Publications by authors named "Kang Lu"

189 Publications

powder alleviates the hippocampal neuron damage in chronic unpredictable mild stress-induced depression model rats in hippocampus via connexin 43Cx43/glucocorticoid receptor/brain-derived neurotrophic factor signaling pathway.

Bioengineered 2022 01;13(1):383-394

Department of Orthopaedics, Zhuhai People's Hospital, Zhuhai City, China.

Powder (XYP) has been widely applied in China to treat stress-related illnesses, such as migraine, depression, Parkinson's disease, insomnia, and hypertension. Herein, this study aims to explore the effect of XYP on chronic unpredictable mild stress (CUMS)-induced depression and its underlying mechanisms. CUMS-induced depression rat models were established, they were subsequently randomly divided and treated with various conditions. Results of this study indicated that supplementation of XYP observably abolished CUMS-induced hippocampal damage and serum corticosterone (CORT) elevation. In mechanism, we discovered that CUMS induction could cause a prominent downregulation in glucocorticoid receptor (GR), phosphorylated-GR (p-GR), connexin 43 (Cx43), and brain-derived neurotrophic factor (BDNF), a remarkable upregulation in c-Src. While the introduction of XYP could reverse the changes in all of these indicators mediated by CUMS. Furthermore, we proved that Cx43 could interact with GR, and the protective effect of XYP on hippocampal neurons is realized by up-regulating GR. Summarized, this study indicated that XYP could ameliorate hippocampal neuron damage in CUMS-induced depression model rats through acting on Cx43/GR/BDNF axis.
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http://dx.doi.org/10.1080/21655979.2021.2005744DOI Listing
January 2022

Multi-residue analytical method development and risk assessment of 56 pesticides and their metabolites in tea by chromatography tandem mass spectroscopy.

Food Chem 2022 May 9;375:131819. Epub 2021 Dec 9.

Innovation Center of Pesticide Research, Department of Applied Chemistry, College of Science, China Agricultural University, Beijing 100193, China. Electronic address:

A modified QuEChERS method coupled to chromatography tandem mass spectroscopy was established and used to identify 56 pesticides and 21 metabolites residues in tea samples. The average recoveries for the target compounds ranged from 71% to 109% with RSDs of 1-17%. Pesticides and metabolites residues in 248 tea samples from China were investigated by the developed method, and the dietary intake risk for consumers was estimated. The results showed that 36 pesticides and 14 metabolites were detected with concentrations of 0.0050-7.7 mg/kg. There are unlikely to present a public health concern for Chinese consumers. The acute risk of pesticides would be exaggerated when calculated by the residue level in tea instead of tea infusion. The unauthorized and banned pesticide contributed to the chronic and acute hazard index by 68% and 36%, respectively. The cumulative risk of residual pesticides and their metabolites on human health deserve further attention.
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http://dx.doi.org/10.1016/j.foodchem.2021.131819DOI Listing
May 2022

Simultaneous quantification of eleven short-chain fatty acids by derivatization and solid phase microextraction - Gas chromatography tandem mass spectrometry.

J Chromatogr A 2022 Jan 11;1661:462680. Epub 2021 Nov 11.

Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, P.R. China. Electronic address:

As metabolites of the gut microbiome, short-chain fatty acids (SCFAs) played an important role in the diagnosis of the metabolic diseases. Because of the high polarity, high volatility and complex matrix of biological samples, the highly sensitive, selective and accurate method to determine SCFAs remains a major challenge. Herein, a new method for simultaneous quantification of eleven SCFAs by derivatization combined with solid phase microextraction (SPME) and gas chromatography tandem mass spectrometry (GC-MS/MS) was developed. Isobutyl chloroformate coupled with isobutanol was used as the reaction reagent to derivatize SCFAs. The method validation data showed a satisfactory linearity with the linear regression coefficients (R) ranging from 0.9964 to 0.9996. The limit of detection (LOD) of all SCFAs ranges from 0.01 ng·mL to 0.72 ng·mL and the limit of quantification (LOQ) ranges from 0.04 ng·mL to 2.41 ng·mL. The intra-day and inter-day precision (RSDs) ranged from 0.65% to 8.92% and 1.62% to 15.61%, respectively. The recovery ranged from 88.10% to 108.71%. Finally, the developed method was successfully used to determine SCFAs in mice fecal sample, and ten of the SCFAs were found in feces of mice, including formic acid.
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http://dx.doi.org/10.1016/j.chroma.2021.462680DOI Listing
January 2022

Corrigendum to: Genomic Diversity of Severe Acute Respiratory Syndrome-Coronavirus 2 in Patients With Coronavirus Disease 2019.

Clin Infect Dis 2021 Dec;73(12):2374

Key Laboratory of Genomic and Precision Medicine, Beijing Institute of Genomics, Chinese Academy of Sciences, and China National Center for Bioinformation, Beijing, China.

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http://dx.doi.org/10.1093/cid/ciab900DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8689987PMC
December 2021

Nanoselenium Enhanced Wheat Resistance to Aphids by Regulating Biosynthesis of DIMBOA and Volatile Components.

J Agric Food Chem 2021 Dec 17;69(47):14103-14114. Epub 2021 Nov 17.

Innovation Center of Pesticide Research, Department of Applied Chemistry, College of Science, China Agricultural University, China Yuanmingyuan West Road 2, Beijing 100193, P. R. China.

The mechanism of nanoselenium (nano-Se) improving the resistance induced by plant components to aphids is unclear. In this study, foliar sprayed nano-Se (5.0 mg/L) could significantly reduce the number (36%) compared with that in the control. Foliar application of nano-Se enhanced the antioxidant capacity by reducing malondialdehyde (MDA) and increasing GSH-Px, CAT, GSH, Pro, and VE concentrations in wheat seedlings. The phenylpropane pathway was activated by nano-Se biofortification, which increased apigenin and caffeic acid concentrations. The high-level expression of the related genes (1, 3, 4, 2, and ) induced the promotion of melatonin (88.6%) and 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one (DIMBOA) (64.3%). Different ratios of the secondary metabolites to nano-Se were taken to examine the effects on resistance of wheat to . The results revealed that the combination of nano-Se and melatonin could achieve the best overall performance by reducing the number by 52.2%. The study suggests that the coordinated applications of nano-Se and melatonin could more effectively improve the wheat resistance to aphids via the promotion of volatile organic compound synthesis and modulation in phenylpropane and indole metabolism pathways.
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http://dx.doi.org/10.1021/acs.jafc.1c05617DOI Listing
December 2021

Effects of aging on the histology and biochemistry of rat tendon healing.

BMC Musculoskelet Disord 2021 Nov 15;22(1):949. Epub 2021 Nov 15.

Department of Sports Medicine Center, Southwest Hospital, Army Medical University, Chongqing, 400038, China.

Introduction: Tendon diseases and injuries are a serious problem for the aged population, often leading to pain, disability and a significant decline in quality of life. The purpose of this study was to determine the influence of aging on biochemistry and histology during tendon healing and to provide a new strategy for improving tendon healing.

Method: A total of 24 Sprague-Dawley rats were equally divided into a young and an aged group. A rat patellar tendon defect model was used in this study. Tendon samples were collected at weeks 2 and 4, and hematoxylin-eosin, alcian blue and immunofluorescence staining were performed for histological analysis. Meanwhile, reverse transcription-polymerase chain reaction (RT-PCR) and western blot were performed to evaluate the biochemical changes.

Results: The histological scores in aged rats were significantly lower than those in young rats. At the protein level, collagen synthesis-related markers Col-3, Matrix metalloproteinase-1 and Metallopeptidase Inhibitor 1(TIMP-1) were decreased at week 4 in aged rats compared with those of young rats. Though there was a decrease in the expression of the chondrogenic marker aggrecan at the protein level in aged tendon, the Micro-CT results from weeks 4 samples showed no significant difference(p>0.05) on the ectopic ossification between groups. Moreover, we found more adipocytes accumulated in the aged tendon defect with the Oil Red O staining and at the gene and protein levels the markers related to adipogenic differentiation.

Conclusions: Our findings indicate that tendon healing is impaired in aged rats and is characterized by a significantly lower histological score, decreased collagen synthesis and more adipocyte accumulation in patellar tendon after repair.
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http://dx.doi.org/10.1186/s12891-021-04838-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8594129PMC
November 2021

Genomic Epidemiology of SARS-CoV-2 in Pakistan.

Genomics Proteomics Bioinformatics 2021 Oct 22. Epub 2021 Oct 22.

China National Center for Bioinformation, Beijing 100101, China; National Genomics Data Center, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China; CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address:

COVID-19 has swept globally and Pakistan is no exception. To investigate the initial introductions and transmissions of the SARS-CoV-2 in Pakistan, we performed the largest genomic epidemiology study of COVID-19 in Pakistan and generated 150 complete SARS-CoV-2 genome sequences from samples collected before June 2, 2020. We identified a total of 347 mutated positions, 31 of which were over-represented in Pakistan. Meanwhile, we found over 1000 intra-host single-nucleotide variants (iSNVs). Several of them occurred concurrently, indicating possible interactions among them or coevolution. Some of the high-frequency iSNVs in Pakistan were not observed in the global population, suggesting strong purifying selections. The genomic epidemiology revealed five distinctive spreading clusters. The largest cluster consisted of 74 viruses which were derived from different geographic locations of Pakistan and formed a deep hierarchical structure, indicating an extensive and persistent nation-wide transmission of the virus that was probably attributed to a signature mutation (G8371T in ORF1ab) of this cluster. Furthermore, 28 putative international introductions were identified, several of which are consistent with the epidemiological investigations. In all, this study has inferred the possible pathways of introduction and transmissions of SARS-CoV-2 in Pakistan, which could aid ongoing and future viral surveillance and COVID-19 control.
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http://dx.doi.org/10.1016/j.gpb.2021.08.007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8546014PMC
October 2021

Cold-chain food contamination as the possible origin of COVID-19 resurgence in Beijing.

Natl Sci Rev 2020 Dec 23;7(12):1861-1864. Epub 2020 Oct 23.

NHC Key Laboratory of Systems Biology of Pathogens and Christophe Mérieux Laboratory, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, China.

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http://dx.doi.org/10.1093/nsr/nwaa264DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7665658PMC
December 2020

Adipogenic differentiation was inhibited by downregulation of PPARγ signaling pathway in aging tendon stem/progenitor cells.

J Orthop Surg Res 2021 Oct 18;16(1):614. Epub 2021 Oct 18.

Department of Sports Medicine Center, Southwest Hospital, Army Medical University, Chongqing, 400038, China.

Background: Tendon stem/progenitor cells (TSPCs) play a vital role in tendon repair and regeneration. Previously we found more adipocytes accumulated in the patellar tendon injury sites in aging rats compared with the young ones, of which the mechanism is still unknown. Here, we want to identify whether erroneous differentiation of TSPCs by aging accounts for the adipocyte accumulation.

Methods: TSPCs from young and aging rats were isolated and propagated. Both young and aging TSPCs were induced to differentiate into adipocytes, and Oil red O staining, quantitative real-time polymerase chain reaction (qRT-PCR), western-blot and immunofluorescent staining were used to evaluate the capability of TSPCs. RNA sequencing was utilized to screen out different genes and signaling pathways related to adipogenesis between young and aging TSPCs.

Results: The Oil red O staining showed there were more adipocytes formed in young TSPCs. Besides, adipogenic markers perilipin, peroxisome proliferator-activated receptor γ (PPARγ), CCAAT/enhancer-binding proteins alpha (C/EBPα) and Fatty acid-binding protein 4 (FABP4) were elevated both at gene and protein level. PPARγ signaling pathway was selected as our target via RNA sequencing. After adding the signaling activators, Rosiglitazone maleate (RM), inhibited adipogenesis of aging TSCs was reversed.

Conclusions: In conclusion, aging inhibited adipogenesis of TSPCs by down-regulating PPARγ signaling. It is not likely that the adipocyte accumulation in aging tendon during repair was due to the aging of TSPCs. This may provide new targets for curing aging tendon injuries or tendinopathies.
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http://dx.doi.org/10.1186/s13018-021-02720-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8522149PMC
October 2021

Nanoselenium transformation and inhibition of cadmium accumulation by regulating the lignin biosynthetic pathway and plant hormone signal transduction in pepper plants.

J Nanobiotechnology 2021 Oct 12;19(1):316. Epub 2021 Oct 12.

Innovation Center of Pesticide Research, Department of Applied Chemistry, College of Science, China Agricultural University, Beijing, 100193, China.

Selenium (Se) can promote the growth and resistance of agricultural crops as fertilizers, while the role of nano-selenium (nano-Se) against Cd remains unclear in pepper plants (Capsicum annuum L.). Biofortification with nano-Se observably restored Cd stress by decreasing the level of Cd in plant tissues and boosting the accumulation in biomass. The Se compounds transformed by nano-Se were primarily in the form of SeMet and MeSeCys in pepper tissues. Differential metabolites and the genes of plant signal transduction and lignin biosynthesis were measured by employing transcriptomics and determining target metabolites. The number of lignin-related genes (PAL, CAD, 4CL, and COMT) and contents of metabolites (sinapyl alcohol, phenylalanine, p-coumaryl alcohol, caffeyl alcohol, and coniferaldehyde) were remarkably enhanced by treatment with Cd1Se0.2, thus, maintaining the integrity of cell walls in the roots. It also enhanced signal transduction by plant hormones and responsive resistance by inducing the biosynthesis of genes (BZR1, LOX3, and NCDE1) and metabolites (brassinolide, abscisic acid, and jasmonic acid) in the roots and leaves. In general, this study can enable a better understanding of the protective mechanism of nano-Se in improving the capacity of plants to resist environmental stress.
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http://dx.doi.org/10.1186/s12951-021-01061-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8507250PMC
October 2021

Pesticide Residue Behavior and Risk Assessment in Celery after Se Nanoparticles Application.

Foods 2021 Aug 25;10(9). Epub 2021 Aug 25.

Key Laboratory of Agro-Products Quality and Safety of Xinjiang, Institute of Agricultural Quality Standards and Testing Technology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China.

This study investigates pesticide levels in celery, and compares their degradation, dissipation, distribution, and dietary risk after spraying with selenium (Se) nanoparticles. Abamectin, imidacloprid, acetamiprid, thiamethoxam, and lambda-cyhalothrin were sprayed at 1.6, 6.8, 2.0, 1.0, and 0.7 g a.i. ha followed by a 2 g·ha Se nanoparticle application during the growing period. Thiamethoxam, abamectin, imidacloprid, lambda-cyhalothrin, and acetamiprid in celery degraded following a first order kinetic model after 2 g·ha Se nanoparticles application. With the exception of acetamiprid, the half-lives of thiamethoxam, abamectin, imidacloprid, and lambda-cyhalothrin were reduced from 2.4, 0.5, 1.2, 4.2 days without Se nanoparticles application to 1.4, 0.2, 0.9, 3.7 days with the addition of Se nanoparticles (2 g·ha), respectively. The chronic dietary exposure risk probability (RQc) and the acute dietary exposure risk probability (RQa) of celery after Se nanoparticles application were within acceptable limits for consumption except for abamectin.
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http://dx.doi.org/10.3390/foods10091987DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8470415PMC
August 2021

Dynamics of the Upper Respiratory Tract Microbiota and Its Association with Mortality in COVID-19.

Am J Respir Crit Care Med 2021 12;204(12):1379-1390

Beijing Institute of Genomics, Chinese Academy of Sciences, and China National Center for Bioinformation, Beijing, China.

Alteration of human respiratory microbiota had been observed in coronavirus disease (COVID-19). How the microbiota is associated with the prognosis in COVID-19 is unclear. To characterize the feature and dynamics of the respiratory microbiota and its associations with clinical features in patients with COVID-19. We conducted metatranscriptome sequencing on 588 longitudinal oropharyngeal swab specimens collected from 192 patients with COVID-19 (including 39 deceased patients) and 95 healthy controls from the same geographic area. Meanwhile, the concentration of 27 cytokines and chemokines in plasma was measured for patients with COVID-19. The upper respiratory tract (URT) microbiota in patients with COVID-19 differed from that in healthy controls, whereas deceased patients possessed a more distinct microbiota, both on admission and before discharge/death. The alteration of URT microbiota showed a significant correlation with the concentration of proinflammatory cytokines and mortality. Specifically, -dominated microbiota was enriched in recovered patients, and showed high temporal stability and resistance against pathogens. In contrast, the microbiota in deceased patients was more susceptible to secondary infections and became more deviated from the norm after admission. Moreover, the abundance of on admission was significantly correlated with prognosis in nonsevere patients (lower vs. higher abundance, odds ratio, 7.80; 95% CI, 1.70-42.05). URT microbiota dysbiosis is a remarkable manifestation of COVID-19; its association with mortality suggests it may reflect the interplay between pathogens, symbionts, and the host immune status. Whether URT microbiota could be used as a biomarker for diagnosis and prognosis of respiratory diseases merits further investigation.
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http://dx.doi.org/10.1164/rccm.202103-0814OCDOI Listing
December 2021

Rapid greening response of China's 2020 spring vegetation to COVID-19 restrictions: Implications for climate change.

Sci Adv 2021 Aug 25;7(35). Epub 2021 Aug 25.

State Key Laboratory of Resources and Environmental Information System, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China.

The 2019 novel coronavirus pandemic (COVID-19) negatively affected global public health and socioeconomic development. Lockdowns and travel restrictions to contain COVID-19 resulted in reduced human activity and decreased anthropogenic emissions. However, the secondary effects of these restrictions on the biophysical environment are uncertain. Using remotely sensed big data, we investigated how lockdowns and traffic restrictions affected China's spring vegetation in 2020. Our analyses show that travel decreased by 58% in the first 18 days following implementation of the restrictions across China. Subsequently, atmospheric optical clarity increased and radiation levels on the vegetation canopy were augmented. Furthermore, the spring of 2020 arrived 8.4 days earlier and vegetation 17.45% greener compared to 2015-2019. Reduced human activity resulting from COVID-19 restrictions contributed to a brighter, earlier, and greener 2020 spring season in China. This study shows that short-term changes in human activity can have a relatively rapid ecological impact at the regional scale.
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http://dx.doi.org/10.1126/sciadv.abe8044DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8386938PMC
August 2021

Structural Engineering of Hollow Microflower-like [email protected] Hybrids as Versatile Electrochemical Sensing Platform for Highly Sensitive Hydrogen Peroxide and Hydrazine Detection.

ACS Appl Mater Interfaces 2021 Sep 20;13(34):40942-40952. Epub 2021 Aug 20.

School of Electronic Information Engineering, Yangtze Normal University, Chongqing 408100, China.

Designing metal sulfides with unique configurations and exploring their electrochemical activities for hydrogen peroxide (HO) and hydrazine (NH) is challenging and desirable for various fields. Herein, hollow microflower-like [email protected] hybrids were successfully assembled and further exploited as a versatile electrochemical sensing platform for HO reduction and NH oxidation, of which the elaborate strategies make the perfect formation of hollow architecture, providing considerable electrocatalytic sites and fast charge transfer rate, while the appropriate introduction polydopamine-derived carbon skeleton facilitates the electronic conductivity and boosts structural robustness, thus generating wide linear range (0.05-14 and 0.01-10 mM), low detection limit (0.22 μM and 0.07 μM), and a rather low overpotential (-0.15 and -0.05 V) toward HO and NH, as well as good selectivity, excellent reproducibility, and admirable long-term stability. It should be highlighted that the operating potentials can compare favorably with those of some reported HO and NH sensors based on noble metals. In addition, good recoveries and acceptable relative standard deviations (RSDs) attained in serum and water samples fully verify the accuracy and anti-interference capability of our proposed sensor systems. These results not only elucidate an effective structural nanoengineering strategy for electroanalytical science but also advance the rational utilization of HO and NH in practicability.
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http://dx.doi.org/10.1021/acsami.1c11747DOI Listing
September 2021

Intranodal Sirolimus Induces Regulatory T Cells in Human Hepatic Lymph Nodes via Interleukin 10 Signaling.

Liver Transpl 2021 11 31;27(11):1669-1672. Epub 2021 Jul 31.

Department of Biology, University of North Florida, Jacksonville, FL.

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http://dx.doi.org/10.1002/lt.26214DOI Listing
November 2021

Acupuncture May Decrease the Incidence of Post-stroke Dementia: A Taiwan Nationwide Retrospective Cohort Study.

Front Neurol 2021 21;12:657048. Epub 2021 May 21.

Department of Neurosurgery, E-Da Hospital, Kaohsiung, Taiwan.

Post-stroke dementia may affect up to one-third of stroke survivors. Acupuncture as a complementary treatment for stroke has been shown to be beneficial for subsequent post-stroke rehabilitation. The purpose of this retrospective cohort study was to investigate the potential effect of acupuncture to protect stroke patients from dementia. We included 9,547 patients receiving ambulatory or hospital care for stroke and 9.547 non-stroke patients; patients were matched for sex, age, and Charlson Comorbidity Index. Each individual was traced for the subsequent development of dementia. Two thousand four hundred and forty-nine stroke patients received acupuncture treatment and 7,098 residue stroke patients without acupuncture treatment served as control groups. This is a 3-year follow-up cohorts study: the incidence and adjusted hazard ratios (HRs) with 95% confidence intervals (CIs) of post- stroke dementia in the Cox proportional hazard regression. During the 3-year follow-up, 1,403 patients with stroke (14.70%) and 427 patients without stroke (4.47%) developed dementia. The adjusted HRs of development of dementia among stroke patients were 3.64-times (range, 3.27-4.06), and the incidence of dementia was higher in male. Stroke patients receiving acupuncture treatment had a lower probability of dementia than those without acupuncture during the follow-up period, the adjusted HRs was 0.49 (95% CI, 0.42-0.58; < 0.001). The association between stroke and dementia existed in both sexes, more prominent in male. Patients with stroke receiving acupuncture treatments showed decreased risk of dementia. Care must be taken evaluating these results because this study was limited to lack of information regarding lifestyles, stroke severities, and acupuncture methods that were used in treatments.
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http://dx.doi.org/10.3389/fneur.2021.657048DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8176024PMC
May 2021

Rapid determination of folic acid and riboflavin in urine by polypyrrole magnetic solid-phase extractant combined ultra-performance liquid chromatography.

J Chromatogr A 2021 Jul 27;1648:462192. Epub 2021 Apr 27.

Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China. Electronic address:

Determination of folic acid and riboflavin in biological samples is difficult due to their high polarity, low concentration, chemical instability, and complex matrix. In this study, the polypyrrole-coated magnetic nanocomposite ([email protected]) was synthesized innovatively with the assistance of hexadecyltrimethylammonium bromide. To evaluate the adsorption mechanism and the feasibility of synthesized [email protected] as an adsorbent, the adsorption capacities, kinetics and thermodynamics of folic acid and riboflavin were investigated systemically. Furthermore, in light of the chemical instability of folic acid and riboflavin a method for rapid extraction and detection of them from human urine within 10 min was developed successfully by combining magnetic solid phase extraction with ultra-performance liquid chromatography (MSPE/UPLC). The adsorption parameters including sorbent amount, pH value, extraction time, desorption solvent and desorption time were studied. Under optimum conditions, the performance of the established determination method was validated with the linearly dependent coefficients (>0.9995), the limits of detection (0.02-0.05 µg/mL), the limits of quantification (0.07-0.18 µg/mL), and the recoveries (92.2-105.1%, with relative standard deviation < 3.3%). The rapid extraction and detection of folic acid and riboflavin from real urine samples were achieved subsequently. The present study suggests that the developed method exhibits a promising application in the analysis of free folic acid and riboflavin in human urine samples, which can provide a reference for the clinical drug monitoring and treatment.
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http://dx.doi.org/10.1016/j.chroma.2021.462192DOI Listing
July 2021

Corrigendum to "Bionic Silk Fibroin Film Promotes Tenogenic Differentiation of Tendon Stem/Progenitor Cells by Activating Focal Adhesion Kinase".

Stem Cells Int 2021 2;2021:6317062. Epub 2021 Mar 2.

Department of Orthopedics/Sports Medicine Center, State Key Laboratory of Trauma, Burn and Combined Injury, Southwest Hospital, Army Medical University (Third Military Medical University), Chongqing 400038, China.

[This corrects the article DOI: 10.1155/2020/8857380.].
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http://dx.doi.org/10.1155/2021/6317062DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7946452PMC
March 2021

Wilms tumor gent 1 (WT1)-specific adoptive immunotherapy in hematologic diseases.

Int Immunopharmacol 2021 May 28;94:107504. Epub 2021 Feb 28.

Department of Hematology, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan 250021, China; Department of Hematology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan 250021, China; School of Medicine, Shandong University, Jinan, Shandong 250012, China. Electronic address:

As an attractive tumor-associated antigen (TAA), Wilms tumor gene 1 (WT1) is usually overexpressed in malignant hematological diseases. In recent years, WT1-specific adoptive immunotherapy has been the "hot spot" for tumor treatment. The main immunotherapeutic techniques associated with WT1 include WT1-specific cytotoxic T lymphocytes (CTLs), vaccine, and T cell receptor (TCR) gene therapy. WT1-based adoptive immunotherapy exhibited promising anti-tumorous effect with tolerable safety. There are still many limitations needed to be improved including the weak immunogenetics of WT1, immune tolerance, and short persistence of the immune response. In this review, we summarized the progress of productive technologies and the clinical or preclinical investigations of WT1-specific immunotherapy in hematological diseases.
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http://dx.doi.org/10.1016/j.intimp.2021.107504DOI Listing
May 2021

Sleep deprivation did not enhance the success rate of chloral hydrate sedation for non-invasive procedural sedation in pediatric patients.

PLoS One 2021 12;16(1):e0245338. Epub 2021 Jan 12.

Department of Anesthesiology, The Affiliated Hospital, School of Medicine, UESTC Chengdu Women's & Children's Central Hospital, China.

Study Objective: In Asian countries, oral chloral hydrate is the most commonly used sedative for non-invasive procedures. Theoretically, mild sleep deprivation could be considered as one of assisted techniques. However, there is no consensus on sleep deprivation facilitating the sedation during non-painful procedures in children. The aim of our study is to analyze the clinical data of children undergoing non-invasive procedural sedation retrospectively and to evaluate the association between mild sleep deprivation and sedative effects in non-invasive procedures.

Measurements: Consecutive patients undergoing chloral hydrate sedation for non-invasive procedures between December 1, 2019 to June 30, 2020 were included in this study. The propensity score analysis with 1: 1 ratio was used to match the baseline variables between patients with sleep deprivation and non-sleep deprivation. The primary outcome was the failure rate of sedation with the initial dose. The secondary outcomes included the failure rate of sedation after supplementation of chloral hydrate, the incidence of major and minor adverse events, initial and supplemental dose of chloral hydrate, and the length of sedation time.

Main Results: Of the 7789 patients undergoing chloral hydrate sedation, 6352 were treated with sleep deprivation and 1437 with non-sleep deprivation. After propensity score matching, 1437 pairs were produced. The failure rate of sedation with initial chlorate hydrate was not significantly different in two groups (8.6% [123/1437] vs. 10.6% [152/1437], p = 0.08), nor were the failure rates with supplemental chlorate hydrate (0.8% [12/1437] vs. 0.9% [13/1437], p = 1) and the length of sedation time (58 [45, 75] vs. 58 [45, 75] min; p = 0.93).

Conclusions: The current results do not support sleep deprivation have a beneficial effect in reducing the pediatric chloral hydrate sedation failure rate. The routine use of sleep deprivation for pediatric sedation is unnecessary.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0245338PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7802942PMC
June 2021

The prognostic roles of hepatitis B virus antibody in diffuse large B-cell lymphoma patients.

Leuk Lymphoma 2021 06 5;62(6):1335-1343. Epub 2021 Jan 5.

Department of Hematology, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan, China.

Diffuse large B-cell lymphoma (DLBCL) has been correlated with virus infection and immunity status. We retrospectively analyzed the association between HBV antibody and DLBCL development in HBsAg patients. Compared with HBeAb patients, HBeAb patients displayed unique clinical features. HBV antibody-negative patients had better therapeutic efficiency ( < .05). The media progression-free survival (PFS) and overall survival (OS) of HBV antibody-positive group were shorter than the negative group ( < .05). Furthermore, we found positive association between CD21 and HBsAb and their synergistic effect for prognostic predication. Interestingly, the effect of Rituximab in prognostic improvement was more significant in HBV antibody-positive group than negative group. Univariate analysis showed that HBV antibody was independent risk factor for disease prognosis. Altogether, our investigations identified for the first time the close association between HBV antibody and clinical prognosis in DLBCL patients. These findings provide potential biomarker to predict the effect of Rituximab and prognosis in DLBCL patients.
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http://dx.doi.org/10.1080/10428194.2020.1867726DOI Listing
June 2021

Dynamics of severe acute respiratory syndrome coronavirus 2 genome variants in the feces during convalescence.

J Genet Genomics 2020 10 8;47(10):610-617. Epub 2020 Nov 8.

Key Laboratory of Genomic and Precision Medicine, Beijing Institute of Genomics, Chinese Academy of Sciences, China National Center for Bioinformation, Beijing, 101300, China; University of Chinese Academy of Sciences, Beijing, 100049, China; Center for Excellence in Animal Evolution and Genetics, Chinese Academy of Sciences, Kunming, 650223, China. Electronic address:

In response to the current coronavirus disease 2019 (COVID-19) pandemic, it is crucial to understand the origin, transmission, and evolution of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which relies on close surveillance of genomic diversity in clinical samples. Although the mutation at the population level had been extensively investigated, how the mutations evolve at the individual level is largely unknown. Eighteen time-series fecal samples were collected from nine patients with COVID-19 during the convalescent phase. The nucleic acids of SARS-CoV-2 were enriched by the hybrid capture method. First, we demonstrated the outstanding performance of the hybrid capture method in detecting intra-host variants. We identified 229 intra-host variants at 182 sites in 18 fecal samples. Among them, nineteen variants presented frequency changes > 0.3 within 1-5 days, reflecting highly dynamic intra-host viral populations. Moreover, the evolution of the viral genome demonstrated that the virus was probably viable in the gastrointestinal tract during the convalescent period. Meanwhile, we also found that the same mutation showed a distinct pattern of frequency changes in different individuals, indicating a strong random drift. In summary, dramatic changes of the SARS-CoV-2 genome were detected in fecal samples during the convalescent period; whether the viral load in feces is sufficient to establish an infection warranted further investigation.
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http://dx.doi.org/10.1016/j.jgg.2020.10.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7649052PMC
October 2020

Improved ultra-high performance liquid chromatographic method for simultaneous determination of five gout-related metabolites in human serum.

J Sep Sci 2021 Mar 29;44(5):954-962. Epub 2021 Jan 29.

Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, P. R. China.

Creatinine and purines are gout-related metabolites commonly quantified by liquid chromatography coupled with ultraviolet and mass spectrometry. However, the high cost of liquid chromatography coupled with mass spectrometry hindered its extensive use in ordinary hospitals and clinical laboratories. Using the traditional liquid chromatography method, the full separation of these metabolites in complex biological samples is still not achieved. In this study, an improved ultra-high-performance liquid chromatography with ultraviolet spectroscopy method was reported for quantitative determination of five gout-related metabolites (i.e., creatinine, uric acid, hypoxanthine, xanthine, and inosine) in human serum within 10 min. A UHPLC system equipped with a hydrophilic C18 column was used to improve separation, shorten analysis time, and increase analysis throughput. The performance of the method was validated by evaluating linearity (squared correlation coefficient > 0.9991), recovery (92.8-100.0%, with relative standard deviation < 4.7%), accuracy (relative errors < 14.6%), precision (0.2-4.1% for intraday and 2.1-7.3% for interday) and stability (-14.1 to 8.3% in autosampler for 12 h and -13.3 to 2.2% for freeze-thaw cycles). This method was successfully applied to quantify gout-related metabolites in serum samples of healthy controls and gout patients, which was expected to be used in the clinical investigation of gout at different stages.
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http://dx.doi.org/10.1002/jssc.202000974DOI Listing
March 2021

Bionic Silk Fibroin Film Induces Morphological Changes and Differentiation of Tendon Stem/Progenitor Cells.

Appl Bionics Biomech 2020 1;2020:8865841. Epub 2020 Dec 1.

Department of Orthopedics/Sports Medicine Center, State Key Laboratory of Trauma, Burn and Combined Injury, Southwest Hospital, Army Medical University (Third Military Medical University), Chongqing 400038, China.

Purpose: Tendon injuries are common musculoskeletal system disorders, but the ability for tendon regeneration is limited. Silk fibroin (SF) film may be suitable for tendon regeneration due to its excellent biocompatibility and physical properties. This study is aimed at evaluating the application value of bionic SF film in tendon regeneration.

Methods: Tendon stem/progenitor cells (TSPCs) were isolated from rat Achilles tendon and characterized based on their surface marker expression and multilineage differentiation potential. SF films with smooth or bionic microstructure surfaces (5, 10, 15, 20 m) were prepared. The morphology and mechanical properties of natural tendons and SF films were characterized. TSPCs were used as the seed cells, and the cell viability and cell adhesion morphology were analyzed. The tendongenesis-related gene expression of TSPCs was also evaluated using quantitative polymerase chain reaction.

Results: Compared to the native tendon, only the 10, 15, and 20 m SF film groups had comparable maximum loading and ultimate stress, with the exception of the breaking elongation rate. The 10 m SF film group had the highest percentage of oriented cells and the most significant changes in cell morphology. The most significant upregulations in the expression of , , and were also observed in the 10 m SF film group.

Conclusion: SF film with a bionic microstructure can serve as a tissue engineering scaffold and provide biophysical cues for the use of TSPCs to achieve proper cellular adherence arrangement and morphology as well as promote the tenogenic differentiation of TSPCs, making it a valuable customizable biomaterial for future applications in tendon repair.
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http://dx.doi.org/10.1155/2020/8865841DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7725557PMC
December 2020

Hydrogel Electrolytes for Quasi-Solid Zinc-Based Batteries.

Front Chem 2020 4;8:546728. Epub 2020 Nov 4.

Key Laboratory of Structure and Functional Regulation of Hybrid Materials, Anhui University, Ministry of Education, Hefei, China.

On account of high energy density depending on the utilized zinc metal anode of high theoretical capacity and its excellent security due to aqueous electrolytes that usually be locked in polymer hosts referred to as hydrogels, quasi-solid zinc-based batteries have been subjected to more and more interest from researchers. The good water retention and electrolyte load capacity of the hydrogel, contributing to the acquirement of high ionic conductivity and durability of the as-obtained quasi-solid electrolyte, play a significant role on the performance of the devices. Moreover, the chemistry of hydrogels can be tuned to endow quasi-solid electrolytes with additional functions in terms of application scenarios of solid-state batteries. Herein, the frontier disciplines of hydrogel electrolytes for Zn-based batteries were reviewed. The cross-linking process of the polymer networks for hydrogel materials with different functions, such as stretchability, compressibility, and self-healing, were also discussed to analyze the properties of the polymer electrolyte. Based on the merits of the functionalized hydrogel, the further application of hydrogel electrolytes in Zn-based batteries is the focus of this paper. The electrochemical performance and mechanical property of Zn-based batteries with functionalized hydrogel electrolytes under extreme conditions were presented to evaluate the crucial role of the polymer hydrogel electrolyte. Finally, the challenges of hydrogel electrolytes for currently developed Zn-based batteries are highlighted with the hope to boost their commercial application in energy conversion devices.
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http://dx.doi.org/10.3389/fchem.2020.546728DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7672033PMC
November 2020

Pharmacokinetics and Safety of Fosaprepitant Dimeglumine in Healthy Chinese Volunteers: Bioequivalence Study.

Clin Pharmacol Drug Dev 2021 07 5;10(7):748-755. Epub 2020 Dec 5.

Phase I Clinical Research Center, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, Hangzhou, China.

Fosaprepitant dimeglumine (FD) is a precursor of aprepitant. FD can be metabolized into aprepitant. This randomized, single-center, open, 2-cycle, single-dose, crossover bioequivalence study compared the pharmacokinetics (PK) and safety of intravenously FD of test and reference products in healthy volunteers (HVs). HVs were assigned to the test group or reference group randomly and given FD intravenously. The plasma concentration of FD and aprepitant was measured using liquid chromatography-tandem mass spectrometry. PK parameters were ascertained based on a noncompartmental model. Data for 29 HVs were obtained. The geometric mean and 90% confidence intervals of maximum plasma concentration (C ), area under the concentration-time curve from time 0 to time of last measurable plasma concentration (AUC ), and area from the last datum point to time infinity (AUC ) of test and reference groups were 101.69% (95.06%, 108.77%), 103.52% (99.15%, 108.09%), and 105.58% (99.51%, 112.01%), respectively. These 3 parameters were within the acceptance range of 80.0% to 125.00%, and the test product was bioequivalent to the reference product. The coefficient of variation (CV) of C , AUC , and AUC was 15.14%, 9.67%, and 11.89%, respectively. Intravenously administered FD provided by 2 sponsors achieved bioequivalence. FD values from test and reference products were bioequivalent. All adverse events were mild and serious adverse events absent in HVs. This study indicated that FD may provide a safer alternative to aprepitant for chemotherapy-induced nausea and vomiting.
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http://dx.doi.org/10.1002/cpdd.892DOI Listing
July 2021

Bionic Silk Fibroin Film Promotes Tenogenic Differentiation of Tendon Stem/Progenitor Cells by Activating Focal Adhesion Kinase.

Stem Cells Int 2020 4;2020:8857380. Epub 2020 Nov 4.

Department of Orthopedics/Sports Medicine Center, State Key Laboratory of Trauma, Burn and Combined Injury, Southwest Hospital, Army Medical University (Third Military Medical University), Chongqing 400038, China.

Background: Tendon injuries are common musculoskeletal disorders in clinic. Due to the limited regeneration ability of tendons, tissue engineering technology is often used as an effective approach to treat tendon injuries. Silk fibroin (SF) films have excellent biological activities and physical properties, which is suitable for tendon regeneration. The present study is aimed at preparing a SF film with a bionic microstructure and investigating its biological effects.

Methods: A SF film with a smooth surface or bionic microstructure was prepared. After seeding tendon stem/progenitor cells (TSPCs) on the surface, the cell morphology, the expression level of tenogenic genes and proteins, and the focal adhesion kinase (FAK) activation were measured to evaluate the biological effect of SF films.

Results: The TSPCs on SF films with a bionic microstructure exhibited a slender cell morphology, promoted the expression of tenogenic genes and proteins, such as SCX, TNC, TNMD, and COLIA1, and activated FAK. FAK inhibitors blocked the enhanced expression of tenogenic genes and proteins.

Conclusion: SF films with a bionic microstructure may serve as a scaffold, provide biophysical cues to alter the cellular adherence arrangement and cell morphology, and enhance the tenogenic gene and protein expression in TSPCs. FAK activation plays a key role during this biological response process.
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http://dx.doi.org/10.1155/2020/8857380DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7657703PMC
November 2020

RING finger protein RGLG1 and RGLG2 negatively modulate MAPKKK18 mediated drought stress tolerance in Arabidopsis.

J Integr Plant Biol 2021 Mar;63(3):484-493

State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an, 271018, China.

Mitogen activated protein kinase kinase kinase 18 (MAPKKK18) mediated signaling cascade plays important roles in Arabidopsis drought stress tolerance. However, the post-translational modulation patterns of MAPKKK18 are not characterized. In this study, we found that the protein level of MAPKKK18 was tightly controlled by the 26S proteasome. Ubiquitin ligases RGLG1 and RGLG2 ubiquitinated MAPKKK18 at lysine residue K32 and K154, and promoted its degradation. Deletion of RGLG1 and RGLG2 stabilized MAPKKK18 and further enhanced the drought stress tolerance of MAPKKK18-overexpression plants. Our data demonstrate that RGLG1 and RGLG2 negatively regulate MAPKKK18-mediated drought stress tolerance in Arabidopsis.
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http://dx.doi.org/10.1111/jipb.13019DOI Listing
March 2021

The absence of oestrogen receptor beta disturbs collagen I type deposition during Achilles tendon healing by regulating the IRF5-CCL3 axis.

J Cell Mol Med 2020 09 10;24(17):9925-9935. Epub 2020 Aug 10.

Department of Orthopedic Surgery, Southwest Hospital, Army Medical University, Chongqing, China.

Achilles tendon healing (ATH) remains an unanswered question in the field of sports medicine because it does not produce tissue with homology to the previously uninjured tissue. Oestrogen receptor β (ERβ) is involved in the injury and repair processes of tendons. Our previous study confirmed that ERβ plays a role in the early stage of ATH by affecting adipogenesis, but its role in extracellular matrix (ECM) remodelling is unknown. We established a 4-week Achilles tendon repair model to investigate the mechanism through which ERβ affects ATH at the very beginning of ECM remodelling phase. In vitro studies were performed using tendon-derived stem cells (TDSCs) due to their promising role in tendon healing. Behavioural and biomechanical tests revealed that ERβ-deficient mice exhibit weaker mobility and inferior biomechanical properties, and immunofluorescence staining and qRT-PCR showed that these mice exhibited an erroneous ECM composition, as mainly characterized by decreased collagen type I (Col I) deposition. The changes in gene expression profiles between ERβ-knockout and WT mice at 1 week were analysed by RNA sequencing to identify factors affecting Col I deposition. The results highlighted the IRF5-CCL3 axis, and this finding was verified with CCL3-treated TDSCs. These findings revealed that ERβ regulates Col I deposition during ATH via the IRF5-CCL3 axis.
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http://dx.doi.org/10.1111/jcmm.15592DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7520326PMC
September 2020

Efficient Tissue Culture Protocol for (Magnoliaceae) and Confirmation of Genetic Stability of the Regenerated Plants.

Plants (Basel) 2020 Aug 5;9(8). Epub 2020 Aug 5.

Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, Guangzhou 510642, China.

(Magnoliaceae) is classified as an endangered species by the International Union for Conservation of Nature. It has high commercial value owing to its attractive tree shape and flowers. We adopted an excellent genotype of as the parent material and established a mini-cut orchard through grafting to provide trunk shoots explants over the long-term. Optimal sterilization was achieved using a combination of 75% ethanol for 30 s, one percent benzalkonium bromide for five minutes, and 0.1% mercuric chloride for five minutes. Modified Murashige and Skoog medium (ML) was the optimal medium for the growth of . Addition of one mg/L of 6-benzyl adenine (BA) and 0.05 mg/L of α-naphthaleneacetic acid (NAA) to the medium increased the shoot induction rate to 95.56%, and the ML medium containing 0.4 mg/L BA and 0.04 mg/L NAA achieved the maximum multiplication rate (284.56%). Dark treatment for seven days, followed by continuous light treatment could better resolve the challenge of difficult rooting in plants. Using random amplified polymorphic DNA and inter simple sequence repeat markers, we confirmed the genetic uniformity and stability of the regenerated plants. Our protocol should be helpful for the propagation and conservation of this endangered plant.
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http://dx.doi.org/10.3390/plants9080997DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7465347PMC
August 2020
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