -93G>A polymorphism plays an important role in evaluating the risk of sporadic CC. It can also be used as an indicator in some patients with left-sided and recurrent tumors. MSH2 Gly322Asp is a potential marker in patients with risk of recurrence.
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http://dx.doi.org/10.5114/aoms.2017.67024DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5701696PMC
October 2017

Effect of copper(II) the activity of glutathione peroxidase in patients with head and neck cancer.

Otolaryngol Pol 2016 Nov;70(6):20-25

Department of Head and Neck Neoplasms Surgery Medical University of Lodz, Poland.

Introduction: Head and neck squamous cell carcinoma (HNSCC) accounts for about 6% of all malignant cancers. In the epidemiology of oral cavity neoplasm, important risk factors include: tobacco smoking, alcohol abuse, bad oral hygiene, papilloma virus infection, riboflavin and iron deficiency.

Objective: The objective of the investigation was a synthesis of Cu(II) complex and the evaluation of antioxidative enzymatic barrier in red blood cells of patients with head and neck tumor as well as in the control group.

Materials And Methods: For the investigation conduction, a consent of Bioethics Committee number RNN/142/09/KB was obtained. Blood for the examination was obtained from the patients of the Dapartment of Head and Neck Neoplasms Surgery Medical University of Łódź. The experiment was conducted on the group of 40 patients with HNSCC and 40 healthy people, using spectrophotometric method, glutathione peroxidase was marked.

Results: The investigation was conducted on the hemolysate obtained from the patients that were divided into two groups - a study group (1 and 2), which consisted of patients diagnosed with head and neck cancer and a control group (1 and 2) - healthy people. A significant statistical result for GPX occurred in control-1 and study-1 group with complex compound Cu(II) (p<0,001).

Conclusions: Presented research prove, that complex compound Cis-dichlorobis(N1-hydroxymethyl-3methylpyrazole-κN2)copper (II) has an impact on the activity of the antioxidative GPX enzyme.
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http://dx.doi.org/10.5604/01.3001.0009.3735DOI Listing
November 2016

Distribution of antimicrobial resistance determinants, virulence-associated factors and clustered regularly interspaced palindromic repeats loci in isolates of Enterococcus faecalis from various settings and genetic lineages.

Pathog Dis 2017 03;75(2)

Department of Molecular Microbiology, National Medicines Institute, 00-725 Warsaw, Poland.

Enterococcus faecalis represents an important factor of hospital-associated infections (HAIs). The knowledge on its evolution from a commensal to an opportunistic pathogen is still limited; thus, we performed a study to characterise distribution of factors that may contribute to this adaptation. Using a collection obtained from various settings (hospitalised patients, community carriers, animals, fresh food, sewage, water), we investigated differences in antimicrobial susceptibility, distribution of antimicrobial resistance genes, virulence-associated determinants and phenotypes, and CRISPR loci in the context of the clonal relatedness of isolates. Bayesian Analysis of Population Structure revealed the presence of three major groups; two subgroups comprised almost exclusively HAI isolates, belonging to previously proposed enterococcal high-risk clonal complexes (HiRECCs) 6 and 28. Isolates of these two subgroups were significantly enriched in antimicrobial resistance genes, presumably produced a polysaccharide capsule and often carried the aggregation substance asa1; distribution of other virulence-associated genes, such as esp and cyl, formation of a biofilm and gelatinase production were more variable. Moreover, both subgroups showed a low prevalence of CRISPR-Cas 1 and 3 and presence of small CRISPR2 variants. Our study confirms the importance of HiRECCs in the population of E. faecalis and their confinement to the hospital settings.
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http://dx.doi.org/10.1093/femspd/ftx021DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5827583PMC
March 2017

Endometrial mRNA expression of prostaglandin synthase enzymes PTGS 2, PTGFS and mPTGES 1 in repeat-breeding cows with cytologically determined endometritis.

Acta Vet Hung 2017 03;65(1):96-104

Department of Animal Reproduction with Clinic, University of Warmia and Mazury , Oczapowskiego 14, 10-719 Olsztyn , Poland.

Little is known about the inflammatory response of the endometrium in repeat-breeding cows with subclinical endometritis (SE). The objective of this study was to evaluate the mRNA expression of prostaglandin-endoperoxide synthase 2 (PTGS 2), prostaglandin F2 synthase (PTGFS) and prostaglandin E2 microsomal synthase 1 (mPTGES 1) in the endometrium of repeat-breeding cows with and without SE. SE was diagnosed cytologically using the cytobrush method, with the threshold being set at 5% polymorphonuclear neutrophils. Biopsy samples were obtained from the endometrium of repeat-breeding cows with SE (n = 10) and without SE (n = 10). The mRNA expression of the synthases was evaluated using qRT-PCR. Significantly higher (P < 0.05) expression of the PTGS 2 gene was detected in the repeat breeders with SE, whereas there was no significant difference in the expression of PTGFS and mPTGES 1 mRNAs between repeatbreeding cows with SE and those without it (P > 0.05). Our study confirms that increased endometrial expression of the PTGS 2 gene is involved in the inflammatory response in repeat breeders.
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http://dx.doi.org/10.1556/004.2017.010DOI Listing
March 2017

TC2 C776G polymorphism studies in patients with oral cancer in the Polish population.

Pol J Pathol 2016;67(3):277-282

Department of Head and Neck Neoplasms Surgery, Medical University of Lodz, Poland.

The first signs of oral cancer may resemble developing infections in the mucous membranes, with throat cancer symptoms being similar to those of upper respiratory tract infections. This greatly hinders rapid diagnosis and treatment. Better knowledge of the changes occurring in the metabolism of folic acid can help in understanding the carcinogenesis affecting DNA methylation and genome stability. Polymorphisms in genes encoding enzymes involved in this pathway may influence enzyme activity and thereby interfere with the concentrations of homocysteine and S-adenosylmethionine, which are important for DNA synthesis and cellular methylation reactions. The aim of the study was to determine the risk of oral cancer associated with the TC2 C776G polymorphism, as determined in 119 patients. Genotypes were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The test genotype was found to correspond to the Hardy-Weinberg (HW) equilibrium (p > 0.05). In our population G/G homozygosity of C776G TC2 gene polymorphism increases the risk of oral cancer; OR (odds ratio): 4.3875; 95% CI (confidence interval): 2.0518-9.319; p = 0.001. Regarding C/G genotype of the C776G TC2 gene, polymorphism also increases the risk of developing this cancer; OR 2.4146 95% CI: 1.2803-4.5541; p = 0.01.
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http://dx.doi.org/10.5114/pjp.2016.63780DOI Listing
June 2017

Self-reported quality of sleep is associated with physical strength among community-dwelling young-old adults.

Geriatr Gerontol Int 2017 Nov 6;17(11):1808-1813. Epub 2017 Jan 6.

Department of Human Health Sciences, Kyoto University Graduate School of Medicine, Kyoto, Japan.

Aim: The present study examined the association between self-reported quality of sleep (QOS) and physical function in community-dwelling young-old adults.

Methods: This cross-sectional study included 737 community-dwelling older adults aged 60 years and older (498 women, 239 men); age 68.3 ± 5.2 years (range 60-79 years). Participants completed a self-administered questionnaire, which included the Pittsburgh Sleep Quality Index, as well as information regarding sociodemographic characteristics, physical activity, smoking and alcohol consumption. The χ -test was used to compare categorical variables stratified by QOS, and the unpaired t-test was used for continuous variables for men and women separately. Furthermore, logistic regression analyses were carried out to verify the association between QOS and physical function measures.

Results: The unpaired t-test showed a significant difference ((P < 0.05) in the Timed Up and Go test and physical strength outcomes, which included: handgrip, quadriceps setting, knee extension and hip flexion strength between the good QOS and poor QOS groups among the men. However, the t-test results for physical function outcomes did not show a significant difference among the women. In the logistic regression model, after adjusting for age, sex and potential confounding factors, handgrip strength remained significantly associated with QOS (OR 0.96, 95% CI 0.92-1.00).

Conclusion: QOS was associated with physical function in young-old adults; those with good QOS were especially more likely to possess stronger grip strengths; however, further research is required to identify the temporal relationship. Geriatr Gerontol Int 2017; 17: 1808-1813.
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http://dx.doi.org/10.1111/ggi.12965DOI Listing
November 2017

Association between physical function and the load pattern during stepping-up motion in community-dwelling elderly women.

Arch Gerontol Geriatr 2016 Sep-Oct;66:205-10. Epub 2016 Jun 17.

Human Health Sciences, Graduate School of Medicine, Kyoto University, 53 Shogoin Kawaramachi, Sakyo-ku, Kyoto 606-8507, Japan.

Objective: Stepping-up motion is challenging task for elderly people in daily life. The present study investigated the relationship between the load pattern during stepping-up motion at maximum speed and physical function in elderly women.

Methods: The subjects comprised 109 community-dwelling elderly women (age 72.5±5.3years). The load pattern (maximum load, rate of load production, and stepping-up time) during ascending a 30cm step at maximum speed was measured, using a step up platform that measures the load at the lower and upper level. Physical function, including hip and knee extensor strength and performance on the vertical jump test, one-legged stance test, timed "Up & Go" (TUG) test, and stepping test were measured.

Results: Pearson's correlation analysis showed that stepping-up time was correlated with the maximum load at the lower level (r=-0.51), but not with the maximum load at the upper level. A multiple regression analysis showed that hip extensor strength and performance on the vertical jump, TUG, and stepping tests were significant determinants of the load pattern during stepping-up motion in the elderly women.

Conclusions: Our study revealed that rapid stepping-up ability was more closely related to the maximum load during push-off at the lower level rather than that during weight loading on the upper level, and that the load pattern during stepping-up motion in elderly women was associated with various physical functions such as the hip extensor strength, leg muscle power, dynamic balance function, and agility.
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http://dx.doi.org/10.1016/j.archger.2016.06.005DOI Listing
July 2017

Are there different factors affecting walking speed and gait cycle variability between men and women in community-dwelling older adults?

Aging Clin Exp Res 2017 Apr 11;29(2):215-221. Epub 2016 Apr 11.

Human Health Science, Kyoto University Graduate School of Medicine, 53 Kawahara-cho, Shogoin, Sakyo, Kyoto, 606-8507, Japan.

Background: Few studies have examined the relationships between walking speed and gait cycle variability, and muscle strength and postural stability, with a focus on gender differences.

Aim: The aim of this study was to examine whether there are different factors affecting walking speed and gait cycle variability between men and women in community-dwelling older adults.

Methods: The subjects comprised 712 community-dwelling older adults (252 men, 460 women, aged 68.7 ± 4.8 years). Walking speed and coefficient of variation (CV) of step time at a comfortable walking pace were measured. The maximal isometric strength of six lower limb muscles and postural stability were evaluated. Stepwise regression analysis was performed, using lower limb muscle strength and postural stability as independent variables, to investigate the association with walking speed or CV.

Results: For older men, age, body mass index (BMI) and quadriceps setting (QS) strength were significant and independent determinants of walking speed. No variables were identified as significant determinants of CV. For older women, BMI and hip flexion, hip abduction, QS muscle strength were significant determinants of walking speed. Only hip abduction strength was a significant determinant of CV.

Discussion: The results of this study suggest that QS strength is related to walking speed in both men and women, whereas hip flexion and abduction muscle strength are related to walking speed, and hip abduction muscle strength is related to gait cycle variability in older women.

Conclusion: Gender differences exist in factors affecting walking speed and gait cycle variability in community-dwelling older adults.
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http://dx.doi.org/10.1007/s40520-016-0568-8DOI Listing
April 2017

Evaluation of Patient Feedback following Laparoscopic Cholecystectomy based on Information Described in the Informed Consent form Developed by the Association of Polish Surgeons.

Pol Przegl Chir 2015 Nov;87(11):558-64

Unlabelled: The cholecystectomy procedure is the most routinely performed intervention in general surgery. The current international gold standard is via the laparoscopic approach. It is a safe, minimally-invasive procedure; however, it is associated with complications in 1% of cases. The aim of the study was to analyze patient feedback, by means of a survey, to determine how much knowledge patients possessed about their disease state and proposed surgical intervention, based primarily on information contained within the informed consent form developed by the Association of Polish Surgeons.

Material And Methods: This study involved the participation of 51 patients who underwent laparoscopic cholecystectomy, indicated by a diagnosis of gallstones, in the years 2014 and 2015.

Results: Despite having signed the informed consent form, there was considerable variation among the responses given to the survey by the 51 patients in this study. Some patients' responses were tangential to the questions asked; many patients did not respond to any of the sub points.

Conclusions: Given that this study is based on a small sample size of patients, it must be presumed that the process by which the patient declares his or her informed consent requires further consideration with respect to the means by which it is obtained. The authors of this study thus recommend that multimedia resources be harnessed as part of the process of obtaining the informed consent of patients prior to surgical intervention.
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http://dx.doi.org/10.1515/pjs-2016-0003DOI Listing
November 2015

The role of Cat -262C/T, GPX1 Pro198Leu and Sod1+35A/C gene polymorphisms in a development of primary open-angle glaucoma in a Polish population.

Pol J Pathol 2016;67(4):404-410

The development of glaucoma may be connected with a long-term exposure to oxidative stress caused by free radical (ROS). The main aim of this work was an analysis of associations of Cat-262C/T, GPX1 Pro198Leu and SOD1 35 A/C gene polymorphisms of antioxidant enzymes with a risk of open-angle glaucoma (POAG) in a Polish population. DNA samples collected from 209 patients with POAG and 191 healthy controls were used in this study. Polymorphisms were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). We found that the +35A/C polymorphisms of SOD1 were not associated with a risk of POAG. We found that C/T genotype (1-GPX1Pro198Leu, 2-Cat C262T) is associated with increased risk of open angle glaucoma (1-OR = 2.24; 95% CI: 1.46-3.44; p = 0.0001, 2-OR = 2.16; 95% CI: 1.35-3.34; p = 0.001). We also found that T/T genotype is a risk factor for progression of POAG (1-OR = 3.86; 95% Cl: 1.36-10.96; p = 0.007, 2-OR = 6.37; 95% CI: 1.39-29.28; p = 0.007). Finally our data suggest that gene polymorphisms of GPX1 Pro198Leu and CAT C262T may have a protective role in the development of primary open-angle glaucoma in a Polish population.
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http://dx.doi.org/10.5114/pjp.2016.65875DOI Listing
July 2017

Evaluation Of Antioxidant Defense In Patients With Colorectal Carcinoma.

Pol Przegl Chir 2015 Jul;87(7):357-61

Unlabelled: Cancers are among the most feared diseases of modern civilization. In Poland, colorectal cancer is one of the tumors with the worst prognosis. The ability to cure is primarily dependent on the stage of the disease at the time of diagnosis. The aim of the study was evaluate antioxidant response in patients with colorectal carcinoma.

Material And Methods: Twenty patients (14 men and 6 women) aged 61.9± 11.1 years with colorectal cancer were included in the study. Twenty healthy subjects (4 men and 16 women) aged 64 ± 15.3 years formed the control group. The erythrocyte activities of antioxidant enzymes, superoxide dismutase (SOD), and glutathione peroxidase (GPx), Results. A significant increase of GPx, and SOD (p < 0.05) were seen in patients compared to healthy controls.

Conclusion: The results indicate that the tested antioxidant enzyme activity of glutathione peroxidase and superoxide dismutase is increased in patients diagnosed with colorectal cancer compared to the control group.
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http://dx.doi.org/10.1515/pjs-2015-0069DOI Listing
July 2015

Evaluation of the impact of radical tumor resection in lung cancer patients on the activity of selected antioxidant enzymes.

Kardiochir Torakochirurgia Pol 2014 Dec 30;11(4):414-20. Epub 2014 Nov 30.

Military Medical Academy University Teaching Hospital - Central Veterans' Hospital in Łódź, Łódź, Poland.

Introduction: Oxidative stress appears to play an essential role as a secondary messenger in such physiological processes as apoptosis and survival as well as in proliferative signaling pathways. Oxidative damage is also considered to play a pivotal role in ageing, several degenerative diseases, and carcinogenesis. Lung cancer is the most common type of cancer, resulting in over 1.3 million deaths each year worldwide.

Aim: The aim of this study was to evaluate the activity of selected antioxidative enzymes in patients with lung cancer before and after radical surgery.

Material And Methods: The study included 20 patients in the study group and 10 healthy volunteers in the control group. In the study group, blood samples were collected twice: one day before and one day after the operation. In the control group, blood samples were collected once. We estimated the activity of catalase (CAT), glutathione peroxidase (GPX), and superoxide dismutase (SOD) in erythrocytes. The total antioxidant status in blood plasma was also determined.

Results: The activity of the selected antioxidative enzymes was greater in the case of GPX and CAT after surgery; the results were statistically significant in the study group. The activity of SOD remained at a comparable level. The total antioxidant status also increased after surgery in the study group in comparison to its preoperative level.
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http://dx.doi.org/10.5114/kitp.2014.47342DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4349034PMC
December 2014

Effect of self-reported quality of sleep on mobility in older adults.

Geriatr Gerontol Int 2016 Feb 17;16(2):266-71. Epub 2015 Feb 17.

Department of Human Health Sciences, Kyoto University Graduate School of Medicine, Kyoto, Japan.

Aim: Quality of sleep (QOS) has been shown to have an affect on mental and physical well-being, and quality of life. However, few studies have investigated how sleep affects mobility in older adults. The aim of the present study was to examine the relationship between QOS and mobility disorder (MD) in community-dwelling older adults.

Methods: Data collected through self-administered questionnaires was analyzed for 4564 Japanese older adults. Sleep outcomes included two self-reported questions regarding QOS. Logistic regression analysis was used to analyze the association between sleep and MD both by the QOS questions independently and grouped together by the two QOS questions into three groups: often sleeps well and good awakening (GG), does not often sleep well and bad awakening (BB) and a group with one of each (GB/BG).

Results: After adjustment for age, sex and depression, both those who did not often sleep well and those with poor awakening were more likely to show MD (OR 1.45, 95% CI 1.29-1.75), (OR 1.77, 95% CI 1.50-2.08), respectively. When grouped by both questions, those in the BB group had the least favorable outcome with increased odds ratio for possessing a MD (OR 1.95, 95% CI 1.61-2.37), compared with GB group (OR 1.40, 95% CI 1.18-1.67).

Conclusions: Often sleeping well and good morning awakening were both associated with MD, suggesting that self reported QOS is associated with MD. Further research is required to investigate whether improving sleep quality could be a possible means of preventing and reversing MD in older adults.
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http://dx.doi.org/10.1111/ggi.12468DOI Listing
February 2016

Biological properties of benzopyran-based platinum (II) complexes.

Pol Przegl Chir 2014 Apr;86(4):172-6

Unlabelled: The aim of the study was to analyze the physicochemical synthesized complex 3 [(1,3- thiazol -2- ylimino) methyl)]-4H- chromene -4 -one with tetrachloroplatinate(II) dipotassium and determination peroxidase activity and glutathione (GPX) in red blood cells of cancer patients and healthy subjects.

Materials And Methods: Tests were carried out with the approval of the Bioethics Committee No. RNN/260/08/KB. Blood was collected into tubes with anticoagulant (heparin lithium). Determination of glutathione peroxidase activity was performed by methods of Little and O'Brien in 20 person groups hospitalized at the Department of General and Colorectal Surgery Veterans General Hospital in Łódź.

Results: The study was an increase of activity in the control without the compound and after the introduction of the complex relative to the treatment groups. In healthy subjects, without the use of glutathione peroxidase complex averaged 73.25 ± 23.88 U / g Hb after application of the compound corresponds to the reference group 81.01 ± 25.94 U / g Hb. In contrast, in patients without the use of the complex activity amounted to 42.85 ± 27.49 U / g Hb. In the study group, which uses synthesized complex GPX activity corresponds to 67.72 ± 13.44 U / g Hb.

Conclusions: The obtained results underline that the introduction of significant blood antioxidant complex research has a significant impact on the results of the determinations. Statistically significant (p < 0.05) difference occurred in both test and no relation to the administration of the complex in relation to the control of 1. 2.
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http://dx.doi.org/10.2478/pjs-2014-0031DOI Listing
April 2014
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