Publications by authors named "Kang Yang"

454 Publications

Strong Field Enhancement and Unidirectional Scattering Based on Asymmetric Nanoantenna.

Nanomaterials (Basel) 2022 Jun 16;12(12). Epub 2022 Jun 16.

School of Electronic Information Engineering, China West Normal University, Nanchong 637001, China.

Dielectric-metal nanostructures have lately emerged as one of the most promising approaches to modulating light at the optical frequency. Their remarkable electric and magnetic resonances give them a one-of-a-kind ability to augment local field enhancements with negligible absorption losses. Here, we propose a hybrid metal-dielectric-metal (MDM) nanoantenna that contains a dimer of three-layers of shell nanoparticles. In addition, we only theoretically and numerically show the optical properties of the hybrid dimer nanoantenna. We found that the nanoantenna sustained unidirectional forward scattering with narrow beamwidth (30.9 deg) and strong scattering intensity (up to 5 times larger than the single MDM particle). Furthermore, when the hybrid asymmetric dimer was excited by the plane wave with different electric polarization directions, our findings revealed that the hybrid nanoantenna boosted the gap's electric near-field while also supporting unidirectional forward scattering. Finally, we analyzed the hybrid dimer with substrates of different materials. It supported strong electric high-order moments along the z-axis and x-axis in gaps between MDM nanoparticles and between MDM nanoparticles and the Ge substrate, owing to the intense displacement currents inside of the dielectric layer. We found that the local electric field of this MDM hybrid dimer nanoantenna with Ge substrate was well improved and attained 3325 v/m.
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http://dx.doi.org/10.3390/nano12122084DOI Listing
June 2022

Contributions of Red Blood Cell Sedimentation in a Driving Syringe to Blood Flow in Capillary Channels.

Authors:
Yang Jun Kang

Micromachines (Basel) 2022 Jun 8;13(6). Epub 2022 Jun 8.

Department of Mechanical Engineering, Chosun University, 309 Pilmun-daero, Dong-gu, Gwangju 61452, Korea.

The erythrocyte sedimentation rate (ESR), which has been commonly used to detect physiological and pathological diseases in clinical settings, has been quantified using an interface in a vertical tube. However, previous methods do not provide biophysical information on blood during the ESR test. Therefore, it is necessary to quantify the individual contributions in terms of viscosity and pressure. In this study, to quantify RBC sedimentation, the image intensity () and interface () were obtained by analyzing the blood flow in the microfluidic channels. Based on threshold image intensity, the corresponding interfaces of RBCs ( > 0.15) and diluent ( < 0.15) were employed to obtain the viscosities (, ) and junction pressures (, ). Two coefficients (, ) obtained from the empirical formulas ( [1 + ], [1 + ]) were calculated to quantify RBC sedimentation. The present method was then adopted to detect differences in RBC sedimentation for various suspended blood samples (healthy RBCs suspended in dextran solutions or plasma). Based on the experimental results, four parameters (, , , and ) are considered to be effective for quantifying the contributions of the hematocrit and diluent. Two coefficients exhibited more consistent trends than the conventional ESR method. In conclusion, the proposed method can effectively detect RBC sedimentation.
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http://dx.doi.org/10.3390/mi13060909DOI Listing
June 2022

Poly(β-cyclodextrin)/platinum prodrug supramolecular nano system for enhanced cancer therapy: Synthesis and in vivo study.

Carbohydr Polym 2022 Sep 4;292:119695. Epub 2022 Jun 4.

School of Biomedical Engineering; State Key Laboratory of Oncology in South China, Sun Yat-sen University, Guangzhou 510006, China. Electronic address:

The use of cisplatin is restricted by systemic toxicity and drug resistance. Supramolecular nano-drug delivery systems involving drugs as building blocks circumvent these limitations promisingly. Herein, we describe a novel supramolecular system [Pt(IV)-SSNPs] based on poly(β-cyclodextrin), which was synthesized for efficient loading of adamantly-functionalized platinum(IV) prodrug [Pt(IV)-ADA] via the host-guest interaction between β-cyclodextrin and adamantyl. Pt(IV)-ADA can be converted to active cisplatin in reducing environment in cancer cells, which further reduces systemic toxicity. The introduction of the adamantane group-tethered mPEG endowed the Pt(IV)-SSNPs with a longer blood circulation time. In vitro assays exhibited that the Pt(IV)-SSNPs could be uptaken by CT26 cells, resulting in cell cycle arrest in the G2/M and S phases, together with apoptosis. Furthermore, the Pt(IV)-SSNPs showed effective tumor accumulation, better antitumor effect, and negligible cytotoxicity to major organs. These results indicate that supramolecular nanoparticles are a promising platform for efficient cisplatin delivery and cancer treatment.
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http://dx.doi.org/10.1016/j.carbpol.2022.119695DOI Listing
September 2022

GGC repeat expansion of RILPL1 is associated with oculopharyngodistal myopathy.

Ann Neurol 2022 Jun 14. Epub 2022 Jun 14.

Department of Neurology and Institute of Neurology of First Affiliated Hospital, Institute of Neuroscience, and Fujian Key Laboratory of Molecular Neurology, Fujian Medical University, Fuzhou, 350005, China.

Objective: Oculopharyngodistal myopathy (OPDM) is an adult-onset neuromuscular disease characterized by progressive ptosis, dysarthria, ophthalmoplegia, and distal muscle weakness. Recent studies revealed GGC repeat expansions in 5'-UTR of LRP12, GIPC1, and NOTCH2NLC are associated with OPDM. Despite these advances, around 30% of OPDM patients remain genetically undiagnosed. Herein, we aim to investigate genetic basis for undiagnosed OPDM patients in two unrelated Chinese Han families.

Methods: Parametric linkage analysis was performed. Long-read sequencing followed by repeat-primed polymerase chain reaction (RP-PCR) and amplicon length polymerase chain reaction (AL-PCR) were used to determine the genetic cause. Targeted methylation sequencing was implemented to detect epigenetic changes. The possible pathogenesis mechanism was investigated by qPCR, immunoblotting, RNA FISH, and immunofluorescence staining of muscle biopsy samples.

Results: The disease locus was mapped to 12q24.3. Subsequently, GGC repeat expansion in the promoter region of RILPL1 was identified in six OPDM patients from two families, findings consistent with a founder effect, designated as OPDM type 4 (OPDM4). Targeted methylation sequencing revealed hypermethylation at RILPL1 locus in unaffected individuals with ultralong expansion. Analysis of muscle samples showed no significant differences in RILPL1 mRNA or RILPL1 protein levels between patients and controls. Public CAGE-seq data indicated that alternative TSSs exist upstream of the RefSeq-annotated RILPL1 TSS. Strand-specific RNAseq data revealed bidirectional transcription from the RILPL1 locus. Finally, FISH/IF indicated that both sense and antisense transcripts formed RNA foci and were co-localized with hnRNPA2B1 and p62 in the intranuclear inclusions of OPDM4 patients.

Interpretation: Our findings implicate abnormal GGC repeat expansions in the promoter region of RILPL1 as a novel genetic cause for OPDM, and suggest a methylation mechanism and a potential RNA toxicity mechanism are involved in OPDM4 pathogenesis. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/ana.26436DOI Listing
June 2022

Effects of Underground Coal Mining on Soil Spatial Water Content Distribution and Plant Growth Type in Northwest China.

ACS Omega 2022 Jun 23;7(22):18688-18698. Epub 2022 May 23.

School of Chemical &Environmental Engineering, China University of Mining and Technology, Beijing 100083, China.

The impact of coal mining subsidence on surface ecology involves the influence of several ecological elements such as water, soil, and vegetation, which is systematic and complex. Given the unclear understanding of the synergistic change patterns of the water-soil-vegetation ecological elements in the influence of coal mining in the west, this paper investigates the impact of coal mining on the surface ecology, especially the distribution of soil water content (SWC). In 2020, this study collected 3000 soil samples from 60 sampling points (at depth of 0-10 m) and tested the SWC. All samples come from three different temporal and spatial areas of coal mining subsidence in the desert mining area of Northwest China where soil types are mainly aridisols. At the same time, the interactions among deep SWC and surface soil physical and chemical properties, surface SWC and soil fertility, and pH were analyzed. The spatial variability of soil moisture is reflected by kriging interpolation, and SWC values at different depths are predicted as a basis for monitoring the environmental impact of different coal mining subsidence years. The research has shown that the ground subsidence leads to a decrease in SWC value and changes in surface soil pH, physical and chemical properties, and covering vegetation, which have occurred from the beginning of coal mining. The impact of coal mining on the SWC of the unsaturated zone is mainly at the depth of 0-6 m, where SWC is not directly related to the nutrient content of the surface soil. The overall settlement of the ground will stir up simultaneous decline in the quality of deep SWC and topsoil. The findings of this investigation suggest that changes in the soil structure caused by coal mining subsidence are the key factor in SWC loss. Timely monitoring and repairing 0-6 m ground fissures, as well as selecting shrubs on the surface is the best choice for the restoration of the ecological environment and prevention of soil erosion in this area.
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http://dx.doi.org/10.1021/acsomega.2c01369DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9178752PMC
June 2022

Ongoing Clinical Trials in Aging-Related Tissue Fibrosis and New Findings Related to AhR Pathways.

Aging Dis 2022 Jun 1;13(3):732-752. Epub 2022 Jun 1.

1Department of Nephrology, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin, China.

Fibrosis is a pathological manifestation of wound healing that replaces dead/damaged tissue with collagen-rich scar tissue to maintain homeostasis, and complications from fibrosis contribute to nearly half of all deaths in the industrialized world. Ageing is closely associated with a progressive decline in organ function, and the prevalence of tissue fibrosis dramatically increases with age. Despite the heavy clinical and economic burden of organ fibrosis as the population ages, to date, there is a paucity of therapeutic strategies that are specifically designed to slow fibrosis. Aryl hydrocarbon receptor (AhR) is an environment-sensing transcription factor that exacerbates aging phenotypes in different tissues that has been brought back into the spotlight again with economic development since AhR could interact with persistent organic pollutants derived from incomplete waste combustion. In addition, gut microbiota dysbiosis plays a pivotal role in the pathogenesis of numerous diseases, and microbiota-associated tryptophan metabolites are dedicated contributors to fibrogenesis by acting as AhR ligands. Therefore, a better understanding of the effects of tryptophan metabolites on fibrosis modulation through AhR may facilitate the exploitation of new therapeutic avenues for patients with organ fibrosis. In this review, we primarily focus on how tryptophan-derived metabolites are involved in renal fibrosis, idiopathic pulmonary fibrosis, hepatic fibrosis and cardiac fibrosis. Moreover, a series of ongoing clinical trials are highlighted.
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http://dx.doi.org/10.14336/AD.2021.1105DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9116921PMC
June 2022

Design and synthesis of highly TRAIL expression HDAC inhibitors based on ONC201 to promote apoptosis of colorectal cancer.

Eur J Med Chem 2022 Aug 27;238:114484. Epub 2022 May 27.

School of Science, China Pharmaceutical University, 639 Longmian Avenue, Nanjing, 211198, PR China. Electronic address:

Activation of the TRAIL proapoptotic pathway can promote cancer cell apoptosis. Histone deacetylases (HDACs) also are promising drug targets for cancers, and their synergistic effect with TRAIL can improve the inhibitory effect on cancer cells. Therefore, the development of highly TRAIL-sensitive HDAC inhibitors might be a promising strategy for the treatment of cancers. We synthesized a series of HDAC inhibitors by introducing effective fragments sensitive to TRAIL. Compound IIc showed good inhibitory activity against HDAC1 and HCT116 cells and showed higher sensitivity to activating the expression of the TRAIL protein and promoting the apoptosis of HCT-116 cells compared with ONC201. The inhibitory activity of compound IIc (25 mg/kg) in the HCT-116 xenograft model was significantly greater than those of the positive control drugs (ONC201, chidamide). These findings suggested that development of highly TRAIL-sensitive HDAC inhibitors as colorectal tumor cancer drugs.
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http://dx.doi.org/10.1016/j.ejmech.2022.114484DOI Listing
August 2022

Gallnut Tannic Acid Exerts Anti-stress Effects on Stress-Induced Inflammatory Response, Dysbiotic Gut Microbiota, and Alterations of Serum Metabolic Profile in Beagle Dogs.

Front Nutr 2022 27;9:847966. Epub 2022 Apr 27.

Guangdong Laboratory for Lingnan Modern Agriculture, Guangdong Provincial Key Laboratory of Animal Nutrition Control, National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou, China.

Stress exposure is a potential threat to humans who live or work in extreme environments, often leading to oxidative stress, inflammatory response, intestinal dysbiosis, and metabolic disorders. Gallnut tannic acid (TA), a naturally occurring polyphenolic compound, has become a compelling source due to its favorable anti-diarrheal, anti-oxidative, anti-inflammatory, and anti-microbial activities. Thus, this study aimed to evaluate the anti-stress effects of gallnut TA on the stress-induced inflammatory response, dysbiotic gut microbiota, and alterations of serum metabolic profile using beagle models. A total of 13 beagle dogs were randomly divided into the stress (ST) and ST + TA groups. Dietary supplementation with TA at 2.5 g/kg was individually fed to each dog in the ST + TA group for 14 consecutive days. On day 7, all dogs were transported for 3 h from a stressful environment (days 1-7) to a livable site (days 8-14). In our results, TA relieved environmental stress-induced diarrheal symptoms in dogs and were shown to protect from myocardial injury and help improve immunity by serum biochemistry and hematology analysis. Also, TA inhibited the secretion of serum hormones [cortisol (COR), glucocorticoid (GC), and adrenocorticotropic hormone (ACTH)] and the expression of heat shock protein (HSP) 70 to protect dogs from stress-induced injury, thereby relieving oxidative stress and inflammatory response. Fecal 16S rRNA gene sequencing revealed that TA stimulated the growth of beneficial bacteria (, , , and ) and suppressed the growth of pathogenic bacteria ( and ), thereby increasing fecal butyrate levels. Serum metabolomics further showed that phytosphingosine, indoleacetic acid, arachidonic acid, and biotin, related to the metabolism of sphingolipid, tryptophan, arachidonic acid, and biotin, respectively, could serve as potential biomarkers of stress exposure. Furthermore, Spearman's correlation analysis showed strong relationships between the four potential serum biomarkers and differential bacteria. Overall, gallnut TA may be a potential prebiotic for the prevention and treatment of stress-induced metabolic disorders by targeting intestinal microbiota.
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http://dx.doi.org/10.3389/fnut.2022.847966DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9094144PMC
April 2022

Smart dual responsive nanocarriers with reactive oxygen species amplification assisted synergistic chemotherapy against prostate cancer.

J Colloid Interface Sci 2022 Sep 2;622:789-803. Epub 2022 May 2.

Department of Urology, Kidney and Urology Center, Pelvic Floor Disorders Center, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen 518107, China. Electronic address:

Conventional chemotherapy efficacy is impeded by poor water solubility, inferior tissue targeting, and severe systemic toxic effect. Synergistic chemotherapy has become prominent with the stimulus-response drug delivery system (DDS) to treat solid malignancies. The most popularly employed responsive stimulus is reactive oxygen species (ROS), which is proven to primarily sensitize chemotherapy and enhance antitumor impact. In this study, we have successfully developed smart dual responsive nanocarriers with ROS self-amplification, particularly responding to disassemble under the high levels of ROS and esterase in the tumor microenvironment (TME) and to release docetaxel (DTX) efficiently. Additionally, we utilized palmitoyl ascorbate (PA) as a stabilizer by taking advantage of its amphiphilic structure. PA is also an excellent ROS generator that produces a large amount of hydrogen peroxide (HO) in TME to achieve ROS self-amplification. Also, elevated levels of ROS could continue to activate ROS-sensitive thioketal and make the remaining nanocarriers disassemble for sustaining the release of chemotherapeutics, realizing a positive feedback loop for ROS generation and ROS amplification, as well as sensitizing chemotherapy efficacy. The smart dual responsive nanocarriers may serve as a promising and prospective strategy for treating prostate cancer and promoting synergistic cancer therapy.
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http://dx.doi.org/10.1016/j.jcis.2022.04.167DOI Listing
September 2022

Liraglutide Is Protective against Brain Injury in Mice with Febrile Seizures by Inhibiting Inflammatory Factors.

Comput Math Methods Med 2022 29;2022:7563281. Epub 2022 Apr 29.

Department of Pediatrics, The Third Affiliated Hospital of Qiqihar Medical University, Qiqihar 161000, China.

The febrile seizure (FS) is a common disease in emergency pediatrics, and about 30% of patients are children aged between 6 months and 5 years. Therefore, we aim to observe the protective impact of liraglutide (LIR) on brain injury in mice with FS and to explore its relevant mechanisms. Male SD mice were selected, and the FS model was established by heat bath method. The behavioral score was performed on mice with Racine grading, and nerve cells in apoptosis in the hippocampus were determined by TUNEL. The content of glutamate was determined by ELISA. mRNA levels and protein expression of GLP-1, GLP-1R, IL-1, IL-6, TNF-, and cleaved-caspase 3 were examined in mice by q-PCR and WB. Protein expression of -aminobutyric acid was influenced by WB as well. LIR prolonged the seizure latency and seizure duration in mice with FS. The GLP-1 and GLP-1R in the mouse hippocampus with FS expressed highly and also inhibited the number of nerve cells in apoptosis, decreased glutamate content, and increased -aminobutyric acid expression in the mouse hippocampus with FS. In addition, The IL-1, IL-6, and TNF-, in the mouse hippocampus with FS expressed to reduce with LIR. LIR is protective against brain injury in mice with FS and protects brain injury by inhibiting inflammatory factors in mice with FS. Our finding provides a reference for mitigating and delaying the development of FS as well as the prevention and treatment of brain injury caused by FS.
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http://dx.doi.org/10.1155/2022/7563281DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9076292PMC
May 2022

Expanding the phenotype and genotype spectra of PLIN4-associated myopathy with rimmed ubiquitin-positive autophagic vacuolation.

Acta Neuropathol 2022 Jun 2;143(6):733-735. Epub 2022 May 2.

Department of Neurology and Institute of Neurology of First Affiliated Hospital, Institute of Neuroscience, and Fujian Key Laboratory of Molecular Neurology, Fujian Medical University, Fuzhou, 350005, China.

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http://dx.doi.org/10.1007/s00401-022-02422-7DOI Listing
June 2022

Polyhydroxylated eudesmane sesquiterpenoids and sesquiterpenoid glucoside from the flower buds of Tussilago farfara.

Chin J Nat Med 2022 Apr;20(4):301-308

School of Biological Science and Technology, University of Jinan, Jinan 250022, China. Electronic address:

Chemical fractionation of the n-BuOH partition, which was generated from the EtOH extract of the flower buds of Tussilago farfara, afforded a series of polar constituents including four new sesquiterpenoids (1-4), one new sesquiterpenoid glucoside (5) and one known analogue (6) of the eudesmane type, as well as five known quinic acid derivatives (7-11). Structures of the new compounds were unambiguously characterized by detailed spectroscopic analyses, with their absolute configurations being established by X-ray crystallography, electronic circular dichroism (ECD) calculation and induced ECD experiments. The inhibitory effect of all the isolates against LPS-induced NO production in murine RAW264.7 macrophages was evaluated, with isochlorogenic acid A (7) showing significant inhibitory activity.
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http://dx.doi.org/10.1016/S1875-5364(21)60120-6DOI Listing
April 2022

First metatarsophalangeal joint arthrodesis/fusion: a systematic review of modern fixation techniques.

J Foot Ankle Res 2022 Apr 26;15(1):30. Epub 2022 Apr 26.

Department of Podiatry, School of Human and Health Sciences, University of Huddersfield, Huddersfield, UK.

Background: First metatarsophalangeal joint arthrodesis is commonly performed for symptomatic end-stage hallux rigidus. It has been postulated to produce good results in the literature. Various fixation techniques offer differences in union rates, complications and functional outcomes, stirring debates about which produces the best outcomes for patients. Therefore, this review aims to synthesise and compare the outcomes of modern fixation techniques used for first metatarsophalangeal joint (FMPJ) arthrodesis.

Methods: The electronic database searched were PubMed, CINAHL, Cochrane Library, and Google Scholar. The critical appraisal skills programme tool for cohort study was used. The interventions consisted of screw(s), plate(s), and staple(s). Studies comprising outdated fixation techniques such as suture, metallic wire, external fixation, Rush rods or Steinmann pins were excluded. Participants were adults over 18 years, undergoing FMPJ arthrodesis in the UK. Studies with the population consisting primarily of revision cases, patients with rheumatoid arthritis or diabetes were excluded.

Results: Seven UK studies included 277 feet and a 95.7% overall union rate at a mean union time of 83.5 days. Staples had the highest union rate of 98.2% at mean union time of 84 days, followed by plates (95.2%, 92 days), and finally screws (94.9%, 71 days). The overall complication incidence is 5.8%. All of the fixation techniques produced good functional outcomes postoperatively.

Conclusions: Whilst staple techniques showed the highest union rate, plating techniques are preferable over screws or staples for better results across several outcome measures, including reduced complication incidence, stability, early ambulation, and good functional outcome. The Manchester-Oxford Foot Questionnaire and EuroQol-5Dimensional are recommended as measurement tools to assess functional outcomes following FMPJ arthrodesis.
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http://dx.doi.org/10.1186/s13047-022-00540-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9040205PMC
April 2022

Aconitine: A review of its pharmacokinetics, pharmacology, toxicology and detoxification.

J Ethnopharmacol 2022 Jul 8;293:115270. Epub 2022 Apr 8.

The First Affiliated Hospital of Henan University of Traditional Chinese Medicine, Zhengzhou, 450000, China.

Ethnopharmacological Relevance: Aconitine, a C-norditerpenoid alkaloid, derives from many medicinal plants such as Aconitum carmichaelii Debx. (Chinese:), Aconitum kusnezoffii Reichb (Chinese:), which were used to rheumatic fever, painful joints and some endocrinal disorders.

Aims Of The Review: The present paper reviews research progress relating to the pharmacokinetics, physiological and pathological processes of aconitine, while some promising research direction and the detoxification of aconitine are also discussed.

Materials And Methods: The accessible literature on aconitine, from 1990 to 2020, obtained from published materials of electronic databases, such as SCI finder, PubMed, Web of Science, Science Direct, Springer and Google Scholar was systematically analyzed.

Results: In this review, we address the pharmacokinetics of aconitine, as well as its pharmacological effects including anti-cancer, anti-inflammatory, anti-virus, immunoregulation, analgesic, insecticide and inhibition of androgen synthesis. Further, we summarize the toxicity of aconitine such as cardiotoxicity and neurotoxicity, on which we strikingly focus on the ways to reduce the toxicity of aconitine based.

Conclusions: Aconitine plays an vital role in a wide range of physiological and pathological processes and we can reduce the toxicity of aconitine by compatibility and hydrolysis. Although some issues still exist, such as the correlative relationship between the dose and toxicity of aconitine not being clear, our review may provide new ideas for the application of aconitine in the treatment of related diseases.
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http://dx.doi.org/10.1016/j.jep.2022.115270DOI Listing
July 2022

Assessment of Blood Biophysical Properties Using Pressure Sensing with Micropump and Microfluidic Comparator.

Authors:
Yang Jun Kang

Micromachines (Basel) 2022 Mar 13;13(3). Epub 2022 Mar 13.

Department of Mechanical Engineering, Chosun University, 309 Pilmun-daero, Dong-gu, Gwangju 61452, Korea.

To identify the biophysical properties of blood samples consistently, macroscopic pumps have been used to maintain constant flow rates in a microfluidic comparator. In this study, the bulk-sized and expensive pump is replaced with a cheap and portable micropump. A specific reference fluid (i.e., glycerin solution [40%]) with a small volume of red blood cell (RBC) (i.e., 1% volume fraction) as fluid tracers is supplied into the microfluidic comparator. An averaged velocity (<>) obtained with micro-particle image velocimetry is converted into the flow rate of reference fluid () (i.e., = × × <>, : cross-sectional area, = 1.156). Two control variables of the micropump (i.e., frequency: 400 Hz and volt: 150 au) are selected to guarantee a consistent flow rate (i.e., COV < 1%). Simultaneously, the blood sample is supplied into the microfluidic channel under specific flow patterns (i.e., constant, sinusoidal, and periodic on-off fashion). By monitoring the interface in the comparator as well as , three biophysical properties (i.e., viscosity, junction pressure, and pressure-induced work) are obtained using analytical expressions derived with a discrete fluidic circuit model. According to the quantitative comparison results between the present method (i.e., micropump) and the previous method (i.e., syringe pump), the micropump provides consistent results when compared with the syringe pump. Thereafter, representative biophysical properties, including the RBC aggregation, are consistently obtained for specific blood samples prepared with dextran solutions ranging from 0 to 40 mg/mL. In conclusion, the present method could be considered as an effective method for quantifying the physical properties of blood samples, where the reference fluid is supplied with a cheap and portable micropump.
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http://dx.doi.org/10.3390/mi13030438DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8949505PMC
March 2022

Adaptive Responses of Common and Hybrid Bermudagrasses to Shade Stress Associated With Changes in Morphology, Photosynthesis, and Secondary Metabolites.

Front Plant Sci 2022 3;13:817105. Epub 2022 Mar 3.

College of Grassland Science and Technology, Sichuan Agricultural University, Chengdu, China.

Alteration of ploidy in one particular plant species often influences their environmental adaptation. Warm-season bermudagrass is widely used as forage, turfgrass, and ground-cover plant for ecological remediation, but exhibits low shade tolerance. Adaptive responses to shade stress between triploid hybrid bermudagrass cultivars ["Tifdwarf" (TD), "Tifsport" (TS), and "Tifway" (TW)] and tetraploid common bermudagrass cultivar "Chuanxi" (CX) were studied based on changes in phenotype, photosynthesis, and secondary metabolites in leaves and stems. Shade stress (250 luminance, 30 days) significantly decreased stem diameter and stem internode length, but did not affect the leaf width of four cultivars. Leaf length of CX, TD, or TW showed no change in response to shade stress, whereas shade stress significantly elongated the leaf length of TS. The CX and the TS exhibited significantly higher total chlorophyll (Chl), Chl a, carotenoid contents, photosynthetic parameters [PSII photochemical efficiency (Fv/Fm), transpiration rate, and stomatal conductance] in leaves than the TW and the TD under shade stress. The CX also showed a significantly higher performance index on absorption basis (PIABS) in leaf and net photosynthetic rate (Pn) in leaf and stem than the other three cultivars under shade stress. In addition, the TS maintained higher proantho cyanidims content than the TW and the TD after 30 days of shade stress. Current results showed that tetraploid CX exhibited significantly higher shade tolerance than triploid TD, TS, and TW mainly by maintaining higher effective photosynthetic leaf area, photosynthetic performance of PSI and PSII (Pn and Fv/Fm), and photosynthetic pigments as well as lower Chl a/b ratio for absorption, transformation, and efficient use of light energy under shade stress. For differential responses to shade stress among three triploid cultivars, an increase in leaf length and maintenance of higher Fv/Fm, gas exchange, water use efficiency, carotenoid, and proanthocyanidin contents in leaves could be better morphological and physiological adaptations of TS to shade than other hybrid cultivars (TD and TW).
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http://dx.doi.org/10.3389/fpls.2022.817105DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8928391PMC
March 2022

Ellman's method-based determination of acibenzolar-S-methyl in tobacco by transesterification with methanol and SABP2-catalyzed hydrolysis.

Anal Sci 2022 May 17;38(5):749-757. Epub 2022 Mar 17.

School of Biological Science and Technology, University of Jinan, Jinan, 250022, People's Republic of China.

Acibenzolar-S-methyl (ASM) is the most commercially successful biological antibacterial agent used for crop through systemic acquired resistance (SAR). In this study, a reproducible and accurate procedure, based on the spectrophotometric/microplate reader analysis, has been developed to detect ASM in tobacco leaves. This method involves oxidation of methyl mercaptan by the Ellman's reagent 5,5'-dithio-bis (2-nitrobenzoic acid) (DTNB) to form the yellow derivative 5'-thio-2-nitrobenzoic acid (TNB), measurable at 412 nm. Methyl mercaptan can be produced by either the ASM transesterification with methanol or the SA-binding protein 2 (SABP2)-catalyzed ASM hydrolysis. The proportions of methanol, reaction time, temperature, the concentrations of EDTA and DTNB were optimized in a 96-well plate. The calibration curve of ASM was linear over the range of 25.2-315 μg g. The results of the intra- and inter-day accuracy and precision data were within the FDA acceptance criteria. With ASM as substrate, the turnover number of SABP2 was determined, with the k value of 31.1 min using the Michaelis-Menten equation. In tobacco plants treated with 100 μM ASM, it was decreased as time elapsed in treated tobacco, reaching negligible values 72 h after treatment. The optimized method was applied for the determination of ASM transesterification with methanol and the kinetic data determination of SABP2-catalyzed ASM hydrolysis.
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http://dx.doi.org/10.1007/s44211-022-00091-wDOI Listing
May 2022

A high bile acid environment promotes apoptosis and inhibits migration in pancreatic cancer.

Bioengineered 2022 03;13(3):6719-6728

Department of General Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China.

Bile acids, the main organic solutes in bile, have been established to play an important role at physiological concentrations in gastrointestinal metabolism. However, under pathological conditions, such as cholestatic disease, cholestasis can damage hepatocytes/biliary epithelial cells leading to apoptosis or necrosis. Clinically, pancreatic head cancer usually presents with obstructive jaundice and increased serum bile acid levels, suggesting that pancreatic cancer is intricately correlated with a high bile acid environment in the human body. An increasing body of evidence suggests that bile acids are toxic to normal human and colon cancer cells. Nonetheless, the effect of bile acids on the occurrence and development of pancreatic cancer remains a matter of debate. In the present study, to explore the direct effects of high serum concentrations of bile acids on pancreatic cancer and the possible related mechanisms, human pancreatic cancer (PANC-1) cells were subject to different concentrations of bile acid mixtures to assess cell viability and the migration and invasion ability. Besides, we found that a high bile acid environment could inhibit the proliferation and migration of pancreatic cancer cells through ROS(Reactive oxygen species) induction and the EMT(epithelial-mesenchymal transition) pathway, thereby promoting the apoptosis of pancreatic cancer cells. BAs: Bile Acids; EMT: epithelial-mesenchymal transition; FBS: fatal bovine serum;CCK-8: Cell-Counting-Kit-8; ROS: reactive oxygen species; CA: cholic acid; CDCA: chenodeoxycholic acid; GCDCA: Glycochenodeoxycholic acid; PVDF: Poly vinylidene fluoride.
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http://dx.doi.org/10.1080/21655979.2022.2045823DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8973854PMC
March 2022

Sequential quantification of blood and diluent using red cell sedimentation-based separation and pressure-induced work in a microfluidic channel.

Authors:
Yang Jun Kang

Anal Methods 2022 03 24;14(12):1194-1207. Epub 2022 Mar 24.

Department of Mechanical Engineering, Chosun University, 309 Pilmun-daero, Dong-gu, Gwangju, Republic of Korea.

The erythrocyte sedimentation method has been widely used to detect inflammatory diseases. However, this conventional method still has several drawbacks, such as a large blood volume (∼1 mL) and difficulty in continuous monitoring. Most importantly, image-based methods cannot quantify RBC-rich blood (blood) and RBC-free blood (diluent) simultaneously. In this study, instead of visualizing interface movement in the blood syringe, a simple method is proposed to quantify blood and diluent in microfluidic channels sequentially. The hematocrit was set to 25% to enhance RBC sedimentation and form two layers (blood and diluent) in the blood syringe. An air cavity (∼300 μL) inside the blood syringe was secured to completely remove dead volumes (∼200 μL) in fluidic paths (syringe needle and tubing). Thus, a small blood volume ( = 50 μL) suctioned into the blood syringe is sufficient for supplying blood and diluent in the blood channel sequentially. The relative ratio of blood resident time (RBC-to-diluent separation) was quantified using , which was obtained by quantifying the image intensity of blood flow. After the junction pressure () and blood volume () were obtained by analyzing the interface in the coflowing channel, the averaged work ( [Pa mm]) was calculated and adopted to detect blood and diluent, respectively. The proposed method was then applied with various concentrations of dextran solution to detect aggregation-elevated blood. The of blood and diluent exhibited substantial differences with respect to dextran solutions ranging from = 10 to = 40 mg mL. Moreover, did not exhibit substantial differences in blood with > 10 mg mL. The variations in were comparable to those of the previous method based on interface movement in the blood syringe. In conclusion, the could detect blood as well as diluents more effectively than . Furthermore, the proposed method substantially reduced the blood volume from 1 mL to 50 μL.
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http://dx.doi.org/10.1039/d1ay02178hDOI Listing
March 2022

MiRNA-21 Regulates Bronchial Epithelial Cell Proliferation by Activating Tgfβ1/Smad Signaling Pathway and Its Correlation with Asthma Severity in Children.

Iran J Public Health 2021 Oct;50(10):1973-1982

Department of Pediatrics, the Third Affiliated Hospital of Qiqihar Medical University, Qiqihar 161000, China.

Background: This research was designed to probe into the role of miRNA-21 in the pathogenesis of childhood asthma and its correlation with the severity.

Methods: Fifty-four children with bronchial asthma admitted to the Third Affiliated Hospital of Qiqihar Medical University from Jun 2018 to Dec 2019 were included. Forty nine healthy children underwent physical examination at this time period were also enrolled. The miR-21 expression in peripheral blood serum was analyzed by qRT-PCR. The relationship between the expression and severity of asthma in children was explored by Spearman correlation analysis and ROC curve. Bronchial epithelial cell lines were cultured in vitro and divided into blank control group, negative control group and miR-21 inhibition and activation group. The changes of cell proliferation after treatment were detected by CCK-8 test in different groups. The expression of TGF-β1/Smad signaling pathway protein in cells was assessed by Western blot (WB).

Results: Compared with that of healthy children, the miR-21 expression in peripheral blood serum of asthmatic children was higher (<0.001). MiR-21 expression was positively correlated with the severity of illness (r=0.853, <0.001). The results of cell experiments in vitro signified that miR-21 can promote the proliferation of bronchial epithelial cells, and may be involved in regulating the expression of TGF-β1/Smad3 signaling pathway, thus affecting cell proliferation.

Conclusion: miRNA-21 regulates the proliferation of bronchial epithelial cells by activating TGFβ1/Smad signaling pathway. And it is positively correlated with the severity of asthma in children.
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http://dx.doi.org/10.18502/ijph.v50i10.7497DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8819233PMC
October 2021

Highly Unidirectional Radiation Enhancement Based on a Hybrid Multilayer Dimer.

Nanomaterials (Basel) 2022 Feb 21;12(4). Epub 2022 Feb 21.

Key Laboratory of Advanced Perception and Intelligent Control of High-End Equipment, Ministry of Education, College of Electrical Engineering, Anhui Polytechnic University, Wuhu 241000, China.

Dimers made of plasmonic particles support strong field enhancements but suffer from large absorption losses, while low-loss dielectric dimers are limited by relatively weak optical confinement. Hybrid dimers could utilize the advantages of both worlds. Here, we propose a hybrid nanoantenna that contains a dimer of core-dual shell nanoparticles known as the metal-dielectric-metal (MDM) structure. We discovered that the hybrid dimer sustained unidirectional forward scattering, which resulted in a nearly ideal Kerker condition in the frequency close to the resonance peak of the dimer due to enhancing the amplitude of the induced high-order electric multiples in the gap and effectively superimposing them with magnetic ones, which respond to the excitation of the plane wave in the dielectric layer of the dimer. Furthermore, when an electric quantum emitter is coupled to the dimer, our study shows that the optimal hybrid dimer simultaneously possesses high radiation directivity and low-loss features, which illustrates a back-to-front ratio of radiation 53 times higher than that of the pure dielectric dimer and an average radiation efficiency 80% higher than that of the pure metallic dimer. In addition, the unique structures of the hybrid hexamer direct almost decrease 75% of the radiation beamwidth, hence heightening the directivity of the nanoantenna based on a hybrid dimer.
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http://dx.doi.org/10.3390/nano12040710DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8875153PMC
February 2022

Identification and Functional Analysis of the Regulatory Elements in the p Promoter.

Genes (Basel) 2022 01 21;13(2). Epub 2022 Jan 21.

College of Animal Science, Jilin University, Changchun 130062, China.

Functional and expressional research of heat shock protein A6 (HSPA6) suggests that the gene is of great value for neurodegenerative diseases, biosensors, cancer, etc. Based on the important value of pigs in agriculture and biomedicine and to advance knowledge of this little-studied HSPA member, the stress-sensitive sites in porcine (p) were investigated following different stresses. Here, two heat shock elements (HSEs) and a conserved region (CR) were identified in the p promoter by a CRISPR/Cas9-mediated precise gene editing strategy. Gene expression data showed that sequence disruption of these regions could significantly reduce the expression of p under heat stress. Stimulation studies indicated that these regions responded not only to heat stress but also to copper sulfate, MG132, and curcumin. Further mechanism studies showed that downregulated p could significantly affect some important members of the HSP family that are involved in HSP40, HSP70, and HSP90. Overall, our results provide a new approach for investigating gene expression and regulation that may contribute to gene regulatory mechanisms, drug target selection, and breeding stock selection.
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http://dx.doi.org/10.3390/genes13020189DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8872561PMC
January 2022

Tuina combined with physical therapy for spasticity of poststroke: A protocol for systematic review and meta-analysis.

Medicine (Baltimore) 2022 Feb;101(6):e28780

Department of Tuina, Traditional Chinese Medicine Hospital of Jilin Province, China.

Background: Limb spasms are a common complication of stroke. It not only affects the quality of life of stroke survivors, but also brings an economic burden. Tuina combined with physical therapy is widely used in the rehabilitation of poststroke spasticity. However, there is no supporting evidence for its efficacy and safety. This study aimed to evaluate the effectiveness and safety of Tuinas combined with physical therapy in the treatment of spasticity after stroke.

Methods: Literature will be collected from the following databases: China Biology Medicine (CBM), Wanfang Database, China National Knowledge Infrastructure (CNKI), Chinese Scientific Journal Database (VIP), PubMed, Embase, Cochrane Library, and Web of Science; We will include randomized controlled trials of Tuina combined with physical therapy for poststroke spasticity range from the establishment to May 1, 2021. There were no limitations to the publication time, and the language was limited to Chinese and English. The primary outcome was evaluated using the Modified Ashworth scale, and the secondary outcomes were the simplified Fugl-Meyer Assessment scale, Modified Barthel Index, Functional Independence Measurement (FIM), and Visual Analog Scale. RevMan V.5.4.1 software was used for the meta-analysis. The Cochrane Intervention System Evaluation Manual analyzes the risk of bias, and the recommended grading assessment, development and evaluation are used to assess the quality of evidence.

Ethics And Dissemination: This study will be based on published systematic review studies, no ethical approval is required and the results of the study will be published in a peer-reviewed scientific journal.

Systematic Review Registration: INPLASY2021110064.
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http://dx.doi.org/10.1097/MD.0000000000028780DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8830876PMC
February 2022

Gallic Acid Alleviates Gut Dysfunction and Boosts Immune and Antioxidant Activities in Puppies Under Environmental Stress Based on Microbiome-Metabolomics Analysis.

Front Immunol 2021 14;12:813890. Epub 2022 Jan 14.

Guangdong Laboratory for Lingnan Modern Agriculture, Guangdong Provincial Key Laboratory of Animal Nutrition Control, National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou, China.

Early-life exposure to environmental stress disrupts the gut barrier and leads to inflammatory responses and changes in gut microbiota composition. Gallic acid (GA), a natural plant polyphenol, has received significant interest for its antioxidant, anti-inflammatory, and antimicrobial properties that support the maintenance of intestinal health. To assess whether dietary supplementation of GA alleviates environmental stress, a total of 19 puppies were randomly allocated to the following three dietary treatments for 2 weeks: 1) basal diet (control (CON)); 2) basal diet + transportation (TS); and 3) basal diet with the addition of 500 mg/kg of GA + transportation (TS+GA). After a 1-week supplementation period, puppies in the TS and TS+GA groups were transported from a stressful environment to another livable location, and puppies in the CON group were then left in the stressful environment. Results indicated that GA markedly reduced the diarrhea rate in puppies throughout the trial period and caused a moderate decline of serum cortisol and HSP-70 levels after transportation. Also, GA alleviated the oxidative stress and inflammatory response caused by multiple environmental stressors. Meanwhile, puppies fed GA had a higher abundance of fecal Firmicutes and and lower Proteobacteria, , and after transportation. As a result, the TS+GA group had the highest total short-chain fatty acids and acetic acid. Also, the fecal and serum metabolomics analyses revealed that GA markedly reversed the abnormalities of amino acid metabolism, lipid metabolism, carbohydrate metabolism, and nucleotide metabolism caused by stresses. Finally, Spearman's correlation analysis was carried out to explore the comprehensive microbiota and metabolite relationships. Overall, dietary supplementation of GA alleviates oxidative stress and inflammatory response in stressed puppies by causing beneficial shifts on gut microbiota and metabolites that may support gut and host health.
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http://dx.doi.org/10.3389/fimmu.2021.813890DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8795593PMC
March 2022

Synergistic Interaction between Hyperuricemia and Abdominal Obesity as a Risk Factor for Metabolic Syndrome Components in Korean Population.

Diabetes Metab J 2022 Jan 20. Epub 2022 Jan 20.

Division of Endocrinology and Metabolism, Department of Internal Medicine, Pusan National University Yangsan Hospital, Pusan National University School of Medicine, Yangsan, Korea.

Background: The present study investigated the role of synergistic interaction between hyperuricemia and abdominal obesity as a risk factor for the components of metabolic syndrome.

Methods: We performed a cross-sectional study using the data of 16,094 individuals from the seventh Korean National Health and Nutrition Examination Survey (2016 to 2018). The adjusted odds ratios of metabolic syndrome and its components were analyzed by multivariate logistic regression analysis. The presence of synergistic interaction between hyperuricemia and abdominal obesity was evaluated by calculating the additive scales-the relative excess risk due to interaction, attributable proportion due to interaction, and synergy index (SI).

Results: There was a synergistic interaction between hyperuricemia and abdominal obesity in hypertriglyceridemia (men: SI, 1.39; 95% confidence interval [CI], 1.01 to 1.98; women: SI, 1.61; 95% CI, 1.02 to 2.69), and low high-density lipoprotein cholesterol (HDL-C) (men: SI, 2.03; 95% CI, 1.41 to 2.91; women: SI, 1.70; 95% CI, 1.05 to 2.95). There was no significant synergistic interaction between hyperuricemia and abdominal obesity for the risk of high blood pressure (men: SI, 1.22; 95% CI, 0.85 to 1.77; women: SI, 1.53; 95% CI, 0.79 to 2.97), and hyperglycemia (men: SI, 1.03; 95% CI, 0.72 to 1.47; women: SI, 1.39; 95% CI, 0.75 to 2.57).

Conclusion: Hyperuricemia and abdominal obesity synergistically increased the risk of hypertriglyceridemia and low HDL-C in both sexes.
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http://dx.doi.org/10.4093/dmj.2021.0166DOI Listing
January 2022

Design and synthesis of dual inhibitors targeting snail and histone deacetylase for the treatment of solid tumour cancer.

Eur J Med Chem 2022 Feb 30;229:114082. Epub 2021 Dec 30.

School of Science, China Pharmaceutical University, 639 Longmian Avenue, Nanjing, 211198, PR China. Electronic address:

Snail and histone deacetylases (HDACs) have an important impact on cancer treatment, especially for their synergy. Therefore, the development of inhibitors targeting both Snail and HDAC might be a promising strategy for the treatment of cancers. In this work, we synthesized a series of Snail/HDAC dual inhibitors. Compound 9n displayed the most potent inhibitory activity against HDAC1 with an IC of 0.405 μM, potent inhibition against Snail with a K of 0.180 μM, and antiproliferative activity in HCT-116 cell lines with an IC of 0.0751 μM. Compound 9n showed a good inhibitory effect on NCI-H522 (GI = 0.0488 μM), MDA-MB-435 (GI = 0.0361 μM), and MCF7 (GI = 0.0518 μM). Docking studies showed that compound 9n can be well docked into the active binding sites of Snail and HDAC. Further studies showed that compound 9n increased histone H4 acetylation in HCT-116 cells and decreased the expression of Snail protein to induce cell apoptosis. These findings highlight the potential for the development of Snail/HDAC dual inhibitors as anti-solid tumour cancer drugs.
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http://dx.doi.org/10.1016/j.ejmech.2021.114082DOI Listing
February 2022

Electrochemical X-ray Diffraction and Raman Spectroscopic Studies Revealing the Alkali-Metal Ion Intercalation Mechanism in Prussian Blue Analogues.

J Phys Chem Lett 2022 Jan 7;13(2):479-485. Epub 2022 Jan 7.

State Key Laboratory of Physical Chemistry of Solid Surfaces, Collaborative Innovation Center of Chemistry for Energy Materials (i-ChEM), Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.

Prussian blue analogues are promising cathode material candidates for aqueous rechargeable metal-ion batteries. Although great efforts have been made on developing materials, there are still rare reports on optimizing cell performance from mechanistic understanding and studies. Here we unveil the alkali-metal-ion intercalation mechanism in Berlin green with a home-built spectroelectrochemical cell for X-ray Diffraction (XRD) and Raman spectroscopies, which allows us to obtain the correlated local structure, crystal structure, redox activity, and potential profiles during the charging and discharging processes. We found that the intercalation of Na follows a solid solution mechanism leading to a high capacity, and the intercalation of K follows a two-phase transition mechanism showing a high voltage. With this understanding, we propose a new strategy using a Na/K hybrid cation electrolyte to realize both high voltage and energy density. This study offers a unique insight for improving the cell performance from the understanding of the reaction mechanism.
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http://dx.doi.org/10.1021/acs.jpclett.1c03918DOI Listing
January 2022

Real-World Survival Outcomes Based on Mutation Status in Chinese Patients With Lung Adenocarcinoma After Complete Resection: Results From the ICAN Study.

JTO Clin Res Rep 2022 Jan 25;3(1):100257. Epub 2021 Nov 25.

Guangdong Lung Cancer Institute, Guangdong Provincial People's Hospital & Guangdong Academy of Medical Sciences, Guangzhou, People's Republic of China.

Introduction: The adjuvant treatment of patients with resected lung adenocarcinoma (LUAD) remains unstandardized. We analyzed the survival outcomes of these patients based on mutation status and adjuvant chemotherapy treatment.

Methods: This noninterventional real-world study (ICAN) enrolled Chinese patients with resected stages I to III LUAD from April 8, 2010, to December 31, 2010. Tumor mutation status and 3-year disease-free survival (DFS) were determined. The extension phase provided long-term follow-up with overall survival (OS) as the primary end point. Secondary end points included DFS and prognostic factors of survival. Survival outcomes based on adjuvant chemotherapy treatment, mutation status, and postoperative stage were analyzed post hoc.

Results: Among 568 patients in the ICAN cohort, 472 continued to the extension phase and remained eligible. The 3-year DFS rate was 58.8%. In the extension cohort, 260 patients (55.1%) had -mutant disease and 207 (43.9%) received adjuvant chemotherapy. At a median follow-up of 109.0 (95% confidence interval [CI]: 106.6-111.4) months, median OS and DFS were 103.3 (95% CI: 101.7-104.9) and 67.4 (95% CI: 49.7-85.2) months, respectively. The 5-year OS and DFS rates were 68.9% (95% CI: 64.3-73.6) and 52.9% (95% CI: 48.2-57.7), respectively. wild-type disease was a significant independent predictor of worse OS (HR = 1.24, 95% CI: 1.07-1.44, = 0.004) based on the Cox regression analysis of common factors. Post hoc subgroup analysis revealed that survival outcomes were not significantly different with adjuvant chemotherapy regardless of mutation status across all postoperative stages.

Conclusions: mutations are common in operable LUAD, and recurrence and mortality after resection were considerable. Adjuvant chemotherapy did not improve survival outcomes, regardless of mutation status and postoperative stage.
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http://dx.doi.org/10.1016/j.jtocrr.2021.100257DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8683612PMC
January 2022

Deoxyribonuclease 1-like 3 Inhibits Hepatocellular Carcinoma Progression by Inducing Apoptosis and Reprogramming Glucose Metabolism.

Int J Biol Sci 2022 1;18(1):82-95. Epub 2022 Jan 1.

Department of Hepatobiliary Surgery, Tianjin Medical University General Hospital , Tianjin, 300052, P.R. China.

HCC has remained one of the challenging cancers to treat, owing to the paucity of drugs targeting the critical survival pathways. Considering the cancer cells are deficient in DNase activity, the increase of an autonomous apoptisis endonuclease should be a reasonable choice for cancer treatment. In this study, we investigated whether DNASE1L3, an endonuclease implicated in apoptosis, could inhibit the progress of HCC. We found DNASE1L3 was down-regulated in HCC tissues, whereas its high expression was positively associated with the favorable prognosis of patients with HCC. Besides, serum DNASE1L3 levels were lower in HCC patients than in healthy individuals. Functionally, we found that DNASE1L3 inhibited the proliferation of tumor cells by inducing G0/G1 cell cycle arrest and cell apoptosis . Additionally, DNASE1L3 overexpression suppressed tumor growth . Furthermore, we found that DNASE1L3 overexpression weakened glycolysis in HCC cells and tissues via inactivating the rate-limiting enzymes involved in PTPN2-HK2 and CEBPβ-p53-PFK1 pathways. Finally, we identified the HBx to inhibit DNASE1L3 expression by up-regulating the expression of ZNF384. Collectively, our findings demonstrated that DNASE1L3 could inhibit the HCC progression through inducing cell apoptosis and weakening glycolysis. We believe DNASE1L3 could be considered as a promising prognostic biomarker and therapeutic target for HCC.
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http://dx.doi.org/10.7150/ijbs.57919DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8692146PMC
March 2022

Exome-Wide Analyses in Paroxysmal Kinesigenic Dyskinesia Confirm TMEM151A as a Novel Causative Gene.

Mov Disord 2022 03 31;37(3):641-643. Epub 2021 Dec 31.

Department of Neurology and Institute of Neurology of First Affiliated Hospital, Institute of Neuroscience, and Fujian Key Laboratory of Molecular Neurology, Fujian Medical University, Fuzhou, China.

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http://dx.doi.org/10.1002/mds.28904DOI Listing
March 2022
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